CN105105255A - Beverage and preparation method thereof - Google Patents
Beverage and preparation method thereof Download PDFInfo
- Publication number
- CN105105255A CN105105255A CN201510416411.3A CN201510416411A CN105105255A CN 105105255 A CN105105255 A CN 105105255A CN 201510416411 A CN201510416411 A CN 201510416411A CN 105105255 A CN105105255 A CN 105105255A
- Authority
- CN
- China
- Prior art keywords
- ferfas
- grams per
- per liter
- ribose
- zymotic fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013361 beverage Nutrition 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title abstract description 6
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims abstract description 70
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims abstract description 35
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims abstract description 35
- 238000000855 fermentation Methods 0.000 claims abstract description 34
- 230000004151 fermentation Effects 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 239000012530 fluid Substances 0.000 claims description 74
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 44
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 claims description 36
- 230000001580 bacterial effect Effects 0.000 claims description 32
- 238000012258 culturing Methods 0.000 claims description 32
- 239000001963 growth medium Substances 0.000 claims description 32
- 238000012216 screening Methods 0.000 claims description 32
- 229940041514 candida albicans extract Drugs 0.000 claims description 22
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 22
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 22
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 22
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 22
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 22
- 239000012138 yeast extract Substances 0.000 claims description 22
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 16
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 16
- 238000002955 isolation Methods 0.000 claims description 16
- 239000002609 medium Substances 0.000 claims description 16
- 238000000746 purification Methods 0.000 claims description 16
- 238000000926 separation method Methods 0.000 claims description 16
- 239000001888 Peptone Substances 0.000 claims description 14
- 108010080698 Peptones Proteins 0.000 claims description 14
- 235000019319 peptone Nutrition 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 11
- 229930006000 Sucrose Natural products 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 239000005720 sucrose Substances 0.000 claims description 11
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 229920002472 Starch Polymers 0.000 claims description 6
- 244000299461 Theobroma cacao Species 0.000 claims description 6
- 235000009470 Theobroma cacao Nutrition 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 4
- 238000005352 clarification Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 125000003603 D-ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 17
- 229940079593 drug Drugs 0.000 abstract description 15
- 241000609666 Tuber aestivum Species 0.000 abstract description 5
- 238000009776 industrial production Methods 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 14
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 230000009182 swimming Effects 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 208000033065 inborn errors of immunity Diseases 0.000 description 3
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 3
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000002929 anti-fatigue Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000000004 hemodynamic effect Effects 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 210000000955 splenic vein Anatomy 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- QGXBDMJGAMFCBF-HLUDHZFRSA-N 5α-Androsterone Chemical compound C1[C@H](O)CC[C@]2(C)[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CC[C@H]21 QGXBDMJGAMFCBF-HLUDHZFRSA-N 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- QGXBDMJGAMFCBF-UHFFFAOYSA-N Etiocholanolone Natural products C1C(O)CCC2(C)C3CCC(C)(C(CC4)=O)C4C3CCC21 QGXBDMJGAMFCBF-UHFFFAOYSA-N 0.000 description 1
- 206010015719 Exsanguination Diseases 0.000 description 1
- 102000015779 HDL Lipoproteins Human genes 0.000 description 1
- 108010010234 HDL Lipoproteins Proteins 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 208000007271 Substance Withdrawal Syndrome Diseases 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- BKTZCDBMEXUHDR-UHFFFAOYSA-N acetic acid azane Chemical compound N.N.N.CC(O)=O BKTZCDBMEXUHDR-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 229940061641 androsterone Drugs 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000009661 fatigue test Methods 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 150000003408 sphingolipids Chemical class 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of medicines, and provides a beverage and a preparation method thereof. The beverage is prepared from, by weight, 1-99 parts of a truffle fermentation liquid and 1-90 parts of D-ribose. The preparation method is simple, is easy to implement, and is suitable for industrial production.
Description
Technical field
The invention belongs to medical art, be specifically related to beverage containing ferfas, D-ribose and preparation method thereof.
