CN105181663B - Portable biochemical rapid detection system based on Quantum Dot Labeling - Google Patents

Abstract

The invention discloses a portable biochemical rapid detection system based on quantum dot fluorescence label. Including fluorescence detection module, optical fiber control module, automatic sampling module, rotary detection platform module, motor control module and host computer module, fluorescence detection module, optical fiber control module, automatic sampling module, rotary detection platform module and motor control module composition detection As the hardware part of the whole detection system, the motor control module is connected with the optical fiber control module, the automatic sampling module and the rotary detection platform module, the fluorescence detection module is connected with the fiber optic control module, and the host computer module is connected with the fluorescence detection module and the motor respectively. Control module connection. The invention realizes automation and high throughput in the process of quantitatively detecting biochemical samples by the fluorescent labeling method, has high measurement precision, fast detection speed, requires few samples, and is small in size and easy to carry.

Description

Portable biochemical rapid detection system based on quantum dot fluorescent labeling

technical field

The invention relates to the field of biochemical sample detection instruments, in particular to a portable biochemical rapid detection system based on quantum dot fluorescent labeling, which combines the quantum dot fluorescent label analysis method with mechanical and electronic control to form an automatic detection device system.

Background technique

Fluorescence is a phenomenon of photoluminescence. When a light source shines on certain substances, the so-called fluorescein, it emits excited light of different wavelengths; when the light source is removed, the excited light emitted by fluorescein also disappears. Using fluorescein to mark the analyte by certain means, and then based on the linear relationship between the fluorescence intensity emitted by fluorescein and the concentration of the analyte, is the detection principle of the so-called fluorescence spectroscopic analysis.

Compared with other methods, fluorescence spectroscopy has the advantages of high sensitivity, good specificity, and rapid detection, but the nature of fluorescein itself will have a relatively large impact on the detection results. The fluorescence lifetime is short or the wavelength is similar to the background light. have a negative impact on the final test results.

As an emerging fluorescent labeling material, quantum dots have many advantages over traditional materials. For example, it has a wide excitation spectrum and a narrow emission spectrum, so a variety of quantum dots can be simultaneously excited with the same excitation light source, each emitting fluorescence of different wavelengths, and then detected at the same time. Quantum dots also have a relatively large Stokes shift, which can obviously avoid the spectral peak overlap between the excitation light and the emission light. In addition, quantum dots have a relatively long fluorescence lifetime, and have relatively good biocompatibility.

For example, in the rapid detection of multiple food-borne pathogens, quantum dots are used as markers, and the method of fluorescent immunoassay has achieved relatively good laboratory results. For example, Yang and Li (2006) used two kinds of quantum dots with emission wavelengths of 525nm and 705nm to respectively label E. coli and Salmonella antibodies for detection. Wang et al. (2011) used three kinds of quantum dots with emission wavelengths of 530nm, 580nm, and 620nm As a fluorescent marker, it can detect Salmonella typhimurium, Escherichia coli 0157:H7 and Listeria monocytogenes at the same time, and has a relatively good detection effect.

Contents of the invention

The purpose of the present invention is to address the deficiencies of the prior art, to provide a method of using quantum dots as markers, based on a fluorescence analysis method, combined with mechatronic and computer software control technology, to realize automatic processing of one or more biochemical mixed samples. Device system for rapid detection.

In order to achieve the above object, the technical solution adopted in the present invention mainly includes:

The invention includes a fluorescence detection module, an optical fiber control module, an automatic sampling module, a rotary detection platform module, a motor control module and a host computer module, a fluorescence detection module, an optical fiber control module, an automatic sampling module, a rotary detection platform module and a motor control module The detection platform device is composed, the motor control module is connected with the optical fiber control module, the automatic sample injection module and the rotation detection platform module, the fluorescence detection module is connected with the optical fiber control module, the host computer module is connected with the fluorescence detection module and the motor control module respectively, and the upper position The machine module adopts a computer.

