CN105176962A - Method for immobilizing bio-enzyme in ceramic filling material - Google Patents
Method for immobilizing bio-enzyme in ceramic filling material Download PDFInfo
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Abstract
The invention relates to a bio-enzyme immobilization method in the field of sewage treatment technology, specifically to a method for immobilizing bio-enzyme in a ceramic filling material. The method comprises the following steps: step 1, treatment of the bio-enzyme; step 2, treatment of the ceramic filling material; and step 3, mixing of the treated bio-enzyme and ceramic filling material so as to prepare a biological ceramic filling material. Through immobilization of the enzyme on the ceramic filling material, the effect of enzymatic reaction can be improved; so various pollutants in sewage is better removed, metabolites secreted by microbes are effectively decomposed, accumulation of sludge among and in the filling material is eliminated, unique permanent high efficiency of the enzymatic reaction is given to play, and the filling material is endowed with self-purifying and self-restoration capabilities and has prolonged service life.
Description
Technical field
The present invention relates to the method for a kind of bio-enzyme immobilization in sewage treatment process field, specifically refer to a kind of method be fixed on by biological enzyme in ceramic packing.
Background technology
A kind of novel sewage disposal technology started in late 1980s at biological aerated filter process, the feature that hydraulic load is large, hydraulic detention time is short owing to having, effluent quality is good, working cost is few etc. and receive increasing concern.And the essence of this technique is filling filler in biological reaction tank, microbial film is covered with at its filling surface and inside, by microbiological oxidation Decomposition in microbial film by the organic pollutant removal in sewage, for this reason filling surface and Internal biological film formation, come off and the speed that upgrades and quality, directly will affect decontamination effect improving.Compared with other microbial treatment methods, the application of zymotechnic has the advantages such as catalytic efficiency is high, reaction conditions is gentle, low for equipment requirements, speed of response is fast.Can say that zymotechnic application is the very potential method of one in the treatment of waste water, but enzyme is poor at high temperature, strong acid condition stability inferior, easy in inactivation, enzymic activity is vulnerable to the impact of Pollutants in Wastewater, can not reuse.Immobilized enzyme is exactly that people update the technology grown up in the process seeking to overcome these deficiencies.
Enzyme immobilizatio be with solid material by enzyme constraint or be limited in certain area, still can carry out its peculiar catalyzed reaction and a class technology that is recyclable and recycling, be exactly by chemistry or the treatment process of physics, original water miscible enzyme carrier insoluble with solid-state water combined or loaded body embeds.As a part for immobilized enzyme, the various performances of stuctures and properties to immobilized enzyme of solid support material have tremendous influence.Immobilized enzyme is not only in the research Showed Very Brisk of the ambits such as chemistry, biology and biotechnology, medical science and life science; obtain developing rapidly and apply widely, and because there is saving resource and the energy, reduce environmental pollution and meet the strategic requirement of Sustainable development.
Ceramic packing (porcelain ring) has excellent acidproof heat-proof performance, the various acid of ability except the acid of fluorine oxygen, the corrosion of alkali.Ceramic packing can be used for the liner of the washing tower of the industries such as chemical industry, metallurgy, relieving haperacidity, coal gas, oxygen, iron and steel, pharmacy, fine chemistry industry, cooling tower, reclaiming tower, thionizer, drying tower, absorption tower and reactor.It is made up of many filler unit bodies with same geometry.Due to the structure that pottery is unique, there is good hydrophilicity, the tiled serpentine passage capable of being that its surface can form the turbulence of very thin liquid film and air-flow promotes air-flow but barrier air does not make ceramic packing can be equal to mutually with metallic stuffing, and it is corrosion-resistant, resistance to elevated temperatures metallic stuffing cannot be compared.Surface tissue has good wet performance, can make that liquid accelerates flowing, that filler dispersed phase holdup is down to is minimum.Thus reduce overheated, polymerization and the chance of coking.
As the ceramic packing with " activity ", following condition must be possessed:
1) first require that there is larger specific surface area, for microbial growth and breeding provide necessary place.
