CN105211118A - A kind of bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester - Google Patents
A kind of bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester Download PDFInfo
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- CN105211118A CN105211118A CN201410299923.1A CN201410299923A CN105211118A CN 105211118 A CN105211118 A CN 105211118A CN 201410299923 A CN201410299923 A CN 201410299923A CN 105211118 A CN105211118 A CN 105211118A
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- gentrin knotweed
- bacterium ester
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Abstract
The invention discloses a kind of bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester, bactericidal composition containing active components A and active component B, wherein active components A is selected from Gentrin Knotweed P.E, active component B is selected from any one compound following: ZJ0712, azoles bacterium ester, and the weight ratio of active components A and active component B is 1 ︰ 80 ~ 60 ︰ 1.The multiple diseases of the present composition to harm agricultural production has synergistic effect, and decrease pesticide usage amount, reduce the residual quantity of agricultural chemicals on crop, alleviate environmental pollution, to person poultry safety, Environmental compatibility is good, and disease not easily develops immunity to drugs.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of application of bactericidal composition on corps diseases containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester.
Background technology
Mainly comprising archen and Physcion in Gentrin Knotweed P.E, is viscous pasty state, generally easily makes liquid preparation, but usually because containing organic solvent, easily occurring poisoning in liquid preparation, may be used for preventing and treating powdery mildew of cucumber.
ZJ0712, azoles bacterium ester all belong to methoxy acrylic bactericide, are widely used in the disease of crops.
But in the real process of agricultural production, the problem that controlling disease the most easily produces is the generation of disease drug resistance.Different cultivars composition carries out composite, is to prevent and treat the very common method of resistance disease.Heterogeneity is carried out composite, according to practical application effect, judge certain composite be synergy, add and or antagonism.In most cases, the composite effect of sterilization is all additive effect, really has the seldom composite of synergistic effect, especially synergistic effect clearly, very high composite just less of synergy ratio.Through inventor's research, find Gentrin Knotweed P.E and ZJ0712, azoles bacterium ester is composite after can produce good synergistic effect, and not yet open about the relevant report that Gentrin Knotweed P.E and ZJ0712, azoles bacterium ester are composite.
Summary of the invention
The object of the invention is to propose a kind ofly have that synergistic function, use cost are low, preventive effect is good containing the bactericidal composition of Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester.
The bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester that the present invention proposes contains active components A and active component B, active components A and active component B weight ratio are 1 ︰ 80 ~ 60 ︰ 1, described active components A is selected from Gentrin Knotweed P.E, active component B is selected from the one in ZJ0712, azoles bacterium ester, and active components A and the preferred weight ratio of active component B are 1 ︰ 60 ~ 40 ︰ 1; The weight ratio being more preferably Gentrin Knotweed P.E and ZJ0712 is 1 ︰ 25 ~ 20 ︰ 1, and the weight ratio of Gentrin Knotweed P.E and azoles bacterium ester is 1 ︰ 25 ~ 25 ︰ 1.
The bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester that the present invention proposes is for preventing and treating the purposes of disease on crops, and described crops comprise cereal crops, legume crop, fiber crop, sugar [yielding, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flower crop, medicinal crop, crop, green manure pasture crop; Described disease comprises powdery mildew, downy mildew, rust, anthracnose, gray mold, brown spot, scab or epidemic disease.
The weight ratio of active components A, active component B is 1 ︰ 80 ~ 60 ︰ 1.In usual composition, the weight percentage of active component is 0.5% ~ 90% of gross weight, is preferably 5% ~ 80%.According to different preparation types, active component content scope is different.Usually, liquid preparation contains the active substance of by weight 1% ~ 70%, is preferably 5% ~ 50%; Solid pharmaceutical preparation contains the active substance of by weight 5% ~ 80%, is preferably 10% ~ 80%.
At least containing a kind of surfactant in bactericidal composition of the present invention, the dispersion of active component in water when being beneficial to use.Surface-active contents is 5% ~ 30% of total formulation weight amount, and surplus is solid or liquid diluent.
Bactericidal composition of the present invention can by user before use through diluting or directly using.Its preparation can be prepared by common processing method known in those skilled in the art, after mixing with liquid flux or solid carrier by active substance, then add surfactant as one or more in dispersant, stabilizing agent, wetting agent, binding agent, defoamer, disintegrant, antifreeze etc.
