CN105193741B - A kind of preparation method of Yinqiao San solid decoction - Google Patents
A kind of preparation method of Yinqiao San solid decoction Download PDFInfo
- Publication number
- CN105193741B CN105193741B CN201510708399.3A CN201510708399A CN105193741B CN 105193741 B CN105193741 B CN 105193741B CN 201510708399 A CN201510708399 A CN 201510708399A CN 105193741 B CN105193741 B CN 105193741B
- Authority
- CN
- China
- Prior art keywords
- decoction
- medicinal material
- yinqiao san
- preparation
- yinqiao
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a kind of preparation method of Yinqiao San solid decoction, including：(1) by each medicinal material of preparation：Honeysuckle 30g, lophatherum gracile 12g, capsule of weeping forsythia 30g, schizonepeta 12g, Fermented Soybean 15g, great burdock achene 18g, licorice 15g, peppermint 18g, balloonflower root 18g and reed rhizome 18g；(2) each medicinal material is merged and is ground into coarse granule, diameter is not more than 1cm, is placed in boiling machine, adds water 90mL, and acc power of boiling medicine is 1200W, and pot first is preheated into 2min, is reheated after preheating and decocts 3min, obtains adsorbing the medicinal material coarse granule of decoction；After decoction is dried together with medicinal material, it is fitted into filter paper bag, it is outer directly to be packed with transparent plastic bag, produce Yinqiao San solid decoction.The advantage of the invention is that：The Yinqiao San solid decoction of the present invention has that decocting time is shorter, amount of water is less, and preparation method is simple, the advantages of carrying, be convenient to take.
Description
Technical field
Present invention relates particularly to a kind of preparation method of Yinqiao San solid decoction.
Background technology
Solid decoction is reformed on the basis of decoction with decoction made from powder agent, is a kind of easily and efficiently novel dosage forms, is to change
A kind of carrying, the solid system convenient to take that traditional decoction decoction method be combined with each other with modern mechanical automated production are entered
Agent, it not only has traditional decoction to absorb the characteristics of fast, drug effect is rapid, moreover it is possible to adds and subtracts flavour of a drug medicine with card according to the change of the state of an illness
Amount, can relatively comprehensive the characteristics of neatly taking into account different patients or different lesions stage；And carry, convenient to take, mechanization
Automatic production process can significantly decrease the time of boiling medicine, and improve the fried efficiency of medicine.
Yinqiao San is by honeysuckle, the capsule of weeping forsythia, licorice, great burdock achene, peppermint, schizonepeta, Fermented Soybean, lophatherum gracile, balloonflower root, reed rhizome
Ten taste medicines composition, wherein honeysuckle, the capsule of weeping forsythia are monarch drug in a prescription.Honeysuckle main component has chlorogenic acid compound, flavonoid
Thing and triterpene saponin componds and volatile oil etc.；The main component of the capsule of weeping forsythia is lignanoid and its glycosides compound, benzyl carbinol
Glycosides compound and pentacyclic triterpenoid and volatile oil etc..Chlorogenic acid is the principle active component of honeysuckle, have decompression,
Cholagogic, antibacterial, white effect is risen, while also there is oxidation resistant activity, be promising antioxidant.Forsythin is in the capsule of weeping forsythia
Important active material, and one of index composition of the capsule of weeping forsythia.Ministerial drug great burdock achene, peppermint etc. contain arctiin, orange in side
Skin glycosides, not only for active ingredient but also be detection index composition.Therefore the amount of these four materials in present invention measure Yinqiao San is simultaneously
As Con trolling index.
The content of the invention
The technical problem to be solved in the present invention, it is to provide a kind of preparation method of Yinqiao San solid decoction.
