CN105203369A - Semi-automatic sperm stainer - Google Patents
Semi-automatic sperm stainer Download PDFInfo
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- CN105203369A CN105203369A CN201510687294.4A CN201510687294A CN105203369A CN 105203369 A CN105203369 A CN 105203369A CN 201510687294 A CN201510687294 A CN 201510687294A CN 105203369 A CN105203369 A CN 105203369A
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- sperm
- pressing plate
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- pedestal
- staining
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Abstract
The invention relates to a semi-automatic sperm stainer. The semi-automatic sperm stainer comprises a rectangular base, a plurality of staining solution containers which are arranged on the base and are arranged in parallel at an interval along a long side, and a press-staining device which is arranged above the base, wherein the press-staining device comprises a pressing plate with long sides which are horizontal with short sides of the base and a plurality of sperm coating glass sheets which are arranged on the pressing plate, and the two ends of the lower long side of the pressing plate are hinged with slidable connecting devices so that the pressing plate can turn over up and down and can slide back and forth along the long sides of the base. By using a press-staining method and using a special instrument to control time, the problem that staining agents are easily mixed is solved; since washing and air-drying devices are provided, the standardization of sperm staining can be realized at fixed room temperature. The problem of sperm morphology staining standardization is solved and a foundation is laid for sperm morphology diagnosis standardization.
Description
Technical field
The present invention relates to sperm morphological inspection and rate of teratosperm checkout facility, particularly relate to staining of sperm instrument.
Background technology
Sperm morphological inspection and rate of teratosperm inspection, rate of teratosperm is larger to reproductive significance.Must carry out special dyeing to sperm before inspection, the colouring method used at present needs use three kinds of reagent.Immobile liquid, dyeing liquor A, dyeing liquor B. tri-kinds of reagent enter contact to the slide that attached to sperm successively, need the attachment of a kind of reagent before as far as possible reducing before use the second reagent, but can not with the object contact slide absorbed water.There is standard the duration of contact of each reagent, because the effect of the length impact dyeing of staining agent time, and the diagnosis impact of Color on sperm morphological is larger.The effect of dyeing not only purity levels with coloring agent relevant to the length of staining agent time and temperature also relevant.
The colouring method of current use has a dye, contaminates.The method of dripping dye refers to that three kinds of reagent drop on slide successively, and this method advantage can not mix between three kinds of reagent, and purity is guaranteed.Shortcoming is a dye is that pure craft is carried out, and the time controling of dyeing is nonstandard, very inconvenient for this various method of the many units of slide quantity, also compares waste coloring agent, contaminated environment.Dip-dye is that slide is immersed in three kinds of reagent tanks successively, divides manual and automatic.Advantage is that immerged time controls comparatively strictly, and particularly the instrument of robotization is better to time controling.Contaminate disadvantage and be that front a kind of reagent can bring rear a kind of reagent into, the purity of reagent is wayward, because purity can not ensure that Color is also comparatively difficult to ensure card after a group reagent uses for several times.Frequent replacing reagent is larger waste, and the dip-dye instrument volume of robotization is comparatively large, and price is higher.Therefore the standardization of staining of sperm is more difficult at present.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, provide a kind of structure simple, the easy to operate mode by pressure dye effectively prevents coloured differently agent from mixing, and improves dyeing quality, realizes the standardized semi-automatic staining of sperm instrument of staining of sperm.
For realizing the object of the invention, provide following technical scheme: semi-automatic staining of sperm instrument, it is characterized in that comprising rectangular base, be arranged on pedestal along long limit spacing parallel arranging arrangement some dyeing liquor containers and be arranged at above pedestal pressure dye device, described pressure dye device comprises the pressing plate of long limit and pedestal minor face level, some the sperm coated glass sheets be arranged on pressing plate, under pressing plate, two ends, long limit are hinged with sliding connecting device, pressing plate can be spun upside down and can reciprocatingly slide along the long limit of pedestal.
As preferably, dyeing liquor container comprises the strip matrix affixed with pedestal and is arranged at several the bowl-shape liquid storage containers on the matrix of strip, and bowl-shape liquid storage container is plastic elastomeric.
As preferably, sperm coated glass sheet is mutually corresponding with bowl-shape liquid storage container position.