Background technology
Ferfas (Truffle), also claims truffle, and have unique fragrance, mouthfeel and nutritive value, mankind's edible truffle has the history of more than one thousand years.Ferfas is rich in 17 seed amino acids, 8 kinds of vitamins, appropriate protein and androsterone, sterol, sphingolipid, aliphatic acid, amino acid and trace elements etc. more than 50 plant physiologically active ingredient.And containing the essential nutrients such as 8 seed amino acids, zinc, manganese, iron, calcium, phosphorus, selenium that human body self can not synthesize, there is develop immunitypty, anti-ageing, beneficial stomach, clear god, hemostasis, treat the medical values such as hemorrhoid, there is active anticancer, there is certain inhibitory action to cancer cell, can brain cell activity be excited.
The brewed health liquor fragrance of ferfas is unique, mouthfeel is good, green health and pharmacological action obvious, there is establishing-Yang, kidney tonifying, reduce serum low-density LP, cholesterol, increasing high density lipoprotein, atherosclerosis and the remarkable efficacy such as antitumor.
Summary of the invention
The object of the invention is to utilize fermentation technique to provide a kind of ferfas D ribose drink.
Specifically, the invention provides:
A kind of ferfas D-ribose beverage, described ferfas D-ribose beverage comprises: 1 ~ 99 weight portion ferfas zymotic fluid, 1 ~ 90 weight portion D-ribose.
Described ferfas D-ribose beverage comprises: 1 ~ 99 weight portion ferfas zymotic fluid, 1 ~ 90 weight portion D-ribose, 0.1 ~ 50 weight portion citrulling and/or cocoa power.
Described D-ribose is D-ribose powder or D-ribose concentrate.
Described ferfas zymotic fluid is prepared by following methods:
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 1 ~ 500 hour, zymotic fluid is obtained in 20 ~ 50 DEG C, 50 ~ 600 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 1 ~ 200 hour, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 20 ~ 50 DEG C, 50 ~ 600 revs/min cultivations; Described screening and culturing base is: glucose 10 ~ 100 grams per liter, peptone 1 ~ 20 grams per liter, yeast extract 1 ~ 20 grams per liter, magnesium sulfate 1 ~ 10 grams per liter, potassium dihydrogen phosphate 1 ~ 10 grams per liter, Oak Tree branches and leaves 10 ~ 100 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 1 ~ 500 hour in 20 ~ 50 DEG C, 50 ~ 600 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 0.1 ~ 300 hour, obtains ferfas symbiosis dominant strain zymotic fluid in 20 ~ 50 DEG C of cultivations; Symbiotic culture medium is: sucrose and/or maltose 1 ~ 100 grams per liter, yeast extract 1 ~ 20 grams per liter, wheat bran or Fructus Hordei Germinatus 1 ~ 100 grams per liter, potassium dihydrogen phosphate 1 ~ 10 grams per liter, magnesium sulfate 1 ~ 10 grams per liter, vitaminB10 .001 ~ 10 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 1 ~ 25% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 1 ~ 500 hour in 20 ~ 50 DEG C of cultivations; Described fermentation medium is ferfas symbiosis dominant strain zymotic fluid or composed of the following components: sucrose or glucose 1 ~ 100 grams per liter, yeast extract 1 ~ 50 grams per liter, peptone 1 ~ 50 grams per liter, malt extract 1 ~ 50 grams per liter, potassium dihydrogen phosphate 1 ~ 50 grams per liter, magnesium sulfate 1 ~ 50 grams per liter, soluble starch 1 ~ 200 grams per liter;
Step 4: ferfas aroma former zymotic fluid is processed 1-200 second under microwave power 10-600 watt, again according to 0.1 ~ 30% weight ratio add 1: 1 cellulase and pectase, 1 ~ 60 hour is incubated under temperature is 20 DEG C ~ 60 DEG C conditions, then heat to 70 DEG C ~ 125 DEG C, keep within 1 ~ 125 minute, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 1000 ~ 8000 revs/min, the centrifugal centrifugate obtained for 1 ~ 30 minute is ferfas zymotic fluid.
