CN105200799A - Technology for microorganism modification of silk broadcloth - Google Patents
Technology for microorganism modification of silk broadcloth Download PDFInfo
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- CN105200799A CN105200799A CN201510593070.7A CN201510593070A CN105200799A CN 105200799 A CN105200799 A CN 105200799A CN 201510593070 A CN201510593070 A CN 201510593070A CN 105200799 A CN105200799 A CN 105200799A
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Abstract
The invention discloses a method for microorganism modification of silk broadcloth. The method comprises the steps of composite bactericide preparing, wherein lactobacillus casei and sphingomonas are prepared into a composite bactericide; fermentation culture solution preparing, wherein a fermentation culture solution is prepared from corn steep liquor, trehalose, chitosan and vitamin H, and the pH value of the solution is 7.0-7.5; sterilizing and then cooling; fermenting, wherein a silk fabric is put into the fermentation culture solution, and the bactericide is inoculated for fermentation; inactivation treating, wherein the fabric is taken out, put in water with the temperature of 80 DEG C for 15 min-30 min and then rinsed up with clean water; low-temperature drying, wherein drying is performed at the low temperature of 60 DEG C. According to the method, the technology is simple, easy to operate and good in modification effect, the antibacterial property and the wrinkle resistance of the silk fabric are greatly enhanced and cannot be significantly reduced along with washing, and the silk fabric has the good washing resistance.
Description
Technical field
The present invention relates to the technique of a kind of silk broadcloth microorganism modification, belong to material treatment field.
Background technology
The real silk fabric wearability that to have that gloss is soft, feel is soft and smooth, moisture absorption is breathed freely etc. excellent, is particularly suitable for making the products such as the underwear that has higher requirements to antibiotic property and infant clothing.But the shortcomings such as real silk fabric creases easily, easily shrink, difficulty is arranged, have impact on its result of use.In order to overcome the deficiency of real silk fabric, for many years, scientific and technical personnel constantly explore the technological approaches improving silk broadcloth wearability, wherein adopt finishing agent to be one of main research direction of raising real silk fabric antibacterial crease-resistant performance both at home and abroad at present.
It is there is not chemical crosslinking between Harmonic Protein Molecular Chains due to cocoon fiber that silk broadcloth creases easily, only there is hydrogen bond, the bonding action of ionic bond etc., and intermolecular amorphous region accounts for 50%, when these amorphous regions are by hydrone or other solvent actions, these keys are easily damaged, weaken the active force between large molecule, fiber is made easily Relative sliding and distortion to occur between strand under external force, after hydrone or other solvent actions remove, there is no enough restraining forces between macromolecular chain and make it get back to original position, the change of silk fiber is an irreversible process, show in silk fabrics be exactly shrink and wrinkling.
Prior art by finishing agent, comprises the biological novel finishing agents such as shitosan to improve the antibiotic property of real silk fabric, wrinkle resistance usually.But arrange silk broadcloth after simulation washing, antibiotic property, dry wet elasticity decline all to some extent.
Using microorganism to improve fabric is a brand-new field, and fermentable cost is low, is the following new technique having very much market potential.
The object of this invention is to provide a kind of technique of new silk broadcloth microorganism modification, by specific microorganism and fermentation mode, reach the antibiotic property improving real silk fabric, the object of wrinkle resistance, and these performances can not disappear along with the increased frequency of washing.Through retrieval, such technical scheme is not also disclosed.
Summary of the invention
Based on the technical problem that background technology exists, the present invention is directed to background technology Problems existing, the technique of one one kinds of new silk broadcloth microorganism modifications is provided, by specific microorganism and fermentation mode, reach the antibiotic property improving real silk fabric, the object of wrinkle resistance, and these performances can not disappear along with the increased frequency of washing.
Object of the present invention is achieved through the following technical solutions:
The method of a kind of silk broadcloth microorganism modification:
(1) prepare composite bacteria agent capable: by Lactobacillus casei, Sphingol single-cell is mixed with composite bacteria agent capable;
(2) fermentation culture is configured: corn steep liquor, trehalose, shitosan, biotin, pH:7.0-7.5; Cool after sterilizing;
(3) ferment: silk fabric is dropped in fermentation culture, access bacteria fermentation;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 15min-30min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
As preferably, step (1) described Lactobacillus casei, Sphingol single-cell mixes according to weight ratio 2-3:1-1.5.
As preferably, step (2) is: corn steep liquor 3-6g/L, trehalose 2-3g/L, shitosan 2-3g/L, biotin: 20ug/L, pH:7.0-7.5; 33 degree are cooled to after sterilizing.
