CN105137063A - Making method of unmarked electrochemical immunosensor for clenbuterol hydrochloride detection - Google Patents
Making method of unmarked electrochemical immunosensor for clenbuterol hydrochloride detection Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
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Abstract
The invention discloses a making method of an unmarked electrochemical immunosensor constructed on the basis of a molybdenum disulfide/silver palladium alloy nanocomposite material, and belongs to the technical fields of novel nanometer function materials and food safety analysis. The sensor made in the invention can be used to detect clenbuterol hydrochloride in a practical sample. The molybdenum disulfide/silver palladium alloy nanocomposite material is prepared through a one-pot technology, and then the simple, fast and sensitive unmarked electrochemical immunosensor for detecting clenbuterol hydrochloride in a meat product is made by using the excellent adsorption and electrochemical catalysis performances of the nanocomposite material.
Description
Technical field
The present invention relates to a kind of preparation method of the unmarked electrochemical immunosensor based on molybdenum disulfide/silver palladium alloy nano composite material structure, prepared sensor can be used for the detection of clenbuterol hydrochloride.Belong to Nano-function thin films and Food Safety Analysis technical field.
Background technology
Clenbuterol hydrochloride is the medicine that a class is called beta-stimulants (β-agonist), instead of a certain specific medicine.Be 16 kinds according to " clenbuterol hydrochloride " kind catalogue that office of the food security council of State Council " " clenbuterol hydrochloride " focus efforts on special areas scheme " (food peace do (2011) No. 14) specifies, comprise: Ractopamine, Clenbuterol, salbutamol, salbutamol sulfate, Dopamine hydrochloride, Cimaterol, bricalin, phenolethanolamine A, bambuterol, hydrochloric acid Zilpaterol, clorprenaline hydrochloride, Mabuterol, western Boot sieve, bromine Boot sieve, tartrate Afromoterol, formoterol fumarate.Because they can promote the proteins deposited and lipolysis of animal body, suppress fat deposition, the lean meat percentage of trunk can be significantly improved, increase weight and improve food conversion ratio, be used as growth accelerator, the feed addictive of the livestock and poultry such as ox, sheep, pig.But clenbuterol hydrochloride has the spinoff of danger close, when people eat the meat products containing clenbuterol hydrochloride, the damage of cardiovascular system can be caused, and serious nervous symptoms may be occurred.Therefore, research and development detection method that is quick, Sensitive Detection clenbuterol hydrochloride is most important for daily life.
Electrochemical immunosensor is widely used in the field such as food analysis and environment measuring due to advantages such as it are highly sensitive, specificity is good, easy and simple to handle, wherein, unmarked electrochemical immunosensor, because of its preparation advantage such as little relatively simple, affected by environment, has more wide application prospect.Build unmarked electrochemical immunosensor, crucial technology be improve modified electrode antagonist fixed amount and to the signal response speed of test end liquid and size.
Molybdenum disulfide, chemical formula is MoS
2, English name is molybdenumdisulfide, is the principal ingredient of molybdenite, is one of most widely used kollag.Its nanometer two-dimensional structure is the semiconductor nano material of excellent performance, except having large specific surface area, as the carrier of catalyzer and biological antibody, can improve charge capacity, also having excellent electron transmission performance simultaneously as promotor.
At present, most synthesizing mean is all after separately synthesis, then catalyzer and carrier are carried out compound, and process is loaded down with trivial details, and productive rate is not high.Therefore, prepared by the catalyzer with superior catalytic performance for one kettle way to be with a wide range of applications and important scientific meaning.
