CN105284609B - A kind of cultural method of edible without hormone tissue culture roxburgh anoectochilus terminal bud - Google Patents
A kind of cultural method of edible without hormone tissue culture roxburgh anoectochilus terminal bud Download PDFInfo
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- CN105284609B CN105284609B CN201510367049.5A CN201510367049A CN105284609B CN 105284609 B CN105284609 B CN 105284609B CN 201510367049 A CN201510367049 A CN 201510367049A CN 105284609 B CN105284609 B CN 105284609B
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Abstract
The invention discloses a kind of cultural method of edible without hormone tissue culture roxburgh anoectochilus terminal bud, include the 20g+20 40g of 20 60g+ matrimony vines of MS+ bananas 50 100g+ corns, 15 55g+ potatoes, 40 80g+ mung beans, 80 150g+ soya beans, 20 80g+ jujubes 5 sugar per L culture medium raw materials.Cultural method is, the 3000Lux of light intensity 1500, the 14h of light application time 10,20 25 DEG C of temperature, under the conditions of grow 24 months.According to the inventive method, 11cm can be grown within 3 months.
Description
Technical field
The present invention relates to a kind of cultural method of edible without hormone tissue culture roxburgh anoectochilus terminal bud.
Background technology
Gold thread Nelumbo orchid, whole plant for medical use have the title of " king of medicine ".Have clearing heat and cooling blood, evacuate pathogenic wind dampness removing, cardiac stimulant profit
Urinate, reinforce the kidney, soothing the liver, improve immunity, reducing blood sugar and blood lipid, it is anti-oxidant the effects such as.But in natural environment, roxburgh anoectochilus terminal bud seed needs
It could be sprouted in the presence of endogenetic fungus, strict to ecological environment requirement, self-reproduction coefficient is low.Current Plant Tissue Breeding
Technology greatly expands culture and shortened often adds some hormones to promote in the roxburgh anoectochilus terminal bud cultivation time, but tissue culture toward culture medium
Enter and commercial seedling requirement is reached in the roxburgh anoectochilus terminal bud short period.In tissue culture commonly use growth hormone have dichlorphenoxyacetic acid (2,4-D),
Methyl α-naphthyl acetate (NAA), heteroauxin (IAA), indole -3-butyric acid (IBA), conventional cell division have 6-benzyl aminopurine
(6-BA), kinetin (6-Furfurylaminopurine, KT), zeatin (ZT), thidiazuron (thidiazuron, TDZ).These
Harm of the hormone to human body, mainly by the normal hormonal " with reference to " in material and human body containing this hormonal components, draws
Endocrine disturbance is sent out, causes human normal hormone not normal, shows many aspects such as development, reproduction, spirit, mood.If group
Training roxburgh anoectochilus terminal bud decomposes these auxin without the plantation of more than 3 months of sylvan life Ecology, directly takes away and does dry product, as a result
The health of eater will be damaged.
The content of the invention
It is an object of the invention to provide a kind of cultural method of edible without hormone tissue culture roxburgh anoectochilus terminal bud, to solve existing skill
Above mentioned problem present in art, reach tissue culture roxburgh anoectochilus terminal bud can direct safe edible purpose.
The technical scheme that the present invention is provided is as follows：
A kind of cultural method of edible without hormone tissue culture roxburgh anoectochilus terminal bud, comprises the following steps：
1) prepared by culture medium
Banana dials skin chopping, is put into juice extractor and is broken into mud, and such as juice extractor effect is bad to be added appropriate water and (be less than
50ml/500g)；
Potato chopping of peeling is put into juice extractor and is broken into mud；
Mung bean soybean, corn, chooses mung bean, soya bean and the corn of full seed, invades bubble 6-20 hours, takes out, is put into and squeezes the juice
It is beaten in machine；The preferred proportion of mung bean, soya bean and corn and water is respectively 1:2-5 volume of water.