Described a kind of ferfas D-ribose beverage is prepared by following methods: mix after the clarification of ferfas zymotic fluid with D-ribose, sterilizing obtains ferfas D-ribose beverage.
Described a kind of ferfas D-ribose beverage is prepared by following methods: mix after the clarification of ferfas zymotic fluid with D-ribose, citrulling and/or cocoa power, sterilizing obtains beverage.
The present invention compared with prior art has the following advantages and good effect:
1, preparation method of the present invention is simple, is applicable to industrial production.
Detailed description of the invention
Below by way of the description of detailed description of the invention, the invention will be further described, but this is not limitation of the present invention, those skilled in the art are according to basic thought of the present invention, various amendment or improvement can be made, but only otherwise depart from basic thought of the present invention, all within the scope of the present invention.
Test example 1 improves immunity test
Experimental animal: BALB/C male mice, body weight 18-20g.
Trial drug:
Medicine 1 group: the product that in test example 1, comparative example obtains
Medicine 2 groups: the product that embodiment 9 obtains
Medicine 3 groups: the product that embodiment 10 obtains
Mouse modeling is hypoimmunity by test method: get mouse 20, random packet becomes 5 groups, and normal group not modeling, modeling successfully starts administration, and control group gives physiological saline 0.2ml/20g.Administration 1-3 group gastric infusion, dosage 30ml/kg, successive administration 30 days, 24 hours broken end sacrificed by exsanguination mouse after drug withdrawal, aseptically take out spleen, make splenocyte suspension with RPMI-1640 (containing 10% calf serum), mouse boosting cell is adjusted to 5 × 10
6cell/ml, is added in 96 well culture plates, every hole 100 μ l, and each hole adds double (namely adds ConA, and does not add ConA).ConA20 μ l (concentration is 1000 μ g/ml), RPMI-1640 80 μ l are every hole to 200 μ l volumes, in 5%CO
2cultivate 72 hours in 37 DEG C of insulating boxs, stop cultivating first 24 hours, every hole adds 3H-TdR3.7 × 10
9(iucr).With cell harvestor (TITERTEKCELLHARVESTER550) collecting cell in 49 type glass fiber filter paper, use distilled water, 5% triamine acetic acid, absolute ethanol washing successively, filter paper is placed in 60 DEG C of incubators to dry, be added in the cup containing 5ml scintillation solution, survey through liquid scintillation instrument (BECKMENLS9800) and participate in radioactive activity (cpm), then calculate SI (SI), namely SI=adds ConA hole cpm/ and does not add ConA hole cpm.
Table 1 different pharmaceutical is on the impact of hypoimmunity mice Splenic vein hemodynamics.
| Group | SI |
| Normal group | 0.689±0.071** |
| Control group | 0.125±0.125 |
| Medicine 1 group | 1.321±0.103** |
| Medicine 2 groups | 3.120±0.631**# |
| Medicine 3 groups | 3.096±0.583**# |
Compare with control group
*p < 0.01, compares #p < 0.05 with medicine 1 group
As shown in Table 2, the Truffle wine prepared by the present invention can make hypoimmunity mice Splenic vein hemodynamics significantly increase.
Test example 2 mouse anti-reflecting fatigue is tested
Experimental animal: Kunming mouse, body weight 18-20g.
Trial drug:
Medicine 1 group: the product that in test example 1, comparative example obtains
Medicine 2 groups: the product that embodiment 9 obtains
Medicine 3 groups: the product that embodiment 10 obtains
Test method: get mouse 20, random packet becomes 4 groups, and control group gives physiological saline 0.2ml/20g.Administration 1-3 group gastric infusion, dosage 30ml/kg, mouse, after 1 hour, is put into the swimming pool got ready in advance by administration fast, and record mouse enters the time that pond starts to no longer swimming.The results are shown in Table 2.