Summary of the invention
The object of the present invention is to provide a kind of preparation method that can be used for the unmarked electrochemical immunosensor detecting clenbuterol hydrochloride preparing simple, highly sensitive, high specificity.Based on this object, the present invention first adopts one kettle way to prepare molybdenum disulfide/silver palladium alloy nano composite material MoS
2/ AgPd, and utilize it to modify electrode, effectively improve the specific surface area of electrode, increase the adsorbance of antibody; And greatly enhance the electronic response speed of electrode and the performance such as electro-catalysis signal intensity to test end liquid; Then by suction-operated, fixing clenbuterol hydrochloride antibody.When detecting, utilizing antibody to be combined with the specific quantification of antigen, making electrode pair H
2o
2the corresponding reduction of current responsing signal, thus achieve the structure adopting unmarked electrochemical method to detect the immunosensor of clenbuterol hydrochloride.
The technical solution used in the present invention is as follows:
1., for a preparation method for the unmarked electrochemical immunosensor of clenbuterol hydrochloride detection, it is characterized in that, preparation process is:
(1) get the cetyl trimethyl ammonium bromide CTAB solution of 35mL, add 0.01 ~ 0.03g silver nitrate AgNO
3with the chlorine palladium acid H of 2 ~ 6mL
2pdCl
4solution, stirred after 15 minutes, continued to stir the ascorbic acid solution also in succession adding 2 ~ 6mL, the sodium molybdate Na of 0.5 ~ 1.5mL
2moO
4solution and 0.01 ~ 0.03g sodium sulphide Na
2s, stirs after 15 minutes, puts into reactor, at 150 ~ 220 DEG C, reacts 12 ~ 16 hours; After being cooled to room temperature, using deionized water centrifuge washing, at 40 DEG C, carry out vacuum drying, be i.e. obtained molybdenum disulfide/silver palladium alloy nano composite material MoS
2/ AgPd, is dissolved in deionized water and is obtained MoS
2/ AgPd solution;
(2) with the glass-carbon electrode of diameter 4mm for working electrode, drip the MoS of painting 5 ~ 12 μ L at electrode surface
2/ AgPd solution, dries under room temperature;
(3) the electrode washed with de-ionized water will obtained in step (2), dries film forming under room temperature, drips the clenbuterol hydrochloride antibody-solutions of painting 5 ~ 12 μ L at electrode surface, preserves and dry in 4 DEG C of refrigerators;
(4) the electrode washed with de-ionized water will obtained in step (3), continue the bovine serum albumin(BSA) BSA solution dripping painting 5 ~ 10 μ L at electrode surface, preserve in 4 DEG C of refrigerators and dry, by washed with de-ionized water, film forming is dried, obtained unmarked electrochemical immunosensor in 4 DEG C of refrigerators;
The concentration of described CTAB solution is 0.1mol/L, described H
2pdCl
4concentration is 0.03mol/L, and described ascorbic acid solution concentration is 0.3mol/L, described Na
2moO
4solution concentration is 0.1mol/L;
Described MoS
2/ AgPd solution concentration is 1mg/mL;
Described clenbuterol hydrochloride antibody-solutions concentration is 10 μ g/mL;
Described BSA solution concentration is 100 μ g/mL.
2. the clenbuterol hydrochloride electrochemical immunosensor prepared by preparation method of the present invention, is characterized in that, the purposes of described unmarked electrochemical immunosensor is quick, the Sensitive Detection that can be used for clenbuterol hydrochloride.
3. the purposes of unmarked electrochemical immunosensor of the present invention, is characterized in that, the detecting step detecting clenbuterol hydrochloride is:
(1) standard solution preparation: prepare the clenbuterol hydrochloride standard solution that a group comprises the variable concentrations of blank standard specimen, end liquid is the phosphate buffered solution of pH7.4;
(2) working electrode is modified: using unmarked electrochemical immunosensor of the present invention as working electrode, is dripped respectively by the clenbuterol hydrochloride standard solution of the variable concentrations of preparation in step (1) and is coated onto working electrode surface, preserve in 4 DEG C of refrigerators;
(3) working curve is drawn: using saturated calomel electrode as contrast electrode, platinum electrode is as auxiliary electrode, with step (2) the working electrode modified form three-electrode system, connect electrochemical workstation, the PBS buffer solution and the 20 μ L concentration that successively add 10 ~ 25mLpH=7.0 ~ 8.0 are in a cell the H of 3 ~ 6mol/L
2o
2; The working electrode of assembling is detected to H by chronoamperometry
2o
2response; The response current of blank standard specimen is designated as
i 0, the response current of the clenbuterol hydrochloride standard solution containing variable concentrations is denoted as
i i, the difference that response current reduces is Δ
i=
i 0-
i i, Δ
iwith the mass concentration of clenbuterol hydrochloride standard solution
cbetween linear, draw Δ
i-
cworking curve;
(4) detection of clenbuterol hydrochloride: replace the clenbuterol hydrochloride standard solution in step (1) with testing sample, detects according to the method in step (2) and (3), according to the difference DELTA that response current reduces
iand working curve, obtain the content of clenbuterol hydrochloride in testing sample.
