CN105166466A

CN105166466A - Feed additive capable of improving meat quality

Info

Publication number: CN105166466A
Application number: CN201510513352.1A
Authority: CN
Inventors: 肖俊峰; 周盛昌; 肖丽萍; 施建成; 杨林; 姜文; 吴有林; 叶俊标
Original assignee: Zhangzhou Aonong Animal Husbandry Technology Co Ltd
Current assignee: Zhangzhou Aonong Animal Husbandry Technology Co Ltd
Priority date: 2015-08-20
Filing date: 2015-08-20
Publication date: 2015-12-23

Abstract

The invention discloses a feed additive capable of improving the meat quality. The feed additive is prepared by mixing the following raw materials in percentage by weight: 5 to 25% of beta-hydroxyl-beta-methyl calcium butyrate (HMB-Ca), 10 to 30% of carvacrol, 30 to 50% of garcinia cambogia extract, and 15 to 35% of carrier. By adding the provided feed additive in common pig feed, the growth speed of pigs in large-scale and intensive farms is accelerated; moreover, the stress-resistant performance of pigs is enhanced, the fat metabolism efficiency is improved, the lean meat content is increased, the organism oxidation is delayed, the protein precipitation is promoted, and the meat quality is improved.

Description

A kind of feed addictive improving meat

Technical field

The present invention relates to the technical field of feed, refer in particular to a kind of feed addictive improving meat.

Background technology

Along with growth in the living standard, the concern of people to breeding production benefit and meat gets more and more, consumer is not only confined to nutrition and the security of meat to the pursuit of pork, and increasing consumer starts to pursue the external sense organ (lean meat percentage) of meat and inherent local flavor.Under intensive cultivating condition, the stress factor (change suddenly as immunity inoculation, stocking density, humiture and frighten) that animal is subject to also gets more and more.Under stressed condition, the redox equilibrium state of livestock and poultry body is damaged, and causes body generation response to oxidative stress, and meat declines, and production performance reduces.

Summary of the invention

The object of this invention is to provide a kind of feed addictive improving meat, to improve growth speed of pigs under scale and intensive condition, strengthen stress resistance of pigs, improve lean meat percentage.

To achieve these goals, solution of the present invention is:

Improve a feed addictive for meat, mixed by following raw material by mass percentage,

Beta-hydroxy-Beta-methyl calcium butyrate (HMB-Ca) 5-25%;

Carvacrol 10-30%;

HCA extract 30-50%;

Carrier 15-35%.

Described carrier is defatted rice bran.

Described HCA method for preparing extractive: get ripe HCA 1 part, remove wherein seed, then smash to pieces in a reservoir, add 3 parts of distilled water, continue to stir into pasty state, last 65 DEG C are dried into powder.

The present invention is applied in the basal diet premixed feed of growing and fattening pigs, and by weight percentage, adding proportion is 1%.

Operation principle of the present invention:

Beta-hydroxy-Beta-methyl calcium butyrate (HMB-Ca) is a kind of intermediate product of leucine metabolism, belongs to butyrates material.Leucine is branched-chain amino acid important in human body, is also one of necessary amino acid of animal.Gallagher etc. (2000) study discovery, and HMB can promote protein to synthesize and reduce it to decompose, and accelerates fat consumption, delays muscular fatigue, keep cell membrane integrity, contribute to improving endurance, have significant body building action.

Carvacrol is a kind of material extracted from wild marjoram, has antibacterial and oxidation resistant effect.Carvacrol has activity at the skin of intestinal epithelial cell, can accelerate the turnover rate of intestinal epithelial cell, reduces pathogen to the infection of intestinal epithelial cell, strengthens body to the digestion power of nutriment.

The extract of HCA---hydroxycitric acid can acute activation adrenaline, promote catabolism of fat, malic acid can promote the activity of mitochondrial fatty catabolic enzyme, in addition, cellulose can make animal produce full sense, thus reduces Energy intaking too much and cause lean meat percentage to decline.

Adopt after such scheme, add product of the present invention in conventional fattening pannage after, growth speed of pigs under scale and intensive condition can be improved; strengthen stress resistance of pigs, improve fat metabolism efficiency, improve lean meat percentage; delay body oxidation, promote proteins deposited, improve meat.

Detailed description of the invention

Test method:

360 kinds are outer ternary " DLY ", and body weight is the healthy growth pig of 80 ± 5kg, are divided into 6 processed group at random, each processed group 6 repetition, each repetition 10 pigs.5 kinds of different premix material additives add basic test daily ration (with reference to U.S. NRC(2012) pig nutritional need respectively to) in, basal diet composition is in table 1, and additive composition is in table 2.50 days experimental periods.Duration of test feeding and management program and immune programme for children carry out with reference to conventional market pig feeding and management handbook, dead head number and feed consumption rate in record experimental period.