Bactericidal composition of the present invention, acceptable formulation in any sterilization can be processed on demand, wherein more preferably formulation is wetting powder, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, microcapsule suspension-suspendinagent agent, can also make effervescent tablet, seed coat agent, ultra low volume liquids.
Composition comprises following constituent content when making wetting powder: active components A 1% ~ 60%, active component B1% ~ 80%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
Composition comprises following constituent content when making water dispersible granules: active components A 1% ~ 60%, active component B1% ~ 80%, dispersant 3% ~ 12%, wetting agent 1% ~ 8%, disintegrant 1% ~ 10%, binding agent 0 ~ 8%, filler surplus.
Composition comprises following constituent content when making suspending agent: active components A 0.5% ~ 50%, active component B0.5% ~ 50%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, defoamer 0.01% ~ 2%, thickener 0 ~ 2%, antifreeze 0 ~ 8%, deionized water add to 100%.
Composition comprises following constituent content when making suspension emulsion: active components A 0.5% ~ 50%, active component B0.5% ~ 50%, dispersant 2% ~ 10%, defoamer 0.01% ~ 2%, solvent 1% ~ 15%, thickener 0 ~ 2%, emulsifier 2% ~ 12%, antifreeze 0 ~ 8%, deionized water add to 100%.
Composition comprises following constituent content when making aqueous emulsion: active components A 0.5% ~ 50%, active component B0.5% ~ 50%, solvent 1% ~ 30%, emulsifier 1% ~ 15%, antifreeze 0 ~ 8%, thickener 0 ~ 2%, defoamer 0.01% ~ 2%, deionized water supply surplus.
Composition comprises following constituent content when making microemulsion: active components A 0.5% ~ 50%, active component B0.5% ~ 50%, solvent 1% ~ 30%, emulsifier 3% ~ 25%, antifreeze 0 ~ 8%, defoamer 0.01% ~ 2%, deionized water supply surplus.
Composition comprises following constituent content when making microcapsule suspending agent: active components A 0.5% ~ 50%, active component B0.5% ~ 50%, macromolecule cyst material 2% ~ 10%, dispersant 1% ~ 10%, organic solvent 1% ~ 10%, emulsifier 1% ~ 7%, defoamer 0.01% ~ 2%, pH adjusting agent 0.01% ~ 5%, deionized water add to 100%.
Composition comprises following constituent content when making microcapsule suspension-suspendinagent agent: active components A 0.5% ~ 50%, active component B0.5% ~ 50%, macromolecule cyst material 2% ~ 10%, dispersant 1% ~ 12%, wetting agent 1% ~ 8%, organic solvent 1% ~ 15%, emulsifier 1% ~ 6%, defoamer 0.01% ~ 2%, thickener 0 ~ 2%, pH adjusting agent 0.01% ~ 5%, deionized water add to 100%;
Wetting powder the key technical indexes of the present invention:
Suspending agent the key technical indexes of the present invention:
Aqueous emulsion the key technical indexes of the present invention:
Microemulsion the key technical indexes of the present invention:
Microcapsule suspending agent the key technical indexes of the present invention:
Microcapsule suspension-suspendinagent agent the key technical indexes of the present invention:
The invention has the advantages that:
(1) Gentrin Knotweed P.E and ZJ0712, azoles bacterium ester composite after, there is obvious synergy and lasting effect; (2) all greater activity is had to the disease on cereal crops, legume crop, fiber crop, sugar [yielding, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flower crop, medicinal crop, crop, green manure pasture crop; (3) decrease pesticide usage amount, reduce the residual quantity of agricultural chemicals on crop, alleviate environmental pollution; (4) to person poultry safety, Environmental compatibility is good; And preparation adhesion strength strengthens, resistance of rainwater washing against.
Embodiment
Below in conjunction with embodiment to further instruction of the present invention, the percentage in embodiment is all weight percentage, but the present invention is not limited thereto.
Application Example one
Embodiment 1 ~ 8 wetting powder
Gentrin Knotweed P.E, active component B, dispersant, wetting agent, filler are mixed in mixed cylinder, mixes again after airslide disintegrating mill is pulverized, wetting powder product of the present invention can be obtained, specifically in table 1.