Table 2 anti-fatigue test result
| Group | Swimming time (min) |
| Control group | 2.5±0.8 |
| Medicine 1 group | 4.6±0.3** |
| Medicine 2 groups | 8.4±0.6**# |
| Medicine 3 groups | 8.6±0.8**# |
Compare with control group
*p < 0.01, compares #p < 0.05 with medicine 1 group
As shown in Table 2, the ferfas D ribose drink prepared by the present invention can the swimming time of significant prolongation mouse, illustrates that drink of the present invention has fine antifatigue effect.
Embodiment 1
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 300 hours, zymotic fluid is obtained in 30 DEG C, 260 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 160 hours, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 36 DEG C, 200 revs/min cultivations; Described screening and culturing base is: glucose 30 grams per liter, peptone 8 grams per liter, yeast extract 1.2 grams per liter, magnesium sulfate 1.1 grams per liter, potassium dihydrogen phosphate 1.3 grams per liter, Oak Tree branches and leaves 80 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 300 hours in 50 DEG C, 250 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 100 hours, obtains ferfas symbiosis dominant strain zymotic fluid in 30 DEG C of cultivations; Symbiotic culture medium is: maltose 70 grams per liter, yeast extract 16 grams per liter, wheat bran 80 grams per liter, potassium dihydrogen phosphate 2 grams per liter, magnesium sulfate 1.3 grams per liter, vitamin B12 .1 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 1 ~ 25% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 1 ~ 500 hour in 20 ~ 50 DEG C of cultivations; Described fermentation medium is composed of the following components: sucrose 80 grams per liter, yeast extract 30 grams per liter, peptone 20 grams per liter, malt extract 5 grams per liter, potassium dihydrogen phosphate 5 grams per liter, magnesium sulfate 3 grams per liter, soluble starch 100 grams per liter;
Step 4: ferfas aroma former zymotic fluid is processed 200 seconds under microwave power 10 watts, again according to 3% weight ratio add 1: 1 cellulase and pectase, under temperature is 20 DEG C of DEG C of conditions, is incubated 10 hours, then heats to 75 DEG C, keep within 25 minutes, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 6000 revs/min, the centrifugal centrifugate obtained for 20 minutes is ferfas zymotic fluid.
Embodiment 2
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 5 hours, zymotic fluid is obtained in 20 DEG C, 600 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 200 hours, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 20 DEG C, 600 revs/min cultivations; Described screening and culturing base is: glucose 100 grams per liter, peptone 1 grams per liter, yeast extract 1 grams per liter, magnesium sulfate 10 grams per liter, potassium dihydrogen phosphate 1 grams per liter, Oak Tree branches and leaves 100 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 500 hours in 50 DEG C, 50 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 3 hours, obtains ferfas symbiosis dominant strain zymotic fluid in 50 DEG C of cultivations; Symbiotic culture medium is: sucrose 100 grams per liter, yeast extract 1 grams per liter, wheat bran 100 grams per liter, potassium dihydrogen phosphate 1 grams per liter, magnesium sulfate 1 grams per liter, vitaminB10 .001 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 10% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 360 hours in 36 DEG C of cultivations; Described fermentation medium is composed of the following components: glucose 90 grams per liter, yeast extract 50 grams per liter, peptone 18 grams per liter, malt extract 20 grams per liter, potassium dihydrogen phosphate 9 grams per liter, magnesium sulfate 35 grams per liter, soluble starch 10 grams per liter;
Step 4: ferfas aroma former zymotic fluid is processed 180 seconds under microwave power 300 watts, again according to 2.5% weight ratio add 1: 1 cellulase and pectase, under temperature is 36 DEG C of conditions, is incubated 48 hours, then heats to 100 DEG C, keep within 95 minutes, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 7500 revs/min, the centrifugal centrifugate obtained for 20 minutes is ferfas zymotic fluid.