The rotary detection platform module includes a disposable syringe fixing plate, a cylindrical connector, a disposable test tube fixing plate, a positioning pin of the rotary testing platform, a second photoelectric switch, a second photoelectric switch fixing seat, a rotary driving block, a thrust ball bearing, The thrust ball bearing steel ball cage fixed ring and the driving motor of the rotating detection table; The square rotating drive block is coaxially fixedly connected, and the rotating drive block is set in the square hole in the center of the bottom surface of the disposable test tube fixed plate. There is a thrust ball bearing connected between the disposable test tube fixed plate and the top surface of the test table base The outer circumference is fixed by the radial limit of the thrust ball bearing steel ball cage fixing ring; the top of the disposable test tube fixing plate is vertically equipped with multiple disposable test tubes at intervals along the circumference, and the center of the top of the disposable test tube fixing plate passes through the cylindrical connector It is coaxially and fixedly connected with the disposable syringe fixing plate, and the disposable syringe corresponding to each disposable test tube is vertically arranged on the disposable syringe fixing plate at intervals along the circumference, and the needle of the disposable syringe is inserted downward into the disposable test tube; The second photoelectric switch is located on one side of the disposable test tube fixed plate, the second photoelectric switch is fixed on the base of the detection table through the second photoelectric switch fixing seat, and the positioning pin of the rotating detection table is fixed and installed on the bottom of the disposable test tube fixed plate along the radial direction The peripheral surface of the rotating detection table coordinates with the second photoelectric switch, and the optical fiber control module and the automatic sampling module are installed on the detection table base on the side of the disposable test tube fixed disk.

The automatic sampling module includes a linear guide rail, a lifting slider, a disposable syringe compression push plate, a lifting slider positioning trigger plate, a third photoelectric switch, a third photoelectric switch fixing plate, a fourth photoelectric switch, a fourth photoelectric switch The fixed plate, the driving motor of the automatic sampling module and the screw rod; the driving motor of the automatic sampling module is fixedly installed on the bottom surface of the detection table base, and the output shaft of the automatic sampling module driving motor passes through the through hole of the detection table base upwards and is the same as the screw rod. The shaft is fixedly connected, the side of the screw rod is vertically and parallelly installed with a linear guide rail, the screw rod is covered with a lifting slider embedded in the linear guide rail to move up and down, and the side of the lifting slider is fixedly connected with a lifting slider positioning trigger plate and a disposable syringe compression push plate; the third photoelectric switch and the fourth photoelectric switch are fixedly installed on the frame of the detection table through the third photoelectric switch fixing plate and the fourth photoelectric switch fixing plate respectively, and are located on the same side of the lifting slider positioning trigger plate, and the lifting slider The positioning trigger plate cooperates with the third photoelectric switch and the fourth photoelectric switch; the top of the disposable syringe compression push plate is provided with an extension rod facing the rotary detection platform module, and the station directly below the extension rod is the station where the disposable syringe is located. Press the disposable syringe.

The optical fiber control module includes a first photoelectric switch, a first photoelectric switch fixing seat, an optical fiber detection head mounting seat, an optical fiber, a positioning bolt of the optical fiber detection head mounting seat, and an optical fiber detection head drive motor, and the optical fiber detection head drive motor is fixedly installed in the detection On the bottom surface of the table base, the output shaft of the optical fiber detection head drive motor passes through the through hole of the detection table base and is coaxially fixedly connected with the optical fiber detection head mounting seat. The lower part of the optical fiber detection head mounting seat is horizontally installed with the positioning bolts of the optical fiber detection head mounting seat parallel to the optical fiber. The first photoelectric switch is installed on the side of the optical fiber detection head mounting seat through the first photoelectric switch fixing seat. The first photoelectric switch cooperates.

When the positioning bolt of the optical fiber detection head mounting seat is driven by the drive motor of the optical fiber detection head to rotate to the detection port where the first photoelectric switch is located, the optical fiber is aligned with the disposable test tube on the rotating detection platform module.

The fluorescence detection module includes a fluorescence excitation light source and a fluorescence detector, and the fluorescence excitation light source and the fluorescence detector are connected to the optical fiber of the optical fiber control module.

The optical fiber of the optical fiber control module is a Y-shaped optical fiber, and the bifurcated ends of the optical fiber are respectively connected to the fluorescence excitation light source and the fluorescence detector, and one end of the assembly is aligned with the bottom of the disposable test tube containing the liquid to be tested on the rotating detection platform module.

The motor control module includes a motor controller and three motor drivers, the motor controller is connected to the three motor drivers, and the three motor drivers are respectively connected to three of the optical fiber control module, the automatic sampling module and the rotation detection platform module of the present invention. connected to the motor.

The motor controller outputs PWM pulses to the motor driver to control the motion state of the motor.

As the main software component of the entire detection system, the upper computer module communicates with the detection device through USB or UART to control the progress of the entire detection process, and receives the spectral data obtained from the detection, and then analyzes and processes it, and finally obtains Get the test result. On the one hand, the upper computer module controls the motor control module and then controls the three motors in the automatic sampling module, optical fiber control module and rotation detection platform module. On the other hand, control the action of the fluorescence detection module, including collecting spectral signals, and then receive the obtained spectral data and perform filtering, peak-finding and other analysis and processing to obtain the real-time display of the detection results and generate the output of the detection results.

