CN105267239A - Memory-improving and calcium-enriching algae oil DHA and calcium mixed preparation product - Google Patents
Memory-improving and calcium-enriching algae oil DHA and calcium mixed preparation product Download PDFInfo
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- CN105267239A CN105267239A CN201410309890.4A CN201410309890A CN105267239A CN 105267239 A CN105267239 A CN 105267239A CN 201410309890 A CN201410309890 A CN 201410309890A CN 105267239 A CN105267239 A CN 105267239A
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- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 78
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- 239000004615 ingredient Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 47
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- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
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- FYDKNKUEBJQCCN-UHFFFAOYSA-N lanthanum(3+);trinitrate Chemical compound [La+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O FYDKNKUEBJQCCN-UHFFFAOYSA-N 0.000 description 1
- ICAKDTKJOYSXGC-UHFFFAOYSA-K lanthanum(iii) chloride Chemical compound Cl[La](Cl)Cl ICAKDTKJOYSXGC-UHFFFAOYSA-K 0.000 description 1
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Abstract
The invention relates to a memory-improving and calcium-enriching algae oil DHA (Docosahexaenoic Acid) and calcium mixed preparation product, which is prepared from the following ingredients in percentage by mass: 20 percent to 40 percent of algae oil DHA powder, 2 percent to 30 percent of milk calcium, 20 percent to 60 percent of milk powder and 4 percent to 25 percent of isomaltitol. The product solves the problem that the existing products only have a single function of the memory-improving function or the calcium-enriching function; and meanwhile, the constipation phenomenon caused by calcium-enriching product eating is relieved, so that the memory-improving and calcium-enriching algae oil DHA and calcium mixed preparation product is suitable for user groups simultaneously needing calcium enrichment and memory improvement to eat; the constipation phenomenon caused by calcium-enriching product eating is relieved; and the preparation product has good mouthfeel.
Description
Technical field
The present invention relates to field of health care products, particularly relate to the algae oil DHA of a kind of Improving memory and supplementary calcium and the mix preparation product of calcium.
Background technology
(1) algae oil DHA
DHA (docosahexenoic acid) is commonly called as NAOHUANGJIN, is a kind of to the very important highly unsaturated fatty acid of human body, belongs to the important member in Omega-3 unsaturated fatty acid family.Except the deposition of cholesterol in blood vessel wall can be stoped, prevent and alleviate outside the generation of atherosclerosis and coronary heart disease, DHA is also to the growth of nervous system cell with maintain very important, brain and amphiblestroid important composition composition, in human brain cortex, content is up to 20%, in eye retina, proportion reaches 50%, to fetus and infant intelligence and visual acuity most important.
Human body self can not synthesize DHA, must obtain from diet.DHA is mainly present in deep water ocean fishes, in some Sargassums, Lu Sheng fungus and seawater bacteria, also there is a large amount of DHA, and freshwater fish, only containing a small amount of DHA, does not generally contain or contain the DHA of seldom amount in common food.
The commercial source of DHA is ocean fishes, fish oil and algae oil.But fish oil poor stability, contains the organic pollution, heavy metal contaminants etc. of EPA (have effect for reducing blood fat, but have Developing restraint to grow to infant and promote the side effect of children's sexual precocity) and the marine pollution that can not remove completely.
Algae oil DNA is not containing EPA, higher, safer relative to the DHA purity extracted in traditional bathypelagic fish oil, is more suitable for anemia of pregnant woman and infants.
(2) calcium
Calcium is one of indispensable nutrient of human body, and people needed to replenish the calcium in each stage.
