CN105274183B - A method of determining river water quality grade - Google Patents

A method of determining river water quality grade Download PDF

Info

Publication number
CN105274183B
CN105274183B CN201510659509.1A CN201510659509A CN105274183B CN 105274183 B CN105274183 B CN 105274183B CN 201510659509 A CN201510659509 A CN 201510659509A CN 105274183 B CN105274183 B CN 105274183B
Authority
CN
China
Prior art keywords
water quality
percentage
algae
chain
melosira
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201510659509.1A
Other languages
Chinese (zh)
Other versions
CN105274183A (en
Inventor
王超
赖子尼
李新辉
杨婉玲
曾艳艺
李海燕
高原
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pearl River Fisheries Research Institute CAFS
Original Assignee
Pearl River Fisheries Research Institute CAFS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pearl River Fisheries Research Institute CAFS filed Critical Pearl River Fisheries Research Institute CAFS
Priority to CN201510659509.1A priority Critical patent/CN105274183B/en
Priority to PCT/CN2015/092452 priority patent/WO2017063206A1/en
Publication of CN105274183A publication Critical patent/CN105274183A/en
Application granted granted Critical
Publication of CN105274183B publication Critical patent/CN105274183B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18Water

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Seaweed (AREA)

Abstract

The invention discloses a kind of method for determining river water quality grade, this method is the percentage X for measuring curved particle melosira in water body and accounting for all particle melosiras;If percentage X < 10%, water quality is I class water quality;If percentage X is 10%≤X < 20%, water quality is II class water quality;If percentage X is 20%≤X < 40%, water quality is Group III water quality;If percentage X is 40%≤X < 50%, water quality is IV class water quality;If percentage X is 50%≤X < 60%, water quality is V class water quality;If percentage X >=60%, water quality is bad V class water quality.The present invention carries out microexamination by the form to the particle melosira in rivers water body, intuitively differentiates and count the percentage composition of bending algae chain, to judge the water quality nutrition grade of investigation river section.The method is not high to the specialized capability of operator and the configuration requirement of instrument and equipment, has preferable practicability, and generalization is strong.

