CN105267617A - Applications of ginseng-ophiopogon japonicus composition in preparation of anti-aging drugs - Google Patents
Applications of ginseng-ophiopogon japonicus composition in preparation of anti-aging drugs Download PDFInfo
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- CN105267617A CN105267617A CN201410244574.3A CN201410244574A CN105267617A CN 105267617 A CN105267617 A CN 105267617A CN 201410244574 A CN201410244574 A CN 201410244574A CN 105267617 A CN105267617 A CN 105267617A
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- ginseng
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- aging
- ophiopogon japonicus
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Abstract
The present invention belongs to the field of traditional Chinese medicines, and relates to news of a ginseng-ophiopogon japonicus composition in pharmacy, particularly to applications of a ginseng-ophiopogon japonicus composition in preparation of anti-aging drugs. Aging is a natural process and mainly comprises that the body is subjected to hypofunction, the homeostasis capacity and the stress ability decrease, and the cells, the tissues and the organs of the body present degeneration in the structures and the functions along with the age increase; and based on the hugeou old populations and the premature senescence populations, in order to fundamentally improve the life quality, the drug with effects of physical function improving and aging delaying is found so as to make the premature senescence population healthy age. The present invention provides the applications of the ginseng-ophiopogon japonicus composition in preparation of the anti-aging drugs. The animal experiment results show that the ginseng-ophiopogon japonicus composition can significantly prolong the lives of the normal populations and the premature senescence populations. The present invention provides the new uses of the ginseng-ophiopogon japonicus composition, wherein the ginseng-ophiopogon japonicus composition can be used for prevention of anti-aging drugs, and is expected to be developed into the new generation anti-aging drugs.
Description
Technical field
The present invention relates to Radix Ginseng, the novelty teabag of compositions Radix Ophiopogonis in pharmacy, be specifically related to Radix Ginseng, Radix Ophiopogonis compositions preparing the application in antiaging agent, belong to the field of Chinese medicines.
Background technology
Radix Ginseng be Araliaceae Radix Ginseng (
panaxginsengc.A.Mey) dry root and rhizome, main active is ginsenoside and ginseng polysaccharide.Radix Ginseng has strongly invigorating primordial QI, invigorating the spleen to benefit the lung, promoting the production of body fluid and inducing sedation of the mind Fructus Alpiniae Oxyphyllae, resisting fatigue, antitumor, shock, the effects such as blood fat reducing.
Radix Ophiopogonis be Liliaceae herbaceos perennial Radix Ophiopogonis (
ophiopogonjaponicus(Thumb) Ker-Gawl) dried root.Radix Ophiopogonis cold nature, sweet in the mouth, micro-hardship, has, have YIN nourishing benefit essence, Replenishing QI and nourishing YIN, clear away heart-fire the effect such as relieving restlessness, loosening bowel to relieve constipation, is one of conventional Chinese medicine.There is Radix Ophiopogonis pharmacological action widely, such as, resist myocardial ischemia, arrhythmia, defying age, antitumor, the effects such as immunomodulating.
Aging is the natural process of life, and mainly referring to increased with the age, and body function goes down, and homeostasis ability and stress ability decline, all degenerations that the cell of body, tissue, organ show on structure and fuction.Old and feeble result usually produces the disease relevant to aging, until dead.In the face of huge old group, senilism colony, fundamentally to improve their life quality, the method improving physical function, slow down aging will be found, enable them to healthy old and feeble.
But the function of most medicine and curative effect indefinite, the product life cycle is shorter, is wherein namely suitable for old people, and the medicine for prolog life being suitable for again senilism patient is then few, still can not meet the urgent health demand of this two classes crowd at present simultaneously.Aging is the complexity regulated and controled by body level of aggregation, multifactorial process, correspondingly requires that antiaging agent should be the adjustment of integral level to the effect of body.And the integrally-regulated effect of the too many levels that Chinese medicine compound has, Mutiple Targets, this is the antidotal characteristic of Chinese medicine and advantage just also, has huge potentiality in slow down aging.The present invention has broken away from the constraint of existing senile-resistant efficacy checking thinking, adopt nematode model directly demonstrate Radix Ginseng, Radix Ophiopogonis compositions there is antidotal biological activity, can apply preparing in antiaging agent.
Summary of the invention
The object of this invention is to provide Radix Ginseng, the novelty teabag of compositions Radix Ophiopogonis in pharmacy, be specifically related to Radix Ginseng, Radix Ophiopogonis compositions prepare the application in antiaging agent, to providing a kind of antiaging agent of clear efficacy.