As preferably, the pedestal at dyeing liquor container upper and lower two ends place is provided with guide-track groove along the long limit of pedestal, and described sliding connecting device inlay card is in guide-track groove.
As preferably, on pressing plate, long limit is provided with depression bar, and depression bar two ends are provided with handle.
As preferably, depression bar offers air outlet and the water delivering orifice sperm coated glass sheet respectively on corresponding pressing plate, air outlet is connected air-supply arrangement and water supply installation with water delivering orifice by pipeline.
As preferably, described pedestal is provided with time prompting device, depression bar is provided with time prompting device with the use of timing button.
Beneficial effect of the present invention: the present invention uses the method for pressure dye and uses the special instrument controlling time, also solves the difficult problem that coloring agent easily mixes, and has flushing and air-dry apparatus simultaneously, can realize the standardization of staining of sperm in fixing room temperature.Solve a difficult problem for sperm morphological standards for dyeing, for sperm morphological diagnostic standardization is laid a good foundation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
Embodiment 1: semi-automatic staining of sperm instrument, comprise rectangular base 1, be arranged on pedestal 1 along long limit spacing parallel arranging arrangement 3 dyeing liquor containers 2 and be arranged at above pedestal 1 pressure dye device 3, described pressure dye device 3 comprises the pressing plate 3.1 of long limit and pedestal 1 minor face level, 3 the sperm coated glass sheet 3.1.1 be arranged on pressing plate 3.1, two ends, pressing plate 3.1 times long limits are hinged with sliding connecting device 4, pressing plate 3.1 can be spun upside down and can reciprocatingly slide along the long limit of pedestal 1.Dyeing liquor container 2 comprises the strip matrix 2.1 affixed with pedestal 1 and is arranged at 3 bowl-shape liquid storage containers 2.2 on the matrix 2.1 of strip, and bowl-shape liquid storage container 2.2 is plastic elastomeric.Sperm coated glass sheet 3.1.1 is mutually corresponding with bowl-shape liquid storage container 2.2 position.The pedestal 1 at dyeing liquor container about 2 two ends place is provided with guide-track groove 5 along the long limit of pedestal 1, and described sliding connecting device 4 inlay card, in guide-track groove 5, is slidably connected with guide-track groove 5.On pressing plate 3.1, long limit is provided with depression bar 6, and depression bar 6 two ends are provided with handle 6.1.Depression bar 6 offers air outlet 6.2 and the sperm coated glass sheet 3.1.1 on water delivering orifice 6.3 respectively corresponding pressing plate 3.1, air outlet 6.2 is connected air-supply arrangement and water supply installation with water delivering orifice 6.3 by pipeline.Described pedestal 1 is provided with time prompting device 7, depression bar 6 is provided with time prompting device 7 with the use of timing button 8.Described sliding connecting device 4 is connecting link, auricle and articulated elements conventional in prior art, is not specifically just described.
Claims (7)
1. semi-automatic staining of sperm instrument, it is characterized in that comprising rectangular base, be arranged on pedestal along long limit spacing parallel arranging arrangement some dyeing liquor containers and be arranged at above pedestal pressure dye device, described pressure dye device comprises the pressing plate of long limit and pedestal minor face level, some the sperm coated glass sheets be arranged on pressing plate, under pressing plate, two ends, long limit are hinged with sliding connecting device, pressing plate can be spun upside down and can reciprocatingly slide along the long limit of pedestal.
2. semi-automatic staining of sperm instrument according to claim 1, it is characterized in that dyeing liquor container comprises the strip matrix affixed with pedestal and is arranged at several the bowl-shape liquid storage containers on the matrix of strip, bowl-shape liquid storage container is plastic elastomeric.
3. semi-automatic staining of sperm instrument according to claim 1, is characterized in that sperm coated glass sheet is mutually corresponding with bowl-shape liquid storage container position.
4. semi-automatic staining of sperm instrument according to claim 1, the pedestal that it is characterized in that dyeing liquor container upper and lower two ends place is provided with guide-track groove along the long limit of pedestal, and described sliding connecting device inlay card is in guide-track groove.
5. semi-automatic staining of sperm instrument according to claim 1, it is characterized in that on pressing plate, long limit is provided with depression bar, depression bar two ends are provided with handle.