4. the purposes of unmarked electrochemical immunosensor of the present invention, it is characterized in that, described clenbuterol hydrochloride is selected from one of following: Ractopamine, Clenbuterol, salbutamol, salbutamol sulfate, Dopamine hydrochloride, Cimaterol, bricalin, phenolethanolamine A, bambuterol, hydrochloric acid Zilpaterol, clorprenaline hydrochloride, Mabuterol, western Boot sieve, bromine Boot sieve, tartrate Afromoterol, formoterol fumarate
useful achievement of the present invention
(1) preparation method of unmarked electrochemical immunosensor of the present invention, preparation process is simple, easy to operate, and prepared sensor can realize detecting quick, sensitive, the high selectivity of clenbuterol hydrochloride in sample, has market development prospect.
(2) the present invention adopts one kettle way to prepare MoS first
2/ AgPd, and be applied to, in the preparation of unmarked electrochemical immunosensor, utilize MoS
2the bigger serface of/AgPd, to increase the adsorbance of antibody, high catalytic activity to improve electrochemical response signal intensity and high electron transport rate thereof to improve electrochemical response signal speed, significantly enhances electrode pair H
2o
2response speed and intensity, substantially increase the detection sensitivity of electrochemical sensor, there is important scientific meaning and using value.
Embodiment
embodiment 1moS
2the preparation method of/AgPd
Get the CTAB solution that 35mL concentration is 0.1mol/L, add the AgNO of 0.01g
3be the H of 0.03mol/L with 2mL concentration
2pdCl
4solution, stirred after 15 minutes, continued stir and in succession add the ascorbic acid solution that 2mL concentration is 0.3mol/L, and 0.5mL concentration is the Na of 0.1mol/L
2moO
4the Na of solution and 0.01g
2s, stirs after 15 minutes, puts into reactor, at 180 DEG C, reacts 14 hours; After being cooled to room temperature, using deionized water centrifuge washing, at 40 DEG C, carry out vacuum drying, be i.e. obtained MoS
2/ AgPd, is dissolved in deionized water and is obtained the MoS that concentration is 1mg/mL
2/ AgPd solution.
embodiment 2moS
2the preparation method of/AgPd
Get the CTAB solution that 35mL concentration is 0.1mol/L, add the AgNO of 0.02g
3be the H of 0.03mol/L with 4mL concentration
2pdCl
4solution, stirred after 15 minutes, continued stir and in succession add the ascorbic acid solution that 4mL concentration is 0.3mol/L, and 1.0mL concentration is the Na of 0.1mol/L
2moO
4the Na of solution and 0.02g
2s, stirs after 15 minutes, puts into reactor, at 150 DEG C, reacts 16 hours; After being cooled to room temperature, using deionized water centrifuge washing, at 40 DEG C, carry out vacuum drying, be i.e. obtained MoS
2/ AgPd, is dissolved in deionized water and is obtained the MoS that concentration is 1mg/mL
2/ AgPd solution.
