CN105248333A - Method of assessing shellfish health condition by adopting dynamometer - Google Patents
Method of assessing shellfish health condition by adopting dynamometer Download PDFInfo
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- CN105248333A CN105248333A CN201510769453.5A CN201510769453A CN105248333A CN 105248333 A CN105248333 A CN 105248333A CN 201510769453 A CN201510769453 A CN 201510769453A CN 105248333 A CN105248333 A CN 105248333A
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- byssus
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- 235000015170 shellfish Nutrition 0.000 title claims abstract description 92
- 238000000034 method Methods 0.000 title claims abstract description 40
- 230000036541 health Effects 0.000 title abstract description 6
- 238000012360 testing method Methods 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 230000008859 change Effects 0.000 claims abstract description 4
- 230000003862 health status Effects 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 6
- 230000000712 assembly Effects 0.000 abstract 1
- 238000000429 assembly Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 230000007423 decrease Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000012214 genetic breeding Methods 0.000 description 3
- 241000237519 Bivalvia Species 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 235000020639 clam Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 241000237536 Mytilus edulis Species 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 108010006161 conchiolin Proteins 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009318 large scale farming Methods 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 235000020638 mussel Nutrition 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000036387 respiratory rate Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000009278 visceral effect Effects 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
Abstract
The invention relates to a method of assessing a shellfish health condition by adopting a dynamometer, comprising the following steps: selecting a proper shellfish with a certain specification; performing treatment on the shellfish before shell opening; opening the shell of the shellfish by adopting a shell opener; connecting various assemblies of the LH-F490 portable dynamometer; starting the LH-F490 portable dynamometer and testing, i.e., setting parameters after starting, pressing a zero clearing key for zero clearing, and testing whether the display pressure change of a displayer exists or not, wherein a tension sensor is selected as a clamp of the LH-F490 portable dynamometer, displayed data N1 is recorded, a byssus of the shellfish is taken out, vertically penetrates through a test ring, and is pulled apart, and displayed data N2 is recorded; an occlusal force sensor is selected as the clamp of the LH-F490 portable dynamometer, displayed data N3 is recorded, a contact of the clamp is clamped in a shell, and displayed data N4 is recorded; putting the tested shellfish in another identical water environment for continuously culturing. The method has the advantages that high speed and convenience are realized, mechanical damage conditions are reduced, and the method can be widely applied to a shellfish culture process.
Description
Technical field
The present invention relates to shellfish health status technical field, specifically, is a kind of method adopting dynamometer to assess shellfish health status.
Background technology
Along with social development, the expansion water treatment of the water pollution that modernization development brings and aquaculture is perfect not, and current Natural Water environmental complex situation is not very optimistic.Water quality administer and cultivation specification very important while, cultivate resistance improved seeds and seem quite important.But in breeding process, except the requirement of parent's quality and cultural technique, the detection of the daily health status to shellfish is the key factor cultivating improved seeds.Although routine testing comprises survey the physiological status that respiratory rate, digestibility, rate of body weight gain etc. index more precisely can express shellfish, but concerning large-scale industrialized cultivation, method that is quick, convenient, time controlled check and evaluation shellfish health status is undoubtedly significantly.
Shellfish, belongs to the lamellibranchiata (or Bivalvia) in Mollusca.Mobility is more weak, has generally externally draped over one's shoulders 1-2 block shell.Common oyster, mussel, clam, razor clam etc. all belong to this type of.Their outer embrane is coated in the outside of health, and its epidermal cell secretion shell, outer embrane and shell are all the armours of shellfish.Shell, by closed shell flesh and ligament, controls the closed of shellfish and opens.The stranded power surveying shellfish is equivalent to the neural reflex immunity measuring shellfish.
