CN105225590A - For setting up source of the gas device for drawing-in and pumping-out and its implementation of respiratory failure animal model - Google Patents

Abstract

The invention provides a kind of source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model and its implementation, described source of the gas device for drawing-in and pumping-out comprises air-source air feeding device, pressure adjustment assembly, mixing valve, accumulator unit, gas flow control device and lung ventilator, described air-source air feeding device is by oxygen gas tank, nitrogen gas tank and carbon dioxide canister composition, these three gas tanks are connected with respective pressure adjustment assembly with corresponding respectively, three pressure adjustment assemblys respectively with same mixing valve pipeline connection, described mixing valve connects the air intake opening of accumulator unit, the gas outlet of described accumulator unit and gas flow control device pipeline connection, described gas flow control device is connected with lung ventilator simultaneously, by making oxygen, nitrogen and carbon dioxide three kinds of gases with any melting concn specific output, set up I type and/or respiratory failure type Ⅱ animal model for respiration test.

Description

For setting up source of the gas device for drawing-in and pumping-out and its implementation of respiratory failure animal model

Technical field

The present invention relates to a kind of experimental provision and its implementation, especially a kind of source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model and its implementation.

Background technology

Present stage, the method causing animal used as test formation respiratory insufficiency is more, mostly adopts the method copying acute lung injury model at present both at home and abroad.It is generally acknowledged and cause the hazards of ALI to be divided into direct injury of lungs factor (i.e. lung inner mold injury of lungs) and indirect injury of lungs factor (i.e. lung external form injury of lungs).Lung inner mold injury of lungs experiment modeling method comprises physiological saline alveolar wass type, hydrochloric acid induction type, mechanical ventilation associated lung injury, oleic acid-induced and smoke inhalation type etc.The respiratory failure degree that such experimental technique brings out is difficult to control, and respiratory failure state poor repeatability.

Therefore, based on the demand of the progress of current respiratory failure animal model, research new device sets up comparatively stable, the animal model of respiratory failure state accurately for animal used as test, has very important using value.

Summary of the invention

Technical matters to be solved by this invention is to provide a kind of source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model.

Another technical matters to be solved by this invention is the implementation method providing the above-mentioned source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model.

For solving the problems of the technologies described above, technical scheme of the present invention is:

A kind of source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, comprise air-source air feeding device, pressure adjustment assembly, mixing valve, accumulator unit, gas flow control device and lung ventilator, described air-source air feeding device is by oxygen gas tank, nitrogen gas tank and carbon dioxide canister composition, these three gas tanks are connected with respective pressure adjustment assembly with corresponding respectively, three pressure adjustment assemblys respectively with same mixing valve pipeline connection, described mixing valve connects the air intake opening of accumulator unit, the gas outlet of described accumulator unit and gas flow control device pipeline connection, described gas flow control device is connected with lung ventilator simultaneously.

Preferably, the above-mentioned source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, described mixing valve and accumulator unit adopt mixing gas tank to realize, described three pressure adjustment assemblys directly with the air intake opening pipeline connection mixing gas tank.

Preferably, the above-mentioned source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, three air intake openings of described mixing gas tank are contour and each air intake opening is 30 ° of angles settings.

The implementation method of the above-mentioned source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, by making oxygen, nitrogen and carbon dioxide three kinds of gases with any melting concn specific output, set up I type and/or respiratory failure type Ⅱ animal model for respiration test, concrete grammar is the control by regulating the pressure of three kinds of gases to realize gas mixture concentration ratio, and wherein pressure regulating method Consideration is as follows:

The motion of gas in pipeline can be considered and establish steady flow at ambient temperature, then

Q _i＝V _iS _i(1)

Q in formula _ifor the volumetric flow rate of gas, V _ifor gas flow rate, S _ifor the cross-sectional area of airflow line, i=1,2,3, respectively corresponding oxygen, nitrogen and carbon dioxide three kinds of gases;

Consider the glutinousness of gas, utilize the Poiseuille law of fluid

$Q_i=\frac{{\mathrm{\πR}}_i^4\mathrm{\Δ}Pi}{8{\mathrm{\η}}_i{L}_i}---\left(2\right)$