Removing Kernel for Date, is put into mashed into paste in beating crusher；Mashed into paste in matrimony vine, mortar；
Without hormone edible roxburgh anoectochilus terminal bud culture medium prescription:MS+ banana 50-100g+ corn 15-55g+ potatoes 40-80g+ is green
Beans 80-150g+ soya bean 20-80g+ jujube 20-60g+ matrimony vines 5-20g+2-4% sugar+0.5-1.5% agar, uses water constant volume
To 1L；Heating stirring, culture medium is made；
2) roxburgh anoectochilus terminal bud plant is cleaned up, roxburgh anoectochilus terminal bud is sheared close to stipes 0.5-1.5cm or so；
3) roxburgh anoectochilus terminal bud after shearing is inserted into culture medium；
4) in light intensity 1500-3000Lux, light application time 10-14h is grown under the conditions of 20-25 DEG C of temperature.
In the present invention, it is preferred to be used as culture vessel using blake bottle.
In the present invention, the volume of blake bottle is 50ml-1L.
In the present invention, culture volume is 1/10-1/3 in each blake bottle.
In the present invention, step 4) incubation time is 2-4 months.
Sugar used in the present invention is sucrose.
Advantages of the present invention is as follows：
1st, the roxburgh anoectochilus terminal bud culture medium that the present invention is used is for pure natural food materials, without hormone prescription, and the roxburgh anoectochilus terminal bud that tissue culture goes out is without sharp
Element influence, it is safe to eat.
2nd, without the plantation of more than 3 months of field Ecology, the time of roxburgh anoectochilus terminal bud listing can be greatly shortened, is reduced into
This；And incubation can be carried out indoors, it is easy to management；
3rd, fast growth, plant height can grow into 11cm from 1-2cm or so section within 3 months.
Brief description of the drawings
Fig. 1 is the photo in kind of the embodiment of the present invention 4；
Embodiment
The roxburgh anoectochilus terminal bud that all examples below is used is both from Taiwan, the ripe plant after domestication.
Embodiment 1
1st, plant is cleaned up, flowing water is rinsed half an hour, about 0.5- is sheared in sterilization centered on roxburgh anoectochilus terminal bud node
The stipes of 2.0cm length；
2 bananas, choose banana (Taiwan any of several broadleaf plants, Zhangzhou City of Fujian Province) and dial skin chopping, be put into juice extractor and be broken into mud and (such as squeeze the juice
Machine effect is bad to add appropriate water, 40ml/500g)；
3 potatoes, the potato that in the market is sold, chopping of peeling is put into juice extractor and is broken into mud (such as juice extractor effect is bad can
Add appropriate water, 30ml/500g)
4 mung bean soybean, corns, choose mung bean, soya bean and the corn of full seed respectively, invade bubble 10h hours or so, take out
It is put into pallet, is put into juice extractor and breaks into pulpous state, the ratio that adds water is 1:3 times of volume of water.
5. Removing Kernel for Date, is put into mashed into paste in beating crusher
6. mashed into paste (5ml water is added in every 20 grams) in matrimony vine, mortar
Without hormone edible roxburgh anoectochilus terminal bud culture medium prescription:MS+ banana 40g+ corn 55g+ potato 45g+ mung bean 150g+ soya beans
The agar of 20g+ jujube 20g+ matrimony vines 5g+3% sugar+0.7%, with water constant volume to 1L
Banana 50g+ corn 55g+ potato 40g+ mung bean 150g+ soya bean 20g+ jujube 20g+ matrimony vines 5g+40g sugar (is made
Sugar is sucrose, and following examples are identical).
In light intensity 2000Lux, light application time 12h is cultivated 3 months under the conditions of 22 DEG C of temperature.
Embodiment 2
Its plant is cleaned up first, flowing water is rinsed half an hour, sterilization is sheared about centered on roxburgh anoectochilus terminal bud node
The stipes of 0.5-2.0cm length；
2 bananas, choose still immature banana (Zhangzhou City of any of several broadleaf plants Fujian Province, Taiwan) and dial skin chopping, be put into juice extractor and be broken into
Mud (such as juice extractor effect is bad to add appropriate water, 40ml/500g)
3 potatoes, the potato that in the market is sold, chopping of peeling is put into juice extractor and is broken into mud (such as juice extractor effect is bad can
Add appropriate water, 30ml/500g)
4 mung bean soybean, corns, choose mung bean, soya bean and the corn of full seed respectively, invade bubble 12h hours or so, take out
It is put into pallet, is put into juice extractor and breaks into pulpous state, the ratio that adds water is 1:2 times of volume of water.