2) require filler structure and space be conducive to promoting water sports, increasing the contact area of dirty Organic substance in water and filler, raising effect of mass transmitting.
3) there is easily attachment and fixing carrier, continue to carry out enzymatic reaction on the surface of filler and inside, keep microbic activity.
Have the research about enzyme immobilization and biologic packing material, relevant report has [Zhang Weiwei, " master thesis ", the enzyme immobilization research of biological carbon fiber, 20100604]; Chinese patent CN200510035661.9 discloses a kind of porous ceramics with saprobia flora, include in the hole of porous ceramics and can to carry out a biological disposal upon to sewage and through cryodrying process, the flora being in dormant state, beneficial microorganism can be made in use to breed with the fastest speed, thus accelerate the purifying treatment speed of sewage, improve wastewater treatment efficiency.But the method can not be recycled and easily lose efficacy, and is not suitable for industrial production.Chinese patent CN1314600C " modified microbe stuffing and preparation method thereof ", this invention high density polyethylene(HDPE), ground rubber, light calcium carbonate, crust poison, carbon black is mixed to join acetone mixing solutions, after allowing acetone naturally volatilize, drop in screw extrusion press, melt extrude shaping.Owing to using maleic anhydride, the chemical agent character of dicumyl peroxide and acetone, reduces the affinity of material quality to microorganism, simultaneously after melt extruded, destroys the micro hole structure of material internal.
How to select a kind of productive expense cheap, applied widely, operate the simple and easy and enzyme immobilization technique that can recycle, just become a kind of widespread consensus of current enzyme immobilization research.And how effectively to realize biological enzyme to be fixed in ceramic packing, also just become a study hotspot at present.
Summary of the invention
The object of the invention is in order to the weak point for existing ceramic packing, a kind of method effectively realizing being fixed on by biological enzyme in ceramic packing is provided, and there is actual operability, the obvious feature of industrial application value.
The invention provides a kind of method be fixed on by biological enzyme in ceramic packing, the method comprises:
Step one, the process that processes that biological enzyme is carried out;
Step 2, the process that processes that ceramic packing is carried out;
Step 3, by the process of the biological enzyme after process and the obtained bioceramic filler of ceramic packing mixing;
Described step one comprises: by NaOH solution, the pH of distilled water is transferred to 8.0 ~ 8.5, and adds hydrogen peroxide, and making to contain mass content in the NaOH aqueous solution is the hydrogen peroxide of 0.06%, and the NaOH aqueous solution is heated to 30 ~ 35 DEG C;
By the biological enzyme chosen with 1: 5 mass ratio be scattered in the above-mentioned NaOH aqueous solution, shake 20 minutes by frequency 40 ~ 50K hertz ultrasonic wave, then carry out centrifugal 15 minutes with 5000r/min, get supernatant liquor; Add the borate buffer solution dissolution precipitation that pH is the 0.2mol/L of 9.0, after elimination insolubles, obtain basic protein modification enzyme liquid, for subsequent use in the refrigerator leaving temperature 4 DEG C in;
Described step 2 comprises: by ceramic packing vibration screening ash disposal dedusting, add properties-correcting agent, the weight ratio of described ceramic packing weight and properties-correcting agent is 3:1, described properties-correcting agent is comprise the glucosamine of 1% ~ 5%, corn steep liquor, 1% ~ 5% benzoyl peroxide of 5% ~ 15% by weight percentage, the ferric sulfate of 1% ~ 2%, the peptone of 5% ~ 20%, 53% ~ 87% ethyl acetate mixture;
Described step 3 comprises in the ceramic packing obtained to step 2 the basic protein modification enzyme liquid added obtained by step one, and leave standstill after fully stirring, the described add-on of basic protein modification enzyme liquid and the mass ratio of ceramic packing are 1:100-500; After using negative-pressure vacuum pump to vacuumize, air seasoning, finally makes the biological enzyme ceramic packing that water content is 8-10%.
