CN105255796B - A kind of azotobacter MBC5 and its application - Google Patents
A kind of azotobacter MBC5 and its application Download PDFInfo
- Publication number
- CN105255796B CN105255796B CN201510859663.3A CN201510859663A CN105255796B CN 105255796 B CN105255796 B CN 105255796B CN 201510859663 A CN201510859663 A CN 201510859663A CN 105255796 B CN105255796 B CN 105255796B
- Authority
- CN
- China
- Prior art keywords
- microbial inoculum
- azotobacter
- mbc5
- level
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of azotobacter (Massilia albidiflava) MBC5, its deposit number is CGMCC No.10821, the deposit date is on Mays 19th, 2015, and depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center.The bacterial strain can still live and reproduce under arid, the big temperature difference, saline and alkaline disaster and strong ultraviolet radiation and have nitrogenase activity.Its manufactured microbial inoculum can be applied to the ecosystem of arid, the big temperature difference, saline and alkaline disaster and high ultraviolet radiation, and the nitrogen accumulation of these ecosystems can be increased, it can play the role of improveing soil environment, improve the physiological activity of microorganism and plant, increase net primary productivity.
Description
Technical field
The invention belongs to biological nitrogen fixation fields, and in particular to a kind of azotobacter MBC5 and its application.
Background technique
Desert ecosystem accounts for 41% (Delgado-Baquerizo, et al., 2013) of global area, net primary
Productivity accounts for about 20% (Whittaker, 1975) of terrestrial ecosystem productivity.In desert ecosystem, Soil Nitrogen
The scarcity of attainment point seriously limit system net primary productivity (Asner, et al., 2003, Crutsinger, et al.,
2013, Reich, et al., 2006).And desertification is also further developing, and results in land deterioration, and nitrogen nutrient is lost
Seriously.
Nitrogen input mode in natural environment is mainly biological nitrogen fixation.Nitrogen fixation, which increases soil, can utilize nitrogen content,
Supplementing nitrogen for microorganism and plant promotes it to grow and be metabolized, to increase the primary productivity of the ecosystem, and for life
The Nitrogen Cycling of state system also has vital effect.Biological nitrogen fixation mainly has symbiotic nitrogen fixation and growing nitrogen-fixing.Due to famine
Unconcerned area surroundings inclement condition, vegetation is rare, therefore the leguminous plant that can carry out symbiotic nitrogen fixation is also very rare, growing nitrogen-fixing
It is the main nitrogen input mode of desert ecosystem.Therefore, growing nitrogen-fixing is for desertification control work and desert ecosystem
The recovery of system is of great importance.
In order to help desertification control to work, the research and application for desert soil azotobacter are extremely urgent.Mesh
The preceding report for azotobacteria is considerably less for the report of azotobacter still based on symbiotic nitrogen-fixing bacteria.And these are few
It measures known growing nitrogen-fixing microbial inoculum to require the environmental conditions such as environment temperature and humidity, resistance is poor, is applied to agriculture more
In field or compost, it is unsuitable for the desert ecosystem of harsh environmental conditions.
It is therefore desirable to provide a kind of resistance it is strong, suitable for the fixed nitrogen with authigenic nitrogen fixation capacity of desert ecosystem
Bacterial strain.
Summary of the invention
The purpose of the present invention is to provide one kind be suitable for arid, the temperature difference is big, saline and alkaline disaster and high uitraviolet intensity and
It can be with the bacterial strain of growing nitrogen-fixing, to increase soil nitrogenase activity and effective nitrogen content.
To achieve the above objectives, the present invention provides a kind of azotobacter (Massilia albidiflava) MBC5,
Its deposit number is CGMCC No.10821, and the deposit date is on May 19th, 2015, depositary institution was Chinese microorganism strain preservation
Administration committee's common micro-organisms center.
Above-mentioned bacterial strains are Massilia albidiflava.Bacterial strain Massilia albidiflava (strain name)-MBC5
(bacterial strain name) is that the topsoil sterile device of Desert Area acquisition cryogenic conditions are being taken back laboratory, by separation, pure
Culture, Gram's staining, nitrogenase activity detection and 16srDNA sequencing gained.
Morphological character: the bacterial strain is observed in Glanz stained slice sees that cell is not spiral in single, straight bacillus
Shape.Atrichia does not generate sheath or protrusion, without rest stage, does not form brood cell, no pod membrane, Glanz feminine gender.
