CN105273093A - Preparation method for sulfated polysaccharides from seaweed - Google Patents
Preparation method for sulfated polysaccharides from seaweed Download PDFInfo
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- CN105273093A CN105273093A CN201410328156.2A CN201410328156A CN105273093A CN 105273093 A CN105273093 A CN 105273093A CN 201410328156 A CN201410328156 A CN 201410328156A CN 105273093 A CN105273093 A CN 105273093A
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Abstract
The invention discloses a preparation method for sulfated polysaccharides from seaweed. The sulfated polysaccharides from seaweed are prepared from sea-tangle through pretreatment, two-step water extraction, two-step alcohol precipitation, one-step alcohol dissolving and drying. The preparation method can effectively extract the seaweed sulfated polysaccharides in the sea-tangle and is capable of improving efficiency and yield of the seaweed sulfated polysaccharides through elaborate designing of every technological parameter.
Description
Technical field
The present invention relates to the extracting method of medicine, particularly relate to a kind of preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS.
Background technology
Different from the similar variety production technique through chemically modified or chemical process, SULFATED POLYSACCHARIDES FROM SEAWEEDS preparation method adopts natural bioactive technology.In extraction separation and purification process, do not add lead hydroxide, calcium chloride, precipitation agent and the organic reagents such as quaternary amine, ether, acetone, also soda acid processing or sulphonation chemistry are not modified, and only need drinkable water and medical ethanol two kinds to prepare medium.This technique neither destroys the natural chemical constitution of efficient part, turn avoid pollution that Isolation and purification process brings and strong acid, highly basic to the detrimentally affect of medicine.
At extraction SULFATED POLYSACCHARIDES FROM SEAWEEDS active principle simultaneously, other non-active ingredients, as iodine, algin (alginic acid and its esters), N.F,USP MANNITOL etc. need effectively to remove.
Sea-tangle is comparatively large in northern China marine site output, can establishing in large scale.Sea-tangle is included in Pharmacopoeia of People's Republic of China one (195 pages, version in 2010) kelp, is the dry thallus of Laminariaceae plant sea-tangle (LaminariajaponicaAresch).Its proterties is curlingly be folded into bulk, or tangles into handle, and all in chocolate or green and brown look, surface is with bloom.Soft with water logging, expand into flat long ribbon shape, long 50-150 centimetre, wide 10-40 centimetre, middle part is thicker, and edge is thinner and in wavy.Function with cure mainly as softening and resolving hard mass, dissolving phlegm, Li Shui.For goiter and tumor, scrofula, painful and swollen testis, phlegm and retained fluid oedema.And marine alga is the dry frond of Sargassaceae plant Sargassum or Sargassum fusiforme, again practise claim large leaflet marine alga, the shrinkage of marine alga proterties is curling, chocolate, have by bloom, long 30-60 centimetre.
Generally all adopt marine alga to be that raw material extracts SULFATED POLYSACCHARIDES FROM SEAWEEDS in prior art, this method one to make full use of the larger sea-tangle of northern China marine site output, and two is that its extracting method is not suitable for sea-tangle.
Summary of the invention
The object of this invention is to provide a kind of preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS, to being that SULFATED POLYSACCHARIDES FROM SEAWEEDS prepared by raw material with sea-tangle.
The preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS of the present invention, comprises the following steps:
S10, sea-tangle be placed in himself quality 38-42 water doubly, soak 35-45 minute after, the water for himself quality 38-42 times amount is rinsed well;
S20, pre-treatment is carried out to sea-tangle, reject the defective sea-tangle that leaf is yellow, leaf is broken and mouldy, qualified sea-tangle is cut into the fragment of area 7-10 square centimeter; Because sea-tangle is long ribbon shape, be applicable to adopting chopping processes to cut into pieces and be conducive to raising the efficiency and productive rate.