The beneficial effects that the present invention has are:

1. The present invention realizes the automation of the detection process of the quantum dot fluorescence labeling method. The operation is simple and convenient, which reduces the possible errors and cross-infection caused by human operation.

2. The syringes and test tubes used in the present invention are all disposable, which avoids the problem of easy residues in biochemical detection because it is difficult to clean thoroughly, and improves the accuracy of detection.

3. The rotary detection platform module of the present invention can realize the common detection of multiple samples, which improves the detection efficiency.

4. The present invention uses quantum dots with a wide excitation spectrum and a narrow emission spectrum as fluorescent analysis markers, so that only one light source can be used to excite multiple quantum dots, which simplifies the optical structure of the system; on the other hand, because quantum The narrow emission spectrum of the point, the present invention can also detect the mixed sample, which broadens the scope of application of the present invention.

5. The present invention has simple structure, small volume and good portability.

6. The quantum dot fluorescent labeling method adopted in the present invention is relatively simple, and the detection time is relatively short and the accuracy is high.

Description of drawings

Fig. 1 is a summary frame structure diagram of the present invention.

Fig. 2 is a schematic diagram of the mechanical structure module of the present invention.

Fig. 3 is a structural diagram of the rotation detection platform module of the present invention.

Fig. 4 is a structural diagram of the automatic sampling module of the present invention.

Fig. 5 is a structural diagram of an optical fiber control module of the present invention.

Fig. 6 is a structural diagram of the fluorescence detection module of the present invention.

Fig. 7 is a schematic diagram of the structural connection of the motor control module of the present invention.

Fig. 8 is a detection flow chart of the present invention.

In the figure: detection table base 1, first photoelectric switch fixing seat 2, first photoelectric switch 3, optical fiber detection head mounting seat 4, optical fiber 5, disposable test tube fixing plate 6, cylindrical connector 7, disposable syringe fixing plate 8 , linear guide rail 9, lifting slider 10, disposable syringe compression push plate 11, lifting slider positioning trigger plate 12, third photoelectric switch 13, third photoelectric switch fixing block 14, fourth photoelectric switch fixing block 15, fourth Photoelectric switch 16, disposable syringe 17, disposable test tube 18, positioning pin 19 of the rotary detection table, second photoelectric switch 20, second photoelectric switch fixing seat 21, rotary driving block 22, thrust ball bearing 23, thrust ball bearing steel ball Cage fixing ring 24 , rotating detection table driving motor 25 , positioning bolt 26 of optical fiber detection head mounting seat, driving motor 27 of optical fiber detection head mounting seat, automatic sampling module driving motor 28 , and screw rod 29 .

detailed description

The present invention will be further described below in conjunction with the accompanying drawings, but the present invention is not limited to the following embodiments.

As shown in Figure 1, the present invention includes a fluorescence detection module, an optical fiber control module, an automatic sampling module, a rotation detection platform module, a motor control module and a host computer module, a fluorescence detection module, an optical fiber control module, an automatic sampling module, a rotation detection As shown in Figure 2, as the hardware part of the entire detection system, the motor control module is connected with the optical fiber control module, the automatic sampling module and the rotary detection platform module respectively, and the fluorescence detection module is connected with the rotary detection platform module. The optical fiber control module is connected, and the host computer module is respectively connected with the fluorescence detection module and the motor control module.

As the software part of the whole detection system, the upper computer module is connected with the motor control module and the fluorescence detection module respectively, and the hardware part is completed sequentially under the control of the software part. the entire testing process.

