CN105255755A - Microbial strain for easing tebuconazole seed coating agent phytotoxicity, bacterial preparation substance and application thereof - Google Patents

Microbial strain for easing tebuconazole seed coating agent phytotoxicity, bacterial preparation substance and application thereof Download PDF

Info

Publication number
CN105255755A
CN105255755A CN201510627934.2A CN201510627934A CN105255755A CN 105255755 A CN105255755 A CN 105255755A CN 201510627934 A CN201510627934 A CN 201510627934A CN 105255755 A CN105255755 A CN 105255755A
Authority
CN
China
Prior art keywords
bacterial
preparation
tebuconazole
bacterial preparation
coat agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510627934.2A
Other languages
Chinese (zh)
Inventor
李秀颖
李旭
李丹
孙慧
魏婷婷
王宇
王芳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenyang Research Institute of Chemical Industry Co Ltd
Original Assignee
Shenyang Research Institute of Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenyang Research Institute of Chemical Industry Co Ltd filed Critical Shenyang Research Institute of Chemical Industry Co Ltd
Priority to CN201510627934.2A priority Critical patent/CN105255755A/en
Publication of CN105255755A publication Critical patent/CN105255755A/en
Pending legal-status Critical Current

Links

Abstract

The invention relates to the field of microbial technologies, in particular to a microbial strain for easing tebuconazole seed coating agent phytotoxicity, a bacterial preparation substance and application thereof. The microbial strain provided by the invention is Bacillus mucilaginosus. The bacterial preparation substance provided by the invention can enhance crop seed vigor, also can eliminate or alleviate the chemical pesticide phytotoxicity of the tebuconazole seed coating agent on crop seeds to a certain extent, and has certain application potential in the seed coating agent industry.

