CN105213295B - A kind of recombination human source collagen protein face and preparation method thereof - Google Patents
A kind of recombination human source collagen protein face and preparation method thereof Download PDFInfo
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 63
- 108010035532 Collagen Proteins 0.000 title claims abstract description 63
- 238000005215 recombination Methods 0.000 title claims abstract description 45
- 230000006798 recombination Effects 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 229920001436 collagen Polymers 0.000 claims abstract description 45
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 24
- 150000002148 esters Chemical class 0.000 claims abstract description 24
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 150000003384 small molecules Chemical class 0.000 claims abstract description 16
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 12
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- 229960003237 betaine Drugs 0.000 claims abstract description 12
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000008367 deionised water Substances 0.000 claims abstract description 12
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 12
- 235000011187 glycerol Nutrition 0.000 claims abstract description 12
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims abstract description 12
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- 229920001285 xanthan gum Polymers 0.000 claims abstract description 12
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims abstract description 11
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims abstract description 11
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229920006037 cross link polymer Polymers 0.000 claims abstract description 11
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims abstract description 11
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 claims abstract description 10
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 claims abstract description 10
- 229940106189 ceramide Drugs 0.000 claims abstract description 10
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 claims abstract description 10
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241001506047 Tremella Species 0.000 claims abstract description 9
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims abstract description 8
- 108010067770 Endopeptidase K Proteins 0.000 claims description 36
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- SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 claims description 11
- 229940101267 panthenol Drugs 0.000 claims description 11
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 229920002690 Polyoxyl 40 HydrogenatedCastorOil Polymers 0.000 claims description 4
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- 208000002874 Acne Vulgaris Diseases 0.000 description 2
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 240000008821 Menyanthes trifoliata Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
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- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
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- 241000204031 Mycoplasma Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
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- 244000309465 heifer Species 0.000 description 1
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- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
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- 150000004702 methyl esters Chemical class 0.000 description 1
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- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
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- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Organic Chemistry (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of recombination human source collagen protein face and preparation method thereof, the preparation method of the recombination human source collagen protein face comprises the following steps：Recombination human source collagen protein face is by carrier and mask matrix solution composition, after 30 alkylol acrylamide acid esters cross-linked polymers of acrylic acid (ester) class/C10, dried tremella extract, hamamelis extract, ceramide, xanthans, hyaluronic acid, deionized water, glycerine and butanediol are dispersed with stirring completely, fully swelling 12h, methyl hydroxybenzoate, glycine betaine, small molecule recombination human source collagen peptide, EDETATE SODIUM are added into system, it is dispersed with stirring completely, 50 DEG C are heated to, 30 minutes are kept the temperature under vacuum.The effective anti-aging of the present invention, wrinkle reduction, increase skin elasticity, keeps the young state of skin.
Description
Technical field
The present invention relates to a kind of recombination human source collagen protein face and preparation method thereof.
Background technology
Urban woman is often in face of the immense pressure of work and life, along with the serious pollution of environment, skin by
Very big injury, shows as that skin of face is intensely dark, pore is thick, easily raw whelk, and microgroove increases, and accelerates aging, to wide
Big people seeking beauty brings very big puzzlement.Facial mask is one kind in daily cosmetics, it is to be attached to face by the of short duration time
Portion, temporarily isolation extraneous air and pollutant, substantial amounts of nutrition and moisture are provided for skin, skin is become soft beautiful,
High resilience.
It is more in order to have the function of facial mask, it is often necessary to which that adding various active materials makes facial mask reach more preferable U.S.
In vain, moisturizing, anti-aging, anti-acne and other effects.Since collagen contains the hydrophilic radicals such as more amino and hydroxyl, Neng Gouyou
Effect realizes water lock, the effect of moisturizing.Meanwhile collagen can synthesize endogenous collagen by percutaneous absorption, wrinkle reduction, makes skin
High resilience.In addition, collagen plays the role of ultraviolet injury and whelk certain reparation and treatment, and it is added to
The features such as good compatibility, stability and antibiotic property are shown in cosmetics.But existing animal sources collagen class face
The absorbability and security of film are poor, it is impossible to very effectively play a role, and consumer cannot be safe to use.