During off-test, the pig in all repetitions is weighed, calculate each processed group average daily gain and feed efficiency.Repeat selection 3 pigs butcher from each, measure the thickness of backfat, go longissimus dorsi muscle to measure meat.Moisture, succulence, mouthfeel tenderness, Chewiness and local flavor etc. after evaluation yellowish pink, intramuscular fat, tenderness, extreme value pH, protein solubility, drip loss, Coefficient shrinkage, dry matter content, total fat amount, cooking loss, the cooking.Measure Antioxidant Indexes in serum lipid metabolism index and serum and liver.

In serum, T-CHOL (TC), triglycerides (TG), HDL-C (HDL-C) and LDL-C (LDL-C) all use kit (Beijing BHKT clinical reagent Co., Ltd) to measure, and in automatic clinical chemistry analyzer (Hitachi 7600-020, Hitachi, Ltd, Tokyo, Japan) upper analysis.

Total number born (T-SOD) activity is measured by xanthine/xanthine oxidase system, system produces ultra-oxygen anion free radical, nitroblue tetrazolium can be reduced into formazan, its absorbance (Sun etc., 1988) can be detected with spectrophotometer in 550nm place.

MDA (MDA) content adopts the method for thiobarbituricacidα-to measure absorbance (Placer etc., 1966) at 532nm place.

The process of mensuration based on isopropyl benzene hydroperoxide catalytic oxidation-reduction type glutathione (GSH) that glutathione peroxidase (GSH-Px) is active and the process phase coupling (Lawrence etc. of NADPH reduction-oxidation type glutathione, 1976), GSH-Px unit of activity is expressed as the molal quantity of the NADPH of oxidation per minute.

The Scavenging activity kit (Bioengineering Research Institute is built up in Nanjing) of ultra-oxygen anion free radical measures, assay method carries out in strict accordance with kit description, principle is xanthine and xanthine oxidase reaction system in simulation body, produce ultra-oxygen anion free radical, add electron transport substance and developer, make reaction system present aubergine, measure its absorbance with spectrophotometric.

Hydroxyl radical-scavenging ability adopts kit measurement (Bioengineering Research Institute is built up in Nanjing) equally, and its principle mainly utilizes Fenton to react and produces hydroxy radical, H ₂o ₂amount and the hydroxy radical amount that produces of Fenton reaction be directly proportional, after giving electron acceptor, use griess reagent colour development, form red material, its colour generation and hydroxy radical number proportional.

Adopt SAS9.0(2001) middle ANOVA analytical test data, and carry out Multiple range test by DUNCANShi method, the level of signifiance p=0.05.

Test beta-hydroxy-Beta-methyl calcium butyrate and carvacrol derive from feed market retailer, and non-immediate obtains from manufacturer.

HCA method for preparing extractive: get ripe HCA (1 part), remove wherein seed, then smash to pieces in a reservoir, add 3 parts of distilled water, continue to stir into pasty state, last 65 DEG C are dried into powder.

Table 1 basal diet composition (body weight 80kg ~ deliver for sale) %

Daily ration forms	Control group	Processed group (5 groups)
			Corn	70.6	70.6
Dregs of beans 46.5%	18	18
			Wheat bran	6	4
Stone flour	1	1
			Calcium monohydrogen phosphate	1	1
Salt	0.4	0.4
			Premix	3	3
Additive	0	2(5 kind various combination)
			Add up to	100	100

Wherein, described dregs of beans 46.5% refers to protein content 46.5%.Here premix refers to the common growing and fattening pigs premix of commercial type, mostly all have references to NRC(2012) pig nutritional need and NY/T2004 " swine rearing standard " premix of preparing.

Table 23% premix formulation:

Material name	Unit	Content
			Vitamin A	KIU/kg	600
Vitamin D	KIU/kg	140
			Vitamin E	mg/kg	700
Vitamin K3	mg/kg	140
			Vitamin B1	mg/kg	50
Vitamin B2	mg/kg	150
			Vitamin B6	mg/kg	100
Vitamin B12	mg/kg	0.4
			Nicotinic acid	mg/kg	1000
Pantothenic acid	mg/kg	1500
			Folic acid	mg/kg	25
Biotin	mg/kg	2
			Choline Chloride	g/kg	20
Ferrous glycine	g/kg	8
			Zinc methionine	g/kg	7
Chelating amino acids manganese	g/kg	3
			Copper methionine	g/kg	0.2
Yeast selenium	mg/kg	5
			Potassiumiodate	mg/kg	25
Zeolite powder	g/kg	300
			Corn core carrier	g/kg	580

