CN105158891A - Three-channel microscope interface - Google Patents
Three-channel microscope interface Download PDFInfo
- Publication number
- CN105158891A CN105158891A CN201510239611.6A CN201510239611A CN105158891A CN 105158891 A CN105158891 A CN 105158891A CN 201510239611 A CN201510239611 A CN 201510239611A CN 105158891 A CN105158891 A CN 105158891A
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- CN
- China
- Prior art keywords
- light
- dichroic mirror
- triple channel
- shell
- reflected light
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/362—Mechanical details, e.g. mountings for the camera or image sensor, housings
Abstract
The invention discloses a three-channel microscope interface which includes a housing, a beam splitting module and a tube lens. Three light holes are disposed in the housing. The light splitting module is disposed in the housing, and includes a first dichroscope and a second dichroscope which are arranged in parallel. Incident mixed light passes through the tube lens and falls onto the first dichroscope to produce first transmitting light and first reflecting light. The first transmitting light emits from the light holes at the corresponding position on the housing. The first reflecting light falls onto the second dichroscope to produce second transmitting light and second reflecting light. The second transmitting light and the second reflecting light emit respectively through the light holes at the corresponding positions on the housing. Optical paths of the incident light from the tube lens to image planes of the light holes are the same. The interface allows three cameras to share one imaging tube lens, the imaging amplification factors of the channels are exactly the same, and when large samples are subjected to scan imaging, no problem will occur in the stitching of the channels and the matching of all the channels.
Description
Technical field
The invention belongs to optical imaging field, more specifically, relate to a kind of triple channel microscope interfaces.
Background technology
Microphotography is indispensable part in the use of modern microscopes.Due to the development of fluorescence imaging, now in the multispectral color separation imaging field of the speed of pursuit, the polychrome micro-image of such as large sample obtains or the polychrome micro-image of a large amount of sample obtains, and requires three wave band imagings simultaneously.
But at present, still do not have microscope interfaces to realize triple channel imaging, especially for scientific research level camera, size is comparatively large, is difficult to make it share the cylinder mirror of an imaging.If we use relaying camera lens, not only complex structure, and the shooting area of three cameras and Nonlinear magnify multiplying power are difficult to regulate, and bring very large problem will to the coupling of image, particularly when the imaging of large sample Overlap-scanning, problem is more obvious.
Summary of the invention
For above defect or the Improvement requirement of prior art, the invention provides a kind of triple channel microscope interfaces, its object is to by certain accurate optic-mechanical design, make three scientific research level cameras can share an imaging cylinder mirror, the color separation imaging while of carrying out, and accurately control machine error ensures the consistent of each passage visual field, solve the technical matters of color separation imaging while of triple channel scientific research level camera thus.
For achieving the above object, according to one aspect of the present invention, provide a kind of triple channel microscope interfaces, it is characterized in that, comprise shell, spectral module and cylinder mirror, described shell have three light holes, described spectral module is arranged on enclosure, comprise the first dichroic mirror and the second dichroic mirror that be arranged in parallel, incident mixed light drops on the first dichroic mirror through cylinder mirror, produce the first transmitted light and the first reflected light, wherein the first transmitted light, by the light hole outgoing of relevant position on shell, first reflected light is broadband light, first reflected light drops on the second dichroic mirror, produce the second transmitted light and the second reflected light, second transmitted light and the second reflected light are respectively by the light hole outgoing of relevant position on shell, first transmitted light, second transmitted light and the second reflected light are the different narrow band of light of wave band, incident light is identical to the light path of each light hole image planes from cylinder mirror.
The root-mean-square value that the face type of described first dichroic mirror and the second dichroic mirror rises and falls is less than 150nm, and the disturbance that the wavefront of face type on the second transmitted light and the second reflected light due to dichroic mirror is caused is unlikely to affect image quality.
Preferably, described cylinder mirror is adjustable to the light path of each light hole image planes.
In general, the above technical scheme conceived by the present invention compared with prior art, because three scientific research level cameras share an imaging cylinder mirror, can obtain following beneficial effect:
Three cameras share an imaging cylinder mirror, and the enlargement factor of each passage imaging is completely the same, and when carrying out large sample scanning imagery, the splicing of each passage and the coupling of all passages there will not be problem.
Preferred version, cylinder mirror is adjustable to the light path of each light hole image planes, can be convenient to use the camera of distinct interface standard.
