CN105203369B - Semi-automatic staining of sperm instrument - Google Patents
Semi-automatic staining of sperm instrument Download PDFInfo
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- CN105203369B CN105203369B CN201510687294.4A CN201510687294A CN105203369B CN 105203369 B CN105203369 B CN 105203369B CN 201510687294 A CN201510687294 A CN 201510687294A CN 105203369 B CN105203369 B CN 105203369B
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- sperm
- pedestal
- pressing plate
- long side
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Abstract
Semi-automatic staining of sperm instrument, including rectangular base, be arranged on pedestal along the arrangement of long side spacing parallel arranging some dyeing liquid containers and be arranged above pedestal and be padded device, the device that is padded includes the long side pressing plate horizontal with pedestal short side, some sperm coating glass pieces being arranged on pressing plate, long side both ends are hinged with sliding connecting device under pressing plate, pressing plate is spun upside down and can be reciprocatingly slided along pedestal long side.Using the method being padded and the special instrument controlling time is used, also solves the problem that coloring agent easily mixes, while has flushing and air-dry apparatus, the standardization of staining of sperm can be realized in fixed room temperature.Solve the problem of sperm morphological standards for dyeing, laid a good foundation for sperm morphological diagnostic standardization.
Description
Technical field
It is that rate of teratosperm checks equipment, more particularly to staining of sperm instrument the present invention relates to sperm morphological inspection.
Background technology
Sperm morphological inspection is rate of teratosperm inspection, and rate of teratosperm is larger to reproductive significance.Must be to essence before inspection
Son carries out special dyeing, and the colouring method used at present needs to use three kinds of reagents.Fixer, dyeing liquor A, dyeing liquor B. tri-
Kind reagent enters contact to the slide for being attached to sperm successively, needs to reduce the attached of former reagent as far as possible before using second of reagent
, but the object contact slide of water suction can not be used.There is standard the time of contact of each reagent, because during staining agent
Between length influence the effect of dyeing, and diagnosis of the Color to sperm morphological has a great influence.The effect of dyeing not only and
The length of staining agent time is also relevant about the purity levels with coloring agent and temperature.
The colouring method used at present has drop to contaminate, and contaminates.The method of drop dye refers to that three kinds of reagents are dropped on slide successively, this
Kind of method advantage will not be mixed between three kinds of reagents, and purity is guaranteed.Shortcoming be drop dye be it is pure by hand carry out, dyeing when
Between control nonstandard, for the more units of slide quantity, the various methods are very inconvenient, also compare waste coloring agent, pollute environment.
Dip-dye is slide to be immersed successively in three kinds of reagent tanks, departure work and automatic.Advantage is that immerged time control is stricter, particularly
The instrument of automation is more preferable to time control.Contaminate disadvantage and be that former reagent can bring latter reagent into, reagent
Purity is difficult to control, because purity cannot be guaranteed that Color is also relatively difficult to ensure card after group reagent use for several times.Often change examination
Agent is larger waste, and the dip-dye equipment instrument of automation is larger, and price is higher.Therefore the standardization of staining of sperm is compared at present
It is difficult.
The content of the invention
It is easy to operate to have by way of being padded in view of the deficiencies of the prior art, the present invention provides a kind of simple in construction
Effect prevents coloured differently agent from mixing, and improves dyeing quality, realizes the semi-automatic staining of sperm instrument of staining of sperm standardization.
To realize the object of the invention, there is provided following technical scheme：Semi-automatic staining of sperm instrument, it is characterised in that including length
Square base, it is arranged at and dyes liquid containers along some of long side spacing parallel arranging arrangement on pedestal and be arranged above pedestal
It is padded device, some sperms painting for being padded device and including the long side pressing plate horizontal with pedestal short side, being arranged on pressing plate
Sheet glass is covered, long side both ends are hinged with sliding connecting device under pressing plate, pressing plate is spun upside down and can be reciprocal along pedestal long side
Slide.
If preferably, dyeing liquid container includes the strip matrix affixed with pedestal and is arranged on the matrix of strip
Dry bowl-shape liquid storage container, bowl-shape liquid storage container is plastic elastomeric.
Preferably, sperm coating glass piece and bowl-shape liquid storage container position are mutually corresponding.
