CN105250246A - Application of protocatechualdehyde in treatment of male infertility and in-vitro treatment of sperms - Google Patents
Application of protocatechualdehyde in treatment of male infertility and in-vitro treatment of sperms Download PDFInfo
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Abstract
The invention relates to application of protocatechualdehyde in treatment of male infertility and in-vitro treatment of sperms, belonging to the technical field of treatment of male infertility and in-vitro treatment of sperms. According to a technical scheme in the invention, protocatechualdehyde is applied to treatment of male infertility and in-vitro treatment of sperms; protocatechualdehyde is cheap and can be popularized and applied for treatment of male infertility; protocatechualdehyde has oxidative stress resistance, can increase the content of DJ-1 protein in a testis and is superior to carnitine and vitamin E in treatment of the mechanism of male infertility; when protocatechualdehyde is used for in-vitro treatment of sperms, oxidative stress resistance of protocatechualdehyde can be given to play, oxyradicals generated in in-vitro treatment of sperms can be eliminated, so the morphology and functions of the sperms are protected; and utilization of protocatechualdehyde exerts better effect compared with usage of only a sperm nutrient solution, and protocatechualdehyde is cheap in price and convenient to use (wherein protocatechualdehyde may be directly added into a nutrient solution) and does not lead to obvious increase in operation steps, time and operation cost.
Description
Technical field
The present invention relates to the application of a kind of protocatechualdehyde in treatment male infertility and extracorporeal treatment sperm, particularly the application in the male infertility caused by cyclophosphamide treated by protocatechualdehyde, and protocatechualdehyde is for the application of Male reproductive damage preventing and treating cyclophosphamide and cause, and belongs to treatment male infertility and extracorporeal treatment sperm technical field.
Background technology
Along with the aggravation of industrialized development, environmental pollution and the increase of social pressure, male semen quality declines year by year, and male infertility patient obviously increase.By World Health Organization (WHO) (WorldHealthOrganization, WHO) standard, the couple at child-bearing age lives together more than 1 year after marriage, does not adopt any contraceptives, causes the infertile person in the wife's side, be called male infertility by bridegroom's or husband's side reason.The treatment of male infertility mainly comprises Issues of Human Assisted Reproductive Technologies, Drug therapy improves sperm quality, and operative treatment corrects physiological structure extremely.Cause the cause of disease of male infertility very complicated, not yet find the definite cause of disease causing male infertility at present, so to male infertility mainly empirical Drug therapy.Clinical conventional medical treatment regime has: Antioxidation Treatment, comprises conventional vitamin E, coenzyme Q10 etc.; Hormone therapy, comprises estrogen antagonist, promoting sexual gland hormone, gonadotropin releasing hormone, androgen, Aromatase inhibitor; Other drug, comprises carnitine etc.But these medicines have respective limitation, as expensive, side reaction is many, therefore research and develop new male infertility medicine there is important clinical meaning and application prospect widely.
Issues of Human Assisted Reproductive Technologies can be used for treatment male infertility and infertility, and comprise artificial insemination and IVF-ET Technique, two kinds of technology all need to carry out the preferred sperm of extracorporeal treatment to semen.The preferred sperm of sperm culture is the required step of routine in Issues of Human Assisted Reproductive Technologies treatment, and object obtains the sperm suspension not having refining, cell debris and microbiological contamination, reclaims the sperm that sufficient amount has normal morphology and function.Need in liquefacient duration process to add sperm nutrient solution to maintain motility of sperm and function.At present, background technology sperm nutrient solution used still haves much room for improvement, and what China was used is mostly imported product, expensive.The sperm nutrient solution composition that exploitation can improve motility of sperm preferably has pivotal role and significance with raising supplementary reproduction Clinical Pregnancy Rate in for sperm in vitro.
Protocatechualdehyde (protocatechuicaldehyde, PAL) formal name used at school 3,4-4-dihydroxy benzaldehyde, it is one of primary product of red sage root water soluble ingredient salvianolic acid B degraded, be present in the plants such as labiate Radix Salviae Miltiorrhizae, also be present in the leaf of Lindsaeaceae plant Folium Stenolomatis, in holly plant Folium Ilicis Purpureae, originate very extensive.Research shows, protocatechualdehyde has pharmacologically active widely, comprises the aspect such as atherosclerosis, protection cardiac muscle, antithrombus formation, neuroprotective, anti-pus and blood, antiviral, fibrosis.Up to now, about the purposes of protocatechualdehyde in treatment male infertility and extracorporeal treatment sperm does not also have bibliographical information.