embodiment 3moS
2the preparation method of/AgPd
Get the CTAB solution that 35mL concentration is 0.1mol/L, add the AgNO of 0.03g
3be the H of 0.03mol/L with 6mL concentration
2pdCl
4solution, stirred after 15 minutes, continued stir and in succession add the ascorbic acid solution that 6mL concentration is 0.3mol/L, and 1.5mL concentration is the Na of 0.1mol/L
2moO
4the Na of solution and 0.03g
2s, stirs after 15 minutes, puts into reactor, at 220 DEG C, reacts 12 hours; After being cooled to room temperature, using deionized water centrifuge washing, at 40 DEG C, carry out vacuum drying, be i.e. obtained MoS
2/ AgPd, is dissolved in deionized water and is obtained the MoS that concentration is 1mg/mL
2/ AgPd solution.
embodiment 4the preparation method of unmarked electrochemical immunosensor
(1) with the glass-carbon electrode of diameter 4mm for working electrode, to drip in painting 5 μ L embodiment 1 obtained MoS at electrode surface
2/ AgPd solution, dries under room temperature;
(2) the electrode washed with de-ionized water will obtained in step (1), dries film forming under room temperature, drips the clenbuterol hydrochloride antibody-solutions that painting 5 μ L concentration is 10 μ g/mL, preserve and dry in 4 DEG C of refrigerators at electrode surface;
(3) the electrode washed with de-ionized water will obtained in step (2), continue to drip at electrode surface the BSA solution that painting 5 μ L concentration is 100 μ g/mL, preserve in 4 DEG C of refrigerators and dry, by washed with de-ionized water, film forming is dried, obtained unmarked electrochemical immunosensor in 4 DEG C of refrigerators.
embodiment 5the preparation method of unmarked electrochemical immunosensor
(1) with the glass-carbon electrode of diameter 4mm for working electrode, to drip in painting 8 μ L embodiment 2 obtained MoS at electrode surface
2/ AgPd solution, dries under room temperature;
(2) the electrode washed with de-ionized water will obtained in step (1), dries film forming under room temperature, drips the clenbuterol hydrochloride antibody-solutions that painting 8 μ L concentration is 10 μ g/mL, preserve and dry in 4 DEG C of refrigerators at electrode surface;
(3) the electrode washed with de-ionized water will obtained in step (2), continue to drip at electrode surface the BSA solution that painting 7 μ L concentration is 100 μ g/mL, preserve in 4 DEG C of refrigerators and dry, by washed with de-ionized water, film forming is dried, obtained unmarked electrochemical immunosensor in 4 DEG C of refrigerators.
embodiment 6the preparation method of unmarked electrochemical immunosensor
(1) with the glass-carbon electrode of diameter 4mm for working electrode, to drip in painting 12 μ L embodiment 3 obtained MoS at electrode surface
2/ AgPd solution, dries under room temperature;
(2) the electrode washed with de-ionized water will obtained in step (1), dries film forming under room temperature, drips the clenbuterol hydrochloride antibody-solutions that painting 12 μ L concentration is 10 μ g/mL, preserve and dry in 4 DEG C of refrigerators at electrode surface;
(3) the electrode washed with de-ionized water will obtained in step (2), continue to drip at electrode surface the BSA solution that painting 10 μ L concentration is 100 μ g/mL, preserve in 4 DEG C of refrigerators and dry, by washed with de-ionized water, film forming is dried, obtained unmarked electrochemical immunosensor in 4 DEG C of refrigerators.