Described Radix Ginseng, Radix Ophiopogonis compositions contain Radix Ginseng, Radix Ophiopogonis.
The mass ratio of the Radix Ginseng in described Radix Ginseng, Radix Ophiopogonis compositions, Radix Ophiopogonis, Fructus Schisandrae Chinensis is 9:9.
Radix Ginseng of the present invention, Radix Ophiopogonis compositions, its prescription ratio rationally can be changed according to Chinese prescription principle, and does not change this prescription and preparing the purposes in antiaging agent, and this it will be appreciated by those skilled in the art that.
Radix Ginseng of the present invention, Radix Ophiopogonis compositions preparing the application in antiaging agent, can by Radix Ginseng, Radix Ophiopogonis compositions add pharmaceutically acceptable carrier as filler, lubricant, wetting agent, penetration enhancement agent, absorption carrier, diluent, excipient etc., sweeting agent, flavouring agent etc. can be added if desired, be prepared into the multiple dosage form of the medicines such as injection, powder, granule, powder, pill, oral liquid, tablet, this it will be appreciated by those skilled in the art that.
Present invention employs Caenorhabditis elegans directly evaluates medicine activity of fighting against senium as model organism, Caenorhabditis elegans is used for defying age model and has the following advantages:
1. due to the gene of nematicide 60-80% and the related gene high conservative of the mankind, and the 17 bars pathways found so far, nematicide has wherein 12, and therefore the present invention utilizes Caenorhabditis elegans as the model organism of screening antiaging agent.Can utilize the genetic resources that nematicide is abundant, select suitable mutant according to research purpose, research aging and mechanism of resisting senility, several broad theories of Aging mechanism are all be proved to be in nematicide in fact.Therefore, nematicide is had to the medicine of anti-aging effects, be usually also considered to, to the mankind, also there is same effect.
2. Caenorhabditis elegans is used to durability analysis, so far the course of existing 30 years.Because of the advantage of its uniqueness, become the first-elected model of aging research.The nematicide generation cycle is short, is generally about 3 days; Life-span is short, is generally about 3 weeks.This makes the repetition stability of experiment become possibility.In order to ensure the accuracy of the reliability of experimental technique and experimental result, drug screening can obtain more accurately, believable information, it is necessary for repeating to test.
Because Caenorhabditis elegans has the advantage of above uniqueness, and become the first-elected model of aging research.Therefore nematicide can be used to the anti-aging effects evaluating compositions, and then judges that compositions is used to prepare the probability of antiaging agent.
The invention provides a kind of Radix Ginseng, Radix Ophiopogonis compositions preparing the novelty teabag in antiaging agent, Radix Ginseng, Radix Ophiopogonis compositions both can life-span of significant prolongation normal population, again can life-span of significant prolongation senilism colony, be expected to the antiaging agent being developed to a new generation.
Accompanying drawing explanation
Fig. 1 is the durability analysis figure of Chinese medicine compound of the present invention to mutant TK22 senilism nematicide aging effects.
Solid line wherein in figure is blank group, dotted line be Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group, half empty half solid line be Radix Ginseng, Radix Ophiopogonis compositions high dose medicament group.
Fig. 2 is the durability analysis figure of Chinese medicine compound of the present invention on the impact in normal type N2 nematicide life-span.
Solid line wherein in figure is blank group, dotted line be Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group.
Detailed description of the invention
Below provide specific embodiment with realize Radix Ginseng of the present invention, Radix Ophiopogonis compositions apply preparing in antiaging agent, but be not limited to these embodiments.
One, experiment material
1. bioorganism
Caenorhabditis elegans strain: normal type Caenorhabditis elegans N2, mutant senilism type Caenorhabditis elegans TK22 is purchased from CaenorhabditisGeneticsCenter(CGC).Caenorhabditis elegans N2 is normal type nematicide, for evaluating the anti-aging effects of medicine for normal population; Caenorhabditis elegans TK22 is senilism nematicide, life-span of this strain nematicide usually than normal strain nematicide few 15%-20%, TK22 for evaluating the defying age curative effect of medicine for failure model morning.
Bacterial strain: uracil leaky mutant escherichia coli OP50(
e.
coli), purchased from CaenorhabditisGeneticsCenter(CGC).