Preferably, the NaOH solution used in the art breading in the described method be fixed on by biological enzyme in ceramic packing, hydrogen peroxide, glucosamine, corn steep liquor, peptone, benzoyl peroxide, ferric sulfate, ethyl acetate are analytical reagent.
Preferably, in described method biological enzyme is fixed in ceramic packing in step one for the pH of distilled water is transferred to 8.0 ~ 8.5 with 0.5MNaOH, and add 30% hydrogen peroxide, and to make in the NaOH aqueous solution containing mass content to be the hydrogen peroxide of 0.06%.
Preferably, borate buffer solution preparation method in the described method be fixed on by biological enzyme in ceramic packing in step one is: get boric acid and be dissolved in the water, and then regulate pH to 9.0 with 40% sodium hydroxide solution, and strength of solution is made to be the borate buffer solution of 0.2mol/L.
Preferably, in the described method be fixed on by biological enzyme in ceramic packing, the specific surface area that should meet of the ceramic packing of step 2 is greater than 200m
2/ g, particle diameter are 10 ~ 20mm, and voidage is greater than 95%.
Preferably, the feed postition adding properties-correcting agent in the described method steps two be fixed on by biological enzyme in ceramic packing is the mode of spraying, and spray time is 15 ~ 20 minutes, stirs filler in the process of spraying.
Preferably, the basic protein modification enzyme liquid obtained by step one is added in the ceramic packing obtained to step 2 in step 3 in the described method be fixed on by biological enzyme in ceramic packing, abundant stirring leaves standstill 15 ~ 30 minutes afterwards, the pressure of vacuum pump is 65 ~ 75KPa, pumpdown time is 20 ~ 40 minutes, and drying temperature is less than 45 degree.
Preferably, described method biological enzyme is fixed in ceramic packing, the method comprises:
Step one, the process that processes that biological enzyme is carried out;
Step 2, the process that processes that ceramic packing is carried out;
Step 3, by the process of the biological enzyme after process and the obtained bioceramic filler of ceramic packing mixing;
Described step one comprises: by the NaOH solution of 0.5M, the pH of distilled water is transferred to 8.3, and adds the hydrogen peroxide of 2ml30%, and making to contain mass content in the NaOH aqueous solution is the hydrogen peroxide of 0.06%, and the NaOH aqueous solution is heated to 35 DEG C;
By the biological enzyme chosen with 1: 5 mass ratio be scattered in the above-mentioned NaOH aqueous solution, shake 20 minutes by frequency 50K hertz ultrasonic wave, then carry out centrifugal 15 minutes with 5000r/min, get supernatant liquor; Add the borate buffer solution dissolution precipitation that pH is the 0.2mol/L of 9.0, after elimination insolubles, obtain basic protein modification enzyme liquid, for subsequent use in the refrigerator leaving temperature 4 DEG C in;
Described step 2 comprises: by ceramic packing vibration screening ash disposal dedusting, add properties-correcting agent, the weight ratio of described ceramic packing weight and properties-correcting agent is 3:1, add while seethe 15 minutes, then 15 minutes are left standstill, described properties-correcting agent is comprise the glucosamine of 5%, corn steep liquor, 5% benzoyl peroxide of 10% by weight percentage, the ferric sulfate of 1%, the peptone of 20%, 59% ethyl acetate mixture;
Described step 3 comprises in the ceramic packing obtained to step 2 the basic protein modification enzyme liquid added obtained by step one, and leave standstill 15 minutes after fully stirring, the described add-on of basic protein modification enzyme liquid and the mass ratio of ceramic packing are 1:150; After using 75KPa negative-pressure vacuum pump to vacuumize for 30 minutes, air seasoning, finally makes the biological enzyme ceramic packing that water content is 8%.
Preferably, the biological enzyme in the described method be fixed on by biological enzyme in ceramic packing is dry commercially available biological enzyme.