What the present invention was realized in：A kind of preparation method of Yinqiao San solid decoction, comprises the following steps：
(1) each medicinal material is prepared by formula as below：
It is honeysuckle 30g, lophatherum gracile 12g, capsule of weeping forsythia 30g, schizonepeta 12g, Fermented Soybean 15g, great burdock achene 18g, licorice 15g, thin
Lotus 18g, balloonflower root 18g and reed rhizome 18g；
(2) each medicinal material in (1) is merged and is ground into coarse granule, diameter is not more than 1cm, is placed in boiling machine, adds water
90mL, acc power of boiling medicine are 1200W, reheat decoction 3min after first preheating 2min, obtain adsorbing the medicinal material coarse granule of decoction；Will
After decoction is dried together with medicinal material, it is fitted into filter paper bag, it is outer directly to be packed with transparent plastic bag, produce Yinqiao San solid decoction.
Further, the method for pouring of the Yinqiao San solid decoction is as follows：
Brewed 2 times with boiling water 200mL, each brewing time is 5min.
The advantage of the invention is that：The Yinqiao San solid decoction of the present invention has that decocting time is shorter, amount of water is less, system
Make that method is simple, the advantages of carrying, be convenient to take.
Embodiment
A kind of preparation method of Yinqiao San solid decoction, comprises the following steps：
(1) each medicinal material is prepared by formula as below：
It is honeysuckle 30g, lophatherum gracile 12g, capsule of weeping forsythia 30g, schizonepeta 12g, Fermented Soybean 15g, great burdock achene 18g, licorice 15g, thin
Lotus 18g, balloonflower root 18g and reed rhizome 18g；
(2) each medicinal material in (1) is merged and is ground into coarse granule, diameter is not more than 1cm, is placed in boiling machine, adds water
90mL, acc power of boiling medicine are 1200W, reheat decoction 3min after first preheating 2min, obtain adsorbing the medicinal material coarse granule of decoction；Will
After decoction is dried together with medicinal material, it is fitted into filter paper bag, it is outer directly to be packed with transparent plastic bag, produce Yinqiao San solid decoction.
The method for pouring of the Yinqiao San solid decoction is as follows：
Brewed 2 times with boiling water 200mL, each brewing time is 5min.
1st, the preparation of Yinqiao San solid decoction test sample
Filtered after Yinqiao San solid decoction obtained above is brewed with boiling water 200mL, be settled to 250mL, be then evaporated,
Ultrasonic extraction 30min after addition methanol 25mL, precision measures 15mL filtrates after filtration, adds methanol constant volume to be shaken up, with note to 50mL
Emitter is produced by miillpore filter.
High performance liquid chromatography detection condition：
Chromatographic column：Ultimate XB-C18 (4.6mm × 250mm, 5 μm), Detection wavelength：279nm, column temperature are 30 DEG C, stream
Dynamic is mutually methanol：Phosphate aqueous solution, type of elution：Gradient elution, flow velocity 0.8mL/min, sample size are 10 μ L.
The gradient elution program of table 1
1.1 forsythins, chlorogenic acid, arctiin, the assay of aurantiamarin
1.1.1 the configuration of reference substance solution
Forsythin, chlorogenic acid, arctiin and aurantiamarin reference substance precision weigh in right amount, add methanol difference constant volume, system
Into reference substance solution, concentration is respectively：0.1428mg/mL, 0.1309mg/mL, 0.1536mg/mL and 0.1686mg/mL.
1.1.2 linear relationship is investigated
Precision is drawn in each reference substance solution 0.2mL, 0.6mL, 1.0mL, 1.5mL, 2.0mL, 3.0mL to 10mL measuring bottle
And add methanol constant volume.Draw 20 μ L injection high performance liquid chromatographs respectively again, record the integrating peak areas value of chromatogram measure,
And with reference substance sample introduction quality solubility (μ g/mL) for abscissa x, reference substance peak area is that ordinate y carries out linear regression, as a result
For：The regression equation of forsythin is y=6900.6x+4356.9, r=0.9994 (n=6), it was demonstrated that the sample size of forsythin exists
Relation is good in the μ g ranges of 1.3 μ g~45.4, and the regression equation of chlorogenic acid is y=82104x-54858, r=0.9998 (n=
6), it was demonstrated that the sample size of chlorogenic acid relation in the μ g ranges of 2.6 μ g~39.4 is good, and the regression equation of arctiin is y=
7214x-1532.3, r=0.9996 (n=6), it was demonstrated that the sample size of arctiin relation in the μ g ranges of 3.2 μ g~44.0 is good
Good, the regression equation of aurantiamarin is y=7411.5x-2013.5, r=0.9998 (n=6), it was demonstrated that the sample size of aurantiamarin exists
Relation is good in the μ g ranges of 3.4 μ g~51.0.