2. culture medium, medicine
NGM culture medium: NaCl1.5g, K
2hPO
41.3g, KH
2pO
48.5g, peptone 1.4g, agar powder 8.5g, distilled water 500mL.The 500 μ L cholesterol (5mg/ml, dehydrated alcohol is prepared) of filtration sterilization are added, 500 μ L1mol/LMgSO of high pressure steam sterilization after sterilizing
4, 500 μ L1mol/LCaCl
2.
M9 buffer: NaCl1.98g, Na
2hPO
42.38g, KH
2pO
41.20g, MgSO
40.048g, distilled water 400mL.
LB fluid medium: NaCl2g, peptone 2g, yeast extract 1g, distilled water 200mL.PH is regulated to be 7.0 with 1MNaOH after dissolving, high pressure steam sterilization.
Medicine:, Radix Ginseng, Radix Ophiopogonis medical material purchased from the large pharmacy of Lanzhou Hui Rentang.
3. instrument
High-pressure sterilizing pot (Shenan Medical Appliances Factory, Shanghai)
Single one side superclean bench (Suzhou Decontamination Equipment Plant)
Biochemical cultivation case (south of the River, Ningbo instrument plant)
Water isolation type constant incubator (Shanghai Yiheng Scientific Instruments Co., Ltd)
Zoom-stereo microscope (Shanghai Yiheng Scientific Instruments Co., Ltd)
Electronic balance (Beijing Sai Duolisi instrument system company limited)
Desk centrifuge (CT15E, Hitachi)
embodiment one Radix Ginseng, Radix Ophiopogonis composition water extract preparation
Radix Ginseng Rubra 9g, Radix Ophiopogonis 9g.First use distilled water immersion 20 minutes, then slow fire boils, and decocts 30min, and final volume is settled to 50mL, centrifugal, discards precipitation, by supernatant aseptic filtration, stand-by to 4 DEG C of refrigerators.
embodiment two Radix Ginseng, Radix Ophiopogonis compositions on the impact in mutant senilism type Caenorhabditis elegans TK22 life-span
1. Caenorhabditis elegans synchronization
By the adult M9 wash buffer of hatching of Caenorhabditis elegans in 1.5mL centrifuge tube, standing or centrifugal discarding please, 500 μ L1MNaOH and 500 μ L6.4%NaClO are added in each centrifuge tube, on vortex instrument, shake cracking be about 7min, the centrifugal 4min of 4000rpm, after sucking supernatant, the M9 of 500 μ L is added after rinsing 2 times with M9, after being placed in 20 DEG C of incubator hatchings, being inoculated in and being scribbled in the NGM solid culture ware of OP50, the Caenorhabditis elegans of synchronous growth can be obtained through 20 DEG C of cultivations.
N2, TK22 strain Caenorhabditis elegans that the present invention uses all adopts said method to carry out synchronization process.
2. containing Radix Ginseng, Radix Ophiopogonis compositions NGM plate preparation
The extracting solution of Chinese medicine compound ball embodiment one prepared is prepared by following method:
Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group: after 0.1mL stock solution is added 1.9mL distilled water, add in 18mLNGM, shake up, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices.
Radix Ginseng, Radix Ophiopogonis compositions high dose medicament group: after 0.2mL stock solution is added 1.8mL distilled water, add in 18mLNGM, shake up, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices.
Blank group: 2mL distilled water is added in 18mLNGM, shakes up, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices.
3. durability analysis experimental technique
By synchronized for L4 phase Caenorhabditis elegans, blank group is transferred to platinum filament hordeolum, Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group, in Radix Ginseng, Radix Ophiopogonis compositions high dose medicament group culture dish, often group three is parallel, and each parallel 25 Caenorhabditis elegans, are placed in 20 DEG C of cultivations, observe every day and count survival number and death toll, all dead to nematicide.Overlapping from generation to generation for preventing, be the floxuridine of 50 μMs containing concentration in NGM culture medium.The criterion of Caenorhabditis elegans death is touched with hordeolum, and nematicide is reactionless.Climb to culture dish edge or ware lid thirst, gets into nematicide inside agar and get rid of from experiment.
N2, TK22 strain Caenorhabditis elegans that the present invention uses all adopts said method to carry out durability analysis experiment.
4. result
Analysis of experimental data adopts SurvivalAnalysis process, the analysis of SPSS17.0 software.Result is as follows:
Fig. 1 is the durability analysis figure of Chinese medicine compound of the present invention to mutant TK22 senilism nematicide aging effects.