Cultural character: the bacterial strain on Ah 's shellfish culture medium after 28 DEG C of -30 DEG C of culture 48h, well-grown, obligate need
Oxygen.Colonial morphology is different, most diameter 1cm, and round, protrusion, edge are irregular.
Physiological property: NaCl (50g/L, 30 DEG C) growth experiment sample, pyrrolidinyl side's amine enzyme test sample, sxemiquantitative touching
Enzyme tests sample, and oxidase negative, indoles is negative, and string test is negative, nitrate reduction negative, urease negative.
Functional characteristic: it by inclined-plane Ah 's shellfish culture medium culture, is measured with acetylene reduction method, which has gone out fixed nitrogen
Activity.
The present invention also provides the microbial inoculums for containing the azotobacter MBC5.The microbial inoculum can be liquid bacterial agent,
It can be solid fungicide.
Optionally, in the microbial inoculum also contain thallus adsorbent material, the thallus adsorbent material be selected from turf, perlite,
At least one of vermiculite power and micro mist calcium carbonate.
Optionally, in the microbial inoculum, the content of azotobacter MBC5 is 2.1 × 108-2.9×108cfu/g。
Preferably, in the microbial inoculum, the content of azotobacter MBC5 is 2.9 × 108cfu/g。
Optionally, the pH value of the microbial inoculum is 6.8-7.2.
The present invention also provides the preparation methods of the microbial inoculum, comprising the following steps:
The azotobacter MBC5 is inoculated in primary inclined plane culture medium, 28-30 DEG C culture 3-5 days, obtain one
Grade bacterial strain；By the level-one strain inoculated in secondary liquid culture medium, shake culture 2-3 days under the conditions of 28-30 DEG C is obtained
Second level bacterial strain；By the second level strain inoculated in three-level solid medium, cultivates under the conditions of 28-30 DEG C and obtained after 1-2 days
Obtain three-level bacterial strain；By the three-level bacterial strain and turf according to (2.1 × 108-2.9×108) cfu/g proportion mix it is even after obtain
Microbial inoculum, preferably 2.9 × 108cfu/g；
Wherein, the formula of the primary inclined plane culture medium are as follows: KH2PO40.2g/L, MgSO40.2g/L, NaCl 0.2g,
CaCO35.0g, mannitol 10.0g, CaSO40.1g, pH 7.0, agar 20g, distilled water 1000mL；
The formula of secondary liquid culture medium are as follows: KH2PO40.2g/L, MgSO40.2g/L, NaCl 0.2g, CaCO3
5.0g, mannitol 10.0g, CaSO40.1g, pH 7.0, distilled water 1000mL；
The formula of three-level solid medium are as follows: mannitol 10.0g, yeast powder 1.0g, MgSO4·7H2O 0.2g, K2HPO4
0.5g, NaCl 0.1g, CaCl20.05g, Rh liquid microelement 4.0mL, H2O 1000mL, agar 20g, pH 6.8-7.0.
The present invention also provides the azotobacter MBC5 or growing nitrogen-fixing microbial inoculum the answering in soil improvement
With.
Optionally, the application includes that the microbial inoculum and sand 1:9-11 in mass ratio are applied to soil after mixing
Surface.
Optionally, the dosage of the microbial inoculum is 420-560g microbial inoculum/mu.
