CN105223371A - A kind of bench top flow cytometer sheath fluid device for casting - Google Patents
A kind of bench top flow cytometer sheath fluid device for casting Download PDFInfo
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- CN105223371A CN105223371A CN201410331550.1A CN201410331550A CN105223371A CN 105223371 A CN105223371 A CN 105223371A CN 201410331550 A CN201410331550 A CN 201410331550A CN 105223371 A CN105223371 A CN 105223371A
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- flow cytometer
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Abstract
A kind of bench top flow cytometer sheath fluid device for casting, is made up of pipeline, rear pump, flow set, flow detection, flow indication and driver part.Wherein, described rear pump is by pipeline sticking up flow container and being interconnected with the liquid chamber that sticks up of flow cytometer, and described flow set, driver part, rear pump, flow detection and flow indication are electrically connected mutually.A kind of bench top flow cytometer sheath fluid device for casting of the present invention, after being attached to the use of bench top flow cytometer, sheath fluid is delivered directly to the sheath liquid chamber of flow cytometer from sealing state, solve the pollution problem of the bench top flow cytometer sheath perfusion of present stage a large amount of import, avoid the infringement that surrounding environment causes bench top flow cytometer, improve the accuracy of measurement result, and simple to operate, improve automation mechanized operation degree.
Description
Technical field
The invention belongs to medical instruments field, particularly relate to a kind of device of bench top flow cytometer sheath perfusion.
Background technology
Flow cytometer (FCM) has become contemporary analytical cytology field and has detected one of main tool of cellular elements feature, is used widely in the subjects such as clinical medicine, biology and oncology.Flow cytometry is also known as Fluorescence-activated cell sorting, and the screening of cell realizes containing single celled liquid stream by being separated, and this liquid stream is exactly sheath fluid.The effect one of sheath fluid is by cell sample circular wrappings, sample stream is under the holding of sheath fluid, formation fluid dynamics focuses on, at the ultrahigh frequency electric crystal charging after vibration of flow chamber, make sheath fluid be fractured into even dropping liquid, cell is uniformly distributed wherein, under high tension voltage under deflection, according to the state of dyeing, can different cell screenings out; The effect two of sheath fluid is the activity maintaining cell in cell screening process, so that in repeatedly analyzing maintenance cell keep the integrality of cell, requirement for the sheath fluid of input is (1). in flow cytomery process, the effect of sheath fluid mainly about bundle cell form a line by detection zone, therefore sheath fluid should be isotonic; (2). flow cytomery be visible component, therefore can not particle be had in sheath fluid; (3). flow cytomery be optical signalling (fluorescence signal), therefore can not autofluorescence be had in sheath fluid; (4). flow cytometer can be used for detecting microorganism, the bacterium therefore sheath fluid itself does not have.If sheath fluid is by bacterium or dust pollution, it is high that bacterium or dust will form the ground unrest analyzing data, affects the accuracy of data analysis, forms interference time serious to clinical and research work.Flow cytometer up-to-date at present has sheath fluid automatic filling system, and the sheath fluid of a large amount of desktop computers of China's import needs artificial, the sealing of sheath perfusion can not be ensured in operation, not only have impact on result of use, also easily occur that sheath fluid splashes down to various problems such as the pollution of burn into environment of equipment and the injuries to operator.
Summary of the invention
In order to solve the deficiencies in the prior art, the present invention is according to advanced Electromechanical Control technology, by the pipeline of sealing, driving pump, flow measurement and control assembly, sheath fluid is directly transported to the sheath liquid chamber of flow cytometer from original-pack sheath fluid tank sealing, solve the sheath fluid automatic filling problem of a large amount of bench top flow cytometer equipment that the import of present China uses, reduce the error of measurement data, improve accuracy.
The technical solution adopted for the present invention to solve the technical problems is: a kind of bench top flow cytometer sheath fluid device for casting, is made up of pipeline, rear pump, flow set, flow detection, flow indication and driver part.Wherein, described rear pump is by pipeline sticking up flow container and being connected with the liquid chamber that sticks up of flow cytometer, and described flow set, driver part, rear pump, flow detection and flow indication are electrically connected.
Described flow set for arrange need input stick up liquid measure, described driver part exports control information for the detection and flow accepting flow, exports the control electricity of driver output pump, described flow detection and flow indication, for operator provides the situation of sticking up liquid measure being input to and sticking up liquid chamber.
