CN105136787A - Detection reagent for mono-hydroxyphenyl metabolin urine - Google Patents
Detection reagent for mono-hydroxyphenyl metabolin urine Download PDFInfo
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- CN105136787A CN105136787A CN201510539814.7A CN201510539814A CN105136787A CN 105136787 A CN105136787 A CN 105136787A CN 201510539814 A CN201510539814 A CN 201510539814A CN 105136787 A CN105136787 A CN 105136787A
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Abstract
The invention relates to a detection reagent for mono-hydroxyphenyl metabolin urine. The detection reagent is a water solution at least comprising nitric acid, mercurous nitrate and an amino-contained compound. The detection reagent has higher detection accuracy.
Description
Technical field
The present invention relates to single hydroxyl phenolic metabolism thing urine detection reagent.
Background technology
The large basic resource of the air that the mankind depend on for existence, water source and soil three has been subject to pollution in various degree, and the serious prestige of contaminated environment assists the life of the mankind and healthy, and carcinogenic factor is almost omnipresent, and thus cancer morbidity is in rising trend.
Cancer is one of three large diseases threatening human life, and the number of the infected that China is annual according to the statistics made by the departments concerned reaches 3,720,000 people, and on average every 100,000 people just have 285.91 people to suffer from cancer, and its incidence of disease is quite high.Simultaneously, why cancer presents the situation being difficult to cure, mainly lack and effectively make a definite diagnosis means in early days, so that can not treat timely in early days at precancerous stage and morbidity, therefore, once find that cancer patient has been middle and advanced stage mostly, miss golden hour, so early diagnosis just becomes the key improving treatment of cancer rate.But making a definite diagnosis in early days and depending on of cancer effectively detects in early days.Make a general survey of the detection method of cancer both at home and abroad at present, not yet find that there are highly sensitive, high specificity, non-invasi wide spectrum effective ways fast.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of single hydroxyl phenolic metabolism thing urine detection reagent newly, described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl.
Described aromatic ring or the heteroaromatic compounds containing amino-compound with an amino group and an electron-donating group.
Described is containing amino aryl cyclic compound containing amino-compound or containing ammonia nitrogen heteroaromatic ring compounds, described amino group is positioned at the para postion of electron-donating group.
Described aromatic ring is one or more in benzene, naphthalene, anthracene, phenanthrene, pyrene, aphthacene.
Described fragrant heterocycle is pyridine, pyrimidine, pyrazine, pyridazine, triazine, quinoline, quinoxaline, acridine, imidazo [1,2-a] pyridine, imidazo [1,2-a] pyrimidine, pyrroles, furans, thiophene, isoquinoline, coumarone, imidazoles, carbazole, diazole, triazole.
Hg in described detection reagent
+concentration be 50-1000mmol/L.
H in described detection reagent
+concentration be 100-4000mmol/L.
NO in described detection reagent
3 -concentration be 500-3000mmol/L.
Concentration containing amino-compound in described detection reagent is 0.01-500mmol/L.
The detection method of single hydroxyl phenolic metabolism thing, comprises the following steps,
-obtain human urine to be measured;
-reagent will be detected mix with human urine to be measured, obtain mixed liquor,
Described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl;
-judge the color of mixed liquor, when the color of mixed liquor be brick-red brown or rose time, then represent that human urine to be measured contains single hydroxyl phenolic metabolism thing, when the color of mixed liquor is white or faint yellow, then represent human urine to be measured not containing single hydroxyl phenolic metabolism thing.
Be easier to understand the above-mentioned of the application and other features, aspect and advantage with reference to following detailed description.
Embodiment
Unless otherwise defined, all technology used herein and scientific terminology have the identical implication usually understood with one skilled in the art of the present invention.When there is contradiction, be as the criterion with the definition in this instructions.
Term as used herein " by ... preparation " and " comprising " synonym.Term used herein " comprises ", " comprising ", " having ", " containing " or its other distortion any, be intended to cover the comprising of non-exclusionism.Such as, comprise the composition of listed elements, step, method, goods or device and need not be only limitted to those key elements, but other key element of clearly not listing or the intrinsic key element of this kind of composition, step, method, goods or device can be comprised.
Conjunction " by ... composition " get rid of any key element, step or the component do not pointed out.If in claim, this phrase will make claim be closed, make it not comprise material except those materials described, but except relative customary impurities.When phrase " by ... composition " to appear in the clause of claim main body instead of immediately preceding after theme time, it is only limited to the key element described in this clause; Other key element is not excluded outside described claim as a whole.