Invention provides a kind of growing nitrogen-fixing bacterium, and it is 8.1n that its nitrogenase activity is directly measured by acetylene reduction method
mol/μL·h.The bacterial strain is extracted from the desert ecosystem environment of Ningxia, China.This area's drought；It is annual and
Day and night temperature is big (Feng Wei, 2014)；Soil alkaline disaster is serious, and strongly, especially summer uitraviolet intensity is up to ultraviolet light
To 200-533 μ W/cm2(Pu Aiping, et al., 2005), radiation index is at 8 grades or so.And under normal circumstances in radiation intensity
In 70-130 μ W/cm2Ultraviolet light under, radiated time is differed from 15s to 30min, sterilizing rate up to 99% or more (Li Xiangyun,
Et al., 2006, Zhang Fengcai and Yuan Ersen, 1988, Zhang Fuyun and conditions are all, and 2005).The uitraviolet intensity of common sterilising conditions
With the uitraviolet intensity for being significantly lower than In Summer Over Ningxia when irradiation.The bacterial strain is in arid, the big temperature difference, saline and alkaline disaster and strong purple
Nitrogenase activity can be still lived and reproduced and had under UV radiation, and the especially bacterium is general thin to the highly resistant of ultraviolet light
Not available for bacterium, increases for ultraviolet radiation increasingly strong today in Global climate change Ozone hole, more have
Ecological significance and wide application value.Its manufactured microbial inoculum can be applied to the ecosystem of arid, high ultraviolet radiation,
And the nitrogen accumulation of these ecosystems can be increased, can play the role of improveing soil environment, improve microorganism and plant
The physiological activity of object increases net primary productivity.Experiment shows the microbial inoculum being seeded in Ningxia Margin of Southwest without the naked of nitrogenase activity
After 1 year, surface layer can detecte nitrogenase activity on husky surface, be 10.58n mol C2H2m-2h-1, and available nitrogen also increases
Add by 3.16mg kg-1Increase 8.9mg kg-1.Briefly, microbial inoculum made of the bacterial strain have production simple, low cost,
The advantages that drought-resistant, high UV light resistance and fixed nitrogen.
Preservation information: depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center China
General Microbiological Culture Collection Center (abbreviation CGMCC, address: Chaoyang District, Beijing City
The institute 3 of North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101)；Preservation date is on May 19th, 2015；
Collection number of registering on the books is CGMCC No.10821；The classification naming of the collection suggestion is Massilia
albidiflava；Strain number is MBC5.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
In the conventional means that are well known to those skilled in the art of technological means used, raw materials used is commercial goods.
Embodiment 1
The bacterial strain is extracted from the desert ecosystem surface layer of Ningxia, China.This area's drought；It is annual and round the clock
The temperature difference is big (Feng Wei, 2014)；Strongly, especially summer uitraviolet intensity is up to 200-533 μ W/cm for ultraviolet light2(Pu love
Duckweed, et al., 2005), radiation index is at 8 grades or so.And under normal circumstances in radiation intensity in 70-130 μ W/cm2It is ultraviolet
Under line, radiated time is differed from 15s to 30min, and sterilizing rate is up to 99% or more (Li Xiangyun, et al., 2006, Zhang Fengcai and
Yuan Ersen, 1988, Zhang Fuyun and conditions are all, and 2005).It is significantly lower than when the uitraviolet intensity of common sterilising conditions and irradiation peaceful
The uitraviolet intensity in summer in summer field, thus the bacterial strain with to ultraviolet light have highly resistant.
It weighs topsoil sample 10g and is put into the sterile water of the 100mL with bead and stand 20min, rotating
200r/min sufficiently vibrates 30min on formula shaking table, soil supension.Draw the above-mentioned soil of 5.0mL respectively with Sterile pipette
Suspension, which is added in 45mL sterile water, to be diluted, and is added to bacteria suspension on solid Ah 's shellfish culture medium with Sterile pipette, in 28 DEG C
Under the conditions of cultivate.Bacterial strain is purified with plate streak.The formula of Ah Xu shellfish culture medium used in the present invention are as follows: KH2PO4 0.2g/
L, MgSO40.2g/L, NaCl 0.2g, CaCO35.0g, mannitol 10.0g, CaSO40.1g, pH 7.0, agar 20g, distillation
Water 1000mL.
Bacterium will be screened and carry out Gram stain, then observe ne ar at microscope (OLYMPUS BX51)
And it takes pictures.
Then the 50 μ L of purifying bacterium colony in culture medium is inoculated on inclined-plane Ah 's shellfish solid medium with liquid-transfering gun, is placed on
It is cultivated three days for (30 DEG C) in incubator, then measures nitrogenase activity with acetylene reduction method.Preliminary result shows addition 20%
C2H2When, the nitrogenase activity highest that sample is showed, so C in this experiment2H2Amount accounts for the 20% of culture vessel volume.In order to
It compare experiment can with same experiments from now on, sample cultivated in illumination box to guarantee accurate environmental condition.For
The result is set to be more nearly true value, the environmental condition of condition of culture analog study area's summer.Incubation time is for 24 hours that preceding 16h has
Illumination, while temperature is 25 DEG C, rear 8 hours no lights, while temperature is 15 DEG C.50 μ L are extracted after culture from conical flask
Sample gas, note people, which has walked, measures ethylene emanation in the gas chromatograph (163 type gas chromatograph of Hitachi) of steady baseline.It is raw with ethylene
At amount (nmol C2H4/m-2h-1) nitrogenase activity of the azotobacter strain is described, for 8.1n mol/ μ Lh.