Pedestal long side is provided with guide groove, the slip on the pedestal at liquid container upper and lower ends preferably, dyeing
Attachment means inlay card is in guide groove.
Preferably, long side is provided with depression bar on pressing plate, depression bar is provided at both ends with handle.
Preferably, air outlet is offered on depression bar corresponds to sperm coating glass piece on pressing plate with delivery port respectively, go out
Air port is connected air-supply arrangement and water supply installation with delivery port by pipeline.
Preferably, being provided with time prompting device on the pedestal, it is provided with depression bar and is used cooperatively with time prompting device
Timing button.
Beneficial effects of the present invention：The present invention is using the method being padded and uses the special instrument controlling time, also solves
The problem that coloring agent easily mixes, while have flushing and air-dry apparatus, the mark of staining of sperm can be realized in fixed room temperature
Standardization.Solve the problem of sperm morphological standards for dyeing, laid a good foundation for sperm morphological diagnostic standardization.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Embodiment
Embodiment 1:Semi-automatic staining of sperm instrument, including rectangular base 1, be arranged on pedestal 1 along long side spacing parallel arranging
3 of arrangement, which dye liquid containers 2 and are arranged above pedestal 1, is padded device 3, and the device 3 that is padded includes long side and base
The horizontal pressing plate 3.1 of 1 short side of seat, 3 sperm coating glass piece 3.1.1 being arranged on pressing plate 3.1,3.1 times long sides of pressing plate two
End is hinged with sliding connecting device 4, pressing plate 3.1 is spun upside down and can be reciprocatingly slided along the long side of pedestal 1.Dye liquid container 2
Including the strip matrix 2.1 affixed with pedestal 1 and 3 bowl-shape liquid storage containers 2.2 being arranged on the matrix 2.1 of strip, bowl
Shape liquid storage container 2.2 is plastic elastomeric.Sperm coating glass piece 3.1.1 and the bowl-shape position of liquid storage container 2.2 are mutually corresponding.Dye
The long side of pedestal 1 is provided with guide groove 5 on pedestal 1 at the upper and lower ends of color liquid container 2, the inlay card of sliding connecting device 4 in
In guide groove 5, it is slidably connected with guide groove 5.Long side is provided with depression bar 6 on pressing plate 3.1, and depression bar 6 is provided at both ends with handle 6.1.
Air outlet 6.2 is offered on depression bar 6 and corresponds to sperm coating glass piece 3.1.1 on pressing plate 3.1, air-out respectively with delivery port 6.3
Mouth 6.2 is connected air-supply arrangement and water supply installation with delivery port 6.3 by pipeline.Time prompting device 7 is provided with the pedestal 1,
The timing button 8 being used cooperatively with time prompting device 7 is provided with depression bar 6.The sliding connecting device 4 is normal in the prior art
Connecting rod, auricle and articulated elements, particularly as not described.
Claims (7)
1. semi-automatic staining of sperm instrument, it is characterised in that including rectangular base, be arranged on pedestal along long side spacing parallel arranging and arrange
Some of cloth dyeing liquid containers and the device that is padded being arranged above pedestal, it is described to be padded that device includes long side and pedestal is short
The horizontal pressing plate in side, some sperm coating glass pieces being arranged on pressing plate, long side both ends, which are hinged with, under pressing plate is slidably connected
Device, pressing plate is set to spin upside down and can be reciprocatingly slided along pedestal long side.
2. semi-automatic staining of sperm instrument according to claim 1, it is characterised in that dyeing liquid container includes affixed with pedestal
Strip matrix and several bowl-shape liquid storage containers for being arranged on the matrix of strip, bowl-shape liquid storage container is plastic resilient
Body.
3. semi-automatic staining of sperm instrument according to claim 1, it is characterised in that sperm coating glass piece and bowl-shape liquid storage
Container position is mutually corresponding.
4. semi-automatic staining of sperm instrument according to claim 1, it is characterised in that the base at dyeing liquid container upper and lower ends
Pedestal long side is provided with guide groove on seat, the sliding connecting device inlay card is in guide groove.
5. semi-automatic staining of sperm instrument according to claim 1, it is characterised in that long side is provided with depression bar, depression bar on pressing plate
It is provided at both ends with handle.
6. semi-automatic staining of sperm instrument according to claim 5, it is characterised in that air outlet and water outlet are offered on depression bar
Mouth corresponds to the sperm coating glass piece on pressing plate respectively, and air outlet is connected air-supply arrangement and supplied water and filled with delivery port by pipeline
Put.
7. semi-automatic staining of sperm instrument according to claim 5, it is characterised in that be provided with time prompting on the pedestal
Device, the timing button being used cooperatively with time prompting device is provided with depression bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510687294.4A CN105203369B (en) | 2015-10-22 | 2015-10-22 | Semi-automatic staining of sperm instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510687294.4A CN105203369B (en) | 2015-10-22 | 2015-10-22 | Semi-automatic staining of sperm instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105203369A CN105203369A (en) | 2015-12-30 |
| CN105203369B true CN105203369B (en) | 2018-02-13 |
Family
ID=54951167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510687294.4A Active CN105203369B (en) | 2015-10-22 | 2015-10-22 | Semi-automatic staining of sperm instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105203369B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2439510A1 (en) * | 2010-10-06 | 2012-04-11 | Klinipath B.V. | Method and device for preparing tissue and mould for pretreating tissue material |
| CN102830003A (en) * | 2012-08-28 | 2012-12-19 | 浙江世纪康大医疗科技有限公司 | Horizontal-pressurizing type dyeing cover plate |
| WO2014066950A1 (en) * | 2012-11-01 | 2014-05-08 | Leica Biosystems Melbourne Pty Ltd | Slide staining assembly and cover member |
| US8900857B2 (en) * | 2008-06-18 | 2014-12-02 | Leica Biosystems Melbourne Pty Ltd | Devices and methods for tissue handling and embedding |
| CN204422859U (en) * | 2015-03-13 | 2015-06-24 | 遵义医学院 | A kind of Novel sperm dyeing glass slide and cover glass |
| CN204495638U (en) * | 2015-02-13 | 2015-07-22 | 中南大学 | A kind of Chromosome specimen dropping device |
| CN205120470U (en) * | 2015-10-22 | 2016-03-30 | 宜兴市妇幼保健所 | Semi -automatic sperm dyeing instrument |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9121798B2 (en) * | 2013-08-26 | 2015-09-01 | Rarecyte, Inc. | Apparatus for spreading a fluid across a substrate and method of using the same |
-
2015
- 2015-10-22 CN CN201510687294.4A patent/CN105203369B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8900857B2 (en) * | 2008-06-18 | 2014-12-02 | Leica Biosystems Melbourne Pty Ltd | Devices and methods for tissue handling and embedding |
| EP2439510A1 (en) * | 2010-10-06 | 2012-04-11 | Klinipath B.V. | Method and device for preparing tissue and mould for pretreating tissue material |
| CN102830003A (en) * | 2012-08-28 | 2012-12-19 | 浙江世纪康大医疗科技有限公司 | Horizontal-pressurizing type dyeing cover plate |
| WO2014066950A1 (en) * | 2012-11-01 | 2014-05-08 | Leica Biosystems Melbourne Pty Ltd | Slide staining assembly and cover member |
| CN204495638U (en) * | 2015-02-13 | 2015-07-22 | 中南大学 | A kind of Chromosome specimen dropping device |
| CN204422859U (en) * | 2015-03-13 | 2015-06-24 | 遵义医学院 | A kind of Novel sperm dyeing glass slide and cover glass |
| CN205120470U (en) * | 2015-10-22 | 2016-03-30 | 宜兴市妇幼保健所 | Semi -automatic sperm dyeing instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105203369A (en) | 2015-12-30 |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ding Xufeng Inventor after: Cai Yijuan Inventor after: Ma Tieliang Inventor after: Hu Yuqing Inventor after: Wang Honghua Inventor after: Shi Qingjing Inventor after: Zheng Huaping Inventor before: Ding Xufeng Inventor before: Cai Yijuan Inventor before: Hu Yuqing Inventor before: Wang Honghua Inventor before: Zheng Huaping Inventor before: Shi Qingjing |
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| CB03 | Change of inventor or designer information |