During the Range Representation that equivalent, concentration or other value or parameter limit with scope, preferable range or a series of upper limit preferred value and lower preferable values, this is appreciated that all scopes specifically disclosing and formed by arbitrary pairing of any range limit or preferred value and any range lower limit or preferred value, no matter and whether this scope separately discloses.Such as, when disclosing scope " 1 to 5 ", described scope should be interpreted as comprising scope " 1 to 4 ", " 1 to 3 ", " 1-2 ", " 1-2 and 4-5 ", " 1-3 and 5 " etc.When numerical range is described in this article, unless otherwise indicated, otherwise this scope intention comprises its end value and all integers within the scope of this and mark.
In addition, the indefinite article " one " before key element of the present invention or component and " one " are to quantitative requirement (i.e. occurrence number) unrestriction of key element or component.Therefore " one " or " one " should be read as and comprise one or at least one, and the key element of singulative or component also comprise plural form, unless the obvious purport of described quantity refers to singulative.
Single hydroxyl phenolic metabolism thing urine detection reagent, described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl.
containing amino-compound
Of the present invention have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, amide group, phenyl, alkyl.
Ar of the present invention refers to that any one has the cyclic group of aromatic character, so Ar of the present invention all selects the ring compound with aromaticity, particularly, Ar can be selected from aromatic rings, fragrant heterocycle.Heteroatoms can select nitrogen-atoms, sulphur atom, oxygen atom.
As the preferred technical scheme of one, described aromatic ring is one or more in benzene, naphthalene, anthracene, phenanthrene, pyrene, aphthacene.
As the preferred technical scheme of one, described fragrant heterocycle is pyridine, pyrimidine, pyrazine, pyridazine, triazine, quinoline, quinoxaline, acridine, imidazo [1,2-a] pyridine, imidazo [1,2-a] pyrimidine, pyrroles, furans, thiophene, isoquinoline, coumarone, imidazoles, carbazole, diazole, triazole.As the preferred technical scheme of one, more select azacyclo-, as pyrrole, pyridine pyrimidine, pyrazine, pyridazine, triazine, quinoline.
Alkyl of the present invention comprises: methyl, ethyl, propyl group, isopropyl, normal-butyl, sec-butyl, isobutyl, the tert-butyl group, n-pentyl, n-hexyl, n-heptyl, n-octyl.
Amide group of the present invention comprises: formyloxy, acetoxyl group, propionyloxy, butyryl acyloxy, 2-methylpropionyloxy, new pentane acyloxy, penta acyloxy, 3-methylbutyryl oxygen base, hexylyloxy.
Phenyl of the present invention comprises: phenyl, o-methyl-phenyl-, an aminomethyl phenyl, p-methylphenyl, adjacent ethylphenyl, an ethylphenyl, to ethylphenyl, adjacent n-pro-pyl phenyl, a n-pro-pyl phenyl, to n-propylbenzene base.
The preferred carbon number of alkoxy of the present invention is that the alkoxy of 1-6 comprises: methoxyl, ethoxy, positive propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy, amoxy, own oxygen base.