It will show then to carry out the bacterium with nitrogenase activity isolated with the method for 16S rDNA recognition sequence
Identification, process are as follows: using phenol and chloroform recovery DNA of bacteria, are dissolved with isopropanol, are saved in 4 DEG C.Then with 1.0 μ l
For (10pmol/ μ l) 27f and 1.0 μ l (10pmol/ μ l) 1496r as primer, 1.5 μ l DNA of bacteria are template, and 0.3 μ L is added
(2.5mmol/L each) dNTPs and 18.7 μ l ddH2O is made into 25 μ l reaction systems.This is subjected to PCR reaction, 30 are followed
Ring, denaturation, annealing and elongating temperature are 94 DEG C, 52 DEG C and 72 DEG C respectively, which completes on ABI-2720 instrument.PCR is produced
Object is detected with 1.5% agarose gel electrophoresis, and sample is saved in -20 DEG C.PCR product is sent to Huada gene company and is sequenced, it will
The mode bacterial sequences reported in column and ncbi database are sequenced and carry out similarity system design, it is recognized herein that sequence similarity is big
It is of the same race in 98% bacterial strain and mode bacterium.
Embodiment 2
Azotobacter MBC5 is inoculated in primary inclined plane culture medium, is cultivated 4 days at 30 DEG C, level-one bacterial strain is obtained；It will
Level-one strain inoculated is in secondary liquid culture medium, the shake culture 3 days under the conditions of 30 DEG C, obtains second level bacterial strain；By second level bacterium
Strain is inoculated in three-level solid medium, and acquisition three-level bacterial strain after 2 days is cultivated under the conditions of 30 DEG C；By three-level bacterial strain and turf
Contact obtains microbial inoculum after mixing；So that the content of azotobacter MBC5 is 2.9 × 10 in microbial inoculum8The pH value of cfu/g, microbial inoculum is
7.0。
Wherein, the formula of the primary inclined plane culture medium are as follows: KH2PO40.2g/L, MgSO40.2g/L, NaCl 0.2g,
CaCO35.0g, mannitol 10.0g, CaSO40.1g, pH 7.0, agar 20g, distilled water 1000mL；
The formula of secondary liquid culture medium are as follows: KH2PO40.2g/L, MgSO40.2g/L, NaCl 0.2g, CaCO3
5.0g, mannitol 10.0g, CaSO40.1g, pH 7.0, distilled water 1000mL；
The formula of three-level solid medium are as follows: mannitol 10.0g, yeast powder 1.0g, MgSO4·7H2O 0.2g, K2HPO4
0.5g, NaCl 0.1g, CaCl20.05g, Rh liquid microelement 4.0mL, H2O 1000mL, agar 20g, pH 6.8-7.0.
The Rh liquid microelement: H3BO35.0g, Na3MoO45.0g, H2O 1000mL。
Embodiment 3
Obtain mixture after the microbial inoculum prepared in embodiment 2 and sand are mixed according to mass ratio 1:10, then by the mixing
Object is sprinkled upon South of Maowusu Sandland upper soll layer, and it is about 500g microbial inoculum/mu that the dosage of microbial inoculum, which is dosage,.