6. semi-automatic staining of sperm instrument according to claim 5, it is characterized in that depression bar offering air outlet and the sperm coated glass sheet on water delivering orifice respectively corresponding pressing plate, air outlet is connected air-supply arrangement and water supply installation with water delivering orifice by pipeline.
7. semi-automatic staining of sperm instrument according to claim 5, is characterized in that described pedestal is provided with time prompting device, depression bar is provided with time prompting device with the use of timing button.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510687294.4A CN105203369B (en) | 2015-10-22 | 2015-10-22 | Semi-automatic staining of sperm instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510687294.4A CN105203369B (en) | 2015-10-22 | 2015-10-22 | Semi-automatic staining of sperm instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105203369A true CN105203369A (en) | 2015-12-30 |
| CN105203369B CN105203369B (en) | 2018-02-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510687294.4A Active CN105203369B (en) | 2015-10-22 | 2015-10-22 | Semi-automatic staining of sperm instrument |
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| CN (1) | CN105203369B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2439510A1 (en) * | 2010-10-06 | 2012-04-11 | Klinipath B.V. | Method and device for preparing tissue and mould for pretreating tissue material |
| CN102830003A (en) * | 2012-08-28 | 2012-12-19 | 浙江世纪康大医疗科技有限公司 | Horizontal-pressurizing type dyeing cover plate |
| WO2014066950A1 (en) * | 2012-11-01 | 2014-05-08 | Leica Biosystems Melbourne Pty Ltd | Slide staining assembly and cover member |
| US8900857B2 (en) * | 2008-06-18 | 2014-12-02 | Leica Biosystems Melbourne Pty Ltd | Devices and methods for tissue handling and embedding |
| US20150056695A1 (en) * | 2013-08-26 | 2015-02-26 | Rarecyte, Inc. | Apparatus for spreading a fluid across a substrate and method of using the same |
| CN204422859U (en) * | 2015-03-13 | 2015-06-24 | 遵义医学院 | A kind of Novel sperm dyeing glass slide and cover glass |
| CN204495638U (en) * | 2015-02-13 | 2015-07-22 | 中南大学 | A kind of Chromosome specimen dropping device |
| CN205120470U (en) * | 2015-10-22 | 2016-03-30 | 宜兴市妇幼保健所 | Semi -automatic sperm dyeing instrument |
-
2015
- 2015-10-22 CN CN201510687294.4A patent/CN105203369B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8900857B2 (en) * | 2008-06-18 | 2014-12-02 | Leica Biosystems Melbourne Pty Ltd | Devices and methods for tissue handling and embedding |
| EP2439510A1 (en) * | 2010-10-06 | 2012-04-11 | Klinipath B.V. | Method and device for preparing tissue and mould for pretreating tissue material |
| CN102830003A (en) * | 2012-08-28 | 2012-12-19 | 浙江世纪康大医疗科技有限公司 | Horizontal-pressurizing type dyeing cover plate |
| WO2014066950A1 (en) * | 2012-11-01 | 2014-05-08 | Leica Biosystems Melbourne Pty Ltd | Slide staining assembly and cover member |
| US20150056695A1 (en) * | 2013-08-26 | 2015-02-26 | Rarecyte, Inc. | Apparatus for spreading a fluid across a substrate and method of using the same |
| CN204495638U (en) * | 2015-02-13 | 2015-07-22 | 中南大学 | A kind of Chromosome specimen dropping device |
| CN204422859U (en) * | 2015-03-13 | 2015-06-24 | 遵义医学院 | A kind of Novel sperm dyeing glass slide and cover glass |
| CN205120470U (en) * | 2015-10-22 | 2016-03-30 | 宜兴市妇幼保健所 | Semi -automatic sperm dyeing instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105203369B (en) | 2018-02-13 |
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Inventor after: Ding Xufeng Inventor after: Cai Yijuan Inventor after: Ma Tieliang Inventor after: Hu Yuqing Inventor after: Wang Honghua Inventor after: Shi Qingjing Inventor after: Zheng Huaping Inventor before: Ding Xufeng Inventor before: Cai Yijuan Inventor before: Hu Yuqing Inventor before: Wang Honghua Inventor before: Zheng Huaping Inventor before: Shi Qingjing |