CN105131048A - Purification and separation method of tea polyphenol from Pu'er tea residue - Google Patents
Purification and separation method of tea polyphenol from Pu'er tea residue Download PDFInfo
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- CN105131048A CN105131048A CN201510410910.1A CN201510410910A CN105131048A CN 105131048 A CN105131048 A CN 105131048A CN 201510410910 A CN201510410910 A CN 201510410910A CN 105131048 A CN105131048 A CN 105131048A
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Abstract
The invention relates to the technical field of beverages and particularly relates to a purification and separation method of tea polyphenol from Pu'er tea residue. The method includes following combined technologies: extraction, filtration, adsorption chromatography and the like. The method includes the steps of extracting a crude product of the tea polyphenol and then purifying the tea polyphenol crude product to prepare a pure product, wherein the content of the tea polyphenol in the pure product is more than 95%. The invention overcomes a problem of low production efficiency and purity of tea polyphenol in the background technology. The method is reduced in production cost of the tea polyphenol, is more environmental-protective and conforms to the spirit of sustainable development.
Description
Technical field
The present invention relates to natural product extractive technique field, be specifically related to tea-polyphenol method for purifying and separating in a kind of puer tea slag.
Background technology
Tea-polyphenol (TeaPolyphenols) is the general name of Polyphenols of Tea, comprises flavanol compound, anthocyanin class, flavonoid, flavonols and phenolic acids etc.Be mainly Flavonol (catechin) class, catechin accounts for 60% ~ 80%.Class material tea-polyphenol is tanned or tea tannin also known as tea, and being one of major ingredient forming Tea color fragrance, is also one of major ingredient having nourishing function in tealeaves.Research shows, tea-polyphenol contains multiple phenolic hydroxyl group therefore tool removing toxic substances and radiation resistance, radioactive substance effectively can be stoped to invade marrow, and strontium 90 and Co 60 can be made to excrete rapidly, be described as " radiation jinx " by healthy and medical circle.Tea-polyphenol is used widely in every field as a kind of additive, within 1989, is listed in GB2760-89 foodstuff additive by Food Additives Used in China association and uses standard, within 1997, be classified as Chinese patent medicine raw material.
Tea-polyphenol has very strong antitumor action.Tea-polyphenol (mainly catechin compounds) in tealeaves can block the multiple carcinogen in vivo synthesis such as nitrite, and there is effect of direct killing cancer cells and raising body immunity, to prevention and the assisting therapy of the kinds cancer such as cancer of the stomach, intestinal cancer, all helpful.
Leaf of Assam Tea, has another name called the blue or green tea in Yunnan, belongs to black tea, because former transportation and sales distribution centre is in Pu'er county, and therefore named Leaf of Assam Tea.Leaf of Assam Tea shines blue or green tea for raw material with big-leaf species in yunnan, uses the blue or green tea method for making of sub-fermentation, and from fermentation different points of make a living tea and ripe tea two kinds, finished product is divided into loose tea and compressed tea two class.Because Leaf of Assam Tea is in the courses of processing such as tea drink factory, tea comprehensive processing factory, create a large amount of tea grounds, the exhaust emission of these discarded tea grounds environment, does not meet the requirement of modern economy Sustainable development.In addition, along with the surge of world population, the shortage of grain and the energy is on the rise, and the exploitation of renewable plant resources have caused global common concern.
But in existing process for extracting tea polyphenol, often cost is higher, and have employed poisonous, the volatile organic solvent such as chloroform, production efficiency low-purity is lower, easily pollutes, unfriendly for environment.
Summary of the invention
The object of the present invention is to provide tea-polyphenol method for purifying and separating in a kind of puer tea slag, overcome puer tea slag in background technology and directly abandon waste resource, and the problem that tea-polyphenol production efficiency low-purity is lower, reduce the production cost of tea-polyphenol, and environmental protection more, meets the spirit of Sustainable development.
For realizing the object of the invention, the technical scheme of employing is:
Tea-polyphenol method for purifying and separating in a kind of puer tea slag, is characterized in that comprising the following steps:
Prepare tea-polyphenol crude extract:
A1. airing: puer tea slag is paved and carries out airing, airing thickness 1 ~ 3cm, airing 1 ~ 3 day, airing temperature controls at 20 ~ 24 degree;
A2. go out enzyme: tea grounds after airing is carried out boiling, boiling 15 ~ 20min, and temperature controls at 90 ~ 95 DEG C, suppresses and deactivation residual enzyme;
A3. slightly carry: the boiling water adding 8 ~ 10 times of tea grounds weight, extract 2 ~ 3 times, each 55 ~ 60min;
A4. ultrafiltration membrance filter: adopt ultra-filtration membrane to filter the solution after centrifugal, naturally cooling;
Prepare tea-polyphenol sterling:
B1. dissolve: tea-polyphenol crude extract is joined in sodium chloride solution, pH regulator to 7;
B2. centrifugal: centrifugal segregation particulate contamination;
B3. be separated: adopt polymeric amide to carry out adsorption chromatography as sorbent material, then carry out gradient elution with ethanol as eluent;
B4. concentrate drying: adopt thin-film evaporator to concentrate elutriant, after regulating pH to 7, is drying to obtain.