Summary of the invention
The object of the invention is to provide the application of a kind of protocatechualdehyde in treatment male infertility and extracorporeal treatment sperm, solves the problems referred to above existed in background technology.
Technical scheme of the present invention is:
The application of protocatechualdehyde in treatment male infertility and extracorporeal treatment sperm, is used for the treatment of male infertility and extracorporeal treatment sperm by protocatechualdehyde.
Described protocatechualdehyde is used for the treatment of male infertility, and protocatechualdehyde aqueous stability is poor, uses solid preparation, as tablet, granule, capsule, soft capsule, drop pill etc.
Dosage: convert according to zoopery, recommended dose is that 4.4mg/kg(is about 300mg/d).
Administrated method: oral.
Drug treatment: recommend 1-2 spermatogenic cycle, namely 3-6 month.
Can be used alone, also can share with other drug.
Described protocatechualdehyde is used for extracorporeal treatment sperm, and protocatechualdehyde aqueous stability is poor, makes injectable powder, matching while using, after a small amount of physiological saline solution, adds in sperm nutrient solution and uses, and recommended density scope is 0.1mg/ml-1mg/ml.
The present invention is proved by test; protocatechualdehyde can increase testis index and the epididymis index of the chmice in male sterile that cyclophosphamide causes, and improves Maturation of sperm nucleus, and protection testis tissue form is normal; increase SOD in testis active, improve DJ-1 protein content in testis.Protocatechualdehyde has the pharmacologically active for the treatment of male infertility.
The present invention is proved by experiment in vitro, after protocatechualdehyde and sperm are hatched in vitro, can significantly improve living spermatozoa percentage and sperm membrane integrity, and processing sperm aspect in vitro has certain application prospect.
The treatment of male infertility mainly comprises Drug therapy and Issues of Human Assisted Reproductive Technologies.The medicine limitednumber of male infertility, needs the medicine that exploitation is new badly.Must carry out extracorporeal treatment to sperm in Issues of Human Assisted Reproductive Technologies, the sperm nutrient solution needed for it is still to be improved.The present invention finds, protocatechualdehyde can be used for treatment and the dealing sperm of male infertility, and has beat all technique effect.
In the present invention, the derivant cyclophosphamide of male infertility is the antitumor drug and immunosuppressant commonly used clinically, effective and low price, but cyclophosphamide has obvious male genetic toxicity, limits its application to a great extent.The present invention shows, protocatechualdehyde also may be used for the Male reproductive damage that control cyclophosphamide causes, thus promotes cyclophosphamide use clinically, also can be patient and reduces the financial burden.
The advantage of protocatechualdehyde treatment male infertility and beneficial effect:
For male infertility, current clinical conventional medical treatment regime has: hormone therapy, comprises estrogen antagonist, promoting sexual gland hormone, gonadotropin releasing hormone, androgen, Aromatase inhibitor; Antioxidation Treatment, comprises conventional vitamin E, coenzyme Q10 etc.; Other drug, comprises carnitine etc.
(1) hormone therapy: estrogen antagonist curative effect is not affirmed, is not suitable for long-term treatment; The offer limited effectiveness of promoting sexual gland hormone, applies less; Costly, curative effect is not good enough for gonadotropin releasing hormone, generally not recommendation; Androgen in treating is to pregnancy rate and be not improved; Aromatase inhibitor can not improve semen quality, and expensive, therefore clinical less use.As can be seen here, hormone therapy is clinically and be of little use, and expensive; And protocatechualdehyde abundance, therefore relative low price, can promote the use of.