The linear relationship of the forsythin of table 2
The linear relationship of the chlorogenic acid of table 3
The linear relationship of the arctiin of table 4
The linear relationship of the aurantiamarin of table 5
1.1.3 Precision Experiment
Take Yinqiao San solid decoction need testing solution continuous sample introduction 5 times, and calculate forsythin, chlorogenic acid, arctiin, orange
Skin glycosides peak area RSD%, as a result it is respectively：0.29%th, 0.97%, 0.64% and 1.02%, show that instrument precision is good.
The Precision Experiment result of table 6
1.1.4 repeated experiment
5 parts of sample introductions of Yinqiao San solid decoction test sample are prepared, and calculate forsythin, chlorogenic acid, arctiin and aurantiamarin
Peak area RSD%, as a result it is respectively：1.04%th, 0.33%, 0.84% and 1.17%, show that method repeatability is good.
The repeated experiment result of table 7
1.1.5 stability test
Take Yinqiao San solid decoction need testing solution respectively in 0h, 2h, 4h, 6h, 8h, 12h, 24h sample introduction once, and
The RSD of chlorogenic acid, forsythin, arctiin and aurantiamarin peak area is calculated, as a result respectively 0.82%, 0.59%, 0.34%
With 0.54%, show：Sample is stable in 24h.
The stability experimental result of table 8
1.1.6 recovery test
6 parts of the Yinqiao San solid decoction with a collection of known content is taken, precision adds reference substance in right amount and grasped by " 1.1.3 "
Make, determine content.As a result the average recovery rate of forsythin, chlorogenic acid, arctiin and aurantiamarin be respectively 97.50%,
99.44%th, 100.67% and 100.56%, RSD be respectively 4.29%, 1.37%, 2.71% and 2.50%.
The forsythin determination of recovery rates result of table 9
The chlorogenic acid determination of recovery rates result of table 10
The arctiin determination of recovery rates result of table 11
The aurantiamarin determination of recovery rates result of table 12
1.2 influence the investigation of Yinqiao San solid decoction preparation factor
Because Yinqiao San includes volatile oil, original side's decoction made from powder decocting time is also shorter, and also needs boiling water to brew when taking.Therefore set
Decocting time is 3min, because acc power of boiling medicine is fixed as 1200W, therefore probes into amount of water herein to each main component and yield of extract
Influence.Remaining decoction drying time speed comprehensive descision preferred plan after decocting is considered simultaneously, the results are shown in Table 2-13.
1.2.1 yield of extract calculates
Yinqiao San solid decoction is brewed with 90 DEG C of running water 200mL, constant volume measures filtrate 20mL in 250mL after filtering
It is placed in having dried water bath method in the evaporating dish of constant temperature, is positioned over after being evaporated in 105 DEG C of vacuum drying chambers and dries 3h, after taking-up
It is put into 30min in drier, and precise weighing.
(for m to measure medicinal extract weight, M is pharmaceutical decocting piece gross weight)
The Yinqiao San solid decoction amount of water investigation table of table 13
As shown in Table 13 it is fixed decoct power and decocting time under conditions of, because amount of water only differs 5mL, to it is each into
Point content and soft extracts yield influence little, but consider to decoct overdrying and whether have decoction and do on the bottom of a pan and the length of drying time
It is short, show that optimal decoction scheme is：Decoct 3 minutes, decoct temperature (power 1200W), amount of water 90mL.