S30, sea-tangle joined decoct three times in himself quality 9-11 water doubly, be respectively 1.9-2.1 hour, 1.4-1.6 hour, 1.4-1.6 hour; SULFATED POLYSACCHARIDES FROM SEAWEEDS in sea-tangle can extract by SULFATED POLYSACCHARIDES FROM SEAWEEDS more fully that adopt the mode decocted to extract in sea-tangle.
S40, three decoction liquor are merged after be evaporated to relative density 1.05-1.15 and obtain concentrated solution I; Owing to having carried out concentrating under reduced pressure to decoction liquor, the concentration of SULFATED POLYSACCHARIDES FROM SEAWEEDS in concentrated solution has been increased, SULFATED POLYSACCHARIDES FROM SEAWEEDS can be extracted more easily, not only save ethanol, and the productive rate extracted is high.
S50, in concentrated solution I, add ethanol to ethanol contend concentration 29-31%, centrifugal supernatant liquor and sediment after hold over night;
S60, sediment to be detected, if SO in sediment
4 2-mass percentage be greater than 5%, in sediment, add ethanol to ethanol contend concentration 29-31%, leave standstill centrifugal supernatant liquor to layering; If SO in sediment
4 2 -mass percentage be less than or equal to 5%, abandon sediment; SULFATED POLYSACCHARIDES FROM SEAWEEDS in sediment can be extracted by this step, make the content of SULFATED POLYSACCHARIDES FROM SEAWEEDS in the sediment abandoned lower.
S70, by step S50 and step S60 supernatant liquor merge, be evaporated to relative density 1.1-1.2 and obtain concentrated solution II;
S80, in concentrated solution II, add ethanol to ethanol contend concentration 83-86%, after hold over night, abandon supernatant liquor, centrifugal after the washing with alcohol of precipitation percent by volume 74-76%;
S90, obtain SULFATED POLYSACCHARIDES FROM SEAWEEDS by carrying out spraying dry after the water dissolution of the precipitation 7-9 times amount after centrifugal.
By two above-mentioned step water extractions, the production line of the safety molten with a step alcohol, science, succinct extraction, abstraction and purification analysed by two step alcohol, SULFATED POLYSACCHARIDES FROM SEAWEEDS in sea-tangle effectively can be extracted, and pass through the well-designed of each processing parameter, efficiency and the productive rate of SULFATED POLYSACCHARIDES FROM SEAWEEDS can be improved.
Preferably, be placed in by sea-tangle in described step S10 in the water of himself quality 40 times and soak after 40 minutes, the water for himself quality 40 times amount is rinsed well.
Preferably, in described step S20, qualified sea-tangle is cut into the fragment of area 9 square centimeters.
Preferably, in described step S30, sea-tangle is joined and decocts three times in the water of himself quality 10, be respectively 2 hours, 1.5 hours, 1.5 hours.
Preferably, in described step S40, be evaporated to relative density 1.1 after three decoction liquor being merged and obtain concentrated solution I.
Preferably, in described step S50, in concentrated solution I, add ethanol to ethanol contend concentration 30%, centrifugal supernatant liquor and sediment after hold over night.
Preferably, in described step S60, if SO in sediment
4 2-mass percentage be greater than 5%, in sediment, add ethanol to ethanol contend concentration 30%, leave standstill centrifugal supernatant liquor to layering.
Preferably, in described step S70, the supernatant liquor in step S50 and step S60 is merged, be evaporated to relative density 1.17 and obtain concentrated solution II.
Preferably, in described step S80, in concentrated solution II, add ethanol to ethanol contend concentration 85%, after hold over night, abandon supernatant liquor, centrifugal after the washing with alcohol of precipitation percent by volume 75%.
Preferably, in described step S90, the precipitation after centrifugal is obtained SULFATED POLYSACCHARIDES FROM SEAWEEDS with carrying out spraying dry after the water dissolution of 8 times amount.
The preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS of the present invention effectively can extract the SULFATED POLYSACCHARIDES FROM SEAWEEDS in sea-tangle, has safety, science, succinct advantage.
Accompanying drawing explanation
The SULFATED POLYSACCHARIDES FROM SEAWEEDS monose composition gas chromatogram of Fig. 1 obtained by embodiment 1.
Embodiment
The preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS of the present invention, comprises the following steps:
S10, sea-tangle be placed in himself quality 38-42 water doubly, soak 35-45 minute after, the water for himself quality 38-42 times amount is rinsed well;
S20, pre-treatment is carried out to sea-tangle, reject the defective sea-tangle that leaf is yellow, leaf is broken and mouldy, qualified sea-tangle is cut into the fragment of area 7-10 square centimeter; Because sea-tangle is long ribbon shape, be applicable to adopting chopping processes to cut into pieces and be conducive to raising the efficiency and productive rate.
S30, sea-tangle joined decoct three times in himself quality 9-11 water doubly, be respectively 1.9-2.1 hour, 1.4-1.6 hour, 1.4-1.6 hour; SULFATED POLYSACCHARIDES FROM SEAWEEDS in sea-tangle can extract by SULFATED POLYSACCHARIDES FROM SEAWEEDS more fully that adopt the mode decocted to extract in sea-tangle.
S40, three decoction liquor are merged after be evaporated to relative density 1.05-1.15 and obtain concentrated solution I; Owing to having carried out concentrating under reduced pressure to decoction liquor, the concentration of SULFATED POLYSACCHARIDES FROM SEAWEEDS in concentrated solution has been increased, SULFATED POLYSACCHARIDES FROM SEAWEEDS can be extracted more easily, not only save ethanol, and the productive rate extracted is high.
S50, in concentrated solution I, add ethanol to ethanol contend concentration 29-31%, centrifugal supernatant liquor and sediment after hold over night;
S60, sediment to be detected, if SO in sediment
4 2-mass percentage be greater than 5%, in sediment, add ethanol to ethanol contend concentration 29-31%, leave standstill centrifugal supernatant liquor to layering; If SO in sediment
4 2 -mass percentage be less than or equal to 5%, abandon sediment; SULFATED POLYSACCHARIDES FROM SEAWEEDS in sediment can be extracted by this step, make the content of SULFATED POLYSACCHARIDES FROM SEAWEEDS in the sediment abandoned lower.
S70, by step S50 and step S60 supernatant liquor merge, be evaporated to relative density 1.1-1.2 and obtain concentrated solution II;
S80, in concentrated solution II, add ethanol to ethanol contend concentration 83-86%, after hold over night, abandon supernatant liquor, centrifugal after the washing with alcohol of precipitation percent by volume 74-76%;
S90, obtain SULFATED POLYSACCHARIDES FROM SEAWEEDS by carrying out spraying dry after the water dissolution of the precipitation 7-9 times amount after centrifugal.
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated.
Embodiment 1
1. SULFATED POLYSACCHARIDES FROM SEAWEEDS processing parameter
1.1 sea-tangles are selected and qualification
The place of production: north of China marine site.
Form: dry bundling.
Qualification: identified one by one by SULFATED POLYSACCHARIDES FROM SEAWEEDS quality standard by professional, rejects leaf Huang, leaf
The defective sea-tangle such as broken, mouldy.
1.2 soak operation
Feed intake: 200 kilograms of sea-tangles.
Immersion way: in four batches, often criticizes 50 kilograms and puts into stainless steel pond, makes sea-tangle unclamp rear all immersion up and down in 40 times of water gagings.
Soak time: 40 minutes.
1.3 cleanings and chopping operation
Cleaning way: rinse sea-tangle to clean with 40 times of water gagings.
Scavenging period: often criticize 10 minutes.
Chopping size: particle diameter 3 centimetres of large small shredss.
1.4 water extraction process
Get sea-tangle fragment and put into extractor, through successively three boilings extraction, add the water of 10 times amount, first time boils 2 hours, and second and third time respectively boils 1.5 hours at every turn.