Investigation shows, the intake of Chinese town and country each age groups calcium is generally not enough, and Children and teenager is the key population of calcium deficiency.Chinese Academy Of Preventive Medicine Research Institute Of Nutrition And Food Hygiene carries out three national nutrition survey, and all show that in the diet nutrient of compatriots, calcium lacks the most, calcium intake on average only reaches 50% of quantity delivered every day.And calcium has important physiological action for human body, human body calcium deficiency may cause many diseases.Physician finds, calcium Deficiency of Intake and the calcium metabolism caused is unbalance, is cause the major reason calcium of the mankind's each age group generation various diseases to be the macroelement of needed by human, accounts for 2% of weight, wherein 99% be stored in skeleton and tooth, all the other 1% be present in blood and tissue in.Child's calcium deficiency will affect its normal development, and Gou building can be caused time serious sick.In, old people's calcium deficiency then can cause the various diseases such as osteoporosis, hyperosteogeny, colon cancer and hypertension.NI of China recommend calcium daily requirement be: 6 months to 10 years old for 600-800mg, 11-18 year be 1000-1200mg, within more than 18 years old, be 800mg to old age.
In existing product, the product of Improving memory is many, and the product of replenishing the calcium is quite a few, can Improving memory and the product of replenishing the calcium simultaneously but do not have.
A kind of health product of replenishing the calcium are disclosed in Chinese patent literature CN201110323119.9.A kind of health food for auxiliary improvement of memory is disclosed in Chinese patent literature CN200810028968.X.
But these two products of the prior art only have the effect of single Improving memory or calcium supplementing effect function respectively, cannot simultaneously Improving memory and replenishing the calcium.Therefore improper with the crowd replenished the calcium for needing Improving memory, there is limitation and inconvenience in the product consumption crowd in above patent application document and effect.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of can simultaneously Improving memory and the mix preparation product be made up of algae oil DHA and milk calcium of replenishing the calcium.Employ the algae oil DHA powder from deep-sea and genuine milk calcium powder simultaneously.
The algae oil DHA of Improving memory and supplementary calcium and a mix preparation product for calcium, comprise the algae oil DHA powder that mass percentage is 20%-40%, the milk calcium of 2%-30%, the milk powder of 20%-60%, the hydroxyl isomaltulose of 4%-25%.
Algae oil DHA powder mass percentage is 20%, and milk calcium mass percentage is 30%, and quality of milk powder percentage composition is 35%, and hydroxyl isomaltulose mass percentage is 15%.
Algae oil DHA powder mass percentage is 30%, and milk calcium mass percentage is 20%, and quality of milk powder percentage composition is 40%, and hydroxyl isomaltulose mass percentage is 10%.
Algae oil DHA powder mass percentage is 40%, and milk calcium mass percentage is 10%, and quality of milk powder percentage composition is 30%, and hydroxyl isomaltulose mass percentage is 20%.
Detailed description of the invention
Below the specific embodiment of the present invention is described in further detail; Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The algae oil DHA of Improving memory and supplementary calcium and a mix preparation product for calcium, comprise the algae oil DHA powder that mass percentage is 20%-40%, the milk calcium of 2%-30%, the milk powder of 20%-60%, the hydroxyl isomaltulose of 4%-25%.
Algae oil DHA powder mass percentage is 20%, and milk calcium mass percentage is 30%, and quality of milk powder percentage composition is 35%, and hydroxyl isomaltulose mass percentage is 15%.
Algae oil DHA powder mass percentage is 30%, and milk calcium mass percentage is 20%, and quality of milk powder percentage composition is 40%, and hydroxyl isomaltulose mass percentage is 10%.
Algae oil DHA powder mass percentage is 40%, and milk calcium mass percentage is 10%, and quality of milk powder percentage composition is 30%, and hydroxyl isomaltulose mass percentage is 20%.
Processing technology is as follows:
Sieve-weigh-mix-pack
1. sieve: each raw material is crossed 18 mesh sieves respectively;
2. weigh: each raw material after sieving is weighed by formula;
3. mix: load weighted each raw material is put into three-dimensional mixer, mixes 20 minutes;
4. pack: the material of mix homogeneously, in the machine that is packed in bag loading bag, namely obtain product.