Accompanying drawing explanation
Fig. 1 is triple channel microscope interfaces structural representation provided by the invention;
Fig. 2 is triple channel microscope interfaces outside drawing in embodiment.
In all of the figs, identical Reference numeral is used for representing identical element or structure, wherein: 201 is the first scientific research and camera, 202 is the second scientific research and camera, 203 is the 3rd scientific research and camera, and 210 is spectral module, and 220 is cylinder mirror, 230 is shell, 211 is the first dichroic mirror, and 212 is the second dichroic mirror, and 213 is the first cutoff filter, 214 is the second cutoff filter, and 215 is the 3rd cutoff filter.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.In addition, if below in described each embodiment of the present invention involved technical characteristic do not form conflict each other and just can mutually combine.
Triple channel microscope interfaces provided by the invention, comprises shell 230, spectral module 210 and cylinder mirror 220.
Described shell has three light holes, described shell has identical camera interface outside each light hole.Preferably, described light hole is provided with the optical filter of the corresponding wave band of emergent light.
Described spectral module is arranged on enclosure, as shown in Figure 1, comprise the first dichroic mirror 211 and the second dichroic mirror 212 be arranged in parallel, incident mixed light drops on the first dichroic mirror 211 through cylinder mirror, produce the first transmitted light and the first reflected light, wherein the first transmitted light, by the light hole outgoing of relevant position on shell 230, first reflected light is broadband light, first reflected light drops on the second dichroic mirror 212, produce the second transmitted light and the second reflected light, second transmitted light and the second reflected light are respectively by the light hole outgoing of relevant position on shell 230, first transmitted light, second transmitted light and the second reflected light are the different narrow band of light of wave band.Incident light is identical to the light path of each light hole image planes from the first dichroic mirror 211.
Preferably, the corresponding light hole of described first transmitted light, the second transmitted light and the second reflected light outgoing is in three Different Plane, is convenient to the scientific research level camera that installation volume is larger; More preferably, described three Different Plane are mutually vertical, make not interfere with each other between camera, are convenient to install and operation.
Described cylinder mirror 220 is adjustable to the light path of each light hole image planes.
Be below embodiment:
Triple channel microscope interfaces, as shown in Figure 1, stereographic map as shown in Figure 2, comprises shell 230, spectral module 210 and cylinder mirror 220 to structure; Described shell 230 have three light holes; It is inner that described spectral module is arranged on shell 230, comprise the first dichroic mirror 211 (high pass be arranged in parallel, the light of more than 593nm wavelength passes through) and image-forming module second dichroic mirror 212 (high pass, the light of more than 495nm wavelength passes through), incident mixing fluorescence drops on image-forming module first dichroic mirror 211 through cylinder mirror 220, produce the first transmitted light and the first reflected light, wherein the first transmitted light, by the light hole outgoing of relevant position on shell 230, imaging surface is imageplane1.Imaging surface imageplane1 places in front the first cutoff filter 213 (band is logical, and the centre wavelength passed through is 641nm, bandwidth 75nm).First reflected light is broadband light, first reflected light drops on image-forming module second dichroic mirror 212, produce the second transmitted light and the second reflected light, second transmitted light and the second reflected light are respectively by the light hole outgoing of relevant position on interface shells 230, and the first transmitted light, the second transmitted light and the second reflected light are the different fluorescence of wave band.Second reflected light is imaged on imageplane2.Imaging surface imageplane2 places in front the second cutoff filter 214 (band is logical, and the centre wavelength passed through is 525nm, bandwidth 50nm).Second transmitted light is imaged on imageplane3.The 3rd cutoff filter 215 (band is logical, and the centre wavelength passed through is 447nm, bandwidth 60nm) is placed in imaging surface imageplane3 front.
Incident light is identical to the light path of each light hole image planes from cylinder mirror 220; Described three scientific research level cameras 201,202,203 are connected by interface with three light holes.
