CN105136932A - Determination method of natural vitamin E content in sunflower oil - Google Patents
Determination method of natural vitamin E content in sunflower oil Download PDFInfo
- Publication number
- CN105136932A CN105136932A CN201510565653.9A CN201510565653A CN105136932A CN 105136932 A CN105136932 A CN 105136932A CN 201510565653 A CN201510565653 A CN 201510565653A CN 105136932 A CN105136932 A CN 105136932A
- Authority
- CN
- China
- Prior art keywords
- vitamin
- sunflower oil
- sample
- content
- reference substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a field of the natural product content determination, and concretely relates to a determination method of natural vitamin E content in sunflower oil. An extraction condition of the natural vitamin E is optimized by using an ultrasonic method, the content of the natural vitamin E in the sunflower oil sample is determined by using a HPLC method, thereby providing reference for plant oil quality standards. The invention has the advantages of simple step, economic utility and easy popularization.
Description
Technical field
The present invention relates to natural products assay field, be specially a kind of assay method detecting natural tocopherol level in sunflower oil.
Background technology
Natural VE (VitaminE) is a kind of liposoluble vitamin, also known as tocopherol, is one of topmost antioxidant.Be dissolved in the organic solvents such as fat and ethanol, water insoluble, heat, acid are stablized, unstable to alkali, responsive to oxygen, insensitive to heat, but time fried, Vitamin E activity obviously reduces.Tocopherol can promote that sex hormone is secreted, and man's sperm motility and quantity are increased; Woman's female hormone concentration is increased, and improve fecundity, prevention of miscarriage, also can be used for preventing and treating the aspects such as male sterility, burn, frostbite, capillary hemorrhage, climacteric syndrome, beauty treatment has good curative effect.Also found that vitamin E can suppress the lipid peroxidation in eye lens to react, and made end slightly blood vessel dilatation, improves blood circulation in recent years.
Each vegetable oil is one of main source obtaining natural VE in people's daily life, in the vegetable oil of different cultivars, the content of natural VE has larger difference, and therefore the composition of natural VE and content can as of edible vegetable oil and fat quality important evaluation indexes.
Sunflower oil is the oil extracted from sunflower seeds, and because it is nutritious, a lot of national people like to eat in the world, some titles it be healthy oil, oil of lengthening the life.
Sunflower oil is with the famous health-care edible oil of the linoleic acid of high-load.In 21 century, cardiovascular and cerebrovascular disease such as the diseases such as coronary heart disease, headstroke, cerebral thrombus, artery sclerosis, hypertension increase, and long-term edible suitable oil and fat product, can have greatly improved to above-mentioned symptom.Sunflower oil is exactly one very outstanding in healthy grease, and the many local sunflower oils in the present world have become the first-selection oil of consumer and cook, and sunflower oil consumption figure worldwide ranks the 3rd in all vegetable oil.
Sunflower oil contains the multiple materials useful to the mankind such as sterol, vitamin, linoleic acid, and wherein natural tocopherol level content in all main plant oil is the highest; And linoleic acid content can reach about 70%.Sunflower oil can reduce cholesterol in serum level, reduces triglyceride levels, has the effect reduced blood pressure.And sunflower oil light transparent, culinary art time can retain wholefood local flavor, its smoke point is also very high, can exempt the harm of oil smoke to human body.
At present, the analytical approach of vitamin E comprises high performance liquid chromatography, vapor-phase chromatography, thin-layered chromatography, paper chromatography, column chromatography, colourimetry etc., and the most frequently used is high performance liquid chromatography, and China's existing national standards, industry standard all adopt this method.These standards all have employed method for saponification and extract before liquid-phase chromatographic analysis, and the method time is long and step is complicated.
Summary of the invention
The object of the invention is to now provide a kind of assay method detecting natural tocopherol level in sunflower oil, the extraction conditions of ultrasonic method to natural VE is adopted to be optimized, and adopt the content of natural VE in high effective liquid chromatography for measuring sunflower oil sample, for Vegetable Oil Quality standard provides reference, step of the present invention is simple, economical and practical, be suitable for promoting.
For realizing the object of the invention, the technical scheme of employing is:
Detect an assay method for natural tocopherol level in sunflower oil, it is characterized in that comprising the following steps:
(1) take: count by weight, get 1 part, sunflower oil sample, accurately weighed;
(2) ultrasonic extraction: add 2 ~ 3 parts of extracted by ether agent, ultrasonic extraction 20 ~ 25min, pours out collection by supernatant;
(3) step (2) 1 ~ 2 times are repeated;
(4) concentrated: by supernatant collection, to be concentrated into dry, to obtain sample extracting solution;
(5) measure: high-performance liquid chromatogram determination, adopt C18 chromatographic column, column temperature controls at 32 ~ 34 DEG C, mobile phase and water volume ratio are 95:1.8 ~ 2.0, isocratic elution 16 ~ 18min, and flow control is at 1.1 ~ 1.3mL/min, diode array detector determined wavelength 295nm, sample size 20 μ L;
The preparation of reference substance solution: get vitamin E reference substance, makes the reference substance solution of every 1ml containing 3 μ g, 6 μ g, 12 μ g, 30 μ g, 60 μ g;
High performance liquid chromatography: the reference substance solution of variable concentrations is respectively with 20 μ L sample introductions, record the peak area of vitamin E under 295nm wavelength, with each standard reference material peak area of gained for ordinate, each corresponding standard reference material concentration is that horizontal ordinate does typical curve respectively, calculates the equation of linear regression of vitamin E respectively; Then high-performance liquid chromatogram determination is carried out to sample extraction liquor, obtain the peak area of vitamin E under 295nm wavelength, according to the content of vitamin E in respective typical curve calculation sample extract, and in conjunction with the quality of sunflower oil sample, calculate the content of vitamin E.
Further, the middle flow control of described step (5) is at 1.2mL/min.
Further, described step (5) center pillar temperature control is at 33 DEG C.
The invention has the beneficial effects as follows:
One, ultrasound wave extract yield of the present invention is high, measures relative deviation little, has good accuracy and precision to the mensuration of vitamin E.
Two, step of the present invention is comparatively simple, and adopt compared with saponification in the world, and contracting ovum detection test specimen, is more suitable for grain and oil relevant enterprise and carries out qualitative control greatly.
Embodiment
Be described in further detail below by specific embodiment.
Embodiment 1
Detect an assay method for natural tocopherol level in sunflower oil, comprise the following steps:
(1) take: count by weight, get 1 part, sunflower oil sample, accurately weighed;
(2) ultrasonic extraction: add 2 ~ 3 parts of extracted by ether agent, ultrasonic extraction 20 ~ 25min, pours out collection by supernatant;
(3) step (2) 2 times are repeated;
(4) concentrated: by supernatant collection, to be concentrated into dry, to obtain sample extracting solution;
(5) measure: high-performance liquid chromatogram determination, adopt C18 chromatographic column, column temperature controls at 33 DEG C, and mobile phase and water volume ratio are 95:2, isocratic elution 18min, flow control at 1.2mL/min, diode array detector determined wavelength 295nm, sample size 20 μ L;
The preparation of reference substance solution: get vitamin E reference substance, makes the reference substance solution of every 1ml containing 3 μ g, 6 μ g, 12 μ g, 30 μ g, 60 μ g;
High performance liquid chromatography: the reference substance solution of variable concentrations is respectively with 20 μ L sample introductions, record the peak area of vitamin E under 295nm wavelength, with each standard reference material peak area of gained for ordinate, each corresponding standard reference material concentration is that horizontal ordinate does typical curve respectively, calculates the equation of linear regression of vitamin E respectively; Then high-performance liquid chromatogram determination is carried out to sample extraction liquor, obtain the peak area of vitamin E under 295nm wavelength, according to the content of vitamin E in respective typical curve calculation sample extract, and in conjunction with the quality of sunflower oil sample, calculate the content of vitamin E.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. detect an assay method for natural tocopherol level in sunflower oil, it is characterized in that comprising the following steps:
(1) take: count by weight, get 1 part, sunflower oil sample, accurately weighed;
(2) ultrasonic extraction: add 2 ~ 3 parts of extracted by ether agent, ultrasonic extraction 20 ~ 25min, pours out collection by supernatant;
(3) step (2) 1 ~ 2 times are repeated;
(4) concentrated: by supernatant collection, to be concentrated into dry, to obtain sample extracting solution;
(5) measure: high-performance liquid chromatogram determination, adopt C18 chromatographic column, column temperature controls at 32 ~ 34 DEG C, mobile phase and water volume ratio are 95:1.8 ~ 2.0, isocratic elution 16 ~ 18min, and flow control is at 1.1 ~ 1.3mL/min, diode array detector determined wavelength 295nm, sample size 20 μ L;
The preparation of reference substance solution: get vitamin E reference substance, makes the reference substance solution of every 1ml containing 3 μ g, 6 μ g, 12 μ g, 30 μ g, 60 μ g;
High performance liquid chromatography: the reference substance solution of variable concentrations is respectively with 20 μ L sample introductions, record the peak area of vitamin E under 295nm wavelength, with each standard reference material peak area of gained for ordinate, each corresponding standard reference material concentration is that horizontal ordinate does typical curve respectively, calculates the equation of linear regression of vitamin E respectively; Then high-performance liquid chromatogram determination is carried out to sample extraction liquor, obtain the peak area of vitamin E under 295nm wavelength, according to the content of vitamin E in respective typical curve calculation sample extract, and in conjunction with the quality of sunflower oil sample, calculate the content of vitamin E.
2. a kind of assay method detecting natural tocopherol level in sunflower oil according to claim 1, is characterized in that: in described step (5), flow control is at 1.2mL/min.
3. a kind of assay method detecting natural tocopherol level in sunflower oil according to claim 1, is characterized in that: described step (5) center pillar temperature control is at 33 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510565653.9A CN105136932A (en) | 2015-09-08 | 2015-09-08 | Determination method of natural vitamin E content in sunflower oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510565653.9A CN105136932A (en) | 2015-09-08 | 2015-09-08 | Determination method of natural vitamin E content in sunflower oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105136932A true CN105136932A (en) | 2015-12-09 |
Family
ID=54722357
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510565653.9A Pending CN105136932A (en) | 2015-09-08 | 2015-09-08 | Determination method of natural vitamin E content in sunflower oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105136932A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018647A (en) * | 2016-07-11 | 2016-10-12 | 吉林益民堂制药有限公司 | Method for establishing sunflower plate HPLC standard fingerprint |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090044A (en) * | 2014-07-16 | 2014-10-08 | 中国农业科学院油料作物研究所 | Method for analyzing fatty acid composition in edible vegetable oil and true and false identification method of edible vegetable oil based on fatty acid composition |
| CN104569262A (en) * | 2014-12-30 | 2015-04-29 | 广州白云山汉方现代药业有限公司 | Method for simultaneously determining contents of alpha-tocopherol and squalene in multiple vegetable oils |
-
2015
- 2015-09-08 CN CN201510565653.9A patent/CN105136932A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090044A (en) * | 2014-07-16 | 2014-10-08 | 中国农业科学院油料作物研究所 | Method for analyzing fatty acid composition in edible vegetable oil and true and false identification method of edible vegetable oil based on fatty acid composition |
| CN104569262A (en) * | 2014-12-30 | 2015-04-29 | 广州白云山汉方现代药业有限公司 | Method for simultaneously determining contents of alpha-tocopherol and squalene in multiple vegetable oils |
Non-Patent Citations (3)
| Title |
|---|
| 李柰等: "食用植物油中生育酚含量测定方法优化及分析", 《食品科学技术学报》 * |
| 牟德海等: "柑桔皮精油中β-胡萝卜素、维生素E和硒含量的测定", 《分析测试学报》 * |
| 黄宏南等: "特殊植物油中维生素E的HPLC分析", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018647A (en) * | 2016-07-11 | 2016-10-12 | 吉林益民堂制药有限公司 | Method for establishing sunflower plate HPLC standard fingerprint |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Simon et al. | Serum fatty acids and the risk of stroke | |
| Jarret et al. | Seed oil and fatty acid content in okra (Abelmoschus esculentus) and related species | |
| Chen et al. | Simultaneous extraction and separation of oil, proteins, and glucosinolates from Moringa oleifera seeds | |
| Andersson et al. | Carotenoids in sea buckthorn (Hippophae rhamnoides L.) berries during ripening and use of pheophytin a as a maturity marker | |
| Vásquez-Caicedo et al. | Accumulation of all-trans-β-carotene and its 9-cis and 13-cis stereoisomers during postharvest ripening of nine Thai mango cultivars | |
| Salvatore et al. | Antioxidant characterization of some Sicilian edible wild greens | |
| Chen et al. | Analytical characterization of hempseed (seed of Cannabis sativa L.) oil from eight regions in China | |
| Beveridge et al. | Processing effects on the composition of sea buckthorn juice from Hippophae rhamnoides L. Cv. Indian Summer | |
| Shin et al. | Oxidative stability and quality characteristics of duck, chicken, swine and bovine skin fats extracted by pressurized hot water extraction | |
| Li et al. | Solubility and physicochemical properties of resveratrol in peanut oil | |
| Zhang et al. | Chemical composition of 13 commercial soybean samples and their antioxidant and anti-inflammatory properties | |
| Ahmed et al. | Determination of tea saponin in Camellia seed oil with UV and HPLC analysis | |
| CN102565270B (en) | Method for testing 3-chlorine-1, 2-propylene glycol and aliphatic ester content of 3-chlorine-1, 2-propylene glycol | |
| Nowak et al. | Effect of chokeberry juice consumption on antioxidant capacity, lipids profile and endothelial function in healthy people: a pilot study. | |
| CN105116073A (en) | Determination method for detecting content of natural vitamin E in palm oil | |
| Huang et al. | Simultaneous determination of α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, sesamin, sesamolin, sesamol, and asarinin in sesame oil by normal-phase high performance liquid chromatography | |
| Hajib et al. | Oil content, lipid profiling and oxidative stability of “Sefri” Moroccan pomegranate (Punica granatum L.) seed oil | |
| Singanusong et al. | Effects of Maturity on Chemical Composition and Antioxidant Activity of Sacha Inchi (Plukenetia volubilis L.) Cultivated in NorthernThailand | |
| CN103940654A (en) | Method for extracting vitamin E from waste kaolin and thin layer chromatography detection method | |
| Hamacek et al. | Murici, fruit from the Cerrado of Minas Gerais, Brazil: physical and physicochemical characteristics, and occurrence and concentration of carotenoids and vitamins | |
| CN105136932A (en) | Determination method of natural vitamin E content in sunflower oil | |
| Celik et al. | A rapid validated HPLC method for determination of sulforaphane and glucoraphanin in broccoli and red cabbage prepared by various cooking techniques | |
| Jin et al. | Anti-inflammatory activity of 6-hydroxy-2, 7-dimethoxy-1, 4-henanthraquinone from tuberous roots of yam (Dioscorea batatas) through inhibition of prostaglandin D 2 and leukotriene C 4 production in mouse bone marrow-derived mast cells | |
| CN105116079A (en) | Determining method for detecting content of natural vitamin E in peanut oil | |
| CN105044256A (en) | Measuring method for detecting content of natural vitamins E in corn oil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151209 |
|
| RJ01 | Rejection of invention patent application after publication |