Improving memory is tested
Human experimentation
1. materials and methods
1.1 samples: algae oil DHA milk calcium powder: laboratory is made by oneself;
1.2 test method
Carry out according to the human feeding trial code of auxiliary improving memory function in " health food inspection and assessment technical specification " (version in 2003), adopt Clinical Memory Test press the memory quotient of test request mensuration experimenter, main examiner person is through special training and obtain certificate.
1.3 test scale
Adopt " Clinical Memory Test " of Institute of Developed Organisms, Academia Sinica's revision in 1996.
The principle of 1.4 selection experimenters
(1) from the colony relatively more concentrated, each side influence factor is roughly the same, volunteer is selected; (2) schooling is basically identical; (3) belong to same year age group, if not at same year age group, then coping inventory divides and corrects; (4) similar test was not accepted; (5) article relevant with tested function are taken in eliminating in a short time, have influence on the judgement to result.
1.5 test-meal object and groupings
Concentrate and select study subject, implement informed consent.After first time memory quotient test being carried out to experimenter before taking given the test agent, by memory quotient (MQ), experimenter is divided into product group and matched group at random, considers educational level, age, sex etc. as far as possible, carry out harmony inspection.Number 105 people is selected in test-meal, and that complete full test-meal process is 101 people, wherein product group 51 people, matched group 50 people, rate 31.81% lost to follow-up.
1.6 test-meal methods
Matched group takes placebo, and product group takes product of the present invention, daily 2 times, each 1 bag.Take 1 month continuously.In accordance with double blinding principle in test-meal.
1.7 efficacy measures
Adopt Clinical Memory Test, with original point of each subtest after test, look into scale score, each subtest scale score is added to obtain total scale score, looks into memory quotient (MQ) with total scale score.
(1) memory scale score is pointed to; (2) learning by association scale score; (3) random shape re-recognizes scale score; (4) image is freed recall scale score; (5) scale score is recalled in the contact of portrait feature; (6) memory quotient.
1.8 results judge
Under the prerequisite of two groups of memory quotient equilibriums before the test, after test-meal, the memory quotient of product group is higher than matched group, and difference has statistical significance, memory quotient after product group test-meal is simultaneously higher than the memory quotient before its test-meal, and difference has statistical significance, can judge that product of the present invention has the effect of auxiliary improving memory function.
1.9 statistical procedures
Test data is measurement data, and two groups of each subtest scale scores and memory quotient t inspection are analyzed.Own control paired t-test, compare between group and check with two sample average volume t, the latter need carry out homogeneity test of variance, carries out suitable variable transitions to the data of nonnormal distribution or heterogeneity of variance, after meeting normal state or variance and be neat, carry out t inspection by the data transformed; If conversion data still can not meet normal state or the neat requirement of variance, use t inspection or rank test instead, but the data rank test that the coefficient of variation is too large.P < 0105 has statistical significance for difference.
2. result
The result of every subtest scale score before and after 2.1 test-meals
Two groups of every subtest scale score situations before and after table 1 test-meal
Table 1 is that before and after two groups of test-meals, each subtest scale score compares.Result shows, before test-meal, product group and matched group compare, the equal not statistically significant of every subtest scale score difference; After test-meal, product group points to memory, image subtest scale score of freeing recall all is significantly higher than matched group; Point to memory after the test-meal of product group, image frees recall, random shape re-recognizes before 3 subtest scale scores are significantly higher than test-meal; Pointing to the scale score pointing to memory before memory is significantly higher than test-meal after contrast test-meal, may, because being familiar with content measurement and method, the 2nd performance of the test being increased, the equal not statistically significant of all the other every subtest scale score differences.