The invention has the beneficial effects as follows: this flow cytometer sheath fluid device for casting can stick up in perfusion process at bench top flow cytometer, avoid and stick up liquid exposure in the environment, reduce the error effect of surrounding environment to measurement data, improve the accuracy of measurement result, and it is simple to operate, improve automation mechanized operation degree, security is good, and method is simple and reliable.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is the structural drawing of a kind of flow cytometer sheath fluid device for casting of the present invention, in figure:
1. flow set, 2. driver part, 3. rear pump, 4. pipeline, 5. flow detection, 6. flow indication
Embodiment
Referring to Fig. 1, flow cytometer sheath fluid device for casting of the present invention is described in further detail.As shown in Figure 1, flow cytometer sheath fluid device for casting of the present invention is made up of flow set 1, driver part 2, rear pump 3, pipeline 4, flow detection 5 and flow indication 6.Wherein said flow set 1 is that the perfusion that sticks up of flow cytometer arranges groundwater increment, the output quantity of setting, delivers to input one end of driver part 2; The other end input signal of described driver part 2, from the output of flow detection 5, when the flow sticking up liquid conveying constantly adds up, when arriving the settings of flow set 1, the output terminal of driver part 2 disconnects the driving to rear pump 3, and rear pump 3 stops conveying sticking up liquid, once sticks up perfusion end-of-job.Wherein said, flow indication 6 show flow cytometer stick up liquid chamber inject stick up liquid measure, observe for operating personnel.
In the use of bench top flow cytometer, after additional this sheath fluid device for casting, sheath fluid is delivered directly to the sheath liquid chamber of flow cytometer from sealing state, solve the pollution problem of the bench top flow cytometer sheath perfusion of present stage a large amount of import, avoid the infringement that surrounding environment causes bench top flow cytometer, improve the accuracy of measurement result, and simple to operate, improve automation mechanized operation degree.
Claims (3)
1. a bench top flow cytometer sheath fluid device for casting, is made up of pipeline, rear pump, flow set, flow detection, flow indication and driver part.Wherein, described rear pump is by pipeline sticking up flow container and being interconnected with the liquid chamber that sticks up of flow cytometer, and described flow set, driver part, rear pump, flow detection and flow indication are electrically connected mutually.
Described flow set for arrange need input stick up liquid measure, described driver part exports control information for the detection and flow accepting flow, exports the control electricity of driver output pump, described flow detection and flow indication, for operator provides the situation of sticking up liquid measure being input to and sticking up liquid chamber.
2. a kind of bench top flow cytometer sheath fluid device for casting according to claim 1, it is characterized in that: be made up of pipeline, rear pump, flow set, flow detection, flow indication and driver part, wherein said rear pump is by pipeline sticking up flow container and being interconnected with the liquid chamber that sticks up of flow cytometer, and described flow set, driver part, rear pump, flow detection and flow indication are electrically connected mutually.
3. a kind of bench top flow cytometer sheath fluid device for casting according to claim 1, it is characterized in that: described flow set for arrange needs input stick up liquid measure, described driver part exports control information for the detection and flow accepting flow, the control electricity of driver output pump, described flow indication provides the situation of sticking up liquid measure being input to and sticking up liquid chamber for operator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410331550.1A CN105223371A (en) | 2014-07-03 | 2014-07-03 | A kind of bench top flow cytometer sheath fluid device for casting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410331550.1A CN105223371A (en) | 2014-07-03 | 2014-07-03 | A kind of bench top flow cytometer sheath fluid device for casting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105223371A true CN105223371A (en) | 2016-01-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410331550.1A Pending CN105223371A (en) | 2014-07-03 | 2014-07-03 | A kind of bench top flow cytometer sheath fluid device for casting |
Country Status (1)
| Country | Link |
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| CN (1) | CN105223371A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108627449A (en) * | 2017-03-23 | 2018-10-09 | 深圳市帝迈生物技术有限公司 | A kind of flow cytometer fluid system and measurement method |
| CN111492227A (en) * | 2017-11-27 | 2020-08-04 | 马丁·库恩 | Flow cytometer device |
-
2014
- 2014-07-03 CN CN201410331550.1A patent/CN105223371A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108627449A (en) * | 2017-03-23 | 2018-10-09 | 深圳市帝迈生物技术有限公司 | A kind of flow cytometer fluid system and measurement method |
| CN108627449B (en) * | 2017-03-23 | 2023-03-31 | 深圳市帝迈生物技术有限公司 | Flow cytometer fluid system and measuring method |
| CN111492227A (en) * | 2017-11-27 | 2020-08-04 | 马丁·库恩 | Flow cytometer device |
| CN111492227B (en) * | 2017-11-27 | 2023-06-20 | 马丁·库恩 | Flow cytometer device |
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Legal Events
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160106 |