Acting as of properties-correcting agent described in the present invention: modification is carried out to ceramic packing surface and inside, removes ceramic surface negative charge and the nutritive substance of microorganism series and the immobilization carrier of biological enzyme are provided.Create condition for microorganism adheres to breeding on ceramic packing, the trace element needed by the microbial reproduction provided in properties-correcting agent and active factor composition, shorten the time of acclimated microorganism in early stage.By enzyme immobilization technology on ceramic packing, the effect of enzymatic reaction can be strengthened, except improving the removal to the various pollutent of sewage, effectively can also decompose the meta-bolites of microorganism secretion, eliminate the accumulation of the inner mud with filler between filler, play the distinctive permanent efficient performance of enzymatic reaction simultaneously, allow filler have the ability of oneself's purification self, extend filler work-ing life.
Beneficial effect of the present invention is that microbic activity is high, and has the ability of self, can life-time service.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, and following examples are explanation of the invention and the present invention is not limited to following examples.
Embodiment 1
Basic protein modification enzyme liquid makes
Get distilled water 1 liter, with 0.5MNaOH, pH is transferred to 8.3, add 2 milliliter of 30% hydrogen peroxide, water is heated to 35 DEG C.Taking 200g biological enzyme is scattered in the above-mentioned aqueous solution, shake 20 minutes by frequency 50K hertz ultrasonic wave, then centrifugal 15 minutes of 5000r/min, take out supernatant liquor 600ml, add the borate buffer solution 2ml dissolution precipitation that pH is the 0.2mol/L of 9.0, basic protein modification enzyme liquid 500ml is obtained after elimination insolubles
Prepared by properties-correcting agent
By weight percentage: the glucosamine of 5%, the corn steep liquor of 10%, 5% benzoyl peroxide, the ferric sulfate of 1%, the peptone of 20%, 59% ethyl acetate mixing for standby use.
Ceramic packing fixed biologically enzyme
Be 10mm the particle diameter through vibration screening ash disposal dedusting, specific surface area is 300m
2/ g ceramic packing 900g, adds properties-correcting agent 300g in the mode of spraying, stirs filler in spray process, and the time of stirring is 15 minutes, adds step 1 after static 30 minutes) in made basic protein modification enzyme liquid 9g after fully stirring static 15 minutes; After using negative-pressure vacuum pump (75KPa) to vacuumize for 30 minutes, air seasoning, finally makes the biological enzyme ceramic packing that water content is 8%.
Implementation step 1
Get the equalizing tank water outlet (water-quality COD of 20L printing and dyeing mill
cr2500mg/L-3800mg/L, pH8.2), add PAF and carry out coagulating sedimentation, result shows when the PAF adding 2000ppm concentration, and water-quality guideline is best, continues to add, and indices declines not obvious.Supernatant C OD
crbe down to 1200mg/L-1800mg/L, clearance is about 48%.
Implementation step 2
Get previously processed dyeing and printing sewage supernatant liquor 1L, join in ultrasonic predissolve pond, add 5.0g immobilization biological enzyme filler simultaneously, interval is carried out a ultrasonic-microwave for 10 minutes and is dissolved, after 1 hour, whole sewage samples of mixing are joined in laboratory room small-sized biochemical treatment apparatus, add the sewage supernatant liquor that 4L step 1 is handled well simultaneously again, open aerobic aeration.Effect as a comparison, under identical experiment condition, has done simultaneously and has added without the aerobic aeration of the ceramic packing of bio-enzyme immobilization and the aerobic aeration without immobilized biological enzyme.Aeration time is 30 minutes.Detect supernatant C OD
crindex, result is as shown in table 1.
CODcr detection method: dichromate titration
The visible ceramic packing adding immobilization biological enzyme, with compared with immobilized biological enzyme, improves 8% to the removal efficiency of COD.Analyze from cost, adding of immobilization biological enzyme makes the processing cost of water per ton increase only 0.1 yuan.Microbe population on biological enzyme ceramic packing reaches 2.5 × 10
10/ g, this filler uses after 2 months and still remains on more than 90% to the clearance of various contamination index.