R in formula _ifor the radius of airflow line, L _ifor the length ， ⊿ P of airflow line _ifor pressure difference before and after gas and vapor permeation, η _ifor the coefficient of viscosity of gas.Get R _i, L _ibe constant, then the flow of gas is only the function of the coefficient of viscosity and pressure difference, namely

Q _i＝f(ΔP _i,η _i)(3)

In temperature one timing, η _ifor experimental constant, only need Tiao Jie ⊿ P _ijust the flow control of this gas can be realized;

Owing to being steady flow, the concentration ratio of certain gas is decided by the ratio of its volumetric flow rate, and therefore the volumetric concentration ratio of certain gas is

$w_i=\frac{Q_i}{\underset{i=1}{\overset{3}{\Σ}}{Q}_i}\×100\%---\left(4\right)$

(2) formula and (3) formula are brought into (4) formula

$w_i=\frac{{\mathrm{\ΔP}}_i/{\mathrm{\η}}_i}{\underset{i=1}{\overset{3}{\Σ}}{\mathrm{\ΔP}}_i/{\mathrm{\η}}_i}\×100\%---\left(5\right)$

Just the concentration of certain gas can be obtained by (5) formula.

Preferably, the implementation method of the above-mentioned source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, considers at ambient temperature, and the coefficient of viscosity difference of three kinds of gases is little, and in test, gas flows among a small circle, can think η _ifor constant, (5) formula can be reduced to

$w_i=\frac{{\mathrm{\ΔP}}_i}{\underset{i=1}{\overset{3}{\Σ}}{\mathrm{\ΔP}}_i}\×100\%---\left(6\right).$

Structure of the present invention has following beneficial effect:

The above-mentioned source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, comparatively can stablize, set up respiratory failure state animal model accurately, by the composition of suction gas, the adjustment of proportioning, progressively reduces O in inhaling air ₂concentration (FiO ₂) and/or improve the CO of inhaling air ₂concentration (FiCO ₂), and be stabilized in the scope of a certain setting, work as PaO ₂<60mmHg and/or PaCO ₂during >50mmHg, namely form the acute respiratory failure state of animal used as test.

Accompanying drawing explanation

Fig. 1 is the structured flowchart of the source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model of the present invention.

Embodiment

For further illustrating the present invention, accompanying drawing is now coordinated to be described in detail:

Embodiment 1

As shown in Figure 1, the described source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, comprise air-source air feeding device, pressure adjustment assembly, mixing valve, accumulator unit, gas flow control device and lung ventilator, described air-source air feeding device is by oxygen gas tank, nitrogen gas tank and carbon dioxide canister composition, these three gas tanks are connected with respective pressure adjustment assembly with corresponding respectively, three pressure adjustment assemblys respectively with same mixing valve pipeline connection, described mixing valve connects the air intake opening of accumulator unit, the gas outlet of described accumulator unit and gas flow control device pipeline connection, described gas flow control device is connected with lung ventilator simultaneously.

Embodiment 2

The described source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model, comprise air-source air feeding device, pressure adjustment assembly, mixing gas tank, gas flow control device and lung ventilator, described air-source air feeding device is by oxygen gas tank, nitrogen gas tank and carbon dioxide canister composition, these three gas tanks are connected with respective pressure adjustment assembly with corresponding respectively, three pressure adjustment assemblys respectively with three the air intake opening pipeline connections mixing gas tank, these three air intake openings are contour and each air intake opening is 30 ° of angles settings, the gas outlet of described mixing gas tank and gas flow control device pipeline connection, described gas flow control device is connected with lung ventilator simultaneously.

Application examples

On embody rule, the using method of the above-mentioned source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model is: by making oxygen, nitrogen and carbon dioxide three kinds of gases with any melting concn specific output, set up I type and/or respiratory failure type Ⅱ animal model for respiration test, concrete grammar is the control by regulating the pressure of three kinds of gases to realize gas mixture concentration ratio.Specific experiment method for building animal model is as follows:

1, animal used as test: healthy adult male hybrid dog 9, body weight 25 ~ 35kg (experimental animal feeding station, Jinnan District, Tianjin provides).