1.5 concentrated one
Three extraction liquids concentrate on concentration tank to carry out first time and concentrates.
Mode: concentrating under reduced pressure.
Final concentration: relative density 1.10 (50 DEG C of heat are surveyed).
1.6 alcohol precipitations one
Concentrated solution adds ethanol to volumetric concentration 30%, carries out first time precipitation, hold over night.
1.7 centrifugal one
Collected after centrifugation supernatant liquor, and get sediment sample, carry out assay.[sediment detection] is undertaken by SULFATED POLYSACCHARIDES FROM SEAWEEDS quality standard.
1.8 sediments are heavy molten
If sediment SO
4 2-during >5%, with 30% ethanol molten sediment again, hold over night.
1.9 is centrifugal again
SO to sediment
4 2-during <5%, centrifugally remove sediment, collect supernatant liquor.
1.10 concentrated two
Centrifuged supernatant concentrates on concentration tank.
Mode: concentrating under reduced pressure.
Final concentration: relative density 1.17 (50 DEG C of heat are surveyed).
1.11 alcohol precipitation two
Concentrated solution adds ethanol to final concentration 85%, hold over night.
1.12 the precipitation of washing
Incline supernatant liquor, adds ethanol to volumetric concentration 75%, wash precipitation, hold over night.
1.13 centrifugal two
Get deposit sample after centrifugal and carry out assay.
1.14 spraying dry
After centrifugal, add 8 times amount water dissolution, spraying dry, obtains SULFATED POLYSACCHARIDES FROM SEAWEEDS.
Make total amount: 200 kilograms of sea-tangles obtain 5 ~ 6 kilograms of extracts.3 ~ 5 kilograms of dry powder are obtained after spray drying process.Yield 1.5 ~ 2.5%.The SULFATED POLYSACCHARIDES FROM SEAWEEDS monose composition gas chromatogram of gained is shown in Fig. 1.
The packaging of 1.15 SULFATED POLYSACCHARIDES FROM SEAWEEDS
For lucifuge, protection against the tide, the SULFATED POLYSACCHARIDES FROM SEAWEEDS after spraying dry answers aluminium-plastic sealing to pack.
2. quality examination
Determined by above-mentioned technical process, must be tested and appraised in process of production, sediment detection, initial survey, the quality inspection outpost of the tax office, Zhong Jian tetra-road, undertaken identifying and assay by SULFATED POLYSACCHARIDES FROM SEAWEEDS quality standard, this technique can guarantee the stable, controlled of quality product and consistence.
Sea-tangle in freshwater soaking process first stripping be non-efficient part iodine and N.F,USP MANNITOL, stripping substantially in 1 hour; Efficient part also can stripping quickly in neutral hot water, simultaneously also can a small amount of non-effective position algin of stripping.For this reason, we devise two step water extractions, and the reparation technology route of the safety molten with a step alcohol, science, succinct extraction, abstraction and purification analysed by two step alcohol.
Emphasis is presented below:
The determination of water logging extraction iodine and N.F,USP MANNITOL parameter
First by soaking and wash the process of sea-tangle, by non-effective position iodine and N.F,USP MANNITOL stripping.Select and produce 500 grams, complete stool sea-tangle with marine site, a collection of Yantai, 5 parts, every part of 3 samples, soak with the edible tap water of 40 times, soak the different time, within 20 minutes, change water once (10 minutes do not change water with 20 minutes persons), chopping (3 centimetres) after leaching, the condition extracting SULFATED POLYSACCHARIDES FROM SEAWEEDS is identical, with SO
4 2-content as the index of monitoring SULFATED POLYSACCHARIDES FROM SEAWEEDS quality, in table 1.