Description

A method of determining river water quality grade
Technical field
The present invention relates to a kind of methods for determining river water quality grade.
Background technique
Particle melosira (current latin nameAulacoseira granulata;Once latin name is usedMelosira granulata) belong to Bacillariophyta center guiding principle Melosira, it is the common advantage algae in poisons in freshwater, worldwide extensively Distribution (Karim and Saeed, 1978; Höetzel and Croome, 1996;Nakano et al., 1996; O' Farrell et al., 2001; Godlewska et al., 2003; Tsukada et al., 2006).Algae chain end Have the separate housing (Florin, 1970) of lunge, becomes the important evidence of taxonomic identification under optical microscopy.
According to " CHINESE FRESHWATER algae will, Volume Four, Bacillariophyta, center guiding principle " (Qi Yuzao, 1991), the algae chain of particle melosira Form is in " three mutation and a modification ": former mutation (var.granulata), extremely narrow mutation (var.angustissima), extremely narrow mutation spiral modification (var. angustissima f. spiralis) and bending mutation (var.curvata)。
Particle melosira form is related with water environment, can reflect water quality situation and situation of change.The cell of particle melosira It can change because of the influence of environmental factor with the shape size of algae chain, impact factor includes: nitrate nitrogen, total phosphorus (G mez et al., 1995;Turkia and Lepist, 1999), transparency (O ' Farrell et al., 2001), Grazing Pressure (Davey and Crawford, 1986), silicate concentration (Stoermeret al., 1981;Davey, 1987), water temperature (Davey, 1987;G mez et al., 1995), photoperiod (Davey, 1987), water column stability (G mez etal., 1995)、CO2Content (TallingandRz ska, 1967) and calcium concentration (G mezet al., 1995).
The prior art is mainly from the Monopterus albus of particle melosira, cell size (including cell dia, length and body Product) and algae chain size (cell number and length) study its instruction function to water body eutrophication degree.To researcher's Specialized capability and research equipment have higher requirement, and target user is few, and time-consuming and laborious, and difference research waters is finally obtained Research conclusion is also not quite similar.
The specifying information of the above-mentioned bibliography being related to is as follows:
Qi Yuzao chief editor, 1995.CHINESE FRESHWATER algae will, Volume Four, Bacillariophyta, center guiding principle.Science Press, 13-17 Page.
Davey M C, 1987. Seasonal variation in the filament morphology of the freshwater diatom
Melosira granulata (Ehr) Ralfs. Freshwater Biology, 18: 5-16.
Davey M C and Crawford R M, 1986. Filament formation in the diatomMelosira granulata.
Journal of Phycology, 22: 144-150.
Godlewska M, Mazurkiewicz-Boroń G, Pociecha A, Wilk-Woźniak E, Jelonek M., 2003. Effects of
flood on the functioning of the Dobczyce reservoir ecosystem. Hydrobiologia, 504: 305-313.
Gómez N, Riera J L, Sabater S, 1995. Ecology and morphological variability of Aulacoseira
granulata (Bacillariophyceae) in Spanish reservoirs. Journal of Plankton Research, 17: 1-16.
Höetzel G and Croome R., 1996. Population dynamics of Aulacoseira granulata (Ehr.) Simonson
(Bacillariophyceae, Centrales), the dominant alga in the Murray River, Australia. Archiv fuer
Hydrobiologie, 136: 191-215.
Karim A G A, Saeed O M., 1978. Studies on the freshwater algae of the Sudan Ⅲ, vertical distribution of Melosira granulata (Ehr) ralfs. In the White Nile, with reference to certain environmental variables. Hydrobiologia, 57: 73-79.
Nakano S, Seike Y, Sekino T, Okumura M, Kawabata K, Fujinaga K, Nakanishi M, Mitamura O,
Kumagai M, Hashitani H., 1996. A rapid growth of Aulacoseira granulata (Bacillariophyceae)
during the typhoon season in the South Basin of Lake Biwa. Japanese Journal of Limnology,
57: 493-500.
O’Farrell I, Tell G, Podlejski A, 2001. Morphological variability ofAulacoseira granulata (Ehr)
Simonsen (Bacillariophyceae) in the lower Paraná River (Argentina). Limnology, 2: 65-71.
Tsukada H, Tsujimura S, Nakahara H., 2006. Seasonal succession of phytoplankton in Lake Yogo
over 2 years: effect of artificial manipulation. Limnology, 7: 3-14.
Stoermer E F, Kreis R G, Sicko-Goad L, 1981. A systematic, quantitative and ecological
comparison of Melosira islandica O. Müll. with M. granulata (Ehr) Ralfs from the Laurentian
Great Lakes. Journal of Great Lakes Research, 7: 345-356.
Talling J F and Rzόska J, 1967. The development of plankton in relation to hydrological regime in the Blue Nile. Journal of Ecology, 55: 637-662.
Turkia J, Lepisto L, 1999. Size variations of planktonic Aulacoseira Thwaites (Diatomae) in water and in sediment from Finnish lakes of varying trophic state. Journal of Plankton Research, 21(4): 757-770。
Summary of the invention
In order to solve above-mentioned disadvantage, the present invention only need to be observed and be counted from intuitive morphological feature The arc-shaped bend algae chain of grain melosira original mutation proportion in all algae chains is the nutrition grade that can determine whether water quality;The present invention Method is not high to the specialized capability of practitioner and the requirement of instrument and equipment, time saving and energy saving, wide application of the crowd, have promote and The meaning of science popularization.
The purpose of the present invention is to provide a kind of methods for determining river water quality grade.
The technical solution used in the present invention is:
A method of determining water grade, it is characterised in that: this method is particle melosira original mutation in measurement water body Bending shape algae chain quantity account for all particle melosira original mutation algae chain quantity percentage X;If percentage X < 10%, water Matter is I class water quality;If percentage X is 10%≤X < 20%, water quality is II class water quality;If percentage X is 20%≤X < 40%, water quality For Group III water quality;If percentage X is 40%≤X < 50%, water quality is IV class water quality;If percentage X is 50%≤X < 60%, water Matter is V class water quality;If percentage X >=60%, water quality is bad V class water quality.
Further, above-mentioned water quality is surface water quality.
Further, above-mentioned water quality is river water quality.
Further, the bending shape algae chain of above-mentioned particle melosira original mutation is the algae chain in an arc-shaped bend, and It can be regarded as the bending shape algae chain effectively measured in highly/span >=0.015.
The beneficial effects of the present invention are:
1) present invention discover that particle melosira original mutation accounts for all particle melosira original mutation in the curved algae chain of arc line shaped Percentage there are obvious relation between persistence with rivers Different Nutrition grade water body, in the rivers water body of I class water quality, arc line shaped be bent algae Percentage X < 10% in all algae chains shared by chain;In the rivers water body of II class water quality, arc line shaped is bent algae chain percentage X For 10%≤X < 20%;In the rivers water body of Group III water quality, it is 20%≤X < 40% that arc line shaped, which is bent algae chain percentage X,; In the rivers water body of IV class water quality, it is 40%≤X < 50% that arc line shaped, which is bent algae chain percentage X,;In the rivers of V class water quality In water body, it is 50%≤X < 60% that arc line shaped, which is bent algae chain percentage X,;In the rivers water body of bad V class water quality, arc line shaped It is bent algae chain percentage X >=60%.
2) present invention passes through to the microscopic morphology observation of particle melosira original mutation in Different Nutrition grade water body and difference The measurement of morphological parameters, and compare the relationship between different shape parameter and water nutrition grade, it finally found that arc-shaped bend It is best to the indicative function of water nutrition grade that algae chain accounts for all particle melosira percentages.Therefore, based on of the invention as a result, The percentage for only needing to observe and count arc line shaped bending algae chain in the original mutation of particle melosira can differentiate the battalion of rivers water body Grade is supported, simple and easy, efficient, replicability is strong.
