CN105223263A - A kind of detection platform for measuring trace element in biological sample and detection method - Google Patents
A kind of detection platform for measuring trace element in biological sample and detection method Download PDFInfo
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Abstract
The present invention relates to a kind of detection platform for measuring trace element in biological sample and detection method, described detection platform comprises: by described Frozen Biological to solid-state refrigeration system; To the laser ablation system being refrigerated to solid-state biological sample and carrying out laser ablation; And be loaded into ionization in plasma for the gasoloid of the biological sample that laser ablation is obtained and detect the inductivity coupled plasma mass spectrometry system of the Determination of trace element contents in described biological sample.
Description
Technical field
The invention belongs to the Element detection field of biomaterial, be specifically related to detection platform and detection method that a kind of applicable employing LA-ICP-MS method measures trace element in biological sample.
Background technology
Inductivity coupled plasma mass spectrometry technology (ICP-MS) is come out existing 25 years so far.In the time of two more than ten years, it develops into a kind of being widely used and the analytical technology be greatly prized by rapidly.Existing thousands of the ICP-MS in the whole world, as conventional analysis means, are equipped in the laboratory of different research field.Along with the development and perfection of instrument self, it demonstrates advantageous ability in trace, ultratrace element analysis.
What in traditional analysis biological sample, the method for trace element adopted is classical Wet, micro-wave digestion or high-pressure digestion tank are cleared up, its principle utilizes the strand of the strong oxidizing property of nitric acid or hydrogen peroxide to biological sample to destroy, make it be dissolved into solution, then enter ICP-MS and detect.These class methods have higher requirement to the quantity of sample usually, generally clear up sample minimum at about 0.1g.Further, in digestion process, also unnecessary impurity element can be introduced.
Laser ablation inductively coupled plasma-mass spectrometry technology (LA-ICP-MS) is that a kind of solid sample directly introduces technology, its ultimate principle laser microbeam is focused on sample surfaces to make it corrosion vaporization, by carrier gas the particulate etched is loaded in plasma and ionizes, then detect through mass spectrometer system analysis.As a kind of introducing method being applicable to polytype solid sample, with interference less, highly sensitive inductivity coupled plasma mass spectrometry (ICP – MS) coupling, opened up the frontier of analytical technique of mass spectrum.
This its tool of LA-ICP-MS technology has the following advantages: (1) original position, analyze advantage in real time, fast; The good feature of highly sensitive, spatial resolution.(2) analyzable sample scope is very wide, nearly all sample all can degrade by laser instrument.(3) solid sample can decompose direct mensuration, improves sample introduction efficiency.(4) analysis of different modes can be taked, such as micro-zone analysis, holistic approach or probe analysis.(5) to sample almost not requirement.Above-mentioned solid sample is directly introduced technology (i.e. solid injection) and is decreased loaded down with trivial details Sample Preparation Procedure, not only to save time and can the possibility of decreasing pollution, avoid the dilution effect in solution preparation, favourable to reduction detection limit, and the Dry aerosol introducing plasma makes mass spectrum disturb comparatively solwution method less.
But, LA-ICP-MS method in analytic process more in the past adopts solid standard specimen, or adopts solution standard specimen to correct, and solid standard specimen adopts pressed powder in preparation process, the techniques such as molten sintering, then do not have good standard manufacturing method for biological sample.Because the fractionation effect etc. of the homogeneity of the repeatability in laser ablation process, solid sample, the physical property of solid sample itself and surface state, element drastically influence precision of analysis and precision.Therefore, how by the content of trace element in LA-ICP-MS method Accurate Determining biological sample, be one of the study hotspot in this field.
Summary of the invention
The present invention is intended to overcome the problem of the preparation of standard sample in the loaded down with trivial details and existing LA-ICP-MS method of detecting step in conventional wet decomposition means, the invention provides a kind of detection platform for measuring trace element in biological sample and detection method, thus the trace element in biological sample can be measured quickly and accurately.
An aspect of of the present present invention provides a kind of detection platform for measuring trace element in biological sample, and described detection platform comprises: by described Frozen Biological to solid-state refrigeration system; To the laser ablation system being refrigerated to solid-state biological sample and carrying out laser ablation; And be loaded into ionization in plasma for the gasoloid of the biological sample that laser ablation is obtained and detect the inductivity coupled plasma mass spectrometry system of the Determination of trace element contents in described biological sample.
Detection platform provided by the invention, can be effectively extremely solid-state by biological sample snap frozen, obtain the solid standard specimen being adapted at using in LA-ICP-MS method, and do not introduce any chemical reagent in testing process, environment-friendly high-efficiency, is conducive to carrying out of subsequent detection operation.Detection platform structure of the present invention is simple, easy to use, and refrigeration rapidly, can the content of trace element in Fast Measurement biological sample.
In the present invention, described refrigeration system comprises: the sample cell depositing biological sample; At top, there is opening and hold the housing of sample cell described at least one; Described opening can be sealed and the lid of the laser penetration allowing described laser ablation system to send; The refrigeration unit that is incubated is carried out for freezing biological sample; And for the temperature measuring unit of the temperature of measuring described biological sample.