The confirmatory experiment of 1.3 optimum conditions
Pharmaceutical decocting piece is weighed by recipe quantity and is ground into the thick end of 1cm sizes, and Yinqiao San is prepared under optimum process condition
Solid decoction, repeat to prepare 3 times, determine, average according to the method described above, the results are shown in Table 14.
The optimised process the result of table 14
1.4 molding research
Yinqiao San solid decoction is prepared according to preferable the best conditions of preparation pr ocess, is put into filter paper bag, and packed.
1.4.1 orthogonal experiment optimization solid decoction brews technique
Because solid decoction is the pharmaceutical decocting piece through decocting and being dried together with concoction, mixing in water for oral taking need to be used, therefore to solid
The method for pouring of decoction does simple optimizing, as solid decoction medication guide.It is 2 times according to the decoction number of traditional decoction, Gu
Fixed solid decoction brews number twice, (the different boiling points for causing water of different regions height above sea level to be considered for 90 DEG C using water
It is different), investigated by factor of probing into of amount of water, the results are shown in Table 15.
The solid decoction of table 15 brews process results
As shown in Table 15, amount of water when brewing has influenceed on brewing effect, and it is 200mL most preferably to brew amount of water.Most
The good scheme that brews is：90 DEG C of water temperature, 200mL are brewed 2 times.
1.4.2 the confirmatory experiment of optimum condition
Pharmaceutical decocting piece is weighed by recipe quantity and is ground into the thick end of 1cm sizes, and Yinqiao San is prepared under optimum process condition
Solid decoction, and brewed under optimal brewing conditions, repeat experiment 3 times and be measured, average, as a result see
Table 16.
The optimised process the result of table 16
2. Yinqiao San and its solid decoction yield of extract compare
2.1 assay method
According to《Pharmacopoeia of People's Republic of China》Version water-soluble extractives assay method in 2010：Drawn with pipette is accurate
Yinqiao San decocts gained decoction 10mL, is placed in the evaporating dish for drying constant temperature, is put into after water bath method 105 in vacuum drying chamber
DEG C 3h is dried under reduced pressure, is put into drier precise weighing after 0.5h.Calculate total medicinal extract weight and yield of extract.
2.2 leaching rate computational methods
By the Yinqiao San solid decoction of the present invention from adding 200mL water to brew respectively the 1st, 2,3,5,10,20,30,
20mL is accurately drawn during 60min time intervals, is centrifuged after being supplemented every time with water 20mL, absorption 10mL supernatant liquors, which are placed in, have been dried
In the evaporating dish of constant temperature, water bath method is dried under reduced pressure 3h after 105 DEG C in vacuum drying chamber, is weighed after being placed in drier 0.5h.
M is to measure solid weight g (m values first
Secondary is m1, and second is m1+m2, by that analogy), mLAlwaysIt is the total amount (mL) of volume, mLInspectionIt is the liquid measure to be checked being measured
(mL), W is example weight (g), m(1~8)Thing weight (g) is always measured for m.
2.3 sample preparation
2.3.1 Yinqiao San decoction made from powder sample preparation
Pharmaceutical decocting piece is weighed by recipe quantity and is ground into the thick end of 1cm sizes, is placed in boiling machine and is decocted 2 times (for the first time
Add water 300mL, decoct 3mim, obtain decocting liquid about 200mL, the 2nd time plus water 300mL, obtain decocting liquid about 200mL), merge decocting liquid twice,
400mL is settled to after filtering.20mL is taken after water bath method, to be put into infrared drying oven 105 DEG C in the evaporating dish for drying constant temperature
0.5h in drier, precise weighing are put after drying 3h.And 3 parts are prepared by the above process.
2.3.2 Yinqiao San brews scattered preparation
Pharmaceutical decocting piece is weighed by recipe quantity and is ground into the thick end of 1cm sizes, after brewing 2 times with 90 DEG C of running water of 200mL
Filtering, is settled to 400mL.Take 20mL in the evaporating dish for drying constant temperature after water bath method, be put into infrared drying oven 105 DEG C it is dry
0.5h in drier, precise weighing are put after dry 3h.3 parts are prepared by the above process.
