CN105219657B - Rainbow conk liquid fermentation high polysaccharide bacterial strain and its selection - Google Patents
Rainbow conk liquid fermentation high polysaccharide bacterial strain and its selection Download PDFInfo
- Publication number
- CN105219657B CN105219657B CN201510688406.8A CN201510688406A CN105219657B CN 105219657 B CN105219657 B CN 105219657B CN 201510688406 A CN201510688406 A CN 201510688406A CN 105219657 B CN105219657 B CN 105219657B
- Authority
- CN
- China
- Prior art keywords
- rainbow conk
- fermentation
- bacterial strain
- strain
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of rainbow conk liquid fermentation high polysaccharide bacterial strain and its selection, bacterial strain deposit number is CGMCC No. 10903；Classification naming：Rainbow conk, Latin name：Coriolus versicolor.Mutagenesis of the present invention goes out the rainbow conk mutagenic strain that one plant of prepared from coriolus versicolor mycelium polysaccharide yield is higher than former starting strain, and the yield for improving coriolan can create good economic benefit.
Description
Technical field
The present invention relates to a kind of rainbow conk liquid fermentation high polysaccharide bacterial strain and its selections.
Background technique
Intracellular Polysaccharide of Poly-stictus Versicolor PS-K (Coriolus versicolor intracellu-lar polysaccharides) system from
The binding protein polysaccharide of extraction purification, is good life in the mycelia of Basidiomycetes Polyporaceae rainbow conk category fungi rainbow conk or culture
Object effect regulator has and inhibits tumour, adjusts the multiple pharmacological effects such as immune, reduction Radiotherapy chemotherapy toxicity, and has anti-Chinese mugwort
Grow virus activity.Since the resource of field rainbow conk is limited, the at high cost, period using solid fermentation cultured mycelia extraction polysaccharide
Length, unstable product quality, low output, and Drug Administration of China requires to be changed to liquid training by solid culture recently
It supports prepared from coriolus versicolor mycelium and extracts intracellular polyse, so adapting to extensive intensive manufacture to make up these deficiencies.Coriolan master
It to extract to obtain from the mycelium of liquid fermentation, the research that rainbow conk liquid fermentation improves polysaccharide yield at present is mostly in rainbow conk
There are many condition of liquid fermentation and research in coriolan extraction process, and by induction mutation of bacterium, specific strain is obtained to mention
The content of high tunning coriolan, almost without report, yield is high, biological character is excellent, the good rainbow conk of the production traits
Strain is to ensure rainbow conk production and the good prerequisite of rainbow conk industry development, and the most yield of rainbow conk strain separated from field is not
Height is needed by domestication and continuous breeding, to improve the yield and the production traits of rainbow conk, therefore, the breeding side of strain
The research in face is just particularly important.The efficiency of spontaneons screening is lower, and takes the method effect of artificially breeding preferable, and rainbow conk is made
For a kind of macro fungi, the characteristics of having its own, the election effects of novel bacterial be unable to do without the Breeding of Edible Mushroom in terms of research.
Excellent medicinal fungi engineering bacteria is the key that improve medicinal fungi liquid fermentation level.Currently, to medicinal fungi
The breeding technique of use mainly has natural selection, mutation breeding and crossbreeding.Except utilization ultraviolet light, laser and chemical mutagen
Equal physics and chemistry behaviors are because usually filtering out outside excellent medicinal fungi, in order to improve the validity and purpose of medicinal fungi breeding
Property, researcher increasingly payes attention to constructing novel medicinal fungi using Protoplast Fusion Technique, gene recombination technology etc.
Fermentation.Protoplast fusion is also known as cell fusion, which is not influenced by the affinity factor, it can be achieved that medicinal fungi
Effective mixing of full-length genome in kind between different cultivars, between inter-species and category in cell, makes the biggish remote edge kind of genetic distance
Between, generic cross is possibly realized.But due to affecting the extensive of the technology there is technical difficulty in heterozygote standard of perfection
Using.
In the strain improvement of edible mushroom, usually there are many problem, yields screening period length, the workloads of such as novel bacterial
Greatly, strain improvement is inefficient etc., carries out edible fungus species breeding using conventional method, screens one plant of yield height, biology
The difficulty for the high yield new strains that character is excellent, the production traits is excellent is larger.Therefore, edible fungus species election effects field urgently
Introduce a kind of efficient newly breeding technique and technique.
At present in Microbial Breeding, is received and pursued using ion beam progress mutation breeding, ion beam is as a kind of new
Mutation source developing because of its unique mutagenic mechanism and biological effect extremely rapid in terms of mutation breeding, ion implanting can draw
The fracture of DNA key is played, causes a large amount of acceptor atom displacements, recombination, forms new molecular structure and gene, it is prominent to generate gene abundant
Become, to improve mutation rate.Compared with classic mutagenesis source, ion implanting can obtain higher mutation with lesser physiological damage
Rate, the wider spectrum of mutation, and have many advantages, such as equipment it is simple, it is easy to use, low in cost, to human body and environmentally friendly.Closely
Nian Lai has been reported that and Mutation technique induced by ion beam is used in the mutagenesis of antibiotic strain, and succeeds.Xu Liqing, Zhang Yinfen
Et al. using ion beam implantation be applied to ribostamycin, kanamycins producing strains and the anti-strain producing strains of kanamycins mutagenesis,
The result shows that titer of antibodies unit is significantly improved, generated with the N+ production bacterial strain-gentamicin handled and kanamycins
Bacterium, the superior strain selected have been used for producing, and increase rate is respectively 20% or so and 12.5% or more, and economic benefit is obvious.
Zhao Shu in 2006 is glad et al. to carry out mutagenic and breeding to a saccharomycete TQ601 using ion beam implantation, successfully obtains one plant of production
The low bacterial strain TQ105 bacterium of higher alcohol, reduces 34.15% than starting strain.Ion beam mutation technology is used for the breeding work of bacterial strain
Make, effect is better than traditional induced-mutation technique.
Few is reported for rainbow conk breeding of new variety at present, is yet there are no both at home and abroad in relation to utilizing ion beam mutation mutagenic and breeding
The relevant report of high yield coriolan fermentation strain, the implementation of this project will fill up the blank of this respect.Introduce ion beam mutagenesis
Technology application plays its advantage in the breeding of high yield coriolan fermentation strain, the success rate of mutagenic and breeding is greatly improved, in cloud
A new high efficiency method is established on sesame liquid fermentation strain breeding thereof, opens up new road for the development of fungi fermentation technology.
Summary of the invention
The purpose of the present invention is to provide a kind of rainbow conk liquid fermentation high polysaccharide bacterial strain and its selections.
The technical solution of the invention is as follows：
A kind of rainbow conk liquid fermentation high polysaccharide bacterial strain, it is characterized in that：Deposit number is CGMCC No.10903；Classification life
Name：Rainbow conk, Latin name：Coriolus versicolor.
The rainbow conk liquid fermentation high polysaccharide bacterial strain, fermentation condition are：Fermenter volume 100 L, liquid amount 60L,
Speed of agitator 150r/win, ventilatory capacity 1：0.5-1, pressure differential method are inoculated with, inoculum concentration 8%, are cultivated under the conditions of 27~28 DEG C；Addition
0.1% soya-bean oil is as defoaming agent；The fermentation termination time is 60 hours；Fermentation medium：Every liter of fermentation liquid corn flour 30g, beans
Cake powder 15g, peptone 3g, glucose 30g, carbon-nitrogen ratio 19:1.
A kind of selection of rainbow conk liquid fermentation high polysaccharide bacterial strain, it is characterized in that：Include the following steps：
(1) it selects a variety of different rainbow conk to produce bacteria strain, carries out the liquid fermentation test of rainbow conk, select prepared from coriolus versicolor mycelium
High yield starting strain of the highest rainbow conk bacterial strain of polysaccharide yield as mutagenesis；
(2) starting strain utilizes protoplast technology of preparing, carries out the preparation of rainbow conk protoplast；
(3) protoplast of rainbow conk starting strain is as low energy N+The material of ion implantation mutagenesis：Pass through rainbow conk plasm
The preparation of body, and the suspension for making rainbow conk protoplast reach 106/ml are drawn 0.1ml and are coated in blank culture dish, air-dry,
Carry out low energy N+Ion implantation mutagenesis；
(4)N+Ion implantation mutagenesis：Material in culture dish is coated on to step (3) and carries out N+ ion implantation mutagenesis, so
It is eluted afterwards with 1ml 0.6mol/L sucrose homeo-osmosis agent, absorption 0.1ml eluent is coated on regeneration plating medium and trains
It supports；
(5) screening of mutagenic strain：Plate after ion beam mutation is washed with 0.6mol/L sucrose homeo-osmosis agent
It is de-, it is coated on regeneration culture medium plate, after bacterium colony grows up to, selects sturdy dense, the faster mycelia of growth of mycelia and carry out liquid
Body fermentation test, is measured mycelium polysaccharides, the mycelium polysaccharides content rainbow conk bacterial strain higher than starting strain is selected, to it
Screening and culturing is carried out, the rainbow conk new strains of one plant of liquid fermentation high polysaccharide are finally obtained；
(6) stabilization characteristics of genetics is verified：By 20 generation of bacterial strain continuous passage after above-mentioned mutagenesis screening, liquid is then carried out
Fermentation test, the unconverted new mutagenesis rainbow conk liquid fermentation engineering being ultimately determined on producing of stable yield, character
Bacterial strain.
The N+Ion implanting conditions are that Implantation Energy is 20KeV, and implantation dosage is 9 × 1014ions/cm2, target chamber is true
Reciprocal of duty cycle is 10-3pa, with the injection of 5s pulsed, is divided into 15s.
Regeneration culture medium is：5 grams of maltose, 10 grams of glucose, 4 grams of yeast powder, 0.6mol/L mannitol, 5.5 grams of agar,
PH is naturally, it is 1000mL that distilled water, which is settled to total volume,.
Advantages of the present invention and good effect are：
1, the breeding of the edible fungus species such as previous rainbow conk generallys use the progress of the means such as crossbreeding and UV mutation,
Efficiency of inducing mutation is low, easily leads to negative mutation, the disadvantages of inhereditary feature is unstable, and by using ion implantation technique mutagenesis rainbow conk etc.
Edible mushroom protoplast carries out the mutagenic and breeding of bacterial strain, improves the efficiency of mutagenesis and the probability of direct mutation, and after mutagenesis
Rainbow conk bacterial strain is not susceptible to degenerate.The cloud that one plant of prepared from coriolus versicolor mycelium polysaccharide yield is higher than former starting strain is gone out using mutagenesis of the present invention
Sesame mutagenic strain, the yield for improving coriolan can create good economic benefit.
2, the present invention passes through the best optimal culture condition of rainbow conk new strains and optimal liquid fermentation parameter to high polysaccharide
Research carry out screening suitable high polysaccharide from the nutrition of culture medium, pH, fermentation time etc. using tunning polysaccharide as index
The best fermentation parameter of new strains improves the factorial production cloud from shaking flask culture to the different fermentations parameter of factory's fermenting and producing
The yield of sesame polysaccharide provides mature technique for the factorial production coriolan.
3, the rainbow conk new strains of one plant of liquid fermentation high polysaccharide are obtained by low energy N+ implantation mutagenesis, it is best to new strains
The research of culture medium and liquid parameter makes to ferment coriolan yield higher than high yield starting strain 50%, every liter of liquid fermentation amount bacterium
Mycelium polysaccharide reaches 2.26 grams.
Present invention process is described further below with reference to embodiment.
The method of low energy N+ implantation mutagenic and breeding disease-resistant high yield rainbow conk bacterial strain and the bacterial strain of institute's breeding, deposit number are
CGMCC No.10903；Classification naming：Rainbow conk, Latin name：Coriolus versicolor, depositary institution：The micro- life of China
Object culture presevation administration committee common micro-organisms center, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date
On 06 11st, 2015.
Specific embodiment
The process of mutagenesis screening rainbow conk liquid fermentation high polysaccharide new strains, step are：
1, high yield prepared from coriolus versicolor mycelium polysaccharide starting strain screens
From domestic rainbow conk strain SYZ1, SYZ2, SYZ3, SYZ4, SYZ5, SYZ6, SYZ7, SYZ8 for introducing different cultivars.
