CN105255953A - Method for pre-processing corn stalks through physical-chemical-biological method - Google Patents
Method for pre-processing corn stalks through physical-chemical-biological method Download PDFInfo
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- CN105255953A CN105255953A CN201510647321.5A CN201510647321A CN105255953A CN 105255953 A CN105255953 A CN 105255953A CN 201510647321 A CN201510647321 A CN 201510647321A CN 105255953 A CN105255953 A CN 105255953A
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- viride
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention relates to a method for pre-processing corn stalks through a physical-chemical-biological method. The corn stalks are firstly smashed and physically processed, then the corn stalks are chemically processed through dilute alkali, and finally, the corn stalks are biologically processed through phanerochaete chrysosporium and cellulose degradation bacteria. Lignin removal is facilitated due to dilute alkali processing, the corn stalks can be fully degraded easily because of mixed fermentation, the utilizing rate is increased, and microorganisms producing ethyl alcohol are inoculated into the pre-processed stalks to produce ethyl alcohol, or feeding yeast is inoculated to produce protein feed. The method is simple in technology and convenient to implement.
Description
Technical field
The present invention relates to the pretreatment process of stalk, be specifically related to the method for a kind of physical-chemical-Biological Pretreatment maize straw, belong to technological field of biochemistry.
Background technology
Stalk is a kind of very abundant renewable energy source, forms primarily of Mierocrystalline cellulose, hemicellulose and xylogen.Wherein xylogen is a kind of polymer aromatics, can not be hydrolyzed saccharogenesis, and Mierocrystalline cellulose and hemicellulose is all the source of potential fermentable sugars.By straw saccharification (enzymic hydrolysis), be converted into fermentable sugars, produce fuel alcohol, single cell protein and organic acid by fermentable and be considered to one of effective way utilizing straw, but the mutual weave in of Mierocrystalline cellulose, hemicellulose and xylogen forms cell wall structure in stalk, and cell is sticked together, the degraded of any element of the first species of this structures shape be interweaved will inevitably be subject to the restriction, particularly xylogen of other composition to cellulosic coating function.In addition, be joined together to form the fibrous bundle of crystalline structure between cellulose macromolecule by a large amount of hydrogen bonds, this special construction makes grape sugar chain in order and piles up closely, defines the structure of highly crystalline.Therefore these features make many cellulose-decomposing bacterium degrading straw inefficiencies.So carrying out effective pre-treatment to stalk is an of paramount importance link during stalk resource utilizes.Common pretreatment process is physics pre-treatment (such as machinery shatters, grind, steam explosion, radiotreatment, Microwave Pretreatment, ultrasonic pretreatment etc.), Chemical Pretreatment (diluted acid, diluted alkaline, ammoniacal liquor etc.), Biological Pretreatment (composite fungus agent, white-rot fungi, enzyme etc.) and combination pre-treatment (as ultrasonic wave and alkaline process combined pretreatment etc.).Because each treatment process has certain defect, such as chemistry, Physical process have environmental pollution, problem that energy consumption is high, and the Biochemical method cycle is long, and efficiency is low.
Summary of the invention
The object of the invention is: the method that a kind of physical-chemical-Biological Pretreatment maize straw is provided, adopt compounding technology and physics, chemistry and biological method used in combination, make up respective deficiency, play maximum effect.
Technical solution of the present invention is that the method for this pre-treatment maize straw comprises the following steps:
(1) physical treatment: maize straw is first pulverized by pulverizer, crosses 40 mesh sieves after pulverizing;
(2) chemical treatment: the maize straw after pulverizing with the NaOH solution process of mass concentration 1.0%, solid-to-liquid ratio is 1:10,100 DEG C of 1h, and filter, filter residue is washed to neutrality, dries to constant weight, as fermentation substrate;
(3) carry out a biological disposal upon: first prepare every 10mL containing FeSO
47H
2o0.0005g, MnSO
47H
2o0.0016g, ZnSO
47H
2o0.0014g, CoCl
2the micro-mixed solution of 0.002g; Next prepares nutrient solution, and in nutrient solution, the mass percent concentration of each component is: (NH
4)
2sO
41%, KH
2pO
40.3%, MgSO
40.05%, CaCl
20.05%, all the other are water; Then prepare mixed solution, mixed solution obtains by adding micro-mixed solution 1mL in every 100mL nutrient solution; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C of sterilizing 20min, obtain solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10%-14% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, after 28 DEG C of cultivation 3-5d, obtains pretreatment product.