Table 1 kelp soaking washing time is on the impact (n=3) of SULFATED POLYSACCHARIDES FROM SEAWEEDS quality
After this, through test of many times, above-mentioned parameter is adjusted further, finally determine: soak 40 ~ 50 minutes for the edible clear water of Yantai, Shandong product complete stool sea-tangle with 30 ~ 40 times, water is changed once in centre.
Boiling water extraction
Laboratory test process choice normal pressure poach 6 hours, point four extractions, all add the water of 10 times amount, first time decocts 2 hours at every turn; Second time 1.5 hours; 1.5 hours third times; 4th time 1 hour.In pilot scale, decoct points for 3 times and within totally 5 hours, there is no obvious difference with dividing the quality product that decocts totally 6 hours for 4 times and productive rate.Finally we determine the extraction process extracted for 5 hours 3 times.
The technique (comprising SULFATED POLYSACCHARIDES FROM SEAWEEDS) of the boiling water extraction polysaccharide that the traditional technology of traditional Chinese medicine extraction and document are recorded, temperature condition 80 DEG C, 90 DEG C, 100 DEG C of decocting times are 4h, 6h, 8h.[see Du Bin, Chinese Pharmaceutical Journal 2002,37 volumes (9) p657].
Concentrating under reduced pressure parameter
Concentrating under reduced pressure, by triple effect concentrated (50 ~ 60 DEG C, 60 ~ 70 DEG C, 70 ~ 80 DEG C), starts alcohol precipitation to during relative density 1.10.
Alcohol precipitation parameter for the first time
Japan export to the west English one with 20% ethanol removing algin impurity, Anno (1966), Wang Zuoyun etc. then use the ethanol of 30%, we find all not contain effective constituent by the precipitation of the ethanol gained of two kinds of concentration, and the precipitation capacity that the ethanol of 30% is separated out wants many one times, increase the concentration to 35% of ethanol again, the amount of precipitation has increased slightly, and starts the phenomenon of the co-precipitation occurring SULFATED POLYSACCHARIDES FROM SEAWEEDS.The alcohol concn finally determining first time alcohol precipitation is 30%.
The concentrated parameter of second time
Condition is identical with first time, and for saving ethanol consumption, this is concentrated into relative density is 1.17.
Second time alcohol precipitation parameter
Extraction liquid starts when 65% alcohol concn to occur that SULFATED POLYSACCHARIDES FROM SEAWEEDS precipitates.For making SULFATED POLYSACCHARIDES FROM SEAWEEDS precipitate completely, the alcohol concn of employing 85% is determined in test, to obtain the thick product of the SULFATED POLYSACCHARIDES FROM SEAWEEDS of maximum, can save ethanol consumption again.
Purifying parameter
By the thick product SULFATED POLYSACCHARIDES FROM SEAWEEDS of the washing with alcohol of low concentration.Determine after test, by 75% washing with alcohol best results, in table 2.
Table 2 ethanol purification SULFATED POLYSACCHARIDES FROM SEAWEEDS is tested
The rate of transform
The total reducing sugar of the sea-tangle of scale up test and corresponding finished product SULFATED POLYSACCHARIDES FROM SEAWEEDS, total protein and sulfate content are detected, the results are shown in table 3.