The each component of table 1 embodiment 1 ~ 8 and weight portion
Embodiment 9 ~ 16 water dispersible granules
Gentrin Knotweed P.E, active component B, dispersant, wetting agent, disintegrant, filler etc. are obtained together the particle diameter needed through air-flow crushing, then add other auxiliary agents such as binding agent (can add and can not add), obtain granulation materials.Item is quantitatively sent in fluidized bed granulating dryer through granulating and after drying, water dispersible granules product of the present invention can being obtained, specifically in table 2.
The each component of table 2 embodiment 9 ~ 16 and weight portion
Embodiment 17 ~ 24 suspending agent
By dispersant, wetting agent, defoamer, thickener (can add and can not add), antifreeze (can add and can not add), mix through high speed shear, add Gentrin Knotweed P.E, active component B, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, surplus deionized water is supplied, and can obtain suspending agent product of the present invention, specifically in table 3.
The each component of table 3 embodiment 17 ~ 24 and weight portion
Embodiment 25 ~ 28 suspension emulsion
Dispersant, defoamer, thickener (can add and can not add), antifreeze (can add and can not add) are mixed through high speed shear, add active component B, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, obtained active component B suspending agent, then by Gentrin Knotweed P.E, solvent, emulsifier and the direct emulsification of various auxiliary agent high speed agitators in suspending agent, surplus deionized water is supplied, obtained suspension emulsion product of the present invention, specifically in table 4.
The each component of table 4 embodiment 25 ~ 28 and weight portion
Embodiment 29 ~ 34 aqueous emulsion
Gentrin Knotweed P.E, active component B, solvent, emulsifier are added together, make to be dissolved into homogeneous oil phase; Deionized water, antifreeze (can add and can not add), thickener (can add and can not add), defoamer are mixed, becomes homogeneous aqueous phase.Under high velocity agitation, aqueous phase is added oil phase, surplus deionized water is supplied; Aqueous emulsion product of the present invention can be obtained, specifically in table 5.
The each component of table 5 embodiment 29 ~ 34 and weight portion
By ZJ0712, azoles bacterium ester interchange in table 1 ~ 5, novel formulation can be obtained.
Embodiment 35 ~ 37 microemulsion
Gentrin Knotweed P.E, active component B, solvent, emulsifier, antifreeze (can add and can not add), defoamer are fully mixed into the oil phase of homogeneous transparent, under agitation slowly add deionized water, form WO emulsion, again through agitating heating, make it rapid phase inversion and become oil-in-water type, be chilled to room temperature to make it to reach balance, after filtration, surplus deionized water is supplied; Microemulsion product of the present invention can be obtained, specifically in table 6.
The each component of table 6 embodiment 35 ~ 37 and weight portion
Embodiment 38,39 microcapsule suspending agent
By Gentrin Knotweed P.E, active component B, macromolecule cyst material, solvent, make to be dissolved into homogeneous oil phase, in a shear condition, oil phase is joined in the aqueous phase solution containing emulsifier, pH adjusting agent, dispersant, surplus deionized water is supplied, bi-material reacts at oil-water interfaces, forms macromolecule cyst wall, makes the finely disseminated microcapsule suspending agent product of the present composition.Specifically in table 7.
Table 7 embodiment 38,39 each component and weight portion
Embodiment 40,41 microcapsule suspension-suspendinagent agent
By Gentrin Knotweed P.E, macromolecule cyst material, solvent, make to be dissolved into homogeneous oil phase, oil phase is joined in a shear condition in the aqueous phase solution containing emulsifier, pH adjusting agent, make finely disseminated microcapsule suspending agent.Dispersant, wetting agent, defoamer, thickener (can add and can not add) are mixed through high speed shear, add active component B, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, obtained suspending agent, then join in the aqueous phase solution of micro-capsule suspension by suspending agent, deionized water supplies surplus, make the finely disseminated microcapsule suspension-suspendinagent agent product of the present composition, specifically in table 8.
Table 8 embodiment 40,41 each component and weight portion
The embodiment of the present invention is the method adopting Toxicity Determination and field trial to combine.First pass through Toxicity Determination, synergy ratio (SR) after clear and definite two kinds of medicaments are composite by a certain percentage, SR < 0.5 is antagonism, 0.5≤SR≤1.5 are summation action, SR > 1.5 is synergistic effect, on this basis, then carry out field trial.