Wherein, cellulose-degrading bacteria be aspergillus niger, viride and
trichodermaasperelloidesin one or more; Several mould blending ratio is respectively: aspergillus niger: viride=1:1, aspergillus niger:
trichodermaasperelloides=1:1, viride:
trichodermaasperelloides=1:1, aspergillus niger: viride:
trichodermaasperelloides=1:1:1, aspergillus niger: viride:
trichodermaasperelloides=2:1:1, aspergillus niger: viride:
trichodermaasperelloides=1:2:1, aspergillus niger: viride:
trichodermaasperelloides=1:1:2.
Wherein, described aspergillus niger and viride adopt PDA solid medium to activate, the preparation process of PDA substratum is: take 200g potato after potato is cleaned peeling and be cut into small pieces, add water well-done, then use eight layers of filtered through gauze, add 15g agar, continue heated and stirred mixing, after agar has dissolved, add 20g glucose, stir, slightly supply moisture again to 1000mL after cooling.
Wherein, described
trichodermaasperelloidesadopt wort solid medium to activate, the preparation process of wort solid medium is: take some amount Fructus Hordei Germinatus, pulverizes, add 4 times to the water of Fructus Hordei Germinatus amount, 55-60 DEG C of insulation saccharification, constantly stir, Glycation extend iodometric titration, after 3-4h, by saccharified liquid 4-6 layer filtered through gauze; Filtered liquid Ovum Gallus domesticus album is clarified, and method is the about 20mL that added water by an egg albumen, mixes well to generation foam, then pours in saccharified liquid and stir, refilter after boiling, obtain the wort of clarification; Thin up becomes the wort of 5-6 ° of B é; Add 16g agar in wort, 0.1MPa pressure, sterilizing 15min, obtain wort solid medium.
Wherein, described Phanerochaete chrysosporium utilizes comprehensive PDA solid medium to activate, and the preparation process of comprehensive PDA culture medium is: on the basis of 1LPDA solid medium, add KH
2pO
43g, MgSO
41.5g, vitamins B
10.001g.
Wherein, the preparation method of involved microbial spore suspension is: the bacterial strain sterilized water activated is washed spore, be placed in the triangular flask containing granulated glass sphere, at 28 DEG C of 120r/min shaking table vibration 20min, vibration liquid is filtered with 4 layers of sterilizing lens wiping paper, with blood counting chamber, filtrate miospore is counted under the microscope, adjust its concentration to 10
6individual/mL, is kept at 4 DEG C of refrigerators for subsequent use.
Advantage of the present invention is:
1, the degradation rate that the present invention adopts diluted alkaline pre-treatment, Phanerochaete chrysosporium fermentative processing greatly improves xylogen in stalk.
2, the present invention have studied the impact of mixed fungus fermentation on xylogen, hemicellulose and cellulose degradation rate in stalk, find to adopt mixed fungus fermentation degraded crop material, different microorganisms secretes complementary enzyme in degraded, in metabolism, make stalk fully be degraded, thus improve the degradation rate of stalk cellulose.
3, the present invention be compare different pretreatments method to stalk in xylogen, hemicellulose, screen after the impact of content of cellulose change, diluted alkaline process is adopted to remove partial lignin, efficient degradation microorganism is utilized to carry out Biological Pretreatment to stalk, xylogen in stalk after pre-treatment, hemicellulose and cellulosic degradation rate reach 95% respectively, more than 75% and 35%, what greatly improve stalk can utilising efficiency, required equipment is simple, simple process, separately or wheat bran can be equipped with through this pretreated maize straw, bean dregs, access producing and ethanol microorganisms producing ethanol or access fodder yeast is utilized to produce protein fodder etc.Solve agricultural crop straw and be difficult to the difficult problem recycled of degrading, very there is meaning.
Accompanying drawing explanation
Fig. 1 is the impact of different pretreatments method on xylogen, Mierocrystalline cellulose and hemicellulose level change in maize straw.
Fig. 2 is the impact that the combination of different cellulose-degrading bacteria and inoculative proportion change xylogen, Mierocrystalline cellulose and hemicellulose level in maize straw.
