CN105136790B - 一种牛肉汞含量的检测方法 - Google Patents

一种牛肉汞含量的检测方法 Download PDF

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CN105136790B
CN105136790B CN201510565617.2A CN201510565617A CN105136790B CN 105136790 B CN105136790 B CN 105136790B CN 201510565617 A CN201510565617 A CN 201510565617A CN 105136790 B CN105136790 B CN 105136790B
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CN105136790A (zh
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陈喜良
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Da manor meat Group Co.,Ltd.
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Henan Grand Farm Meat Industry Co Ltd
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Abstract

本发明公开了一种牛肉汞含量的检测方法，包括以下步骤：取二苯碳酰二肼乙醚溶液与乳化剂OP水溶液按比例混合，中速定量滤纸浸渍，制成试纸；取1000μg/mL标准汞溶液，HCl溶液调节pH至6.0，加水稀释汞含量为0.5μg/mL的溶液，放入试纸，显色，拍照，处理后形成纸标准色阶。称取牛肉样品，破碎，加10％HCl，微波消解，离心，取上清液，调整PH值在6.0，减压浓缩上清液至牛肉样品重量的1/10，放入试纸，显色，拍照，将试纸显色深度和标准色阶比较，判断汞含量是否超标。发明的检测方法操作简单灵敏度高，作为汞含量是否超标的初筛，适合工厂生产质检的使用。

Description

一种牛肉汞含量的检测方法
技术领域
本发明涉及肉制品重金属检验领域，具体为一种牛肉汞含量的检测方法。
背景技术
牛肉是全世界人都爱吃的食品，中国人消费的肉类食品之一，仅次于猪肉，它含有丰富的蛋白质，氨基酸组成比猪肉更接近人体需要，能提高机体抗病能力，寒冬食牛肉，有暖胃作用，为寒冬补益佳品。中医认为，牛肉有补中益气、滋养脾胃、强健筋骨、化痰息风、止渴止涎的功效。适用于中气下陷、气短体虚，筋骨酸软、贫血久病及面黄目眩之人食用。
随着人们对牛肉在需求量的增大，肉牛的养殖和屠宰业得以迅速的发展，由于养殖户的大小不一，加之环境的污染，使得屠宰企业的出产的肉制品的质量很不稳定。每个肉制品生成企业都在积极摸索和建立自己的检测系统，力求保障自己产品的安全，在牛肉制品的国家卫生标准中，对汞含量有严格的限定，牛肉中的汞含量不大于0.05mg/KG，因此对汞含量的检测尤为重要。
发明内容
本发明的目的是提供一种牛肉汞含量的检测方法，该发明的检测方法操作简单，适合工厂生产质检的使用。
本发明的目的是通过以下技术方案来实现的：
本发明的一种牛肉汞含量的检测方法，包括以下步骤：
(1)制备检测试纸：
取0.7％二苯碳酰二肼乙醚溶液与15％乳化剂OP水溶液按6∶1体积比混合均匀，加入中速定量滤纸浸渍15min，取出，避光、自然晾干，制成二苯碳酰二肼试纸，避光保存；
(2)制备汞含量为0.5mg/KG的试纸色卡：
取1000μg/mL标准汞溶液适量，加水适量并以HCl溶液调节pH至6.0，加水稀释并制成汞含量为0.5μg/mL的溶液作为标准汞对比溶液；取二苯碳酰二肼试纸，置于上述标准对比溶液中，计时5min，取出，置于白色水平背景上，立即拍照；照片经“画图”程序处理后形成二苯碳酰二肼纸标准色阶；
(3)称取牛肉样品，破碎，加入3倍量的10％HCl，微波消解，离心，取上清液，使用HCl和NaOH调整pH值在6.0，减压浓缩上清液至牛肉样品重量的1/10；
(4)将制备好的检测试纸放入步骤(2)中的浓缩后的上清液中，5min后，观察显色情况，拍照；
(5)将牛肉样品上清液的试纸显色深度和标准色阶比较，若牛肉样品上清液的试纸显色深度明显小于标准色阶，则判断为所抽检牛肉的汞含量低于国家标准；若牛肉样品上清液的试纸显色深度相当或大于标准色阶，则判断所抽检牛肉的汞含量有问题，继续使用更为精密的检测方法再行检测汞含量，判断汞含量是否超标。
优选的，所述步骤(1)中，二苯碳酰二肼试纸裁剪成1cm×1cm的小方片。
本发明的优点：
1.该发明的检测方法操作简单，适合工厂生产质检的使用。
2.该发明的检测方法，灵敏度高，作为汞含量是否超标的初筛。
具体实施方式
以下结合具体实施例对本发明作进一步说明：
实施例
制备检测试纸，供日常抽检检测使用：
取0.7％二苯碳酰二肼乙醚溶液与15％乳化剂OP水溶液按6∶1体积比混合均匀，加入中速定量滤纸浸渍15min，取出，避光、自然晾干，制成二苯碳酰二肼试纸，避光保存；
为了方便使用，可以将试纸裁剪成1cm×1cm的小方片；
通常根据5个月的试纸使用量来制备一次试纸。
制备汞含量为0.5mg/KG的试纸色卡：
取1000μg/mL标准汞溶液适量，加水适量并以HCl溶液调节pH至6.0，加水稀释并制成汞含量为0.5μg/mL的溶液作为标准汞对比溶液；取二苯碳酰二肼试纸，置于上述标准对比溶液中，计时5min，取出，置于白色水平背景上，立即拍照。照片经“画图”程序处理后形成二苯碳酰二肼纸标准色阶，保持标准色阶，供长期检测实验对比使用。
牛肉抽检检测实验：
(1)称取牛肉样品100g，破碎，加入3倍量的10％HCl，微波消解，离心，取上清液，使用HCl和NaOH调整pH值在6.0，减压浓缩上清液至10mL；为了更好取得显色效果，特将标准色阶的汞含量浓度设定为0.5μg/mL，相对于国标中规定的牛肉汞含量标准的10倍，提高汞含量的浓度，显色效果易于观察；同时在取样提取检测液时，也将检测液浓缩至10mL，与标准色阶的汞含量浓度相对应；
(2)将制备好的检测试纸放入步骤(1)中的浓缩后的上清液中，5min后，观察显色情况，拍照；
(3)将牛肉样品上清液的试纸显色深度和标准色阶比较，若牛肉样品上清液的试纸显色深度明显小于标准色阶，则判断为所抽检牛肉的汞含量低于国家标准；若牛肉样品上清液的试纸显色深度相当或大于标准色阶，则判断所抽检牛肉的汞含量有问题，继续使用更为精密的检测方法再行检测汞含量，判断汞含量是否超标。