Total scale proportion by subtraction is remembered comparatively for two groups before and after 2.2 food test-meals
Total scale score situation is remembered before and after table 2 liang group test-meal
From table 2, the total scale score of product group and matched group no significant difference before test-meal; After test-meal, product group is remembered total scale score and is significantly higher than matched group; Remember before total amount table score value is significantly higher than test-meal after the test-meal of product group, on average raise 14.16 points, and be significantly higher than matched group.Remember the distribution of total scale before and after the test-meal of product group and have statistical significance to comparing difference, and matched group no significant difference.
2.3 food are on the impact of experimenter's memory quotient
Memory quotient situation before and after table 3 liang group test-meal
From table 3, before test-meal, product group memory quotient compares with matched group, no significant difference; After test-meal, product group memory quotient is significantly higher than matched group; Before after the test-meal of product group, memory quotient is significantly higher than test-meal, average rising 13.64 points, is significantly higher than matched group; Before and after the test-meal of product group, memory quotient paired comparison difference has statistical significance, and matched group no significant difference.
Prove that this invention product has the action and efficacy of Improving memory by above-mentioned human experimentation.
To replenish the calcium zoopery
1. materials and methods
1.1 materials and reagent
Algae oil DHA milk calcium powder: laboratory is made by oneself;
Nitric acid, perchloric acid, hydrochloric acid, Lanthanum (III) nitrate, above reagent is the pure commercially available reagent of domestic top grade;
Low calcium feedstuff.
1.2 laboratory animal
One-level Kunming mouse 40, female, body weight 18-22g.
1.3 key instrument equipment
TAS-986G atomic absorption spectrophotometer, TU-1800 ultraviolet-uisible spectrophotometer, Beijing Puxi General Instrument Co., Ltd; SX5-12 chamber type electric resistance furnace, Shanghai Shen Guang instrument and meter company limited; AL-104 type precise electronic balance, Mettler-Toledo Instrument (Shanghai) Co., Ltd..
1.4 animal experiment method
40 mices conform raising 1 week, are divided into 2 groups at random: low calcium matched group, product group, often organize 10.Often organize and freely drink deionized water, equal ad lib normal feedstuff every day.Product group gavage every day, matched group gives equal-volume deionized water gavage, continuous 30 days.
From experiment, every day carries out normal observation, comprises animal appearance, sign, behavioral activity etc., weighs in 1 time every 1 day, according to Mouse Weight change adjustment gavage amount, and observes the impact on body weight.
After testing 3 weeks, animal is moved in rustless steel metabolic cage, carry out the calcium metabolism experiment of 3d, collect the 72 hours feces often organizing mice, and record food-intake and the body weight change of 3 days.
Last, to fasting after tested material 24 hours, plucks eyeball blood sampling, and disconnected neck puts to death mice, gets liver, femur, carries out indices mensuration.
1.5 assay method
1.5.1 calcium content
After femur, feedstuff, feces etc. being dried to constant weight, blood to be measured, liver, femur, feedstuff, feces etc. are placed in chamber type electric resistance furnace, ashing 6 hours at 600 DEG C.If individual sample ashing is not thorough, then add the little fire heating on adjustable electric furnace of 1 milliliter of mixed acid, repeated multiple times until digestion completely, lets cool.Blank assay should be done during sample digestion simultaneously, digest under the same conditions.Fully dissolved by the hydrochloric acid that ash after digestion adds 2%, be transferred in volumetric flask with dropper by sample Digestive system, repeatedly wash crucible on a small quantity with water, cleaning mixture is incorporated in volumetric flask, with 0.5% lanthanum chloride solution dilution, and standardize solution.Calcium content is recorded with atomic absorption spectrophotometry.
1.5.2 femur weight and length
Put to death mice, get its bilateral femur, pick clean its muscle, tendon and connective tissue, after being dried to constant weight, the slide gauge (precision is 0.02mm) using Shanghai Measuring and Cutting Tools Plant to produce measures femur length, and AL-104 type precise electronic balance (precision 0.1mg) claims its femur weight.
1.6 statistical procedures
All data are all processed by SPSS11.0 software.Group difference carries out significance test by variance analysis, and compares between two with least significant difference (LSD method) inspection.
