CN105169203A - Fructus amomi extract and application thereof - Google Patents

Abstract

The invention provides application of an aqueous fructus amomi extract and olatile oil in preparation of a drug for losing weight and reducing blood fat. Fructus amomi is smashed, soaked and decocted to obtain an aqueous extract, or the fructus amomi (Amomun villosum Lour) is smashed and soaked, and the volatile oil is extracted and collected through a steam distillation method. The obtained anti-obesity drug adopts raw materials with homology of medicine and food, has the effects of controlling the obesity induced by high fat diet, relieving hyperlipidaemia in an obesity state, inhibiting the high serum endotoxin state in the obesity state and inhibiting pancreatic lipase and can well achieve the purpose of healthily reducing the fat.

Description

A kind of amomum extract and its use

technical field

The invention relates to the field of medicines and health care products, in particular to an amomum extract and its application.

Background technique

Obesity (obesity) refers to a multifactorial chronic disease that occurs when the body eats more calories than it consumes, and the excess calories are stored in the body in the form of fat, resulting in excessive accumulation and/or abnormal distribution of body fat, which in turn leads to weight gain. metabolic disease. According to WHO statistics, there are now more than 300 million obese people in the world, and 1.1 billion people are overweight. The number of obese patients in China has exceeded 70 million, ranking 10th in the list of obesity incidence in the world. Relevant experts predict that in the next 10 years, the number of obese people in my country may exceed 200 million. As we all know, obesity is closely related to the occurrence and development of diseases such as cardiovascular and cerebrovascular diseases, hypertension, type 2 diabetes, dyslipidemia, and sleep apnea syndrome. What's more serious is that obesity can cause secondary diseases of other organs such as the heart, blood vessels, and lungs, and can shorten life expectancy. According to surveys, the average life expectancy of obese people is significantly lower than that of normal weight people. Therefore, obesity is rated as a health risk factor and poses a serious threat to human health and social development. Research and development of corresponding prevention and treatment methods have become an urgent need. However, the weight loss products commonly used in the market are mainly appetite control products, laxatives, diuretics, and metabolism-enhancing products. Although the obesity problem has been solved in a short period of time, many products contain harmful ingredients, which are actually even more harmful to human health. . Therefore urgently hope to obtain a kind of weight loss product with excellent effect.

Amomi Fructus, as one of the "four major southern medicines" in my country, has a medicinal and edible history of more than 1300 years. Amomum is a perennial herb of the genus Cardamom in the ginger family. It is warm in nature and pungent in taste. It has the functions of reducing dampness and appetizing, warming the spleen and stopping diarrhea, regulating qi and preventing miscarriage. It is a commonly used medicine for treating gastrointestinal diseases in traditional Chinese medicine. Modern pharmacological studies have shown that the main active ingredients in amomum include borneol acetate, camphor, limonene, borneol and other volatile substances, which can improve gastrointestinal function, relieve pain, stop diarrhea, promote the secretion of digestive juice, reduce blood lipids, and regulate bowel movements. Tract flora etc. As a commonly used medicinal and edible medicinal material, many years of clinical practice and modern research have shown that the medicinal material has the advantages of less toxic and side effects and significant efficacy.

At present, many weight loss products have the problems of large side effects and many adverse reactions. Therefore, we hope to provide a pure natural weight loss product with less toxic side effects and excellent weight loss effect in view of the above problems.

Contents of the invention

The purpose of the present invention is to overcome the problems of large side effects and many adverse reactions of many current weight loss products, and provide a pure natural weight loss product Amomum water extract and Amomum volatile oil with less toxic side effects and excellent weight loss effect.

Another object of the present invention is to provide a method for preparing the above slimming product.

The technical scheme that the present invention solves this technical problem is:

The preparation method of Amomum water extract comprises the following steps: crush Amomum and accurately weigh 50-150g, add 250-1000ml of distilled water to soak for half an hour, boil and decoct with low heat for 5-10 minutes, filter the medicinal liquid to obtain filtrate A and Medicinal residue A; medicinal residue A is decocted with distilled water for 5-10 minutes, and the liquid medicine is filtered to obtain filtrate B and medicinal residue B; medicinal residue B is added with distilled water and decocted for 5-10 minutes, and the medicinal liquid is filtered to obtain filtrate C; The filtrates A, B, and C were combined, concentrated, and freeze-dried to obtain the water extract of Amomum japonicus.

The preparation method of Amomum volatile oil comprises the following steps: accurately weigh 50-150g of amomum and place it in a round-bottomed flask, add 200-800ml of distilled water, soak for 0.5-1 hour, extract by steam distillation for 5-10h, Collect the volatile oil, which is Amomum volatile oil.