Claims (2)

1.一种牛肉汞含量的检测方法，其特征在于：包括以下步骤：
(1)制备检测试纸：
取0.7％二苯碳酰二肼乙醚溶液与15％乳化剂OP水溶液按6∶1体积比混合均匀，加入中速定量滤纸浸渍15min，取出，避光、自然晾干，制成二苯碳酰二肼试纸，避光保存；
(2)制备汞含量为0.5mg/KG的试纸色卡：
取1000μg/mL标准汞溶液适量，加水适量并以HCl溶液调节pH至6.0，加水稀释并制成汞含量为0.5μg/mL的溶液作为标准汞对比溶液；取二苯碳酰二肼试纸，置于上述标准对比溶液中，计时5min，取出，置于白色水平背景上，立即拍照；照片经“画图”程序处理后形成二苯碳酰二肼纸标准色阶；
(3)称取牛肉样品，破碎，加入3倍量的10％HCl，微波消解，离心，取上清液，使用HCl和NaOH调整pH值在6.0，减压浓缩上清液至牛肉样品重量的1/10；
(4)将制备好的检测试纸放入步骤(2)中的浓缩后的上清液中，5min后，观察显色情况，拍照；
(5)将牛肉样品上清液的试纸显色深度和标准色阶比较，判断汞含量是否超标。
2.根据权利要求1所述的牛肉汞含量的检测方法，其特征在于：所述步骤(1)中，二苯碳酰二肼试纸裁剪成1cm×1cm的小方片。
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CN106338510A (zh) * 2016-08-30 2017-01-18 安徽鑫河清真牛羊肉加工有限公司 一种清真牛羊肉铅含量快速检测方法
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