Embodiment 3
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 100 hours, zymotic fluid is obtained in 40 DEG C, 500 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 120 hours, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 28 DEG C, 400 revs/min cultivations; Described screening and culturing base is: glucose 80 grams per liter, peptone 1.8 grams per liter, yeast extract 17 grams per liter, magnesium sulfate 6.3 grams per liter, potassium dihydrogen phosphate 2.5 grams per liter, Oak Tree branches and leaves 80 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 200 hours in 30 DEG C, 400 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 80 hours, obtains ferfas symbiosis dominant strain zymotic fluid in 30 DEG C of cultivations; Symbiotic culture medium is: sucrose 80 grams per liter, yeast extract 12 grams per liter, Fructus Hordei Germinatus 40 grams per liter, potassium dihydrogen phosphate 3 grams per liter, magnesium sulfate 1.8 grams per liter, VB11 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 12% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 180 hours in 24 DEG C of cultivations; Described fermentation medium is ferfas symbiosis dominant strain zymotic fluid;
Step 4: ferfas aroma former zymotic fluid is processed 180 seconds under microwave power 400 watts, again according to 3% weight ratio add 1: 1 cellulase and pectase, under temperature is 40 DEG C of conditions, is incubated 16 hours, then heats to 95 DEG C, keep within 105 minutes, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 6000 revs/min, the centrifugal centrifugate obtained for 20 minutes is ferfas zymotic fluid.
Embodiment 4
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 200 hours, zymotic fluid is obtained in 45 DEG C, 100 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 160 hours, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 30 DEG C, 180 revs/min cultivations; Described screening and culturing base is: glucose 60 grams per liter, peptone 14 grams per liter, yeast extract 8 grams per liter, magnesium sulfate 3 grams per liter, potassium dihydrogen phosphate 9 grams per liter, Oak Tree branches and leaves 60 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 50 hours in 30 DEG C, 160 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 3 hours, obtains ferfas symbiosis dominant strain zymotic fluid in 30 DEG C of cultivations; Symbiotic culture medium is: sucrose 50 grams per liter, yeast extract 12 grams per liter, Fructus Hordei Germinatus 80 grams per liter, potassium dihydrogen phosphate 3 grams per liter, magnesium sulfate 6 grams per liter, vitaminB10 .08 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 1 ~ 25% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 1 ~ 500 hour in 20 ~ 50 DEG C of cultivations; Described fermentation medium is composed of the following components: sucrose 10 grams per liter, yeast extract 10 grams per liter, peptone 15 grams per liter, malt extract 5 grams per liter, potassium dihydrogen phosphate 50 grams per liter, magnesium sulfate 15 grams per liter, soluble starch 20 grams per liter;
Step 4: ferfas aroma former zymotic fluid is processed 120 seconds under microwave power 60 watts, again according to 3% weight ratio add 1: 1 cellulase and pectase, under temperature is 30 DEG C of conditions, is incubated 16 hours, then heats to 75 DEG C, keep within 25 minutes, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 3000 revs/min, the centrifugal centrifugate obtained for 10 minutes is ferfas zymotic fluid.
Embodiment 5
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 500 hours, zymotic fluid is obtained in 50 DEG C, 600 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 200 hours, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 50 DEG C, 600 revs/min cultivations; Described screening and culturing base is: glucose 100 grams per liter, peptone 20 grams per liter, yeast extract 20 grams per liter, magnesium sulfate 10 grams per liter, potassium dihydrogen phosphate 10 grams per liter, Oak Tree branches and leaves 100 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 500 hours in 50 DEG C, 600 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 300 hours, obtains ferfas symbiosis dominant strain zymotic fluid in 50 DEG C of cultivations; Symbiotic culture medium is: maltose 100 grams per liter, yeast extract 20 grams per liter, Fructus Hordei Germinatus 100 grams per liter, potassium dihydrogen phosphate 10 grams per liter, magnesium sulfate 10 grams per liter, VB11 0 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 25% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 500 hours in 20 ~ 50 DEG C of cultivations; Described fermentation medium is ferfas symbiosis dominant strain zymotic fluid;
Step 4: ferfas aroma former zymotic fluid is processed 200 seconds under microwave power 600 watts, again according to 30% weight ratio add 1: 1 cellulase and pectase, under temperature is 60 DEG C of conditions, is incubated 60 hours, then heats to 125 DEG C, keep within 125 minutes, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 8000 revs/min, the centrifugal centrifugate obtained for 30 minutes is ferfas zymotic fluid.