(2) Antioxidation Treatment: vitamin E curative effect is clearer and more definite, its mechanism of action mainly anti-oxidation stress, vitamin E is water insoluble, containing edible oil in preparation, treatment male infertility long-term taking Vitamin E preparation may cause patient's fat intake to increase, thus initiation relevant disease, as dyslipidemia etc.; And protocatechualdehyde good water solubility, patient's fat intake can not be increased.Coenzyme Q10 (400mg/d, 6 months) can improve patient's semen quality, and by drug price, patient's daily cost about 12 yuan, improves semen quality (6 months) expense about 2160 yuan; And protocatechualdehyde price is cheaper.The mechanism of action of vitamin E and coenzyme Q10 is antioxidation, and protocatechualdehyde is except to except anti-oxidation stress, also having other mechanism of action (increasing testis DJ-1 protein content).
(3) other: carnitine is applied more extensive at present, carnitine treatment male infertility common dose is 2-3g/d, with reference to drug price, patient daily cost 22-33 unit, 1 course for the treatment of (3 months) medical expense 1980-2970 unit, 2 course for the treatment of (6 months) medical expense 3960-5940 units, expense is relatively high; And protocatechualdehyde abundance, and every consumption per day few (300mg/d), therefore medical expense is lower than carnitine.The mechanism of action mainly antioxidation of carnitine, and protocatechualdehyde is except to except anti-oxidation stress, also having other mechanism of action (increasing testis DJ-1 protein content).In sum, it is carnitine and vitamin E that current clinical treatment male infertility applies more medicine, the main mechanism of the two is anti-oxidation stress, and protocatechualdehyde is except having anti-oxidative stress, testis DJ-1 protein content can also be improved, treatment male infertility mechanism is better than carnitine and vitamin E.Therefore, we think, protocatechualdehyde can be used alone treatment male infertility clinically, also can with carnitine and vitamin E conbined usage.
The advantage of protocatechualdehyde extracorporeal treatment sperm and beneficial effect:
Sperm nutrient solution conventional at present has Ham ' s-F10, Earle ' s, HTF etc., its main component is all various inorganic salt and aminoacid, does not add other compositions.In sperm nutrient solution, add protocatechualdehyde, its anti-oxidative stress can be played, remove the oxygen-derived free radicals produced in dealing sperm process, thus protection sperm morphology and function, than applying sperm nutrient solution effect more separately.In addition, protocatechualdehyde is cheap, can not increase the running cost of extracorporeal treatment sperm, and (directly adding in nutritional solution) easy to use, can not obviously increase operating procedure and time.
Accompanying drawing explanation
Fig. 1 is protocatechualdehyde of the present invention causes the Testis Morphology of chmice in male sterile impact (× 400) on cyclophosphamide;
Fig. 2 be protocatechualdehyde of the present invention cyclophosphamide is caused the testis tissue SOD activity of chmice in male sterile impact (
± S, n=10);
Fig. 3 be protocatechualdehyde of the present invention cyclophosphamide is caused the testis DJ-1 protein expression of chmice in male sterile impact (
± S, n=3).
Detailed description of the invention
Below in conjunction with accompanying drawing, by example, the invention will be further described.
The application of protocatechualdehyde in treatment male infertility and extracorporeal treatment sperm, is used for the treatment of male infertility and extracorporeal treatment sperm by protocatechualdehyde.
Described protocatechualdehyde is used for the treatment of male infertility, uses solid preparation;
Dosage: 4.4mg/kg(is about 300mg/d);
Administrated method: oral.
Drug treatment: a 1-2 spermatogenic cycle, namely 3-6 month.
Can be used alone, also can share with other medicines.
Described protocatechualdehyde is used for extracorporeal treatment sperm, and make injectable powder, matching while using, after a small amount of physiological saline solution, add in sperm nutrient solution and use, concentration is 0.1mg/ml-1mg/ml.
The present invention is proved by test; protocatechualdehyde can increase testis index and the epididymis index of the chmice in male sterile that cyclophosphamide causes, and improves Maturation of sperm nucleus, and protection testis tissue form is normal; increase SOD in testis active, improve DJ-1 protein content in testis.Protocatechualdehyde has the pharmacologically active for the treatment of male infertility.
The present invention is proved by experiment in vitro, after protocatechualdehyde and sperm are hatched in vitro, can significantly improve living spermatozoa percentage and sperm membrane integrity, and processing sperm aspect in vitro has certain application prospect.