3) present invention carries out microexamination by the form to the particle melosira in rivers water body, intuitively differentiates and unites The percentage composition of meter bending algae chain, to judge the water quality nutrition grade of investigation river section.Profession element of the method to operator The configuration requirement of matter and instrument and equipment is not high, has preferable practicability, and generalization is strong.
Detailed description of the invention
Fig. 1 is the microexamination and morphological parameters instrumentation plan of particle melosira original mutation straight chain form;
Fig. 2 is the microexamination and morphological parameters instrumentation plan of particle melosira original mutation arc-shaped bend form;
Fig. 3 is the bending degree instrumentation plan of particle melosira original mutation arc-shaped bend form.
Specific embodiment
Present invention research early period is to the microscopic morphology observation and difference by particle melosira in Different Nutrition grade water body The measurement of morphological parameters, and the relationship between more various different shape parameters and water nutrition grade, by largely studying It is best to the indicative function of water nutrition grade to finally found that arc-shaped bend algae chain accounts for all particle melosira percentages.
A method of determining water grade, it is characterised in that: this method is particle melosira original mutation in measurement water body Bending shape algae chain quantity account for all particle melosira original mutation algae chain quantity percentage X;If percentage X < 10%, water Matter is I class water quality;If percentage X is 10%≤X < 20%, water quality is II class water quality;If percentage X is 20%≤X < 40%, water quality For Group III water quality;If percentage X is 40%≤X < 50%, water quality is IV class water quality;If percentage X is 50%≤X < 60%, water Matter is V class water quality;If percentage X >=60%, water quality is bad V class water quality.
Preferably, above-mentioned water quality is surface water quality.
Preferably, above-mentioned water quality is river water quality.
Preferably, the bending shape algae chain of above-mentioned particle melosira original mutation is the algae chain in an arc-shaped bend, and high It can be regarded as the bending shape algae chain effectively measured in degree/span >=0.015.
The present invention is further illustrated combined with specific embodiments below, and however, it is not limited to this.
Embodiment 1
The present embodiment carries out trawlnet repeatedly in the water body of investigation river section using No. 25 plankton nets, will be collected into Planktonic algae be placed in 100 milliliters of polyethylene bottles, and add Shandong Grignard solution fix, final fixed concentration be 15 ‰.Sample is taken back Behind laboratory, is dripped with suction pipe sampling and on glass slide, carry out microexamination, measurement record particle melosira after capping slide Former mutation quantity and Morphologic Parameters, percentage, the bending degree for accounting for all particle melosiras including arc-shaped bend algae chain are (high Degree/span), cell dia, cell length, algae chain cell number, algae chain length and terminal spine length.In the algae sample of each river section The former mutation algae chain sum of observation statistics >=100.After observation, statistics bending algae chain percentage, other parameters are asked Average value.The relationship of each parameter and different quality grade is compared, in the Morphologic Parameters for sending out acquisition currently all, Arc-shaped bend algae chain percentage can best reflect the water nutrition grade of different river sections, and this parameter is most easily by intuitively dividing Acquisition is distinguished, without carrying out complexity and accurately measuring.
The present invention is provided with sampled point, including Fengkai, Deqing, Zhaoqing, Qing Qi, a left side in 16 rivers of Zhujiang River middle and lower reaches section Beach off-lying sea, is newly enclosed, small olive, pulk, northern Kau, olive core, horizontal drip, Chen Cun, city's bridge, Lianhua Shan Mountain and Pearl River Bridge, covers I class to bad V Class water quality.Microexamination and measurement have been carried out to the morphological feature of the particle melosira of different river sections according to the above technical scheme, And the percentage for accounting for all particle melosiras to arc-shaped bend algae chain counts.
Testing result is as shown in Figures 1 to 3, and wherein Fig. 1 is the microexamination and shape of particle melosira original mutation straight chain form State parameter measurement schematic diagram is of a straight line type, algae chain it can be seen that the cell of the original mutation is linked to be close long catenoid colony Both ends tool thorn, 23.82 μm of algae chain width, 187.33 μm of algae chain length;Fig. 2 is particle melosira original mutation arc-shaped bend form Microexamination and morphological parameters instrumentation plan;Fig. 3 is that the bending degree of arc-shaped bend form particle melosira measures signal Figure;It can be seen that the original mutation is in the form of an arc-shaped bend, and 14.28 μm of height, 175.51 μm of span, height/ Span value is 0.081, is greater than 0.015.
The present embodiment is as shown in table 1 to the testing result of 16 river section samples of Zhujiang River middle and lower reaches, what the method for the present invention measured Experimental result with according to national water grade standard " People's Republic of China's water environment quality standard " (GB 3838- 2002) result measured is completely the same, illustrates the method for the present invention for evaluating water grade with good accuracy.
The testing result of 1 Zhujiang River middle and lower reaches of table, 16 river section samples
Note: arc-shaped bend algae chain percentage is that the particle melosira original mutation of arc-shaped bend accounts for all particle melosira originals changes The percentage of kind.
The present invention has found that the original mutation of particle melosira is in arc line shaped also by the measurement to other a large amount of different location water quality The percentage of curved algae chain exists with rivers Different Nutrition grade water body to be significantly associated with, in the rivers water body of I class water quality, Arc line shaped is bent the percentage X < 10% in all algae chains shared by algae chain;In the rivers water body of II class water quality, arc line shaped is bent algae Chain percentage X is 10%≤X < 20%;In the rivers water body of Group III water quality, arc line shaped is bent algae chain percentage X For 20%≤X < 40%;In the rivers water body of IV class water quality, it is 40%≤X < 50% that arc line shaped, which is bent algae chain percentage X,; In the rivers water body of V class water quality, it is 50%≤X < 60% that arc line shaped, which is bent algae chain percentage X,;In the river of bad V class water quality In river water body, it is as shown in table 2 that arc line shaped is bent algae chain percentage X >=60%().
2 arc line shaped of table is bent the relationship between algae chain percentage and water grade
Note: bending algae chain percentage is that the particle melosira of arc-shaped bend accounts for the percentage of all particle melosiras.
The above results show to be bent percentage composition and surface water quality nutrition grade close relation that algae chain accounts for all algae chains.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention, It should be equivalent substitute mode, be included within the scope of the present invention.

