CN105110470B - A kind of dystopy activation method of the aging biomembrane of floating stuffing - Google Patents
A kind of dystopy activation method of the aging biomembrane of floating stuffing Download PDFInfo
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Abstract
The invention discloses a kind of dystopy activation method of the aging biomembrane of floating stuffing, belong to biomembrane technical field of sewage.Step of the invention is：First, the floating stuffing comprising aging biomembrane is taken out from stain disease treatment reactor；2nd, biomembrane volatile solid content is determined；3rd, processing scheme I or processing scheme II are selected according to volatile solid content numerical value respectively, aging biomembrane is processed；4th, washing removal filler surface loosened but without departing from biomembrane；5th, the floating stuffing containing a small amount of biomembrane is returned into throwing to reactor, completes dystopy activation.The present invention, using targetedly preferred scheme, effectively solves the aging biomembrane of floating stuffing and is difficult to nature and comes off update the problem of activation according to the fundamental characteristics of aging biomembrane, easy to operate and environment-friendly, with wide application prospect.
Description
Technical field
The present invention relates to biomembrance process technical field of sewage, more specifically to a kind of aging life of floating stuffing
The dystopy activation method of thing film.
Background technology
The biomembrane purification sewage that biomembrance process sewage disposal technology passes through filler superficial growth, adapts to water quantity and quality
Property removal effect strong, to organic matter the advantages of get well and have good nitrification and denitrification function, power cost and save, at stain disease
Extensive use is obtained in reason.Biomembrane comes off on filler surface after apposition growth, ripe and aging film, nascent membrane growth etc. is dynamic
State cyclic process, aerobic, simultaneous oxygen and anaerobic metabolism are carried out to pollutant.Filler in biofilm reactor is divided into fixed and outstanding
Floating two types, wherein, the moving bed/fluidized bed bio membrane reactor built using floating filler gathers conventional fluidization
Bed and two kinds of advantages of technique of biological contact oxidation process, operational management are convenient, are recent domestic biomembrance process sewage disposals
The focus of technology application.However, during moving bed/fluidized bed bio membrane reactor treatment actual waste water, over time, often
The aging biomembrane for filler surface often occur is difficult to nature and comes off the phenomenon of renewal, and biological membranous layer constantly thickeies to form " foundry loam ",
The ability and waste water treatment efficiency for being metabolized pollutant are greatly reduced, and influence the normal operation of processing system, it is necessary to activated again
It is raw.
Because floating stuffing density is smaller, easily collecting, the method for activating suitably using dystopy activation of its surface biological film, i.e.,
Floating stuffing is introduced into single device to be activated.Such as Chinese patent " the aging biofilm packing reparative regeneration side of one kind suspension
Method " (application number 201410734593.4), discloses a kind of aging suspended biological film filler reparative regeneration method, this application case bag
Include：1) the aging biomembrane suspended biological filler in biofilm reactor is taken out and is dried；2) Vibration on Start-up sieve, makes aging and pine
Dynamic biomembrane and the sludge of generation is gathered herein come off and thereafter let out；3) soda acid chemical removal；Using first mixing pickling, after
Alkali cleaning；4) enzyme preparation is further eluted；The enzyme preparation is protease；5) by step 4) filler after treatment dries, and will fill out
Material puts back to biofilm reactor；6) biomembrane regeneration；Air or oxygen are passed through, while it is right to add microelement nutritious liquid to realize
The final updated of biomembrane.This application case can remove the biomembrane of floating stuffing surface aging, fouling, effectively and quickly real
The renewal of existing biologic packing material is recycled, but it directly carries out a series of chemistry, biological and physical treatment, institute to aging biomembrane
Need relatively costly.
The content of the invention
1. the invention technical problem to be solved
The present invention is activated further to optimize the dystopy of carrier biofilm, there is provided a kind of aging biomembrane of floating stuffing
Dystopy activation method；The present invention optionally using preferred compositions scheme, and uses life according to the difference of aging Biofilm characteristics
Biodegradable, environmentally friendly biosurfactant, it is to avoid secondary pollution, can to greatest extent mitigate pollutant drop in biomembrane
The loss or inactivation of decorrelation microorganism, shorten the time for updating activation, while reducing activating cost.
2. technical scheme
To reach above-mentioned purpose, the technical scheme that the present invention is provided is：
A kind of dystopy activation method of the aging biomembrane of floating stuffing of the invention, its step is：
Step one, floating stuffing of the taking-up comprising aging biomembrane from stain disease treatment reactor；
Step 2, biomembrane volatile solid content is measured；
Step 3, the volatile solid content numerical value according to obtained by step 2 select different processing schemes respectively, to aging
Biomembrane is processed；
Step 4, washing removal filler surface loosened but without departing from biomembrane；
Step 5, the floating stuffing containing a small amount of biomembrane is returned throwing to reactor, complete dystopy activation.