Housing provided by the invention can hold multiple sample cell simultaneously, simultaneously can be undertaken freezing via refrigeration unit by multiple sample and be incubated, and is conducive to that subsequent sample detects, the carrying out rapidly of calibration steps, improves the speed of detection.Can be sealed completely with housing by lid, form closed cavity, and the laser-light transparent lid that laser ablation system sends, carries out laser ablation to the solid-state biological sample that is refrigerated to be contained in housing, thus obtains the follow-up gasoloid carrying out detecting with LA-ICP-MS method.Further, can be conducive to controlling refrigeration unit to be incubated the biological sample after freezing by arranging temperature measuring unit.
In the present invention, described refrigeration unit possesses for providing refrigerant with the refrigeration pipe of freezing biological sample and the flow control member controlling the flow velocity of the refrigerant in described refrigeration pipe according to the temperature that described temperature measuring unit detects in described housing.
Use in the present invention in housing, to provide refrigerant with the flow control member of the flow velocity of the refrigerant in the refrigeration pipe of freezing biological sample and the temperature controlled refrigerating pipeline that detects according to above-mentioned temperature measuring unit, so that freezing biological sample being incubated effectively.Preferably, refrigerant can be nitrogen, thus can freeze more quickly.
In the present invention, described refrigeration pipe comprises refrigerant-medium inlet portion and the refrigerant export department on the sidewall being located at described housing respectively and to be connected between described refrigerant-medium inlet portion and refrigerant export department and the body for loading described sample cell be arranged in described housing; And described flow control member is located at described refrigerant-medium inlet portion.
According to the present invention, flow out from refrigerant export department after the refrigerant as refrigeration source of refrigerant-medium inlet portion inflow flows through the body be located in housing.Thus effectively carry out freezing to the biological sample in the sample cell be placed on this body by the refrigerant circulated in body.Further, the flow velocity of the refrigerant that the temperature convection that can effectively be recorded based on above-mentioned temperature measuring unit by the flow control member being arranged at refrigerant-medium inlet portion is entered in housing controls, to be conducive to being incubated the biological sample after freezing.Preferably, above-mentioned tubular body can be formed as M type, thus can more uniformly freeze.
In the present invention, described temperature measuring unit comprises the temperature measurer be connected with described sample cell.
According to the present invention, the temperature measurer by being connected with sample cell detects the temperature of the biological sample in sample cell effectively, is conducive to realizing heat insulation function.Preferably, this temperature measurer can be the temperature detecting resistance be connected with sample cell.
In the present invention, described inductivity coupled plasma mass spectrometry system possesses for the icp ms providing the gas-carrier pipeline of carrier gas to the biological sample in described housing and be connected with described gas-carrier pipeline.
According to the present invention, can provide carrier gas to the biological sample in housing by gas-carrier pipeline, the gasoloid of the biological sample obtained to make laser ablation is loaded into ionization in plasma by carrier gas, to carry out subsequent detection.
In the present invention, described gas-carrier pipeline comprises carrier gas inlet portion on the sidewall being located at described housing respectively and carrier gas export department, and described carrier gas inlet portion is connected with carrier gas supply source, and described carrier gas export department is connected with described icp ms.
In the present invention, gas-carrier pipeline comprises is located at carrier gas inlet portion on the sidewall of housing and carrier gas export department respectively, institute's carrier gas inlet portion is connected to provide carrier gas in housing with carrier gas supply source, and carrier gas export department is connected with icp ms provides the gasoloid of the biological sample be loaded into by carrier gas with latter.Namely, after laser ablation, in housing, passing into carrier gas, bringing icp ms into by degrading the gasoloid obtained, to carry out subsequent detection by carrier gas.Further simplify detection method, guarantee the accuracy of testing result.Preferably, this carrier gas can be such as the inert gas of helium, argon gas and so on.More preferably, this carrier gas can be the mixed gas of helium and argon gas.
Present invention also offers a kind of detection method using above-mentioned detection platform, comprising:
(1) be refrigerated to solid-state biological sample and standard solution by via refrigeration system, carry out laser ablation process by laser ablation system, form the gasoloid of biological sample and standard solution respectively;
(2) by inductivity coupled plasma mass spectrometry system the described gasoloid obtained in step (1) is loaded in plasma and detects after ionization, directly obtain the ion-intensity values of described biological sample and standard solution, the calibration curve about ion-intensity values-concentration of described standard solution is obtained according to the ion-intensity values of described standard solution, and the content of the trace element obtained in described biological sample that converts with the ion-intensity values of described biological sample according to the relational expression obtained from described calibration curve.
In the method for the invention, owing to employing above-mentioned detection platform, can rapidly biological sample and standard solution be refrigerated to solid-state, recycling laser ablation system and inductivity coupled plasma mass spectrometry system are tested standard solution and biological sample, carry out quantitative test using standard solution as quantitative basis to biological sample.Owing to being analyze standard specimen and sample under unified low-temperature condition simultaneously, the stability of data can be guaranteed, improve the accuracy of data, thus the measured value of trace element in biological sample can be obtained quickly and accurately, and overcome the problem of the preparation of standard sample in the loaded down with trivial details and existing LA-ICP-MS method of detecting step in conventional wet decomposition means.
