CN105087757A - Molecular marker for identifying hundred-grain weight of soybeans and application of molecular marker - Google Patents
Molecular marker for identifying hundred-grain weight of soybeans and application of molecular marker Download PDFInfo
- Publication number
- CN105087757A CN105087757A CN201410186304.1A CN201410186304A CN105087757A CN 105087757 A CN105087757 A CN 105087757A CN 201410186304 A CN201410186304 A CN 201410186304A CN 105087757 A CN105087757 A CN 105087757A
- Authority
- CN
- China
- Prior art keywords
- soybean
- grain weight
- sequence
- dna
- dna fragmentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a molecular marker for identifying hundred-grain weight of soybeans and application of the molecular marker. Compared with an actual measurement result on hundred-grain weight of the soybeans, a measurement result of the molecular marker has characteristics that primer Bar csoyssr_13_890 consists of single-stranded DNA (deoxyribonucleic acid) shown in a sequence 1 of a sequence table and single-stranded DNA shown in a sequence 2 of the sequence table, genome DNA of to-be-measured soybeans which comprise 126 strains of a F8-generation heterozygous system group of which female parent is Jidou 12 and male parent is wild soybeans ZYD2738 is subjected to PCR (polymerase chain reaction) amplification, the hundred-grain weight of the to-be-measured soybeans is predicted by the obtained molecular marker, and accuracy can reach 94.44%. The molecular marker can be used for identifying or screening hundred-grain weight of soybean varieties or strains, breeding efficiency is greatly improved, and application prospect in actual breeding and production is wide.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of molecule marker and the application thereof of identifying soybean 100-grain weight height.
Background technology
Soybean (Glycinemax) is the important crops integrating grain and oil, feed and industrial use, for the mankind provide the food plant protein of 65% and the edible oil of 31%.100-grain weight is the important yield component of soybean, has larger direct effect to output.Due to the quantitative character that 100-grain weight is complicated, be subject to genetic background and environmental influence, it is significant that the height of Accurate Prediction soybean 100-grain weight carries out soybean breeder to screening high yielding soybeans kind.
Molecule marker is the genetic marker by between individuality based on genetic material inner nucleotide sequence variations, is the direct reflection of DNA level genetic polymorphism.With other several genetic markers---morphology marks, compared with biochemical biomarker, cytological marker, the superiority that DNA molecular marker has has: most of molecule marker is codominance, very convenient to the selection of the proterties of recessiveness; Genome mutation is extremely abundant, and the quantity of molecule marker is almost unlimited; In the different steps of biological development, the DNA of different tissues can be used for labeled analysis; Molecule marker discloses the variation from DNA; Show as neutrality, do not affect the expression of objective trait, with bad proterties without chain; Detection means is simple, rapid.
Summary of the invention
An object of the present invention is to provide a kind of method of qualification or assistant identification soybean 100-grain weight height.
Method provided by the invention, comprise the genomic dna with Barcsoyssr_13_890 primer pair pcr amplification soybean to be measured, detect PCR primer, if the product of described pcr amplification is DNA fragmentation A and DNA fragmentation B, then described soybean to be measured is soybean that 100-grain weight is high or candidate is the soybean that 100-grain weight is high; If the product of described pcr amplification is described DNA fragmentation A or B, then described soybean to be measured is soybean that 100-grain weight is low or candidate is the soybean that 100-grain weight is low;
The primer pair that described Barcsoyssr_13_890 primer pair is made up of the single stranded DNA shown in the single stranded DNA shown in sequence 1 and sequence 2,
The PCR primer that the genomic dna that described DNA fragmentation A is No. 12, the primer pair pcr amplification the cultivated soybean kind Ji beans with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains;
The PCR primer that the genomic dna that described DNA fragmentation B is the primer pair pcr amplification wild soybean ZYD2738 with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains.
In aforesaid method, the method detecting PCR primer is electrophoresis or order-checking.