Further, the stain disease treatment reactor in step one refers to be applied to municipal wastewater, industrial wastewater, micro- dirt
The biomembrance process processing unit of the stain disease treatment such as dye source water, its type includes aerobic and anoxic.
Further, floating stuffing is placed in receiving flask in step one, and adds absolute ethyl alcohol, ethanol in receiving flask
Mass percent concentration is 48%-52%.
Further, biomembrane volatile solid content continuous mode is as follows in step 2：
(1) floating stuffing is taken out from the receiving flask in step one, uses distilled water rinse, scraped using machinery or ultrasound is peeled off
Make biomembrane from filler sur-face peeling, biomembrane suspension, record suspension vol V is obtained；
(2) by step (1) gained suspension through prior drying, 0.45 μm of membrane filtration of constant weight；And will be with biomembrane
Filter membrane be placed in 105 DEG C of baking oven, drying is to constant weight；
(3) step (2) gained filter membrane is placed in and 0.5h is dried in drier, former filter membrane weight is subtracted after weighing, as shelled
The ratio between biomembrane amount of dry matter M1, amount of dry matter M1 and suspension vol V for falling are total solids content TS；
(4) step (2) gained filter membrane is placed in into calcination in 550 DEG C of Muffle furnace, to constant weight, to take out filter membrane and be placed in drier
In dry 0.5h after weigh, volatile biomembrane mass M 2, volatile biomembrane mass M 2 are through the weightlessness after calcination
Volatile solid content VS is with the ratio between suspension vol V；The ratio between volatile solid content VS and total solids content TS is
Biomembrane volatile solid content VS/TS.
Further, in step 3, when VS/TS >=0.7, using processing scheme I, i.e., it is with mass percent concentration
The rhamnolipid or the gained floating stuffing of APG solution steeping step one of 0.5%-2.5%, while carrying out ultrasonically treated 10-
25min；Work as VS/TS<When 0.7, using processing scheme II, i.e., the watery hydrochloric acid with mass percent concentration as 0.5%-1.5% is molten
The gained floating stuffing of liquid soaking step one, while carrying out ultrasonically treated 15-30min.
Further, ultrasound intensity is 25-35kHz in the processing scheme I and processing scheme II, floating stuffing and
The volume ratio of soaking solution is 0.4-1.0.
Further, step 4 uses pressure that filler is rinsed for the water of 0.2-0.3MPa, and removal filler surface has loosened
But without departing from biomembrane.
3. beneficial effect
The technical scheme provided using the present invention, compared with existing known technology, with following remarkable result：
(1) the dystopy activation method of the aging biomembrane of a kind of floating stuffing of the invention, according to aging biological film character
Difference, with biomembrane volatile solid content as criterion, distinguishes the aging life of class of organic matter accumulation type and inorganic salts appositional pattern etc. two
Thing film, using targetedly combining preferred scheme, the time is short, efficiency high, and eluent is reusable, low cost；
(2) a kind of dystopy activation method of the aging biomembrane of floating stuffing of the invention, aging for organic matter accumulation type
Biomembrane, using biodegradable, environmentally friendly biosurfactant, it is to avoid secondary pollution, and can mitigate to greatest extent
Inactivation of the processing procedure to contaminant degradation related microorganisms in biomembrane, shortens the time for updating activation；
(3) a kind of dystopy activation method of the aging biomembrane of floating stuffing of the invention, efficiently solves floating stuffing old
Change biomembrane be difficult to nature come off update activation problem, it is easy to operate and environment-friendly, with wide application prospect.
Brief description of the drawings
Fig. 1 is to carry out the operational flowchart that the aging biomembrane dystopy of floating stuffing is activated using the method for the present invention.
Specific embodiment
To further appreciate that present disclosure, the present invention is described in detail in conjunction with the accompanying drawings and embodiments.
Embodiment 1
A kind of dystopy activation method of the aging biomembrane of floating stuffing of the present embodiment, carries out certain aerobic moving bed biomembrane
The dystopy activation of the aging film of reactor floating stuffing.Floating stuffing is hollow cylinder, and inside is support support structure, external diameter and height
Degree is respectively 25mm and 10mm, and density is about 0.96g/cm3, effective ratio area is about 520m2/m3.The reactor influent quality
For：PH7.17~7.85,340~520mg/L of COD, 30~50mg/L of ammonia nitrogen, 60~90mg/L of total nitrogen, total phosphorus 3.2
~6.6mg/L, the μ S/cm of electrical conductivity 580~730.When operation was to the 86th day, there is " foundry loam " in filler surface, and waste water chemistry is needed
The clearance reduction by more than 15% of oxygen amount and ammonia nitrogen, biomembrane need to be activated.