Preferably, the rotating speed during work of step B2 whizzer is 5000 ~ 5400r/min.
Preferably, during steps A 4 ultrafiltration membrance filter, the temperature of filtrate is 40 ± 2 DEG C.
By adopting above-mentioned technical scheme, the invention has the beneficial effects as follows: the present invention by the technology such as extraction, filtration, adsorption chromatography with the use of, in the crude product obtained after tea-polyphenol crude extract evaporate to dryness, polyphenol content is more than 70%, again through purification step, in sterling, polyphenol content is more than 95%, can save production cost in a large number, and do not use toxicity higher and volatile solvent, substantially increase the security of existence and product.Therefore, the present invention has complied with the tendency of the day of ecology, environmental protection, is industry exploitation safety, efficient, low cost tea-polyphenol method for extracting high purity, reduces one of active approaches of environmental pollution.
Embodiment
Be described in further detail below by specific embodiment.
Prepare tea-polyphenol crude extract:
A1. airing: puer tea slag is paved and carries out airing, airing thickness 1 ~ 3cm, airing 1 ~ 3 day, airing temperature controls at 20 ~ 24 degree;
A2. go out enzyme: tea grounds after airing is carried out boiling, boiling 15 ~ 20min, and temperature controls at 90 ~ 95 DEG C, suppresses and deactivation residual enzyme;
A3. slightly carry: the boiling water adding 8 ~ 10 times of tea grounds weight, extract 2 ~ 3 times, each 55 ~ 60min, filter, united extraction liquid, naturally cooling;
A4. ultrafiltration membrance filter: adopt ultra-filtration membrane to filter the solution after centrifugal, naturally cooling;
Prepare tea-polyphenol sterling:
B1. dissolve: tea-polyphenol crude extract is joined in sodium chloride solution, pH regulator to 7;
B2. centrifugal: centrifugal segregation particulate contamination;
B3. be separated: adopt polymeric amide to carry out adsorption chromatography as sorbent material, then carry out gradient elution with ethanol as eluent;
B4. concentrate drying: adopt thin-film evaporator to concentrate elutriant, after regulating pH to 7, is drying to obtain.
Preferably, the rotating speed during work of step B2 whizzer is 5000 ~ 5400r/min.
Preferably, during steps A 4 ultrafiltration membrance filter, the temperature of filtrate is 40 ± 2 DEG C.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. a tea-polyphenol method for purifying and separating in puer tea slag, is characterized in that comprising the following steps:
Prepare tea-polyphenol crude extract:
A1. airing: puer tea slag is paved and carries out airing, airing thickness 1 ~ 3cm, airing 1 ~ 3 day, airing temperature controls at 20 ~ 24 degree;
A2. go out enzyme: tea grounds after airing is carried out boiling, boiling 15 ~ 20min, and temperature controls at 90 ~ 95 DEG C, suppresses and deactivation residual enzyme;
A3. slightly carry: the boiling water adding 8 ~ 10 times of tea grounds weight, extract 2 ~ 3 times, each 55 ~ 60min, filter, united extraction liquid, naturally cooling;
A4. ultrafiltration membrance filter: adopt ultra-filtration membrane to filter the solution after centrifugal, naturally cooling;
Prepare tea-polyphenol sterling:
B1. dissolve: tea-polyphenol crude extract is joined in sodium chloride solution, pH regulator to 7;
B2. centrifugal: centrifugal segregation particulate contamination;
B3. be separated: adopt polymeric amide to carry out adsorption chromatography as sorbent material, then carry out gradient elution with ethanol as eluent;
B4. concentrate drying: adopt thin-film evaporator to concentrate elutriant, after regulating pH to 7, is drying to obtain.
2. tea-polyphenol method for purifying and separating in a kind of puer tea slag according to claim 1, is characterized in that: the rotating speed during work of step B2 whizzer is 5000 ~ 5400r/min.
3. tea-polyphenol method for purifying and separating in a kind of puer tea slag according to claim 1, is characterized in that: during steps A 4 ultrafiltration membrance filter, and the temperature of filtrate is 40 ± 2 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510410910.1A CN105131048A (en) | 2015-07-14 | 2015-07-14 | Purification and separation method of tea polyphenol from Pu'er tea residue |
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| CN201510410910.1A CN105131048A (en) | 2015-07-14 | 2015-07-14 | Purification and separation method of tea polyphenol from Pu'er tea residue |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113637090A (en) * | 2021-08-10 | 2021-11-12 | 张明勋 | Paper mulberry branch and bark material separation, decoction and extraction system and method |
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2015
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113637090A (en) * | 2021-08-10 | 2021-11-12 | 张明勋 | Paper mulberry branch and bark material separation, decoction and extraction system and method |
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| WD01 | Invention patent application deemed withdrawn after publication |