In an embodiment of the present invention, if PCR primer is DNA fragmentation A and DNA fragmentation B, then this PCR primer is that heterozygosis banding pattern (hybridize and multiply to F by No. 12, Ji beans and wild soybean ZYD2738
8the banding pattern of the generation in generation); If PCR primer is DNA fragmentation A or DNA fragmentation B, then this PCR primer is parent's banding pattern (banding pattern of No. 12, Ji beans or wild soybean ZYD2738); The size of DNA fragmentation A is 120-125bp, is specially 125bp, and the size of DNA fragmentation B is 135-140bp, is specially 135bp.
In aforesaid method, described 100-grain weight is high refers to that the 100-grain weight of described soybean to be measured is 10-13g;
Described 100-grain weight is low refers to that the 100-grain weight of described soybean to be measured is 5-8g.
In an embodiment of the present invention, the 100-grain weight that described 100-grain weight is specially water content after soybean kernel maturation to be measured when being 12%.
In aforesaid method, described soybean to be measured is No. 12, soybean varieties Ji beans, wild soybean ZYD2738 or by the derivative kind of No. 12, described Ji beans and wild soybean ZYD2738 or strain.
In aforesaid method, the described kind derivative with wild soybean ZYD2738 by No. 12, Ji beans or product are that No. 12, described Ji beans and wild soybean ZYD2738 are hybridized and multiplied to F
2for the above generation.In an embodiment of the present invention, F is specially
8generation.
Another object of the present invention be to provide a kind of for the identification of or assistant identification soybean 100-grain weight height DNA fragmentation.
DNA fragmentation provided by the invention following 1) or 2) DNA fragmentation:
1) DNA fragmentation A and DNA fragmentation B;
2) DNA fragmentation A or DNA fragmentation B;
The PCR primer that the genomic dna that described DNA fragmentation A is No. 12, the primer pair pcr amplification the cultivated soybean kind Ji beans with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains;
The PCR primer that the genomic dna that described DNA fragmentation B is the primer pair pcr amplification wild soybean ZYD2738 with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains;
Described soybean to be measured is No. 12, soybean varieties Ji beans, wild soybean ZYD2738 or by the derivative kind of No. 12, described Ji beans and wild soybean ZYD2738 or strain.
In above-mentioned DNA fragmentation, described 100-grain weight is high refers to that the 100-grain weight of described soybean to be measured is 10-13g;
Described 100-grain weight is low refers to that the 100-grain weight of described soybean to be measured is 5-8g;
The product of described pcr amplification electrophoretic separation in the polyacrylamide gel of 5-7% obtains by described DNA fragmentation A and described DNA fragmentation B.
Barcsoyssr_13_890 primer pair or aforesaid method or the application of above-mentioned DNA fragmentation in qualification or assistant identification soybean 100-grain weight height are also the scope of protection of the invention;
Or Barcsoyssr_13_890 primer pair or aforesaid method or above-mentioned DNA fragmentation are also being the scope of protection of the invention for the preparation of the application in qualification or assistant identification soybean 100-grain weight height low production.
Barcsoyssr_13_890 primer pair or aforesaid method or the application of above-mentioned DNA fragmentation in the soybean that the described 100-grain weight of screening is high are also the scope of protection of the invention.
Barcsoyssr_13_890 primer pair or aforesaid method or the application of the DNA fragmentation stated in the soybean that the described 100-grain weight of screening is low is also the scope of protection of the invention.
Barcsoyssr_13_890 primer pair or state method or the application of the DNA fragmentation stated in soybean breeder is also the scope of protection of the invention.
3rd object of the present invention is to provide and a kind ofly obtains the high soybean method of 100-grain weight.
Method provided by the invention, comprises the steps:
1), No. 12, Ji beans and wild soybean ZYD2738 hybridize, and obtains hybrid generation, then selfing, obtains self-bred progeny;
2), with the genomic dna of Barcsoyssr_13_890 primer pair pcr amplification self-bred progeny soybean, detect PCR primer, if the product of described pcr amplification is DNA fragmentation A and DNA fragmentation B, then described soybean to be measured is soybean that 100-grain weight is high or candidate is the soybean that 100-grain weight is high;
The primer pair that described Barcsoyssr_13_890 primer pair is made up of the single stranded DNA shown in the single stranded DNA shown in sequence 1 and sequence 2,
The PCR primer that the genomic dna that described DNA fragmentation A is No. 12, the primer pair pcr amplification the cultivated soybean kind Ji beans with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains;
The PCR primer that the genomic dna that described DNA fragmentation B is the primer pair pcr amplification wild soybean ZYD2738 with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains.