The treatment of male infertility mainly comprises Drug therapy and Issues of Human Assisted Reproductive Technologies.The medicine limitednumber of male infertility, needs the medicine that exploitation is new badly.Must carry out extracorporeal treatment to sperm in Issues of Human Assisted Reproductive Technologies, the sperm nutrient solution needed for it is still to be improved.The present invention finds, protocatechualdehyde can be used for treatment and the dealing sperm of male infertility, and has beat all technique effect.
Concrete test is as follows:
1 experiment material
1.1 key agents and reagent
Protocatechualdehyde, Beijing lark prestige Science and Technology Ltd., batch number: LC70N14; Cyclophosphamide for injection, Hengrui Medicine Co., Ltd., Jiangsu Prov., batch number: 13052125; Aniline blue, Beijing Lei Gen Bioisystech Co., Ltd, batch number: 0409A15; Yihong, Beijing Lei Gen Bioisystech Co., Ltd, batch number: 0409A15; DJ-1 antibody, U.S. santacruze; β-actin antibody, Shenyang all creation Bioisystech Co., Ltd; The goat antirabbit two of HRP labelling resists, Beijing Bioisystech Co., Ltd of Zhong Shan Golden Bridge; The super quick luminescent solution of ECL, SDS-PAGE gel prepare test kit, BCA protein quantification test kit, Beijing Suo Laibao Science and Technology Ltd.; SOD test kit, Bioengineering Research Institute is built up in Nanjing.
1.2 instrument
XL90 refrigerated centrifuge (German Beckman product), MK3 microplate reader (U.S. Thermo product), IX83 microscope (Japanese OLYMPUS product); Electrophresis apparatus, transferring film instrument, gel imaging system (U.S. bio-rad product).
1.3 animal
Male mice in kunming, 6-7 week age, provided by North China Polytechnics animal experimental center, credit number: SCXK(Ji) 2010-0038.Raise under constant temperature (21 ~ 23 DEG C), constant humidity (45% ~ 65%), each 12h light and shade periodic condition, ad lib and drinking-water.
experimental technique
2.1 integral experiment
2.1.1 the grouping of mice and administration
40 mices are divided into 4 groups at random, are respectively: matched group, model group and protocatechualdehyde are low, high dose group.Model group and protocatechualdehyde is low, high dose group intraperitoneal injection of cyclophosphamide solution 60mg/kg/d, continuous 5 days, matched group intraperitoneal injection of saline; Simultaneously low, the high dose group gavage of protocatechualdehyde give 10, the protocatechualdehyde solution of 40mg/kg/d, matched group and model group gavage give normal saline, totally 35 days.
2.1.2 mouse testis and epididymis assessment of indices
After last administration 24h, mouse weights is put to death, wins rapidly mice bilateral testes and epididymis, carefully reject peripheral adipose tissue and fascia tissue, weigh, calculate mouse testis and epididymis index.Testis index=testis weight/Mouse Weight, epididymis index=epididymis weight/Mouse Weight.
2.1.3 mouse sperm Nuclear maturity degree is evaluated
Take 0.05g aniline blue, add 20ml dehydrated alcohol and the dissolving of 80ml pure water, filter paper filtering, for subsequent use.Mice bilateral epididymal is placed in the normal saline of 37 DEG C of pre-temperature, wash away hair and blood, put into the small beaker filling 1ml normal saline, shredded by epididymis with eye scissors, static 3-5min, shakes gently.With four layers of lens papers filtration, suction strainer liquid smear, drying, dehydrated alcohol fixes 10min, dry, and aniline blue dyeing 7min, adds coverslip, and light microscopic 400 × observation after dry, mature sperm head is painted shallow, and non-mature sperm head is navy blue.Count the percentage rate of mature sperm in 200 sperms.
2.1.4 mouse testis morphological observation
Get mice left testes to fix in 10% neutral formalin, paraffin embedding, serial section, conventional H E dyes.Basis of microscopic observation is also taken pictures.
2.1.5 mouse testis SOD assay
Mouse testis tissue shreds, homogenate in normal saline, and 3000rpm4 DEG C of centrifugal 10min, gets supernatant, measures SOD activity and protein content in mouse testis tissue in strict accordance with test kit description.
2.1.6 mouse testis DJ-1 Protein Detection
Mouse testis tissue shreds, homogenate in RIPA lysate, and 12000rpm4 DEG C of centrifugal 20min, gets supernatant, measures mouse testis and organizes total protein content, determine albumen applied sample amount in strict accordance with test kit description.