In order to more directly contrast the action effect of immobilization biological enzyme, immobilization biological enzyme and the respiration rate situation producing microorganism without immobilized biological enzyme separately be have also been made comparative analysis by us: respectively get the immobilization biological enzyme of equivalent and be placed in culturing bottle respectively without immobilized biological enzyme, be filled with the dissolved oxygen of equivalent to the inside simultaneously, close envelope, after 5 days, measure the decline situation of dissolved oxygen in bottle with dissolved oxygen meter.As shown in the table:
Visible in the identical unit time, the oxygen amount of the microbial consumption that immobilization biological enzyme produces will far away higher than without immobilized biological enzyme, this just illustrates immobilized biological enzyme, its stability and activity, far away higher than without immobilized biological enzyme.
In the present embodiment, the NaOH adopted, hydrogen peroxide are analytical reagent.Borate buffer solution preparation method is: get boric acid and be dissolved in the water, and then regulates pH to 9.0 with 40% sodium hydroxide solution, and makes strength of solution be the borate buffer solution of 0.2mol/L.
Embodiment 2
Basic protein modification enzyme liquid makes
Get distilled water 1 liter, with 0.5MNaOH, pH is transferred to 8.5, add 2 milliliter of 30% hydrogen peroxide, water is heated to 30 DEG C.Taking 200g biological enzyme is scattered in the above-mentioned aqueous solution, shake 20 minutes by frequency 40K hertz ultrasonic wave, then centrifugal 15 minutes of 5000r/min, take out supernatant liquor 600ml, add the borate buffer solution 2ml dissolution precipitation that pH is the 0.2mol/L of 9.0, basic protein modification enzyme liquid 500ml is obtained after elimination insolubles
Prepared by properties-correcting agent
By weight percentage: the glucosamine of 3%, the corn steep liquor of 12%, 4% benzoyl peroxide, the ferric sulfate of 2%, the peptone of 18%, 61% ethyl acetate mixing for standby use.
Ceramic packing fixed biologically enzyme
Be 10mm the particle diameter through vibration screening ash disposal dedusting, specific surface area is 300m
2/ g ceramic packing 900g, adds properties-correcting agent 300g in the mode of spraying, stirs filler in spray process, and the time of stirring is 15 minutes, adds step 1 after static 30 minutes) in made basic protein modification enzyme liquid 9g after fully stirring static 15 minutes; After using negative-pressure vacuum pump (75KPa) to vacuumize for 30 minutes, air seasoning, finally makes the biological enzyme ceramic packing that water content is 8%.
Implementation step 1
Get the equalizing tank water outlet (water-quality COD of 20L printing and dyeing mill
cr2500mg/L-3800mg/L, pH8.2), add PAF and carry out coagulating sedimentation, result shows when the PAF adding 2000ppm concentration, and water-quality guideline is best, continues to add, and indices declines not obvious.Supernatant C OD
crbe down to 1200mg/L-1800mg/L, clearance is about 48%.
Implementation step 2
Get previously processed dyeing and printing sewage supernatant liquor 1L, join in ultrasonic predissolve pond, add 5.0g immobilization biological enzyme filler simultaneously, interval is carried out a ultrasonic-microwave for 10 minutes and is dissolved, after 1 hour, whole sewage samples of mixing are joined in laboratory room small-sized biochemical treatment apparatus, add the sewage supernatant liquor that 4L step 1 is handled well simultaneously again, open aerobic aeration.Effect as a comparison, under identical experiment condition, has done simultaneously and has added without the aerobic aeration of the ceramic packing of bio-enzyme immobilization and the aerobic aeration without immobilized biological enzyme.Aeration time is 30 minutes.Detect supernatant C OD
crindex, result is as shown in table 2.
CODcr detection method: dichromate titration
The visible ceramic packing adding immobilization biological enzyme, with compared with immobilized biological enzyme, improves 8% to the removal efficiency of COD.Analyze from cost, adding of immobilization biological enzyme makes the processing cost of water per ton increase only 0.1 yuan.Microbe population on biological enzyme ceramic packing reaches 2.5 × 10
10/ g, this filler uses after 2 months and still remains on more than 90% to the clearance of various contamination index.
In order to more directly contrast the action effect of immobilization biological enzyme, immobilization biological enzyme and the respiration rate situation producing microorganism without immobilized biological enzyme separately be have also been made comparative analysis by us: respectively get the immobilization biological enzyme of equivalent and be placed in culturing bottle respectively without immobilized biological enzyme, be filled with the dissolved oxygen of equivalent to the inside simultaneously, close envelope, after 5 days, measure the decline situation of dissolved oxygen in bottle with dissolved oxygen meter.As shown in the table:
Visible in the identical unit time, the oxygen amount of the microbial consumption that immobilization biological enzyme produces will far away higher than without immobilized biological enzyme, this just illustrates immobilized biological enzyme, its stability and activity, far away higher than without immobilized biological enzyme.
In the present embodiment, the NaOH adopted, hydrogen peroxide are analytical reagent.Borate buffer solution preparation method is: get boric acid and be dissolved in the water, and then regulates pH to 9.0 with 40% sodium hydroxide solution, and makes strength of solution be the borate buffer solution of 0.2mol/L.
In addition, it should be noted that all equivalences of doing according to the principle described in inventional idea of the present invention or simple change, be included in the protection domain of patent of the present invention.Those skilled in the art can make various amendment or supplement or adopt similar mode to substitute to described specific embodiment; only otherwise depart from structure of the present invention or surmount this scope as defined in the claims, protection scope of the present invention all should be belonged to.
Claims (9)
1. biological enzyme is fixed on the method in ceramic packing, it is characterized in that the method comprises:
Step one, the process that processes that biological enzyme is carried out;
Step 2, the process that processes that ceramic packing is carried out;
Step 3, by the process of the biological enzyme after process and the obtained bioceramic filler of ceramic packing mixing;
Described step one comprises: by NaOH solution, the pH of distilled water is transferred to 8.0 ~ 8.5, and adds hydrogen peroxide, and making to contain mass content in the NaOH aqueous solution is the hydrogen peroxide of 0.06%, and the NaOH aqueous solution is heated to 30 ~ 35 DEG C;
By the biological enzyme chosen with 1: 5 mass ratio be scattered in the above-mentioned NaOH aqueous solution, shake 20 minutes by frequency 40 ~ 50K hertz ultrasonic wave, then carry out centrifugal 15 minutes with 5000r/min, get supernatant liquor; Add the borate buffer solution dissolution precipitation that pH is the 0.2mol/L of 9.0, after elimination insolubles, obtain basic protein modification enzyme liquid, for subsequent use in the refrigerator leaving temperature 4 DEG C in;
Described step 2 comprises: by ceramic packing vibration screening ash disposal dedusting, add properties-correcting agent, the weight ratio of described ceramic packing weight and properties-correcting agent is 3:1, described properties-correcting agent is comprise the glucosamine of 1% ~ 5%, corn steep liquor, 1% ~ 5% benzoyl peroxide of 5% ~ 15% by weight percentage, the ferric sulfate of 1% ~ 2%, the peptone of 5% ~ 20%, 53% ~ 87% ethyl acetate mixture;
Described step 3 comprises in the ceramic packing obtained to step 2 the basic protein modification enzyme liquid added obtained by step one, and leave standstill after fully stirring, the described add-on of basic protein modification enzyme liquid and the mass ratio of ceramic packing are 1:100-500; After using negative-pressure vacuum pump to vacuumize, air seasoning, finally makes the biological enzyme ceramic packing that water content is 8 ~ 10%.
2. the method be fixed on by biological enzyme in ceramic packing according to claim 1, the NaOH solution that it is characterized in that using in above-mentioned art breading, hydrogen peroxide, glucosamine, corn steep liquor, peptone, benzoyl peroxide, ferric sulfate, ethyl acetate are analytical reagent.