In aforesaid method,
Described 100-grain weight is high refers to that the 100-grain weight of described soybean to be measured is 10-13g;
Described self-bred progeny is F
2for the above generation, in an embodiment of the present invention, F is specially
8generation.
Experiment of the present invention proves, compared with the result of actual measurement soybean 100-grain weight, with the primer pair Barcsoyssr_13_890 of the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2, pcr amplification soybean to be measured (with No. 12, Ji beans be female parent, the wild soybean ZYD2738 F that is male parent
8126 strains for colony of heterozygosis system) genomic dna, by the above-mentioned soybean 100-grain weight height to be measured of the DNA fragmentation that obtains prediction, accuracy rate can reach 94.44%.The present invention can be used for the 100-grain weight height identifying or screen soybean varieties or strain, greatly improves breeding efficiency, has broad application prospects in actual breed and production.
Accompanying drawing explanation
Fig. 1 is the 6% polyacrylamide gel electrophoresis figure obtaining PCR primer with primer pair pcr amplification soybean to be measured.
Embodiment
The experimental technique used in following embodiment if no special instructions, is ordinary method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
No. 12, the cultivated soybean kind Ji beans (Glycinemax (L.) Merr) used in following embodiment are purchased from Hebei Ji Feng agricultural-food Science and Technology Ltd..
Used in following embodiment wild soybean ZYD2738 (GlycinesojaSieb.EtZucc.): Yan Long. Soybean Interspecific Crosses (Glycinemax × G.soja) offspring grain characters QTL locates [D]. Beijing. the Chinese Academy of Agricultural Sciences, 2011, the public can obtain from Food and Oil Crops Inst., Hebei Agriculture and Forestry Academy.
Embodiment 1, molecule marker is utilized to measure No. 12, soybean varieties Ji beans and wild soybean ZYD2738
1, primer pair
Design primer pair, is made up of the single strand dna shown in sequence 2 in the single strand dna shown in sequence in sequence table 1 and sequence table.
2, molecule marker is utilized to measure No. 12, soybean varieties Ji beans and wild soybean ZYD2738
SDS method is utilized to extract the genomic dna of No. 12, soybean varieties Ji beans, with this genomic dna for template, pcr amplification is carried out with the primer Barcsoyssr_13_890 of the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2, get 10 μ lPCR amplified productions, at 95 DEG C of sex change 3min, put immediately to cooled on ice, by 6% polyacrylamide gel (sequencing gel) electrophoretic separation 1.5 hours under 90W invariable power, through Marker comparison, obtaining size is the DNA fragmentation A being about 125bp.
SDS method is utilized to extract the genomic dna of wild soybean ZYD2738, with this genomic dna for template, pcr amplification is carried out with the primer Barcsoyssr_13_890 of the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2, get 10 μ lPCR amplified productions, at 95 DEG C of sex change 3min, put immediately to cooled on ice, by 6% polyacrylamide gel (sequencing gel) electrophoretic separation 1.5 hours under 90W invariable power, through Marker comparison, obtain the DNA fragmentation B that size is about 135bp.
Reaction system and the condition of above-mentioned pcr amplification are as follows:
Reaction system (20 μ l): 4 μ l template DNAs (30ng/ μ l), the each 0.3 μ l of upstream and downstream primer (final concentration of every bar primer in reaction system is 10 μMs), 1.5 μ ldNTPs (2.5mM), 2.0 μ l10 × PCRBuffer are (containing 15mMMg
2+), 0.2 μ lTaq enzyme (5U/ μ l) and 11.7 μ lddH
2o.
Reaction conditions: 94 DEG C of denaturation 5min, the cycle stage: 94 DEG C of sex change 30s; 47 DEG C of annealing 30s; 72 DEG C extend 30s, circulate 35 times, and last 72 DEG C extend 5min, and PCR primer is in 4 DEG C of preservations.