2, analyze and calculate:

Three kinds of gas properties are stablized, and chemical reaction can not occur under normal temperature condition.For call for Votes is convenient, if three kinds of gas contents are 100%.Because test is carried out at ambient temperature, pressure is not too high, and the motion of gas in pipeline can be considered establishes steady flow, then have

Q _i＝V _iS _i(1)

Q in formula _ifor the volumetric flow rate of gas, V _ifor gas flow rate, S _ifor the cross-sectional area of airflow line, i=1,2,3, respectively corresponding oxygen, nitrogen and carbon dioxide three kinds of gases.

Consider the glutinousness of gas, have the Poiseuille law of fluid to know

$Q_i=\frac{{\mathrm{\&pi;R}}_i^4\mathrm{\&Delta;}Pi}{8{\mathrm{\&eta;}}_i{L}_i}---\left(2\right)$

R in formula _ifor the radius of airflow line, L _ifor the length ， ⊿ P of airflow line _ifor pressure difference before and after gas and vapor permeation, η _ifor the coefficient of viscosity of gas.Get R _i, L _ibe constant, then the flow of gas is only the function of the coefficient of viscosity and pressure difference, namely

Q _i＝f(ΔP _i,η _i)(3)

In temperature one timing, η _ifor experimental constant, therefore only need Tiao Jie ⊿ P _ijust the flow control of this gas can be realized.

Owing to being steady flow, the concentration ratio of certain gas is decided by the ratio of its volumetric flow rate, and therefore the volumetric concentration ratio of certain gas is

$w_i=\frac{Q_i}{\underset{i=1}{\overset{3}{\&Sigma;}}{Q}_i}\&times;100\%---\left(4\right)$

(2) formula and (3) formula are brought into (4) formula

$w_i=\frac{{\mathrm{\&Delta;P}}_i/{\mathrm{\&eta;}}_i}{\underset{i=1}{\overset{3}{\&Sigma;}}{\mathrm{\&Delta;P}}_i/{\mathrm{\&eta;}}_i}\&times;100\%---\left(5\right)$

Just the concentration of certain gas can be obtained by (5) formula.

Consider at ambient temperature, the coefficient of viscosity difference of three kinds of gases is little, and in test, gas flows among a small circle, can think η _ifor constant, (5) formula can be reduced to

$w_i=\frac{{\mathrm{\&Delta;P}}_i}{\underset{i=1}{\overset{3}{\&Sigma;}}{\mathrm{\&Delta;P}}_i}\&times;100\%---\left(6\right).$

From above formula, by regulating the pressure of three kinds of gases, the control of gas mixture concentration ratio just can be realized.

3, experimental technique

After animal used as test enters the room, weigh, Baoding, after foreleg vein administration general anesthesia, per os insert lengthen (I.D.) 9.0 ^#tracheal catheter, successively connects and exhales last carbon dioxide probe, breathing circuit, row mechanical ventilation.Mechanical ventilation condition: lung ventilator gas source interface and adjustable source of the gas device for drawing-in and pumping-out port of supplying gas is connected, tidal volume: 8-10ml/kg, frequency: 15 times/min, inspiratory/expiratory=1:1.5, end-tidal pressure: 2cmH ₂o.Maintain in experiment and anaesthetize and block autonomous respiration.100% air continues more than 20min, suitably regulates ventilation to arrange, with animal used as test without hypoxemia and CO according to arterial blood gas ₂state based on retention, in 2-4 hour, successively reduces FiO ₂, improve FiCO ₂, monitoring arterial blood gas, progressively makes experimental dog body arterial blood PaO ₂reach≤50mmHg, PaCO ₂reach>=70mmHg state after (i.e. respiratory failure state, hypoxemia and hypercapnia), maintain and to supply gas settings, in 1 hour, 2 hours, gather arterial blood, carry out blood gas analysis, record parameters of supplying gas, carry out data analysis.

4, statistical method

Measurement data is with mean ± standard deviation represent, application SPSS11.0 statistical package, carry out F inspection to comparing between each group of data group, result has statistical significance with P<0.05.

5, experimental result: experimental dog is basic, modeling 1h, 2h vim and vigour result is as shown in table 1.