Solid line wherein in figure is blank group, dotted line be Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group, half empty half solid line be Radix Ginseng, Radix Ophiopogonis compositions high dose medicament group.
Radix Ginseng, low, the high dose group of compositions Radix Ophiopogonis all significant prolongation early life-spans (P<0.01) of failure model TK22 nematicide as can be seen from Figure 1.
The half survival time of TK22 nematicide and average life under the effect of table 1 Chinese medicine compound
| Group | Median survival time | Median survival time × 100/ blank median survival time (%) | Average life | Average life × 100/ blank average life (%) | P |
| Blank group | 15.97±0.62 | 15.07±0.55 | |||
| Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group | 22.34±0.59 | 139.87 | 21.25±0.63 | 141.04 | 0.000 |
| Radix Ginseng, Radix Ophiopogonis compositions high dose medicament group | 22.54±0.64 | 141.14 | 21.75±0.70 | 144.33 | 0.000 |
As can be drawn from Table 1, Radix Ginseng, Radix Ophiopogonis compositions significant prolongation early failure model TK22 nematicide life-span (
p<0.01).Under the optium concentration effect of this experiment, by morning, the average life of failure model TK22 nematicide extends 44.3%; By morning, the half survival natural law of failure model TK22 nematicide extends 41.1%.
Embodiment three Chinese medicine compound Radix Ginseng, Radix Ophiopogonis compositions on the impact in normal type N2 nematicide life-span
Fig. 2 is that Chinese medicine compound of the present invention affects durability analysis figure to the normal type N2 nematicide life-span.
Solid line wherein in figure is blank group, dotted line be Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group.
The half survival time of N2 nematicide and average life under the effect of table 2 Chinese medicine compound
| Group | Median survival time | Median survival time × 100/ blank median survival time (%) | Average life | Average life × 100/ blank average life (%) | P |
| Blank group | 19.04±0.40 | 100 | 18.19±0.41 | 100 | |
| Radix Ginseng, Radix Ophiopogonis compositions low-dose drugs group | 29.25±0.49 | 153.67 | 26.79±0.53 | 147.28 | 0.000 |
As can be seen from Fig. 2, table 2, Radix Ginseng, compositions significant prolongation life-span of normal type N2 nematicide Radix Ophiopogonis (
p<0.05), compare blank group and respectively the average life of normal type N2 nematicide is extended 47.3%, compare blank group and respectively the half of normal type N2 nematicide survival natural law is extended 53.7%.
As can be seen from the above embodiments, Radix Ginseng, Radix Ophiopogonis compositions not only can life-span of significant prolongation normal population, and can life-span of significant prolongation senilism colony, directly demonstrate Radix Ginseng, Radix Ophiopogonis compositions there is antidotal biological activity, can apply preparing in antiaging agent, be expected to the antiaging agent being developed to a new generation.
Claims (3)
1. Radix Ginseng, Radix Ophiopogonis compositions preparing the application in antiaging agent.
2. Radix Ginseng according to claim 1, Radix Ophiopogonis compositions preparing the application in antiaging agent, it is characterized in that Radix Ginseng, Radix Ophiopogonis compositions comprise Radix Ginseng, Radix Ophiopogonis.
3. Radix Ginseng according to claim 2, Radix Ophiopogonis compositions preparing the application in antiaging agent, it is characterized in that the Radix Ginseng contained by Radix Ginseng, Radix Ophiopogonis compositions, the mass ratio of Radix Ophiopogonis be 9:9.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410244574.3A CN105267617A (en) | 2014-06-04 | 2014-06-04 | Applications of ginseng-ophiopogon japonicus composition in preparation of anti-aging drugs |
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| CN201410244574.3A CN105267617A (en) | 2014-06-04 | 2014-06-04 | Applications of ginseng-ophiopogon japonicus composition in preparation of anti-aging drugs |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112957424A (en) * | 2021-03-09 | 2021-06-15 | 陕西省万寿制药有限责任公司 | Composition for improving physical performance of human body |
-
2014
- 2014-06-04 CN CN201410244574.3A patent/CN105267617A/en not_active Withdrawn
Non-Patent Citations (2)
| Title |
|---|
| 李培等: "《临床实用方剂手册》", 31 January 2003, 四川科学技术出版社 * |
| 邓钢等: "参麦液抗衰老的临床价值", 《铁道医学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112957424A (en) * | 2021-03-09 | 2021-06-15 | 陕西省万寿制药有限责任公司 | Composition for improving physical performance of human body |
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Application publication date: 20160127 |