Above-mentioned 6% polyacrylamide gel (sequencing gel): 57 grams of acrylamides (Acrylamide), 3 grams of Bbs (Bis), 420 grams of urea (Urea), (solvent is water to 100 milliliters of 10 × tbe buffer liquid, solute and concentration thereof are tris108g/L, boric acid 55g/L, EDTA7.43g/L), add ddH
2o to 1000 milliliter.
Embodiment 2, molecular markers for identification or No. 12, assistant identification soybean varieties Ji beans and wild soybean ZYD2738 is utilized to derive the 100-grain weight of strain
Utilize the molecule marker Barcsoyssr_13_890 of embodiment 1 to carry out 100-grain weight prediction to soybean to be measured, factual survey carried out to soybean 100-grain weight to be measured simultaneously, concrete grammar and result as follows:
1, soybean to be measured: be maternal, wild soybean ZYD2738 with Ji beans 12 be paternal hybrid, F after selfing
8for colony of heterozygosis system, totally 126 familys;
Check variety: soybean varieties Ji beans 12 and wild soybean ZYD2738.
2, the prediction of Markers for Detection and 100-grain weight height
Extract F respectively
8126 family soybean leaves in generation extract genomic dna by SDS method, pcr amplification is carried out with the primer pair Barcsoyssr_13_890 of embodiment 1, respectively get 10 μ lPCR amplified productions, at 95 DEG C of sex change 3min, put immediately to cooled on ice, electrophoretic separation about 1.5 hours under 90W invariable power on 6% polyacrylamide sequencing gel, takes pictures after silver dye, development.
Reaction system (20 μ l): 4 μ l template DNAs (30ng/ μ l), the each 0.3 μ l of upstream and downstream primer (final concentration of every bar primer in reaction system is 10 μMs), 1.5 μ ldNTPs (2.5mM), 2.0 μ l10 × PCRBuffer are (containing 15mMMg
2+), 0.2 μ lTaq enzyme (5U/ μ l) and 11.7 μ lddH
2o.
Reaction conditions is the same.
If amplified production is DNA fragmentation A and B, then soybean to be measured be or candidate for 100-grain weight high, be designated as " H ";
If amplified production is DNA fragmentation A or B, then soybean to be measured be or candidate for 100-grain weight low, be designated as " L ".
Result is as Fig. 1, wherein, by DNA fragmentation type, detected materials is divided into A, B, H tri-groups, numbering 4 is followed successively by A group, 5, 8, 9, 10, 11, 12, 14, 15, 18, 21, 24, 29, 34, 36, 37, 44, 51, 52, the family of 53, the numbering that in B group, swimming lane is from left to right followed successively by this colony is respectively 13, 25, 30, 31, 32, 33, 38, 39, 40, 41, 45, 47, 49, 54, 59, 69, 70, 71, 72, the family of 76, the numbering that in H group, swimming lane is from left to right followed successively by this colony is respectively 1, 2, 3, 6, 7, 16, 17, 19, 20, 22, 23, 26, 27, 28, 35, 42, 43, 46, 48, 50, 56, 57, 58, 61, 62, 63, 64, 62, 66, 67, 68, 73, 75, 77, 78, 79, 81, 82, 83, the family of 84.Wherein unanimously with soybean varieties Ji beans No. 12 banding patterns represent with alphabetical A, consistent with wild soybean ZYD2738 banding pattern represents with letter b;
Result in figure is summed up as shown in table 1.
3, the factual survey of 100-grain weight height
Summer in 2011, at the F of Shijiazhuang field planting step 1
8126 family soybean in generation, randomized block design, each family kind 1 row, long 2.0 meters of row, line-spacing 0.5 meter, if 3 times are repeated.Manage under normal water and fertilizer condition, the 100-grain weight adding up each family and parent namely from soybean kernel is ripe and water content is 12% time hundred soybean kernels weight, calculates the mean value of three repetitions; Be that the soybean to be measured of 10-13g is defined as the high soybean of 100-grain weight by 100-grain weight, be designated as " H ", be that the soybean to be measured of 5-8g is defined as the low soybean of 100-grain weight, be designated as 100-grain weight " L ", result is if " measured result " of table 1 is shown in hurdle.