Byssus is that the slightly ectocentral byssal foramen of lamellibranch foot stretches out, and take conchiolin as the obdurability fiber bundle of the scleroprotein of main component.Whereby shellfish body is bonded on rock or marine alga and other animal body.Therefore, shellfish enter into a new environment for set oneself and the toughness of oozy byssus also can compare and get information about shellfish own physiological situation and an adaptation situation to residing environment.
Existing routine normally contains chemical combination material, toxin etc. by analysis of experiments shellfish to shellfish health operations mode to be assessed, and this has the following disadvantages and defect, and 1, loaded down with trivial details to shellfish testing process, and easily to damaging in shellfish; 2, long to the shellfish detection experiment time, and the time is uncontrollable, costly; 3. be not suitable for large-scale farming, add seed selection workload; 4, intuitively can not reflect the physiological situation of shellfish and an adaptation situation to residing environment, understand the adaptability of the current activity of shellfish and the environment to existence, can not provide support to the seed selection work of shellfish genetic breeding.
In sum, a kind of method of quick, convenient, employing dynamometer that the time is controlled assessment shellfish health status is needed badly.And yet there are no report about this method.
Summary of the invention
The object of the invention is for deficiency of the prior art, a kind of method of quick, convenient, employing dynamometer that the time is controlled assessment shellfish health status is provided.
For achieving the above object, the technical scheme that the present invention takes is:
Adopt dynamometer to assess a method for shellfish health status, comprise the following steps:
Step S1, departs from shellfish outside water environment and makes to detect by an unaided eye, and randomly draws quick-make after shellfish is closure state or touching, selects suitable and have the shellfish of certain specification, described shellfish specification be shell length at more than 7cm, shell keeps complete in addition;
Step S2, operating personnel put on one's gloves and open shell pre-treatment to shellfish, clean shellfish a little, just observe the reference factor as a result such as glossiness, byssus number of shell, and cut byssus;
Step S3, adopt cracker to shellfish opening, one holds cracker handle, makes flat tip along shell opening part and firmly inwardly inserts, holding certain power, opens shell shell is opened opening that width is 1.4-1.6cm;
Step S4, connect each assembly of LH-F490 portable force gauge, comprise display and sensor, display components has by mechanical axis, tuning block, LCDs, reset key, power supply indicator, power key, return key, reset key, upwards options button, downwards options button, the key that moves to left, the key that moves to right, acknowledgement key, USB joint, RS232 serial ports, outlet, power supply adaptor; Sensor is made up of sensing joint, sense line, pulling force receptor or snap-in force receptor, test wrapper;
Step S5, LH-F490 portable force gauge is started shooting and is tested, and sets parameter after start, presses reset key and resets, and tests whether display has the change of display pressure;
The fixture of step S6, LH-F490 portable force gauge selects pulling force receptor, presses reset key and resets, and record display data N1, takes out a rhizopodium silk of shellfish, pass perpendicularly through test wrapper, pull apart byssus, record display data N2;
The fixture of step S7, LH-F490 portable force gauge selects snap-in force receptor, presses reset key and resets, and record display data N3, makes the contact of fixture be clipped between shell, after stable, and record force value N4;
Step S8, places surveyed shellfish in other identical water environment and continues cultivation.
Preferably, in described step S3, described cracker material uses stainless steel, cracker comprises handle, rotating shaft, flat tip three part, band is carved with at handle place, rotating shaft is mutually chimeric and fixed through spring by a short fulcrum at axle eye place, flat tip edge arc-shaped and sharp, flat tip place plane and two handle place plane orthogonal.
Preferably, in described step S3, described flat tip inserts the shell degree of depth and is less than 0.5cm.
Preferably, in described step S3, it is 1.5cm that described shell opens width.
Preferably, the described RS232 serial ports way of output only controls the transmission of real-time testing data.
Preferably, described parameters is pressed acknowledgement key and is entered the parameter arranging required amendment under test interface.
Preferably, described byssus takes out three from sufficient amount and certain length, measures N1.1, N1.2, N1.3 respectively, results averaged, and the toughness calculating byssus is the reflection of the value of IN2I-IN1I.
Preferably, the direction of described fixture contact is contrary with the snap-in force direction of shell, and its stranded power calculating shell is the value of IN4I-IN3I.
Preferably, the contact of described fixture upwards.
The invention has the advantages that:
1, a kind of method adopting dynamometer to assess shellfish health status of the present invention, shellfish that is loaded down with trivial details and that have compared to the health operations mode of conventional detection shellfish detects and easily damages shellfish, method of the present invention is quick, convenient, mechanical damage situation lowers, and can be widely used in the middle of shellfish culture process; 2, LH-F490 dynamometer is utilized to estimate the shellfish health status method particularly stranded power of shell flesh and byssus toughness, and then understand the adaptability of the current activity of shellfish and the environment to existence, the seed selection work of shellfish genetic breeding is provided support, decreases the loaded down with trivial details of the active mode of operation of conventional detection shellfish and shellfish is damaged;
3, simple to operate, convenient, understandable, be applicable to just get started scholar;
4, quick, convenient, time of a large amount of detection experiment and expense in less a lot of production;
5, reduce the damage of shellfish, reduce water quality artificial pollution, increase economic efficiency further;
6, rough estimate, reduces range of choice, decreases seed selection workload.
Accompanying drawing explanation
Accompanying drawing 1 is a kind of method flow schematic diagram adopting dynamometer to assess shellfish health status of the present invention.
Accompanying drawing 2 cracker constructs and opens shell schematic diagram.
Shell schematic diagram opened by accompanying drawing 3 pulling force receptor.
Shell schematic diagram opened by accompanying drawing 4 snap-in force receptor.
Embodiment
Below in conjunction with accompanying drawing, embodiment provided by the invention is elaborated.
The Reference numeral related in accompanying drawing and part as follows:
1. handle 2. rotating shaft
3. flat tip 4. shell opening part
5. pulling force receptor 6. test wrapper
7. byssus 8. sense line
9. sensing joint 10 display
11 snap-in force receptors
Please refer to Fig. 1, Fig. 1 is a kind of method flow schematic diagram adopting dynamometer to assess shellfish health status of the present invention.Adopt dynamometer to assess a method for shellfish health status, utilize active force and reaction force principle indirectly to reflect estimation method and achieve the goal in conjunction with electronic sensor.When the sense organ of shellfish experiences external force, shellfish will start defense mechanism, retraction abdominal foot and the shell that closes, now snap-in force receptor 11 contact is sandwiched between shell along opening, make contact upward and contrary with shell snap-in force direction and on same application point, active force is identical with reaction force size, is shown to display 10 by sensor passes.The method is scientific and reasonable, with the physiological situation of the reflection of power trickle displaying shellfish directly perceived.Said method comprising the steps of:
Step S1, selects the suitable shellfish with having certain specification.Shellfish is departed from outside water environment and makes to detect by an unaided eye, randomly draw shellfish be closure state or touching after quick-make.Shell keeps complete in addition, and shell length is at more than 7cm.Too little shellfish resistance is relatively poor, and snap-in force is relatively little, not easily measures.
Step S2, opens shell pre-treatment to shellfish.Before shellfish opening, operating personnel bring gloves in order to avoid misoperation is injured; Then clean shellfish a little, just observe the reference factor as a result such as glossiness, byssus 7 number of shell; Finally cut byssus 7.
Step S3, adopts special cracker to shellfish opening.
Please refer to Fig. 2, Fig. 2 is cracker structure and opens shell schematic diagram.Cracker material uses stainless steel, comprises handle 1, rotating shaft 2, flat tip 3 three part.Band is carved with, for increasing frictional force in handle 1 place.Rotating shaft 2 is mutually chimeric and fixed through spring by a short fulcrum at axle eye place.Flat tip 3 edge arc-shaped and sharp, flat tip 3 place plane and two handle 1 place plane orthogonal.When using cracker, one holds cracker handle, make flat tip 3 along shell opening part 4 and firmly inwardly insert, hold certain power, open shell shell is opened opening that width is about 1.4-1.6cm, it is 1.5cm that preferred shell opens width, prevent shellfish closed shell flesh and other positions injured.Flat tip 3 inserts shell need be less than 0.5cm deeply, crosses dark easy damaged outer embrane and visceral mass.
Step S4, connects each assembly of LH-F490 portable force gauge, comprises display 10 and sensor.Display 10 parts have by mechanical axis, tuning block, LCDs, reset key, power supply indicator, power key, return key, reset key, upwards options button, downwards options button, the key that moves to left, the key that moves to right, acknowledgement key, USB joint, RS232 serial ports, outlet, power supply adaptor; Sensor is made up of sensing joint 9, sense line 8, pulling force receptor 5 (see Fig. 3) or snap-in force receptor 11 (see Fig. 4).Test wrapper 6 and sensor all can not too firmly go to twist when installing.Wherein, RS232 serial ports, its way of output only controls the transmission of real-time testing data.
Step S5, LH-F490 portable force gauge is started shooting and is tested.Set parameter after start, press reset key and reset, test whether display 10 has the change of display pressure.It should be noted that: parameters is pressed acknowledgement key and entered the parameter arranging required amendment under test interface.
The fixture of step S6, LH-F490 portable force gauge selects pulling force receptor 5.Press reset key to reset, record display data N1, byssus 7 takes out three (having the byssus 7 of enough numbers), passes perpendicularly through test wrapper 6, pulls apart byssus 7, measure N1.1, N1.2, N1.3 respectively, results averaged, record display data N2.The toughness calculating byssus 7 is the reflection of the value of IN2I-IN1I.
The fixture of step S7, LH-F490 portable force gauge selects snap-in force receptor 11.Press reset key to reset, record display data N3, make the contact of fixture be clipped between shell, the direction of fixture contact is contrary with the stranded force direction of shell.The contact of fixture upwards, measure downwards come value forbidden, easily slip off or the direction of power not right.After stable, record force value N4.Its snap-in force calculating shell is the value of IN4I-IN3I.
Step S8, places surveyed shellfish in other identical water environment and continues cultivation.Prevent the injured death of shellfish that in testing process, some immunity is weak, and then affect water quality.And similar water environment can make shellfish adapt to sooner and repair fast.
A kind of method adopting dynamometer to assess shellfish health status of the present invention, shellfish that is loaded down with trivial details and that have compared to the health operations mode of conventional detection shellfish detects and easily damages shellfish, method of the present invention is quick, convenient, mechanical damage situation lowers, and can be widely used in the middle of shellfish culture process; LH-F490 dynamometer is utilized to estimate the shellfish health status method particularly stranded power of shell flesh and byssus 7 toughness, and then understand the adaptability of the current activity of shellfish and the environment to existence, the seed selection work of shellfish genetic breeding is provided support, decreases the loaded down with trivial details of the active mode of operation of conventional detection shellfish and shellfish is damaged; Simple to operate, convenient, understandable, be applicable to just get started scholar; Fast, convenient, time of a large amount of detection experiment and expense in less a lot of production; Reduce the damage of shellfish, reduce water quality artificial pollution, increase economic efficiency further; Rough estimate, reduces range of choice, decreases seed selection workload.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the inventive method; can also make some improvement and supplement, these improve and supplement and also should be considered as protection scope of the present invention.
Claims (9)
1. adopt dynamometer to assess a method for shellfish health status, it is characterized in that, comprise the following steps:
Step S1, departs from shellfish outside water environment and makes to detect by an unaided eye, and randomly draws quick-make after shellfish is closure state or touching, selects suitable and have the shellfish of certain specification, described shellfish specification be shell length at more than 7cm, shell keeps complete in addition;
Step S2, operating personnel put on one's gloves and open shell pre-treatment to shellfish, clean shellfish a little, just observe the reference factor as a result such as glossiness, byssus number of shell, and cut byssus;
Step S3, adopt cracker to shellfish opening, one holds cracker handle, makes flat tip along shell opening part and firmly inwardly inserts, holding certain power, opens shell shell is opened opening that width is 1.4-1.6cm;
Step S4, connect each assembly of LH-F490 portable force gauge, comprise display and sensor, display components has by mechanical axis, tuning block, LCDs, reset key, power supply indicator, power key, return key, reset key, upwards options button, downwards options button, the key that moves to left, the key that moves to right, acknowledgement key, USB joint, RS232 serial ports, outlet, power supply adaptor; Sensor is made up of sensing joint, sense line, pulling force receptor or snap-in force receptor, test wrapper;
Step S5, LH-F490 portable force gauge is started shooting and is tested, and sets parameter after start, presses reset key and resets, and tests whether display has the change of display pressure;
The fixture of step S6, LH-F490 portable force gauge selects pulling force receptor, presses reset key and resets, and record display data N1, takes out a rhizopodium silk of shellfish, pass perpendicularly through test wrapper, pull apart byssus, record display data N2;
The fixture of step S7, LH-F490 portable force gauge selects snap-in force receptor, presses reset key and resets, and record display data N3, makes the contact of fixture be clipped between shell, after stable, and record force value N4;
Step S8, places surveyed shellfish in other identical water environment and continues cultivation.
2. method according to claim 1, it is characterized in that, in described step S3, described cracker material uses stainless steel, cracker comprises handle, rotating shaft, flat tip three part, and band is carved with at handle place, and rotating shaft is mutually chimeric and fixed through spring by a short fulcrum at axle eye place, flat tip edge arc-shaped and sharp, flat tip place plane and two handle place plane orthogonal.
3. method according to claim 1, is characterized in that, in described step S3, described flat tip inserts the shell degree of depth and is less than 0.5cm.
4. method according to claim 1, is characterized in that, in described step S3, it is 1.5cm that described shell opens width.
5. method according to claim 1, is characterized in that, in described step S4, the described RS232 serial ports way of output only controls the transmission of real-time testing data.
6. method according to claim 1, is characterized in that, in described step S5, described parameters is pressed acknowledgement key and entered the parameter arranging required amendment under test interface.
7. method according to claim 1, is characterized in that, in described step S6, described byssus takes out three from sufficient amount and certain length, measure N1.1, N1.2, N1.3 respectively, results averaged, the toughness calculating byssus is the reflection of the value of IN2I-IN1I.
8. method according to claim 1, is characterized in that, in described step S7, the direction of described fixture contact is contrary with the snap-in force direction of shell, and its stranded power calculating shell is the value of IN4I-IN3I.
9. method according to claim 1, is characterized in that, in described step S8, the contact of described fixture upwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510769453.5A CN105248333B (en) | 2015-11-12 | 2015-11-12 | A kind of method that shellfish health status is assessed using dynamometer |
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| CN201510769453.5A CN105248333B (en) | 2015-11-12 | 2015-11-12 | A kind of method that shellfish health status is assessed using dynamometer |
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| CN105248333A true CN105248333A (en) | 2016-01-20 |
| CN105248333B CN105248333B (en) | 2018-01-09 |
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| CN111202018A (en) * | 2020-03-17 | 2020-05-29 | 鲁东大学 | Improved measuring device for tension of adductor muscle of bivalve shellfish |
| CN114642186A (en) * | 2022-03-29 | 2022-06-21 | 中国农业大学 | Activity detection device and method in oyster keep-alive transportation process |
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| CN114642186B (en) * | 2022-03-29 | 2023-03-07 | 中国农业大学 | Activity detection device and method in oyster keep-alive transportation process |
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| Publication number | Publication date |
|---|---|
| CN105248333B (en) | 2018-01-09 |
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