Respectively from the Chinese Academy of Agricultural Sciences, the bacterial strain of northeast and the preservation of this research institute is inoculated on PDA slant medium, 28 DEG C are kept away bacterial strain
It is activated within optical culture 7 days.By the strain inoculated of activation in the center that PDA culture medium culture dish is housed, record rainbow conk mycelia sprouts
The time of hair measures its speed of growth, observes the form (mycelia thickness, dense) of mycelia.Then the examination of rainbow conk liquid fermentation is carried out
It tests, is quantitatively seeded in fluid nutrient medium (culture medium prescription is seen below) by punching, 150 revs/min 28 DEG C of rotary shaker are protected from light
Culture 7 days, 4000 revs/min are centrifuged 5 minutes, collect mycelium, 65 DEG C drying to constant weight, measures its quality and obtains rainbow conk liquid fermentation
Biomass, while carrying out prepared from coriolus versicolor mycelium polysaccharide and being measured.Using prepared from coriolus versicolor mycelium polysaccharide yield soprano as coriolan
High yield starting strain.The last high yield for determining one plant of rainbow conk bacterial strain as mutagenesis using SYZ2 from the Chinese Academy of Agricultural Sciences of test sets out
Bacterial strain.(being shown in Table 1)
High yield mycelium polysaccharides starting strain screens (table 1)
2, low energy N+Ion implantation mutagenesis
(1) sample pre-treatments：Starting strain SYZ2 cultivates 5 days mycelia, is digested 5 hours at 30 DEG C with 2% lywallzyme,
Enzymolysis liquid is filtered with 8 layers of lens wiping paper, washing is filtered by several times with the total 8ml of homeo-osmosis agent, takes filtered fluid.4000r/min centrifugation
10 minutes, supernatant is removed, with homeo-osmosis agent 8ml centrifuge washing, obtains the protoplast of purifying.By protoplast osmotic pressure
Stabilizer 10ml dilution is used, and protoplast suspension is made, is counted with blood counting chamber.Purification protoplast suspension is diluted to
106A/ml.0.1ml is taken to be coated in culture dish, sterile wind air-dries, and carries out low energy N+ ion implanting immediately.
(2) ion implanting conditions：Implantation Energy is 20KeV, and implantation dosage is 9.00 × 1014ions·cm-2Target chamber vacuum
Degree is 10-3Pa is divided into 15s with the injection of 5s pulsed.(this condition is saving science and technology support project for my research team《With people
The multi-stage cross such as work mutation breeding are combined method breeding rainbow conk disease-resistant high yield new strains》In filter out after study best ion note
Enter condition)
3, the screening of prepared from coriolus versicolor mycelium polysaccharide superior strain
(1) primary dcreening operation：Plate after ion beam mutation 0.6mol/L sucrose homeo-osmosis agent is eluted, 0.1ml is taken
It is coated on regeneration culture medium plate, after bacterium colony grows up to, selects the bacterial strain for having obvious antagonism between mycelium, pick out altogether
There are 96 plants.Then be transferred on PDA culture medium plate and carry out antagonistic effect with starting strain SYZ2, filter out altogether 5 plants have it is obvious short of money
Antibacterial strain.5 plants of bacterial strains are SYZ203, SYZ209, SYZ225, SYZ261, SYZ285；(2) secondary screening：It then will be after primary dcreening operation
The test of bacterial strain rainbow conk liquid fermentation selects mycelium polysaccharides yield to be higher than high polysaccharide of the bacterial strain of control strain as mutagenesis
Bacterial strain, finally obtaining a plant height and producing the rainbow conk new strains SYZ209 number of mycelium polysaccharides is SYZ9, higher than starting strain SYZ2
Out 35.9%, it finally carries out isozyme electrophoresis and tests to determine that SYZ9 is high yield mycelia polysaccharide new strains.(being shown in Table 2)
Table 2:5 plants of dissociants with to impinging upon PD solution culture fermentation situation table
4, genetic stability is verified
L, the bacterial strain obtained after mutagenesis screening is subjected to 20 passages, rainbow conk experiment in cultivation is then carried out again, with the first generation
Compare, select mycelium polysaccharides stable yield (between the yield of i.e. each generation bacterial strain be not present significant difference, statistical analysis,
Variance p>O.05), character is unconverted as final rainbow conk mutagenicity high-yield bacterial strain.It finally screens to one plant of disease-resistant high yield rainbow conk
Mutagenic strain, stabilization characteristics of genetics higher by 35.9% than former high yield starting strain yield, deposit number are CGMCC No.10903.