Predicting the outcome of table 1. soybean 100-grain weight height to be measured
Note: the soybean to be measured being numbered numeral in table be with Ji beans 12 be female parent, the ZYD2738 F that is male parent
8in generation, remains different strain in colony of heterozygosis system.
Result shows, to increase soybean to be measured with primer Barcsoyssr_13_890, predicts that in the 100-grain weight height of 126 soybean strains, have 119 strains to conform to measured result, accuracy rate is 94.44% by PCR primer or its band analysis.
Claims (10)
1. the method for a qualification or assistant identification soybean 100-grain weight height, comprise the genomic dna with Barcsoyssr_13_890 primer pair pcr amplification soybean to be measured, detect PCR primer, if the product of described pcr amplification is DNA fragmentation A and DNA fragmentation B, then described soybean to be measured is soybean that 100-grain weight is high or candidate is the soybean that 100-grain weight is high; If the product of described pcr amplification is described DNA fragmentation A or B, then described soybean to be measured is soybean that 100-grain weight is low or candidate is the soybean that 100-grain weight is low;
The primer pair that described Barcsoyssr_13_890 primer pair is made up of the single stranded DNA shown in the single stranded DNA shown in sequence 1 and sequence 2,
The PCR primer that the genomic dna that described DNA fragmentation A is No. 12, the primer pair pcr amplification the cultivated soybean kind Ji beans with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains;
The PCR primer that the genomic dna that described DNA fragmentation B is the primer pair pcr amplification wild soybean ZYD2738 with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains.
2. method according to claim 1, is characterized in that: described 100-grain weight is high refers to that the 100-grain weight of described soybean to be measured is 10-13g;
Described 100-grain weight is low refers to that the 100-grain weight of described soybean to be measured is 5-8g.
3. method according to claim 1 and 2, is characterized in that: described soybean to be measured is No. 12, soybean varieties Ji beans, wild soybean ZYD2738 or by the derivative kind of No. 12, described Ji beans and wild soybean ZYD2738 or strain.
4. method according to claim 3, is characterized in that: the described kind derivative with wild soybean ZYD2738 by No. 12, Ji beans or product are that No. 12, described Ji beans and wild soybean ZYD2738 are hybridized and multiplied to F
2for the above generation.
5. for the identification of or assistant identification soybean 100-grain weight height a DNA fragmentation, it is characterized in that: described DNA fragmentation is following 1) or 2) DNA fragmentation:
1) DNA fragmentation A and DNA fragmentation B;
2) DNA fragmentation A or DNA fragmentation B;
The PCR primer that the genomic dna that described DNA fragmentation A is No. 12, the primer pair pcr amplification the cultivated soybean kind Ji beans with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains;
The PCR primer that the genomic dna that described DNA fragmentation B is the primer pair pcr amplification wild soybean ZYD2738 with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains.
6. DNA fragmentation according to claim 5, is characterized in that:
Described 100-grain weight is high refers to that the 100-grain weight of described soybean to be measured is 10-13g;
Described 100-grain weight is low refers to that the 100-grain weight of described soybean to be measured is 5-8g.
Arbitrary described method or the application of the DNA fragmentation described in claim 5 or 6 in qualification or assistant identification soybean 100-grain weight height in 7.Barcsoyssr_13_890 primer pair or claim 1-4;
Or in Barcsoyssr_13_890 primer pair or claim 1-4 arbitrary described method or the DNA fragmentation described in claim 5 or 6 for the preparation of the application in qualification or assistant identification soybean 100-grain weight height low production.
Arbitrary described method or the application of the DNA fragmentation described in claim 5 or 6 in the soybean that the described 100-grain weight of screening is high in 8.Barcsoyssr_13_890 primer pair or claim 1-4;
Or arbitrary described method or the application of the DNA fragmentation described in claim 5 or 6 in the soybean that the described 100-grain weight of screening is low in Barcsoyssr_13_890 primer pair or claim 1-4;
Or arbitrary described method or the application of the DNA fragmentation described in claim 5 or 6 in soybean breeder in Barcsoyssr_13_890 primer pair or claim 1-4.