Fig. 3 is the impact of cellulose-degrading bacteria inoculum size on xylogen, Mierocrystalline cellulose and hemicellulose level change in maize straw.
Fig. 4 is that cellulose-degrading bacteria fermentation number of days is on the impact of xylogen, Mierocrystalline cellulose and hemicellulose level change in maize straw.
Embodiment
Further illustrate technical solution of the present invention below in conjunction with specific embodiment, these embodiments can not be interpreted as it is restriction to technical scheme.
Embodiment 1: according to following steps pre-treatment maize straw
(1) maize straw is first pulverized by pulverizer, crosses 40 mesh sieves after pulverizing;
(2) maize straw after pulverizing with the NaOH solution process of mass concentration 1.0%, solid-to-liquid ratio is 1:10,100 DEG C of 1h, and filter, filter residue is washed to neutrality, dries to constant weight, as fermentation substrate;
(3) every 10mL is first prepared containing FeSO
47H
2o0.0005g, MnSO
47H
2o0.0016g, ZnSO
47H
2o0.0014g, CoCl
2.0.002g micro-mixed solution; Next prepares nutrient solution, and in nutrient solution, the mass percent concentration of each component is: (NH
4)
2sO
41%, KH
2pO
40.3%, MgSO
40.05%, CaCl
20.05%, all the other are water; Then prepare mixed solution, mixed solution obtains by adding micro-mixed solution 1mL in every 100mL nutrient solution; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the aspergillus niger spore suspension of individual/mL, cultivates 3d, obtains pretreatment product for 28 DEG C.
As shown in Fig. 1 the 8th group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 98.7%, 81.6% and 43.2%.
Embodiment 2: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the viride spore suspension of individual/mL, cultivates 4d, obtains pretreatment product for 28 DEG C.
As shown in Fig. 2 the 1st group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 98.1%, 77.1% and 41.6%.
Embodiment 3: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6individual/mL's
trichodermaasperelloidesspore suspension, cultivates 4d, obtains pretreatment product for 28 DEG C.
As shown in Fig. 2 the 2nd group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 98.8%, 81.7% and 44.2%.
Embodiment 4: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 4d, obtains pretreatment product for 28 DEG C; Wherein, in cellulose-degrading bacteria spore suspension, aspergillus niger, viride two bacterium inoculative proportion are 1:1.
As shown in Fig. 2 the 3rd group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 99.1%, 78.1% and 45.9%.
Embodiment 5: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 4d, obtains pretreatment product for 28 DEG C; Wherein, aspergillus niger in cellulose-degrading bacteria spore suspension,
trichodermaasperelloidestwo bacterium inoculative proportions are 1:1.
As shown in Fig. 2 the 4th group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 98.7%, 81.3% and 47.8%.
Embodiment 6: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 4d, obtains pretreatment product for 28 DEG C; Wherein, cellulose-degrading bacteria spore suspension Green wood mould,
trichodermaasperelloidestwo bacterium inoculative proportions are 1:1.
As shown in Fig. 2 the 5th group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 99.3%, 81.4% and 47.5%.
Embodiment 7: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 4d for 28 DEG C, obtains the pretreatment product produced for protein fodder; Wherein, aspergillus niger in cellulose-degrading bacteria spore suspension, viride,
trichodermaasperelloidesthree bacterium inoculative proportions are 1:1:1.
As shown in Fig. 2 the 6th group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 98.6%, 82.5% and 49.9%.
Embodiment 8: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 4d for 28 DEG C, obtains the pretreatment product produced for protein fodder; Wherein, aspergillus niger in cellulose-degrading bacteria spore suspension, viride,
trichodermaasperelloidesthree bacterium inoculative proportions are 2:1:1.
As shown in Fig. 2 the 7th group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 98.2%, 81.9% and 48.7%.
Embodiment 9: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 4d for 28 DEG C, obtains the pretreatment product produced for protein fodder; Wherein, aspergillus niger in cellulose-degrading bacteria spore suspension, viride,
trichodermaasperelloidesthree bacterium inoculative proportions are 1:2:1.
As shown in Fig. 2 the 8th group, now in stalk, xylogen, hemicellulose and cellulosic degradation rate are respectively 99.2%, 83.8% and 48.1%.
