CN105266163A - Preparation method of comprehensive bacillus natto enzyme stock solution - Google Patents

Preparation method of comprehensive bacillus natto enzyme stock solution Download PDF

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CN105266163A
CN105266163A CN201510689681.1A CN201510689681A CN105266163A CN 105266163 A CN105266163 A CN 105266163A CN 201510689681 A CN201510689681 A CN 201510689681A CN 105266163 A CN105266163 A CN 105266163A
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vitamin
bacillus natto
preparation
comprehensive
enzyme stoste
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CN105266163B (en
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张淑莲
赵廷远
陈旅平
包选平
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Total Heart Dalian Trading Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention discloses a preparation method of a comprehensive bacillus natto enzyme stock solution. Preferably, plant raw materials are pulped, sugar and collagen powder are added, a fermentation broth is prepared, then a bacillus natto seed solution is added, the mixture is fermented for 50-100 days at the temperature of 32-38 DEG C, a supernatant of the fermentation broth is taken, nutritional substances including vitamins, isoflavone, amino acid and the like are added, the mixture is left to stand for 3-6 months at the temperature of 8-14 DEG C, centrifugation is performed for 5-10 min at the rotation speed of 400-1000 rpm, the supernatant is taken and put in a low-temperature evaporation thickener, and the comprehensive bacillus natto enzyme stock solution is prepared. The comprehensive bacillus natto enzyme stock solution prepared with the method has high content of the nutritional substances and high thrombolysis capacity and is a good raw material substance for preparation of anti-thrombus medicines and anti-thrombus health-care products. The preparation method is simple and does not require complex separation and purification equipment, and the prepared enzyme stock solution has high nattokinase enzyme activity.

Description

A kind of preparation method of bacillus natto comprehensive enzyme stoste
Technical field
The present invention relates to the zymotic fluid of a kind of Bacillus natto, particularly a kind of preparation method of bacillus natto comprehensive enzyme stoste.
Background technology
Natto Pseudomonas bacillus subtilis Bacillus natto subspecies, be the bacillus of a class aerobic, the degeneration-resistant sporozoite of interior life, self is not pathogenic, only there is cell monolayer adventitia, can multiple antibiotic and enzyme be produced, there is broad spectrum antibiotic activity and extremely strong anti-adversity ability, in soybean isoflavone by natto strain, a kind of serine protease that can be produced by Bacillus natto, Nattokinase by name, the plasmase of Direct Resolution thrombus, has powerful thrombolytic effect.
But, the Nattokinase content that natural fermented soybean produces is low, be difficult to prepare the thrombolytic drug becoming and there is treatment function, therefore, current fermenting bacillus natto generally adopts and the animal-plant material with health care carries out fermenting and producing health products, as, patent 201210008190.2 discloses the production method of a kind of Bacillus natto and Cordyceps Militaris composite enzyme series of products; Patent 201010136617.8 discloses the production method of bacillus natto comprehensive enzyme low-molecular beverage; Patent 201310598684.5 discloses the technical research that fermenting bacillus natto method produces low molecule cherry ferment beverage; Patent 201310604713.4 discloses the method for fermenting bacillus natto legal system for low molecule ginsenoside peptide; Patent 201310617333.4 discloses the method for a kind of fermenting bacillus natto legal system for low molecule sea cucumber peptide.The product prepared in above-mentioned patent is health products, have certain effect, but result of use is not obvious, and nutritional labeling depends on special animal-plant material, and preparation cost is high.
Summary of the invention
The present invention is to overcome above-mentioned defect of the prior art, by the preparation method selecting superior strain and optimization for fermentation technology to propose a kind of bacillus natto comprehensive enzyme stoste.
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get plant material 80-100 part, add the making beating of 80-180 part water, then add sugared 1-2 part, collagen protein powder 1-2 part, makes zymotic fluid;
(2) Bacillus natto seed liquor is added according to the mass percent of zymotic fluid 2-4%, 32-38 DEG C of condition bottom fermentation 50-100 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.01-0.2 part vitamin, 0.2-0.5 part isoflavones, 0.3-0.6 part amino acid;
(4) leave standstill 3-6 month under being placed in 8-14 DEG C of condition, under 400-1000rpm condition, centrifugal 5-10min, gets supernatant, is placed in the 1/2-1/8 that low-temperature evaporation thickener is concentrated into centrifugate volume, makes.