9. obtain the soybean method that 100-grain weight is high, comprise the steps:
1), No. 12, Ji beans and wild soybean ZYD2738 hybridize, and obtains hybrid generation, then selfing, obtains self-bred progeny;
2), with the genomic dna of Barcsoyssr_13_890 primer pair pcr amplification self-bred progeny soybean, detect PCR primer, if the product of described pcr amplification is DNA fragmentation A and DNA fragmentation B, then described soybean to be measured is soybean that 100-grain weight is high or candidate is the soybean that 100-grain weight is high;
The primer pair that described Barcsoyssr_13_890 primer pair is made up of the single stranded DNA shown in the single stranded DNA shown in sequence 1 and sequence 2,
The PCR primer that the genomic dna that described DNA fragmentation A is No. 12, the primer pair pcr amplification the cultivated soybean kind Ji beans with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains;
The PCR primer that the genomic dna that described DNA fragmentation B is the primer pair pcr amplification wild soybean ZYD2738 with the single stranded DNA composition shown in the single stranded DNA shown in sequence 1 and sequence 2 obtains.
10. method according to claim 9, is characterized in that:
Described 100-grain weight is high refers to that the 100-grain weight of described soybean to be measured is 10-13g;
Described self-bred progeny is F
2for the above generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410186304.1A CN105087757A (en) | 2014-05-05 | 2014-05-05 | Molecular marker for identifying hundred-grain weight of soybeans and application of molecular marker |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410186304.1A CN105087757A (en) | 2014-05-05 | 2014-05-05 | Molecular marker for identifying hundred-grain weight of soybeans and application of molecular marker |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105087757A true CN105087757A (en) | 2015-11-25 |
Family
ID=54569085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410186304.1A Pending CN105087757A (en) | 2014-05-05 | 2014-05-05 | Molecular marker for identifying hundred-grain weight of soybeans and application of molecular marker |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105087757A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543222A (en) * | 2016-02-29 | 2016-05-04 | 南京农业大学 | Molecular marker InDeL_33 of main-effect QTL (quantitative trait locus) of soybean hundred-grain weight and application of molecular marker InDeL_33 |
| CN108411021A (en) * | 2018-03-13 | 2018-08-17 | 山东省农业科学院作物研究所 | A kind of relevant molecule labelling method of soybean 100-grain weight and its label combination |
| CN113493851A (en) * | 2020-03-19 | 2021-10-12 | 河北省农林科学院粮油作物研究所 | Application of 32 soybean InDel markers in detection of soybean genetic diversity |
| CN116479164A (en) * | 2023-05-09 | 2023-07-25 | 吉林省农业科学院 | SNP locus, molecular marker, amplification primer and application of SNP locus and molecular marker related to soybean hundred-grain weight and size |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103276054A (en) * | 2013-03-13 | 2013-09-04 | 安徽农业大学 | Primer for auxiliary detection of soybean hundred-grain weight, and detection method thereof |
-
2014
- 2014-05-05 CN CN201410186304.1A patent/CN105087757A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103276054A (en) * | 2013-03-13 | 2013-09-04 | 安徽农业大学 | Primer for auxiliary detection of soybean hundred-grain weight, and detection method thereof |
Non-Patent Citations (3)
| Title |
|---|
| SONG QIJIAN等: ""a bundance of SSR motifs and development of candidate polymorphic SSR markers（BARCSOYSSR_1.0）in Soybean"", 《CROP SCIENCE》 * |
| YA-NAN SUN等: ""Multi-environment mapping and meta-analysis of 100-seed weight in soybean"", 《MOLECULAR BIOLOGY REPORTS》 * |
| 闫龙: ""大豆种间杂交（Glycine max × G. soja）后代籽粒性状QTL定位"", 《中国博士学位论文全文数据库 农业科技辑》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543222A (en) * | 2016-02-29 | 2016-05-04 | 南京农业大学 | Molecular marker InDeL_33 of main-effect QTL (quantitative trait locus) of soybean hundred-grain weight and application of molecular marker InDeL_33 |