PaO during experimental dog base state ₂110.64 ± 8.56mmHg, PaCO ₂39.67 ± 3.38mmHg.During modeling 1h, blood gas analysis indices all comparatively base state has remarkable change, P value < 0.01, PaO ₂46.17 ± 3.03mmHg.

Table 1 respiratory failure animal model arterial blood gas analysis statistics

Note: in table 1 show that the blood gas index of this state compares with base state, its P value < 0.01; show that more other two groups of this state group compares the equal < 0.01 of its P value

The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (5)

1. one kind for setting up the source of the gas device for drawing-in and pumping-out of respiratory failure animal model, it is characterized in that: comprise air-source air feeding device, pressure adjustment assembly, mixing valve, accumulator unit, gas flow control device and lung ventilator, described air-source air feeding device is by oxygen gas tank, nitrogen gas tank and carbon dioxide canister composition, these three gas tanks are connected with respective pressure adjustment assembly with corresponding respectively, three pressure adjustment assemblys respectively with same mixing valve pipeline connection, described mixing valve connects the air intake opening of accumulator unit, the gas outlet of described accumulator unit and gas flow control device pipeline connection, described gas flow control device is connected with lung ventilator simultaneously.

2. the source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model according to claim 1, it is characterized in that: described mixing valve and accumulator unit adopt mixing gas tank to realize, described three pressure adjustment assemblys directly with the air intake opening pipeline connection mixing gas tank.

3. the source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model according to claim 2, is characterized in that: three air intake openings of described mixing gas tank are contour and each air intake opening is 30 ° of angles settings.

4. the implementation method of the source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model according to claim 1, it is characterized in that: by making oxygen, nitrogen and carbon dioxide three kinds of gases with any melting concn specific output, set up I type and/or respiratory failure type Ⅱ animal model for respiration test, concrete grammar is the control by regulating the pressure of three kinds of gases to realize gas mixture concentration ratio, and wherein pressure regulating method Consideration is as follows:

The motion of gas in pipeline can be considered and establish steady flow at ambient temperature, then

Q _i＝V _iS _i(1)

Q in formula _ifor the volumetric flow rate of gas, V _ifor gas flow rate, S _ifor the cross-sectional area of airflow line, i=1,2,3, respectively corresponding oxygen, nitrogen and carbon dioxide three kinds of gases;

Consider the glutinousness of gas, utilize the Poiseuille law of fluid

$Q_i=\frac{{\mathrm{\&pi;R}}_i^4{\mathrm{\&Delta;P}}_i}{8{\mathrm{\&eta;}}_i{L}_i}---\left(2\right)$

R in formula _ifor the radius of airflow line, L _ifor the length ， ⊿ P of airflow line _ifor pressure difference before and after gas and vapor permeation, η _ifor the coefficient of viscosity of gas.Get R _i, L _ibe constant, then the flow of gas is only the function of the coefficient of viscosity and pressure difference, namely

Q _i＝f(ΔP _i,η _i)(3)

In temperature one timing, η _ifor experimental constant, only need Tiao Jie ⊿ P _ijust the flow control of this gas can be realized;

Owing to being steady flow, the concentration ratio of certain gas is decided by the ratio of its volumetric flow rate, and therefore the volumetric concentration ratio of certain gas is

$w_i=\frac{Q_i}{\underset{i=1}{\overset{3}{\&Sigma;}}{Q}_i}\&times;100\%---\left(4\right)$

(2) formula and (3) formula are brought into (4) formula

$w_i=\frac{{\mathrm{\&Delta;P}}_i/{\mathrm{\&eta;}}_i}{\underset{i=1}{\overset{3}{\&Sigma;}}{\mathrm{\&Delta;P}}_i/{\mathrm{\&eta;}}_i}\&times;100\%---\left(5\right)$

Just the concentration of certain gas can be obtained by (5) formula.

5. the implementation method of the source of the gas device for drawing-in and pumping-out for setting up respiratory failure animal model according to claim 4, described (5) formula can be reduced to

$w_i=\frac{{\mathrm{\&Delta;P}}_i}{\underset{i=1}{\overset{3}{\&Sigma;}}{\mathrm{\&Delta;P}}_i}\&times;100\%---\left(6\right).$