Particularly, described one or more of following compound of can being selected from containing amino-compound of the present invention:
O-toluidine, open-chain crown ether, m-toluidine, adjacent formyloxy aniline, to formyloxy aniline, between formyloxy aniline, o-aminoanisole, P-nethoxyaniline, m-anisidine, p-methylphenyl aniline, o ethyl aniline, to ethylaniline, m-ethyl aniline, adjacent acetoxyl group aniline, to acetoxyl group aniline, between acetoxyl group aniline, O-ethoxyl amine, p-ethoxyaniline, m-oxethyl aniline, to ethylphenyl aniline, adjacent propyl group aniline, to propyl group aniline, between propyl group aniline, adjacent propionyloxy aniline, to propionyloxy aniline, between propionyloxy aniline, adjacent propoxyl group aniline, to propoxyl group aniline, between propoxyl group aniline, to propyl group phenylaniline, 3, 3'-dimethylbenzidine, 5-methoxyl-1-anthranylamine, 5-methoxyl-1-pyrene amine, 5-methoxyl-1-aphthacene amine, 7-methoxyl-naphthalidine, 8-methoxyl-naphthalidine, 6-methoxyl-naphthalidine, 5-methoxyl-naphthalidine, 4-methoxyl-naphthalidine, 3-methoxyl-naphthalidine, 2-methoxyl-naphthalidine, 7-methyl isophthalic acid-naphthylamines, 8-methyl isophthalic acid-naphthylamines, 6-methyl isophthalic acid-naphthylamines, 5-methyl isophthalic acid-naphthylamines, 4-methyl isophthalic acid-naphthylamines, 3-methyl isophthalic acid-naphthylamines, 2-methyl isophthalic acid-naphthylamines, 7-propoxyl group-naphthalidine, 8-propoxyl group-naphthalidine, 6-propoxyl group-naphthalidine, 5-propoxyl group-naphthalidine, 4-propoxyl group-naphthalidine, 3-propoxyl group-naphthalidine, 2-propoxyl group-naphthalidine, 7-propyl group-naphthalidine, 8-propyl group-naphthalidine, 6-propyl group-naphthalidine, 5-propyl group-naphthalidine, 4-propyl group-naphthalidine, 3-propyl group-naphthalidine, 2-propyl group-naphthalidine, 7-ethoxy-naphthalidine, 8-ethoxy-naphthalidine, 6-ethoxy-naphthalidine, 5-ethoxy-naphthalidine, 4-ethoxy-naphthalidine, 3-ethoxy-naphthalidine, 2-ethoxy-naphthalidine, 7-ethyl-naphthalidine, 8-ethyl-naphthalidine, 6-ethyl-naphthalidine, 5-ethyl-naphthalidine, 4-ethyl-naphthalidine, 3-ethyl-naphthalidine, 2-ethyl-naphthalidine, 7-formyloxy-naphthalidine, 8-formyloxy-naphthalidine, 6-formyloxy-naphthalidine, 5-formyloxy-naphthalidine, 4-formyloxy-naphthalidine, 3-formyloxy-naphthalidine, 2-formyloxy-naphthalidine, 7-propionyloxy-naphthalidine, 8-propionyloxy-naphthalidine, 6-propionyloxy-naphthalidine, 5-propionyloxy-naphthalidine, 4-propionyloxy-naphthalidine, 3-propionyloxy-naphthalidine, 2-propionyloxy-naphthalidine, 7-acetoxyl group-naphthalidine, 8-acetoxyl group-naphthalidine, 6-acetoxyl group-naphthalidine, 5-acetoxyl group-naphthalidine, 4-acetoxyl group-naphthalidine, 3-acetoxyl group-naphthalidine, 2-acetoxyl group-naphthalidine,
2,4-dimethyl-3-aminopyridine, 2,4-dimethyl-5-aminopyridine, 2-amino-5-picoline, 4-ethoxy-3-aminopyridine, 2-An Ji – 6-picoline, 3-methyl-4-aminopyridine, 4-acetylaminohydroxyphenylarsonic acid PA, 2,4-dimethyl-3-aminoquinoline, 2,4-dimethyl-5-aminoquinoline, 2-amino-5-methylquinoline, 4-ethoxy-3-aminoquinoline, 2-An Ji – 6-methylquinoline, 3-methyl-4-aminoquinoline, 4-acetylaminohydroxyphenylarsonic acid 2-aminoquinoline, 8-amino-2-methyl quinoline, 5-amino-2-methyl quinoline, amino-2, the 6-dimethyl pyrimidines of 4-, 4-amino-6-methoxy pyrimidine, 5-amino-4-methylpyrimidine, amino-5, the 6-dimethoxypyridins of 4-, 2-amino-6-methylpyrazine, 3-Aminopyrazine-2-carboxylate methyl ester, 2-amino-3-methyl pyrazine, 6-methyl-3-amino pyridazine, 2,4-diamido-6-phenyl-1,3,5-triazines, 2-amino-4-methyl-6-methoxyl-1,3,5-triazines, 6-amino-5-methyl-quinoxaline, 6-amino-5-methoxyl quinoxaline, 9-amino-2-methoxyacridine, amino methyl pyrroles, amino methyl imidazo [1,2-a] pyridine, amino methyl imidazo [1,2-a] pyrimidine, aminomethylfurane, amino methyl thiophene, aminomethylisoquinoline, amino methyl coumarone, amino methyl imidazoles, amino methyl carbazole, amino methyl diazole, amino methyl triazole.