| CN105543222B (en) * | 2016-02-29 | 2019-05-07 | 南京农业大学 | The molecular labeling InDeL_33 of soybean 100-grain weight main effect QTL and its application |
| CN108411021A (en) * | 2018-03-13 | 2018-08-17 | 山东省农业科学院作物研究所 | A kind of relevant molecule labelling method of soybean 100-grain weight and its label combination |
| CN113493851A (en) * | 2020-03-19 | 2021-10-12 | 河北省农林科学院粮油作物研究所 | Application of 32 soybean InDel markers in detection of soybean genetic diversity |
| CN113493851B (en) * | 2020-03-19 | 2022-07-12 | 河北省农林科学院粮油作物研究所 | Application of 32 soybean InDel markers in detection of soybean genetic diversity |
| CN116479164A (en) * | 2023-05-09 | 2023-07-25 | 吉林省农业科学院 | SNP locus, molecular marker, amplification primer and application of SNP locus and molecular marker related to soybean hundred-grain weight and size |
| CN116479164B (en) * | 2023-05-09 | 2024-02-13 | 吉林省农业科学院 | SNP locus, molecular marker, amplification primer and application of SNP locus and molecular marker related to soybean hundred-grain weight and size |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Luo et al. | Genetic diversity of mango cultivars estimated using SCoT and ISSR markers | |
| CN102154470B (en) | Molecular marker method for anti-rice blast gene Pi-hk1 (t) of paddy | |
| CN102766627B (en) | Molecular marker closely linked with oil content character of rapes and application | |
| CN103045588B (en) | Molecular marker of major QTL (Quantitative Trait Locus) of soybean seed protein content and application thereof | |
| Tsai et al. | Cultivar identification and genetic relationship of mango (Mangifera indica) in Taiwan using 37 SSR markers | |
| CN105525008A (en) | SSR molecular marker linked with wheat spikelet number QTL and application of SSR molecular marker | |
| CN104313018B (en) | The Indel marks of cucumber fruits thorniness gene ns and its application | |
| CN104498486B (en) | The Indel labelling of anti-cucumber powdery mildew gene pm h and its application | |
| CN105087757A (en) | Molecular marker for identifying hundred-grain weight of soybeans and application of molecular marker | |
| CN111500762B (en) | Sagittaria trifolia SSR primer group and application thereof | |
| CN104178560A (en) | Molecular marking method of rice stigma exsertion major QTL sites | |
| CN109321671A (en) | Wheat stripe rust resisting ospc gene QYr.sicau-1B-1 SSR molecular marker and application | |
| CN110331217B (en) | Microsatellite marker paternity test primer suitable for Nile tilapia, Oreochromis aureus and hybrid thereof, method and application | |
| CN106048012A (en) | Molecular marker for aiding selection of Rf (fertility restorer) gene, specific primers and application | |
| CN108753987A (en) | A kind of method of the general microsatellite Multiplex fluorescent PCR of silver carp flathead | |
| CN103773864B (en) | A kind of SNP marker method of pear fruit transverse diameter main effect QTL site and application thereof | |
| CN106222262B (en) | Primer pair and its identify rice nitrogen efficiently utilize the application in gene NRT1.1B genotype | |
| CN110106279A (en) | Unit point SSR primer sets and its application based on the exploitation of siberian wildrye genome sequence | |
| CN105063201B (en) | The molecular labeling of corn Chromosome 9 tassel row number main effect QTL and its application | |
| CN102080080A (en) | Molecular marker for assisted selective breeding of upland cotton with greensickness resistant traits | |
| CN105695454A (en) | Molecular marker for identifying sesame male sterile line of sesame and identifying method thereof | |
| CN108531642A (en) | One group of SSR molecular marker and its application for differentiating corn variety | |
| CN105420354B (en) | Conventional rice variety Huaihe River rice No. 5 and Huaihe River rice No. 18 identification methods based on InDel label | |
| CN102978288B (en) | Molecular marker for identifying soybean flowering period and application of molecular marker for identifying soybean flowering period | |
| CN107164481A (en) | Powdery mildew of melon correlation SSR marker and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151125 |
|
| RJ01 | Rejection of invention patent application after publication |