After the microbial inoculum is seeded in South of Maowusu Sandland without the naked husky surface 1 year of nitrogenase activity, surface layer can be examined
Nitrogenase activity is measured, is 8.1n mol C2H2m-2h-1, and soil available nitrogen is also by 3.16mg kg-1Increase 8.9mg
kg-1。
Mu Us Shadi natural environmental condition is very severe, and major casualty weather has that arid, ultraviolet light is too strong, strong wind, sand
Cruelly, hot dry wind, frost, hail etc. are unfavorable for the existence and breeding of animals and plants and microorganism.Mu Us Shadi belongs to typical medium temperature
Band continental climate, the climate controlling factor is mainly northwest circulation, the northern continent air mass especially controlled for a long time, therefore
Day and night temperature is larger, and temperature on average is lower, and about 6-7 DEG C；This area's drought, average precipitation are about 280mm, year
Potential evaporation amount is 2100-2500mm, and frost-free period 128d, absolute frost-free period is 100d；Sandstorm is serious, and prevailing wind direction is west
North wind, annual mean wind speed are about 2.8m s-1, maximum wind velocity is up to 15-18m s-1, often have strong wind April November current year to next year
It, Windy Days are 45.8d, and at most up to 52d, annual sandstorm number of days is 20.6d；Soil alkaline degree is very high, and pH is
8.7-9.2；Strongly, especially summer uitraviolet intensity is up to 200-533 μ W/cm for ultraviolet light2, radiation index is on 8 grades of left sides
It is right.The discovery of the bacterial strain and the effect of inoculation of its microbial inoculum are illustrated for arid, the temperature difference is big, dust storm, saline and alkaline disaster and strong ultraviolet
Beta radiation has excellent tolerance, can effectively increase the nitrogen input capability of soil, increase soil available nitrogen and contain
Amount.In particular, being 70-130 μ W/cm in ultraviolet radiation intensity2When, radiated time is differed from 15s to 30min, and sterilizing rate is usual
Up to 99% or more.South of Maowusu Sandland summer is significantly lower than when the uitraviolet intensity of above-mentioned common sterilising conditions and irradiation
The uitraviolet intensity in field and time, it is special high to illustrate that general most bacteriums of the bacterial strain and microbial inoculum ratio have
UV light resistance.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (11)
1. a kind of azotobacter (Massilia albidiflava) MBC5, deposit number CGMCCNo.10821, preservation day
Phase is on May 19th, 2015, and depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center.
2. the microbial inoculum containing azotobacter MBC5 described in claim 1.
3. microbial inoculum according to claim 2, which is characterized in that also contain thallus adsorbent material, the bacterium in the microbial inoculum
Body adsorbent material is selected from least one of turf, perlite, vermiculite power and micro mist calcium carbonate.
4. microbial inoculum according to claim 3, which is characterized in that the thallus adsorbent material is turf.
5. microbial inoculum according to claim 4, which is characterized in that in the microbial inoculum, the content of azotobacter MBC5 is
2.1×108-2.9×108cfu/g。
6. microbial inoculum according to claim 5, which is characterized in that in the microbial inoculum, the content of azotobacter MBC5 is
2.9×108cfu/g。
7. the microbial inoculum according to any one of claim 2-6, which is characterized in that the pH value of the microbial inoculum is 6.8-7.2.
8. the preparation method of microbial inoculum described in any one of claim 2-7, which comprises the following steps:
The azotobacter MBC5 is inoculated in primary inclined plane culture medium, 28-30 DEG C culture 3-5 days, obtain level-one bacterium
Strain；By the level-one strain inoculated in secondary liquid culture medium, shake culture 2-3 days under the conditions of 28-30 DEG C obtains second level
Bacterial strain；By the second level strain inoculated in three-level solid medium, culture obtains three after 1-2 days under the conditions of 28-30 DEG C
Grade bacterial strain；It according to the content of azotobacter MBC5 in microbial inoculum is 2.1 × 10 by the three-level bacterial strain and thallus adsorbent material8-
2.9×108The proportion of cfu/g obtains microbial inoculum after mixing；
Wherein, the formula of the primary inclined plane culture medium are as follows: KH2PO40.2g/L, MgSO40.2g/L, NaCl 0.2g, CaCO3
5.0g, mannitol 10.0g, CaSO40.1g, pH7.0, agar 20g, distilled water 1000mL；
The formula of secondary liquid culture medium are as follows: KH2PO40.2g/L, MgSO40.2g/L, NaCl 0.2g, CaCO35.0g, sweet dew
Alcohol 10.0g, CaSO40.1g, pH7.0, distilled water 1000mL；
The formula of three-level solid medium are as follows: mannitol 10.0g, yeast powder 1.0g, MgSO4·7H2O 0.2g, K2HPO40.5g,
NaCl 0.1g, CaCl20.05g, Rh liquid microelement 4.0mL, agar 20g, H2O 1000mL, pH6.8-7.0.