Recombination human source collagen is that the tripeptides repetitive sequence based on 1 chain collagen domain Gly-X-Y of human III type collagen α is special
Sign, successfully constructs pichia yeast genetic engineering bacteria by the method for MOLECULE DESIGN and gene chemical synthesis, carries out high density fermentation, obtain
Cell exocrine type destination protein is obtained, there is following characteristic：
（1）Low immunogenicity：Since recombination human source collagen is the gene sequence characteristic structure according to human collagen albumen
Build, and also carried out specific screening and modification in experimentation to sequence, will easily cause the color ammonia of organism immune response
The amino residues such as acid foreclose.
（2）Virus-free hidden danger：Animal sources collagen there is one it is fatal the shortcomings that be exactly in the presence of viral hidden danger, such as fowl
Influenza virus, crazy heifer disease virus, aftosa, mycoplasma and Chlamydia etc..The recombination human source collagen obtained by gene engineering research
Albumen then overcomes this weakness, its expression system is saccharomycete, and whole experiment process is sterile working, therefore obtain
Collagen will not carry exogenous virus.
（3）Good water solubility：Maxima solubility is easy to be absorbed by organisms up to 20%.
（4）Extraction process is simple, and purity of protein is high：Recombination human source collagen is cell exocrine type albumen, without right
Thalline carries out break process, it is only necessary to isolation and purification is carried out to fermented supernatant fluid, and the species of cell exocrine type albumen is few,
The higher destination protein of readily available purity.
（5）Definite molecular structure：Pointed decoration and digestion can be carried out, most accurate repair is played to skin.
Acted on by carrying out specific digestion to RHC, generate micromolecular collagen active peptide, be conducive to the absorption of skin
With the generation of endogenous collagen, microgroove is reduced.Therefore, it is appointing for the present invention to develop a kind of recombination human source collagen protein face
Where business, it is desirable to provide one kind has effects that stronger anti-wrinkle, anti-acne, moisturizing and nourishes cosmetics.
The content of the invention
In order to overcome traditional collagen class facial mask there are the defects of, the present invention provide it is a kind of have multiple moistening effect,
Effective wrinkle reduction, keep elasticity of skin, and removing acne, safe, absorbability is good, hardly causes immunity of organism anti-
Should, the recombination human source collagen protein face of efficient water lock and preparation method thereof.
The present invention is to solve above-mentioned technical problem by following technical proposals：A kind of recombination human source collagen face
Film, it is characterised in that it is by SEQ ID NO:Amino acid sequence composition shown in 1, containing 599 amino acid, molecular weight is
55.0 kDa。
The present invention also provides a kind of preparation method of recombination human source collagen protein face, it is characterised in that it includes following
Step：Recombination human source collagen protein face is by carrier and mask matrix solution composition, and mask matrix solution is by following weight percentage
The raw material of ratio is made：0.02~2 part of small molecule recombination human source collagen peptide, 1~3 part of hamamelis extract, hyaluronic acid
0.01~0.5 part, 0.01~1 part of ceramide, 0.5~2 part of glycine betaine, 1~2 part of panthenol, dried tremella extract 0.05~0.2
Part, acrylic acid (ester) class/0.1~1 part of C10-30 alkylol acrylamide acid esters cross-linked polymer, 0.1~1 part of xanthans, glycerine 3~5
Part, 1~3 part of butanediol, 0.1~0.2 part of methyl hydroxybenzoate, 0.03~0.05 part of EDETATE SODIUM, 0.04~0.4 part of sodium hydroxide,
0.5~1 part of Cremophor RH40,75~80 parts of deionized water；Under the conditions of GMP production environments, the facial mask preparation side
Method is carried out according to following processing steps：Acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, white fungus are extracted
After thing, hamamelis extract, ceramide, xanthans, hyaluronic acid, deionized water, glycerine and butanediol are dispersed with stirring completely,
Fully swelling 12h, methyl hydroxybenzoate, glycine betaine, small molecule recombination human source collagen peptide, EDETATE SODIUM are added into system, is stirred
Mix and disperse completely, to be heated to 50 DEG C, 30 minutes are kept the temperature under vacuum；Sodium hydroxide is added into above-mentioned solution, adjusts pH value, afterwards
Cremophor RH40 is added, is dispersed with stirring completely；When solution temperature is down to 20 DEG C, panthenol is added, is stirred under vacuum scattered
Uniformly；By the material censorship of gained, sample is packed out after being filtered by 300 mesh after QB/T2872 standard test qualifications.