The invention also provides a preparation containing the Amomum extract, which consists of the Amomum extract and a pharmaceutically acceptable carrier.

The preparation is tablet, capsule or granule, oral liquid and injection.

The pharmaceutically acceptable carrier refers to a common drug carrier in the field of pharmacy, selected from surfactants, fillers, suspending agents, solvents, flavoring agents, binders, disintegrating agents, wetting agents or lubricants one or more of them.

The filler is selected from sucrose, dextrin, lactose, mannitol, starch, xylitol, glucose, etc.;

The binder is selected from alginate, starch, dextrin, gelatin or polyvinylpyrrolidone, etc.;

The disintegrating agent is selected from sodium hydroxymethyl starch, low-substituted hydroxypropyl cellulose, microcrystalline cellulose or sodium hydroxymethyl cellulose;

Described lubricant is selected from polyethylene glycol, sodium bicarbonate, micropowder silica gel, stearic acid or magnesium stearate;

The suspending agent is selected from cellulose, beeswax, solid polyethylene glycol or micropowder silica gel;

The wetting agent is selected from Tween-80, glycerin, lecithin;

The solvent is selected from glycerin, propylene glycol, vegetable oil, liquid polyethylene glycol or ethanol;

The surfactant is selected from stearic acid, sodium eicosylbenzenesulfonate, polysorbate or fatty acid sorbitan;

The flavoring agent is selected from sucrose, essence, citric acid, sodium saccharin or aspartame.

Beneficial effects of the present invention:

The weight-loss medicine uses raw materials of the same origin as medicine and food, and has the functions of controlling obesity induced by high-fat diet, alleviating hyperlipidemia in obese state, inhibiting high serum endotoxin state in obese state, and inhibiting pancreatic lipase. Good for healthy weight loss.

Description of drawings

Figure 1 Effect of different extracts of amomum on body weight

Among them, Golden Bifid: GoldenBifid (GLB)

Amomum volatile oil: VolatileOildfromFructusAmomi (VOA)

Amomum water extract: WaterextractfromFructusAmomi (WEA)

(VOA.H) High dose Amomum volatile oil

(VOA.M) Medium dose Amomum volatile oil

(VOA.L)Low dosage Amomum volatile oil

(WEA.H) High Dose Amomum Water Extract

(WEA.M) Medium Dose Amomum Water Extract

(WEA.L) Low Dose Amomum Water Extract

Figure 2 The effect of different extracts of amomum on the ratio of food intake to body weight

Figure 3 The effect of different extracts of Amomum on triglyceride (TG)

Figure 4 The effect of different extracts of amomum on total cholesterol (TC)

Fig. 5 Effect of different extracts of Amomum on endotoxin (LPS)

Figure 6 Effect of different extracts of Amomum on pancreatic lipase activity in vitro

Compared with normal group, ^* p<0.05, ^** p<0.01, ^*** p<0.001

^# p<0.05, ^## p<0.01, ^### p<0.001 compared to the model group

Detailed ways

Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the following examples, usually follow the conventional conditions or the conditions suggested by the manufacturer. All percentages, ratios, proportions or parts are by weight unless otherwise indicated.

Unless otherwise defined, all professional and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. In addition, any methods and materials similar or equivalent to those described can be applied to the method of the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only.

Embodiment 1: Preparation of amomum aqueous extract granules

1. Extraction method: Select Amomunvillosum Lour from Yunnan, crush it and weigh 200g, add 1000ml of distilled water to soak for half an hour, boil it, fry it with low heat for 10 minutes, and filter the liquid medicine. Add distilled water to the dregs of the medicine and decoct for 10 minutes, filter the liquid medicine, add distilled water to the dregs of the medicine and decoct for 5 minutes, combine the filtrates three times, concentrate, and freeze-dry, which is the water extract of Amomum japonicus.

2. Preparation: 50g of water extract of amomum seed, about 110g of starch, about 10g of essence, mixed evenly in equal amounts, granulated and subpackaged to obtain granules, 5g/bag, about 30 bags.

Embodiment 2: the preparation of amomum volatile oil granule

1 Extraction method: Select Amomunvillosum Lour from Yunnan, crush it, weigh 200g precisely, put it in a round bottom flask, add 1200ml of distilled water, soak for 1 hour, extract by steam distillation for 10h, and collect the volatile oil.

2 Preparation: Amomum volatile oil 50g, add about 100g sucrose, about 10g dextrin, add and mix evenly according to the same amount, granulate and pack separately to get granules, 5g/bag, about 30 bags.