As shown in Figure 2 and Figure 3, the rotary detection platform module includes a disposable syringe fixed plate 8, a cylindrical connector 7, a disposable test tube fixed plate 6, a rotary detection platform positioning pin 19, a second photoelectric switch 20, a second photoelectric switch The fixed seat 21, the rotation drive block 22, the thrust ball bearing 23, the thrust ball bearing steel ball cage fixing ring 24 and the rotation detection table drive motor 25, the rotation detection table drive motor 25 is fixedly installed on the bottom surface of the middle part of the detection table base 1, and the rotation detection The output shaft of the table drive motor 25 passes through the through hole of the test table base 1 upwards and is coaxially fixedly connected with the square rotating drive block 22, and the rotating drive block 22 is set in the square hole at the center of the bottom surface of the disposable test tube fixing plate 6 , when the rotating detection table drive motor 25 is rotated, the main part of the rotating detection table can also rotate thereupon. A thrust ball bearing 23 is connected between the disposable test tube fixing plate 6 and the top surface of the detection table base 1, and the outer periphery of the thrust ball bearing 23 is fixed by a radial limit of the thrust ball bearing steel ball cage fixing ring 24; the disposable test tube fixing plate The top of 6 is evenly distributed vertically along the circumference with a plurality of disposable test tubes 18, and the center of the top of the disposable test tube fixing plate 6 is coaxially fixedly connected with the disposable syringe fixing plate 8 through the cylindrical connector 7, and the disposable test tube fixing plate 6 and Between the cylindrical connectors 7, between the cylindrical connectors 7 and the disposable syringe fixing plate 8, all are fixedly connected by bolts. Disposable syringe 17, the needle of disposable syringe 17 is inserted into the disposable test tube 18 downward; the second photoelectric switch 20 is located at one side of the disposable test tube fixing plate 6, and the second photoelectric switch 20 is fixed by the second photoelectric switch fixing seat 21 Installed on the detection table base 1, the rotating detection table positioning pin 19 is fixedly installed on the peripheral surface of the disposable test tube fixed disk 6 along the radial direction, and the rotating detection table positioning pin 19 cooperates with the second photoelectric switch 20; the optical fiber control module and the automatic The sample injection module is installed on the detection table base 1 on the side of the disposable test tube fixing plate 6 .

When detecting, the disposable test tube 18 is inserted into the uniformly distributed test tube mounting hole of the disposable test tube fixing plate 6 for positioning, and the disposable syringe 17 is inserted into the uniformly distributed syringe mounting hole of the disposable syringe fixing plate 8 for positioning. Equal and coaxial with the number of syringe mounting holes, ensure that the needle port of the disposable syringe 17 is inserted into the disposable syringe fixed plate 8 and insert the disposable test tube 18, so that the reagents in the disposable syringe are all injected into the disposable test tube 18. The disposable test tube fixing plate 6 and the disposable syringe fixing plate 8 have different numbers of mounting hole specifications, and different specifications are selected according to actual testing requirements. When the rotary control end of the rotary detection table rotates, the whole part of the rotary detection table rotates together. The positioning pin 19 of the rotation detection platform is used for initial positioning of the rotation detection platform before the detection starts. When the rotation detection platform positioning pin 19 triggers the second photoelectric switch 20 to respond during the rotation process, the signal is fed back to the motor control module and then controlled to stop the rotation detection platform drive motor 25, that is, the detection initialization is completed and the rotation detection platform reaches the initial position. When the axis of the optical fiber head intersects with the axis of the test tube, the distance is the shortest. During the detection process, each rotation of the rotary detection table driving motor 25 drives the rotary detection table to rotate 360/N° (wherein N is the number of holes that are currently evenly distributed), that is, the optical fiber 5 is kept in the normal state while keeping the optical fiber 5 still. The right samples to be tested are converted sequentially. The main part of the rotating detection table in the rotating detection table module and the rotating control end can be separated. The driving motor 25 of the rotating detection table can adopt a 42 two-phase stepping motor from Leisai Technology Co., Ltd., but it is not limited thereto.

As shown in Figure 2 and Figure 4, the automatic sampling module includes a linear guide rail 9, a lifting slider 10, a disposable syringe compression push plate 11, a lifting slider positioning trigger plate 12, a third photoelectric switch 13, and a third photoelectric switch fixed Disc 14, fourth photoelectric switch 16, fourth photoelectric switch fixed disc 15, automatic sampling module driving motor 28 and screw mandrel 29; automatic sampling module driving motor 28 is fixedly installed on the bottom surface of detection table base 1, and automatic sampling module drives The output shaft of the motor 28 passes through the through hole of the detection table base 1 and is coaxially fixedly connected with the screw rod 29. The side of the screw rod 29 is vertically and parallelly installed with a linear guide rail 9, and the screw rod 29 is covered with a lifting slider 10 embedded in it. The linear guide rail 9 moves up and down, and the side of the lifting slider 10 is fixedly connected with the lifting slider positioning trigger plate 12 and the disposable syringe compression pushing plate 11; the third photoelectric switch 13 and the fourth photoelectric switch 16 respectively pass through the third photoelectric switch. 14. The fourth photoelectric switch fixed disk 15 is fixedly installed on the frame of the detection table, and is located on the same side of the lifting slider positioning trigger plate 12. The lifting slider positioning trigger plate 12 is connected with the third photoelectric switch 13 and the fourth photoelectric switch 16. Coordination; the top of the disposable syringe compression pusher plate 11 is provided with an extension rod facing the rotary detection platform module, and the station directly below the extension rod is where the disposable syringe 17 is located, and the extension rod is used to squeeze the disposable syringe 17 .