Preferably, the preparation method of the small molecule recombination human source collagen peptide is as follows：With SEQ ID NO:Shown in 1
RHC be raw material, be configured to the protein solution of 10~20 g/L, add the pH to 8 that 1M NaOH solutions adjust solution while stirring
~9,50 DEG C of heating water baths, add 0.1~2% 2~5 h of compound protein enzyme effect, 90 are raised the temperature to after the completion of digestion
DEG C, 15min is maintained, is separated, collected supernatant under the conditions of high speed centrifugation, is that molecular weight is the small of 204~2729 Da
Molecule RHC albumen peptide solutions, vacuum freeze drying obtain finished product.
Preferably, the compound protease is Proteinase K and trypsase is 1 in mass ratio:5~15 ratio carries out
It is compound.
Preferably, the material of the carrier is one kind in non-woven fabrics, silk or biological fiber.
The positive effect of the present invention is：The effective anti-aging of the present invention, wrinkle reduction, increase skin elasticity, keeps
The young state of skin.The effective anti-acne of the present invention, replenishing collagen, repairs damage skin, eliminates slight scar.The present invention is potent
Moisturizing, compact skin.The present invention is safe, seldom causes allergic reaction.The present invention carrier medium for non-woven fabrics, silk or
In biological fiber, production environment is 100,000 grades of GMP, local ten thousand grades, the working condition of pharmaceutical grade, safe, pollution less, can
It is safe to use.
Embodiment
More specific description is carried out to the present invention by following embodiments, but the present invention is not restricted by the embodiments.
The recombination human source collagen（Recombinant Human-source Collagen, RHC）By SEQ ID
NO:Amino acid sequence composition shown in 1, containing 599 amino acid, molecular weight is 55.0 kDa.The present invention first to RHC into
Row fixed point digestion, forms small molecule recombination human source collagen peptide.Facial mask of the present invention is by carrier and mask matrix solution
Composition, the mask matrix solution contain following component：Deionized water, glycerine, butanediol, hyaluronic acid, small molecule recombined human
Source collagen peptide, ceramide, panthenol, hamamelis extract, dried tremella extract, glycine betaine, xanthans, acrylic acid
(ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, methyl hydroxybenzoate, EDTA disodiums, sodium hydroxide, PEG-40 hydrogenation castors
Sesame oil.This kind of facial mask can supplement endogenous collagen, long-acting performance water lock moisture-keeping efficacy, make clear skin, water profit, thin
It is tender, while effective removing acne.
Embodiment 1：Proteinase K combines trypsase and carries out digestion to restructuring human-like collagen
Implementation：Recombination human source collagen is configured to the solution of 10g/L, after adjusting pH to 9.0, temperature 50 C,
It is 0.1% to add Proteinase K amount, and tryptose enzyme amount is 1%, hydrolyzes 3h.In reaction process, tieed up by the way that 1 M NaOH solutions are added dropwise
Hold pH value.90 DEG C are raised the temperature to after the completion of reaction, maintains the enzyme deactivation of 15min to react, after cooling under the conditions of high speed centrifugation
Separated, collect supernatant carry out vacuum freeze drying.By carrying out HPLC detections to sample, the glue obtained after digestion is determined
The molecular weight ranges of former protein peptides are 204-2729 Da.