Test method: determine that after the effective inhibition concentration scope of each medicament, medicament establishes 5 dosage process respectively by active constituent content through prerun, if clear water contrast.Carry out with reference to " farm-chemical indoor determination test rule bactericide ", adopt mycelial growth rate method to measure medicament to the virulence of crop germ.Measure colony diameter by right-angled intersection method after 72h, calculate each process net growth, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) converts logarithm value (x) to, try to achieve virulence regression equation (y=a+bx) with method of least squares, and calculate the EC of often kind of medicament thus
50value.Calculate two medicament different ratio synergies ratio (SR) according to Wadley method, SR < 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action simultaneously, and SR > 1.5 is synergistic effect.Computing formula is as follows:
Wherein: a, b are respectively active components A and the ratio of active component B shared by combination;
A is Gentrin Knotweed P.E;
B is selected from the one in ZJ0712, azoles bacterium ester.
Application Example two:
For examination disease: powdery mildew of cucumber
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination Gentrin Knotweed P.E and the former medicine of ZJ0712 and the two different ratio mixture.
Toxicity test result
Table 9 Gentrin Knotweed P.E and the composite toxicity test analysis of results table to powdery mildew of cucumber of ZJ0712
As shown in Table 9, Gentrin Knotweed P.E and ZJ0712 proportioning are all greater than 1.5 when 1 ︰ 80 ~ 60 ︰ 1 to the synergy ratio SR of powdery mildew of cucumber, both explanations mixture in 1 ︰ 80 ~ 60 ︰ 1 scope all shows synergistic effect, when the proportioning of Gentrin Knotweed P.E and ZJ0712 is at 1 ︰ 25 ~ 20 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.25.Testing through applicant the optimum ratio finding Gentrin Knotweed P.E and ZJ0712 is 20:1,15:1,10:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:15,1:20,1:25, especially when Gentrin Knotweed P.E and ZJ0712 weight ratio are 1:2, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example three
For examination disease: downy mildew of garpe
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination Gentrin Knotweed P.E and the former medicine of azoles bacterium ester and the two different ratio mixture.
Table 10 Gentrin Knotweed P.E and the composite toxicity test analysis of results table to downy mildew of garpe of azoles bacterium ester
As shown in Table 10, Gentrin Knotweed P.E and azoles bacterium ester proportioning are all greater than 1.5 when 1 ︰ 80 ~ 60 ︰ 1 to the synergy ratio SR of downy mildew of garpe, both explanations mixture in 1 ︰ 80 ~ 60 ︰ 1 scope all shows synergistic effect, when the proportioning of Gentrin Knotweed P.E and azoles bacterium ester is at 1 ︰ 25 ~ 25 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.30.Testing through applicant the optimum ratio finding Gentrin Knotweed P.E and azoles bacterium ester is 25:1,20:1,15:1,10:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:15,1:20,1:25, especially when Gentrin Knotweed P.E and azoles bacterium ester weight ratio are 1:1, synergy ratio is maximum, and synergistic effect is the most obvious.
Through test find: Gentrin Knotweed P.E and active component B composite after have obvious synergistic effect to the control of the powdery mildew in various crop, downy mildew, rust, anthracnose, gray mold, brown spot, scab or epidemic disease, synergy ratio SR is all greater than 1.5.
Effect experiment part: test medicine is researched and developed by Shaanxi Meibang Pesticide Co., Ltd., provided, contrast medicament 10% Gentrin Knotweed P.E wetting powder (autogamy), 10% ZJ0712 suspending agent (autogamy), 20% azoles bacterium ester suspending agent (autogamy).