Embodiment 3: the preparation of amomum volatile oil capsule

1 Extraction method: Select Amomunvillosum Lour from Yunnan, crush it, weigh 200g precisely, put it in a round bottom flask, add 1200ml of distilled water, soak for 1 hour, extract by steam distillation for 10h, and collect the volatile oil.

2 Preparation: Insert the capsule body into the capsule plate, put 50g of the volatile oil prepared in method 1 on the capsule plate, tap the capsule plate lightly to make the medicine powder fall into the capsule shell, until all the capsule shells are filled with the medicine powder , put on the capsule cap, 0.5g/capsule, 100 capsules in total.

Embodiment 4: pharmacological experiment

(1) Effects of amomum on weight loss, lipid reduction and serum endotoxin content reduction in obese rats

90 SD rats were used in the experiment, and were divided into normal group, model group, low, medium and high dose (4,8,16mg/kg) administration groups of Amomum volatile oil (prepared in Example 2) (VOA) extract, Amomum Water extract (prepared in Example 1) (WEA) low, medium and high dose (24,48,96mg/kg) administration group, probiotics golden bifidum (GLB) positive control group (450mg/kg), except the normal group , each group was fed high-fat feed (10% lard, 10% egg yolk, 1% cholesterol, 79% basal feed) until the end of the experiment. Body weight and food intake were recorded daily during the experiment; blood was collected from the inner canthus of all rats on an empty stomach every two weeks, and the amount of blood collected was about 1.5-2ml each time.

The collected blood was centrifuged at 10,000r/min at 4°C for 15min in a high-speed desktop refrigerated centrifuge, and the supernatant was tested for serum by a triglyceride kit (GOP-POD method) and a total cholesterol kit (GOP-POD method). Triglyceride (TG), total cholesterol (TC) content.

After 12 weeks of the experiment, the rats were anesthetized with chloral hydrate solution, and blood from the hepatic portal vein was collected using a disposable vacuum venous blood collection tube, centrifuged at 2000r/min for 10min, and the upper layer of plasma was taken, and the content of intestinal-derived endotoxin (LPS) was detected by Limulus reagent.

As shown in Figure 1, feeding SD rats with high-fat diet and giving different water extracts of Amomum can effectively reduce the body weight of the rats and the contents of TG and TC in the blood. At the same time, the food intake of the Amomum group is close to that of the model group, but The weight gain is not obvious, and the ratio of food intake to body weight is high. Among them, Amomum volatile oil extract has the most significant effect on reducing rat body weight, TG, and TC. After feeding SD rats with high-fat diet for 12 weeks, the LPS content of the model group increased significantly, while the Amomum water extract group inhibited the increase of LPS to a certain extent, among which the volatile oil of Amomum had the most significant inhibitory effect. The results showed that Amomum water extract and Amomum volatile oil (the latter effect is more prominent), can significantly control the obesity induced by high-fat diet, and can relieve the hyperlipidemia in the obese state, and at the same time inhibit the obesity in the obese state. High serum endotoxin state.

(2) In vitro inhibitory activity of amomum on pancreatic lipase

Through in vitro experiments on the inhibition of lipase activity by Amomum volatile oil, water extract and the positive drug orlistat, the release of oleic acid from triglycerides was used as an index to evaluate the effect of Amomum volatile oil extract and water extract on lipase activity. Pancreatic lipase activity.

Prepare 300units/mL pancrelipase solution. Make fat emulsion according to the existing report method, accurately weigh 80 mg triolein, 10 mg lecithin, and 5 mg taurocholic acid, place them in a dry small rotary evaporator, and evaporate to dryness in a water bath at 60 °C. Add 9mL of 0.1mol/L deTris-hydrochloric acid solution (PH7.0), ultrasonic treatment for 5min to make it fully emulsified. Finally, 100uL of fat emulsion, 50uL of pancrelipase solution, and 100uL of water were added to the reaction system, which was used as a blank control; in addition, 100uL of Amomum volatile oil extract, water extract and positive drug of different concentrations were added to the reaction tube to obtain test group. All centrifuge tubes were incubated at a constant temperature of 37°C for 30 minutes. After the reaction, operate according to the instructions, use the free fatty acid kit to measure the released fatty acid content, take 3 samples each time, take the average value to indicate the enzyme activity, and draw the water extract of Amomum japonicus and Austrian Inhibition curve of lixistat on pancreatic lipase activity.

As shown in Figure 6, the abscissa is the concentration of different extracts of Amomum amomum, and the ordinate is the relative activity percentage of pancreatic lipase. It can be seen that both the volatile oil and water extract of Amomum amomum have an inhibitory effect on pancreatic lipase, and there is a dose effect. Among them, Amomum volatile oil has a better inhibitory effect. It can be seen that the volatile oil and water extract of amomum can inhibit the decomposition of fat in food by intestinal lipase, thereby inhibiting the synthesis of TG and TC, thereby reducing body weight.