Embodiment 10: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 12% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 4d for 28 DEG C, obtains the pretreatment product produced for protein fodder; Wherein, aspergillus niger in cellulose-degrading bacteria spore suspension, viride,
trichodermaasperelloidesthree bacterium inoculative proportions are 1:1:2.
As shown in Figure 3, now xylogen, hemicellulose and cellulosic degradation rate are respectively 98%, 84.3% and 53.2% in stalk.
Embodiment 11: step (1), (2) are with embodiment 1; The preparation of step (3) mixed solution is with embodiment 1; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C, sterilizing 20min, obtains solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 14% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 5d for 28 DEG C, obtains the pretreatment product produced for protein fodder; Wherein, aspergillus niger in cellulose-degrading bacteria spore suspension, viride,
trichodermaasperelloidesthree bacterium inoculative proportions are 1:1:2.
As shown in Figure 4, now xylogen, hemicellulose and cellulosic degradation rate are respectively 98.6%, 86.7% and 58.3% in stalk.
Fig. 1 is the impact of different pretreatments method on xylogen, Mierocrystalline cellulose and hemicellulose level change in maize straw, 1 represents pulverizing+diluted acid group, 2 represent pulverizing+diluted alkaline group, 3 represent pulverizing+ammoniacal liquor group, 4 represent pulverizing+microwave+diluted alkaline group, 5 represent pulverizing+microwave+ammoniacal liquor group, 6 represent pulverizing+whiterot fungi+aspergillus niger group, 7 represent pulverizing+diluted acid+whiterot fungi+aspergillus niger group, 8 represent pulverizing+diluted alkaline+whiterot fungi+aspergillus niger group, 9 represent pulverizing+ammoniacal liquor+whiterot fungi+aspergillus niger group, 10 represent pulverizing+microwave+diluted alkaline+whiterot fungi+aspergillus niger group, 11 represent pulverizing+microwave+ammoniacal liquor+whiterot fungi+aspergillus niger group, the reason that final selection is the 8th group, although the cellulose degradation rate of the 10th group is higher than the 8th group, but the 10th group of cellulosic degraded some occur in pulverizing+microwave+diluted alkaline pretreatment stage, and the degraded of this stage fiber element cannot provide carbon source for yeast, because after physical-chemical pre-treatment, need to filter, filter residue is washed to neutrality, dry to constant weight just as fermentation substrate.Therefore compare, the pretreating scheme of the 8th group is better.
Fig. 2 is the impact that the combination of different cellulose-degrading bacteria and inoculative proportion change xylogen, Mierocrystalline cellulose and hemicellulose level in maize straw; Experimental group 1 is viride, and experimental group 2 is
trichodermaasperelloides, experimental group 3 is aspergillus niger: viride=1:1, and experimental group 4 is aspergillus niger:
trichodermaasperelloides=1:1, experimental group 5 is viride:
trichodermaasperelloides=1:1, experimental group 6 is aspergillus niger: viride:
trichodermaasperelloides=1:1:1, experimental group 7 is aspergillus niger: viride:
trichodermaasperelloides=2:1:1, experimental group 8 is aspergillus niger: viride:
trichodermaasperelloides=1:2:1, experimental group 9 is aspergillus niger: viride:
trichodermaasperelloides=1:1:2.
Fig. 3 is the impact of cellulose-degrading bacteria inoculum size on xylogen, Mierocrystalline cellulose and hemicellulose level change in maize straw.
Fig. 4 cellulose-degrading bacteria fermentation number of days is on the impact of xylogen, Mierocrystalline cellulose and hemicellulose level change in maize straw.