Preferably, used in the present invention containing amino-compound be m-oxethyl aniline, to propionyloxy aniline, 6-ethyl-naphthalidine, 3-formyloxy-naphthalidine, 2,4-dimethyl-5-aminoquinoline, 8-amino-2-methyl quinoline, 2,4-dimethyl-5-aminopyridines, 5-amino-4-methylpyrimidine, 4-acetylaminohydroxyphenylarsonic acid PA, 9-amino-2-methoxyacridine, 2-amino-3-methyl pyrazine.More electedly, select the potpourri of formyloxy-naphthalidine and 2,4-dimethyl-5-aminoquinoline, the concentration containing amino-compound in described detection reagent is 0.01-500mmol/L.
chromogenic reagent
Detection reagent of the present invention is for containing H
+, Ni
2+, Hg
+, Hg
2+, NO
3 -the acid solution of ion, H
+concentration be 100-4000mmol/L, Hg in described detection reagent
+concentration be 10-1000mmol/L, NO in described detection reagent
3 -concentration be 500-14000mmol/L, detect in reagent and can also comprise SO
4 2-, described SO
4 2-concentration is 50-3000mmol/L.
Acid solution and single hydroxyl phenolic metabolism thing react, and obtain brick-red or brown or rose reaction product, and when there is not single hydroxyl phenolic metabolism thing, then obtain white or close to yellow or linen reaction product.
Although the phosphomolybdic acid in the present invention can ionize out hydrogen ion in aqueous, but in order to describe the present invention easily, the hydrogen ion that phosphomolybdic acid ionizes out does not count by the hydrionic concentration in the present invention, and is calculated as the polyprotonic acid not occurring to ionize by phosphomolybdic acid.
Phosphomolybdic acid of the present invention can allow chromogenic reaction mode according to expectation carry out effectively, especially when mercurous ion concentration is low.Hg in described detection reagent
+concentration and the concentration ratio of phosphomolybdic acid be 1:0.9-2.Adopt above-mentioned ratio more can have economy, effectively obtain effective Detection results.
other ions
Detection reagent of the present invention can also contain strong acid radical ion or alkali metal ion, and these ions can not affect the use of detection reagent of the present invention, so the present invention detects the various ion (H in reagent
+, Ni
2+, Hg
+, Hg
+, NO
3 -) not by the impact of anion-cation balance, the ratio of various ion can be arbitrary ratio.
detection method
In human urine, the detection method of single hydroxyl phenolic metabolism thing, comprises the following steps,
-obtain human urine to be measured;
-reagent will be detected mix with human urine to be measured, obtain mixed liquor,
Described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl;
-judge the color of mixed liquor, when the color of mixed liquor be brick-red brown or rose time, then represent that human urine to be measured contains single hydroxyl phenolic metabolism thing, when the color of mixed liquor is white, faint yellow, then represent human urine to be measured not containing single hydroxyl phenolic metabolism thing.
Embodiment:
Embodiment 1, single hydroxyl phenolic metabolism thing urine detection reagent, described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl.
Embodiment 2, identical with embodiment 1, difference is, has aromatic ring or the heteroaromatic compounds of an amino group and an electron-donating group containing amino-compound.
Embodiment 3, identical with embodiment 1, difference is, containing amino-compound for containing amino aryl cyclic compound or containing ammonia nitrogen heteroaromatic ring compounds, described amino group is positioned at the para postion of electron-donating group.
Embodiment 4, identical with embodiment 1, difference is, aromatic ring is one or more in benzene, naphthalene, anthracene, phenanthrene, pyrene, aphthacene.
Embodiment 5, identical with embodiment 1, difference is, virtue heterocycle is pyridine, pyrimidine, pyrazine, pyridazine, triazine, quinoline, quinoxaline, acridine, imidazo [1,2-a] pyridine, imidazo [1,2-a] pyrimidine, pyrroles, furans, thiophene, isoquinoline, coumarone, imidazoles, carbazole, diazole, triazole.
Embodiment 6, identical with embodiment 1, difference is, detects Hg in reagent
+concentration be 50-1000mmol/L.
Embodiment 7, identical with embodiment 1, difference is, detects H in reagent
+concentration be 100-4000mmol/L.
Embodiment 8, identical with embodiment 1, difference is, detects NO in reagent
3 -concentration be 500-3000mmol/L.
Embodiment 9, identical with embodiment 1, difference is, the concentration detected containing amino-compound in reagent is 0.01-500mmol/L.