5, the best optimal culture condition of rainbow conk new strains of high polysaccharide and the research of optimal liquid fermentation parameter.
Using tunning mycelium polysaccharides as index, carry out screening suitable height from the nutrition of culture medium, PH, fermentation time etc.
The best fermentation parameter for producing polysaccharide new strains improves batch production from shaking flask culture to the different fermentations parameter of factory's fermenting and producing
The yield of coriolan is produced, mature technique is provided for the factorial production coriolan.
1. the screening of high polysaccharide optimal medium formula
(influence, the pH measurement of the determination of most suitable carbon source, the determination of most suitable nitrogen source, carbon-nitrogen ratio (C/N) to fermentation)
It has been studied much in the research researcher of the most suitable carbon source of rainbow conk liquid fermentation, nitrogen source, carbon-nitrogen ratio (C/N), and
And the optimal carbon source of rainbow conk liquid fermentation, nitrogen source, carbon-nitrogen ratio (C/N) have been developed, this research team is with for many years in edible medicinal
In bacteria liquid fermentation the liquid fermentation medium formula that develops as basic fermentation medium (glucose 20g, corn flour 20g,
Beancake powder 10g, KH2PO410g, MgSO45g, VBl100mg, H2O1000ml) combine domestic some researchers in rainbow conk liquid
The research method of fermentation is screened with C/N 20 or so, carries out research sieve using tunning mycelium production, polysaccharide as index
Choosing obtains one group of rainbow conk new strains optimal medium for being suitble to high polysaccharide.Optimal medium is：(glucose 30g, corn flour
30g, beancake powder 15g, 3 grams of peptone, KH2PO410g, MgSO45g, VBl100mg, H2O1000ml 3) are shown in Table.
Table 3：8%, 140 rev/min 28 DEG C of rotary shaker of SYZ9 liquid fermentation inoculum concentration are protected from light culture 6 days
Optimal medium is：Glucose 30g, corn flour 30g, beancake powder 15g, 3 grams of peptone, KH2PO410g,
MgSO45g, VBl100mg, H2O1000ml,C:N is 19:1.
2. the research that the different parameters during from laboratory to the factorial production change
(using biomass, polysaccharide yield, most short fermentation time as the factorial production parameter)
The test of laboratory shake flask liquid fermentation
The test of laboratory shake flask liquid fermentation：New strains SYZ9 is to be formulated with optimal medium, 28 DEG C of cultivation temperature, shaking table
150 revs/min of revolving speed, inoculum concentration 8%.Shaking flask liquid fermentation test discovery is rising and then is declining again from low to high when pH value,
It is exactly best fermentation time point when reaching highest.Reduced sugar is reducing in entire fermentation process, restores glycosyl when to fermentation ends
This is without too big variation.It is 6 days that laboratory shake flask liquid fermentation, which tests Best Times, fermentation end products, and prepared from coriolus versicolor mycelium is dry
Weight 28.42g, mycelium polysaccharides content 8.6%, every liter of fermentation material can produce prepared from coriolus versicolor mycelium polysaccharide 2.41g.
Batch production 100L fermentor liquid fermentation test
Note：Due to the presence of cornstarch when fermentation does not complete, polysaccharide numerical value is higher when detecting with anthrone-sulphuric acid method.
100 liters of liquid fermentation tank tests of batch production：New strains SYZ9 is formula with optimal medium, and (ventilation adds fermentor
Stirring) volume 100L, liquid amount 60L, speed of agitator 150r/win, ventilatory capacity 1：0.5-1, pressure differential method inoculation, inoculum concentration are
8%, it cultivates under the conditions of 27~28 DEG C.0.1% soya-bean oil is added as defoaming agent.Entire fermentation process discovery and laboratory shake flask liquid
Body fermentation test is with uniformity, and 60 hours ferment tank time is best, fermentation end products, prepared from coriolus versicolor mycelium dry weight
26.58g, mycelium polysaccharides content 8.5%, every liter of fermentation material can produce prepared from coriolus versicolor mycelium polysaccharide 2.26g.