When the driving motor 28 of the automatic sampling module is driven by the motor control module, the sliding guide rail is driven to rotate through the coupling, and then the disposable syringe is driven to compress the pushing plate 11 and the lifting slider positioning trigger plate 12 to move together in the vertical direction . When the disposable syringe compression pushing plate 11 touches the top of the disposable syringe 17 inserted on the disposable syringe mounting plate 8 during the descent process, it can push the piston of the disposable syringe 17 under the drive of the motor, and the disposable syringe 17 The liquid to be tested in is injected into the disposable test tube 18 directly below. While the lifting slider positioning trigger plate 12 is in the lifting process, the position in the vertical direction is always kept between the third photoelectric switch 13 and the fourth photoelectric switch 16 . When the lifting slider positioning trigger plate 12 moves downwards to trigger the fourth photoelectric switch 16 to respond, the motor control module receives the feedback signal and then controls the automatic sampling module drive motor 28 to stop rotating, that is, the lifting slider 10 stops moving downwards, once The syringe compresses the pushing plate 11 to stop the downward action; when the lifting slider positioning trigger plate 12 moves upward to trigger the third photoelectric switch 13, the same motor control module receives the feedback signal to control the automatic sampling module driving motor 28 to stop rotating, and the lifting slide Block 10 stops the upward movement. The specific auto-sampling module driving motor 28 can be a 42 two-phase stepping motor from Leisai Technology Co., Ltd., but it is not limited thereto.

As shown in Figure 2 and Figure 5, the optical fiber control module includes the first photoelectric switch 3, the first photoelectric switch fixing seat 2, the optical fiber detection head mounting seat 4, the optical fiber 5, the optical fiber detection head mounting seat positioning bolt 26 and the optical fiber detection head drive The motor 27, the optical fiber detection head drive motor 27 is fixedly installed on the bottom surface of the detection table base 1, the output shaft of the optical fiber detection head drive motor 27 passes through the through hole of the detection table base 1 and is coaxially fixedly connected with the optical fiber detection head mounting seat 4, The upper part of the optical fiber detection head mounting base 4 is horizontally installed with an optical fiber 5 through the optical fiber detection head mounting base 4, and the lower part of the optical fiber detection head mounting base 4 is horizontally installed with a positioning bolt 26 of the optical fiber detection head mounting base parallel to the optical fiber 5, and the first photoelectric switch 3 passes through The first photoelectric switch fixing base 2 is installed on the side of the optical fiber detection head installation base 4 , and the positioning bolt 26 of the optical fiber detection head installation base cooperates with the first photoelectric switch 3 .

When the optical fiber detection head driving motor 27 rotates, it drives the optical fiber detection head installation seat 4, and then drives the optical fiber 5 to rotate, so as to align with the disposable test tube 18 containing the liquid to be tested. In addition, when the optical fiber detection head driving motor 27 rotates, the positioning bolt 26 of the optical fiber detection head installation base also rotates accordingly. When the positioning bolt 26 of the optical fiber detection head mounting seat triggers the response of the first photoelectric switch 3 during rotation, the motor controller will receive real-time feedback and control the optical fiber detection head drive motor 27 to stop rotating, thereby realizing the adjustment of the optical fiber detection head mounting seat. 4 Positioning and initialization during rotation. The driving motor 27 of the optical fiber detection head in the specific implementation can adopt the 42 two-phase stepping motor of Leisai Technology Co., Ltd., but it is not limited thereto.

As shown in Figure 6, the fluorescence detection module includes a fluorescence excitation light source and a fluorescence detector, and the fluorescence excitation light source and the fluorescence detector are connected to the optical fiber 5 of the optical fiber control module. The optical fiber 5 of the optical fiber control module is a Y-shaped optical fiber, and the optical fiber 5 is bifurcated. The two ends are respectively connected to the fluorescence excitation light source and the fluorescence detector; when the positioning bolt 26 of the optical fiber detection head mounting seat is driven by the optical fiber detection head drive motor 27 to rotate to the detection port where the first photoelectric switch 3 is located, one end of the optical fiber 5 assembly is aligned and rotated The bottom of the disposable test tube 18 containing the liquid to be tested is placed on the detection platform module. The light emitted by the fluorescence excitation light source is transmitted through the optical fiber 5 to excite the quantum dots in the liquid to be tested contained in the disposable test tube 18 to generate fluorescence. Fluorescence is transmitted to a fluorescence detector through an optical fiber to obtain a fluorescence spectrum. Afterwards, the fluorescence detection module transmits the collected spectral data to the host computer module through a USB or UART interface, but not limited thereto. The fluorescence detector can be PMT, spectrometer or CCD, but not limited thereto.