2.3.3 prepared by Yinqiao San solid decoction
Pharmaceutical decocting piece is weighed by prescription and is ground into the thick end of 1cm sizes, in boiling machine, is added water 90mL, is decocted 3 points
Clock, filtered after brewing 2 times with 90 DEG C of running water of 200mL after drying 12 minutes, be settled to 400mL.20mL is taken in dry constant temperature
In evaporating dish after water bath method, it is put into infrared drying oven and 0.5h in drier is put after 105 DEG C of dry 3h, precise weighing, prepares 3
Part.
2.3.4 measurement result
The yield of extract measurement result of the Yinqiao San of table 17 and its solid decoction
The leaching rate measurement result of the Yinqiao San solid decoction of table 18
2.3.5 statistical disposition result
T inspections are carried out using SPSS10.0 statistical softwares to the yield of extract of Yinqiao San and its solid decoction and (take conspicuousness α
=0.05) it is, as a result as follows：
Homogeneity test of variance F=2.1683<F0.5/2 (2,2) is P>0.05, then variance is neat
Average t two-tailed tests t0.1/2.2<T=2.5348<T0.2/2.2 is P>0.05 is not statistically significant, it is believed that
The Yinqiao San solid decoction of the present invention does not have difference with the yield of extract of Yinqiao San original side decoction made from powder, and medicinal extract is the total of clinical practice
Material base, the basic difference of the two can be reflected.
In summary, influenceing the factor of Yinqiao San solid decoction preparation technology mainly has decoction temperature, amount of water, because used in
Boiling machine is constant power, therefore temperature factor is not discussed, is by the optimum process condition that goes out of experiment：Amount of water 90mL, decoct
Time 3min, technique is most preferably brewed to add boiling water (water for 90 DEG C herein) 250mL to brew 2 times.
Test result indicates that the preparation technology of Yinqiao San solid decoction and brewing process stabilizing, convenient, make on this condition
There was no significant difference with Yinqiao San for standby Yinqiao San solid decoction yield of extract.
The Yinqiao San solid decoction of the present invention has that decocting time is shorter, amount of water is less, preparation method is simple, carry,
The advantages of convenient to take, the present invention in traditional decoction with being improved in decoction made from powder agent preparation method, the system of Yinqiao San solid decoction
Preparation Method is identical with traditional decoction, and simply dosage form and decocting condition difference, are solid decoction and original side's slice decoction
Chemical composition contrast laid the foundation with study on the efficiency.
Claims (2)
- A kind of 1. preparation method of Yinqiao San solid decoction, it is characterised in that：Comprise the following steps：(1) each medicinal material is prepared by formula as below：Honeysuckle 30g, lophatherum gracile 12g, capsule of weeping forsythia 30g, schizonepeta 12g, Fermented Soybean 15g, great burdock achene 18g, licorice 15g, peppermint 18g, balloonflower root 18g and reed rhizome 18g；(2) each medicinal material in (1) is merged and is ground into coarse granule, diameter is not more than 1cm, is placed in boiling machine, adds water 90mL, decocts Medicine acc power is 1200W, reheats decoction 3min after first preheating 2min, obtains adsorbing the medicinal material coarse granule of decoction；By decoction with After medicinal material is dried together, it is fitted into filter paper bag, it is outer directly to be packed with transparent plastic bag, produce Yinqiao San solid decoction.