6, pass through low energy N+Injection mutagenesis obtains the rainbow conk new strains of one plant of liquid fermentation high polysaccharide, best to new strains
The research of culture medium and liquid parameter makes to ferment coriolan yield higher than high yield starting strain 50%, every liter of liquid fermentation amount bacterium
Mycelium polysaccharide reaches 2.26 grams.
Polysaccharide detection method：Anthrone-sulphuric acid method measurement
Reduced sugar detection method：The measurement of DNS method
Culture medium prescription：
Regeneration culture medium：RM4 regeneration culture medium：5 grams of maltose, 10 grams of glucose, 4 grams of yeast powder, 0.6mol/L sweet dew
Alcohol, 5.5 grams of agar, PH are naturally, it is 1000mL that distilled water, which is settled to total volume,.
PD fluid nutrient medium：Potato 200g liquor, glucose 20g, KH2PO410g, MgS ()45g, H2O1000m1。
Basic fermentation medium：Glucose 20g, corn flour 20g, beancake powder 10g, KH2PO410g, MgSO45g,
VBl100mg, H2O1000ml。
Claims (2)
1. a kind of rainbow conk bacterial strain of liquid fermentation high polysaccharide, it is characterized in that：Deposit number is CGMCC No. 10903；Classification
Name：Rainbow conk, Latin name：Coriolus versicolor.
2. the method that rainbow conk bacterial strain according to claim 1 is used for liquid fermentation high polysaccharide, it is characterized in that：Fermentation condition
For：Fermenter volume 100 L, liquid amount 60L, 150 r/win of speed of agitator, ventilatory capacity 1：0.5-1, pressure differential method inoculation, inoculation
Amount is 8%, is cultivated under the conditions of 27~28 DEG C；0.1% soya-bean oil is added as defoaming agent；The fermentation termination time is 60 hours；Fermentation
Culture medium：Every liter of fermentation liquid corn flour 30g, 15 g of beancake powder, 3 g of peptone, 30 g of glucose, carbon-nitrogen ratio 19:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510688406.8A CN105219657B (en) | 2015-10-22 | 2015-10-22 | Rainbow conk liquid fermentation high polysaccharide bacterial strain and its selection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510688406.8A CN105219657B (en) | 2015-10-22 | 2015-10-22 | Rainbow conk liquid fermentation high polysaccharide bacterial strain and its selection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105219657A CN105219657A (en) | 2016-01-06 |
| CN105219657B true CN105219657B (en) | 2018-11-23 |
Family
ID=54988929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510688406.8A Active CN105219657B (en) | 2015-10-22 | 2015-10-22 | Rainbow conk liquid fermentation high polysaccharide bacterial strain and its selection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105219657B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107177510A (en) * | 2016-03-10 | 2017-09-19 | 王大健 | A kind of fontanesia leaf shelf fungus and its purposes in medicine is prepared |
| CN113717865B (en) * | 2021-09-17 | 2023-06-09 | 北京东方红航天生物技术股份有限公司 | Ganoderma lucidum mutant strain and application thereof |
| CN113957109B (en) * | 2021-10-28 | 2022-09-06 | 江苏骏豪生物科技有限公司 | Industrial green production process of polystictus glycopeptide |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067788A (en) * | 2010-10-25 | 2011-05-25 | 江苏安惠生物科技有限公司 | Method for breeding ganoderma lucidum strain by low-energy N<+> implantation induced mutation and bred strain thereof |
| CN102399840A (en) * | 2011-11-16 | 2012-04-04 | 郑州轻工业学院 | Culture medium formulation for fermenting rainbow conk high-yield EPS and application thereof in cigarettes |
| CN103070010A (en) * | 2012-12-27 | 2013-05-01 | 陕西恒田化工有限公司 | Coriolus versicolor strain and method for extracting Coriolus versicolor glucan by utilizing fermentation products of Coriolus versicolor strain |
| CN103408645A (en) * | 2013-08-27 | 2013-11-27 | 北京贯虹科技集团有限公司 | Method for efficiently preparing polysaccharopeptide |
| CN103820428A (en) * | 2014-02-17 | 2014-05-28 | 江苏安惠生物科技有限公司 | Low energy nitrogen ion injection mutation method for breeding coriolus versicolor strain and strain bred by the method |
-
2015