5. Removing Kernel for Date, is put into mashed into paste in beating crusher
6. mashed into paste (5ml water is added in every 20 grams) in matrimony vine, mortar
Without hormone edible roxburgh anoectochilus terminal bud culture medium prescription:MS+ banana 100g+ corn 15g+ potato 40g+ mung bean 80g+ soya beans
The agar of 80g+ jujube 60g+ matrimony vines 15g+30g sucrose+0.7%, with water constant volume to 1L
Banana 90g+ corn 35g+ potato 750g+ mung bean 90g+ soya bean 48g+ jujube 43g+ matrimony vines 18g+28g sugar.
In light intensity 1800Lux, light application time 11h is cultivated 3 months under the conditions of 20 DEG C of temperature.
Embodiment 3
Roxburgh anoectochilus terminal bud kind comes from Taiwan, is tamed, and first cleans up its plant, and flowing water rinses half an hour,
Sterilization, shears the stipes of about 0.5-2.0cm length centered on roxburgh anoectochilus terminal bud node；
2 bananas, choose still immature banana (Zhangzhou City of any of several broadleaf plants Fujian Province, Taiwan) and dial skin chopping, be put into juice extractor and be broken into
Mud (such as juice extractor effect is bad adds appropriate water 40ml/500g)
3 potatoes, the potato that in the market is sold, chopping of peeling is put into juice extractor and is broken into mud (such as juice extractor effect is bad can
Add appropriate water 30ml/500g)
4 mung bean soybean, corns, choose mung bean, soya bean and the corn of full seed respectively, invade bubble 10h hours or so, take out
It is put into pallet, is put into juice extractor and breaks into pulpous state, the ratio that adds water is 1:1 times of volume of water.
5. Removing Kernel for Date, is put into mashed into paste in beating crusher
6. mashed into paste (5ml water is added in 20 grams) in matrimony vine, mortar
Without hormone edible roxburgh anoectochilus terminal bud culture medium prescription:MS+ banana 60g+ corn 20g+ potato 55g+ mung bean 120g+ soya beans
The agar of 70g+ jujube 50g+ matrimony vines 15g+35g sugar+0.7%, with water constant volume to 1L
Banana 100g+ corn 25g+ potato 70g+ mung bean 90g+ soya bean 350g+ jujube 350g+ matrimony vines 5-20g+35g sugar.
In light intensity 1800Lux, light application time 11h is cultivated 3 months under the conditions of 20 DEG C of temperature.
Embodiment 4
Roxburgh anoectochilus terminal bud kind comes from Taiwan, is tamed, and first cleans up its plant, and flowing water rinses half an hour,
Sterilization, shears the stipes of about 0.5-2.0cm length centered on roxburgh anoectochilus terminal bud node；
2 bananas, choose still immature banana (Zhangzhou City of any of several broadleaf plants Fujian Province, Taiwan) and dial skin chopping, be put into juice extractor and be broken into
Mud (such as juice extractor effect is bad adds appropriate water 40ml/500g)
3 potatoes, the potato that in the market is sold, chopping of peeling is put into juice extractor and is broken into mud (such as juice extractor effect is bad can
Add appropriate water 30ml/500g)
4 mung bean soybean, corns, choose mung bean, soya bean and the corn of full seed respectively, invade bubble 12h hours or so, take out
It is put into pallet, is put into juice extractor and breaks into pulpous state, the ratio that adds water is 1:2 times of volume of water.
5. Removing Kernel for Date, is put into mashed into paste in beating crusher
6. mashed into paste (5ml water is added in 20 grams) in matrimony vine, mortar
Without hormone edible roxburgh anoectochilus terminal bud culture medium prescription:MS+ banana 65g+ corn 35g+ potato 65g+ mung bean 115g+ soya beans
The agar of 50g+ jujube 50g+ matrimony vines 20g+25g sugar+0.7%, with water constant volume to 1L
Banana 75g+ corn 40g+ potato 60g+ mung bean 100g+ soya bean 60g+ jujube 35g+ matrimony vines 15g+30g sugar.