According to test kit description configuration gel, protein sample adds 5 × sample-loading buffer, and 100 DEG C are boiled 5min, make protein denaturation.By protein quantification result loading, 50V constant voltage electrophoresis 0.5h, enters after concentrated glue until albumen and changes voltage, 100V constant voltage electrophoresis 2h.
After electrophoresis terminates, gel is placed in transferring film liquid and balances 30min, pvdf membrane activates 1min in methanol, according to " the positive glue of film is born " principle, glue and pvdf membrane is placed in membrane-transferring device.Use 200mA constant current electrophoresis 40min during transferring film, sample protein is transferred on pvdf membrane.
After transferring film terminates, pvdf membrane is placed in 5% defatted milk powder and closes 3h.
Add DJ-1 antibody (rabbit source, 1:500 dilutes), 4 DEG C of overnight incubation.Use TBST rinsing, 10min × 3, add the goat anti-rabbit igg (1:2000 dilution) of horseradish peroxidase-labeled, hatch 60min for 37 DEG C.Use TBST rinsing again, 10min × 3.
Add ECL luminescent solution, reaction 5min, gel imaging system imaging analysis.
2.2 experiment in vitro
2.2.1 the preparation of sperm suspension: get mice bilateral epididymal, be placed in the normal saline of 37 DEG C of pre-temperature, wash away hair and blood, epididymis is put into in the EP pipe of 1.5ml normal saline, eye scissors shreds, and after sperm is free, is filtered by supernatant 4 layers of lens paper, counting chamber counts, and cell concentration is adjusted to 2 × 10
7/ ml, 37 DEG C for subsequent use.
2.2.2 eosin stains detects living spermatozoa percentage: take 0.5g Eosin Y, add 100ml normal saline, fully dissolve, and filters.Sperm suspension is equally divided into 4 groups, matched group adds normal saline, and the basic, normal, high concentration group of protocatechualdehyde adds protocatechualdehyde, make its concentration be respectively 0.01,0.1,1mg/ml, 0.5h is hatched in 37 DEG C of water-baths.Get the sperm suspension after process and each 1 of Yihong solution, mixing, drips on microscope slide, adds coverslip, places 30 ~ 60s, light microscopic 400 × observation, and Necrospermia head is colored pinkiness, and sperm of living is not colored.Count 200 sperms, calculate living spermatozoa percentage.
2.2.3 sperm hypotonic expansion (hypo-osmoticswelling, HOS) experiment detects the integrity of sperm membrane: take 0.3625g sodium citrate, 0.675g fructose adds pure water to 50ml, is mixed with ionic strength 0.15, the hypotonic medium of osmotic pressure 150mOsm.Sperm suspension is equally divided into 4 groups, matched group adds normal saline, and the basic, normal, high concentration group of protocatechualdehyde adds protocatechualdehyde, make its concentration be respectively 0.01,0.1,1mg/ml, often group adds hypotonic medium 0.8ml, 37 DEG C of water-bath 0.5h.Draw precipitate after water-bath to drop on clean microscope slide, add coverslip humidity strip mounting, light microscopic 400 × observation, what sperm tail shape changed is swelling sperm, and the afterbody swelling sperm count counted in 200 sperms is overall expansion rate.
2.3 date processing
Experimental data all with
± s represents, carries out statistical test with ANOVA, compares P<0.05 and be considered as difference and have statistical significance between group.
experimental result and discussion
3.1 protocatechualdehyde are on the impact of mouse testis index, epididymis index and Maturation of sperm nucleus
Model group mouse testis index, epididymis index obviously reduce (
p< 0.01), and protocatechualdehyde can obviously increase the testis index of mice and epididymis index (
p< 0.05).In addition model group mouse sperm Nuclear maturity degree significantly decline (
p< 0.01), protocatechualdehyde can significantly improve mouse sperm Nuclear maturity degree (
p< 0.01), the results detailed in Table 1.
Testis is spermatogenetic vitals, epididymis is the place of spermioteleosis, after intraperitoneal injection of cyclophosphamide, mouse testis index and epididymis index reduce, and illustrate that mouse testis and epididymis sustain damage, and prompting mouse sperm generating process is impaired, spermioteleosis degree reduces; After protocatechualdehyde administration, mouse testis and epididymis index all increase, and illustrate that protocatechualdehyde can promote the reparation of impaired testis and epididymis.