- 2015-10-22 CN CN201510688406.8A patent/CN105219657B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067788A (en) * | 2010-10-25 | 2011-05-25 | 江苏安惠生物科技有限公司 | Method for breeding ganoderma lucidum strain by low-energy N<+> implantation induced mutation and bred strain thereof |
| CN102399840A (en) * | 2011-11-16 | 2012-04-04 | 郑州轻工业学院 | Culture medium formulation for fermenting rainbow conk high-yield EPS and application thereof in cigarettes |
| CN103070010A (en) * | 2012-12-27 | 2013-05-01 | 陕西恒田化工有限公司 | Coriolus versicolor strain and method for extracting Coriolus versicolor glucan by utilizing fermentation products of Coriolus versicolor strain |
| CN103408645A (en) * | 2013-08-27 | 2013-11-27 | 北京贯虹科技集团有限公司 | Method for efficiently preparing polysaccharopeptide |
| CN103820428A (en) * | 2014-02-17 | 2014-05-28 | 江苏安惠生物科技有限公司 | Low energy nitrogen ion injection mutation method for breeding coriolus versicolor strain and strain bred by the method |
Non-Patent Citations (2)
| Title |
|---|
| 云芝糖肽的液体发酵培养基的研究;林晓霞等;《生物加工过程》;20060530;第4卷(第2期);64-68 * |
| 广西产云芝的云芝多糖的研究初探;陈建红等;《大众科技》;20120831;第14卷(第8期);摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105219657A (en) | 2016-01-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106978350B (en) | Aspergillus niger and application thereof in preparation of puerarin compounds | |
| CN105112305A (en) | Red yeast rice rich in Monacolin K and preparation method of red yeast rice | |
| CN109735467B (en) | Streptomyces mutant strain for high-yield tacrolimus and application thereof | |
| CN102067788B (en) | Method for breeding ganoderma lucidum strain by low-energy N<+> implantation induced mutation and bred strain thereof | |
| CN109022293B (en) | Monascus purpureus strain, and fermentation product and fermentation method thereof | |
| CN102119631B (en) | Grifola frondosa strain for producing polysaccharide with composite raw material of rice bran and wheat bran | |
| CN105219657B (en) | Rainbow conk liquid fermentation high polysaccharide bacterial strain and its selection | |
| CN101215526B (en) | Cell culturing method for accelerating synthesis of glossy ganoderma secondary metabolite | |
| CN111876333B (en) | Lentinus edodes strain XG-3 capable of producing polysaccharide at high yield and application thereof | |
| CN112430549A (en) | Natural bacterial strain for producing pullulan and application thereof | |
| CN113583881B (en) | Penicillium strain for producing brefeldin A by fermentation and application thereof | |
| CN102559513B (en) | High-yield Irpex lacteus mutant strain and culture method thereof | |
| CN108841889B (en) | Method for producing griseofulvin serving as major component of tranexamycin by microbial fermentation | |
| CN102766663B (en) | Preparation method of active polysaccharides from phellinus linteus | |
| CN109097407B (en) | Method for producing cercosporin | |
| CN108823110B (en) | Strain for producing griseofulvin and application thereof | |
| CN117187076A (en) | Paecilomyces hepialid mutagenesis strain with high adenosine yield and preparation method and application thereof | |
| CN107916198A (en) | It is a kind of effectively to suppress the microbial bacterial agent that native monosodium glutamate is formed in yeast | |
| CN103805593B (en) | Method for injecting low-energy N＜+＞ for mutation breeding hericium erinaceus strain and bred strain | |
| CN105505798A (en) | Endophytic fungus for generating ergosterol and application of endophytic fungus | |
| CN113717892B (en) | Streptomyces tsukubaensis strain for producing tacrolimus through fermentation and application thereof | |
| CN107779402B (en) | Screening method of echinocandin B production strain high-yield strain | |
| CN107686818A (en) | A kind of grifolan producing strains and grifolan preparation method | |
| CN107641602A (en) | One plant of candida utili and its fermentation lay eggs it is white in application | |
| CN103820428B (en) | Mental retardation N+inject method and the bacterial strain of institute's selection-breeding of mutagenic and breeding Coriolous Dersicolor (Fr.) Quel bacterial strain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |