CN105238098B - A kind of purple corn cob anthocyanin dynamic gradient embedding method - Google Patents
A kind of purple corn cob anthocyanin dynamic gradient embedding method Download PDFInfo
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Abstract
The present invention is to provide a kind of purple corn cob anthocyanin dynamic gradient embedding method.Using octenyl succinic acid anhydride modified gum arabic, maltodextrin and hydroxypropyl beta cyclodextrin as wall material, using water extraction and the purple corn cob anthocyanin through macroporous resin purification is core, to improve anthocyanin embedding efficiency, embedding yield and storage stability, the present invention uses dynamic gradient investment fashion, wall material concentration is controlled from low to high, so as to realize the multiple embedding to anthocyanin.After measured, the embedding efficiency of purple corn cob anthocyanin microcapsules up to 94 99%, embedding yield is up to 92 96%.Compared with whole isoconcentration investment, embedding efficiency improves 20% 35%, and embedding yield improves 25 40%.Purple corn cob anthocyanin after gradient embedding is after 60 70d under the conditions of normal-temperature light photograph, and its storage rate can reach more than 90%, and compared with whole isoconcentration investment, illumination stabilization time improves 500 600%.
Description
Technical field
The present invention relates to cereal intensive processing technical field, and in particular to a kind of purple corn cob anthocyanidin embedding method, it is special
It is not related to embedding efficiency, embedding yield and storage stability that a kind of dynamic gradient embedding method improves purple corn cob anthocyanidin.
Background technology
Anthocyanin is widely distributed, it is known that the anthocyanin in nature just has kind more than 600.Anthocyanin have than vitamin C and
The higher oxidation resistance of the common antioxidant such as vitamin E, remove free radical, antibacterial, anti-inflammatory and suppress growth of cancer cells
Ability is strong, and anthocyanin class material is alternatively arranged as natural additive for foodstuff, the coloring for food such as cold drink, jelly, candies.
Therefore, food, cosmetic, nutrition and the common concern of pharmaceutical field scholar are enjoyed.
At present, the research about purple corn cob and anthocyanin is for example following：
The extraction of the Purple Corn Anthocyanins and isolate and purify aspect, a kind of purple maize leaves are disclosed in 201110194836.6
The extraction process of anthocyanidin, it the advantages of be by ultrasonic assistant extraction method improve anthocyanidin recovery rate, but ultrasound at
Reason may cause the change of anthocyanin structure, and the technique does not carry out removal of impurities processing to anthocyanin, to the stability of anthocyanin
Not specified (NS).The other technique is not directed to purple corn cob using purple maize leaves as raw material.One is disclosed in 201310673454.0
The extraction process of kind of the tasteless corn pigment of high stability, it the advantages of be to extract by ultrasonic assistant, zymolysis technique removal of impurities,
Macroporous resin adsorption and membrane separation technique deodorization and purifying, and tasteless, fast light and heat-resisting the Purple Corn Anthocyanins have been finally given,
But the time unstable to illumination explains in the stability experiment in the invention, and the invention is using purple maize peel as raw material, not
It is related to purple corn cob.Similar with the invention also has in a kind of purple corn pigment extraction process disclosed in 201310670696.4.
The preparation method of C-3-G is disclosed in 201110137741.0, it the advantages of be to enter anthocyanin
The extracting and developing and carrying out of having gone finishes structure identification, but this method does not explain to the stability of anthocyanin, in addition, this method with
Purple crop is raw material, not more particularly to purple corn cob.
Relevant flower color glycosides stability study technical elements, disclose a kind of purple corn cob in 201110225754.3
The method of the auxiliary color of anthocyanin, it the advantages of be by the use of glycine as auxiliary toner to improve the thermostabilization of purple corn cob anthocyanin
Property, and reduce influence of the metal ion to purple corn cob anthocyanin stability.But this method is not directed to purple corn cob anthocyanin
Key is stable to answer influence factor, i.e. light durability.A kind of system of anthocyanin lipidosome is disclosed in 201310413928.8
Preparation Method, it is to embed anthocyanidin by preparing the method for anthocyanin lipidosome suspension and be freeze-dried the advantages of this method
Anthocyanin lipidosome, but the freeze-drying high energy consumption that this method uses are obtained, drying time length, is not suitable for industrialized production.
Disclose a kind of OPC spansule in 02117320.6, be with calcium alginate, chitosan etc. the advantages of this method
Macromolecular realizes multilayer embedding OPC and prepares OPC spansule using orifice method, to reach the former flower of slowly release
The effect of blue or green element, but this method is not directed to the stability of anthocyanidin, in addition, orifice method is high not as good as spray drying process production efficiency.
Disclose a kind of blackberry anthocyanin microcapsules and preparation method thereof in 201110440469.3, be the advantages of this method using Ah
Draw the macromolecular wall materials such as primary glue and maltodextrin embed anthocyanin and by spray drying prepare the methods of anthocyanin microcapsules with
Anthocyanin stability is improved, but the Arabic gum amount that this method uses is big, and Arabic gum is costly；Secondly, this method palpus
By control feed liquid concentration and addition anticaking agent to solve the adhesion problem occurred in spray-drying process, process is more complicated；
Furthermore this method, which is not investigated, uses gradient concentration embedding to improve embedding efficiency and yield, and this method is using blackberry, blueberry as raw material, not
It is related to purple corn cob.Similar open invention is additionally included in the manufacture method of the purple corn anthocyanin disclosed in 200710064006
With a kind of oligomeric proanhocyanidins microcapsule disclosed in 200720154938.4.
In terms of research about purple corn cob, what invention disclosed was related to is its application in the industry, such as
Purple corn beverage disclosed in 200810119985 and preparation method thereof, a kind of purple corn bag disclosed in 200910010934.2
Make tea and preparation method thereof, the corn core flour microorganism control method disclosed in 200910136137.9,200910136138.3
Disclosed in corn core carrier modifying method, a kind of maize cob meal disclosed in 201110057680.7 etc..Though these inventions are all
Using corncob or purple corn cob as raw material, but it is the stability study for being related to wherein anthocyanin.
For anthocyanin as a kind of functional materials, its less stable is sensitive to factors such as light, heat and pH, limits it
Application in the industry, therefore anthocyanin stability study turns into anthocyanin area research focus.Such as above-mentioned partial monopoly document
Disclosed is the current common method for improving anthocyanin stability using auxiliary toner or the method for embedding, wherein, investment is the most
It is common, but above-mentioned patent document do not investigate repeatedly embedded using gradient concentration to anthocyanin embedding efficiency, embedding yield and
The influence of anthocyanin stability, also without open anthocyanin gradient embedding method；In addition, it is common to use Arabic gum concentration
It is high so that cost of material is significantly increased；Furthermore feed concentration or addition anticaking agent must be controlled to avoid bonding from asking during spray drying
Topic, process are complicated.
In terms of anthocyanin embedding techniques, at present, most of patent documents are with blackberry, blueberry, blueberry, purple potato etc. disclosed in the country
For research object, have no that the patent document of purple corn cob embedding discloses.Purple corn cob is the accessory substance after purple corn processing, and it is rich
The anthocyanin class material contained can effectively remove free radical, prevent ultraviolet radiation, be supported available for antitumor, anti-aging and skin care
Face.Purple corn cob abundance, cost is low, and with it is common rich in the plant material of anthocyanin compared with, not by seasonal effect.
Based on the function of purple corn cob anthocyanin, the method embedded by gradient concentration improves the embedding effect of anthocyanin
Rate, embedding yield and storage stability, reduce cost, it is necessary to design a set of new production method bag according to food processing principle
Bury purple corn cob anthocyanin.
The content of the invention
The present invention is in view of the deficiencies of the prior art, there is provided a kind of purple corn cob anthocyanin dynamic gradient embedding method.
It is an object of the invention to provide a kind of purple corn cob anthocyanin dynamic gradient embedding method, specifically with octene
The succinic anhydride modified Arabic gum of base, maltodextrin and hydroxypropylβ-cyclodextrin are wall material, with water extraction and pure through macroreticular resin
The purple corn cob anthocyanin of change is core, and using dynamic gradient investment fashion, control wall material concentration from low to high, is realized to pattern
The multiple embedding of glycosides, anthocyanin embedding efficiency, embedding yield and storage stability are improved, while reduce cost, and realized direct
It is spray-dried and feed concentration need not be controlled, simplifies technique.A kind of purple corn cob anthocyanin dynamic gradient bag of the present invention
Method is buried to realize by following technical proposals and step：
Step 1, the preparation of wall material, it is respectively 100-130g/L, 140-170g/L, 180- with distilled water compound concentration
210g/L colloid wall material solution；Wherein, every liter of colloid wall material solution contains 20-40g octenyl succinic acid anhydride unmodified gums
Glue, 40-100g maltodextrins, 40-100g hydroxypropylβ-cyclodextrins, 0.05-0.20g EDTA-2Na；
Step 2, the preparation of core, using 80% ethanol solution containing 1% citric acid as extractant, purple corn cob powder with
The ratio of extractant is 1:8-1:10W/V, extracted 2 times at 55 DEG C, each extraction time is 60min, obtains thick purple corn cob
Anthocyanin solution a；HP20 macroreticular resins, HP20 macroreticular resins and thick purple corn cob are added in thick purple corn cob anthocyanin solution a
Anthocyanin solution a ratio is 1:40-1:70W/V, filter, then use after being placed in 25 DEG C of shaking bath 100r/min vibration absorption 5h
The anthocyanin of 95% ethanol solution elution resin adsorption, elution time 6-10h, eluent is after ethanol is removed in decompression with distillation
Water dissolves, and obtains purple corn cob anthocyanin solution b after purification；The dry weight content of anthocyanin in purple corn cob anthocyanin solution b
1.05-1.72g/100g；
Step 3, the gradient embedding of purple corn cob anthocyanin, 100-130g/L colloid wall material solution is added while stirring
Enter in purple corn cob anthocyanin solution b, wall material and core mass ratio are 0.5-0.75:1, stir 15-20min in 60 DEG C of constant temperature
Afterwards, 140-170g/L colloid wall material solution is added, wall material and core mass ratio are 0.75-1.0:1, stirred in 60 DEG C of constant temperature
After 15-20min, homogeneous 1 time under 40MPa pressure conditions, 180-210g/L colloid wall material solution, wall material and core material are added
It is 0.75-1.0 to measure ratio:Homogeneous 1 time under Isosorbide-5-Nitrae 0MPa pressure conditions, the purple corn cob anthocyanin compound after being embedded；
Step 4, dry, the purple corn cob anthocyanin compound after embedding is dried using spray drying process, EAT is
180 DEG C, leaving air temp is 85 DEG C, and compound concentration need not be controlled when drying.
The present invention has the advantage that compared with prior art：The present invention uses gradient investment, by stepping up wall
The mode of material concentration, it can be achieved to the multiple embedding for the anthocyanidin not embedded, so as to improve the embedding of purple corn cob anthocyanin effect
Rate and yield and storage stability.After measured, the embedding efficiency of purple corn cob anthocyanin microcapsules reaches 94-99%, embedding yield
Up to 92-96%.Compared with whole isoconcentration investment, embedding efficiency improves 20%-35%, and embedding yield improves 25-
40%.Purple corn cob anthocyanin after gradient embedding under the conditions of normal-temperature light photograph after 60-70d, its storage rate can reach 90% with
On, compared with whole isoconcentration investment, illumination stabilization time improves 500-600%.The present invention uses ocentyl succinic
Anhydride modification Arabic gum is as one of wall material raw material, and compared with unmodified gum glue, the modified gum arabic can obviously reduce
The addition of Arabic gum, so as to which embedding cost be greatly reduced；Meanwhile can to significantly improve wall material colloid molten for the modified gum arabic
The mobility and film-formation result of liquid, so as to realize high encapsulation efficiencies and yield under low consistency conditions；Furthermore wall material colloid is molten
Liquid has compared with low viscosity characteristics, can effectively be avoided in the case where not controlling spray drying feed concentration in spray-drying process
Feed liquid bonds, and reduces the moisture in dry products, improves product storage stability.