Embodiment 6
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 1 hour, zymotic fluid is obtained in 20 DEG C, 50 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 1 hour, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 20 DEG C, 50 revs/min cultivations; Described screening and culturing base is: glucose 10 grams per liter, peptone 1 grams per liter, yeast extract 1 grams per liter, magnesium sulfate 1 grams per liter, potassium dihydrogen phosphate 1 grams per liter, Oak Tree branches and leaves 10 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 1 hour in 20 DEG C, 50 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 0.1 hour, obtains ferfas symbiosis dominant strain zymotic fluid in 20 DEG C of cultivations; Symbiotic culture medium is: sucrose 1 grams per liter, yeast extract 1 grams per liter, wheat bran 1 grams per liter, potassium dihydrogen phosphate 1 grams per liter, magnesium sulfate 1 grams per liter, vitaminB10 .001 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 1% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 1 hour in 20 DEG C of cultivations; Described fermentation medium is composed of the following components: sucrose 1 grams per liter, yeast extract 1 grams per liter, peptone 1 grams per liter, malt extract 1 grams per liter, potassium dihydrogen phosphate 1 grams per liter, magnesium sulfate 1 grams per liter, soluble starch 1 grams per liter;
Step 4: ferfas aroma former zymotic fluid is processed 1 second under microwave power 10 watts, again according to 0.1% weight ratio add 1: 1 cellulase and pectase, under temperature is 20 DEG C of conditions, is incubated 1 hour, then heats to 70 DEG C DEG C, keep within 1 minute, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 1000 revs/min, the centrifugal centrifugate obtained for 1 minute is ferfas zymotic fluid.
Embodiment 7
80g ferfas zymotic fluid obtained for embodiment 6,24gD-ribose, 6g citrulling, 1.5g cocoa power are joined in the distilled water of 1000ml, mixes rear filtration, sterilizing, obtain ferfas D-ribose drink.
Embodiment 8
30g ferfas zymotic fluid obtained for embodiment 3,15gD-ribose, 3g citrulling are joined in the distilled water of 1000ml, mixes rear filtration, sterilizing, obtain ferfas D-ribose drink.
Embodiment 9
10g ferfas zymotic fluid obtained for embodiment 5,10gD-ribose, 7g cocoa power are joined in the distilled water of 1000ml, mixes rear filtration, sterilizing, obtain ferfas D-ribose drink.
Embodiment 10
10g ferfas zymotic fluid obtained for embodiment 1,10gD-ribose are joined in the distilled water of 1000ml, mixes rear filtration, sterilizing, obtain ferfas D-ribose drink.
Claims (6)
1. a ferfas D-ribose beverage, is characterized in that, described ferfas D-ribose beverage comprises: 1 ~ 99 weight portion ferfas zymotic fluid, 1 ~ 90 weight portion D-ribose.
2. a kind of ferfas D-ribose beverage according to claim 1, is characterized in that, described ferfas D-ribose beverage comprises: 1 ~ 99 weight portion ferfas zymotic fluid, 1 ~ 90 weight portion D-ribose, 0.1 ~ 50 weight portion citrulling and/or cocoa power.
3. a kind of ferfas D-ribose beverage according to claim 1,2, is characterized in that, described D-ribose is D-ribose powder or D-ribose concentrate.