In the present invention, in described step (1), described laser ablation system adopts line sweep to degrade pattern, and the wavelength of laser that described laser ablation system sends is 213mm, pulsed frequency is 20Hz, and degrading aperture is 15 ~ 200 μm, and the time of degrading is 15 ~ 200 seconds.
By the selection of the parameter of above-mentioned laser ablation system, the gasoloid of biomaterial effectively can be obtained through laser ablation.
In the present invention, in described step (2), described inductivity coupled plasma mass spectrometry system is to jump the time-resolved mode image data at peak, and in described inductivity coupled plasma mass spectrometry system, RF power is 1000w, sampling depth is 9mm, working gas is argon gas, and plasma flow amount is 15.0L/ minute, and carrier gas flux is 1.4L/ minute, data acquisition time is 120 seconds, and integral time is 60 seconds.
By the selection of the parameter of above-mentioned inductivity coupled plasma mass spectrometry system, can effectively detect the gasoloid of the biomaterial obtained through laser ablation.
Beneficial effect of the present invention:
Under the refrigeration of refrigeration source, biological sample and standard solution rapid condensation are become solid-state, thus quantitative test is fast and accurately carried out to sample.Refrigerant system configurations is simple, easy to use, and refrigeration is rapid, and low temperature keeps constant.And place multi-disc sample cell can inner analysis many groups sample and standard solution at one time.Under unified low-temperature condition, analyze standard specimen and sample simultaneously, guarantee the stability of data, improve the accuracy of data.The method solves the problem of complex operation step in traditional resolution method analysis of biological samples, and does not introduce any chemical reagent, more environment-friendly high-efficiency, and the problem that reagent blank when solving sample preparation is too high, improves detection sensitivity.The method also solves LA-ICP-MS in the past and is difficult to prepare the problem of solid standard specimen in analyzing, and compare traditional LA solid standard specimen preparation, the preparation of solution standard specimen is more simple, quick, accurate.The homogeneity of standard specimen have also been obtained maximum guarantee.And various carrier gas can be filled with in the housing, reduce the interference of polyatom particle.
Accompanying drawing explanation
Fig. 1 shows the schematic diagram of the refrigeration system of the detection platform for measuring trace element in biological sample according to an example of the present invention;
The schematic diagram of sample cell of Fig. 2 for using in the refrigeration system shown in Fig. 1;
The schematic diagram of refrigeration unit of Fig. 3 for using in the refrigeration system shown in Fig. 1;
Fig. 4 adopts the detection platform for measuring trace element in biological sample of the present invention to carry out calibration curve obtained in the embodiment detected.
Embodiment
Further illustrate the present invention below in conjunction with accompanying drawing and following embodiment, should be understood that following embodiment and/or accompanying drawing are only for illustration of the present invention, and unrestricted the present invention.
Provide a kind of detection platform for measuring trace element in biological sample according to an aspect of the present invention, described detection platform comprises: by described Frozen Biological to solid-state refrigeration system; To the laser ablation system being refrigerated to solid-state biological sample and carrying out laser ablation; And be loaded into ionization in plasma for the gasoloid of the biological sample that laser ablation is obtained and detect the inductivity coupled plasma mass spectrometry system of the Determination of trace element contents in described biological sample.
Detection platform provided by the invention, can be effectively extremely solid-state by biological sample snap frozen, obtain the solid standard specimen being adapted at using in LA-ICP-MS method, and do not introduce any chemical reagent in testing process, environment-friendly high-efficiency, is conducive to carrying out of subsequent detection operation.Detection platform structure of the present invention is simple, easy to use, and refrigeration rapidly, can the content of trace element in Fast Measurement biological sample.
Fig. 1 shows the schematic diagram of the refrigeration system of the detection platform for measuring trace element in biological sample according to an example of the present invention; The schematic diagram of sample cell of Fig. 2 for using in the refrigeration system shown in Fig. 1.As depicted in figs. 1 and 2, this refrigeration system comprises: the sample cell 6 depositing biological sample; At top, there is opening and hold the housing 2 of at least one sample cell 6; Described opening can be sealed and the lid 1 of the laser penetration allowing laser ablation system to send; The refrigeration unit that is incubated is carried out for freezing biological sample; And for the temperature measuring unit of the temperature of measuring described biological sample.This laser ablation system can be laser ablation system conventional in prior art, omits the detailed description of its structure at this.
Multiple sample cell 6 can be held simultaneously in above-mentioned housing 2, can via refrigeration unit, multiple sample be carried out freezing and be incubated simultaneously.Can be sealed completely with housing 2 by lid 1, form closed cavity, such as, in the example shown in Fig. 1, be formed as cube-shaped closed cavity.And the laser-light transparent lid 1 that laser ablation system sends, carries out laser ablation to the solid-state biological sample that is refrigerated to be contained in housing 2, thus obtains the follow-up gasoloid carrying out detecting with LA-ICP-MS method.Further, can be conducive to controlling refrigeration unit to be incubated the biological sample after freezing by arranging temperature measuring unit.
Particularly, above-mentioned housing 2 adopts the stainless steel material preparation of low temperature resistant, high strength, can tolerate the low temperature of about-50 DEG C and indeformable.The material of above-mentioned lid 1 is penetrating bubble-free quartz glass, and this silica glass material allows laser energy pass and do not decay rapidly, and focuses on biological material specimens surface.
In addition, the material of above-mentioned sample cell 6 can be the aluminium nitride ceramics of polishing both surfaces, and processes the cell body 7 of smooth depression in the one side of this sample cell 6, is used for placing biological sample or standard solution.Aluminium nitride ceramics coefficient of heat conductivity is large, and strong alkali-acid resistance is not vulnerable to sample corrosion, not yielding at low temperatures cracked.The sample cell energy uniform heat conduction fast made by it, can make whole cell body Homogeneous cooling and can keep low-temperature condition preferably.And it can carry the sample of various acidity and alkalescence, and can repeatedly use, the material bodies such as other similar stainless steel and aluminium oxide of comparing reveal its excellent performance.
In addition, above-mentioned refrigeration unit possesses for providing refrigerant with the flow control member of the flow velocity of the refrigerant in the refrigeration pipe of freezing biological sample and the temperature controlled refrigerating pipeline that detects according to above-mentioned temperature measuring unit in housing 2, so that freezing biological sample being incubated effectively.In an example of the present invention, this refrigerant can be nitrogen, thus can freeze more quickly.
Particularly, the schematic diagram of the refrigeration unit used in the refrigeration system of Fig. 3 for the example shown in Fig. 1.In this example, use nitrogen as refrigeration source.As shown in figures 1 and 3, above-mentioned refrigeration pipe comprise be located at housing 2 respectively sidewall on the nitrogen input pipe 4 as refrigerant-medium inlet portion and the nitrogen efferent duct 4 ' as refrigerant export department and to be connected between nitrogen input pipe 4 and nitrogen efferent duct 4 ' and the body 10 for loading sample cell 6 be arranged in housing 2.In this example, this refrigeration pipe such as can be made up of stainless-steel tube.And flow control member 5 is located at this nitrogen input pipe 4 place.In this example, this flow control member 5 can be such as flowrate control valve.
As indicated by the arrows in fig. 3, flow out from nitrogen efferent duct 4 ' after the refrigerant of the such as nitrogen as refrigeration source of nitrogen input pipe 4 inflow flows through the body 10 be located in housing 2.Thus effectively carry out freezing to the biological sample in the sample cell 6 be placed on this body 10 by the refrigerant of circulation in body 10.Further, the flow velocity of the refrigerant that the temperature convection that can effectively be recorded based on above-mentioned temperature measuring unit by the flowrate control valve 5 being arranged at nitrogen input pipe 4 is entered in housing 2 controls, to be conducive to being incubated the biological sample after freezing.Preferably, as shown in Figure 3, above-mentioned tubular body 10 can be formed as M type, thus can more uniformly freeze.But the shape of this body 10 is not limited thereto, as long as can carry out freezing to mounting biomaterial thereon and be incubated.And can fill insulant material in right amount in the gap of this refrigeration pipe and housing 2, to ensure that low temperature is constant.
Above-mentioned refrigeration unit cardinal principle is the ability of the fast-refrigerating utilizing liquid nitrogen, and by sample and standard solution snap frozen and in required temperature, such as-25 DEG C keep steady state.Therefore the problem for solving refrigeration and insulation can take any suitable method.
Should be able to disposable placement 4 to 6 sample cells 6 in described refrigeration system.Sample cell 6 directly contacts with refrigeration unit, controls liquid nitrogen flow velocity ensure that the temperature constant of sample cell 6 is in-25 DEG C by the flowrate control valve 5 accessed at nitrogen input pipe 4 place.Place temperature detecting resistance 8 in one end of described sample cell 6, carry out temperature monitoring.Namely, the present invention using nitrogen as refrigeration source, control nitrogen flow rate with flowrate control valve 5, with temperature detecting resistance 8 monitoring temperature change, make it remain on-25 DEG C of scopes.
With reference to Fig. 2, above-mentioned temperature measuring unit can comprise the temperature measurer 8 be connected with sample cell 6.In this example, this temperature measurer 8 can be such as the temperature detecting resistance be connected with sample cell 6.
In addition, in the present invention, above-mentioned inductivity coupled plasma mass spectrometry system possesses for the icp ms providing the gas-carrier pipeline of carrier gas to the biological sample in housing 2 and be connected with this gas-carrier pipeline.This icp ms can be such as icp ms conventional in prior art, omits the detailed description of its structure at this.Can provide carrier gas to the biological sample in housing 2 by gas-carrier pipeline, the gasoloid of the biological sample obtained to make laser ablation is loaded into ionization in plasma by carrier gas, to carry out subsequent detection.