2. experimental result
The change of calcium content in 2.1 blood, liver and femur
Experimental group affects result to calcium content in Mice Body: in table 4.
Calcium content in table 4 blood, liver and femur
| Group | Blood sample (g/g) | Liver (μ g/g) | Femur (mg/g) |
| Matched group | 77.41±4.86 | 22.49±2.46 | 245.12±9.28 |
| Product group | 79.69±6.28 | 23.49±2.11 | 295.68±12.54 |
Note: have statistical significance (variance analysis, F=3.482, P < 0.05) with matched group comparing difference
Tested material product group compares with matched group, and the content difference of whole blood calcium, liver calcium does not have statistical significance; Tested material product group significantly improves the calcium content of femur, has statistical significance (variance analysis, F=3.482, P < 0.05) with matched group comparing difference.
Can prove that product of the present invention has the action and efficacy of replenishing the calcium by above-mentioned zoopery.
Improve the experiment because taking in the constipation side effect that a large amount of calcium causes
1. materials and methods
1.1 materials and reagent
Algae oil DHA milk calcium powder, self-control milk calcium powder: laboratory is made by oneself;
1.2 test-meal object and groupings
Number 86 people is selected in test-meal, and that complete full test-meal process is 86 people, wherein product group 43 people, matched group 43 people.
1.3 test-meal methods
Matched group takes self-control milk calcium powder, and product group takes product of the present invention, daily 2 times, each 1 bag.Take 1 month continuously.In accordance with double blinding principle in test-meal.
1.4 constipation symptom scorings
Within 1st day, rise record integration carried out to following symptom after after test-meal, infrequent defecation natural law, feces character, difficult defecation or not to the utmost, abdominal distention etc. 5, refer to following table
Table 5 symptom scores
| Project | Difficult defecation | Defecation not to the utmost | Interval time (d) | Abdominal distention | Stool a |
| 0 point | Nothing | Nothing | 1～2 | Nothing | 4-7 type |
| 1 point | Once in a while | Once in a while | 3 | Once in a while | 3 types |
| 2 points | Frequent > 25% b | Obvious > 25% b | 4～5 | Obvious > 25% b | 2 types |
| 3 points | Each > 50% b | Very obviously > 50% b | Be greater than 5 | Very obviously > 50% b | 1 type |
Note:
afor Brisiol typing,
bshared by a certain order of severity time referring to constipation clinical symptoms whole observing time percentage ratio.
1.5 statistical procedures
Statistical analysis adopts SPSS13.0 software, enumeration data with
represent, adopt the inspection of t value, P < 0.05 difference has statistical significance.
2. result
2.1 clinical symptoms integral contrasts
Table 6 clinical symptoms integral contrast
| Group | Abdominal distention | Difficult defecation | Stool | Defecation not to the utmost | Interval natural law |
| Product group | |||||
| Before test-meal | 1.41±0.85 | 2.26±0.71 | 2.13±0.56 | 1.76±0.85 | 2.65±0.62 |
| After test-meal | 0.63±0.43 | 0.95±0.46 | 0.84±0.45 | 0.76±0.42 | 1.07±0.31 |
| Matched group | |||||
| Before test-meal | 1.43±0.75 | 2.25±0.69 | 2.12±0.54 | 1.79±0.83 | 2.63±0.61 |
| After test-meal | 0.96±0.61 | 1.25±0.53 | 1.16±0.57 | 1.07±0.62 | 1.41±0.47 |
Product group improves significantly (P < 0.05) for the constipation phenomenon caused because taking in calcium complement agent.Can prove that product of the present invention has the action and efficacy improving the constipation phenomenon that edible calcium supplement cause by above-mentioned experiment.
Comprehensive above human body and zoopery, the double effects that product of the present invention has Improving memory and replenishes the calcium, can improve the constipation phenomenon taken in calcium complement agent and cause simultaneously, have good mouthfeel.