4. a kind of ferfas D-ribose beverage according to claim 1, it is characterized in that, described ferfas zymotic fluid is prepared by following methods:
Step 1: the separation and purification in MS improved culture medium of ferfas ascocarp tissue isolation is obtained a collection of pure bacterial strain, it is accessed respectively in screening and culturing base, within 1 ~ 500 hour, zymotic fluid is obtained in 20 ~ 50 DEG C, 50 ~ 600 revs/min cultivations, the bacterial strain that in zymotic fluid, alpha-androstanol content is high had both been ferfas aroma former, within 1 ~ 200 hour, obtained ferfas aroma former seed liquor by ferfas aroma former access screening and culturing base in 20 ~ 50 DEG C, 50 ~ 600 revs/min cultivations; Described screening and culturing base is: glucose 10 ~ 100 grams per liter, peptone 1 ~ 20 grams per liter, yeast extract 1 ~ 20 grams per liter, magnesium sulfate 1 ~ 10 grams per liter, potassium dihydrogen phosphate 1 ~ 10 grams per liter, Oak Tree branches and leaves 10 ~ 100 grams per liter;
Step 2: the separation and purification in MS improved culture medium of ferfas symbiosis mycorrhiza tissue isolation is obtained a collection of pure bacterial strain, it is inoculated in screening and culturing base respectively together with ferfas aroma former, cultivate 1 ~ 500 hour in 20 ~ 50 DEG C, 50 ~ 600 revs/min, the bacterial strain that in zymotic fluid, mycelium content is high is ferfas symbiosis dominant strain, is linked into by ferfas symbiosis dominant strain in symbiotic culture medium and within 0.1 ~ 300 hour, obtains ferfas symbiosis dominant strain zymotic fluid in 20 ~ 50 DEG C of cultivations; Symbiotic culture medium is: sucrose and/or maltose 1 ~ 100 grams per liter, yeast extract 1 ~ 20 grams per liter, wheat bran or Fructus Hordei Germinatus 1 ~ 100 grams per liter, potassium dihydrogen phosphate 1 ~ 10 grams per liter, magnesium sulfate 1 ~ 10 grams per liter, vitaminB10 .001 ~ 10 grams per liter;
Step 3: by ferfas aroma former seed liquor according in 1 ~ 25% weight ratio access fermentation medium, obtain ferfas aroma former zymotic fluid for 1 ~ 500 hour in 20 ~ 50 DEG C of cultivations; Described fermentation medium is ferfas symbiosis dominant strain zymotic fluid or composed of the following components: sucrose or glucose 1 ~ 100 grams per liter, yeast extract 1 ~ 50 grams per liter, peptone 1 ~ 50 grams per liter, malt extract 1 ~ 50 grams per liter, potassium dihydrogen phosphate 1 ~ 50 grams per liter, magnesium sulfate 1 ~ 50 grams per liter, soluble starch 1 ~ 200 grams per liter;
Step 4: ferfas aroma former zymotic fluid is processed 1-200 second under microwave power 10-600 watt, again according to 0.1 ~ 30% weight ratio add 1: 1 cellulase and pectase, 1 ~ 60 hour is incubated under temperature is 20 DEG C ~ 60 DEG C conditions, then heat to 70 DEG C ~ 125 DEG C, keep within 1 ~ 125 minute, obtaining ferfas enzymatic hydrolysis and fermentation liquid;
Step 5: by ferfas enzymatic hydrolysis and fermentation liquid at 1000 ~ 8000 revs/min, the centrifugal centrifugate obtained for 1 ~ 30 minute is ferfas zymotic fluid.
5. a kind of ferfas D-ribose beverage according to claim 1, is characterized in that being prepared by following methods: mix after the clarification of ferfas zymotic fluid with D-ribose, sterilizing obtains ferfas D-ribose beverage.