embodiment 7unmarked electrochemical immunosensor prepared by embodiment 4 is used for the detection of Ractopamine, and step is as follows:
(1) standard solution preparation: prepare the Ractopamine standard solution that a group comprises the variable concentrations of blank standard specimen, end liquid is the phosphate buffered solution of pH7.4;
(2) working electrode is modified: using the unmarked electrochemical immunosensor prepared by embodiment 4 as working electrode, is dripped respectively by the Ractopamine standard solution of the variable concentrations of preparation in step (1) and is coated onto working electrode surface, preserve in 4 DEG C of refrigerators;
(3) working curve is drawn: using saturated calomel electrode as contrast electrode, platinum electrode is as auxiliary electrode, with step (2) the working electrode modified form three-electrode system, connect electrochemical workstation, the PBS buffer solution and the 20 μ L concentration that successively add 10mLpH=7.0 are in a cell the H of 3mol/L
2o
2; The working electrode of assembling is detected to H by chronoamperometry
2o
2response; The response current of blank standard specimen is designated as
i 0, the response current of the Ractopamine standard solution containing variable concentrations is denoted as
i i, the difference that response current reduces is Δ
i=
i 0-
i i, Δ
iwith the mass concentration of Ractopamine standard solution
cbetween linear, draw Δ
i-
cworking curve;
(4) detection of Ractopamine: replace the Ractopamine standard solution in step (1) with testing sample, detects according to the method in step (2) and (3), according to the difference DELTA that response current reduces
iand working curve, obtain the content of Ractopamine in testing sample;
Described unmarked electrochemical immunosensor is used for the detection of Ractopamine, and its linear detection range is: 0.06 ~ 100ng/mL, detects and is limited to: 0.02ng/mL.
embodiment 8unmarked electrochemical immunosensor prepared by embodiment 5 is used for the detection of Clenbuterol, and step is as follows:
(1) standard solution preparation: prepare the Clenbuterol standard solution that a group comprises the variable concentrations of blank standard specimen, end liquid is the phosphate buffered solution of pH7.4;
(2) working electrode is modified: using the unmarked electrochemical immunosensor prepared by embodiment 5 as working electrode, is dripped respectively by the Clenbuterol standard solution of the variable concentrations of preparation in step (1) and is coated onto working electrode surface, preserve in 4 DEG C of refrigerators;
(3) working curve is drawn: using saturated calomel electrode as contrast electrode, platinum electrode is as auxiliary electrode, with step (2) the working electrode modified form three-electrode system, connect electrochemical workstation, the PBS buffer solution and the 20 μ L concentration that successively add 15mLpH=7.4 are in a cell the H of 4mol/L
2o
2; The working electrode of assembling is detected to H by chronoamperometry
2o
2response; The response current of blank standard specimen is designated as
i 0, the response current of the Clenbuterol standard solution containing variable concentrations is denoted as
i i, the difference that response current reduces is Δ
i=
i 0-
i i, Δ
iwith the mass concentration of Clenbuterol standard solution
cbetween linear, draw Δ
i-
cworking curve;
(4) detection of Clenbuterol: replace the Clenbuterol standard solution in step (1) with testing sample, detects according to the method in step (2) and (3), according to the difference DELTA that response current reduces
iand working curve, obtain the content of Clenbuterol in testing sample;
Described unmarked electrochemical immunosensor is used for the detection of Clenbuterol, and its linear detection range is: 0.06 ~ 95ng/mL, detects and is limited to: 0.02ng/mL.
embodiment 9unmarked electrochemical immunosensor prepared by embodiment 6 is used for the detection of salbutamol, and step is as follows:
(1) standard solution preparation: prepare the salbutamol standard solution that a group comprises the variable concentrations of blank standard specimen, end liquid is the phosphate buffered solution of pH7.4;
(2) working electrode is modified: using the unmarked electrochemical immunosensor prepared by embodiment 6 as working electrode, is dripped respectively by the salbutamol standard solution of the variable concentrations of preparation in step (1) and is coated onto working electrode surface, preserve in 4 DEG C of refrigerators;
(3) working curve is drawn: using saturated calomel electrode as contrast electrode, platinum electrode is as auxiliary electrode, with step (2) the working electrode modified form three-electrode system, connect electrochemical workstation, the PBS buffer solution and the 20 μ L concentration that successively add 25mLpH=8.0 are in a cell the H of 6mol/L
2o
2; The working electrode of assembling is detected to H by chronoamperometry
2o
2response; The response current of blank standard specimen is designated as
i 0, the response current of the salbutamol standard solution containing variable concentrations is denoted as
i i, the difference that response current reduces is Δ
i=
i 0-
i i, Δ
iwith the mass concentration of salbutamol standard solution
cbetween linear, draw Δ
i-
cworking curve;
(4) detection of salbutamol: replace the salbutamol standard solution in step (1) with testing sample, detects according to the method in step (2) and (3), according to the difference DELTA that response current reduces
iand working curve, obtain the content of salbutamol in testing sample;
Described unmarked electrochemical immunosensor is used for the detection of salbutamol, and its linear detection range is: 0.06 ~ 102ng/mL, detects and is limited to: 0.02ng/mL.