Claims (3)

1. a kind of method for determining water grade, it is characterised in that: this method is particle melosira original mutation in measurement water body Bending shape algae chain quantity accounts for the percentage X of the algae chain quantity of all particle melosira original mutation;If percentage X < 10%, water Matter is I class water quality;If percentage X is 10%≤X < 20%, water quality is II class water quality;If percentage X is 20%≤X < 40%, Water quality is Group III water quality;If percentage X is 40%≤X < 50%, water quality is IV class water quality;If percentage X is 50%≤X < 60%, water quality is V class water quality;If percentage X >=60%, water quality is bad V class water quality;
The water quality is river water quality;
The bending shape algae chain refers to that the bending shape of former mutation is in the algae chain of an arc-shaped bend, and height/span >= 0.015, then calculating is bending shape algae chain.
2. according to the method described in claim 1, it is characterized by: the water quality is surface water quality.
3. according to the method described in claim 1, it is characterized by: the bending shape algae chain of the particle melosira original mutation It is the algae chain in an arc-shaped bend, and the bending shape algae chain effectively measured be can be regarded as in height/span >=0.015.
CN201510659509.1A 2015-10-12 2015-10-12 A method of determining river water quality grade Active CN105274183B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201510659509.1A CN105274183B (en) 2015-10-12 2015-10-12 A method of determining river water quality grade
PCT/CN2015/092452 WO2017063206A1 (en) 2015-10-12 2015-10-21 Method of determining river water quality

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510659509.1A CN105274183B (en) 2015-10-12 2015-10-12 A method of determining river water quality grade

Publications (2)

Publication Number Publication Date
CN105274183A CN105274183A (en) 2016-01-27
CN105274183B true CN105274183B (en) 2019-01-29

Family

ID=55144011

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510659509.1A Active CN105274183B (en) 2015-10-12 2015-10-12 A method of determining river water quality grade

Country Status (2)

Country Link
CN (1) CN105274183B (en)
WO (1) WO2017063206A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107085083A (en) * 2017-06-05 2017-08-22 合肥永泰新型建材有限公司 A kind of quality of river water contamination detection method
CN107991449A (en) * 2017-11-17 2018-05-04 宁波水表股份有限公司 A kind of water supply detecting and controlling system and method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102978275A (en) * 2012-11-14 2013-03-20 中国水产科学研究院珠江水产研究所 Method for judging whether growth of floating algae inhibited by nutrition or not

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3715114A1 (en) * 1987-05-06 1988-11-17 Krause Hans METHOD AND DEVICE FOR DETECTING TOXICITY IN SURFACE WATERS AND IN DRINKING AND WATER WATER
JP3274541B2 (en) * 1993-07-06 2002-04-15 株式会社日立製作所 Water purification plant management support method and support system
CN1664579A (en) * 2004-12-31 2005-09-07 湖南大学 Application of spirogyra in testing toxicity of polluted waste water and testing method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102978275A (en) * 2012-11-14 2013-03-20 中国水产科学研究院珠江水产研究所 Method for judging whether growth of floating algae inhibited by nutrition or not

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Quantifying diatom aspirations: Mechanical properties of chain-forming species;Young et al;《Limnol. Oceanogr》;20121231;第57卷(第6期);摘要,图2,方法部分,图2,讨论部分
广东长潭水库富营养化与浮游植物分布特征;王超等;《湖泊科学》;20130906;第25卷(第5期);摘要,结果与分析
西江颗粒直链藻种群生态特性;王超等;《生态学报》;20120831;第32卷(第15期);摘要,第4794页第2段,讨论部分

Also Published As

Publication number Publication date
CN105274183A (en) 2016-01-27
WO2017063206A1 (en) 2017-04-20

Similar Documents

Publication Publication Date Title
Kiss et al. Centric diatoms of large rivers and tributaries in Hungary: morphology and biogeographic distribution
Ács et al. Investigation of benthic algal communities, especially diatoms of some Hungarian streams in connection with reference conditions of the Water Framework Directives
Tavernini et al. Physical factors and dissolved reactive silica affect phytoplankton community structure and dynamics in a lowland eutrophic river (Po river, Italy)
CN106682326A (en) Method for evaluating ecological restoration of city watercourse
Li et al. Analysis of cell concentration, volume concentration, and colony size of Microcystis via laser particle analyzer
CN105274183B (en) A method of determining river water quality grade
Wang et al. Phytoplankton and eutrophication degree assessment of Baiyangdian Lake Wetland, China
Marquardt et al. Type analysis of Achnanthidium minutissimum and A. catenatum and description of A. tropicocatenatum sp. nov.(Bacillariophyta), a common species in Brazilian reservoirs
Zhu et al. Development and testing of the phytoplankton biological integrity index (P-IBI) in dry and wet seasons for Lake Gehu
Carney et al. Chrysophycean cysts: indicators of eutrophication in the recent sediments of Frains Lake, Michigan, USA
Serra et al. Evaluation of laser in situ scattering instrument for measuring concentration of phytoplankton, purple sulfur bacteria, and suspended inorganic sediments in lakes
Mihaljević et al. Spatial Pattern of Phytoplankton Based on the Morphology‐Based Functional Approach along a River–Floodplain Gradient
Szczepocka et al. Diatom biomonitoring–scientific foundations, commonly discussed issues and frequently made errors
Wang et al. Seasonal variations of Aulacoseira granulata population abundance in the Pearl River Estuary
CN110295220A (en) A kind of microbiological indicator appraisal procedure of Mangrove Wetlands deposit health status
Mora et al. Epilithic diatom communities of selected streams from the Lerma-Chapala Basin, Central Mexico, with the description of two new species
Veselá et al. The diatom flora of ephemeral headwater streams in the Elbsandsteingebirge region of the Czech Republic
Nguyen et al. Evaluating Surface Water Quality Using Indexes of Water Quality and Plankton Diversity
Huang et al. Characteristics of phytoplankton community structure and indication to water quality in the lake in agricultural areas
Zhao et al. Oxic urban rivers as a potential source of atmospheric methane
Weinbauer et al. Seasonal variability of microbial respiration and bacterial and archaeal community composition in the upper twilight zone
Olszyński et al. Aulacoseira pseudomuzzanensis sp. nov. and other centric diatoms from post iron ore mining reservoirs in Poland
CN106086200A (en) A kind of method for setting up the free technical specification warning coefficient H with endoparasitism phycobiont abundance and coral bleaching of Corallium Japonicum Kishinouye
Xu et al. A study on the phytoplankton community structure in the Diaohe River section of the Middle Route of the South-to-North Water Diversion Project in winter
CN103234865B (en) Microbial aggregate quantitative microscopic imaging testing and evaluating method

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant