CN105116079A - Determining method for detecting content of natural vitamin E in peanut oil - Google Patents
Determining method for detecting content of natural vitamin E in peanut oil Download PDFInfo
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- CN105116079A CN105116079A CN201510565654.3A CN201510565654A CN105116079A CN 105116079 A CN105116079 A CN 105116079A CN 201510565654 A CN201510565654 A CN 201510565654A CN 105116079 A CN105116079 A CN 105116079A
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Abstract
The invention relates to the field of determining the content of natural products and in particular relates to a determining method for detecting the content of natural vitamin E in peanut oil. According to the method, an ultrasonic method is adopted for optimizing extraction conditions of the natural vitamin E, and a high efficiency liquid chromatography is adopted for determining the content of the natural vitamin E in a peanut oil sample, so that a reference is provided for vegetable oil quality standards. The method is simple in step, is economical and practical and is suitable for being generalized.
Description
Technical field
The present invention relates to natural products assay field, be specially a kind of assay method detecting natural tocopherol level in peanut oil.
Background technology
Natural VE (VitaminE) is a kind of liposoluble vitamin, also known as tocopherol, is one of topmost antioxidant.Be dissolved in the organic solvents such as fat and ethanol, water insoluble, heat, acid are stablized, unstable to alkali, responsive to oxygen, insensitive to heat, but time fried, Vitamin E activity obviously reduces.Tocopherol can promote that sex hormone is secreted, and man's sperm motility and quantity are increased; Woman's female hormone concentration is increased, and improve fecundity, prevention of miscarriage, also can be used for preventing and treating the aspects such as male sterility, burn, frostbite, capillary hemorrhage, climacteric syndrome, beauty treatment has good curative effect.Also found that vitamin E can suppress the lipid peroxidation in eye lens to react, and made end slightly blood vessel dilatation, improves blood circulation in recent years.
Each vegetable oil is one of main source obtaining natural VE in people's daily life, in the vegetable oil of different cultivars, the content of natural VE has larger difference, and therefore the composition of natural VE and content can as of edible vegetable oil and fat quality important evaluation indexes.
Peanut oil (peanutoil) is yellowish transparent, and color and luster is limpid, fragrant, and flavour is good to eat, is the edible oil that a kind of ratio is easier to digest.Peanut oil is containing unsaturated fatty acid more than 80% (wherein containing oleic acid 41.2%, linoleic acid 37.6%).In addition also containing palmitic acid, the saturated fatty acid such as stearic acid and arachidic acid 19.9%.The Fatty acid compositions of peanut oil is reasonable, is easy to human consumption and absorbs.According to foreign data introduction, use peanut oil, cholesterol in human body can be made to be decomposed into bile acid and to excrete, thus reduce the content of cholesterol in blood plasma.Go up in addition, also containing the material that sterol, wheat germ phenol, phosphatide, vitamin E, choline etc. are useful to human body in peanut oil.Frequent edible peanut oil, can prevent wrinkled skin aging, and protection vascular wall, prevents thrombosis, contributes to prevention of arterial sclerosis and coronary heart disease.Choline in peanut oil, also can improve the memory of human brain, delay brain function decline.