In spermatogenesis, the albumen that sperm nucleus DNA combines can experience the natural maturity process from histone to protamine, and this is crossed and is called male sterility, and this group of type conversion has important physiological significance.Nucleoprotein group type translation exception, then immature sperm increases, and can produce a large amount of oxygen-derived free radicals, destroys sperm membrane, sperm membrane permeability is changed, thus causes dead sperm, and then affects male fertility, also can cause the embryonic loss of becoming pregnant.Aniline blue Coloration experiment shows, and model group mouse sperm Nuclear maturity degree obviously reduces, and prompting cyclophosphamide can cause male sterility abnormal, and then mice fertility may be made to reduce; And protocatechualdehyde can improve mouse sperm Nuclear maturity degree, prompting protocatechualdehyde can improve the fertility of male mice.
3.2 protocatechualdehyde are on the morphologic impact of mouse testis
Histology (with reference to Fig. 1) display of mouse testis, model group mice seminiferous tubule epithelium is thinning, and lumen diameter increases, and spermatogenic cell arrangement disorder, the spermatogenic cell number of plies reduce, oligospermia in tube chamber, and seminiferous tubule gap obviously increases; Protocatechualdehyde high dose group mouse testis form is similar to normal group, between each seminiferous tubule, arrangement closely, cell number of plies showed increased on seminiferous tubule spermatogenesis, each phase spermatogenic cell marshalling is closely orderly, the sperm that tube chamber is more as seen, prompting protocatechualdehyde can improve and recover spermatogenic epithelium damage, makes seminiferous tubule structure recovery normal, thus makes spermatogenesis recover normal.
Fig. 1 is protocatechualdehyde causes the Testis Morphology of chmice in male sterile impact (× 400) on cyclophosphamide, in figure: a: normal group, and b: model group, c: protocatechualdehyde low dose group (10mg/kg), d: protocatechualdehyde high dose group (40mg/kg).
3.3 protocatechualdehyde are on the impact of mouse testis tissue SOD activity
Compared with normal group, the active obviously reduction of model group mouse testis tissue SOD (
p< 0.01), the activity of protocatechualdehyde high dose group mouse testis tissue SOD significantly increases (
p< 0.05, with reference to Fig. 2).
Fig. 2 be protocatechualdehyde on cyclophosphamide cause chmice in male sterile testis tissue SOD activity impact (
± S, n=10), in figure: compared with Normal group,
## p<0.01; Compared with model group,
* p<0.05.
Research is had to think, the mechanism that cyclophosphamide causes spermatogenesis infringement may be that cyclophosphamide generates poisonous electrophilic material through the metabolism of Liver microsome P450 enzyme system, these electrophilic products cause free radical scavenging enzymatic activity to decline, free radical is caused to accumulate, affect the activity of steroid hormone synzyme, and lipid peroxidation occurs, biofilm structure is destroyed, cause Damage of Germ Cells, sperm microenvironment is changed.SOD is the enzyme-specific of the oxygen-derived free radicals such as cell clearance ultra-oxygen anion free radical and hydrogen peroxide.In this experiment, model group mice testis nine tissue SOD is active obviously declines, and hints model group mouse testis is subject to oxidativestress damage, thus affects spermatogenesis and sperm function.It is active that protocatechualdehyde can improve mouse testis tissue SOD, oxidative stress level in testis tissue reduced, thus recovers the spermatogenesis of mice.
3.4 protocatechualdehyde organize the impact of DJ-1 protein expression to mouse testis
Compared with normal group, model group mouse testis is organized DJ-1 to express obviously to reduce (
p< 0.01), protocatechualdehyde is low, high dose group mouse testis organizes DJ-1 to express significantly increases (
p< 0.05, with reference to Fig. 3).
Fig. 3 be protocatechualdehyde on cyclophosphamide cause impact that chmice in male sterile testis tissue DJ-1 expresses (
± S, n=3), in figure: compared with Normal group,
## p<0.01; Compared with model group,
* p<0.05,
* p<0.01.