The present invention can be applicable to multiple crowds such as middle-aged and elderly people, anemia of pregnant woman, children and adolescents for Improving memory and the needs of replenishing the calcium.
In China, the intake of calcium comparatively lacks, and the crowd in each stage is improved the needs of memory simultaneously, and therefore product of the present invention has wide consumer groups and the market space.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvement and modification, these improve and modification also should be considered as protection scope of the present invention.
Claims (5)
1. the algae oil DHA of an Improving memory and supplementary calcium and the mix preparation product of calcium, comprise the algae oil DHA powder that mass percentage is 20%-40%, mass percentage is the milk calcium of 2%-30%, mass percentage is the milk powder of 20%-60%, and mass percentage is the hydroxyl isomaltulose of 4%-25%.
2. the algae oil DHA of Improving memory according to claim 1 and supplementary calcium and the mix preparation product of calcium, it is characterized in that: described algae oil DHA powder mass percentage is 20%, described milk calcium mass percentage is 30%, described quality of milk powder percentage composition is 35%, and described hydroxyl isomaltulose mass percentage is 15%.
3. the algae oil DHA of Improving memory according to claim 1 and supplementary calcium and the mix preparation product of calcium, it is characterized in that: described algae oil DHA powder mass percentage is 30%, described milk calcium mass percentage is 20%, described quality of milk powder percentage composition is 40%, and described hydroxyl isomaltulose mass percentage is 10%.
4. the algae oil DHA of Improving memory according to claim 1 and supplementary calcium and the mix preparation product of calcium, it is characterized in that: described algae oil DHA powder mass percentage is 40%, described milk calcium mass percentage is 10%, described quality of milk powder percentage composition is 30%, and described hydroxyl isomaltulose mass percentage is 20%.
5. the algae oil DHA of Improving memory according to claim 1 and supplementary calcium and the mix preparation product of calcium, is characterized in that: the processing technology of the algae oil DHA of described Improving memory and supplementary calcium and the mix preparation product of calcium divides following four steps:
(1) sieve: each raw material is crossed 18 mesh sieves respectively;
(2) weigh: each raw material after sieving is weighed by formula;
(3) mix: load weighted each raw material is put into three-dimensional mixer, mixes 20 minutes;
(4) pack: the material of mix homogeneously, in the machine that is packed in bag loading bag, namely obtain product.
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Cited By (2)
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| CN105770799A (en) * | 2016-03-09 | 2016-07-20 | 宁波君瑞生物科技有限公司 | Granular preparation with brain memory improving function |
| CN115380956A (en) * | 2022-08-25 | 2022-11-25 | 北京斯利安健康科技有限公司 | Composition for assisting in improving memory and preparation method thereof |
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| WO2007108827A1 (en) * | 2006-03-23 | 2007-09-27 | Nestec S.A. | High-calorie nutritional supplement |
| CN102885310A (en) * | 2012-10-19 | 2013-01-23 | 中科乐仁（北京）科技发展有限公司 | Health care food composition capable of assisting in improving memory, and preparation method thereof |
| CN103202483A (en) * | 2012-01-11 | 2013-07-17 | 浙江康恩贝健康产品有限公司 | Nutritional composition for calcium supplement |
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| WO2007108827A1 (en) * | 2006-03-23 | 2007-09-27 | Nestec S.A. | High-calorie nutritional supplement |
| CN103202483A (en) * | 2012-01-11 | 2013-07-17 | 浙江康恩贝健康产品有限公司 | Nutritional composition for calcium supplement |
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| CN105770799A (en) * | 2016-03-09 | 2016-07-20 | 宁波君瑞生物科技有限公司 | Granular preparation with brain memory improving function |
| CN115380956A (en) * | 2022-08-25 | 2022-11-25 | 北京斯利安健康科技有限公司 | Composition for assisting in improving memory and preparation method thereof |
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