In light intensity 1800Lux, light application time 11h is cultivated 3 months under the conditions of 20 DEG C of temperature.Plant grows to about 11cm length, stem
Leaf thickness is normal, and form is normal, sees Fig. 1.
Claims (4)
1. a kind of cultural method of edible without hormone tissue culture roxburgh anoectochilus terminal bud, comprises the following steps：
1）It is prepared by culture medium：
Banana peeling is shredded, and is put into juice extractor and is broken into mud；Potato chopping of peeling is put into juice extractor and is broken into mud；
Mung bean soybean, corn, chooses mung bean, soya bean and the corn of full seed, soaks 6-20 hours, takes out, is put into juice extractor
Mashing；
Removing Kernel for Date, is put into mashed into paste in beating crusher；Mashed into paste in matrimony vine, mortar；
Without hormone edible roxburgh anoectochilus terminal bud culture medium prescription:MS+ bananas 50-100g+corn 15-55g+potato 40-80g+ is green
Beans 80-150g+ soya beans 20-80g+jujube 20-60g+ matrimony vine 5-20g+2-4% sucrose+0.5-1.5% agar, is arrived with water constant volume
1L；Heating stirring, culture medium is made；
2）Roxburgh anoectochilus terminal bud plant is cleaned up, sterilized, the stipes of 0.5-2.0cm length is sheared centered on roxburgh anoectochilus terminal bud node；
3）Roxburgh anoectochilus terminal bud stipes after shearing is linked into culture medium；
4）In light intensity 1500-3000Lux, light application time 10-14h is grown under conditions of 20-25 DEG C of temperature, the time is 2-4
Month.
2. a kind of edible as claimed in claim 1 is without hormone tissue culture roxburgh anoectochilus terminal bud cultural method, it is characterised in that：Culture vessel
For blake bottle.
3. a kind of edible as claimed in claim 2 is without hormone tissue culture roxburgh anoectochilus terminal bud cultural method, it is characterised in that：Blake bottle
Volume is 50ml-1L.
4. a kind of edible as claimed in claim 2 is without hormone tissue culture roxburgh anoectochilus terminal bud cultural method, it is characterised in that：Each culture
Culture volume is 1/10-1/3 in bottle.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106134998B (en) * | 2016-07-13 | 2018-01-16 | 云南英纽生物科技有限公司 | A kind of tissue culture medium for anoectochilus roxburghii and its preparation method and application |
| CN106258957B (en) * | 2016-08-08 | 2018-05-25 | 四川千草生物技术股份有限公司 | A kind of roxburgh anoectochilus terminal bud is without hormone containerization cultural method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416310A (en) * | 2013-08-28 | 2013-12-04 | 叶宝鑫 | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture |
| CN103999774A (en) * | 2014-05-29 | 2014-08-27 | 杭州虹越花卉有限公司 | Formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings by one step |
| CN104429965A (en) * | 2014-12-16 | 2015-03-25 | 四川省自然资源科学研究院 | Hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds |
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| KR101348905B1 (en) * | 2011-11-11 | 2014-01-09 | 경상남도 | Transformation Method using by Seed of Orchids |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416310A (en) * | 2013-08-28 | 2013-12-04 | 叶宝鑫 | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture |
| CN103999774A (en) * | 2014-05-29 | 2014-08-27 | 杭州虹越花卉有限公司 | Formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings by one step |
| CN104429965A (en) * | 2014-12-16 | 2015-03-25 | 四川省自然资源科学研究院 | Hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds |
Non-Patent Citations (2)
| Title |
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| Micropropagation of an endangered orchid Anoectochilus formosanus;N.V. KET et.al.,;《BIOLOGIA PLANTARUM》;20041231;第48卷(第3期);第339-344页 * |
| 适宜工厂化生产的江西野生金线莲组织培养技术研究;王莹等;《农业与技术》;20140331;第34卷(第3期);摘要，第11页左栏第3段 * |
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Address after: 361000 No. 1999 Middle Road, Jimei District, Fujian, Xiamen Patentee after: Xiamen Medical College Address before: Siming District of Xiamen city in Fujian province 361000 Qianpu Rock Road No. 8 Patentee before: Xiamen Medical College |