Claims (6)
1. the method for physical-chemical-Biological Pretreatment maize straw, is characterized in that the method for this pre-treatment maize straw comprises the following steps:
(1) physical treatment: maize straw is first pulverized by pulverizer, crosses 40 mesh sieves after pulverizing;
(2) chemical treatment: the maize straw after pulverizing with the NaOH solution process of mass concentration 1.0%, solid-to-liquid ratio is 1:10,100 DEG C of 1h, and filter, filter residue is washed to neutrality, dries to constant weight, as fermentation substrate;
(3) carry out a biological disposal upon: first prepare every 10mL containing FeSO
47H
2o0.0005g, MnSO
47H
2o0.0016g, ZnSO
47H
2o0.0014g, CoCl
2the micro-mixed solution of 0.002g; Next prepares nutrient solution, and in nutrient solution, the mass percent concentration of each component is: (NH
4)
2sO
41%, KH
2pO
40.3%, MgSO
40.05%, CaCl
20.05%, all the other are distilled water; Then prepare mixed solution, mixed solution obtains by adding micro-mixed solution 1mL in every 100mL nutrient solution; In the fermentation substrate of step (2) according to solid-liquid ratio be 1:2.25 with mixed solution, regulate pH5.5, at 121 DEG C of sterilizing 20min, obtain solid-state fermentation culture medium; To be mixed with bacteria concentration is 10
6the Phanerochaete chrysosporium spore suspension of individual/mL is with in the inoculum size of quality 10% access solid-state fermentation culture medium, and ferment 10d; Again with the inoculum size of quality 10%-14% access 10
6the cellulose-degrading bacteria spore suspension of individual/mL, cultivates 3-5d, obtains pretreatment product for 28 DEG C.
2. the method for physical-chemical according to claim 1-Biological Pretreatment maize straw, is characterized in that: cellulose-degrading bacteria be aspergillus niger, viride and
trichodermaasperelloidesin one or more; Several mould blending ratio is respectively: aspergillus niger: viride=1:1, aspergillus niger:
trichodermaasperelloides=1:1, viride:
trichodermaasperelloides=1:1, aspergillus niger: viride:
trichodermaasperelloides=1:1:1, aspergillus niger: viride:
trichodermaasperelloides=2:1:1, aspergillus niger: viride:
trichodermaasperelloides=1:2:1, aspergillus niger: viride:
trichodermaasperelloides=1:1:2.
3. the method for physical-chemical according to claim 2-Biological Pretreatment maize straw, it is characterized in that: described aspergillus niger and viride adopt PDA solid medium to activate, the preparation process of PDA substratum is: take 200g potato after potato is cleaned peeling and be cut into small pieces, add water well-done, then use eight layers of filtered through gauze, add 15g agar, continue heated and stirred mixing, after agar has dissolved, add 20g glucose, stir, slightly supply moisture again to 1000mL after cooling.
4. the method for physical-chemical according to claim 2-Biological Pretreatment maize straw, is characterized in that: described
trichodermaasperelloidesadopt wort solid medium to activate, the preparation process of wort solid medium is: take some amount Fructus Hordei Germinatus, pulverizes, add 4 times to the water of Fructus Hordei Germinatus amount, 55-60 DEG C of insulation saccharification, constantly stirs, Glycation extend iodometric titration, after 3-4h, by saccharified liquid 4-6 layer filtered through gauze; Filtered liquid Ovum Gallus domesticus album is clarified, and method is the about 20mL that added water by an egg albumen, mixes well to generation foam, then pours in saccharified liquid and stir, refilter after boiling, obtain the wort of clarification; Thin up becomes the wort of 5-6 ° of B é; Add 16g agar in wort, 0.1MPa pressure, sterilizing 15min, obtain wort solid medium.
5. the method for physical-chemical according to claim 1-Biological Pretreatment maize straw, it is characterized in that: described Phanerochaete chrysosporium utilizes comprehensive PDA solid medium to activate, and the preparation process of comprehensive PDA culture medium is: on the basis of 1LPDA solid medium, add KH
2pO
43g, MgSO
41.5g, vitamins B
10.001g.
6. the method for the physical-chemical according to claim 3,4 or 5-Biological Pretreatment maize straw, it is characterized in that the preparation method of involved microbial spore suspension is: the bacterial strain sterilized water activated is washed spore, be placed in the triangular flask containing granulated glass sphere, at 28 DEG C of 120r/min shaking table vibration 20min, vibration liquid is filtered with 4 layers of sterilizing lens wiping paper, with blood counting chamber, filtrate miospore is counted under the microscope, adjust its concentration to 10
6individual/mL, is kept at 4 DEG C of refrigerators for subsequent use.
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| CN107034255A (en) * | 2017-06-16 | 2017-08-11 | 中南大学 | A kind of method of utilization lignin-degrading bacteria reinforcing abandoned biomass sodium carbonate pretreatment |
| CN107058427A (en) * | 2017-06-16 | 2017-08-18 | 中南大学 | A kind of method of utilization lignin-degrading bacteria reinforcing abandoned biomass ammonia process pretreatment |
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