9. microbial inoculum described in any one of azotobacter MBC5 described in claim 1 or claim 2-7 changes in soil
Application in good.
10. application according to claim 9, which is characterized in that including by the microbial inoculum and sand 1:9-11 in mass ratio
It is applied to soil surface after mixing.
11. application according to claim 9 or 10, which is characterized in that the dosage of the microbial inoculum be 420-560g microbial inoculum/
Mu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510859663.3A CN105255796B (en) | 2015-11-30 | 2015-11-30 | A kind of azotobacter MBC5 and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510859663.3A CN105255796B (en) | 2015-11-30 | 2015-11-30 | A kind of azotobacter MBC5 and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105255796A CN105255796A (en) | 2016-01-20 |
| CN105255796B true CN105255796B (en) | 2019-03-01 |
Family
ID=55095727
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510859663.3A Active CN105255796B (en) | 2015-11-30 | 2015-11-30 | A kind of azotobacter MBC5 and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105255796B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106833674B (en) * | 2017-02-09 | 2020-08-14 | 东莞市环境科学研究所 | Preparation method of heavy metal contaminated soil remediation agent |
-
2015
- 2015-11-30 CN CN201510859663.3A patent/CN105255796B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| Massilia dura sp. nov., Massilia albidiflava sp. nov.,Massilia plicata sp. nov. and Massilia lutea sp. nov.,isolated from soils in China;YuQingZhang,et al;《International Journal of Systematic and Evolutionary Microbiology》;20060228;全文 * |
| 小麦、水稻、玉米、白菜、芹菜内生固氮菌及其系统发育;孙建光等;《中国农业科学》;20120401(第7期);摘要、表1，第1307页 * |
| 毛乌素沙地土壤生物结皮细菌多样性分析与拮抗菌株5A5-3的筛选鉴定;刘慧娟;《中国优秀硕士学位论文全文数据库》;20110815(第8期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105255796A (en) | 2016-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102690767B (en) | Klebsiella oxytoca efficient in phosphorus solubilizing and nitrogen fixation and capable of inhibiting growth of pathogenic fungi | |
| CN105985917B (en) | Method for increasing biomass of chlorella in pig-raising wastewater | |
| CN103667132A (en) | Bacillus cereus and application thereof to improve plant drought-resistant capability | |
| WO2016155567A1 (en) | Streptomyces and method for producing milbemycin a4 using same | |
| CN105255797B (en) | A kind of azotobacter MBC7 and its application | |
| CN106399148B (en) | One kind secreting ammonium nitrogen-fixing bacteria and its application | |
| CN110846250B (en) | Bacillus subtilis capable of producing gamma-PGA in high yield and application thereof | |
| CN105349464B (en) | A kind of azotobacter MBC1 and its application | |
| CN105385624B (en) | A kind of azotobacter MBC8 and its application | |
| CN105255796B (en) | A kind of azotobacter MBC5 and its application | |
| CN105400716B (en) | A kind of azotobacter MBC9 and its application | |
| CN105400715B (en) | A kind of azotobacter MBC4 and its application | |
| CN105154353B (en) | A kind of bacillus subtilis and its application in greenhouse soil remediation | |
| CN114958683B (en) | Bacillus and application thereof | |
| CN105385626B (en) | A kind of azotobacter MBC10 and its application | |
| CN105385627B (en) | A kind of azotobacter MBC3 and its application | |
| CN103343097B (en) | Nitrogen fixing micrococcus and application thereof | |
| CN105385625B (en) | A kind of azotobacter MBC2 and its application | |
| CN103695335B (en) | Mesorhizobium huakuii and use thereof | |
| CN105462885A (en) | Phenolic acid degrading bacterium, biological organic fertilizer and application of phenolic acid degrading bacterium | |
| CN114164140B (en) | Efficient phosphorus-dissolving bacteria MQR6 and fermentation product and application thereof | |
| CN102417891B (en) | Bacteria with crude oil degrading function and biological repair agent suitable for crude oil polluted soil in low-temperature and saline-alkali districts | |
| CN109609412A (en) | A kind of Thermophilic Bacteria Bacillus smithii Ths1 and its application | |
| CN103333837B (en) | One strain fixed nitrogen Arthrobacter and uses thereof | |
| CN107513508A (en) | A kind of enterobacteria and its application in terms of purifying water body |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |