CN105232593A - Nitraria branch and leaf extract - Google Patents
Nitraria branch and leaf extract Download PDFInfo
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- CN105232593A CN105232593A CN201510711944.4A CN201510711944A CN105232593A CN 105232593 A CN105232593 A CN 105232593A CN 201510711944 A CN201510711944 A CN 201510711944A CN 105232593 A CN105232593 A CN 105232593A
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Abstract
The invention relates to a nitraria branch and leaf extract. Nitraria branches and leaves obtained through cleaning, drying, crushing, bur picking, extraction, purification and the like have the health-care functions of delaying aging and reducing blood glucose and blood fat. A preparation method of the nitraria branch and leaf general flavone extract is simple in process, easy to operate, relatively high in yield and favorable in quality and facilitates industrial production and processing.
Description
Technical field
The present invention relates to plant extract field, be specially a kind of extract and preparation method thereof with slow down aging, blood sugar lowering, antilipemic healthy function Cortex Acanthopanacis Radicis branch and leaf.
Background technology
Cortex Acanthopanacis Radicis is the plant of zygophyllaceae Nitraria, for machaka, Baici plant relies on the raw physiological function of various forms of drought and xeromorphy, adapt to environment facies in a variety of forms, multiply tenaciously, survive and form vegetational type, prevent drift sand from invading oasis, keep the stable of oasis environment to have very large effect, therefore, Cortex Acanthopanacis Radicis is the excellent reproducting tree species of ideal administering northwestern desert and salt-soda soil, greening environment, improvement soil, local resident's living standard of improving the ecological environment, improve.And can as the green tree species in conceding the land to forestry, saline and alkaline section.
Cortex Acanthopanacis Radicis is the endemic species of China, contains abundant nutritional labeling and active substance, can be used as medicine, have regulating menstruation and activating blood except in its fruit, and outside the effects such as digestion-promoting spleen-invigorating, we find in the branch and leaf of Cortex Acanthopanacis Radicis also containing abundant flavones ingredient.Cortex Acanthopanacis Radicis branch and leaf are mainly used as the forage grass of domestic animal for a long time, are not further developed.But we find that flavones ingredient wherein has certain oxidation resistance, possess the effects such as Adjust-blood lipid antioxidation, blood pressure lowering and blood sugar lowering equally.
Currently available technology is not still to the research that Cortex Acanthopanacis Radicis branch and leaf extractive of general flavone extracts, and be only that simply to adopt ethanol to carry out the not high flavone yield that causes of extracting method efficiency lower, organic solvent reclaims not exclusively, and extraction cost is higher etc.
Summary of the invention
The object of this invention is to provide the extracting method of the Cortex Acanthopanacis Radicis branch and leaf total flavones that a kind of loss of effective components is few, quality is high, extraction cost is low.The Cortex Acanthopanacis Radicis branch and leaf total flavones that the method extracts possesses the effects such as Adjust-blood lipid, antioxidation, blood pressure lowering, slow down aging and blood sugar lowering.
Concrete, extracting method of the present invention is as follows:
An extracting method for Cortex Acanthopanacis Radicis branch and leaf extract, is characterized in that, adopts Cortex Acanthopanacis Radicis branch and leaf, after fragmentation, adopts 70% alcohol reflux.
An extracting method for Cortex Acanthopanacis Radicis branch and leaf extract, is characterized in that comprising the following steps:
1, clear up: select ripe Cortex Acanthopanacis Radicis branch and leaf, screen out silt, clean with clear water;
2, dry: by the Cortex Acanthopanacis Radicis branch and leaf cold drying 10 ~ 24h at 5 ~ 30 DEG C cleaned up;
3, broken: Cortex Acanthopanacis Radicis branch and leaf step 2 obtained carry out micronizing, and obtaining particle size range is 1 ~ 75 μm of powder;
4, extract: 50% ethanol ~ 80% ethanol adding 5 ~ 8 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3 obtained carries out supersound extraction 0.5h ~ 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 50% ethanol ~ 80% ethanol filtering residue I being reused 5 ~ 8 times amount carries out reflux, extract, 0.5h ~ 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the total flavones crude extract part of Cortex Acanthopanacis Radicis branch and leaf;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the different in flow rate loading of 0.2 ~ 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.5 ~ 1.5ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
This extracting method be through a large amount of experiment of inventor determine and screen obtain, can preparation process be reduced, and the Cortex Acanthopanacis Radicis branch and leaf extractive of general flavone that loss of effective components is few, quality is high, cost is low can be obtained.
In the first step, if carefully do not clean, the silt dead leaf etc. of doping can affect extraction efficiency, even causes mechanical breakdown and accident.
In second step, under low temperature, carry out drying, the loss of flavone effective ingredient can be ensured.
In 3rd step, superfine communication technique can make powder diameter little (generally below 15 μm) and be evenly distributed, ball degree and homogeneity obviously improve, bulk density and specific surface significantly improve, plant cell wall breaking rate is high, dissolving and the release of the effective ingredient (particularly refractory components) in Cortex Acanthopanacis Radicis branch and leaf are accelerated, and the dissolution rate of effective ingredient is accelerated.
In 4th step, have employed two kinds of extracting modes, first supersound extraction is carried out, hyperacoustic sound wave cavitation makes solid sample disperse, increase the contact area between sample and extractant, improve object transfers to liquid phase mass transfer speed from solid phase, period also carries out stirring with agitator and can reduce powder, adds the contact area of medical material and solvent.Next adopts reflux, extract, and extract solid-liquid ratio used by optimizing, extraction time, Extracting temperature and concentration of alcohol can increase substantially the general flavone content of Cortex Acanthopanacis Radicis branch and leaf.Said extracted process only has two steps, simplifies the process of preparation, can not only substantially increase the efficiency of extraction step, and can reduce the loss of preparation process.
In 5th step, the separation and purification of polyamide column chromatography can obtain purer flavone extract.
In addition, for the consideration of controlling cost, the cold drying in each step of the present invention, backflow, ultrasonic, etc. are all ripe technology, simple to operate, easy left-hand seat.But compared with prior art, simplify leaching process and but obtain higher yield and the good product of quality.
Preferably, a kind of extracting method of Cortex Acanthopanacis Radicis branch and leaf extract, is characterized in that comprising the following steps:
1, clear up: select ripe Cortex Acanthopanacis Radicis branch and leaf, screen out silt, clean with clear water;
2, dry: by the Cortex Acanthopanacis Radicis branch and leaf cold drying 15h at 15 DEG C cleaned up;
3, broken: Cortex Acanthopanacis Radicis branch and leaf step 2 obtained carry out micronizing, obtain Cortex Acanthopanacis Radicis branch and leaf powder;
4, extract: 50% ethanol adding 5 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3 obtained carries out supersound extraction 0.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 60% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.3ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.5ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
Preferably, a kind of extracting method of Cortex Acanthopanacis Radicis branch and leaf extract, is characterized in that comprising the following steps:
1, clear up: select ripe Cortex Acanthopanacis Radicis branch and leaf, screen out silt, clean with clear water;
2, dry: by the Cortex Acanthopanacis Radicis branch and leaf cold drying 20h at 20 DEG C cleaned up;
3, broken: Cortex Acanthopanacis Radicis branch and leaf step 2 obtained carry out micronizing, obtain Cortex Acanthopanacis Radicis branch and leaf powder;
4, extract: 50% ethanol adding 8 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3 obtained carries out supersound extraction 0.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 60% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf.
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.5ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.0ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
Preferably, a kind of extracting method of Cortex Acanthopanacis Radicis branch and leaf extract, is characterized in that comprising the following steps:
1, clear up: select ripe Cortex Acanthopanacis Radicis branch and leaf, screen out silt, clean with clear water;
2, dry: by the Cortex Acanthopanacis Radicis branch and leaf cold drying 15h at 15 DEG C cleaned up;
3, broken: micronizing to be carried out to Cortex Acanthopanacis Radicis branch and leaf, obtains Cortex Acanthopanacis Radicis branch and leaf powder;
4, extract: 50% ethanol adding 5 times amount in Cortex Acanthopanacis Radicis branch and leaf powder is carried out supersound extraction 0.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 60% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
Detailed description of the invention
Embodiment 1
1, clear up: select ripe Cortex Acanthopanacis Radicis branch and leaf 10kg, screen out silt, clean with clear water;
2, dry: by the Cortex Acanthopanacis Radicis branch and leaf cold drying 15h at 15 DEG C cleaned up;
3, broken: Cortex Acanthopanacis Radicis branch and leaf step 2 obtained carry out micronizing, obtain Cortex Acanthopanacis Radicis branch and leaf powder;
4, extract: 70% ethanol adding 5 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3 obtained carries out supersound extraction 1h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.0ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
After testing, the extraction ratio of the Cortex Acanthopanacis Radicis branch and leaf total flavones that embodiment 1 is extracted is 6.235% far above about 4% of prior art, and therefore the present invention has higher beneficial effect for the extraction of Cortex Acanthopanacis Radicis branch and leaf total flavones.
Embodiment 2
1, clear up: select ripe Cortex Acanthopanacis Radicis branch and leaf 10kg, screen out silt, clean with clear water;
2, dry: by the Cortex Acanthopanacis Radicis branch and leaf cold drying 15h at 20 DEG C cleaned up;
3, broken: Cortex Acanthopanacis Radicis branch and leaf step 2 obtained carry out micronizing, obtain Cortex Acanthopanacis Radicis branch and leaf powder;
4, extract: 70% ethanol adding 8 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3 obtained carries out supersound extraction 1h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 80% ethanol filtering residue I being reused 6 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.7ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.5ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
After testing, the extraction ratio of the Cortex Acanthopanacis Radicis branch and leaf total flavones that embodiment 2 is extracted is 5.235% far above about 4% of prior art, and therefore the present invention has higher beneficial effect for the extraction of Cortex Acanthopanacis Radicis branch and leaf total flavones.
Embodiment 3
1, clear up: select ripe Cortex Acanthopanacis Radicis branch and leaf 10kg, screen out silt, clean with clear water;
2, dry: by the Cortex Acanthopanacis Radicis branch and leaf cold drying 20h at 15 DEG C cleaned up;
3, broken: micronizing to be carried out to the Cortex Acanthopanacis Radicis branch and leaf that step 2 obtains, obtains Cortex Acanthopanacis Radicis branch and leaf powder;
4, extract: 70% ethanol adding 5 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3 obtained carries out supersound extraction 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 65% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5, purification: will through pretreated Buddhist nun's human relations polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.6ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.8ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
After testing, the extraction ratio of the Cortex Acanthopanacis Radicis branch and leaf total flavones that embodiment 3 is extracted is 6.216% far above about 4% of prior art, and therefore the present invention has higher beneficial effect for the extraction of Cortex Acanthopanacis Radicis branch and leaf total flavones.
Embodiment 4
The extraction effect contrast of the total flavones of dissimilar chromatogram purification
1, the preparation of standard solution
Precision takes control substance of Rutin 0.025g, dissolves with absolute methanol, and being settled to 100ml, to be made into concentration be 250mg/l, and draw the dilution of this solution 10.0ml absolute methanol and be settled to 100ml, being made into concentration is 25mg/l.
2, the drafting of standard curve and sample tests
The rutin contrast solution 0.00 of accurate absorption 25mg/l, 1.00,2.00,3.00,4.00,5.00,6.00ml is placed in 10ml color comparison tube absolute methanol and is settled to scale, in the uv absorption of 357nm place bioassay standard solution and sample, obtain standard curve regression equation.General flavone content in sample is gone out by regression equation calculation.
3, preparation to be measured
Extractive of general flavone to be measured all adopts embodiment 1 method to prepare except chromatographic column type difference.
4, experimental result
As shown in table 1, the polyamide type that the present invention adopts can improve the extraction efficiency of total flavones effectively
The dissimilar chromatographic column of table 1 is to the content balance of purification total flavones
Chromatographic column type | Silica gel | Sephadax-G | Sephadax-LH20 | Polyamide |
Absorbance | 0.591 | 0.523 | 0.632 | 0.921 |
Account for raw medicinal herbs content (%) | 4.493 | 3.912 | 4.751 | 6.235 |
The extraction effect contrast of the total flavones that embodiment 5 Different Extraction Method obtains
1, the preparation of standard solution
Precision takes control substance of Rutin 0.025g, and dissolve with absolute methanol, be settled to 100ml, being made into concentration is 250mg/l, draws this solution 10.0ml, and be settled to 100ml with absolute methanol dilution, being made into concentration is 25mg/l.
The drafting of 2 standard curves and sample tests
The rutin contrast solution 0.00 of accurate absorption 25mg/l, 1.00,2.00,3.00,4.00,5.00,6.00ml is placed in 10ml color comparison tube absolute methanol and is settled to scale, in the uv absorption of 357nm place bioassay standard solution and sample, obtain standard curve regression equation.General flavone content in sample is gone out by regression equation calculation.
The preparation of 3 products to be tested
Extractive of general flavone to be measured all adopts embodiment 1 method to prepare except extracting method difference.
4 experimental results
As shown in table 2, the total flavones that the present invention prepares is far above the extraction effect of prior art.
The contrast of the general flavone content that table 2 Different Extraction Method obtains
The foregoing is only preferred embodiment of the present invention, and be not used to limit substantial technological context of the present invention, substantial technological content of the present invention is broadly defined in the right of application, any technology entities that other people complete or method, if with application right define identical, also or a kind of change of equivalence, be all covered by being regarded as among this right.
Claims (6)
1. an extracting method for Cortex Acanthopanacis Radicis branch and leaf extract, is characterized in that, adopts Cortex Acanthopanacis Radicis branch and leaf, after fragmentation, adopts 70% alcohol reflux.
2. an extracting method for Cortex Acanthopanacis Radicis branch and leaf extract, is characterized in that comprising the following steps:
1) select ripe Cortex Acanthopanacis Radicis branch and leaf, screen out silt, clean with clear water;
2) the Cortex Acanthopanacis Radicis branch and leaf cold drying 10 ~ 24h at 5 ~ 30 DEG C will cleaned up;
3) to step 2) the Cortex Acanthopanacis Radicis branch and leaf that obtain carry out micronizing, and obtaining particle size range is 1 ~ 75 μm of powder;
4) 50% ~ 80% ethanol adding 5 ~ 8 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3) obtained carries out supersound extraction 0.5h ~ 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 50% ethanol ~ 80% ethanol filtering residue I being reused 5 ~ 8 times amount carries out reflux, extract, 0.5h ~ 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the total flavones crude extract part of Cortex Acanthopanacis Radicis branch and leaf;
5) will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the different in flow rate loading of 0.2 ~ 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.5 ~ 1.5ml/min, eluent is merged, concentrated, be drying to obtain described Cortex Acanthopanacis Radicis branch and leaf extractive of general flavone.
3. preparation method according to claim 2, its feature, the preparation method of described Cortex Acanthopanacis Radicis branch and leaf extract is:
1) select ripe Cortex Acanthopanacis Radicis branch and leaf 10kg, screen out silt, clean with clear water;
2) the Cortex Acanthopanacis Radicis branch and leaf cold drying 15h at 15 DEG C will cleaned up;
3) by step 2) the Cortex Acanthopanacis Radicis branch and leaf that obtain carry out micronizing, obtain Cortex Acanthopanacis Radicis branch and leaf powder;
4) 70% ethanol adding 5 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3) obtained carries out supersound extraction 1h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5) through pretreated polyamide wet method dress post, above-mentioned crude extract will be got in the flow velocity loading of 0.8ml/min, and with the ethanol of 70% concentration with the different in flow rate eluting of 1.0ml/min, eluent be merged, concentrates, be drying to obtain the extractive of general flavone that purity is higher.
4. preparation method according to claim 2, its feature, the preparation method of described Cortex Acanthopanacis Radicis branch and leaf extract is:
1) select ripe Cortex Acanthopanacis Radicis branch and leaf 10kg, screen out silt, clean with clear water;
2) the Cortex Acanthopanacis Radicis branch and leaf cold drying 14h at 16 DEG C will cleaned up;
3) by step 2) the Cortex Acanthopanacis Radicis branch and leaf that obtain carry out micronizing, obtain Cortex Acanthopanacis Radicis branch and leaf powder;
4) 70% ethanol adding 6 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3) obtained carries out supersound extraction 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 8 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5) through pretreated polyamide wet method dress post, above-mentioned crude extract will be got in the flow velocity loading of 0.75ml/min, and with the ethanol of 70% concentration with the different in flow rate eluting of 0.9ml/min, eluent be merged, concentrates, be drying to obtain the flavone extract that purity is higher.
5. preparation method according to claim 2, its feature, the preparation method of described Cortex Acanthopanacis Radicis branch and leaf extract is:
1) select ripe Cortex Acanthopanacis Radicis branch and leaf 10kg, screen out silt, clean with clear water;
2) the Cortex Acanthopanacis Radicis branch and leaf cold drying 13h at 17 DEG C will cleaned up;
3) to step 2) the Cortex Acanthopanacis Radicis branch and leaf that obtain carry out micronizing, obtain Cortex Acanthopanacis Radicis branch and leaf powder;
4) 70% ethanol adding 7 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3) obtained carries out supersound extraction 1.2h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1.1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Cortex Acanthopanacis Radicis branch and leaf;
5) will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.85ml/min, eluent is merged, concentrated, be drying to obtain the extractive of general flavone that purity is higher.
6. have a Cortex Acanthopanacis Radicis branch and leaf extract for slow down aging, blood sugar lowering, antilipemic healthy function, its special type is, described Cortex Acanthopanacis Radicis branch and leaf method for preparing extractive comprises the following steps:
1) select ripe Cortex Acanthopanacis Radicis branch and leaf, screen out silt, clean with clear water;
2) the Cortex Acanthopanacis Radicis branch and leaf cold drying 10 ~ 24h at 5 ~ 30 DEG C will cleaned up;
3) to step 2) the Cortex Acanthopanacis Radicis branch and leaf that obtain carry out micronizing, and obtaining particle size range is 1 ~ 75 μm of powder;
4) 50% ethanol ~ 80% ethanol adding 5 ~ 8 times amount in Cortex Acanthopanacis Radicis branch and leaf powder step 3) obtained carries out supersound extraction 0.5h ~ 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 50% ethanol ~ 80% ethanol filtering residue I being reused 5 ~ 8 times amount carries out reflux, extract, 0.5h ~ 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains Cortex Acanthopanacis Radicis branch and leaf crude extract part;
5) will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the different in flow rate loading of 0.2 ~ 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.5 ~ 1.5ml/min, eluent is merged, concentrated, be drying to obtain the Cortex Acanthopanacis Radicis branch and leaf extract that purity is higher.
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