As shown in Figure 1, above-mentioned gas-carrier pipeline comprises carrier gas inlet portion 3 on the sidewall being located at housing 2 respectively and carrier gas export department 3 ', carrier gas inlet portion 3 is connected to provide carrier gas in housing 2 with diagram abridged carrier gas supply source, and carrier gas export department 3 ' is connected with diagram abridged icp ms and provides the gasoloid of the biomaterial be loaded into by carrier gas with latter, to carry out subsequent detection.
The gas be filled with in above-mentioned gas-carrier pipeline comprises carrier gas and/or blanket gas; the Main Function of gas injection is the disturbing factors such as isolated moisture, air and dust; prevent sample to volatilize at low temperatures moisture, and utilize carrier gas that the particulate degraded is brought into plasma.
Present invention also offers a kind of detection method using above-mentioned detection platform, comprising:
(1) be refrigerated to solid-state biological sample and standard solution by via refrigeration system, carry out laser ablation process by laser ablation system, form the gasoloid of biological sample and standard solution respectively;
(2) by inductivity coupled plasma mass spectrometry system the described gasoloid obtained in step (1) is loaded in plasma and detects after ionization, directly obtain the ion-intensity values of described biological sample and standard solution, the calibration curve about ion-intensity values-concentration of described standard solution is obtained according to the ion-intensity values of described standard solution, and the content of the trace element obtained in described biological sample that converts with the ion-intensity values of described biological sample according to the relational expression obtained from described calibration curve.
According to the present invention, under the refrigeration of refrigeration source, biological sample and standard solution rapid condensation are become solid-state, thus quantitative test is fast and accurately carried out to sample.Refrigerant system configurations is simple, easy to use, and refrigeration is rapid, and low temperature keeps constant.And place multi-disc sample cell 6 can inner analysis many groups sample and standard solution at one time.Under unified low-temperature condition, analyze standard specimen and sample simultaneously, guarantee the stability of data, improve the accuracy of data.The method solves the problem of complex operation step in traditional resolution method analysis of biological samples, and does not introduce any chemical reagent, more environment-friendly high-efficiency, and the problem that reagent blank when solving sample preparation is too high, improves detection sensitivity.The method also solves LA-ICP-MS in the past and is difficult to prepare the problem of solid standard specimen in analyzing, and compare traditional LA solid standard specimen preparation, the preparation of solution standard specimen is more simple, quick, accurate.The homogeneity of standard specimen have also been obtained maximum guarantee.And various carrier gas can be filled with in housing 2, reduce the interference of polyatom particle.
Further, in above-mentioned steps (1), described laser ablation system adopts line sweep to degrade pattern, and the wavelength of laser that described laser ablation system sends is 213mm, pulsed frequency is 20Hz, and degrading aperture is 15 ~ 200 μm, and the time of degrading is 15 ~ 200 seconds.Because biomaterial belongs to organic-based material, quality is softer and be rich in moisture, therefore selects the energy needing suitably to reduce laser when laser parameter, adds large spot.Make the shadow surface of laser large as much as possible, depth of shine is shallow, and can not penetrate biomaterial.Therefore, by the selection of the parameter of above-mentioned laser ablation system, effectively laser ablation can be carried out to biomaterial.
In addition, in above-mentioned steps (2), described inductivity coupled plasma mass spectrometry system is to jump the time-resolved mode image data at peak, and in described inductivity coupled plasma mass spectrometry system, RF power is 1000w, sampling depth is 9mm, working gas is argon gas, and plasma flow amount is 15.0L/ minute, and carrier gas flux is 1.4L/ minute, data acquisition time is 120 seconds, and integral time is 60 seconds.By the selection of the parameter of above-mentioned inductivity coupled plasma mass spectrometry system, can effectively detect the gasoloid of the biomaterial obtained through laser ablation.
Particularly, the present invention can adopt the trace element in LA-ICP-MS method mensuration biological sample, comprises the steps:
A) biological sample getting suitable size is directly positioned in the groove 7 of sample cell 6;
B) draw a certain amount of standard solution, be placed in another sheet sample cell 6, multi-disc sample cell 6 can be increased as required, be used for putting different biological samples or standard solution; And connect temperature detecting resistance or temperature measurer 8, multiple temperature detecting resistance or temperature measurer 8 can be connected if desired;
C) on the stainless-steel tube 10 these sample cells 6 being placed in refrigeration unit.Lid 1 is covered and seals, and be filled with helium in gas-carrier pipeline;
D) open flowrate control valve 5, pour nitrogen fast, observe the Temperature displaying of temperature measurer 8, close-25 DEG C time, reduce nitrogen pressure, and make it remain on about-25 DEG C;
E) open laser ablation system and degrade pattern with line sweep, ICP-MS is to jump the time-resolved mode image data at peak.Make calibration curve with freezing standard solution, obtain the relational expression of calibration curve.In the same way the ionic strength according to the relational expression set up and biological sample is detected to biological sample, the content of trace element in biological specimen can be calculated.
At this step e) in, particularly, the biological sample obtained through laser ablation and the gasoloid of standard solution are loaded in plasma and detect after ionization, directly obtain the ion-intensity values of biological sample and standard solution, the calibration curve about ion-intensity values-concentration of standard solution is obtained according to the ion-intensity values of standard solution, and the content of the trace element obtained in biological sample that converts according to the ion-intensity values of the relational expression biological sample obtained from calibration curve.