Embodiment 2：The preparation method of recombination human source collagen protein face
Recombination human source collagen protein face is by carrier and mask matrix solution composition, and mask matrix solution is by following weight hundred
The raw material of ratio is divided to be made：2 parts of small molecule recombination human source collagen peptide, 3 parts of hamamelis extract, 0.5 part of hyaluronic acid, nerve
1 part of acid amides, 2 parts of glycine betaine, 1 part of panthenol, 0.2 part of dried tremella extract, acrylic acid (ester) class/C10-30 alkylol acrylamides acid esters crosslinking
1 part of polymer, 1 part of xanthans, 5 parts of glycerine, 1 part of butanediol, 0.1~0.2 part of methyl hydroxybenzoate, 0.05 part of EDETATE SODIUM, hydrogen-oxygen
Change 0.04 part of sodium, 0.5 part of Cremophor RH40,75 parts of deionized water.Using above-mentioned Formulation Implementation production technology：By propylene
Sour (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, dried tremella extract, hamamelis extract, ceramide, xanthan
After glue, hyaluronic acid, deionized water, glycerine and butanediol are dispersed with stirring completely, 12h is fully swollen, oxybenzene is added into system
Methyl esters, glycine betaine, small molecule recombination human source collagen peptide, EDETATE SODIUM, are dispersed with stirring completely, are heated to 50 DEG C, under vacuum
Insulation 30 minutes.Sodium hydroxide is added into above-mentioned solution, adjusts pH value, adds Cremophor RH40, stirring point afterwards
Clear complete.When solution temperature is down to 20 DEG C, panthenol is added, vacuum stirring is uniformly dispersed；By the material censorship of gained, by QB/
After T2872 standard test qualifications sample is packed out after the filtering of 300 mesh.
Embodiment 3：The preparation method of recombination human source collagen protein face
Recombination human source collagen protein face is by carrier and mask matrix solution composition, and mask matrix solution is according to following quality
Percentage formula weighs each component：0.02 part of small molecule recombination human source collagen peptide, 1 part of hamamelis extract, hyaluronic acid
0.02 part, 0.02 part of ceramide, 0.5 part of glycine betaine, 2 parts of panthenol, 0.1 part of dried tremella extract, acrylic acid (ester) class/C10-30
0.15 part of alkylol acrylamide acid esters cross-linked polymer, 0.1 part of xanthans, 4 parts of glycerine, 3 parts of butanediol, 0.1 part of methyl hydroxybenzoate, EDTA
0.03 part of disodium, 0.06 part of sodium hydroxide, 0.9 part of Cremophor RH40,80 parts of deionized water.Using above-mentioned Formulation Implementation
Production technology：By acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, dried tremella extract, hamamelis extract,
After ceramide, xanthans, hyaluronic acid, deionized water, glycerine and butanediol are dispersed with stirring completely, 12h, Xiang Ti are fully swollen
Methyl hydroxybenzoate, glycine betaine, small molecule recombination human source collagen peptide, EDETATE SODIUM are added in system, is dispersed with stirring completely, is heated to
50 DEG C, 30 minutes are kept the temperature under vacuum.Sodium hydroxide is added into above-mentioned solution, adjusts pH value, adds PEG-40 hydrogenation castors afterwards
Sesame oil, is dispersed with stirring completely.When solution temperature is down to 20 DEG C, panthenol is added, vacuum stirring is uniformly dispersed；By the material of gained
Censorship, sample is packed out after being filtered by 300 mesh after QB/T2872 standard test qualifications.
Embodiment 4：The preparation method of recombination human source collagen protein face
Recombination human source collagen protein face is by carrier and mask matrix solution composition, and mask matrix solution is by following weight hundred
The raw material of ratio is divided to be made：1 part of small molecule recombination human source collagen peptide, 2 parts of hamamelis extract, 0.3 part of hyaluronic acid, nerve
0.3 part of acid amides, 1 part of glycine betaine, 1.5 parts of panthenol, 0.1 part of dried tremella extract, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters
0.5 part of cross-linked polymer, 0.5 part of xanthans, 4 parts of glycerine, 2 parts of butanediol, 0.15 part of methyl hydroxybenzoate, 0.04 part of EDETATE SODIUM,
0.2 part of sodium hydroxide, 0.8 part of Cremophor RH40,78 parts of deionized water；Under the conditions of GMP production environments, the facial mask
Preparation method is carried out according to following processing steps：By acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, white fungus
Extract, hamamelis extract, ceramide, xanthans, hyaluronic acid, deionized water, glycerine and butanediol have been dispersed with stirring
Quan Hou, is fully swollen 12h, and methyl hydroxybenzoate, glycine betaine, small molecule recombination human source collagen peptide, EDTA bis- are added into system
Sodium, is dispersed with stirring completely, is heated to 50 DEG C, 30 minutes are kept the temperature under vacuum；Sodium hydroxide is added into above-mentioned solution, adjusts pH
Value, adds Cremophor RH40 afterwards, is dispersed with stirring completely；When solution temperature is down to 20 DEG C, panthenol is added, vacuum is stirred
Mix and be uniformly dispersed；By the material censorship of gained, sample is packed out after being filtered by 300 mesh after QB/T2872 standard test qualifications.
The present invention can reduce irritation of the facial mask to skin, safe and reliable, reduction skin allergy.At the same time by a variety of moisturizings
Agent collocation uses, potent performance moisturizing water lock effect.Moreover, the potent performance anti-aging effects of the addition of recombination human source collagen egg.
One aspect of the present invention is related to a kind of recombination human source collagen（The referred to as recombination human source collagen egg of the present invention
In vain）, it is by SEQ ID NO:Amino acid sequence composition shown in 1.
SEQ ID NO : 1
GPPGEPGNPG SPGNQGQPGN KGSPGNPGQP GNEGQPGQPG QNGQPGEPGS NGPQGSQGNP
GKNGQPGSPG SQGSPGNQGS PGQPGNPGQP GEQGKPGNQG PAGEPGNPGS PGNQGQPGNK
GSPGNPGQPG NEGQPGQPGQ NGQPGEPGSN GPQGSQGNPG KNGQPGSPGS QGSPGNQGSP
GQPGNPGQPG EQGKPGNQGP AGEPGNPGSP GNQGQPGNKG SPGNPGQPGN EGQPGQPGQN
GQPGEPGSNG PQGSQGNPGK NGQPGSPGSQ GSPGNQGSPG
QPGNPGQPGE QGKPGNQGPA GEPGNPGSPG NQGQPGNKGS
PGNPGQPGNE GQPGQPGQNG QPGEPGSNGP QGSQGNPGKN GQPGSPGSQG SPGNQGSPGQ
PGNPGQPGEQ GKPGNQGPAG EPGNPGSPGN QGQPGNKGSP GNPGQPGNEG QPGQPGQNGQ PGEPGSNGPQ
GSQGNPGKNG QPGSPGSQGS PGNQGSPGQP GNPGQPGEQG KPGNQGPAGE PGNPGSPGNQ GQPGNKGSPG
NPGQPGNEGQ PGQPGQNGQP GEPGSNGPQG SQGNPGKNGQ PGSPGSQGSP GNQGSPGQPG NPGQPGEQGK
PGNQGPAGG
The recombination human source collagen of the present invention is the claim of Chinese patent application Publication No. CN 102443057A
Recombination human source collagen described in 1, it can be disclosed disclosed in CN 102443057A using such as Chinese patent application
It is prepared by gene engineering expression method.
Table 1 represents recombination human source collagen through Proteinase K（Proteinase K）And trypsase（Trypsin）Jointly
The length and molecular weight of theoretical peptide fragment after digestion.
| Clipped position | Enzyme | Sequence after shearing | Peptide chain length | Molecular weight |
| 5 | Proteinase K | GPPGE | 5 | 455.468 |
| 21 | Trypsin | PGNPGSPGNQGQPGNK | 16 | 1505.567 |
| 33 | Proteinase K | GSPGNPGQPGNE | 12 | 1110.105 |
| 47 | Proteinase K | GQPGQPGQNGQPGE | 14 | 1350.367 |
| 62 | Trypsin | PGSNGPQGSQGNPGK | 15 | 1381.424 |
| 92 | Proteinase K | NGQPGSPGSQGSPGNQGSPGQPGNPGQPGE | 30 | 2728.744 |
| 102 | Proteinase K | QGKPGNQGPA | 10 | 953.022 |
| 104 | Proteinase K | GE | 2 | 204.183 |
| 120 | Trypsin | PGNPGSPGNQGQPGNK | 16 | 1505.567 |
| 132 | Proteinase K | GSPGNPGQPGNE | 12 | 1110.105 |
| 146 | Proteinase K | GQPGQPGQNGQPGE | 14 | 1350.367 |
| 161 | Trypsin | PGSNGPQGSQGNPGK | 15 | 1381.424 |
| 191 | Proteinase K | NGQPGSPGSQGSPGNQGSPGQPGNPGQPGE | 30 | 2728.744 |
| 201 | Proteinase K | QGKPGNQGPA | 10 | 953.022 |
| 203 | Proteinase K | GE | 2 | 204.183 |
| 219 | Trypsin | PGNPGSPGNQGQPGNK | 16 | 1505.567 |
| 231 | Proteinase K | GSPGNPGQPGNE | 12 | 1110.105 |
| 245 | Proteinase K | GQPGQPGQNGQPGE | 14 | 1350.367 |
| 260 | Trypsin | PGSNGPQGSQGNPGK | 15 | 1381.424 |
| 290 | Proteinase K | NGQPGSPGSQGSPGNQGSPGQPGNPGQPGE | 30 | 2728.744 |
| 300 | Proteinase K | QGKPGNQGPA | 10 | 953.022 |
| 302 | Proteinase K | GE | 2 | 204.183 |
| 318 | Trypsin | PGNPGSPGNQGQPGNK | 16 | 1505.567 |
| 330 | Proteinase K | GSPGNPGQPGNE | 12 | 1110.105 |
| 344 | Proteinase K | GQPGQPGQNGQPGE | 14 | 1350.367 |
| 359 | Trypsin | PGSNGPQGSQGNPGK | 15 | 1381.424 |
| 389 | Proteinase K | NGQPGSPGSQGSPGNQGSPGQPGNPGQPGE | 30 | 2728.744 |
| 399 | Proteinase K | QGKPGNQGPA | 10 | 953.022 |
| 401 | Proteinase K | GE | 2 | 204.183 |
| 417 | Trypsin | PGNPGSPGNQGQPGNK | 16 | 1505.567 |
| 429 | Proteinase K | GSPGNPGQPGNE | 12 | 1110.105 |
| 443 | Proteinase K | GQPGQPGQNGQPGE | 14 | 1350.367 |
| 458 | Trypsin | PGSNGPQGSQGNPGK | 15 | 1381.424 |
| 488 | Proteinase K | NGQPGSPGSQGSPGNQGSPGQPGNPGQPGE | 30 | 2728.744 |
| 498 | Proteinase K | QGKPGNQGPA | 10 | 953.022 |
| 500 | Proteinase K | GE | 2 | 204.183 |
| 516 | Trypsin | PGNPGSPGNQGQPGNK | 16 | 1505.567 |
| 528 | Proteinase K | GSPGNPGQPGNE | 12 | 1110.105 |
| 542 | Proteinase K | GQPGQPGQNGQPGE | 14 | 1350.367 |
| 557 | Trypsin | PGSNGPQGSQGNPGK | 15 | 1381.424 |
| 587 | Proteinase K | NGQPGSPGSQGSPGNQGSPGQPGNPGQPGE | 30 | 2728.744 |
| 597 | Proteinase K | QGKPGNQGPA | 10 | 953.022 |
| 599 | End of sequence | GG | 2 | 132.119 |
Above-described embodiment is the description of the invention, is not limitation of the invention, any that the present invention is simply become
Scheme after changing belongs to protection scope of the present invention.
Claims (1)
1. a kind of preparation method of recombination human source collagen protein face, it is characterised in that it is by SEQIDNO:Amino acid shown in 1
Sequence forms, and contains 599 amino acid, molecular weight 55.0kDa；
It comprises the following steps：Recombination human source collagen protein face is by carrier and mask matrix solution composition, mask matrix solution
It is made of the raw material of following weight percentage：0.02~2 part of small molecule recombination human source collagen peptide, hamamelis extract 1~3
Part, 0.01~0.5 part of hyaluronic acid, 0.01~1 part of ceramide, 0.5~2 part of glycine betaine, 1~2 part of panthenol, dried tremella extract
0.05~0.2 part, acrylic acid (ester) class/0.1~1 part of C10-30 alkylol acrylamide acid esters cross-linked polymer, 0.1~1 part of xanthans,
3~5 parts of glycerine, 1~3 part of butanediol, 0.1~0.2 part of methyl hydroxybenzoate, 0.03~0.05 part of EDETATE SODIUM, sodium hydroxide 0.04
~0.4 part, 0.5~1 part of Cremophor RH40,75~80 parts of deionized water；Under the conditions of GMP production environments, the face
Membrane preparation method is carried out according to following processing steps：By acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, silver
Ear extract, hamamelis extract, ceramide, xanthans, hyaluronic acid, deionized water, glycerine and butanediol are dispersed with stirring
After completely, 12h is fully swollen, methyl hydroxybenzoate, glycine betaine, small molecule recombination human source collagen peptide, EDTA are added into system
Disodium, is dispersed with stirring completely, is heated to 50 DEG C, 30 minutes are kept the temperature under vacuum；Sodium hydroxide is added into above-mentioned solution, adjusts pH
Value, adds Cremophor RH40 afterwards, is dispersed with stirring completely；When solution temperature is down to 20 DEG C, panthenol is added, vacuum is stirred
Mix and be uniformly dispersed；By the material censorship of gained, sample is packed out after being filtered by 300 mesh after QB/T2872 standard test qualifications；
The preparation method of the small molecule recombination human source collagen peptide is as follows：With SEQIDNO:RHC shown in 1 is raw material, is matched somebody with somebody
The protein solution of 10~20g/L is made, adds the pH to 8~9 that 1MNaOH solution adjusts solution while stirring, 50 DEG C of water-baths add
Heat, adds 0.1~2% compound protein enzyme effect 3h, and 90 DEG C are raised the temperature to after the completion of digestion, maintains 15min, at a high speed
Separated, collected supernatant under centrifugal condition, be the small molecule RHC albumen peptide solutions that molecular weight is 204~2729Da, very
Vacuum freecing-dry obtains finished product；
The compound protease is Proteinase K and trypsase is 1 in mass ratio:5~15 ratio is for composite；
The material of the carrier is one kind in non-woven fabrics, silk or biological fiber.
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| CN107320355A (en) * | 2017-07-27 | 2017-11-07 | 广州赛莱拉干细胞科技股份有限公司 | A kind of collagen protein face and preparation method thereof |
| CN107362129B (en) * | 2017-08-23 | 2019-10-18 | 广东丸美生物技术股份有限公司 | Skin matrix, preparation method and applications and cosmetics and preparation method thereof |
| CN110179690A (en) * | 2019-06-12 | 2019-08-30 | 杭州蓝朗生物技术有限公司 | A kind of collagen modified form facial mask and preparation method thereof |
| CN110934787B (en) * | 2019-11-27 | 2022-04-19 | 陈少威 | Anti-aging composition and preparation method thereof |
| CN112043643A (en) * | 2020-09-29 | 2020-12-08 | 代晓娟 | Preparation method of natural anti-aging moisturizing mask based on human collagen |
| CN113397998A (en) * | 2021-07-08 | 2021-09-17 | 江苏江山聚源生物技术有限公司 | Application of recombinant humanized collagen in preparation of anti-skin-aging products |
| CN115737506B (en) * | 2022-12-16 | 2023-09-05 | 江苏亨瑞生物医药科技有限公司 | A facial mask containing collagen and its preparation method |
| CN117205366B (en) * | 2023-11-07 | 2024-01-02 | 南京东万生物技术有限公司 | Collagen-hyaluronic acid composite hydrogel for facial filling and preparation method thereof |
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