Application Example four Gentrin Knotweed P.E and active component B and compositely prevent and treat the powdery mildew of cucumber test of pesticide effectiveness
This test arrangement is at Xian District, Shanxi Province suburban area, and investigation powdery mildew of cucumber disease index before medicine, in the dispenser of disease initial stage of origination, 5 days, 15 days, 30 days investigation disease indexes after dispenser also calculate preventive effect.Result of the test is as follows:
Table 11 Gentrin Knotweed P.E and active component B and compositely prevent and treat the powdery mildew of cucumber test of pesticide effectiveness
As can be seen from Table 11, Gentrin Knotweed P.E and active component B composite after effectively can prevent and treat powdery mildew of cucumber, through applicant test find Gentrin Knotweed P.E and active component B composite after also can effectively prevent and treat cucumber downy mildew, epidemic disease, control efficiency is all higher than 97%, be better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example five Gentrin Knotweed P.E and active component B and the composite control watermelon anthrax test of pesticide effectiveness thereof
This test arrangement is in Weinan City Dali County, Shaanxi Province, and investigation watermelon anthrax disease index before medicine, in the dispenser of disease initial stage of origination, 3 days, 10 days, 20 days investigation disease indexes after dispenser also calculate preventive effect.Result of the test is as follows:
Table 12 Gentrin Knotweed P.E and active component B and the composite control watermelon anthrax test of pesticide effectiveness thereof
As can be seen from Table 12, Gentrin Knotweed P.E and active component B composite after effectively can prevent and treat watermelon anthrax, through applicant test find Gentrin Knotweed P.E and active component B composite after also can effectively prevent and treat watermelon powdery mildew, control efficiency is all higher than 97%, be better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example six Gentrin Knotweed P.E and active component B and compositely prevent and treat the wheat rust test of pesticide effectiveness
This test arrangement is in Weinan City suburb, Shaanxi Province, and investigation wheat rust disease index before medicine, in the dispenser of disease initial stage of origination, 5 days, 15 days, 30 days investigation disease indexes after dispenser also calculate preventive effect.Result of the test is as follows:
Table 13 Gentrin Knotweed P.E and active component B and compositely prevent and treat the wheat rust test of pesticide effectiveness
As can be seen from Table 13, Gentrin Knotweed P.E and active component B and compositely prevent and treat wheat rust, through applicant test find Gentrin Knotweed P.E and active component B composite after also can effectively prevent and treat wheat powdery mildew, brown spot, control efficiency is all higher than 96%, be better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
Application Example seven Gentrin Knotweed P.E and active component B and compositely prevent and treat the downy mildew of garpe test of pesticide effectiveness
This test arrangement is in Weinan City suburb, Shaanxi Province, and investigation downy mildew of garpe disease index before medicine, in the dispenser of disease initial stage of origination, 3 days, 10 days, 20 days investigation disease indexes after dispenser also calculate preventive effect.Result of the test is as follows:
Table 14 Gentrin Knotweed P.E and active component B and compositely prevent and treat the downy mildew of garpe test of pesticide effectiveness
As can be seen from Table 14, Gentrin Knotweed P.E and active component B composite after effectively can prevent and treat downy mildew of garpe, through applicant test find Gentrin Knotweed P.E and active component B composite after also can effectively prevent and treat grape powdery mildew, gray mold, control efficiency is all higher than 97%, be better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
After draw through tests different local throughout the country, Gentrin Knotweed P.E and ZJ0712, the composite rear preventive effect to Common Diseases such as the powdery mildew in various crop, downy mildew, rust, anthracnose, gray mold, brown spot, scab or epidemic diseases of azoles bacterium ester are all more than 95%, be better than single dose preventive effect, synergistic effect is obvious.
Claims (6)
1. one kind contains the bactericidal composition of Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester, effective active composition is active components A and active component B, it is characterized in that: active components A and active component B weight ratio are 1 ︰ 80 ~ 60 ︰ 1, described active components A is Gentrin Knotweed P.E, and active component B is selected from the one in ZJ0712, azoles bacterium ester.
2. the bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester according to claim 1, is characterized in that: the weight ratio of active components A and active component B is 1 ︰ 60 ~ 40 ︰ 1.
3. the bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester according to claim 2, is characterized in that:
The weight ratio of Gentrin Knotweed P.E and ZJ0712 is 1 ︰ 25 ~ 20 ︰ 1;
The weight ratio of Gentrin Knotweed P.E and azoles bacterium ester is 1 ︰ 25 ~ 25 ︰ 1.
4. the bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester according to any one of claim 1 to 3, is characterized in that: composition makes wetting powder, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent or microcapsule suspension-suspendinagent agent.
5. the bactericidal composition containing Gentrin Knotweed P.E and ZJ0712 or azoles bacterium ester according to claim 4 is for preventing and treating the purposes of disease on crops.
6. purposes according to claim 5, is characterized in that: described disease is powdery mildew, downy mildew, rust, anthracnose, gray mold, brown spot, scab or epidemic disease.
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Address after: 715500 Pucheng high tech Industrial Development Zone, Weinan, Shaanxi Applicant after: Shaanxi Mei Bang Pharmaceutical Group Limited by Share Ltd Address before: 715500 Shaanxi Pucheng Weinan County agricultural base industrial park Applicant before: Shaanxi Meibang Pesticide Co., Ltd. |
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| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160106 |