Described Bacillus natto is bacillus subtilis strain QR, and its preserving number is CGMCCNo.11274.The depositary institution of this bacterial classification: China Committee for Culture Collection of Microorganisms's common micro-organisms center; Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date: on August 25th, 2015; Classification And Nomenclature: bacillus subtilis BacillusSubtilis.
Described plant material is soybean, peanut, corn, pumpkin, tomato, potato, apple, pears, red bean, cowpea, sesame, grape, pineapple, apple, one or more in Chinese cabbage.
Described vitamin is vitamin A, vitamin C, vitamin E, vitamin D, vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin B12, nicotinic acid, one or more in vitamin K.
Described isoflavones is isoflavones.
Described amino acid is alanine, valine, leucine, isoleucine, methionine, aspartic acid, glutamic acid, lysine, arginine, glycine, serine, threonine, cysteine, asparagine, glutamine, phenylalanine, tyrosine, histidine, tryptophan, one or more in proline.
The operating temperature of described low-temperature evaporation thickener is 4-12 DEG C, and vacuum is-70kpa ~-95kpa.
Compared with prior art, the present invention has following beneficial effect: bacillus natto comprehensive enzyme stoste prepared by method of the present invention, nutrition content is high, containing activated low molecular peptide, and oligosaccharides, saponin(e, the composition that isoflavones etc. are useful to human body, meanwhile, the Nattokinase enzymatic activity contained is high, thrombolysis ability is strong, is to prepare antithrombotic reagent and the good raw material of health products thereof.Method preparation of the present invention is simple, without the need to the separation and purification equipment of complexity, prepares the ferment stoste that high Nattokinase enzyme is lived.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, but is to be understood that protection scope of the present invention not by the restriction of detailed description of the invention.
embodiment 1
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get soybean 90 parts, add 130 parts of water making beating, then add sugar 1 part, collagen protein powder 1 part, makes zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 3%, 37 DEG C of condition bottom fermentations 80 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.12 part of vitamin, 0.4 part of isoflavones, 0.32 part of amino acid;
Described vitamin is vitamin A 0.01 part, vitamin C 0.02 part, vitamin E 0.01 part, vitamin D 0.01 part, vitaminB10 .01 part, vitamin B2 0.01 part, vitamin B5 0.01 part, vitamin B6 0.01 part, vitamin B12 0.01 part, nicotinic acid 0.01 part, vitamin K 0.01 part.
Described amino acid is alanine 0.01 part, valine 0.01 part, leucine 0.02 part, isoleucine 0.02 part, methionine 0.05 part, aspartic acid 0.03 part, 0.01 part, glutamic acid, lysine 0.01 part, arginine 0.03 part, glycine 0.02 part, serine 0.01 part, threonine 0.01 part, cysteine 0.01 part, asparagine 0.01 part, glutamine 0.05 part, phenylalanine 0.01 part, 0.02 part, tyrosine, histidine 0.01 part, tryptophan 0.01 part, proline 0.01 part.
(4) leave standstill 4 months under being placed in 10 DEG C of conditions, under 800rpm condition, centrifugal 6min, gets supernatant, is placed in low-temperature evaporation thickener, is 8 DEG C in operating temperature, and vacuum is under the condition of-80kpa, is concentrated into 1/4 of centrifugate volume, makes.
Bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains more active low-molecular peptide, oligosaccharides, saponin(e, isoflavones.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 3000FU.
embodiment 2
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get peanut 20 parts, corn 30 parts, 40 parts, pumpkin, add 80 parts of water making beating, then add sugar 1 part, collagen protein powder 1 part, makes zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 2%, 35 DEG C of condition bottom fermentations 90 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.16 part of vitamin, 0.2 part of isoflavones, 0.4 part of amino acid;
Described vitamin is vitamin A 0.01 part, vitamin C 0.05 part, vitamin E 0.01 part, vitamin D 0.01 part, vitaminB10 .01 part, vitamin B2 0.01 part, vitamin B5 0.01 part, vitamin B6 0.01 part, vitamin B12 0.04 part.
Described amino acid is alanine 0.02 part, valine 0.02 part, leucine 0.02 part, isoleucine 0.02 part, methionine 0.05 part, aspartic acid 0.03 part, 0.01 part, glutamic acid, lysine 0.01 part, arginine 0.03 part, glycine 0.02 part, serine 0.01 part, threonine 0.01 part, cysteine 0.01 part, asparagine 0.01 part, glutamine 0.05 part, phenylalanine 0.01 part, 0.02 part, tyrosine, histidine 0.01 part, tryptophan 0.01 part, proline 0.03 part.
(4) leave standstill 3 months under being placed in 8 DEG C of conditions, under 400rpm condition, centrifugal 5min, gets supernatant, is placed in low-temperature evaporation thickener, is 4 DEG C in operating temperature, and vacuum is under the condition of-95kpa, is concentrated into 1/2 of centrifugate volume, makes.
Bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains more active low-molecular peptide, oligosaccharides, saponin(e, isoflavones.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 2800FU.
embodiment 3
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get tomato 30 parts, 30 parts, potato, apple 30 parts, add 100 parts of water making beating, then add sugar 2 parts, collagen protein powder 2 parts, makes zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 4%, 38 DEG C of condition bottom fermentations 50 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.015 part of vitamin, 0.5 part of isoflavones, 0.42 part of amino acid;
Described vitamin is vitamin A 0.01 part, vitamin C 0.05 part, vitamin E 0.01 part, vitamin D 0.01 part, vitaminB10 .01 part, vitamin B2 0.01 part, vitamin B5 0.01 part, vitamin B6 0.01 part, vitamin B12 0.03 part.
Described amino acid is alanine 0.02 part, valine 0.02 part, leucine 0.02 part, isoleucine 0.02 part, methionine 0.05 part, aspartic acid 0.03 part, 0.01 part, glutamic acid, lysine 0.01 part, arginine 0.03 part, glycine 0.04 part, serine 0.01 part, threonine 0.01 part, cysteine 0.01 part, asparagine 0.01 part, glutamine 0.05 part, phenylalanine 0.01 part, 0.02 part, tyrosine, histidine 0.01 part, tryptophan 0.01 part, proline 0.03 part.
(4) leave standstill 3 months under being placed in 14 DEG C of conditions, under 1000rpm condition, centrifugal 10min, gets supernatant, is placed in low-temperature evaporation thickener, is 4 DEG C in operating temperature, and vacuum is under the condition of-80kpa, is concentrated into 1/6 of centrifugate volume, makes.
Bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains more active low-molecular peptide, oligosaccharides, saponin(e, isoflavones.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 3200FU.
embodiment 4
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get 35 parts, red bean, cowpea 35 parts, sesame 20 parts, add 180 parts of water making beating, then add sugar 2 parts, collagen protein powder 2 parts, Lavender 1 part, makes zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 3%, 36 DEG C of condition bottom fermentations 90 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.12 part of vitamin, 0.4 part of isoflavones, 0.32 part of amino acid;
Described vitamin is vitamin A 0.01 part, vitamin C 0.02 part, vitamin E 0.01 part, vitamin D 0.01 part, vitaminB10 .01 part, vitamin B2 0.01 part, vitamin B5 0.01 part, vitamin B6 0.01 part, vitamin B12 0.01 part, nicotinic acid 0.01 part, vitamin K 0.01 part.
Described amino acid is alanine 0.01 part, valine 0.01 part, leucine 0.02 part, isoleucine 0.02 part, methionine 0.05 part, aspartic acid 0.03 part, 0.01 part, glutamic acid, lysine 0.01 part, arginine 0.03 part, glycine 0.02 part, serine 0.01 part, threonine 0.01 part, cysteine 0.01 part, asparagine 0.01 part, glutamine 0.05 part, phenylalanine 0.01 part, 0.02 part, tyrosine, histidine 0.01 part, tryptophan 0.01 part, proline 0.01 part.
(4) leave standstill 5 months under being placed in 9 DEG C of conditions, under 900rpm condition, centrifugal 8min, gets supernatant, is placed in low-temperature evaporation thickener, is 4 DEG C in operating temperature, and vacuum is under the condition of-80kpa, is concentrated into 1/4 of centrifugate volume, makes.
Bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains more active low-molecular peptide, oligosaccharides, saponin(e, isoflavones.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 4000FU.
embodiment 5
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get soybean 90 parts, add 130 parts of water making beating, then add sugar 1 part, collagen protein powder 1 part, Rosa Damascana 1 part, makes zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 3%, 37 DEG C of condition bottom fermentations 80 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.12 part of vitamin, 0.4 part of isoflavones, 0.32 part of amino acid;
Described vitamin is vitamin A 0.01 part, vitamin C 0.02 part, vitamin E 0.01 part, vitamin D 0.01 part, vitaminB10 .01 part, vitamin B2 0.01 part, vitamin B5 0.01 part, vitamin B6 0.01 part, vitamin B12 0.01 part, nicotinic acid 0.01 part, vitamin K 0.01 part.
Described amino acid is alanine 0.01 part, valine 0.01 part, leucine 0.02 part, isoleucine 0.02 part, methionine 0.05 part, aspartic acid 0.03 part, 0.01 part, glutamic acid, lysine 0.01 part, arginine 0.03 part, glycine 0.02 part, serine 0.01 part, threonine 0.01 part, cysteine 0.01 part, asparagine 0.01 part, glutamine 0.05 part, phenylalanine 0.01 part, 0.02 part, tyrosine, histidine 0.01 part, tryptophan 0.01 part, proline 0.01 part.
(4) leave standstill 4 months under being placed in 10 DEG C of conditions, under 800rpm condition, centrifugal 6min, gets supernatant, is placed in low-temperature evaporation thickener, is 8 DEG C in operating temperature, and vacuum is under the condition of-80kpa, is concentrated into 1/4 of centrifugate volume, makes.
Bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains more active low-molecular peptide, oligosaccharides, saponin(e, isoflavones.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 4000FU.
Comparative example 1
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get soybean 90 parts, add 130 parts of water making beating, then add sugar 1 part, make zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 3%, 37 DEG C of condition bottom fermentations 80 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.12 part of vitamin, 0.4 part of isoflavones, 0.32 part of amino acid;
Described vitamin is vitamin A 0.01 part, vitamin C 0.02 part, vitamin E 0.01 part, vitamin D 0.01 part, vitaminB10 .01 part, vitamin B2 0.01 part, vitamin B5 0.01 part, vitamin B6 0.01 part, vitamin B12 0.01 part, nicotinic acid 0.01 part, vitamin K 0.01 part.
Described amino acid is alanine 0.01 part, valine 0.01 part, leucine 0.02 part, isoleucine 0.02 part, methionine 0.05 part, aspartic acid 0.03 part, 0.01 part, glutamic acid, lysine 0.01 part, arginine 0.03 part, glycine 0.02 part, serine 0.01 part, threonine 0.01 part, cysteine 0.01 part, asparagine 0.01 part, glutamine 0.05 part, phenylalanine 0.01 part, 0.02 part, tyrosine, histidine 0.01 part, tryptophan 0.01 part, proline 0.01 part.
(4) leave standstill 4 months under being placed in 10 DEG C of conditions, under 800rpm condition, centrifugal 6min, gets supernatant, is placed in low-temperature evaporation thickener, is 8 DEG C in operating temperature, and vacuum is under the condition of-80kpa, is concentrated into 1/4 of centrifugate volume, makes.
Bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains more active low-molecular peptide, oligosaccharides, saponin(e, isoflavones.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 2200FU.
Comparative example 2
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get soybean 90 parts, add 130 parts of water making beating, then add sugar 1 part, collagen protein powder 1 part, makes zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 3%, 37 DEG C of condition bottom fermentations 80 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.12 part of vitamin, 0.4 part of isoflavones, 0.32 part of amino acid;
Described vitamin is vitamin A 0.01 part, vitamin C 0.02 part, vitamin E 0.01 part, vitamin D 0.01 part, vitaminB10 .01 part, vitamin B2 0.01 part, vitamin B5 0.01 part, vitamin B6 0.01 part, vitamin B12 0.01 part, nicotinic acid 0.01 part, vitamin K 0.01 part.
Described amino acid is alanine 0.01 part, valine 0.01 part, leucine 0.02 part, isoleucine 0.02 part, methionine 0.05 part, aspartic acid 0.03 part, 0.01 part, glutamic acid, lysine 0.01 part, arginine 0.03 part, glycine 0.02 part, serine 0.01 part, threonine 0.01 part, cysteine 0.01 part, asparagine 0.01 part, glutamine 0.05 part, phenylalanine 0.01 part, 0.02 part, tyrosine, histidine 0.01 part, tryptophan 0.01 part, proline 0.01 part.
(4) leave standstill 4 months under being placed in 10 DEG C of conditions, getting supernatant, be placed in low-temperature evaporation thickener, is 8 DEG C in operating temperature, and vacuum is under the condition of-80kpa, is concentrated into 1/4 of stoste volume, makes.
Bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains more active low-molecular peptide, oligosaccharides, saponin(e, isoflavones.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 2000FU.
comparative example 3
A preparation method for bacillus natto comprehensive enzyme stoste, carries out in accordance with the following steps:
(1) according to parts by weight, get soybean 90 parts, add 130 parts of water making beating, then add sugar 1 part, collagen protein powder 1 part, makes zymotic fluid;
(2) Bacillus natto seed liquor (bacillus subtilis strain QR, its preserving number is CGMCCNo.11274) is added according to the mass percent of zymotic fluid 3%, 37 DEG C of condition bottom fermentations 80 days;
(3) get fermented liquid supernatant liquid, under being placed in 10 DEG C of conditions, leave standstill 4 months, centrifugal 6min under 800rpm condition, getting supernatant, be placed in low-temperature evaporation thickener, is 8 DEG C in operating temperature, vacuum is under the condition of-80kpa, is concentrated into 1/4 of centrifugate volume, makes.
The active low-molecular peptide that bacillus natto comprehensive enzyme stoste prepared by the present embodiment contains, oligosaccharides, saponin(e, isoflavones is less.Adopt traditional handicraft, above-mentioned bacillus natto comprehensive enzyme stoste low temperature evaporate to dryness is added 25wt% pharmaceutically acceptable carrier, makes granula amount, every 0.5g, containing Nattokinase 2000FU.
Be only specific embodiments of the invention above, but the present invention is not limited thereto, the changes that any person skilled in the art can think of all should fall into protection scope of the present invention.

Claims (7)

1. a preparation method for bacillus natto comprehensive enzyme stoste, is characterized in that, carries out in accordance with the following steps:
(1) according to parts by weight, get plant material 80-100 part, add the making beating of 80-180 part water, then add sugared 1-2 part, collagen protein powder 1-2 part, makes zymotic fluid;
(2) Bacillus natto seed liquor is added according to the mass percent of zymotic fluid 2-4%, 32-38 DEG C of condition bottom fermentation 50-100 days;
(3) get fermented liquid supernatant liquid, according to parts by weight, add 0.01-0.2 part vitamin, 0.2-0.5 part isoflavones, 0.3-0.6 part amino acid;
(4) leave standstill 3-6 month under being placed in 8-14 DEG C of condition, under 400-1000rpm condition, centrifugal 5-10min, gets supernatant, is placed in the 1/2-1/8 that low-temperature evaporation thickener is concentrated into centrifugate volume, makes.
2. the preparation method of bacillus natto comprehensive enzyme stoste according to claim 1, is characterized in that, described Bacillus natto is bacillus subtilis strain QR, and its preserving number is CGMCCNo.11274.
3. the preparation method of bacillus natto comprehensive enzyme stoste according to claim 1, is characterized in that, described plant material is soybean, peanut, corn, pumpkin, tomato, potato, apple, pears, red bean, cowpea, sesame, grape, pineapple, apple, one or more in Chinese cabbage.
4. the preparation method of bacillus natto comprehensive enzyme stoste according to claim 1, is characterized in that, described vitamin is vitamin A, vitamin C, vitamin E, vitamin D, vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin B12, nicotinic acid, one or more in vitamin K.
5. the preparation method of bacillus natto comprehensive enzyme stoste according to claim 1, is characterized in that, described isoflavones is isoflavones.
6. the preparation method of bacillus natto comprehensive enzyme stoste according to claim 1, is characterized in that, described amino acid is alanine, valine, leucine, isoleucine, methionine, aspartic acid, glutamic acid, lysine, arginine, glycine, serine, threonine, cysteine, asparagine, glutamine, phenylalanine, tyrosine, histidine, tryptophan, one or more in proline.
7. the preparation method of bacillus natto comprehensive enzyme stoste according to claim 1, is characterized in that, the operating temperature of described low-temperature evaporation thickener is 4-12 DEG C, and vacuum is-70kpa ~-95kpa.
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CN105146402A (en) * 2015-09-09 2015-12-16 安徽管仲宫神生物科技有限公司 Preparation method of purple sweet potato enzyme powder

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