The fatty acid composition of peanut oil mainly contains palmitic acid (Palmiticacid), stearic acid (Stearicacid), linoleic acid (Linoleicacid), arachidic acid (Arachidicacid) behenic acid (Behenicacid), oleic acid (Oleicacid), eicosenoic acid (Eicosenoicacid), lignoceric acid (Lignocericacid) etc.Also taste composition is smelt containing special: aldehyde (Hexanal) in peanut oil, gamma-butyrolacton (γ-Butyrolactone), aldehyde C-9 (Nonanal), benzaldehyde (Benzaldehyde), phenmethylol (Benzylalcohol), 2 methoxy 3 isopropyl pyrazine (2-Methoxy-3-Isopropylpyrazine), fragrance ingredient: 1, 2, 3-trimethyl cyclopentane (1, 2, 3-Trimethylcyclopentane), 1-ethyl-3-methyl cyclopentane (1-Ethyl-3-Methylcyclopentane), 4-ethyl-2-metoxyphenol (4-Ethyl-2-Methoxyphenol), 4-metoxyphenol (4-Methoxyphenol), 3-(1, 1-dimethyl ethyl) phenol [3-(1, 1-Dimethylethyl) Phenol], 2, 3, 5-trimethylpyrazine (2, 3, 5-Trimethylpyrazine), 2-methyl-5-propenyl pyrazine (2-Methyl-5-Propenylpyrazine), methylfuroate (Methylfuroate), 3, 5-diethyl-2-methylpyrazine (3, 5-Diethyl-2-Methylpyrazine), 2-acetyl pyrrole [1-(1H-Pyrrole-2-yl) Ethanone], 2, 4-dimethylthiazole (2, 4-Dimethylthiazole), 2, 5-dimethylthiazole (2, 5-Dimethylthiazole), 5-methyl-2-furfural (5-Methyl-2-Furfural) 2-is base furans (2-Hexylfuran), 3-is base furans (3-Hexylfuran), 2-acetyl group-5-methylfuran (2-Acetyl-5-Methylfuran), 2, 3-Dihydrobenzofuranes (2, 3-Dihydrobenzofuran).The total amount of fragrance ingredient is about 19.77mg/kg, measuring main composition is pyrazine compounds, content 12.33mg/kg, account for 61.81% of peanut oil fragrance ingredient total amount, wherein 2,6-dimethyl pyrazines (2,6-Dimethylpyrazine) are the most important, content is 3.92mg/kg, accounts for 19.83% of fragrance ingredient total amount; Secondary composition is furan compound, and wherein 2,3-Dihydrobenzofuranes content are 1.61mg/kg, account for 8.14% of fragrance ingredient total amount.Another containing vitamin (Vitamin) E.
At present, the analytical approach of vitamin E comprises high performance liquid chromatography, vapor-phase chromatography, thin-layered chromatography, paper chromatography, column chromatography, colourimetry etc., and the most frequently used is high performance liquid chromatography, and China's existing national standards, industry standard all adopt this method.These standards all have employed method for saponification and extract before liquid-phase chromatographic analysis, and the method time is long and step is complicated.
Summary of the invention
The object of the invention is to now provide a kind of assay method detecting natural tocopherol level in peanut oil, the extraction conditions of ultrasonic method to natural VE is adopted to be optimized, and adopt the content of natural VE in high effective liquid chromatography for measuring peanut oil sample, for Vegetable Oil Quality standard provides reference, step of the present invention is simple, economical and practical, be suitable for promoting.
For realizing the object of the invention, the technical scheme of employing is:
Detect an assay method for natural tocopherol level in peanut oil, it is characterized in that comprising the following steps:
(1) take: count by weight, get 1 part, peanut oil sample, accurately weighed;
(2) ultrasonic extraction: add 3 ~ 4 parts of methylene chloride-normal hexane mixing extraction agent, ultrasonic extraction 20 ~ 25min, pours out collection by supernatant;
(3) step (2) 1 ~ 2 times are repeated;
(4) concentrated: by supernatant collection, to be concentrated into dry, to obtain sample extracting solution;
(5) measure: high-performance liquid chromatogram determination, adopt C18 chromatographic column, column temperature controls at 32 ~ 34 DEG C, mobile phase and water volume ratio are 95:1.8 ~ 2.0, isocratic elution 16 ~ 18min, and flow control is at 1.1 ~ 1.3mL/min, diode array detector determined wavelength 295nm, sample size 20 μ L;
The preparation of reference substance solution: get vitamin E reference substance, makes the reference substance solution of every 1ml containing 3 μ g, 6 μ g, 12 μ g, 30 μ g, 60 μ g;
High performance liquid chromatography: the reference substance solution of variable concentrations is respectively with 20 μ L sample introductions, record the peak area of vitamin E under 295nm wavelength, with each standard reference material peak area of gained for ordinate, each corresponding standard reference material concentration is that horizontal ordinate does typical curve respectively, calculates the equation of linear regression of vitamin E respectively; Then high-performance liquid chromatogram determination is carried out to sample extraction liquor, obtain the peak area of vitamin E under 295nm wavelength, according to the content of vitamin E in respective typical curve calculation sample extract, and in conjunction with the quality of peanut oil sample, calculate the content of vitamin E.