Cylinder mirror 220 be arranged on shell 230 by screw thread, can adjustment barrel mirror identical to the light path of each light hole image planes.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. a triple channel microscope interfaces, is characterized in that, comprises shell (230), spectral module (210) and cylinder mirror (220), described shell have three light holes, described spectral module (210) is arranged on enclosure, comprise the first dichroic mirror (211) and the second dichroic mirror (212) that be arranged in parallel, incident mixed light drops on the first dichroic mirror (211) through cylinder mirror, produce the first transmitted light and the first reflected light, wherein the first transmitted light, by the light hole outgoing of relevant position on shell, first reflected light is broadband light, first reflected light drops on the second dichroic mirror (212), produce the second transmitted light and the second reflected light, second transmitted light and the second reflected light go up the light hole outgoing of relevant position respectively by shell (230), first transmitted light, second transmitted light and the second reflected light are the different narrow band of light of wave band, incident light is identical to the light path of each light hole image planes from cylinder mirror (220).
2. triple channel microscope interfaces as claimed in claim 1, it is characterized in that, described cylinder mirror (220) is adjustable to the light path of the first dichroic mirror (211).
3. triple channel microscope interfaces as claimed in claim 1, is characterized in that, the corresponding light hole of described first transmitted light, the second transmitted light and the second reflected light outgoing is in three Different Plane.
4. triple channel microscope interfaces as claimed in claim 3, it is characterized in that, described three Different Plane are mutually vertical.
5. triple channel microscope interfaces as claimed in claim 1, it is characterized in that, described light hole is provided with the optical filter (213,214,215) of the corresponding wave band of emergent light.
6. triple channel microscope interfaces as claimed in claim 1, it is characterized in that, described shell has identical camera interface outside each light hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510239611.6A CN105158891B (en) | 2015-05-11 | 2015-05-11 | A kind of triple channel microscope interfaces |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510239611.6A CN105158891B (en) | 2015-05-11 | 2015-05-11 | A kind of triple channel microscope interfaces |
Publications (2)
| Publication Number | Publication Date |
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| CN105158891A true CN105158891A (en) | 2015-12-16 |
| CN105158891B CN105158891B (en) | 2017-12-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510239611.6A Expired - Fee Related CN105158891B (en) | 2015-05-11 | 2015-05-11 | A kind of triple channel microscope interfaces |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106873143A (en) * | 2017-04-27 | 2017-06-20 | 广西奥顺仪器有限公司 | A kind of built-in camera and light splitting interface structure |
| CN112923848A (en) * | 2021-01-25 | 2021-06-08 | 上海兰宝传感科技股份有限公司 | Correlation type laser size measurement sensor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101561403A (en) * | 2009-06-01 | 2009-10-21 | 燕山大学 | Three-dimensional orthogonality microscope image pick-up observation device and quantitative image method thereof |
| US20100157422A1 (en) * | 2007-09-05 | 2010-06-24 | Nikon Corporation | Microscope device |
| JP2010169968A (en) * | 2009-01-23 | 2010-08-05 | Olympus Corp | Microscope system and control method thereof |
| CN101940463A (en) * | 2010-10-09 | 2011-01-12 | 华中科技大学 | Fluorescence endoscopic imaging system for living body |
| CN103698307A (en) * | 2013-12-06 | 2014-04-02 | 中国科学院苏州生物医学工程技术研究所 | Laser scanning confocal microscope system |
-
2015
- 2015-05-11 CN CN201510239611.6A patent/CN105158891B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100157422A1 (en) * | 2007-09-05 | 2010-06-24 | Nikon Corporation | Microscope device |
| JP2010169968A (en) * | 2009-01-23 | 2010-08-05 | Olympus Corp | Microscope system and control method thereof |
| CN101561403A (en) * | 2009-06-01 | 2009-10-21 | 燕山大学 | Three-dimensional orthogonality microscope image pick-up observation device and quantitative image method thereof |
| CN101940463A (en) * | 2010-10-09 | 2011-01-12 | 华中科技大学 | Fluorescence endoscopic imaging system for living body |
| CN103698307A (en) * | 2013-12-06 | 2014-04-02 | 中国科学院苏州生物医学工程技术研究所 | Laser scanning confocal microscope system |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106873143A (en) * | 2017-04-27 | 2017-06-20 | 广西奥顺仪器有限公司 | A kind of built-in camera and light splitting interface structure |
| CN112923848A (en) * | 2021-01-25 | 2021-06-08 | 上海兰宝传感科技股份有限公司 | Correlation type laser size measurement sensor |
| CN112923848B (en) * | 2021-01-25 | 2022-05-24 | 上海兰宝传感科技股份有限公司 | Correlation type laser size measurement sensor |
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| CN105158891B (en) | 2017-12-08 |
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Granted publication date: 20171208 |