The detection method of embodiment 10. single hydroxyl phenolic metabolism thing, comprises the following steps,
-obtain human urine to be measured;
-reagent will be detected mix with human urine to be measured, obtain mixed liquor,
Described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl;
-judge the color of mixed liquor, when the color of mixed liquor be brick-red brown or rose time, then represent that human urine to be measured contains single hydroxyl phenolic metabolism thing, when the color of mixed liquor is white or faint yellow, then represent human urine to be measured not containing single hydroxyl phenolic metabolism thing.
Hereinafter, by embodiment, the present invention is explained in more detail, but should be understood that these embodiments are only illustrative and nonrestrictive.If do not have other to illustrate, raw materials used is all commercially available.
The present invention is described in detail referring to several example.
Evaluation method:
Artificial urine:
The urine of artificial urine 1(normal person adds Single-chip microcomputer, Single-chip microcomputer content 15mg/L).
The urine of artificial urine 2(normal person adds polypeptide, content of peptides 15mg/L).
The urine of artificial urine 3(normal person adds polypeptide and Single-chip microcomputer, content of peptides 15mg/L, Single-chip microcomputer content 15mg/L).
Wherein, polypeptide is ALA-GLU-1B-TYR polypeptide polymer (147245-92-9).
Embodiment 1
Detect reagent: H
+concentration be 1000mmol/L, Hg
+concentration be 50mmol/L, Ni
2+concentration be 150mmol/L, NO
3 -concentration be 1350mmol/L.M-oxethyl aniline 0.01mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 shows pale red, and artificial urine 2 whitening look, artificial urine 3 shows pale red.
Embodiment 2
Detect reagent: H
+concentration be 100mmol/L, Hg
+concentration be 200mmol/L, NO
3 -concentration be 400mmol/L.To propionyloxy aniline 0.1mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 shows pale red, and artificial urine 2 whitening look, artificial urine 3 shows pale red.
Embodiment 3
Detect reagent: H
+concentration be 2000mmol/L, Hg
+concentration be 500mmol/L, NO
3 -concentration be 2500mmol/L.6-ethyl-naphthalidine 1mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 is aobvious red, artificial urine 2 whitening look, and artificial urine 3 is aobvious red.
Embodiment 4
Detect reagent: H
+concentration be 1000mmol/L, Hg
+concentration be 200mmol/LNO
3 -concentration be 1200mmol/L.3-formyloxy-naphthalidine 10mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Embodiment 5
Detect reagent: H
+concentration be 100mmol/L, Hg
+concentration be 150mmol/L, Hg
2+concentration be 125mmol/L, NO
3 -concentration be 500mmol/L.2,4-dimethyl-5-aminoquinoline 50mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Embodiment 6
Detect reagent: H
+concentration be 2000mmol/L, Hg
+concentration be 200mmol/L, Hg
+concentration be 400mmol/L, NO
3 -concentration be 3000mmol/L.8-amino-2-methyl quinoline 100mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brown.
Embodiment 7
Detect reagent: H
+concentration be 2000mmol/L, Hg
+concentration be 500mmol/L, Hg
+concentration be 250mmol/L, NO
3 -concentration be 2800mmol/L, SO
4 2-concentration is 100mmol/L.2,4-dimethyl-5-aminopyridine 500mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Embodiment 8
Detect reagent: H
+concentration be 4000mmol/L, Hg
+concentration be 1200mmol/L, Hg
2+concentration be 600mmol/L, NO
3 -concentration be 6000mmol/L, 5-amino-4-methylpyrimidine 50mmol/L, all the other are sulfate ion, adopt aforesaid detection method, through overtesting find, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Embodiment 9
Detect reagent: H
+concentration be 4000mmol/L, Hg
+concentration be 1000mmol/L, Ni
2+concentration be 200mmol/L, NO
3 -concentration be 5000mmol/L, 4-acetylaminohydroxyphenylarsonic acid PA 40mmol/L, sodium molybdate 100mmol/L, all the other are sulfate ion, adopt aforesaid detection method, find through overtesting, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Embodiment 10
Detect reagent: H
+concentration be 4000mmol/L, Hg
+concentration be 1000mmol/L, Ni
2+concentration be 200mmol/L, NO
3 -concentration be 5000mmol/L, 9-amino-2-methoxyacridine 30mmol/L, all the other are sulfate ion, adopt aforesaid detection method, through overtesting find, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Embodiment 11
Detect reagent: H
+concentration be 4000mmol/L, Hg
+concentration be 1000mmol/L, Ni
2+concentration be 200mmol/L, NO
3 -concentration be 5000mmol/L, 2-amino-3-methyl pyrazine 30mmol/L, all the other are sulfate ion, adopt aforesaid detection method, through overtesting find, artificial urine 1 shows brick-red, and artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Embodiment 12
Detect reagent: H
+concentration be 4000mmol/L, Hg
+concentration be 1000mmol/L, Ni
2+concentration be 200mmol/L, NO
3 -concentration be 5000mmol/L, formyloxy-naphthalidine and 2, the potpourri 30mmol/L of 4-dimethyl-5-aminoquinoline (mol ratio 1:1), all the other are sulfate ion, adopt aforesaid detection method, find through overtesting, artificial urine 1 shows brick-red, artificial urine 2 whitening look, artificial urine 3 shows brick-red.