Further, in described step (2) methylene chloride-normal hexane mixing extraction agent, the mass ratio of methylene chloride and normal hexane is 8:(3 ~ 4).
Further, the middle flow control of described step (5) is at 1.2mL/min.
Further, described step (5) center pillar temperature control is at 33 DEG C.
The invention has the beneficial effects as follows:
One, ultrasound wave extract yield of the present invention is high, measures relative deviation little, has good accuracy and precision to the mensuration of vitamin E.
Two, step of the present invention is comparatively simple, and adopt compared with saponification in the world, and contracting ovum detection test specimen, is more suitable for grain and oil relevant enterprise and carries out qualitative control greatly.
Embodiment
Be described in further detail below by specific embodiment.
Embodiment 1
Detect an assay method for natural tocopherol level in peanut oil, comprise the following steps:
(1) take: count by weight, get 1 part, peanut oil sample, accurately weighed;
(2) ultrasonic extraction: add 3 ~ 4 parts of methylene chloride-normal hexane mixing extraction agent (mass ratio of methylene chloride and normal hexane is 8:3), ultrasonic extraction 20 ~ 25min, pours out collection by supernatant;
(3) step (2) 2 times are repeated;
(4) concentrated: by supernatant collection, to be concentrated into dry, to obtain sample extracting solution;
(5) measure: high-performance liquid chromatogram determination, adopt C18 chromatographic column, column temperature controls at 33 DEG C, and mobile phase and water volume ratio are 95:2, isocratic elution 18min, flow control at 1.2mL/min, diode array detector determined wavelength 295nm, sample size 20 μ L;
The preparation of reference substance solution: get vitamin E reference substance, makes the reference substance solution of every 1ml containing 3 μ g, 6 μ g, 12 μ g, 30 μ g, 60 μ g;
High performance liquid chromatography: the reference substance solution of variable concentrations is respectively with 20 μ L sample introductions, record the peak area of vitamin E under 295nm wavelength, with each standard reference material peak area of gained for ordinate, each corresponding standard reference material concentration is that horizontal ordinate does typical curve respectively, calculates the equation of linear regression of vitamin E respectively; Then high-performance liquid chromatogram determination is carried out to sample extraction liquor, obtain the peak area of vitamin E under 295nm wavelength, according to the content of vitamin E in respective typical curve calculation sample extract, and in conjunction with the quality of peanut oil sample, calculate the content of vitamin E.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. detect an assay method for natural tocopherol level in peanut oil, it is characterized in that comprising the following steps:
(1) take: count by weight, get 1 part, peanut oil sample, accurately weighed;
(2) ultrasonic extraction: add 3 ~ 4 parts of methylene chloride-normal hexane mixing extraction agent, ultrasonic extraction 20 ~ 25min, pours out collection by supernatant;
(3) step (2) 1 ~ 2 times are repeated;
(4) concentrated: by supernatant collection, to be concentrated into dry, to obtain sample extracting solution;
(5) measure: high-performance liquid chromatogram determination, adopt C18 chromatographic column, column temperature controls at 32 ~ 34 DEG C, mobile phase and water volume ratio are 95:1.8 ~ 2.0, isocratic elution 16 ~ 18min, and flow control is at 1.1 ~ 1.3mL/min, diode array detector determined wavelength 295nm, sample size 20 μ L;
The preparation of reference substance solution: get vitamin E reference substance, makes the reference substance solution of every 1ml containing 3 μ g, 6 μ g, 12 μ g, 30 μ g, 60 μ g;
High performance liquid chromatography: the reference substance solution of variable concentrations is respectively with 20 μ L sample introductions, record the peak area of vitamin E under 295nm wavelength, with each standard reference material peak area of gained for ordinate, each corresponding standard reference material concentration is that horizontal ordinate does typical curve respectively, calculates the equation of linear regression of vitamin E respectively; Then high-performance liquid chromatogram determination is carried out to sample extraction liquor, obtain the peak area of vitamin E under 295nm wavelength, according to the content of vitamin E in respective typical curve calculation sample extract, and in conjunction with the quality of peanut oil sample, calculate the content of vitamin E.
2. a kind of assay method detecting natural tocopherol level in peanut oil according to claim 1, is characterized in that: in described step (2) methylene chloride-normal hexane mixing extraction agent, the mass ratio of methylene chloride and normal hexane is 8:(3 ~ 4).
3. a kind of assay method detecting natural tocopherol level in peanut oil according to claim 1, is characterized in that: in described step (5), flow control is at 1.2mL/min.
4. a kind of assay method detecting natural tocopherol level in peanut oil according to claim 1, is characterized in that: described step (5) center pillar temperature control is at 33 DEG C.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018166342A1 (en) * | 2017-03-13 | 2018-09-20 | 上海融氏健康产业股份有限公司 | Method for evaluating freshness of oil/fat and device for calculating freshness of oil/fat |
| CN112067714A (en) * | 2020-08-26 | 2020-12-11 | 青岛市食品药品检验研究院（青岛市药品不良反应监测中心、青岛市实验动物和动物实验中心） | Method for determining peanut oil essence in peanut oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012099037A1 (en) * | 2011-01-17 | 2012-07-26 | 築野食品工業株式会社 | Analytical method for vitamin e |
| CN104569262A (en) * | 2014-12-30 | 2015-04-29 | 广州白云山汉方现代药业有限公司 | Method for simultaneously determining contents of alpha-tocopherol and squalene in multiple vegetable oils |
-
2015
- 2015-09-08 CN CN201510565654.3A patent/CN105116079A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012099037A1 (en) * | 2011-01-17 | 2012-07-26 | 築野食品工業株式会社 | Analytical method for vitamin e |
| CN104569262A (en) * | 2014-12-30 | 2015-04-29 | 广州白云山汉方现代药业有限公司 | Method for simultaneously determining contents of alpha-tocopherol and squalene in multiple vegetable oils |
Non-Patent Citations (10)
| Title |
|---|
| Analysis of carotenoids and vitamin E in selected oilseeds,press cakes and oils;Saskia Franke等;《Eur. J. Lipid Sci. Technol.》;20100920;第112卷(第10期);第1122-1129页 * |
| DLPME- HPLC 同时测定微量人血清中的维生素 A 和 E;郑凤家等;《现代预防医学》;20110425;第38卷(第8期);第1523页第2.1节 * |
| SASKIA FRANKE等: "Analysis of carotenoids and vitamin E in selected oilseeds,press cakes and oils", 《EUR. J. LIPID SCI. TECHNOL.》 * |
| 何伟: "反相液相色谱法测定植物油中VE含量的方法研究", 《中国优秀硕士学位论文全文数据库工程科技I辑》 * |
| 刁全平等: "高效液相色谱法测定深海鱼油中维生素 E 的含量", 《鞍山师范学院学报》 * |
| 反相液相色谱法测定植物油中VE含量的方法研究;何伟;《中国优秀硕士学位论文全文数据库工程科技I辑》;20120415(第4期);第14-17页第1-2节，第27页第1.2节和第33-35页第1-2节 * |
| 反相高效液相色谱法快速测定植物油中的维生素E;寇立娟等;《中国食物与营养》;20061231(第12期);第42-43页 * |
| 寇立娟等: "反相高效液相色谱法快速测定植物油中的维生素E", 《中国食物与营养》 * |
| 郑凤家等: "DLPME- HPLC 同时测定微量人血清中的维生素 A 和 E", 《现代预防医学》 * |
| 高效液相色谱法测定深海鱼油中维生素 E 的含量;刁全平等;《鞍山师范学院学报》;20120831;第14卷(第4期);摘要部分 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018166342A1 (en) * | 2017-03-13 | 2018-09-20 | 上海融氏健康产业股份有限公司 | Method for evaluating freshness of oil/fat and device for calculating freshness of oil/fat |
| CN112067714A (en) * | 2020-08-26 | 2020-12-11 | 青岛市食品药品检验研究院（青岛市药品不良反应监测中心、青岛市实验动物和动物实验中心） | Method for determining peanut oil essence in peanut oil |
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Application publication date: 20151202 |