As preferably, step (3) is: dropped into by silk fabric in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 37-39 DEG C, incubation time 5-15 hour.
As preferably, described silk fabric is 02 crepe de Chine real silk fabric.
Sphingol single-cell in the environment distributed pole is extensive, and has very strong patience (as all once found the existence of Sphingol single-cell in the extreme environment such as polar region, intense radiation) to adverse circumstances.The degraded substrate spectrum of Sphingol single-cell is wide, and the degraded as the aromatics such as Dui dioxin, phenols, azo dyes and xenobiotic condensate etc. has relevant report.Because Sphingol single-cell has metabolic capability very widely to aromatic, and some bacterial classification of this Pseudomonas can synthesize the outer biopolymer of valuable born of the same parents, and therefore, Sphingol single-cell becomes the focus being concerned and studying in recent years.
The Nature has abundant microbial resources, and just select different types of microorganisms for production technologies such as food, the modern times more have for medical treatment, even sewage disposal from ancient times, but study on the modification microorganism being used for fabric is also considerably less.The present inventor is by hardships for many years work, and find that Sphingol single-cell and Lactobacillus casei can with the use of for Modification of Silk Fabrics, such technology does not also have report.
In addition, the sweat of microorganism is ever-changing, and conservative control zymotechnique is also very important.
Usefulness of the present invention is:
The simply good operation of present invention process, modified effect is good, greatly strengthen antibiotic property and the wrinkle resistance of real silk fabric, and does not significantly reduce along with washing, very water-fastness.
Detailed description of the invention
embodiment 1:
Prepare composite bacteria agent capable:
Lactobacillus casei and Sphingol single-cell are purchase and obtain, and many patent documents also have open.
(1) Lactobacillus casei (Lactobacillus) is prepared:
Slant medium (g/L): 20 glucose, 5 dusty yeasts, 10 soy peptones, 10 beef extracts, 5NaCl, 10 sodium acetates, 2 ammonium citrates, 0.2MgSO47H2O, 0.05MnSO47H2O, 15 agar, pH6.5.
Fermentation medium (g/L): 40 glucose, 10 dusty yeasts, 10 soy peptones, inorganic salts (0.01NaCl, 0.5 sodium acetate, 0.2 ammonium citrate, 0.2KH2PO4,0.2MgSO47H2O, 0.05MnSO47H2O), pH6.5.
Fermentation process: get 1 ring and be inoculated in 500mL shaking flask on inclined-plane, liquid amount is 150mL, at 37 DEG C, 150rpm cultivates 24h to logarithmic phase.The centrifugal 10min of 5000rpm collects thalline.
(2) prepare Sphingol single-cell (
sphingomonassp
.):
Slant medium (g/L): 20 glucose, 5 dusty yeasts, 10 soy peptones, 10 beef extracts, 5NaCl, 10 sodium acetates, 2 ammonium citrates, 0.2MgSO47H2O, 0.05MnSO47H2O, 15 agar, pH7.0.
Fermentation medium (g/L): 40 glucose, 10 dusty yeasts, 10 soy peptones, inorganic salts (0.01NaCl, 0.5 sodium acetate, 0.2 ammonium citrate, 0.2KH2PO4,0.2MgSO47H2O, 0.05MnSO47H2O), pH6.5.
Fermentation process: get 1 ring and be inoculated in 500mL shaking flask on inclined-plane, liquid amount is 150mL, at 37 DEG C, 150rpm cultivates 24h to logarithmic phase.The centrifugal 10min of 5000rpm collects thalline.
(3) candida utili (Candidautilis) is prepared:
Slant medium: 10 ~ 12 ° of Brix brewer's worts, 15g/L agar, pH nature.
Fermentation medium: 10 ~ 12 ° of Brix brewer's worts, pH nature.
Fermentation process: get 1 ring and be inoculated in 500mL shaking flask on inclined-plane, liquid amount is 150mL, under the optimum temperature of individual different bacterium, 150rpm is cultured to logarithmic phase.The centrifugal 10min of 5000rpm collects thalline.
Be that 2-3:1-1.5 is hybridly prepared into composite bacteria agent capable by the Lactobacillus casei prepared, Sphingol single-cell according to weight ratio.
Candida utili tests use in contrast.
embodiment 2:
The method of silk broadcloth microorganism modification:
(1) composite bacteria agent capable is prepared: according to the method for embodiment 1 by Lactobacillus casei, Sphingol single-cell is mixed with composite bacteria agent capable, described Lactobacillus casei, and Sphingol single-cell mixes according to weight ratio 2:1.5;
(2) fermentation culture is configured: corn steep liquor 3g/L, trehalose 3g/L, shitosan 2g/L, biotin: 20ug/L, pH:7.5; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 37 DEG C, incubation time 15 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 15min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 3:
The method of silk broadcloth microorganism modification:
(1) composite bacteria agent capable is prepared: according to the method for embodiment 1 by Lactobacillus casei, Sphingol single-cell is mixed with composite bacteria agent capable, described Lactobacillus casei, and Sphingol single-cell mixes according to weight ratio 3:1;
(2) fermentation culture is configured: corn steep liquor 6g/L, trehalose 2g/L, shitosan 3g/L, biotin: 20ug/L, pH:7.0; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 39 DEG C, incubation time 5 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 30min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 4:
The method of silk broadcloth microorganism modification:
(1) composite bacteria agent capable is prepared: according to the method for embodiment 1 by Lactobacillus casei, Sphingol single-cell is mixed with composite bacteria agent capable, described Lactobacillus casei, and Sphingol single-cell mixes according to weight ratio 2:1.3;
(2) fermentation culture is configured: corn steep liquor 4g/L, trehalose 3g/L, shitosan 2g/L, biotin: 20ug/L, pH:7.4; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 38 DEG C, incubation time 10 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 20min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 5:
The method of silk broadcloth microorganism modification:
(1) composite bacteria agent capable is prepared: according to the method for embodiment 1 by Lactobacillus casei, Sphingol single-cell is mixed with composite bacteria agent capable, described Lactobacillus casei, and Sphingol single-cell mixes according to weight ratio 3:1.1;
(2) fermentation culture is configured: corn steep liquor 5g/L, trehalose 2g/L, shitosan 3g/L, biotin: 20ug/L, pH:7.1; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 37 DEG C, incubation time 8 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 23min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 6:
Only with Lactobacillus casei:
The method of silk broadcloth microorganism modification:
(1) microbial inoculum is prepared: Lactobacillus casei is mixed with microbial inoculum by the method according to embodiment 1;
(2) fermentation culture is configured: corn steep liquor 3g/L, trehalose 2g/L, shitosan 3g/L, biotin: 20ug/L, pH:7.3; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 39 DEG C, incubation time 13 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 16min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 7:
Only with Sphingol single-cell:
The method of silk broadcloth microorganism modification:
(1) microbial inoculum is prepared: Sphingol single-cell is mixed with microbial inoculum by the method according to embodiment 1;
(2) fermentation culture is configured: corn steep liquor 6g/L, trehalose 3g/L, shitosan 2g/L, biotin: 20ug/L, pH:7.2; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 38 DEG C, incubation time 6 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 29min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 8:
Only with candida utili:
The method of silk broadcloth microorganism modification:
(1) microbial inoculum is prepared: candida utili is mixed with microbial inoculum by the method according to embodiment 1;
(2) fermentation culture is configured: corn steep liquor 5g/L, trehalose 2g/L, shitosan 3g/L, biotin: 20ug/L, pH:7.5; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 37 DEG C, incubation time 14 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 19min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 9:
The control of fermentation condition:
The method of silk broadcloth microorganism modification:
(1) composite bacteria agent capable is prepared: according to the method for embodiment 1 by Lactobacillus casei, Sphingol single-cell is mixed with composite bacteria agent capable, described Lactobacillus casei, and Sphingol single-cell mixes according to weight ratio 3:1;
(2) fermentation culture is configured: corn steep liquor 4g/L, biotin: 20ug/L, pH:7.0; 33 degree are cooled to after sterilizing;
(3) ferment: 02 crepe de Chine real silk fabric is dropped in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 38 DEG C, incubation time 7 hours;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 25min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
embodiment 10:
Performance test:
Fabric Physical and mechanical performance: creasy recovery angle is pressed GB/T3819-1977 and measured, fracture strength is pressed GB/T39231997 and is measured.
Antibacterial fabric: adopt " shake flask test " test, antibiotic fabric and sample bacterium being added to fills in the flask of buffer solution, with oscillator below 25 degrees Celsius with after the velocity fluctuation 1h of 320-340r/min, calculate the viable count before and after vibration respectively, then be calculated as follows antibiotic rate: antibiotic rate=(A-B/A) * 100%
In formula: A is the viable count before vibration; B is the viable count after vibration.
Test strain: staphylococcus aureus.
Bacterial classification concentration: 6h nutrient solution dilutes 10,000 times.
Washability: with reference to home washings method, room temperature, carving board non-phosphide detergent, bath raio 1:30, washes by rubbing with the hands, each 3min of rinsing, as washing once, measures the clump count after washing several times and creasy recovery angle.
Blank group refers to untreated 02 crepe de Chine real silk fabric.
Initial testing result is as shown in table 1, and it is as shown in table 2 to wash 30 results, and it is as shown in table 3 to wash 60 results:
Table 1
Table 2
| Embodiment | Antibiotic rate (%) | Dry crease recovery angle（°） | Wet crease recovery angle（°） | Ultimate strength (N) | Gas permeability (/mm·s-1 ） |
| 2 | 98.2 | 319 | 218 | 427 | 90.0 |
| 3 | 99.1 | 309 | 229 | 433 | 86.2 |
| 4 | 99.2 | 321 | 223 | 429 | 88.9 |
| 5 | 98.5 | 315 | 220 | 435 | 90.2 |
Table 3
| Embodiment | Antibiotic rate (%) | Dry crease recovery angle（°） | Wet crease recovery angle（°） | Ultimate strength (N) | Gas permeability (/mm·s-1 ） |
| 2 | 98.1 | 318 | 217 | 426 | 89.9 |
| 3 | 99.0 | 307 | 227 | 432 | 86.1 |
| 4 | 98.9 | 319 | 221 | 428 | 88.8 |
| 5 | 98.2 | 313 | 219 | 433 | 90.1 |
As can be seen here, (the present inventor has also done other common bacterial strains a lot of to use other bacterial strain such as candida utili, all do not find there is significant modifying function to real silk), even be used alone Lactobacillus casei, Sphingol single-cell carries out microorganism modification silk fabric, do not possess good effect, visible Lactobacillus casei, serves synergy between Sphingol single-cell.
In addition, also can the effect of appreciable impact modification on the change of fermentation condition.
This illustrates, the selection of technology fungus kind of the present invention and zymotechnique are really established a capital and be and important.
The above; be only the present invention's preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (6)
1. the method for silk broadcloth microorganism modification:
(1) prepare composite bacteria agent capable: by Lactobacillus casei, Sphingol single-cell is mixed with composite bacteria agent capable;
(2) fermentation culture is configured: corn steep liquor, trehalose, shitosan, biotin, water, pH:7.0-7.5; Cool after sterilizing;
(3) ferment: silk fabric is dropped in fermentation culture, access bacteria fermentation;
(4) inactivation treatment: fabric is taken out in input 80 DEG C of water and keep 15min-30min, clear water rinsed clean;
(5) low temperature drying: 60 DEG C of low temperature dryings.
2. the method for silk broadcloth microorganism according to claim 1 modification, is characterized in that:
Step (1) described Lactobacillus casei, Sphingol single-cell mixes according to weight ratio 2-3:1-1.5.
3. the method for silk broadcloth microorganism according to claim 2 modification, is characterized in that:
Step (2) is: corn steep liquor 3-6g/L, trehalose 2-3g/L, shitosan 2-3g/L, biotin: 20ug/L, pH:7.0-7.5; 33 degree are cooled to after sterilizing.
4. the method for silk broadcloth microorganism according to claim 3 modification, is characterized in that:
Step (3) is: dropped into by silk fabric in fermentation culture, access bacteria fermentation, inoculum concentration 1%, fermentation temperature 37-39 DEG C, incubation time 5-15 hour.
5. the method for silk broadcloth microorganism according to claim 3 modification, is characterized in that:
Described silk fabric is 02 crepe de Chine real silk fabric.
6. the method for modifying described in claim 1-5 prepares modification silk fabrics.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560354A (en) * | 2004-02-20 | 2005-01-05 | 湖北省博物馆 | Microorganism reinforced method of non-strength silk |
| CN101492668A (en) * | 2009-03-10 | 2009-07-29 | 江苏明睿科技实业有限公司 | Process for producing biological modified carbonize stalk |
| CN101935952A (en) * | 2010-08-31 | 2011-01-05 | 天津工业大学 | Biological modification method of dacron fabrics |
| CN103031733A (en) * | 2012-12-03 | 2013-04-10 | 鑫缘茧丝绸集团股份有限公司 | Preparation method of anti-crease antimicrobial complex-function true silk |
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- 2015-09-17 CN CN201510593070.7A patent/CN105200799A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560354A (en) * | 2004-02-20 | 2005-01-05 | 湖北省博物馆 | Microorganism reinforced method of non-strength silk |
| CN101492668A (en) * | 2009-03-10 | 2009-07-29 | 江苏明睿科技实业有限公司 | Process for producing biological modified carbonize stalk |
| CN101935952A (en) * | 2010-08-31 | 2011-01-05 | 天津工业大学 | Biological modification method of dacron fabrics |
| CN103031733A (en) * | 2012-12-03 | 2013-04-10 | 鑫缘茧丝绸集团股份有限公司 | Preparation method of anti-crease antimicrobial complex-function true silk |
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