3. method biological enzyme is fixed in ceramic packing according to claim 1, it is characterized in that in described step one for the pH of distilled water is transferred to 8.0-8.5 with 0.5MNaOH, and add 30% hydrogen peroxide, and make in the NaOH aqueous solution containing mass content be the hydrogen peroxide of 0.06%.
4. method biological enzyme is fixed in ceramic packing according to claim 1, it is characterized in that the borate buffer solution preparation method in described step one is: get boric acid and be dissolved in the water, and then regulate pH to 9.0 with 40% sodium hydroxide solution, and strength of solution is made to be the borate buffer solution of 0.2mol/L.
5. the method be fixed on by biological enzyme in ceramic packing according to claim 1, is characterized in that the specific surface area that should meet of the ceramic packing in described step 2 is greater than 200m
2/ g, particle diameter are 10 ~ 20mm, and voidage is greater than 95%.
6. the method be fixed on by biological enzyme in ceramic packing according to claim 1, it is characterized in that the feed postition adding properties-correcting agent in described step 2 is the mode of spraying, spray time is 15 ~ 20 minutes, stirs filler in the process of spraying.
7. method biological enzyme is fixed in ceramic packing according to claim 1, it is characterized in that the basic protein modification enzyme liquid added in the ceramic packing obtained to step 2 in described step 3 obtained by step one, abundant stirring leaves standstill 15 ~ 30 minutes afterwards, the pressure of vacuum pump is 65 ~ 75KPa, pumpdown time is 20 ~ 40 minutes, and drying temperature is less than 45 degree.
8. the method be fixed on by biological enzyme in ceramic packing according to claim 1, is characterized in that the method comprises:
Step one, the process that processes that biological enzyme is carried out;
Step 2, the process that processes that ceramic packing is carried out;
Step 3, by the process of the biological enzyme after process and the obtained bioceramic filler of ceramic packing mixing;
Described step one comprises: by the NaOH solution of 0.5M, the pH of distilled water is transferred to 8.3, and adds the hydrogen peroxide of 2ml30%, and making to contain mass content in the NaOH aqueous solution is the hydrogen peroxide of 0.06%, and the NaOH aqueous solution is heated to 35 DEG C;
By the biological enzyme chosen with 1: 5 mass ratio be scattered in the above-mentioned NaOH aqueous solution, shake 20 minutes by frequency 50K hertz ultrasonic wave, then carry out centrifugal 15 minutes with 5000r/min, get supernatant liquor; Add the borate buffer solution dissolution precipitation that pH is the 0.2mol/L of 9.0, after elimination insolubles, obtain basic protein modification enzyme liquid, for subsequent use in the refrigerator leaving temperature 4 DEG C in;
Described step 2 comprises: by ceramic packing vibration screening ash disposal dedusting, add properties-correcting agent, the weight ratio of described ceramic packing weight and properties-correcting agent is 3:1, add while seethe 15 minutes, then 15 minutes are left standstill, described properties-correcting agent is comprise the glucosamine of 5%, corn steep liquor, 5% benzoyl peroxide of 10% by weight percentage, the ferric sulfate of 1%, the peptone of 20%, 59% ethyl acetate mixture;
Described step 3 comprises in the ceramic packing obtained to step 2 the basic protein modification enzyme liquid added obtained by step one, and leave standstill 15 minutes after fully stirring, the described add-on of basic protein modification enzyme liquid and the mass ratio of ceramic packing are 1:150; After using 75KPa negative-pressure vacuum pump to vacuumize for 30 minutes, air seasoning, finally makes the biological enzyme ceramic packing that water content is 8%.
9., according to the method be fixed on by biological enzyme in ceramic packing described in claim 1 to 8, it is characterized in that described biological enzyme is dry commercially available biological enzyme.
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| CN109368811A (en) * | 2018-11-19 | 2019-02-22 | 北京正和恒基滨水生态环境治理股份有限公司 | Microbial compound enzyme filler and preparation method thereof and application method |
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