And above-mentioned ion-intensity values is such as by quadrupole rod isolating ions, photomultiplier detector detects and obtains, gaseous molecular or atom is clashed into exactly in simple terms with high-velocity electrons, positive ion after ionization is accelerated to import in mass analyzer, is then undertaken collecting and record by the size order of mass-to-charge ratio (m/z).
Above-mentioned detection method of the present invention can measure the trace element of biological specimen when not clearing up sample, and the result that test result and micro-wave digestion-inductively coupled plasma mass spectrometry obtain has the consistance in error range, has reasonable.
The present invention is illustrated in greater detail by specific embodiment below in conjunction with Fig. 1 to Fig. 3.
Embodiment 1
This gives a kind of laser ablation refrigeration system.Adopt sus304 stainless steel material to make the box of a upper opening as housing 2 as shown in Figure 1, make a call to two apertures in the side surface upper part of housing 2, penetrate stainless steel pipe 3,3 ' and by conduit 3,3 ' and housing 2 firm welding, ensure to seal.Wherein, conduit 3 connects diagram abridged reduction valve and helium steel cylinder, and to be filled with carrier gas and/or blanket gas, and conduit 3 ' is connected with diagram abridged icp ms.
Two holes are made a call in the side bottom of housing 2.By hollow stainless-steel tube 10, making it be bent to form M shape by hot-working, as shown in Figure 3, and ensureing that one side is smooth without tilting.This M type stainless-steel tube 10 burnishing surface is upwards put into housing 2 inner, the two ends of stainless-steel tube 10, i.e. nitrogen input pipe 4 are connected with two apertures of the bottom of housing 2 respectively with nitrogen efferent duct 4 ', and make itself and housing 2 firm welding, ensure sealing.And on this nitrogen input pipe 4 access flow operation valve 5.Can fill insulant material in right amount in the gap of this stainless-steel tube 10 and housing 2.Subsequently, the lid 1 of quartz glass as refrigeration system of the penetrating bubble-free twin polishing of a slice can be adopted, one section of rubber sheet gasket can be installed additional at lid 1 and the junction of housing 2, and with clip, lid 1 and housing 2 be sealed.
Adopt the aluminium nitride ceramics of polishing both surfaces as sample cell 6.Process the cell body 7 of smooth depression in the one side of sample cell 6, be used for placing sample or standard solution.And can pop one's head in by set temperature around cell body 7.
This application example adopts a liver specimens test Cd wherein to carry out the reliability of Authentication devices and method.
Adopt the method step of LA-ICP-MS method mensuration biological sample medium trace element as follows:
1, sample cell 6 is positioned in 5% nitric acid soaks 2h, clean and air-dry with ultrapure water after taking-up;
2, get suitable size, the wide liver specimens of long about the 0.5mm of such as about 1mm is directly positioned in the cell body 7 of sample cell 6;
3, draw each 5mL of Cd standard solution of 2 μ g/g, 4 μ g/g, 6 μ g/g, 10 μ g/g, be placed in four sample cells 6, and connect temperature detecting resistance 8;
4, these sample cells 6 are placed on stainless-steel tube 10.Lid 1 is covered and seals.And helium is filled with in carrier gas input pipe 3;
5, open nitrogen flow operation valve 5, be filled with nitrogen fast with the pressure of 0.3mpa, observe the Temperature displaying of temperature measurer 8, close-25 DEG C time, reduce nitrogen pressure to 0.1mpa, and make it remain on about-25 DEG C.Now biological sample and standard solution are all icing;
6, open laser ablation system and degrade pattern with line sweep, ICP-MS is to jump the time-resolved mode image data at peak;
7, make calibration curve with freezing standard solution, obtain the relational expression of calibration curve.In the same way sample is detected.According to the relational expression of foundation and the ionic strength of sample, the content of biological specimen medium trace element can be calculated.Wherein, first scan freezing standard solution, obtain group element data of a line, the data point of a standard solution is obtained after averaging, after testing the standard solution of several concentration respectively, draw calibration curve, then sample is tested, after obtaining sample data, calculate corresponding concentration value by calibration curve.
Wherein as follows to the Optimal Setting of parameter each in LA-ICP-MS method: the wavelength of the laser sent in laser ablation system is 213mm, and pulsed frequency is 20Hz, degrading aperture is 200 μm, and the time of degrading is 200s; In inductivity coupled plasma mass spectrometry system: RF power is 1000w, sampling depth is 9mm, and working gas is argon gas, and plasma flow amount is 15.0L/min, and carrier gas flux is 1.4L/min, and data acquisition time is 120s, and integral time is 60s.
The data recorded in described embodiment 1 list in table 1, and can obtain the calibration curve in Fig. 4 according to data in table 1.
Table 1
Associative list 1 and Fig. 4 known, standard solution line scans detection four point, 4 average after with average detected intensity level for ordinate, with the configuration concentration value of Cd for horizontal ordinate, can draw corresponding typical curve calibrating curve equation is, the equation calculating respective alignment curve is y=884.43x, and the related coefficient of calibration curve is 0.996.And sample wire scanning result is as shown in table 2.