Table 3 five groups of different additive composition %

Additive forms	1	2	3	4	5
						Beta-hydroxy-Beta-methyl calcium butyrate	5	10	15	20	25
Carvacrol	10	15	20	25	30
						HCA extract	50	45	40	35	30
Defatted rice bran carrier	35	30	25	20	15
						Add up to	100	100	100	100	100

result:

Test item	Control group	1	2	3	4	5	SEM	P value
									Original body mass kg	80.15	81.73	77.90	79.96	82.46	81.74	1.2	0.82
The heavy kg in test end	131.34	130.52	127.18	128.92	134.39	136.13	4.5	0.13
									Average daily gain g	1023.8	975.8	985.6	979.2	1038.6	1087.8	94.3	0.08
Feed efficiency kg/kg	3.35	3.26	3.23	3.20	3.22	3.29	0.11	0.73
									Yellowish pink	3.58	3.82	4.28	4.04	4.33	4.65	0.68	0.07
Marble grain	2.63	2.73	2.85	2.71	2.69	2.70	0.14	0.73
									pH	6.23	6.41	6.55	6.45	6.43	6.30	0.09	0.85
Drip loss	0.90	0.84	0.62	0.73	0.79	0.81	0.11	0.04
									Percentage of water loss %	16.62	15.23	10.39	13.75	13.34	14.43	3.55	0.02
Lean meat percentage %	68.34	69.55	70.34	69.36	70.37	70.75	0.92	0.49
									L	51.01	50.95	50.77	51.35	52.47	51.26	0.56	0.69
a	13.98	14.17	13.96	14.03	13.85	14.52	0.32	0.73
									b	5.52	5.47	5.56	5.41	5.54	5.59	0.08	0.89
TG mmol/L	12.34	8.31	6.34	7.62	7.87	6.94	2.75	≤0.01
									TC mmol/L	2.85	2.37	2.15	2.36	2.40	2.36	0.23	0.03
LDL-C mmol/L	2.45	2.68	3.39	3.41	3.88	3.75	0.76	≤0.01
									HDL-C mol/L	0.17	0.25	0.29	0.22	0.21	0.24	0.04	0.25
T-SOD U/mL	178.19	248.21	263.15	255.33	231.49	229.76	52.7	≤0.01
									GSH-Px U/mL	1530	1830	2112	1989	2236	2337	278.3	≤0.01
Hydroxyl radical-scavenging ability U/mL	498	576	788	642	621	695	87.6	≤0.01
									Superoxide anion resisitance Scavenging activity U/mL	394	432	556	549	603	613	102.7	≤0.01
MDA mmol/L	11.4	7.5	7.1	5.6	5.1	5.8	3.8	≤0.01

In experimental period, mortality of animals (0.2%) is not by the impact of daily ration process.But different disposal additive on piglet growth impact significantly, and the speed of growth wherein processing 3 groups is the fastest, and feed efficiency is higher.From indexs such as yellowish pinks, process 4 performance better; From Biochemical Indices In Serum, after adding feed addictive of the present invention, along with the increase of HMB-Ca addition, in serum and hepatic homogenate liquid T-SOD and GSH-Px actively significantly to increase ( p<0.05), superoxide anion resisitance and suppress hydroxyl radicals significantly strengthen ( p<0.05), lipid peroxidation product MDA content significantly reduce ( p<0.05).This shows, processes 4 additive combinations and is promoting growth of animal, improves lean meat percentage, improving best results in meat.

Claims

1. improve a feed addictive for meat, it is characterized in that: mixed by following raw material by mass percentage,

Beta-hydroxy-Beta-methyl calcium butyrate HMB-Ca5-25%;

Carvacrol 10-30%;

HCA extract 30-50%;

Carrier 15-35%.

2. a kind of feed addictive improving meat as claimed in claim 1, is characterized in that: described carrier is defatted rice bran.

3. a kind of feed addictive improving meat as claimed in claim 1, it is characterized in that: described HCA method for preparing extractive: get ripe HCA 1 part, remove wherein seed, then smash to pieces in a reservoir, add 3 parts of distilled water, continue to stir into pasty state, last 65 DEG C are dried into powder.

4. a kind of feed addictive improving meat as claimed in claim 1, is characterized in that: be applied in the basal diet premixed feed of growing and fattening pigs, by weight percentage, adding proportion is 1%.