Description

A kind of microorganism strains for alleviating the poisoning of tebuconazole seed coat agent, bacterial preparation and application
Technical field
The present invention relates to microbial technology field, being specifically related to a kind of microorganism strains for alleviating the poisoning of tebuconazole seed coat agent, bacterial preparation and application.
Background technology
Corn is the important farm crop of China, in national economic development process, have critical role.Seed coat agent plays an important role in control of maize disease and pest, and can promote plant growth again, and volume increase, has significant economy and ecological benefits.The main component of current corn seed coating agent is sterilant (such as: Provado, Chlorpyrifos 94, Volaton etc.) and sterilant (such as: thiram, triazole species, fludioxonil etc.).Above-mentioned sterilant and sterilant can play the effect of good prevention and elimination of disease and pests, but as chemical pesticide, it can not be ignored the poisoning of seed, as triazole bactericidal agent can suppress germination and the growth of corn in various degree,, after spring ploughing, if there is cold spell in later spring in especially tebuconazole seed coat agent Maize Seeds Coated, the poisoning incidence of tebuconazole is very high, causes have a strong impact on spring ploughing.Therefore, the side effect of seed coat agent seriously governs the healthy and stable development of corn seed coating agent industry, and existing seed coat agent applying aborning.
Given this, overcome above defect of the prior art, provide a kind of tebuconazole seed coat agent of alleviating to become this area technical problem urgently to be resolved hurrily to the preparation of the poisoning of the farm crop such as corn or method.
Summary of the invention
The object of the invention is to the above-mentioned defect for prior art, a kind of microorganism strains for alleviating the poisoning of tebuconazole seed coat agent is provided.
Object of the present invention realizes by following technical measures:
A kind of microorganism strains for alleviating the poisoning of tebuconazole seed coat agent, described bacterial strain is silicate bacteria (Bacillusmucilaginosus) SY-FX-77 bacterial strain, the preserving number of described bacterial strain is CCTCCM2015434, depositary institution: China typical culture collection center, preservation date: on July 6th, 2015.
Further, the 16SrRNA gene order of described bacterial strain is SEQIDNo.1.
Present invention also offers a kind of bacterial preparation, the pure growth of described bacterial preparation to be preserving number the be bacterial strain of CCTCCM2015434 or culture enriched material or bacterial suspension or fermented liquid.
Preferably, the bacterial strain of described bacterial preparation to be preserving number be CCTCCM2015434 cultivates the fermented liquid of formation by fermentation in fermentation culture.
Preferably, consisting of of described fermentation culture: sucrose 5g, Na 2hPO 42g, MgSO 47H 2o0.5g, FeCl 30.005g, CaCO 30.1g, bauxite 0.5g, water 1000ml.
Preferably, consisting of of described fermentation culture: Tryptones 10g, yeast extract 5g, sodium-chlor 10g, water 1000mL.
Preferably, described bacterial preparation is through cryodesiccated prepared product.
Present invention also offers the application of above-mentioned bacterial strain in the preparation of preparation alleviation tebuconazole seed coat agent poisoning.
Present invention also offers the application of above-mentioned bacterial preparation in the preparation of preparation alleviation tebuconazole seed coat agent poisoning.
Microorganism strains of the present invention is silicate bacteria (Bacillusmucilaginosus), bacterial preparation of the present invention can improve crop seeds vigor, eliminate to a certain extent or alleviate the chemical pesticide poisoning of tebuconazole seed coat agent to crop seeds, in seed coat agent industry, there is certain application potential.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with specific embodiment, the present invention is described in further detail.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Term " fermentation culture " refers to liquid nutrient medium; Term " fermented liquid " refers to the liquid (including nutrient solution) formed after fermentation culture for some time in bacterial strain liquid medium within; Term " bacterial suspension " is fermented liquid collecting precipitation (strain culture or thalline) after centrifugal treating, to precipitate in water-soluble or damping fluid, and through concussion or piping and druming, the strain culture precipitating shape be suspended formed homogeneous suspension liquid.
Present embodiments provide a kind of microorganism strains for alleviating the poisoning of tebuconazole seed coat agent, described bacterial strain is silicate bacteria (Bacillusmucilaginosus) SY-FX-77 bacterial strain, the preserving number of described bacterial strain is CCTCCM2015434, depositary institution: China typical culture collection center, preservation date: on July 6th, 2015.
Further, described identification of strains feature is as follows: (1), in the growth of silicate bacteria substratum, half glass is spherical, protruding, water white transparency, and after 5-6 days, there is cloud point at middle part, and edge is transparent, smooth surface, thickness, and elasticity is large, can pull into thread.(2) in the growth of LB substratum, thalline is creamy white, circular opaque colony, bacterium colony surface and the smooth of the edge, without protruding.
Further, the 16SrRNA gene order of described bacterial strain is SEQIDNo.1.Carry out tetraploid rice according to 16SrRNA gene order in sequencing result and Genbank, result shows that SY-FX-77 bacterial strain belongs to silicate bacteria (Bacillusmucilaginosus).
Bacterial preparation provided by the invention is the pure growth of above-mentioned bacterial strains or culture enriched material or fermented liquid, can be that above-mentioned bacterial strains forms pure growth at solid culture primary surface, such as: the bacterium colony that Solid media for plates or inclined-plane solid medium are formed, also can be the bacterial suspension through centrifugation gained after fermentation culture in above-mentioned bacterial strains liquid medium within (fermentation culture), can also be the fermented liquid that in above-mentioned bacterial strains liquid medium within (fermentation culture), growth fermentation is formed.