As shown in Figure 7, the motor control module includes a motor controller and three motor drivers, the motor controller is connected to the three motor drivers, and the three motor drivers are respectively connected to the optical fiber control module of the present invention, the automatic sampling module and the rotation detection platform module The three motors in the motor are connected. The upper computer module controls the movement of the motor to control the three modules to perform corresponding actions. The motor controller outputs PWM pulses to the motor driver to control the motion state of the motor.

The motor controller communicates with the host computer module through a USB or UART interface, but is not limited thereto, and receives motor control commands. Then the motor controller outputs corresponding control pulses to the corresponding motor driver according to the command, and then drives the corresponding motors in the optical fiber control module, automatic sampling module, and rotary detection platform module to perform corresponding actions. The motor controller can be an Atmega 16A single-chip microcomputer of Atmel Company, and the motor driver can be a digital stepping motor driver DM422C of Raytheon Technology Company, but they are not limited thereto.

The number of disposable test tube fixing disks 6 and disposable syringe fixing disks 8 is the same, and different quantity specifications are adopted according to actual testing needs.

The upper computer module uses a computer to provide the control and analysis function of the entire system during detection, and communicates with the motor control module and the fluorescence detection module through USB or UART interfaces. At the same time, as a human-computer interaction interface, the experimenter can directly control the operation of the hardware part of the detection system, and finally complete the entire detection process.

As shown in Figure 8, the host computer module can process data based on the X86 computer hardware platform and the LabVIEW programming language, but is not limited thereto. At the beginning, it is necessary to make initial settings for the input of the experimenter, the experimental sample to be tested, and the fluorescence spectrum corresponding to the quantum dot-the concentration model of the sample to be tested, as well as the selection of manual or automatic detection mode during detection. The host computer module performs filtering, peak-finding and other processing on the obtained raw spectral data, and then calculates the detection results of the target objects in the sample to be tested based on this and the currently used model. Real-time display of the obtained original spectra, processed spectra and final detection results, and can save all the setting parameters, current detection environment and detection results involved in the entire detection process.

As shown in Figure 8, the specific implementation work process of the present invention is as follows:

1) Preparation of the test solution: Prepare the test solution according to the conventional detection method.

① Configure the solution of the test substance;

② Biochemically modify the quantum dots used so that they can have a better specific binding effect with the analyte;

③According to the detection requirements, take an appropriate amount of the test solution and mix it with the modified quantum dot solution and make them react and combine until a stable final solution of quantum dot-modifier-test object in the shape of a "sandwich" is formed;

2) According to the detection requirements, select the disposable test tube fixed plate 6 and the disposable syringe fixed plate 8 with the appropriate number of installation holes; open the detection table, take out the rotating detection table, and use several disposable syringes 17 to extract the liquid to be tested according to the current detection requirements , Then insert the disposable syringe fixed disk 8 and the disposable test tube fixed disk 6 respectively with the same number of disposable test tubes 18 and fix, then the rotating detection table is put back to its original position, after confirming that there is no skew, re-close the detection table. Turn on the power of the hardware part, so that the fluorescent excitation light source and other components enter the working state;

3) System initialization: the detection condition parameters such as fluorescence detection parameters are set in the upper computer module, and then the initialization signal is executed to return the hardware part to the initial state: the lifting slider positioning trigger plate 12 of the automatic sample injection module is located at the right position to trigger the third The photoelectric switch 13 position, the optical fiber detection head driving motor 27 of the optical fiber control module rotates until the positioning bolt 26 of the optical fiber detection head mounting seat triggers the first photoelectric switch 3 to respond, and the rotation detection table positioning pin 19 of the rotation detection table module rotates to trigger the second photoelectric switch. Switch 20 responds;

4) Spectrum detection: Perform spectrum detection on the sample to be tested currently at the detection position, turn on the excitation light source to irradiate the solution, excite the quantum dots to generate fluorescence, receive the generated fluorescence spectrum, and obtain spectral data;

5) Data processing and storage: the upper computer module performs filtering, peak-finding and other processing on the collected spectral data, and calculates the detection results according to the currently established model, and then displays and saves them;

6) Rotation to the next sample: After the detection of a portion of the liquid to be tested is completed, the rotating detection table module rotates at a certain angle driven by the rotating control end, so that the optical fiber 5 is aligned with the sample to be tested in the next disposable test tube 18 . Repeat steps 4 to 6 until a round of testing of all liquids to be tested is completed;

7) Check the data, deal with the aftermath, and end the measurement: the upper computer module executes the stop signal, and the motor control module controls the automatic sampling module and the rotary detection platform module to reach the stop state position. Then the detection platform is opened, the rotary detection platform is taken out, and the used disposable syringe 17 and disposable test tube 18 are discarded. After checking that there is no liquid contamination of the rotating detection table, put it back to the original position, and after confirming that there is no skew, close the detection table again. Turn off the power.