Comparative example 1
Detect reagent: H
+concentration be 2000mmol/L, Hg
+concentration be 100mmol/L, Ni
2+concentration be 400mmol/L, NO
3 -concentration be 2900mmol/L.Adopt aforesaid detection method, find through overtesting, artificial urine 1 shows brick-red, and artificial urine 2 shows brick-red, and artificial urine 3 shows brick-red.
Through the clinical verification of tumour hospital, 635 examples are in the cancer patient made a definite diagnosis through histology, and the accuracy rate (coincidence rate) of embodiment 1-7 reaches 98.1%, 95.0%, 94.0%, 98.1%, 94.0%, 94.6%, 92.8% respectively.Comparative example 1 is 85.8%.
The above, be only preferred embodiment of the present invention, be not intended to limit protection scope of the present invention.Every equalization done according to content of the present invention changes and modifies, and is all encompassed in the scope of the claims of the present invention.
Claims (10)
1. single hydroxyl phenolic metabolism thing urine detection reagent, described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl.
2. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, is characterized in that, described aromatic ring or the heteroaromatic compounds containing amino-compound with an amino group and an electron-donating group.
3. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, is characterized in that, described is containing amino aryl cyclic compound containing amino-compound or containing ammonia nitrogen heteroaromatic ring compounds, described amino group is positioned at the para postion of electron-donating group.
4. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, it is characterized in that, described aromatic ring is one or more in benzene, naphthalene, anthracene, phenanthrene, pyrene, aphthacene.
5. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, it is characterized in that, described fragrant heterocycle is pyridine, pyrimidine, pyrazine, pyridazine, triazine, quinoline, quinoxaline, acridine, imidazo [1,2-a] pyridine, imidazo [1,2-a] pyrimidine, pyrroles, furans, thiophene, isoquinoline, coumarone, imidazoles, carbazole, diazole, triazole.
6. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, is characterized in that, Hg in described detection reagent
+concentration be 50-1000mmol/L.
7. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, is characterized in that, H in described detection reagent
+concentration be 100-4000mmol/L.
8. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, is characterized in that, NO in described detection reagent
3 -concentration be 500-3000mmol/L.
9. single hydroxyl phenolic metabolism thing urine detection reagent according to claim 1, is characterized in that, the concentration containing amino-compound in described detection reagent is 0.01-500mmol/L.
10. the detection method of single hydroxyl phenolic metabolism thing, comprises the following steps,
-obtain human urine to be measured;
-reagent will be detected mix with human urine to be measured, obtain mixed liquor,
Described detection reagent is at least comprise nitric acid, mercurous nitrate and the aqueous solution containing amino-compound, and described have following structure containing amino-compound:
Ar is the aromatic ring of divalence or fragrant heterocycle, n is the integer of 1-6, and B is selected from one or more in alkoxy, ester group, amide group, phenyl, alkyl;
-judge the color of mixed liquor, when the color of mixed liquor be brick-red brown or rose time, then represent that human urine to be measured contains single hydroxyl phenolic metabolism thing, when the color of mixed liquor is white or faint yellow, then represent human urine to be measured not containing single hydroxyl phenolic metabolism thing.
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| CN201510539814.7A CN105136787A (en) | 2015-08-28 | 2015-08-28 | Detection reagent for mono-hydroxyphenyl metabolin urine |
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Cited By (3)
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| CN106645120A (en) * | 2016-11-21 | 2017-05-10 | 江苏华鸣生物科技有限公司 | Kit for detecting monohydroxyphenol metabolite in urine and preparation method of kit |
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| CN108287160A (en) * | 2017-01-10 | 2018-07-17 | 成都健浩生物科技有限公司 | The preparation method of the single hydroxyl amphyl measure reagent of urine |
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