DJ-1 albumen has obvious anti-oxidation stress function, expresses, all have expression at each phase spermatogenic cell of testis and Interstitial cell at male reproductive system height.Lot of documents shows, the buck that DJ-1 albumen and reproduction toxin cause is sterile closely related, and DJ-1 albumen is relevant to mankind's azoospermia also to have document to prove.The present invention finds, cyclophosphamide causes mouse testis to organize DJ-1 protein content obviously to reduce, and this can cause the anti-oxidation stress miopragia of DJ-1 albumen, and then testis tissue oxidative stress level is increased, thus affects spermatogenesis.And protocatechualdehyde can improve mouse testis and organizes DJ-1 protein content, thus antagonism testis tissue oxidative stress, make spermatogenesis recover normal, and then improve mice fertility.
3.4 protocatechualdehyde incubated in vitro are on the impact of living spermatozoa percentage
After normal saline and sperm suspension (matched group) hatch 0.5h living spermatozoa percentage be 21.58% ± 5.98%, 0.01,0.1,1mg/ml protocatechualdehyde and sperm suspension hatch, all can significantly improve living spermatozoa percentage, the results are shown in Table 2.
Sperm survival is the basis of sperm n-back test activity, and no matter in vivo or external, sperm certain hour of must surviving just can enter physiological change prefecundation such as hyperactivation state, experience capacitation.This research shows, and after protocatechualdehyde and mouse sperm are hatched, can significantly improve the Motility rate of mouse sperm, and prompting protocatechualdehyde can improve sperm function.
3.5 protocatechualdehyde incubated in vitro are on the impact of sperm membrane integrity
Matched group sperm overall expansion rate is 20.90% ± 3.38%, 0.01,0.1,1mg/ml protocatechualdehyde and sperm suspension hatch, sperm overall expansion rate can be significantly improved, the results are shown in Table 3.
Sperm tail is the organ of locomotion of sperm, and its function quality directly has influence on the energy of sperm.HOS is the simple and easy method measuring sperm uropatagium integrity and function.Sperm tail is more loose than head construction, therefore to hypotonic very responsive, can present various types of swelling at hypotonic environment.It is that sperm membrane shows normally that swelling occurs, therefore evaluates the film function of sperm mainly with this clinically.This research shows, and after protocatechualdehyde and mouse sperm are hatched, can significantly improve the percent of expansion sperm, and prompting protocatechualdehyde can protect sperm membrane integrity in vitro, improves sperm membrane function.
The advantage of protocatechualdehyde treatment male infertility and beneficial effect:
For male infertility, current clinical conventional medical treatment regime has: hormone therapy, comprises estrogen antagonist, promoting sexual gland hormone, gonadotropin releasing hormone, androgen, Aromatase inhibitor; Antioxidation Treatment, comprises conventional vitamin E, coenzyme Q10 etc.; Other drug, comprises carnitine etc.
(1) hormone therapy: estrogen antagonist curative effect is not affirmed, is not suitable for long-term treatment; The offer limited effectiveness of promoting sexual gland hormone, applies less; Costly, curative effect is not good enough for gonadotropin releasing hormone, generally not recommendation; Androgen in treating is to pregnancy rate and be not improved; Aromatase inhibitor can not improve semen quality, and expensive, therefore clinical less use.As can be seen here, hormone therapy is clinically and be of little use, and expensive; And protocatechualdehyde abundance, therefore relative low price, can promote the use of.
(2) Antioxidation Treatment: vitamin E curative effect is clearer and more definite, its mechanism of action mainly anti-oxidation stress, vitamin E is water insoluble, containing edible oil in preparation, treatment male infertility long-term taking Vitamin E preparation may cause patient's fat intake to increase, thus initiation relevant disease, as dyslipidemia etc.; And protocatechualdehyde good water solubility, patient's fat intake can not be increased.Coenzyme Q10 (400mg/d, 6 months) can improve patient's semen quality, and by drug price, patient's daily cost about 12 yuan, improves semen quality (6 months) expense about 2160 yuan; And protocatechualdehyde price is cheaper.The mechanism of action of vitamin E and coenzyme Q10 is antioxidation, and protocatechualdehyde is except to except anti-oxidation stress, also having other mechanism of action (increasing testis DJ-1 protein content).
