CN105193520A - Method for fixing eyeball of living mouse - Google Patents
Method for fixing eyeball of living mouse Download PDFInfo
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- CN105193520A CN105193520A CN201510638142.5A CN201510638142A CN105193520A CN 105193520 A CN105193520 A CN 105193520A CN 201510638142 A CN201510638142 A CN 201510638142A CN 105193520 A CN105193520 A CN 105193520A
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- mouse
- eyeball
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- snare
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Abstract
A method for fixing the eyeball of a living mouse comprises the steps that 1, the mouse is anesthetized with 4.3% chloral hydrate based on the rate of 0.1ml/10g, and it can be observed that all muscles of the mouse relax, breathing is steady, and corneal reflex is blunt five minutes after anesthetization; 2, the limbs of the mouse are fixed to a surface plate with clips, and the area around the eyelid of the mouse is scrubbed with iodophor; 3, under an electron microscope, a handle of a mouse eyeball snare is held with the index finger and thumb of the left hand, the annular structure of the mouse eyeball snare is pressed down from top to bottom right facing the upper surface of the eyelid of the mouse, the eyeball of the mouse is trapped and fixed by the annular structure after the eyelid of the mouse is pushed away by the annular structure, the eyeball of the mouse protrudes upwards out of the plane of the annular structure at the moment, and the annular structure is pressed downwards continuously till veins on the sclera of the eyeball of the mouse are exposed clearly.
Description
Technical field
The present invention relates to a kind of method of fixing intravital mouse eyeball.
Background technology
For various reasons, need in the enterprising line operate of intravital mouse eyeball and process, as measured mice intraocular pressure, to the eyeball of mouse surface observation of each organ-tissue structure, measurement and experiment, comprising and sclera, sclera upper surface vein etc. on eyeball of mouse are carried out, measuring, test or other disposal follow-up.Because eyeball of mouse is less, and eyeball meeting 360 degree of rotations, so in the unfixed situation of eyeball, cannot subsequent operation accurately be carried out.
Summary of the invention
The present invention aims to provide a kind of fixing intravital mouse eyeball method.
The instrument that first place of matchmakers uses, namely a kind of eyeball of mouse snare described in Chinese patent ZL201520730313.2, comprise the vertical shape handle of stainless steel and be connected to the circulus of handle tip, described circulus diameter is the several specification of 2mm, 2.5mm, 3mm, 3.5mm, 4mm, 4.5mm; Described handle and circulus are stainless steel.The material diameter that described circulus uses is 250 μm, and the material diameter that handle uses is for being 2mm, the long 60mm of handle.
Be below used fixing intravital mouse eyeball method:
A method for fixing eyeball of mouse, is characterized in that following steps:
1,4.3% chloral hydrate presses 0.1ml/10g anesthetized mice, observes mouse systemic of flaccid muscles after 5 minutes, and breathe steadily, corneal reflex is blunt.
2, mice extremity clip is fixed on surface plate, povidone iodine wiping mice surrounding eyelids.
3, under ultramicroscope, left index finger and thumb pinch the handle of eyeball of mouse snare, the circulus of eyeball of mouse snare is just pressed mice eyelid upper surface from the top down, snare after circulus squeezes mice eyelid also fixes eyeball of mouse, now eyeball of mouse is protruding upward in circulus plane, continue circulus to press downwards, until can on clear exposure eyeball of mouse sclera vein till.
So far, intravital mouse eyeball is fixed complete.
Further, described step 1 adopts gas or takes drug anesthesia
Further, described step 1 is omitted.
Further, between step 2 and 3, inserting step 2.5: be suspended from above intravital mouse eyeball with slide gauge, sight line is perpendicular to slide gauge, measure the diameter intending fixing eyeball of mouse, then select diameter slightly larger than or equal the eyeball of mouse snare of eyeball of mouse diameter specifications.
The eyeball of mouse snare of this kind of shape, its handle add circulus other industry and technical field very common.Circulus is used for medical model and makes field, be specially fixing intravital mouse eyeball, never someone did.Due to eyeball of mouse diameter very little (2-4mm), and intravital mouse eyeball is surrounded by upper palpebra inferior.Common thinking intravital mouse eyelid pincet or Small clamp is clamped to separate up and down afterwards, then fixed by eyeball of mouse.And mice eyelid thickness is very thin and area is very little (2-4 square millimeter), therefore clamp the operation of intravital mouse eyelid very not easily.Therefore the technical barrier that intravital mouse eyeball is this field is always fixed.The present invention creatively opens the new approaches addressed this problem, by the circulus of eyeball of mouse snare directly by being pressed in intravital mouse eyelid upper surface, now circulus first contacts mice eyelid, because mice eyelid thickness is very thin and area is little (2-4 square millimeter), therefore intravital mouse eyelid cannot bear the effect of slightly larger external force.After the ring-type downforce of intravital mouse eyelid by circulus applying, namely the eyelid of intravital mouse scatters to surrounding and exposes eyeball.Now the circulus of eyeball of mouse snare direct snare of can taking advantage of a situation lives eyeball of mouse, when circulus continues to press, eyeball of mouse is protruding upward in circulus plane, after circulus presses downwards by continuation, mice eyelid completely and eyeball of mouse separate, and circulus has fixed the eyeball of intravital mouse.Now on eyeball of mouse, the vein of sclera can clearly expose, and facilitates later observation and disposal.