Table 3 sea-tangle and SULFATED POLYSACCHARIDES FROM SEAWEEDS are containing surveying (%) result and the rate of transform (n=3)
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (10)
1. a preparation method for SULFATED POLYSACCHARIDES FROM SEAWEEDS, is characterized in that, comprises the following steps:
S10, sea-tangle be placed in himself quality 38-42 water doubly, soak 35-45 minute after, the water for himself quality 38-42 times amount is rinsed well;
S20, pre-treatment is carried out to sea-tangle, reject the defective sea-tangle that leaf is yellow, leaf is broken and mouldy, qualified sea-tangle is cut into the fragment of area 7-10 square centimeter;
S30, sea-tangle joined decoct three times in himself quality 9-11 water doubly, be respectively 1.9-2.1 hour, 1.4-1.6 hour, 1.4-1.6 hour;
S40, three decoction liquor are merged after be evaporated to relative density 1.05-1.15 and obtain concentrated solution I;
S50, in concentrated solution I, add ethanol to ethanol contend concentration 29-31%, centrifugal supernatant liquor and sediment after hold over night;
S60, sediment to be detected, if SO in sediment
4 2-mass percentage be greater than 5%, in sediment, add ethanol to ethanol contend concentration 29-31%, leave standstill centrifugal supernatant liquor to layering; If SO in sediment
4 2 -mass percentage be less than or equal to 5%, abandon sediment;
S70, by step S50 and step S60 supernatant liquor merge, be evaporated to relative density 1.1-1.2 and obtain concentrated solution II;
S80, in concentrated solution II, add ethanol to ethanol contend concentration 83-86%, after hold over night, abandon supernatant liquor, centrifugal after the washing with alcohol of precipitation percent by volume 74-76%;
S90, obtain SULFATED POLYSACCHARIDES FROM SEAWEEDS by carrying out spraying dry after the water dissolution of the precipitation 7-9 times amount after centrifugal.
2. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, be placed in by sea-tangle in step S10 in the water of himself quality 40 times and soak after 40 minutes, the water for himself quality 40 times amount is rinsed well.
3. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S20, qualified sea-tangle is cut into the fragment of area 9 square centimeters.
4. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S30, is joined by sea-tangle and decocts three times in the water of himself quality 10, be respectively 2 hours, 1.5 hours, 1.5 hours.
5. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S40, is evaporated to relative density 1.1 and obtains concentrated solution I after three decoction liquor being merged.
6. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S50, adds ethanol to ethanol contend concentration 30% in concentrated solution I, centrifugal supernatant liquor and sediment after hold over night.
7. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S60, if SO in sediment
4 2-mass percentage be greater than 5%, in sediment, add ethanol to ethanol contend concentration 30%, leave standstill centrifugal supernatant liquor to layering.
8. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S70, is merged by the supernatant liquor in step S50 and step S60, is evaporated to relative density 1.17 and obtains concentrated solution II.
9. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S80, ethanol is added to ethanol contend concentration 85% in concentrated solution II, after hold over night, abandon supernatant liquor, centrifugal after the washing with alcohol of precipitation percent by volume 75%.
10. the preparation method of SULFATED POLYSACCHARIDES FROM SEAWEEDS according to claim 1, is characterized in that, in step S90, the precipitation after centrifugal is obtained SULFATED POLYSACCHARIDES FROM SEAWEEDS with carrying out spraying dry after the water dissolution of 8 times amount.
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| CN201410328156.2A CN105273093B (en) | 2014-07-10 | 2014-07-10 | A kind of preparation method of seaweed polysaccharide sulfate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963325A (en) * | 2016-05-06 | 2016-09-28 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Application of sulfated polysaccharides from seaweed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279953A (en) * | 2000-08-04 | 2001-01-17 | 中国人民解放军军事医学科学院放射医学研究所 | Application of seeweed polyose sulphate |
| CN103239472A (en) * | 2013-05-10 | 2013-08-14 | 首都医科大学 | Novel medicinal application of fucoidan polysaccharide sulfate |
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2014
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279953A (en) * | 2000-08-04 | 2001-01-17 | 中国人民解放军军事医学科学院放射医学研究所 | Application of seeweed polyose sulphate |
| CN103239472A (en) * | 2013-05-10 | 2013-08-14 | 首都医科大学 | Novel medicinal application of fucoidan polysaccharide sulfate |
Non-Patent Citations (2)
| Title |
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| 徐怀德: "《药食同源新食品加工》", 31 July 2002 * |
| 李乃胜等: "《中国海洋水产品现代加工技术与质量安全》", 31 May 2010 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963325A (en) * | 2016-05-06 | 2016-09-28 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Application of sulfated polysaccharides from seaweed |
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