Table 2
| First point | Second point | Thirdly | 4th point | 5th point | 6th point | 7th point | |
| Test intensity | 5322 | 7633 | 5479 | 2689 | 5834 | 2699 | 3396 |
| Scaling results | 6.02 | 8.66 | 6.19 | 3.04 | 6.60 | 3.05 | 3.84 |
Can be learnt by as above result, this method typical curve is linearly good, can understand the distribution of biological sample medium trace element clearly.And the time that the method spends is only 1/3rd of traditional analysis, and do not introduce any chemical reagent.The method solves the problem of complex operation step in traditional resolution method analysis of biological samples, and does not introduce any chemical reagent, more environment-friendly high-efficiency, and the problem that reagent blank when solving sample preparation is too high, improves detection sensitivity.The method also solves LA-ICP-MS in the past and is difficult to prepare the problem of solid standard specimen in analyzing, and compare traditional LA solid standard specimen preparation, the preparation of solution standard specimen is more simple, quick, accurate.The homogeneity of standard specimen have also been obtained maximum guarantee.
Claims (10)
1. for measuring a detection platform for trace element in biological sample, it is characterized in that, described detection platform comprises:
By described Frozen Biological to solid-state refrigeration system;
To the laser ablation system being refrigerated to solid-state biological sample and carrying out laser ablation; And
Gasoloid for biological sample laser ablation obtained is loaded into ionization in plasma and detects the inductivity coupled plasma mass spectrometry system of the Determination of trace element contents in described biological sample.
2. detection platform according to claim 1, is characterized in that, described refrigeration system comprises:
Deposit the sample cell of biological sample;
At top, there is opening and hold the housing of sample cell described at least one;
Described opening can be sealed and the lid of the laser penetration allowing described laser ablation system to send;
The refrigeration unit that is incubated is carried out for freezing biological sample; And
For measuring the temperature measuring unit of the temperature of described biological sample.
3. detection platform according to claim 2, it is characterized in that, described refrigeration unit possesses for providing refrigerant with the refrigeration pipe of freezing biological sample and the flow control member controlling the flow velocity of the refrigerant in described refrigeration pipe according to the temperature that described temperature measuring unit detects in described housing.
4. detection platform according to claim 3, it is characterized in that, described refrigeration pipe comprises refrigerant-medium inlet portion and the refrigerant export department on the sidewall being located at described housing respectively and to be connected between described refrigerant-medium inlet portion and refrigerant export department and the body for loading described sample cell be arranged in described housing; And described flow control member is located at described refrigerant-medium inlet portion.
5. detection platform according to claim 2, is characterized in that, described temperature measuring unit comprises the temperature measurer be connected with described sample cell.
6. detection platform according to claim 2, it is characterized in that, described inductivity coupled plasma mass spectrometry system possesses for the icp ms providing the gas-carrier pipeline of carrier gas to the biological sample in described housing and be connected with described gas-carrier pipeline.
7. detection platform according to claim 6, it is characterized in that, described gas-carrier pipeline comprises carrier gas inlet portion on the sidewall being located at described housing respectively and carrier gas export department, described carrier gas inlet portion is connected with carrier gas supply source, and described carrier gas export department is connected with described icp ms.
8. use a detection method for detection platform according to any one of claim 1 to 7, it is characterized in that, comprising:
(1) be refrigerated to solid-state biological sample and standard solution by via refrigeration system, carry out laser ablation process by laser ablation system, form the gasoloid of biological sample and standard solution respectively;
(2) by inductivity coupled plasma mass spectrometry system the described gasoloid obtained in step (1) is loaded in plasma and detects after ionization, directly obtain the ion-intensity values of described biological sample and standard solution, the calibration curve about ion-intensity values-concentration of described standard solution is obtained according to the ion-intensity values of described standard solution, and the content of the trace element obtained in described biological sample that converts with the ion-intensity values of described biological sample according to the relational expression obtained from described calibration curve.
9. detection method according to claim 8, it is characterized in that, in described step (1), described laser ablation system adopts line sweep to degrade pattern, and the wavelength of laser that described laser ablation system sends is 213mm, pulsed frequency is 20Hz, and degrading aperture is 15 ~ 200 μm, and the time of degrading is 15 ~ 200 seconds.