Except for Figures 1-6, the experimental data of the present invention can refer to the following Tables 1-4.

Table 1 Amomum different extracts on rat body weight (g, n=10)

Continued Table 1

Compared with normal group, ^* p<0.05, ^** p<0.01, ^*** p<0.001

^# p<0.05, ^## p<0.01, ^### p<0.001 compared to the model group

Table 2 Amomum different extracts on the impact of rat food intake body weight ratio (%, n=10)

Continued Table 2

Table 3 Amomum different extracts have effect on rat TG, TC (mmol/L, n=10) and LPS (EU, n=10) The effect of content

Continued Table 3

Compared with normal group, ^* p<0.05, ^** p<0.01, ^*** p<0.001

^# p<0.05, ^## p<0.01, ^### p<0.001 compared to the model group

Table 4 Amomum different extracts in vitro on the influence of pancreatic lipase activity (%, n=10)

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the essential technical content of the present invention. The essential technical content of the present invention is broadly defined in the scope of the claims of the application, and any technical entity completed by others or method, if it is exactly the same as that defined in the scope of the claims of the application, or an equivalent change, it will be deemed to be included in the scope of the claims.

Claims (8)

1. A traditional Chinese medicine composition for weight loss, characterized in that the bulk drug comprises amomum. 2. The application of Amomum water extract or volatile oil in the preparation of weight-loss or blood-lipid-lowering medicine. 3. The application according to claim 2, characterized in that, the preparation method of the Amomum aqueous extract comprises the following steps: accurately weigh 50-150g of Amomum after being pulverized, add 250-1000ml of distilled water to soak for half an hour, After boiling, decoct with low heat for 5-10 minutes, filter the medicinal liquid to obtain filtrate A and medicinal residue A; The distilled water is decocted for 5-10 minutes, and the medicinal liquid is filtered to obtain filtrate C; the filtrates A, B, and C are combined, concentrated, and freeze-dried, which is the water extract of Amomum japonicus. 4. The application according to claim 2, characterized in that the preparation method of the Amomum volatile oil comprises the following steps: accurately weigh 50-150g of the amomum pulverized and place it in a round bottom flask, add 200-800ml of distilled water , Soak for 0.5-1 hour, use steam distillation to extract 5-10h, collect the volatile oil, which is Amomum volatile oil. 5. A Amomum aqueous extract with weight loss and blood fat-lowering effects, characterized in that, the preparation method of the Amomum aqueous extract comprises the following steps: accurately weigh 50-150g of Amomum after crushing, add distilled water Soak 250-1000ml for half an hour, boil and decoct with low heat for 5-10 minutes, filter the medicinal liquid to obtain filtrate A and medicinal residue A; add distilled water to medicinal residue A and decoct for 5-10 minutes, filter the medicinal liquid to obtain filtrate B and medicinal Dregs B; add distilled water to the dregs B and decoct for 5-10 minutes, filter the medicinal liquid to obtain filtrate C; combine filtrates A, B, and C, concentrate, and freeze-dry to obtain the water extract of amomum. 6. A Amomum volatile oil with weight loss and blood fat-lowering effects, characterized in that, the preparation method of the Amomum volatile oil comprises the following steps: accurately weigh 50-150g of Amomum pulverized and place it in a round-bottomed flask, Add 200-800ml of distilled water, soak for 0.5-1 hour, extract by steam distillation for 5-10 hours, collect volatile oil, which is amomum volatile oil. 7. A method for preparing the weight-loss or blood-lipid-lowering medicine described in claim 2, characterized in that, the production method is specifically carried out in accordance with the following steps: after the amomum is pulverized, 50-150g is accurately weighed, and distilled water is added for 250- Soak 1000ml for half an hour, boil and decoct with low heat for 5-10 minutes, filter the medicinal liquid to obtain filtrate A and medicinal residue A; add distilled water to decoct medicinal residue A for 5-10 minutes, filter the medicinal liquid to obtain filtrate B and medicinal residue B Add distilled water to the dregs B and decoct for 5-10 minutes, filter the liquid to obtain filtrate C; combine the filtrates A, B, and C, concentrate, and freeze-dry to obtain the water extract of Amomum sativa. 8. A method for preparing the weight-loss or blood-lipid-lowering medicine described in claim 2, characterized in that, the production method is specifically carried out according to the following steps: after crushing the amomum, 50-150 g is accurately weighed and placed in a round-bottomed flask Add distilled water 200-800ml, soak for 0.5-1 hour, use steam distillation to extract 5-10h, collect volatile oil, which is amomum volatile oil.