The specific activation process of the present embodiment is：
Step one, some floating stuffings comprising aging biomembrane are taken out from reactor, be placed in receiving flask, added anhydrous
Ethanol so that ethanol mass percent concentration is 50% in receiving flask.
Step 2, biomembrane volatile solid content is measured；Receiving flask from step one takes out a certain amount of
Floating stuffing, with distilled water gently rinse, being scraped using machinery makes biomembrane from filler sur-face peeling, 2 parts of biomembranes is obtained and hangs
Supernatant liquid, records every part of suspension vol V.Above-mentioned suspension through drying in advance, 0.45 μm of membrane filtration (vacuum filtration) of constant weight,
Filter membrane with biomembrane is placed in 105 DEG C of baking oven, drying to constant weight (2h), takes out filter membrane and be placed in placement in drier
0.5h, subtracts former filter membrane weight after weighing, the biomembrane amount of dry matter M1 for as peeling off.Total solids content (TS) is dry
The ratio between amount M1 and suspension vol V, changes into mg/L.The filter membrane sample of above-mentioned another constant weight is placed in 550 DEG C of Muffle again
To constant weight (1h), taking-up filter membrane is placed in after placing 0.5h in drier and weighs, and being through the weightlessness after calcination to wave for calcination in stove
Hair property biomembrane mass M 2, the ratio between M2 and suspension vol V, as volatile solid content (VS) change into mg/L.Calculate
VS/TS, is 0.82 ± 0.068.
Step 3, due to volatile solid content (VS/TS) be more than 0.7, using processing scheme I, i.e. Bio-surface active
Agent and ultrasound are combined, specially：Soaked and ultrasonic (30kHz) with the rhamnolipid solution that mass percent concentration is 1% simultaneously
The volume ratio of floating stuffing 20min of the treatment comprising aging biomembrane, filler and soak is 0.6.
Step 4, use the filler that the water that pressure is 0.2MPa rinsed after being processed through step 3, washing removal filler surface
Loosened but without departing from biomembrane.By after this step process, aging biofilm detachment rate is up to 85% or so (weight in wet base)；
Step 5, the floating stuffing containing a small amount of biomembrane is returned throwing to reactor, complete dystopy activation：By floating stuffing
Repeat step 3, step 4 in batches, return throwing to reactor in the lump afterwards.After 2 days, chemical oxygen demand of waste water and ammonia nitrogen
Clearance improves 12% or so before relatively activating, and shows that biomembrane tentatively completes to update growth.
Inventor points out, traditional chemical treatment (acid, alkali, oxidant etc.) method, although cost is relatively low, effect is preferable,
But contaminant degradation related microorganisms in biomembrane can be produced inactivation, and be also easy to produce secondary pollution by its non-selectivity；At biology
Ferment treatment in reason optionally degradation characteristic composition (protein, polysaccharide etc.), the aging biomembrane of promotion can be peeled off, but by water quality
It is compared with easy in inactivation and relatively costly for influence (temperature, pH, toxicant etc.).
Biomembrane is mainly made up of microorganism and its extracellular polymeric, with multiphase it is heterogeneous the characteristics of, and due to waiting to locate
The difference of sewage quality is managed, the physicochemical property of biomembrane also has notable difference.Therefore, activated to aging biomembrane
When, targetedly processing scheme should be proposed on the basis of its fundamental characteristics is investigated.Aging biomembrane is by organic matter and inorganic matter
Composition, wherein, the main body-biofacies (bacterium, protozoan etc.) of degradation of contaminant is main to be existed in organic matter form.For old
Change the activation of biomembrane, biomembrane should be strengthened from the stripping on filler surface, while pollutant drops in mitigating biomembrane to greatest extent
The loss or inactivation of decorrelation microorganism, to shorten the follow-up time for updating activation.Based on this, the present embodiment is by aging biomembrane
It is divided into organic matter accumulation type and the major class of inorganic salts appositional pattern two, targetedly processing scheme is respectively adopted.Use for reference solids organic
The differentiation (VS/TS) of composition, by the research to biomembrane VS/TS, proposes that " VS/TS=0.7 " is the aging biology of two types
The separation of film, to protect in biomembrane beneficial organism phase and obtain actual activation effect.