Circulus directly by being pressed in mice eyelid upper surface, is squeezed eyelid by employing, takes advantage of a situation and directly fixes the method for intravital mouse eyeball.Before the method, never someone used, and had outstanding substantive distinguishing features.
The creationary circulus that other field is conventional of the method is used in fixing intravital mouse eyeball field.The method is with low cost, simple to operate, success rate 100%.The complex process of eyeball of mouse operational approach and the extremely low shortcoming of success rate is fixed with clip before overcoming.Achieve good technique effect.There is significant progress.
And the technique effect that the method obtains also is that industry technical staff is not easy to expect in advance, has unforeseeable technique effect.The first separately eyelid that general those skilled in the art process that this technical problem always expects, then fix the thinking of eyeball.The invention provides the technical scheme that a kind of technical conceive is different, by creatively using this instrument of circulus in other technologies field, the eyelid of Continued depression intravital mouse, the process being separated and fixing eyeball of mice eyelid and eyeball is accomplished without any letup, and be separated eyelid, the process of fixing eyeball can not produce injury to intravital mouse.Its technique effect can exceed the level of prior art.Therefore the present invention is concerning creative prior art.
Detailed description of the invention
A method for fixing intravital mouse eyeball, is characterized in that following steps:
1,4.3% chloral hydrate presses 0.1ml/10g anesthetized mice, observes mouse systemic of flaccid muscles after 5 minutes, and breathe steadily, corneal reflex is blunt.
2, mice extremity clip is fixed on surface plate, povidone iodine wiping mice surrounding eyelids.
Fixing eyeball of mouse diameter is intended in A, range estimation, is then adjusted to by the diameter of described circulus more bigger or consistent than eyeball of mouse diameter with tweezers or needle-nose pliers.
3, under ultramicroscope, left index finger and thumb pinch the hands handle of eyeball of mouse snare, the circulus of eyeball of mouse snare is just pressed mice eyelid upper surface from the top down, by circulus directly by when being pressed in mice eyelid upper surface, circulus first contacts mice eyelid, because mice eyelid is very thin and area is little (2-4 square millimeter), therefore circulus can squeeze mice eyelid and directly snare lives eyeball of mouse, snare after fixing eyeball of mouse, now eyeball of mouse is protruding upward in circulus plane, circulus is continued to press downwards, mice eyelid completely and eyeball of mouse separate, until can on clear exposure eyeball of mouse sclera vein till.
So far, eyeball of mouse is fixed complete.
Claims (4)
1. a method for fixing intravital mouse eyeball, is characterized in that comprising following steps:
1) 4.3% chloral hydrate presses 0.1ml/10g anesthetized mice, observes mouse systemic of flaccid muscles after anaesthetizing latter 5 minutes, and breathe steadily, corneal reflex is blunt;
2) mice extremity clip is fixed on surface plate, povidone iodine wiping mice surrounding eyelids;
3) under ultramicroscope, left index finger and thumb pinch the handle of eyeball of mouse snare, the circulus of eyeball of mouse snare is just pressed mice eyelid upper surface from the top down, snare after circulus squeezes mice eyelid also fixes eyeball of mouse, now eyeball of mouse is protruding upward in circulus plane, continue circulus to press downwards, until can on clear exposure eyeball of mouse sclera vein till;
So far, eyeball of mouse is fixed complete.
2. the method for a kind of fixing intravital mouse eyeball as claimed in claim 1, is characterized in that described step 1 adopts gas or takes drug anesthesia.
3. the method for a kind of fixing intravital mouse eyeball as claimed in claim 1, is characterized in that described step 1 is omitted.
4. the method for a kind of fixing intravital mouse eyeball as claimed in claim 1, it is characterized in that between step 2 and 3, inserting step 2.5: be suspended from above intravital mouse eyeball with slide gauge, sight line is perpendicular to slide gauge, measure the diameter intending fixing eyeball of mouse, then select diameter slightly larger than or equal the eyeball of mouse snare of eyeball of mouse diameter specifications.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510638142.5A CN105193520A (en) | 2015-09-30 | 2015-09-30 | Method for fixing eyeball of living mouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510638142.5A CN105193520A (en) | 2015-09-30 | 2015-09-30 | Method for fixing eyeball of living mouse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105193520A true CN105193520A (en) | 2015-12-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510638142.5A Pending CN105193520A (en) | 2015-09-30 | 2015-09-30 | Method for fixing eyeball of living mouse |
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| CN (1) | CN105193520A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107802372A (en) * | 2017-10-19 | 2018-03-16 | 中国科学院深圳先进技术研究院 | A kind of animal eyeball fixing device for photoacoustic imaging system |
| CN111110441A (en) * | 2020-01-08 | 2020-05-08 | 孙河 | Experimental rat sclera intravenous injection device and method |
-
2015
- 2015-09-30 CN CN201510638142.5A patent/CN105193520A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107802372A (en) * | 2017-10-19 | 2018-03-16 | 中国科学院深圳先进技术研究院 | A kind of animal eyeball fixing device for photoacoustic imaging system |
| CN111110441A (en) * | 2020-01-08 | 2020-05-08 | 孙河 | Experimental rat sclera intravenous injection device and method |
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Application publication date: 20151230 |