Particularly, the cultural method of silicate bacteria SY-FX-77 provided by the present invention comprises: by described silicate bacteria in storage medium, at 30-37 DEG C of activation 12-24h; After activation, bacterial strain is in seed culture medium, cultivates 1-2 days, as seed liquor in 30-37 DEG C, 100-150r/min; Gained seed liquor is inoculated in fermention medium with 4-8% (v/v) inoculative proportion, at 30-37 DEG C, 100-150r/min shaking culture 12-36h.
Described isolation medium, storage medium and seed culture medium are same (silicate bacteria substratum): sucrose 5g, Na 2hPO 42g, MgSO 47H 2o0.5g, FeCl 30.005g, CaCO 30.1g, bauxite 0.5g, water 1000ml, pH7.0-7.5.
In embodiments of the invention, preferably using LB substratum as fermention medium, also can using storage medium or other substratum that can be applicable to this strain growth as fermention medium, described fermention medium (LB substratum): Tryptones 10g/L, yeast extract 5g/L, sodium-chlor 10g/L, water 1000mL, pH7.0-7.5.
Cultivate silicate bacteria (Bacillusmucilaginosus) SY-FX-77 with above-mentioned fermention medium and obtain fermented liquid, this fermented liquid effectively can alleviate the low temperature drug injury problem that tebuconazole seed coat agent dressing corn brings, effectively alleviate that the corn seed germination rate that poisoning causes is low, root is long and the downtrod situation of the long-living length of bud, Maize Seeds Coated percentage of germination can be improved, promote that root extends.Bibliographical information is yet there are no about the application of silicate bacteria in the poisoning of seed coat agent solution.
Embodiment 1
the separation of bacterial strain SY-FX-77
Get oil-polluted soils 10g, add in the triangular flask of in-built 100mL sterilized water and granulated glass sphere, vibrate 30min on 120r/min shaking table, and vibration terminates rear standing 1h, and get supernatant 0.5mL and add in 4.5mL sterilized water, vortex mixed, dilutes 10 successively -2, 10 -3, 10 -4doubly, get 150 μ L diluents respectively and coat on silicate bacteria culture medium flat plate, each gradient be coated with 3 parallel, cultivate 12-48h at 35 DEG C, the single bacterium colony on picking substratum carries out plate streaking purifying, obtains silicate bacteria bacterial strain.
the qualification of bacterial strain SY-FX-77
Carry out identification of morphology and Molecular Identification respectively to obtained strains SY-FX-77, result is as follows:
Identification of morphology result: (1), in the growth of silicate bacteria substratum, half glass is spherical, protruding, water white transparency, and after 5-6 days, there is cloud point at middle part, and edge is transparent, smooth surface, thickness, and elasticity is large, can pull into thread.(2) in the growth of LB substratum, thalline is creamy white, circular opaque colony, bacterium colony surface and the smooth of the edge, without protruding;
Molecular Identification result: the 16SrRNA gene order of bacterial strain is SEQIDNo.1.Carry out tetraploid rice according to 16SrRNA gene order in sequencing result and Genbank, result shows that SY-FX-77 bacterial strain belongs to silicate bacteria (Bacillusmucilaginosus).
According to above qualification result, SY-FX-77 bacterial strain belongs to silicate bacteria, is carried out preservation, and preservation information is as follows: depositary institution is China typical culture collection center, and preservation date is on July 6th, 2015, and preserving number is CCTCCM2015434.
Embodiment 2
Embodiments of the invention 2 provide a kind of bacterial preparation, and this bacterial preparation is the pure growth of the SY-FX-77 bacterial strain of embodiment 1 gained or culture enriched material or bacterial suspension or fermented liquid.
Wherein, bacterial preparation can also be through cryodesiccated prepared product.
The cultivation of embodiment 1 gained SY-FX-77 bacterial strain
the preparation of substratum
Preservation isolation medium and seed culture medium (silicate bacteria substratum): sucrose 5g, Na 2hPO 42g, MgSO 47H 2o0.5g, FeCl 30.005g, CaCO 30.1g, bauxite 0.5g, water 1000ml, pH7.0-7.5.
Fermention medium (LB substratum): Tryptones 10g/L, yeast extract 5g/L, sodium-chlor 10g/L, water 1000mL, pH7.0-7.5.
Above-mentioned substratum 121 DEG C of high pressure steam sterilization 20min, stand-by.
actication of culture
By silicate bacteria (Bacillusmucilaginosus) SY-FX-77 switching seed slant medium, in constant incubator, cultivate 24h for 35 DEG C.
seed culture
The good activated inclined plane strain inoculation of growth selection in being equipped with in above-mentioned obtained seed culture medium triangular flask (the in-built 100mL substratum of 250mL triangular flask), in 35 DEG C, 120r/min shaking culture 24h.
fermentation culture
The fermention medium configured is placed in fermentor tank, sterilizing 20min under 121 DEG C of conditions, to be cooled to accessing 4% preactivated good kind daughter bacteria liquid during room temperature after sterilizing completes.Now controlling fermentor tank rotating speed is 120r/min, and air flow is 1:1-1:1.2, and temperature is 35 DEG C, and cultivate 24h, in culturing process, automatic powder adding adds organic silicon oil foam killer.
Embodiment 3
the solution poisoning experiment of the preparation containing embodiment 1 gained SY-FX-77 bacterial strain prepared product
The present embodiment is described for the fermented liquid of SY-FX-77
Seed coat agent: 6% tebuconazole seed coat agent.
Addition manner: the fermented liquid of silicate bacteria (Bacillusmucilaginosus) SY-FX-77 is directly added in seed coat agent by different ratios, mixes, dressing is carried out to seed.Do not contain bacterium dressing for directly seed coat agent is carried out dressing to seed.Blank is the grouping not adding seed coat agent.
Corn seed: Zheng Dan 985.
Pesticide-seeds ratio: 1:500.
Germination condition: before corn germination, whole process is placed in 10 DEG C of environment, and the low temperature of cold spell in later spring during simulation spring ploughing, makes tebuconazole dressing produce poisoning.
Table 1 experimental result
Upper table result shows, the fermented liquid of bacterial strain SY-FX-77 adds in tebuconazole seed coat agent by different ratios, and dressing is carried out to corn seed, can alleviate tebuconazole preferably at low temperatures to the poisoning that corn germinal produces, under tebuconazole poisoning effect, corn germination rate is all less than 80%, add the seed coat agent dressing corn of above-mentioned substance, corn germination rate can be made to bring up to more than 90%, suitable with the process of blank corn seed, and can effectively promote that Zea mays root is extended.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.