10. detection method according to claim 8 or claim 9, it is characterized in that, in described step (2), described inductivity coupled plasma mass spectrometry system is to jump the time-resolved mode image data at peak, and in described inductivity coupled plasma mass spectrometry system, RF power is 1000w, and sampling depth is 9mm, and working gas is argon gas, plasma flow amount is 15.0L/ minute, carrier gas flux is 1.4L/ minute, and data acquisition time is 120 seconds, and integral time is 60 seconds.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109668818A (en) * | 2018-12-26 | 2019-04-23 | 宁波大学 | Cell analysis apparatus and method based on laser ablation |
| CN110196275A (en) * | 2019-05-15 | 2019-09-03 | 中国科学院上海硅酸盐研究所 | It is a kind of for the high temperature real-time sample pond of laser ablation system and its detection method |
| CN112213374A (en) * | 2020-08-21 | 2021-01-12 | 苏州格目软件技术有限公司 | Microbial detection platform for aquatic plant growth environment |
| CN112229894A (en) * | 2020-08-21 | 2021-01-15 | 苏州格目软件技术有限公司 | Biological trace element detection platform for plasma charge determination |
| CN112229897A (en) * | 2020-09-11 | 2021-01-15 | 苏州格目软件技术有限公司 | Biological detection platform using charge detection |
| CN112229895A (en) * | 2020-08-21 | 2021-01-15 | 苏州格目软件技术有限公司 | Biological detection platform based on trace element determination |
| CN112362722A (en) * | 2019-07-26 | 2021-02-12 | 中国科学院上海硅酸盐研究所 | Quantitative analysis method for laser ablation inductively coupled plasma mass spectrum |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004347473A (en) * | 2003-05-22 | 2004-12-09 | Tdk Corp | Quantitative analytical method using la-icp-ms, and manufacturing method of organic substance-containing molding |
| CN102184832A (en) * | 2011-01-11 | 2011-09-14 | 西北大学 | Sample inlet device of laser ablation plasma mass spectrum |
| US20120104244A1 (en) * | 2010-11-03 | 2012-05-03 | University Of North Texas | Petroleum oil analysis using liquid nitrogen cold stage - laser ablation- icp mass spectrometry |
| CN102455317A (en) * | 2010-10-27 | 2012-05-16 | 中国石油化工股份有限公司 | Micro component laser ablation isotope analyzing device and method |
| ES2425138A1 (en) * | 2013-06-07 | 2013-10-11 | Universidad De Oviedo | Cryoablation cell with temperature control of the sample |
| CN103364483A (en) * | 2013-07-17 | 2013-10-23 | 中国科学院地质与地球物理研究所 | System and method for realizing linkage of independent instruments in mass spectrometry analysis system |
-
2014
- 2014-05-30 CN CN201410240392.9A patent/CN105223263B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004347473A (en) * | 2003-05-22 | 2004-12-09 | Tdk Corp | Quantitative analytical method using la-icp-ms, and manufacturing method of organic substance-containing molding |
| CN102455317A (en) * | 2010-10-27 | 2012-05-16 | 中国石油化工股份有限公司 | Micro component laser ablation isotope analyzing device and method |
| US20120104244A1 (en) * | 2010-11-03 | 2012-05-03 | University Of North Texas | Petroleum oil analysis using liquid nitrogen cold stage - laser ablation- icp mass spectrometry |
| CN102184832A (en) * | 2011-01-11 | 2011-09-14 | 西北大学 | Sample inlet device of laser ablation plasma mass spectrum |
| ES2425138A1 (en) * | 2013-06-07 | 2013-10-11 | Universidad De Oviedo | Cryoablation cell with temperature control of the sample |
| CN103364483A (en) * | 2013-07-17 | 2013-10-23 | 中国科学院地质与地球物理研究所 | System and method for realizing linkage of independent instruments in mass spectrometry analysis system |
Non-Patent Citations (2)
| Title |
|---|
| 杜一平: "《现代仪器分析方法》", 31 October 2008, 华东理工大学出版社 * |
| 杨红霞等: "激光剥蚀-电感耦合等离子体质谱法原位分析印度芥菜中Cd、P、S、Cu等7种元素", 《分析化学研究报告》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109668818A (en) * | 2018-12-26 | 2019-04-23 | 宁波大学 | Cell analysis apparatus and method based on laser ablation |
| CN110196275A (en) * | 2019-05-15 | 2019-09-03 | 中国科学院上海硅酸盐研究所 | It is a kind of for the high temperature real-time sample pond of laser ablation system and its detection method |
| CN110196275B (en) * | 2019-05-15 | 2022-04-05 | 中国科学院上海硅酸盐研究所 | High-temperature real-time sample pool for laser ablation system and detection method thereof |
| CN112362722A (en) * | 2019-07-26 | 2021-02-12 | 中国科学院上海硅酸盐研究所 | Quantitative analysis method for laser ablation inductively coupled plasma mass spectrum |
| CN112362722B (en) * | 2019-07-26 | 2022-07-12 | 中国科学院上海硅酸盐研究所 | Quantitative analysis method for laser ablation inductively coupled plasma mass spectrum |
| CN112213374A (en) * | 2020-08-21 | 2021-01-12 | 苏州格目软件技术有限公司 | Microbial detection platform for aquatic plant growth environment |
| CN112229894A (en) * | 2020-08-21 | 2021-01-15 | 苏州格目软件技术有限公司 | Biological trace element detection platform for plasma charge determination |
| CN112229895A (en) * | 2020-08-21 | 2021-01-15 | 苏州格目软件技术有限公司 | Biological detection platform based on trace element determination |
| CN112229897A (en) * | 2020-09-11 | 2021-01-15 | 苏州格目软件技术有限公司 | Biological detection platform using charge detection |
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