embodiment 10the detection of clenbuterol hydrochloride in pork sample
Accurately take pork sample, adopt conventional method to carry out sample preparation, not add the same sample of clenbuterol hydrochloride for blank, carry out recovery testu, detect according to the step described in embodiment 7 ~ 9, the recovery of clenbuterol hydrochloride in working sample, testing result is in table 1;
The testing result of clenbuterol hydrochloride in table 1 pork sample
Table 1 testing result is known, and the relative standard deviation (RSD) of result is less than 3.4%, and average recovery rate is 98.0 ~ 102%, shows that the present invention can be used for the detection of clenbuterol hydrochloride in pork sample, and highly sensitive, the high specificity of method, result accurately and reliably.
embodiment 11the detection of clenbuterol hydrochloride in beef sample
Accurately take beef sample, adopt conventional method to carry out sample preparation, not add the same sample of clenbuterol hydrochloride for blank, carry out recovery testu, detect according to the step described in embodiment 7 ~ 9, the recovery of clenbuterol hydrochloride in working sample, testing result is in table 2;
The testing result of clenbuterol hydrochloride in table 2 beef sample
Table 2 testing result is known, and the relative standard deviation (RSD) of result is less than 3.2%, and average recovery rate is 98.8 ~ 102%, shows that the present invention can be used for the detection of clenbuterol hydrochloride in beef sample, and highly sensitive, the high specificity of method, result accurately and reliably.
embodiment 12the detection of clenbuterol hydrochloride in meat samples
Accurately take meat samples, adopt conventional method to carry out sample preparation, not add the same sample of clenbuterol hydrochloride for blank, carry out recovery testu, detect according to the step described in embodiment 7 ~ 9, the recovery of clenbuterol hydrochloride in working sample, testing result is in table 3;
The testing result of clenbuterol hydrochloride in table 3 meat samples
Table 3 testing result is known, and the relative standard deviation (RSD) of result is less than 3.3%, and average recovery rate is 97.2 ~ 103%, shows that the present invention can be used for the detection of clenbuterol hydrochloride in meat samples, and highly sensitive, the high specificity of method, result accurately and reliably.
Claims (3)
1., for a preparation method for the unmarked electrochemical immunosensor of clenbuterol hydrochloride detection, it is characterized in that, preparation process is:
(1) get the cetyl trimethyl ammonium bromide CTAB solution of 35mL, add 0.01 ~ 0.03g silver nitrate AgNO
3with the chlorine palladium acid H of 2 ~ 6mL
2pdCl
4solution, stirred after 15 minutes, continued to stir the ascorbic acid solution also in succession adding 2 ~ 6mL, the sodium molybdate Na of 0.5 ~ 1.5mL
2moO
4solution and 0.01 ~ 0.03g sodium sulphide Na
2s, stirs after 15 minutes, puts into reactor, at 150 ~ 220 DEG C, reacts 12 ~ 16 hours; After being cooled to room temperature, using deionized water centrifuge washing, at 40 DEG C, carry out vacuum drying, be i.e. obtained molybdenum disulfide/silver palladium alloy nano composite material MoS
2/ AgPd, is dissolved in deionized water and is obtained MoS
2/ AgPd solution;
(2) with the glass-carbon electrode of diameter 4mm for working electrode, drip the MoS of painting 5 ~ 12 μ L at electrode surface
2/ AgPd solution, dries under room temperature;
(3) the electrode washed with de-ionized water will obtained in step (2), dries film forming under room temperature, drips the clenbuterol hydrochloride antibody-solutions of painting 5 ~ 12 μ L at electrode surface, preserves and dry in 4 DEG C of refrigerators;
(4) the electrode washed with de-ionized water will obtained in step (3), continue the bovine serum albumin(BSA) BSA solution dripping painting 5 ~ 10 μ L at electrode surface, preserve in 4 DEG C of refrigerators and dry, by washed with de-ionized water, film forming is dried, obtained unmarked electrochemical immunosensor in 4 DEG C of refrigerators;
The concentration of described CTAB solution is 0.1mol/L, described H
2pdCl
4concentration is 0.03mol/L, and described ascorbic acid solution concentration is 0.3mol/L, described Na
2moO
4solution concentration is 0.1mol/L;
Described MoS
2/ AgPd solution concentration is 1mg/mL;
Described clenbuterol hydrochloride antibody-solutions concentration is 10 μ g/mL;
Described BSA solution concentration is 100 μ g/mL.
2. a kind of preparation method of unmarked electrochemical immunosensor detected for clenbuterol hydrochloride as claimed in claim 1, it is characterized in that, the purposes of described unmarked electrochemical immunosensor is quick, the Sensitive Detection that can be used for clenbuterol hydrochloride.
3. the purposes of unmarked electrochemical immunosensor as claimed in claim 2, it is characterized in that, described clenbuterol hydrochloride is selected from one of following: Ractopamine, Clenbuterol, salbutamol, salbutamol sulfate, Dopamine hydrochloride, Cimaterol, bricalin, phenolethanolamine A, bambuterol, hydrochloric acid Zilpaterol, clorprenaline hydrochloride, Mabuterol, western Boot sieve, bromine Boot sieve, tartrate Afromoterol, formoterol fumarate.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105353121A (en) * | 2015-12-27 | 2016-02-24 | 济南大学 | Preparation method of biosensor established on basis of silver-amino graphene-molybdenum disulfide and application |
| CN105738351A (en) * | 2016-02-25 | 2016-07-06 | 济南大学 | Preparing method and application of electrochemiluminescence Clenbuterol sensor based on magnetic two-dimensional nanocomposite |
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| CN109254060A (en) * | 2018-11-05 | 2019-01-22 | 济南大学 | A kind of preparation method and application of clenbuterol hydrochloride electrochemical sensing electrode |
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| CN105353121A (en) * | 2015-12-27 | 2016-02-24 | 济南大学 | Preparation method of biosensor established on basis of silver-amino graphene-molybdenum disulfide and application |
| CN105738351A (en) * | 2016-02-25 | 2016-07-06 | 济南大学 | Preparing method and application of electrochemiluminescence Clenbuterol sensor based on magnetic two-dimensional nanocomposite |
| CN105738351B (en) * | 2016-02-25 | 2018-05-25 | 济南大学 | A kind of preparation method and application of the electrogenerated chemiluminescence Clenbuterol sensor based on magnetic two-dimensional nano composite material |
| CN105784993A (en) * | 2016-03-16 | 2016-07-20 | 济南大学 | Production method and application of ractopamine electrochemistry biology immunosensor |
| CN107132260A (en) * | 2017-05-16 | 2017-09-05 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of electrochemical sensor that Ractopamine is detected based on nano material |
| CN107132260B (en) * | 2017-05-16 | 2019-06-18 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of electrochemical sensor based on nano material detection Ractopamine |
| CN108535469A (en) * | 2018-06-29 | 2018-09-14 | 武汉中科志康生物科技有限公司 | A kind of clenbuterol hydrochloride detection device |
| CN109254060A (en) * | 2018-11-05 | 2019-01-22 | 济南大学 | A kind of preparation method and application of clenbuterol hydrochloride electrochemical sensing electrode |
| CN109738641A (en) * | 2019-01-19 | 2019-05-10 | 山东理工大学 | A kind of preparation method and application without enzyme electrochemical immunosensor based on platinum palladium functionalization molybdenum disulfide |
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