The data processing process of the upper computer module includes the cooperation process of the motor control module, fluorescence detection module, automatic sample injection module and upper computer module in the spectrum measurement in this detection system, as follows:

(1) The host computer module sends a start acquisition signal to the fluorescence detection module, controls the fluorescence detector to start collecting the fluorescence spectrum of the empty test tube, and then transmits it to the host computer module;

(2) The host computer module sends a stop collection signal to the fluorescence detection module, and controls the fluorescence detector to stop collection;

(3) The motor control module controls the work of the automatic sample injection module, injects the sample in the disposable syringe 17 into the current disposable test tube 18;

(4) The host computer module sends a start acquisition signal to the fluorescence detection module, controls the fluorescence detector to start collecting the fluorescence spectrum of the liquid to be tested, and then transmits it to the host computer module;

(5) The upper computer module sends a stop signal to the fluorescence detection module to control the fluorescence detector to stop collection.

The entire detection process is automated except for the configuration of the liquid to be tested and the steps of sampling and discarding.

Excluding the preparation time of the liquid to be tested, the detection time of a single sample includes complete steps such as spectrum acquisition and data processing, and the detection of a single station sample is controlled within 1 minute. For the whole round of detection, taking nine holes as an example, the total time of the implementation process can be controlled within 10 minutes, which not only realizes high-throughput and rapid detection, but also eliminates the cumbersome manual data processing in the later stage. It can be seen that the present invention The technical effect is prominent.

The specific embodiments above are used to explain the present invention, rather than to limit the present invention. Within the spirit of the present invention and the scope of protection of the claims, any modification and revision made to the present invention will fall within the protection scope of the present invention.

Claims (8)

1. A kind of 1. portable biochemical rapid detection system based on Quantum Dot Labeling, it is characterised in that：Including fluoroscopic examination Module, optical fiber control module, auto injection module, rotation monitor station module, motor control module and upper computer module, fluorescence inspection Module, optical fiber control module, auto injection module, rotation monitor station module and motor control module composition detection table apparatus are surveyed, Motor control module is connected with optical fiber control module, auto injection module and rotation monitor station module respectively, fluoroscopic examination module It is connected with optical fiber control module, upper computer module is connected with fluoroscopic examination module and motor control module respectively, upper computer module Using computer；

   Described rotation monitor station module includes disposable syringe fixed disk (8), cylindrical connection (7), disposable test tube and consolidated Price fixing (6), rotation monitor station alignment pin (19), the second optoelectronic switch (20), the second optoelectronic switch fixed seat (21), rotation driving Block (22), thrust ball bearing (23), thrust ball bearing ball retainer retainer ring (24) and rotation monitor station motor (25)；

   Rotation monitor station motor (25) is fixedly mounted on bottom surface in the middle part of monitor station base (1), rotates monitor station motor (25) output shaft is upward through the through hole of monitor station base (1) with being fixedly and coaxially connected in the rotation driving block (22) of cube, Rotation driving block (22) is sleeved in the square hole of disposable test tube fixing dish (6) bottom center, disposable test tube fixing dish (6) with Thrust ball bearing (23) is connected between monitor station base (1) top surface, thrust ball bearing (23) outside passes through thrust ball bearing Ball retainer retainer ring (24) radially spacing fixation；It is circumferentially vertical uniformly at intervals at the top of disposable test tube fixing dish (6) to fill There are more disposable test tubes (18), disposable test tube fixing dish (6) top center is through cylindrical connection (7) and disposable syringe Fixed disk (8) is fixedly and coaxially connected, on disposable syringe fixed disk (8) circumferentially it is vertical uniformly at intervals be equipped with it is each once Disposable syringe (17) corresponding to property test tube (18), disposable syringe (17) syringe needle are inserted downwardly into disposable test tube (18) In；