Embodiment
Embodiment 1：
(1) preparation of wall material is respectively that 100g/L, 140g/L, 180g/L colloid wall material are molten with distilled water compound concentration
Liquid.Wherein, every liter of 100g/L colloid wall material solution contain 20g octenyl succinic acid anhydrides modified gum arabic, 40g maltodextrins,
40g hydroxypropylβ-cyclodextrins, 0.05gEDTA-2Na；Every liter of 140g/L colloid wall material solution contains 20g octenyl succinic acid anhydrides and changed
Property Arabic gum, 60g maltodextrins, 60g hydroxypropylβ-cyclodextrins, 0.05gEDTA-2Na；Every liter of 180g/L colloid wall material solution
Contain 20g octenyl succinic acid anhydrides modified gum arabic, 80g maltodextrins, 80g hydroxypropylβ-cyclodextrins, 0.05gEDTA-
2Na；
(2) preparation of core is using 80% ethanol solution containing 1% citric acid as extractant, purple corn cob powder and extractant
Ratio be 1:8 (W/V), extracted 2 times at 55 DEG C, each extraction time is 60min, obtains thick purple corn cob anthocyanin solution
(a).Addition HP20 macroreticular resins in a, HP20 macroreticular resins and a ratio are 1:70 (W/V), it is placed in 25 DEG C of shaking baths
Filtered after 100r/min vibration absorption 5h, then the anthocyanin of the ethanol solution elution resin adsorption with 95%, elution time 6h,
Eluent is dissolved after ethanol is removed in decompression with distilled water, obtains purple corn cob anthocyanin solution (b) after purification.Anthocyanin in b
Content be 1.05g/100g (dry weight)；
(3) the gradient embedding of purple corn cob anthocyanin adds 100g/L colloid wall material solution in b solution while stirring,
Wall material is 0.5 with core mass ratio:1, after 60 DEG C of constant temperature stir 15min, add 140g/L colloid wall material solution, wall material with
Core mass ratio is 0.75:1, after 60 DEG C of constant temperature stir 20min, homogeneous 1 time, adds 180g/L under 40MPa pressure conditions
Colloid wall material solution, wall material and core mass ratio are 0.75:Homogeneous 1 time, the purple after being embedded under Isosorbide-5-Nitrae 0MPa pressure conditions
Corncob anthocyanin compound.Compared with 180g/L colloid wall material solution whole process isoconcentration embedding.
(4) dry and the purple corn cob anthocyanin compound after embedding, EAT 180 are dried using spray drying process
DEG C, leaving air temp is 85 DEG C, and compound concentration need not be controlled when drying.
Embodiment 2：
(1) preparation of wall material is respectively that 110g/L, 150g/L, 190g/L colloid wall material are molten with distilled water compound concentration
Liquid.Wherein, every liter of 110g/L colloid wall material solution contain 25g octenyl succinic acid anhydrides modified gum arabic, 45g maltodextrins,
40g hydroxypropylβ-cyclodextrins, 0.10gEDTA-2Na；Every liter of 150g/L colloid wall material solution contains 25g octenyl succinic acid anhydrides and changed
Property Arabic gum, 65g maltodextrins, 60g hydroxypropylβ-cyclodextrins, 0.10gEDTA-2Na；Every liter of 190g/L colloid wall material solution
Contain 25g octenyl succinic acid anhydrides modified gum arabic, 85g maltodextrins, 80g hydroxypropylβ-cyclodextrins, 0.10gEDTA-
2Na；
(2) preparation of core is using 80% ethanol solution containing 1% citric acid as extractant, purple corn cob powder and extractant
Ratio be 1:8 (W/V), extracted 2 times at 55 DEG C, each extraction time is 60min, obtains thick purple corn cob anthocyanin solution
(a).Addition HP20 macroreticular resins in a, HP20 macroreticular resins and a ratio are 1:60 (W/V), it is placed in 25 DEG C of shaking baths
Filtered after 100r/min vibration absorption 5h, then the anthocyanin of the ethanol solution elution resin adsorption with 95%, elution time 8h,
Eluent is dissolved after ethanol is removed in decompression with distilled water, obtains purple corn cob anthocyanin solution (b) after purification.Anthocyanin in b
Content be 1.27g/100g (dry weight)；
(3) the gradient embedding of purple corn cob anthocyanin adds 110g/L colloid wall material solution in b solution while stirring,
Wall material is 0.75 with core mass ratio:1, after 60 DEG C of constant temperature stir 15min, add 150g/L colloid wall material solution, wall material
It is 0.75 with core mass ratio:1, after 60 DEG C of constant temperature stir 20min, homogeneous 1 time, adds 190g/ under 40MPa pressure conditions
L colloid wall material solution, wall material are 0.75 with core mass ratio:Homogeneous 1 time under Isosorbide-5-Nitrae 0MPa pressure conditions, after being embedded
Purple corn cob anthocyanin compound.Compared with 190g/L colloid wall material solution whole process isoconcentration embedding.
(4) dry and the purple corn cob anthocyanin compound after embedding, EAT 180 are dried using spray drying process
DEG C, leaving air temp is 85 DEG C, and compound concentration need not be controlled when drying.
Embodiment 3：
(1) preparation of wall material is respectively that 120g/L, 160g/L, 200g/L colloid wall material are molten with distilled water compound concentration
Liquid.Wherein, every liter of 120g/L colloid wall material solution contain 30g octenyl succinic acid anhydrides modified gum arabic, 45g maltodextrins,
45g hydroxypropylβ-cyclodextrins, 0.10gEDTA-2Na；Every liter of 160g/L colloid wall material solution contains 30g octenyl succinic acid anhydrides and changed
Property Arabic gum, 65g maltodextrins, 65g hydroxypropylβ-cyclodextrins, 0.10gEDTA-2Na；Every liter of 200g/L colloid wall material solution
Contain 30g octenyl succinic acid anhydrides modified gum arabic, 85g maltodextrins, 85g hydroxypropylβ-cyclodextrins, 0.10gEDTA-
2Na；
(2) preparation of core is using 80% ethanol solution containing 1% citric acid as extractant, purple corn cob powder and extractant
Ratio be 1:9 (W/V), extracted 2 times at 55 DEG C, each extraction time is 60min, obtains thick purple corn cob anthocyanin solution
(a).Addition HP20 macroreticular resins in a, HP20 macroreticular resins and a ratio are 1:50 (W/V), it is placed in 25 DEG C of shaking baths
Filtered after 100r/min vibration absorption 5h, then the anthocyanin of the ethanol solution elution resin adsorption with 95%, elution time 9h,
Eluent is dissolved after ethanol is removed in decompression with distilled water, obtains purple corn cob anthocyanin solution (b) after purification.Anthocyanin in b
Content be 1.46g/100g (dry weight)；
(3) the gradient embedding of purple corn cob anthocyanin adds 120g/L colloid wall material solution in b solution while stirring,
Wall material is 0.75 with core mass ratio:1, after 60 DEG C of constant temperature stir 15min, add 160g/L colloid wall material solution, wall material
It is 0.75 with core mass ratio:1, after 60 DEG C of constant temperature stir 20min, homogeneous 1 time, adds 200g/ under 40MPa pressure conditions
L colloid wall material solution, wall material are 0.75 with core mass ratio:Homogeneous 1 time under Isosorbide-5-Nitrae 0MPa pressure conditions, after being embedded
Purple corn cob anthocyanin compound.Compared with 200g/L colloid wall material solution whole process isoconcentration embedding.
(4) dry and the purple corn cob anthocyanin compound after embedding, EAT 180 are dried using spray drying process
DEG C, leaving air temp is 85 DEG C, and compound concentration need not be controlled when drying.
Embodiment 4：
(1) preparation of wall material is respectively that 130g/L, 170g/L, 210g/L colloid wall material are molten with distilled water compound concentration
Liquid.Wherein, every liter of 130g/L colloid wall material solution contain 35g octenyl succinic acid anhydrides modified gum arabic, 50g maltodextrins,
45g hydroxypropylβ-cyclodextrins, 0.15gEDTA-2Na；Every liter of 170g/L colloid wall material solution contains 35g octenyl succinic acid anhydrides and changed
Property Arabic gum, 70g maltodextrins, 65g hydroxypropylβ-cyclodextrins, 0.15gEDTA-2Na；Every liter of 210g/L colloid wall material solution
Contain 35g octenyl succinic acid anhydrides modified gum arabic, 90g maltodextrins, 85g hydroxypropylβ-cyclodextrins, 0.15gEDTA-
2Na；
(2) preparation of core is using 80% ethanol solution containing 1% citric acid as extractant, purple corn cob powder and extractant
Ratio be 1:10 (W/V), extracted 2 times at 55 DEG C, each extraction time is 60min, and it is molten to obtain thick purple corn cob anthocyanin
Liquid (a).Addition HP20 macroreticular resins in a, HP20 macroreticular resins and a ratio are 1:40 (W/V), it is placed in 25 DEG C of shaking baths
Filtered after 100r/min vibration absorption 5h, then the anthocyanin of the ethanol solution elution resin adsorption with 95%, elution time 9h,
Eluent is dissolved after ethanol is removed in decompression with distilled water, obtains purple corn cob anthocyanin solution (b) after purification.Anthocyanin in b
Content be 1.66g/100g (dry weight)；
(3) the gradient embedding of purple corn cob anthocyanin adds 130g/L colloid wall material solution in b solution while stirring,
Wall material is 1 with core mass ratio:1, after 60 DEG C of constant temperature stir 15min, add 170g/L colloid wall material solution, wall material and core
Material mass ratio is 1:1, after 60 DEG C of constant temperature stir 20min, homogeneous 1 time under 40MPa pressure conditions, add 210g/L colloid
Wall material solution, wall material are 1 with core mass ratio:Homogeneous 1 time under Isosorbide-5-Nitrae 0MPa pressure conditions, the purple corn cob flower after being embedded
Color glycosides compound.Compared with 210g/L colloid wall material solution whole process isoconcentration embedding.
(4) dry and the purple corn cob anthocyanin compound after embedding, EAT 180 are dried using spray drying process
DEG C, leaving air temp is 85 DEG C, and compound concentration need not be controlled when drying.
Embodiment 5：
(1) preparation of wall material is respectively that 140g/L, 180g/L, 220g/L colloid wall material are molten with distilled water compound concentration
Liquid.Wherein, every liter of 140g/L colloid wall material solution contain 40g octenyl succinic acid anhydrides modified gum arabic, 50g maltodextrins,
50g hydroxypropylβ-cyclodextrins, 0.2gEDTA-2Na；Every liter of 180g/L colloid wall material solution contains 40g octenyl succinic acid anhydrides and changed
Property Arabic gum, 70g maltodextrins, 70g hydroxypropylβ-cyclodextrins, 0.2gEDTA-2Na；Every liter of 220g/L colloid wall material solution
Contain 40g octenyl succinic acid anhydrides modified gum arabic, 90g maltodextrins, 90g hydroxypropylβ-cyclodextrins, 0.2gEDTA-2Na；
(2) preparation of core is using 80% ethanol solution containing 1% citric acid as extractant, purple corn cob powder and extractant
Ratio be 1:10 (W/V), extracted 2 times at 55 DEG C, each extraction time is 60min, and it is molten to obtain thick purple corn cob anthocyanin
Liquid (a).Addition HP20 macroreticular resins in a, HP20 macroreticular resins and a ratio are 1:40 (W/V), it is placed in 25 DEG C of shaking baths
Filtered after 100r/min vibration absorption 5h, then the anthocyanin of the ethanol solution elution resin adsorption with 95%, elution time are
10h, eluent are dissolved after ethanol is removed in decompression with distilled water, obtain purple corn cob anthocyanin solution (b) after purification.Flower in b
The content of color glycosides is 1.72g/100g (dry weight)；
(3) the gradient embedding of purple corn cob anthocyanin adds 140g/L colloid wall material solution in b solution while stirring,
Wall material is 1 with core mass ratio:1, after 60 DEG C of constant temperature stir 15min, add 180g/L colloid wall material solution, wall material and core
Material mass ratio is 1:1, after 60 DEG C of constant temperature stir 20min, homogeneous 1 time under 40MPa pressure conditions, add 220g/L colloid
Wall material solution, wall material are 1 with core mass ratio:Homogeneous 1 time under Isosorbide-5-Nitrae 0MPa pressure conditions, the purple corn cob flower after being embedded
Color glycosides compound.Compared with 220g/L colloid wall material solution whole process isoconcentration embedding.
(4) dry and the purple corn cob anthocyanin compound after embedding, EAT 180 are dried using spray drying process
DEG C, leaving air temp is 85 DEG C, and compound concentration need not be controlled when drying.
For those skilled in the art, it can still be carried out to the technical scheme described in foregoing embodiments
Modification, or equivalent substitution is carried out to which part technical characteristic, within the spirit and principles of the invention, that is made is any
Modification, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (1)
1. a kind of purple corn cob anthocyanin dynamic gradient embedding method, it is characterised in that comprise the following steps：
Step 1, the preparation of wall material, it is respectively 100-130g/L, 140-170g/L, 180-210g/L with distilled water compound concentration
Colloid wall material solution；Wherein, every liter of colloid wall material solution contains 20-40g octenyl succinic acid anhydrides modified gum arabic, 40-
100g maltodextrins, 40-100g hydroxypropylβ-cyclodextrins, 0.05-0.20gEDTA-2Na；
Step 2, the preparation of core, using 80% ethanol solution containing 1% citric acid as extractant, purple corn cob powder and extraction
The ratio of agent is 1:8-1:10W/V, extracted 2 times at 55 DEG C, each extraction time is 60min, obtains thick purple corn cob pattern
Glycosides solution a；HP20 macroreticular resins, HP20 macroreticular resins and thick purple corn cob pattern are added in thick purple corn cob anthocyanin solution a
Glycosides solution a ratio is 1:40-1:70W/V, filtered after being placed in 25 DEG C of shaking bath 100r/min vibration absorption 5h, then with 95%
Ethanol solution elution resin adsorption anthocyanin, elution time 6-10h, eluent through decompression remove ethanol after with distill it is water-soluble
Solution, obtains purple corn cob anthocyanin solution b after purification；The dry weight content 1.05- of anthocyanin in purple corn cob anthocyanin solution b
1.72g/100g；
Step 3, the gradient embedding of purple corn cob anthocyanin, purple is added by 100-130g/L colloid wall material solution while stirring
In corncob anthocyanin solution b, wall material and core mass ratio are 0.5-0.75:1, after 60 DEG C of constant temperature stir 15-20min, add
Enter 140-170g/L colloid wall material solution, wall material and core mass ratio are 0.75-1.0:1, stir 15- in 60 DEG C of constant temperature
After 20min, homogeneous 1 time under 40MPa pressure conditions, 180-210g/L colloid wall material solution, wall material and core quality are added
Than for 0.75-1.0:Homogeneous 1 time under Isosorbide-5-Nitrae 0MPa pressure conditions, the purple corn cob anthocyanin compound after being embedded；
Step 4, dry, the purple corn cob anthocyanin compound after embedding, EAT 180 are dried using spray drying process
DEG C, leaving air temp is 85 DEG C, and compound concentration need not be controlled when drying.
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| CN108576817B (en) * | 2018-03-23 | 2021-07-02 | 河南科技大学 | Preparation method of slow-release type anthocyanin microcapsules |
| CN108741071A (en) * | 2018-05-24 | 2018-11-06 | 中北大学 | A kind of preparation method of purple perilla anthocyanin microcapsules |
| CN111513146A (en) * | 2020-04-30 | 2020-08-11 | 江苏中兴药业有限公司 | Preparation method of silybum marianum oil containing probiotics |
| CN113150045A (en) * | 2021-04-16 | 2021-07-23 | 山西火品生物科技有限公司 | Method for efficiently extracting and purifying anthocyanin of purple corn cob |
| CN115152927B (en) * | 2022-06-01 | 2023-12-22 | 沈阳农业大学 | Purple corn anthocyanin compound and preparation method thereof |
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| CN102229753A (en) * | 2011-04-20 | 2011-11-02 | 淮北普豪生物科技有限公司 | Method for extracting purple corn pigment |
| CN102229755B (en) * | 2011-05-16 | 2013-11-27 | 武汉绿孚生物工程有限责任公司 | Method for producing purple sweet potato haematochrome |
| CN102334685A (en) * | 2011-05-27 | 2012-02-01 | 陕西科技大学 | Preparation method of buckwheat flavone microcapsule and its product |
| CN103070329A (en) * | 2011-10-25 | 2013-05-01 | 沈阳医学院 | Preparation method for microcapsule maize purple plant anthocyanin pigment |
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