- A kind of 2. preparation method of Yinqiao San solid decoction as claimed in claim 1, it is characterised in that：The Yinqiao San solid The method for pouring of decoction is as follows：Brewed 2 times with boiling water 200mL, each brewing time is 5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510708399.3A CN105193741B (en) | 2015-10-27 | 2015-10-27 | A kind of preparation method of Yinqiao San solid decoction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510708399.3A CN105193741B (en) | 2015-10-27 | 2015-10-27 | A kind of preparation method of Yinqiao San solid decoction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105193741A CN105193741A (en) | 2015-12-30 |
| CN105193741B true CN105193741B (en) | 2018-02-16 |
Family
ID=54941940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510708399.3A Expired - Fee Related CN105193741B (en) | 2015-10-27 | 2015-10-27 | A kind of preparation method of Yinqiao San solid decoction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105193741B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108607046A (en) * | 2016-12-13 | 2018-10-02 | 河南后羿实业集团有限公司 | A kind of animal compound Lonicera and Forsythia oral solution and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150900A (en) * | 1995-11-22 | 1997-06-04 | 张维安 | Production technology of traditional Chinese medicine solid decoction |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5926258B2 (en) * | 1982-09-29 | 1984-06-26 | 欣一 林 | Method for producing medicinal syrup |
-
2015
- 2015-10-27 CN CN201510708399.3A patent/CN105193741B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150900A (en) * | 1995-11-22 | 1997-06-04 | 张维安 | Production technology of traditional Chinese medicine solid decoction |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105193741A (en) | 2015-12-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107149623B (en) | Content determination method of traditional Chinese medicine composition | |
| CN107153098B (en) | Method for measuring fingerprint spectrum of traditional Chinese medicine composition | |
| CN101850070B (en) | Detection method for Chinese medicament Tangcao tablets | |
| CN101732607B (en) | Method for detecting quality of huaqi Chinese medicinal preparation | |
| CN106226440B (en) | The method of quality control of Shengma Gegen Tang composition | |
| CN106822203B (en) | Radix angelicae pubescentis granules and preparation method and quality control method thereof | |
| CN109085256A (en) | HPLC method that is a kind of while detecting 11 kinds of ingredients in Radix Rehmanniae rheum officinale | |
| CN110118846A (en) | The method for building up of Taohe Chengqi decoction matter basis | |
| CN107589191A (en) | Golden shellfish oral liquid HPLC finger-prints are established and detection method | |
| CN108459128B (en) | Quality control method of angelica sinensis Sini decoction composition | |
| CN103197026A (en) | Quality control method of granules capable of strengthening and consolidating body resistance | |
| CN113281439B (en) | Quality control detection method of Shenbao tablets | |
| CN103115984A (en) | Quality control method of medicament for treating leukopenia | |
| CN112666277B (en) | HPLC (high Performance liquid chromatography) characteristic spectrum construction and detection method for rhizoma cyperi medicinal materials, decoction pieces, standard decoction and formula granules | |
| CN105193741B (en) | A kind of preparation method of Yinqiao San solid decoction | |
| CN104013673B (en) | A kind of Salvia root P.E and preparation method thereof | |
| CN113759035A (en) | Method for constructing fingerprint of Xiaochengqi decoction | |
| CN103575821A (en) | Detection method of 14 chemical components in Tangminling preparation | |
| CN103230453A (en) | Yinchengao Decoction formula particles, and preparation method and detection method thereof | |
| CN103604898B (en) | The benefit heart relaxes the fingerprint atlas detection method of preparation | |
| CN101028474B (en) | Method for inspecting the quality of Chinese preparation with Yang-and kidney tonifying functions | |
| CN106706766A (en) | Method for detecting newly increased detection components in traditional Chinese medicinal Tang Herb for treating AIDS | |
| CN105067725B (en) | It is a kind of at the same determine shenmei stomach nourishing granule in the root of herbaceous peony, radix glycyrrhizae and the red sage root content method | |
| CN104914186B (en) | The detection method of three kinds of compositions such as hard bar Leontopodium leontopodioides (Willd.) Beauv. or its processed product Content of Chlorogenic Acid | |
| CN108982731B (en) | Method for constructing HPLC characteristic spectrum of sarcandra glabra buccal tablet and HPLC characteristic spectrum thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180216 Termination date: 20211027 |