Claims (9)

1. for alleviating a microorganism strains for tebuconazole seed coat agent poisoning, it is characterized in that, the preserving number of described bacterial strain is CCTCCM2015434.
2. the microorganism strains for alleviating the poisoning of tebuconazole seed coat agent according to claim 1, is characterized in that, the 16SrRNA gene order of described bacterial strain is SEQIDNo.1.
3. a bacterial preparation, is characterized in that, the pure growth of described bacterial preparation to be preserving number the be bacterial strain of CCTCCM2015434 or culture enriched material or bacterial suspension or fermented liquid.
4. bacterial preparation according to claim 3, is characterized in that, the bacterial strain of described bacterial preparation to be preserving number be CCTCCM2015434 cultivates the fermented liquid of formation by fermentation in fermentation culture.
5. bacterial preparation according to claim 4, is characterized in that, consisting of of described fermentation culture: sucrose 5g, Na 2hPO 42g, MgSO 47H 2o0.5g, FeCl 30.005g, CaCO 30.1g, bauxite 0.5g, water 1000ml.
6. bacterial preparation according to claim 4, is characterized in that, consisting of of described fermentation culture: Tryptones 10g, yeast extract 5g, sodium-chlor 10g, water 1000mL.
7. bacterial preparation according to claim 3, is characterized in that, described bacterial preparation is through cryodesiccated prepared product.
8. the bacterial strain described in claim 1 or 2 alleviates the application in the preparation of tebuconazole seed coat agent poisoning in preparation.
9. the bacterial preparation described in any one of claim 3 to 7 alleviates the application in the preparation of tebuconazole seed coat agent poisoning in preparation.
CN201510627934.2A 2015-09-28 2015-09-28 Microbial strain for easing tebuconazole seed coating agent phytotoxicity, bacterial preparation substance and application thereof Pending CN105255755A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510627934.2A CN105255755A (en) 2015-09-28 2015-09-28 Microbial strain for easing tebuconazole seed coating agent phytotoxicity, bacterial preparation substance and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510627934.2A CN105255755A (en) 2015-09-28 2015-09-28 Microbial strain for easing tebuconazole seed coating agent phytotoxicity, bacterial preparation substance and application thereof

Publications (1)

Publication Number Publication Date
CN105255755A true CN105255755A (en) 2016-01-20

Family

ID=55095687

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510627934.2A Pending CN105255755A (en) 2015-09-28 2015-09-28 Microbial strain for easing tebuconazole seed coating agent phytotoxicity, bacterial preparation substance and application thereof

Country Status (1)

Country Link
CN (1) CN105255755A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106479926A (en) * 2016-10-25 2017-03-08 吉林农业大学 Tebuconazole pesticide degradation bacteria and the geobiont repair microbial inoculum based on the bacterium and its application
CN108218555A (en) * 2017-12-12 2018-06-29 铜陵硕丰生态科技有限公司 A kind of mating dressing agent for rice alleviant
CN108249982A (en) * 2017-12-12 2018-07-06 铜陵硕丰生态科技有限公司 A kind of mating peanut seed coat agent alleviant

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103718812A (en) * 2013-12-26 2014-04-16 董金锋 Wheat high-yield cultivation method
WO2014145883A1 (en) * 2013-03-15 2014-09-18 Spogen Biotech Inc. Plant growth-promoting bacteria and methods of use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014145883A1 (en) * 2013-03-15 2014-09-18 Spogen Biotech Inc. Plant growth-promoting bacteria and methods of use
CN103718812A (en) * 2013-12-26 2014-04-16 董金锋 Wheat high-yield cultivation method