(3) other: carnitine is applied more extensive at present, carnitine treatment male infertility common dose is 2-3g/d, with reference to drug price, patient daily cost 22-33 unit, 1 course for the treatment of (3 months) medical expense 1980-2970 unit, 2 course for the treatment of (6 months) medical expense 3960-5940 units, expense is relatively high; And protocatechualdehyde abundance, and every consumption per day few (300mg/d), therefore medical expense is lower than carnitine.The mechanism of action mainly antioxidation of carnitine, and protocatechualdehyde is except to except anti-oxidation stress, also having other mechanism of action (increasing testis DJ-1 protein content).
In sum, it is carnitine and vitamin E that current clinical treatment male infertility applies more medicine, the two main mechanism of action is anti-oxidation stress, protocatechualdehyde is except having anti-oxidative stress, testis DJ-1 protein content can also be improved, treatment male infertility mechanism is better than carnitine and vitamin E.Therefore, we think, protocatechualdehyde clinically can separately medication treatment male infertility, also can with carnitine and vitamin E conbined usage.
The advantage of protocatechualdehyde extracorporeal treatment sperm and beneficial effect:
Sperm nutrient solution conventional at present has Ham ' s-F10, Earle ' s, HTF etc., its main component is all various inorganic salt and aminoacid, does not add other compositions.In sperm nutrient solution, add protocatechualdehyde, its anti-oxidative stress can be played, remove the oxygen-derived free radicals produced in dealing sperm process, thus protection sperm morphology and function, than applying sperm nutrient solution effect more separately.In addition, protocatechualdehyde is cheap, can not increase the running cost of extracorporeal treatment sperm, and (directly adding in nutritional solution) easy to use, can not obviously increase operating procedure and time.
Claims (5)
1. the application of protocatechualdehyde in treatment male infertility and extracorporeal treatment sperm, is characterized in that protocatechualdehyde being used for the treatment of male infertility and extracorporeal treatment sperm.
2. the application of protocatechualdehyde according to claim 1 in treatment male infertility and extracorporeal treatment sperm, is characterized in that described protocatechualdehyde is used for the treatment of male infertility, uses solid preparation;
Dosage: 4.4mg/kg(is about 300mg/d);
Administrated method: oral.
3. drug treatment: a 1-2 spermatogenic cycle, namely 3-6 month;
Can be used alone, also can share with other medicines.
4. the application of protocatechualdehyde according to claim 1 in treatment male infertility and extracorporeal treatment sperm, is characterized in that described protocatechualdehyde is for the male infertility of preventing and treating cyclophosphamide and causing and Reproductive Damage.
5. the application of protocatechualdehyde according to claim 1 in treatment male infertility and extracorporeal treatment sperm, it is characterized in that described protocatechualdehyde is for extracorporeal treatment sperm, make injectable powder, matching while using, after a small amount of physiological saline solution, add in sperm nutrient solution and use, concentration is 0.1mg/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510596135.3A CN105250246B (en) | 2015-09-18 | 2015-09-18 | Application of the protocatechualdehyde in treatment male sterility and extracorporeal treatment sperm |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101788299B1 (en) | 2016-02-02 | 2017-10-19 | 부산대학교 산학협력단 | Composition containing protocatechuic acid for improving pregnancy |
| CN111675740A (en) * | 2020-05-18 | 2020-09-18 | 南通大学 | Potholin glucoside, application of pinosylin glucoside and preparation method of pinosylin glucoside |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2015134725A (en) * | 2014-01-16 | 2015-07-27 | 株式会社紀州ほそ川 | Therapeutic agent for infertility |
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| JP2015134725A (en) * | 2014-01-16 | 2015-07-27 | 株式会社紀州ほそ川 | Therapeutic agent for infertility |
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| 周乐 等: ""丹参水提物预防糖皮质激素对大鼠睾丸损害的实验研究"", 《广西医学》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101788299B1 (en) | 2016-02-02 | 2017-10-19 | 부산대학교 산학협력단 | Composition containing protocatechuic acid for improving pregnancy |
| CN111675740A (en) * | 2020-05-18 | 2020-09-18 | 南通大学 | Potholin glucoside, application of pinosylin glucoside and preparation method of pinosylin glucoside |
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| CN105250246B (en) | 2017-12-15 |
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