   Second optoelectronic switch (20) is located at the disposable side of test tube fixing dish (6) one, and the second optoelectronic switch (20) passes through the second light Electric switch fixed seat (21) is installed on monitor station base (1), and rotation monitor station alignment pin (19) is radially secure to be arranged on The side face of disposable test tube fixing dish (6) bottom, rotation monitor station alignment pin (19) coordinate with the second optoelectronic switch (20), optical fiber Control module and auto injection module are arranged on the monitor station base (1) of disposable test tube fixing dish (6) surrounding side.
2. 2. a kind of portable biochemical rapid detection system based on Quantum Dot Labeling according to claim 1, it is special Sign is：Described auto injection module includes line slideway (9), lifting slider (10), disposable syringe compression pushing plate (11), lifting slider orientation triggering plate (12), the 3rd optoelectronic switch (13), the 3rd optoelectronic switch fixed disk (14), the 4th photoelectricity Switch (16), the 4th optoelectronic switch fixed disk (15), auto injection module drive motor (28) and screw mandrel (29)；

   Auto injection module drive motor (28) is fixedly mounted on monitor station base (1) bottom surface, auto injection module drive motor (28) output shaft is upward through the through hole of monitor station base (1) and screw mandrel (29) is fixedly and coaxially connected, and screw mandrel (29) side is erected It is directly parallel that line slideway (9) is installed, it is cased with moving up and down in the embedded line slideway (9) of lifting slider (10) on screw mandrel (29), Lifting slider (10) side is fixedly connected with lifting slider orientation triggering plate (12) and disposable syringe compression pushing plate (11)； 3rd optoelectronic switch (13), the 4th optoelectronic switch (16) pass through the 3rd optoelectronic switch fixed disk (14), the 4th optoelectronic switch respectively Fixed disk (15) is fixedly mounted in monitor station frame, and is located at lifting slider orientation triggering plate (12) same side, and lifting is slided Block orientation triggering plate (12) coordinates with the 3rd optoelectronic switch (13) and the 4th optoelectronic switch (16)；Disposable syringe compression promotes Extension rod is provided with towards rotation monitor station module at the top of plate (11), is the work where disposable syringe (17) immediately below extension rod Position, extension rod are used to extrude disposable syringe (17).
3. 3. a kind of portable biochemical rapid detection system based on Quantum Dot Labeling according to claim 1, it is special Sign is：Described optical fiber control module includes the first optoelectronic switch (3), the first optoelectronic switch fixed seat (2), fiber laser arrays head Mounting seat (4), optical fiber (5), fiber laser arrays head mounting seat bolt (26) and fiber laser arrays head motor (27), optical fiber inspection Gauge head motor (27) is fixedly mounted on monitor station base (1) bottom surface, the output axial direction of fiber laser arrays head motor (27) On be fixedly and coaxially connected through the through hole of monitor station base (1) with fiber laser arrays head mounting seat (4), fiber laser arrays head mounting seat (4) top is horizontally installed with optical fiber (5), fiber laser arrays head mounting seat (4) lower horizontal peace by fiber laser arrays head mounting seat (4) Equipped with the fiber laser arrays head mounting seat bolt (26) parallel with optical fiber (5), the first optoelectronic switch (3) is opened by the first photoelectricity Close fixed seat (2) and be arranged on fiber laser arrays head mounting seat (4) side, fiber laser arrays head mounting seat bolt (26) and the first light Electric switch (3) coordinates.
4. 4. a kind of portable biochemical rapid detection system based on Quantum Dot Labeling according to claim 3, it is special Sign is：The is rotated to when the fiber laser arrays head mounting seat bolt (26) is driven by fiber laser arrays head motor (27) During detection mouth where one optoelectronic switch (3), the disposable test tube (18) in optical fiber (5) alignment rotation monitor station module.
5. 5. a kind of portable biochemical rapid detection system based on Quantum Dot Labeling according to claim 1, it is special Sign is：The fluoroscopic examination module includes fluorescence excitation light source, fluorescence detector, fluorescence excitation light source and fluorescence detector with Optical fiber (5) connection of optical fiber control module.
6. 6. a kind of portable biochemical rapid detection system based on Quantum Dot Labeling according to claim 5, it is special Sign is：The optical fiber (5) of the optical fiber control module is y-type optical fiber, and the both ends of optical fiber (5) bifurcated connect fluorescent exciting respectively Source and fluorescence detector, one end alignment of set rotate disposable test tube (18) bottom that prepare liquid is held in monitor station module.
7. 7. a kind of portable biochemical rapid detection system based on Quantum Dot Labeling according to claim 1, it is special Sign is：Described motor control module includes electric machine controller and three motor drivers, electric machine controller and three motors Driver is connected, three motor drivers respectively with described optical fiber control module, auto injection module and rotation monitor station mould Three motors in block are connected.
8. 8. a kind of portable biochemical rapid detection system based on Quantum Dot Labeling according to claim 7, it is special Sign is：Described electric machine controller exports pwm pulse to motor driver and then the motion state of controlled motor.