The present embodiment is designed to use and targetedly combines preferred scheme according to the fundamental characteristics of biomembrane：For organic matter
The aging biomembrane of accumulation type, using biodegradable, environmentally friendly biosurfactant, by strengthening outside to extracellular microbial
The solubilization of polymer promotes biomembrane from filler sur-face peeling, and contaminant degradation correlation is micro- in mitigating biomembrane to greatest extent
Biological loss；For the aging biomembrane of inorganic salts appositional pattern, processed using dilute acid soln in short-term, strengthen the chemical solution of salt crust
Solution, while slowing down the inactivation to nearly filler side biofilm microorganisms.Efficiently solve the aging biomembrane of floating stuffing and be difficult to nature
Come off and update the problem of activation, it is easy to operate and environment-friendly, with wide application prospect.
Embodiment 2
A kind of dystopy activation method of the aging biomembrane of floating stuffing of the present embodiment, carries out certain anoxic fluidized bed bio film
The dystopy activation of the aging film of reactor floating stuffing.Floating stuffing is hollow cylinder, and inside is support support structure, external diameter and height
Degree is respectively 25mm and 10mm, and density is about 0.98g/cm3, effective ratio area is about 460m2/m3.The reactor influent quality
For：PH7.32~7.87,1500~2820mg/L of COD, 10~18mg/L of ammonia nitrogen, 54~86mg/L of total nitrogen, total phosphorus 13
~24mg/L, the μ S/cm of electrical conductivity 11864~16850.When operation was to the 68th day, the removal of chemical oxygen demand of waste water and ammonia nitrogen
Rate reduction by more than 20%, the appearance of filler surface biological film is aging, need to be activated.
The specific activation process of the present embodiment is：
Step one, some floating stuffings comprising aging biomembrane are taken out from reactor, be placed in receiving flask, added anhydrous
Ethanol so that concentration of alcohol is 52% in receiving flask.
Step 2, biomembrane volatile solid content is measured；Receiving flask from step one takes out a certain amount of
Floating stuffing, with distilled water gently rinse, being peeled off using ultrasonic (25kHz, 5min) makes biomembrane from filler sur-face peeling, is obtained
Biomembrane suspension, record suspension vol V.Above-mentioned suspension is through drying in advance, 0.45 μm of membrane filtration of constant weight (take out by vacuum
Filter), the filter membrane with biomembrane is placed in 105 DEG C of baking oven, drying to constant weight (2h), taking-up filter membrane is placed in drier to be put
.5h is set to 0, former filter membrane weight is subtracted after weighing, the biomembrane amount of dry matter M1 for as peeling off.Total solid (TS) is amount of dry matter
The ratio between M1 and suspension vol V, changes into mg/L.The filter membrane sample of above-mentioned constant weight is placed in calcination in 550 DEG C of Muffle furnace again
To constant weight (1h), taking-up filter membrane is placed in after placing 0.5h in drier and weighs, and volatile is through the weightlessness after calcination biological
The ratio between film quality M2, M2 and suspension vol V, as volatile solid content (VS), change into mg/L.VS/TS is calculated, is
0.68±0.054。
Step 3, be less than 0.7 due to volatile solid content (VS/TS), using processing scheme II, i.e., watery hydrochloric acid immersion and
Ultrasound combination, specially：Processed with dilute hydrochloric acid solution immersion and ultrasonic (35kHz) that mass percent concentration is 1.5% simultaneously
The volume ratio of the floating stuffing 20min comprising aging biomembrane, filler and soak is 0.5.
Step 4, use the filler that the water that pressure is 0.3MPa rinsed after being processed through step 3, washing removal filler surface
Loosened but without departing from biomembrane.By after this step process, aging biofilm detachment rate is up to 92% or so (weight in wet base)；
Step 5, the floating stuffing containing a small amount of biomembrane is returned throwing to reactor, complete dystopy activation：By floating stuffing
Repeat step 3, step 4 in batches, return throwing to reactor in the lump afterwards.After 6 days, chemical oxygen demand of waste water and ammonia nitrogen
Clearance is respectively increased 15% and 12% before relatively activating, and shows that biomembrane tentatively completes to update growth.
Embodiment 3
A kind of dystopy activation method of the aging biomembrane of floating stuffing of the present embodiment, substantially with embodiment 1, its difference
Place is：The step of the present embodiment three, is soaked and ultrasonic (35kHz) with the rhamnolipid solution that mass percent concentration is 0.5%
The volume ratio of floating stuffing 25min of the treatment comprising aging biomembrane, filler and soak is 0.4.