6. a kind of ferfas D-ribose beverage according to claim 2, is characterized in that being prepared by following methods: mix after the clarification of ferfas zymotic fluid with D-ribose, citrulling and/or cocoa power, sterilizing obtains beverage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510416411.3A CN105105255A (en) | 2015-07-16 | 2015-07-16 | Beverage and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510416411.3A CN105105255A (en) | 2015-07-16 | 2015-07-16 | Beverage and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105105255A true CN105105255A (en) | 2015-12-02 |
Family
ID=54653581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510416411.3A Pending CN105105255A (en) | 2015-07-16 | 2015-07-16 | Beverage and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105105255A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105661491B (en) * | 2015-12-30 | 2018-10-30 | 仲恺农业工程学院 | A kind of peach gum health products and its preparation method and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010063021A1 (en) * | 2008-11-28 | 2010-06-03 | Archer Daniels Midland Company | Method of washing cocoa beans to improve the quality of the cocoa products obtained from such beans |
| CN102145019A (en) * | 2011-03-29 | 2011-08-10 | 郭景龙 | Medicinal composition containing truffles, vitamins and mineral substances |
| CN102948903A (en) * | 2012-11-30 | 2013-03-06 | 郭景龙 | Health-care beverage |
| CN103783226A (en) * | 2014-02-19 | 2014-05-14 | 郭景龙 | Soy sauce flavor type tea |
| CN103859122A (en) * | 2014-04-02 | 2014-06-18 | 郭景龙 | Maotai flavored coffee and preparation method thereof |
-
2015
- 2015-07-16 CN CN201510416411.3A patent/CN105105255A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010063021A1 (en) * | 2008-11-28 | 2010-06-03 | Archer Daniels Midland Company | Method of washing cocoa beans to improve the quality of the cocoa products obtained from such beans |
| CN102145019A (en) * | 2011-03-29 | 2011-08-10 | 郭景龙 | Medicinal composition containing truffles, vitamins and mineral substances |
| CN102948903A (en) * | 2012-11-30 | 2013-03-06 | 郭景龙 | Health-care beverage |
| CN103783226A (en) * | 2014-02-19 | 2014-05-14 | 郭景龙 | Soy sauce flavor type tea |
| CN103859122A (en) * | 2014-04-02 | 2014-06-18 | 郭景龙 | Maotai flavored coffee and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105661491B (en) * | 2015-12-30 | 2018-10-30 | 仲恺农业工程学院 | A kind of peach gum health products and its preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102038046B (en) | Production method of Pu'er tea ferment liquid, ferment powder and ferment beverage | |
| CN104432388B (en) | Shiny-leaved yellowhorn peptide nutrient food and preparation method thereof | |
| Reshetnikov et al. | Medicinal value of the genus Tremella Pers.(Heterobasidiomycetes) | |
| CN112999261B (en) | Natto fermented composition capable of relieving arteriosclerosis and preparation method and application thereof | |
| CN102524396B (en) | A kind of agitating type Pleurotus eryngii nutritional yogurt drink and preparation method thereof | |
| CN108244432A (en) | A kind of fermentation Cordyceps militaris probiotic beverage and preparation method thereof | |
| CN104382164A (en) | Beverage prepared from lucid ganoderma and black tea fungus | |
| CN102224873B (en) | Method for preparing food from raw materials comprising coffee beans | |
| CN105559068B (en) | It is a kind of to utilize composition for eating the acquisition of medicine fungi fermentation radix tetrastigme and preparation method thereof | |
| CN103301321B (en) | Thrombolytic active polysaccharide mixture preparation technology | |
| CN104432341B (en) | The preparation method of Fresh Cucumber Juice fermented beverage | |
| CN104893950A (en) | Method for preparing cordyceps sinensis fermented vinegar beverage | |
| CN102816667B (en) | Ganoderma lucidum polysaccharide nutrition sweet wine and production process | |
| CN106262837A (en) | A kind of Fructus Musae Fructus Lycii ferment and preparation method thereof | |
| CN102224925B (en) | Method for preparing food from truffle and coffee beans as raw materials | |
| CN105907831A (en) | Preparation method of high-purity agaricus blazei murrill polysaccharide | |
| CN105105255A (en) | Beverage and preparation method thereof | |
| CN105288226A (en) | Kidney-invigorating and yang-strengthening composition and preparation method thereof | |
| CN111700142A (en) | Preparation method of fermented pachymic acid substituted tea | |
| CN1273783A (en) | Process for preparing active peptide of soybean protein | |
| CN103525684B (en) | Deep glossy ganoderma fermenting liquid prepares the method for Ganoderma vinegar | |
| CN105535035B (en) | A kind of Inonotus obliquus fermented and cultured composition and preparation method thereof | |
| CN113558126A (en) | Method for producing nutritious coffee by using ganoderma lucidum mycelium | |
| CN108402364A (en) | A kind of truffle drink | |
| CN102166029A (en) | Functional drink of multi-bacterium health-keeping extract of mesona chinensis benth |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151202 |
|
| WD01 | Invention patent application deemed withdrawn after publication |