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
INOUE，K.ET AL: "ACCESSION NO. AB196250.1", 《GENBANK》 *
SHELOBOLINA，E.S.ET AL.: "ACCESSION NO. NR_028675.1", 《GENBANK》 *
XIU-FANG HU,ET AL.: "Transfer of Bacillus mucilaginosus and Bacillus edaphicus to the genus Paenibacillus as Paenibacillus mucilaginosus comb.nov.and Paenibacillus edaphicus comb.nov.", 《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》 *
常文智: "胶质类芽孢杆菌溶磷分子机理探索及其对土壤生态效应的影响", 《中国优秀硕士学位论文全文数据库农业科技辑》 *
杨会营等: "含有不同生长调节剂3%戊唑醇悬浮种衣剂对玉米种子萌发和生长效应的影响", 《安徽农业科学》 *
王振亚: "胶质类芽孢杆菌3016基因组学初步研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 *
王璇: "胶质类芽孢杆菌3016全基因组测序及菌种水平特异分子标识的筛选和鉴定", 《中国优秀硕士学位论文全文数据库基础科学辑》 *
蒋先军等: "硅酸盐细菌代谢产物对植物生长的促进作用", 《西南农业大学学报》 *
高仁君等: "戊唑醇和三唑醇种衣剂对小麦幼苗生长发育的影响", 《植物保护学报》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106479926A (en) * 2016-10-25 2017-03-08 吉林农业大学 Tebuconazole pesticide degradation bacteria and the geobiont repair microbial inoculum based on the bacterium and its application
CN106479926B (en) * 2016-10-25 2019-07-12 吉林农业大学 Tebuconazole pesticide degradation bacteria and geobiont repair microbial inoculum and its application based on the bacterium
CN108218555A (en) * 2017-12-12 2018-06-29 铜陵硕丰生态科技有限公司 A kind of mating dressing agent for rice alleviant
CN108249982A (en) * 2017-12-12 2018-07-06 铜陵硕丰生态科技有限公司 A kind of mating peanut seed coat agent alleviant

Similar Documents

Publication Publication Date Title
CN102839142B (en) Bacillus amyloliquefaciens (Ba 168) and fermenting culture method and application of bacillus amyloliquefaciens
CN109182215A (en) One bacillus licheniformis bacterial strain and its application in inhibition bacterial pathogen
CN102676435A (en) Paenibacillus polymyxa for antagonizing Fusarium oxysporum in rhizosphere soil of Radix Pseudostellariae
CN106719844B (en) It is a kind of for preventing and treating the biological seed coating agent and preparation method thereof of cucurbita pepo root rot
CN104877926B (en) The bacterial strain of one plant of antagonism smoke tree wilt and its application
CN106754484A (en) One plant of rhizobium leguminosarum and its fermentation culture method and application
CN113755393B (en) Bacillus beilesensis HP-24 and application thereof in preparation of bacterial liquid for preventing and treating bacterial fruit blotch of melons
CN106942276A (en) Bacillus subtilis BAB 1 prevents and treats the application of cucurbits powdery mildew
CN108220194B (en) Streptomyces parvus with disease prevention and growth promotion functions and application thereof
CN105255755A (en) Microbial strain for easing tebuconazole seed coating agent phytotoxicity, bacterial preparation substance and application thereof
CN104232532A (en) Endophytic bacillus pumilus, and microbial agent, preparation method and application of endophytic bacillus pumilus
CN105420172B (en) Microbial inoculum and the application of a kind of metribuzin pesticide residual degrading bacteria and its production
CN108865934B (en) Bacillus safensis HMD9204 and microbial inoculum and application thereof
CN106035378A (en) Compound bacterium agent for preventing and treating bacterial diseases of tobacco
CN100371436C (en) Bacterium for degrading chlorpyrifos pesticide residue and produced bacterium formulation
CN100368528C (en) Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation
CN105494443A (en) Compound microorganism agent and application thereof
CN102154155B (en) Brevibacillus brevis for preventing and treating plant fungus diseases and method for preparing biopesticide
CN103289931B (en) Bacillus vallismortis strain SJ and application thereof in preparation of tobacco antiviral preparation and promoter
CN116904354A (en) Priestia aryabhattai H2 and application thereof
CN106811435A (en) A kind of Alpine Grasslands herbage endogenetic bacteria Bacillus amyloliquefaciens strain, microbial bacterial agent and its preparation method and application
CN111109026A (en) Biological control method for diseases in rice growth process and application
CN103525710B (en) Penicillium oxalicum BAM-1 and separation purification method thereof and application
CN103451116A (en) Pseudomonas fluorescens Y13, and preparation method and applications thereof
CN109207393A (en) One bacillus amyloliquefaciens and its application in prevention and treatment celery root rot

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160120