Embodiment 4
A kind of dystopy activation method of the aging biomembrane of floating stuffing of the present embodiment, substantially with embodiment 1, its difference
Place is：The step of the present embodiment three, is soaked and ultrasonic (25kHz) with the rhamnolipid solution that mass percent concentration is 2.5%
The volume ratio of floating stuffing 10min of the treatment comprising aging biomembrane, filler and soak is 1.0.
Embodiment 5
A kind of dystopy activation method of the aging biomembrane of floating stuffing of the present embodiment, substantially with embodiment 2, its difference
Place is：The step of the present embodiment, San Tong method was with the immersion of dilute hydrochloric acid solution that mass percent concentration is 0.5% and ultrasound
The volume ratio of floating stuffing 30min of (25kHz) treatment comprising aging biomembrane, filler and soak is 0.4.
Embodiment 6
A kind of dystopy activation method of the aging biomembrane of floating stuffing of the present embodiment, substantially with embodiment 2, its difference
Place is：The step of the present embodiment, San Tong method was with the immersion of dilute hydrochloric acid solution that mass percent concentration is 1.0% and ultrasound
The volume ratio of floating stuffing 15min of (30kHz) treatment comprising aging biomembrane, filler and soak is 1.0.
What deserves to be explained is, to those skilled in the art, under present inventive concept and specific embodiment enlightenment, energy
Enough some deformations directly derived from the disclosure of invention and general knowledge or associated, it will be recognized by those of ordinary skill in the art that
Also other method can be used, or commonly uses the replacement of known technology in the prior art, and mutually different combination between feature etc.
Unsubstantiality change, can equally be employed, can realize the present invention description function and effect, no longer one by one citing launch
Describe in detail, belong to the scope of the present invention.
Claims (3)
1. the dystopy activation method of the aging biomembrane of a kind of floating stuffing, its step is：
Step one, floating stuffing of the taking-up comprising aging biomembrane from stain disease treatment reactor；Receiving flask is placed in, and is added
Absolute ethyl alcohol, the mass percent concentration of ethanol is 48%-52% in receiving flask；
Step 2, biomembrane volatile solid content is measured；Continuous mode is as follows：
(1) floating stuffing is taken out from the receiving flask in step one, uses distilled water rinse, scraped using machinery or ultrasound is peeled off and makes life
Thing film is obtained biomembrane suspension, record suspension vol V from filler sur-face peeling；
(2) by step (1) gained suspension through prior drying, 0.45 μm of membrane filtration of constant weight；And the filter of biomembrane will be carried
Film is placed in 105 DEG C of baking oven, drying to constant weight；
(3) step (2) gained filter membrane is placed in and 0.5h is dried in drier, former filter membrane weight is subtracted after weighing, as peeled off
The ratio between biomembrane amount of dry matter M1, amount of dry matter M1 and suspension vol V are total solids content TS；
(4) step (2) gained filter membrane is placed in 550 DEG C of Muffle furnace calcination to constant weight, takes out filter membrane and be placed in drier and do
Weighed after dry 0.5h, be volatile biomembrane mass M 2 through the weightlessness after calcination, volatile biomembrane mass M 2 with it is outstanding
The ratio between supernatant liquid volume V is volatile solid content VS；The ratio between volatile solid content VS and total solids content TS is biology
Film volatile solid content VS/TS；
Step 3, the volatile solid content numerical value according to obtained by step 2 select different processing schemes respectively, to aging biology
Film is processed, specially：When VS/TS >=0.7, using processing scheme I, i.e., with mass percent concentration as 0.5%-
2.5% rhamnolipid or the gained floating stuffing of APG solution steeping step one, while carrying out ultrasonically treated 10-25min；
Work as VS/TS<When 0.7, using processing scheme II, i.e., the dilute hydrochloric acid solution immersion with mass percent concentration as 0.5%-1.5%
Step one gained floating stuffing, while carrying out ultrasonically treated 15-30min；
Step 4, washing removal filler surface loosened but without departing from biomembrane；
Step 5, the floating stuffing containing a small amount of biomembrane is returned throwing to reactor, complete dystopy activation.
2. the dystopy activation method of the aging biomembrane of a kind of floating stuffing according to claim 1, it is characterised in that：It is described
Ultrasound intensity is 25-35kHz in processing scheme I and processing scheme II, and the volume ratio of floating stuffing and soaking solution is
0.4-1.0。
3. the dystopy activation method of the aging biomembrane of a kind of floating stuffing according to claim 2, it is characterised in that：Step
Four use the water that pressure is 0.2-0.3MPa to rinse filler, removal filler surface loosened but without departing from biomembrane.
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