CN105080026A - Multifunctional and broad-spectrum active enzyme biological leaf fertilizer and preparation method thereof - Google Patents
Multifunctional and broad-spectrum active enzyme biological leaf fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention discloses active enzyme biological leaf fertilizer. The active enzyme biological leaf fertilizer is mainly composed of first bacteria (Arthrobacter sclerromae Huang et al.ACCC02953) generating peroxidase, second bacteria (Aurantimonas altamirensis juradoet al.ACCC01098) generating peroxidase, first fungi (moniliforme Sheldon ACCC30175) generating laccase, second fungi (Penicillium citrinum Thom penicillium citrinum ACCC30485) generating laccase, first actinomycetes (Streptomyces laurentii Trejo et al with the original number of CAH3.NAECC1210) generating tyrosinase, second actinomycetes ('Streptomyces jingyangensis'Tao et al.ACCC40021) generating tyrosinase, and enzymes Rubisco. By means of the leaf fertilizer, hazardous chemical substances on the surfaces of crops can be eliminated, toxin transferred into human bodies and fruit tissue can be blocked, contaminated crops containing toxin can be restored to the original non-toxic state, and soil containing toxic substances can be restored to the original non-toxic state. Meanwhile, the effect of increasing the yield of the crops is achieved.
Description
Technical field
The present invention relates to a kind of agricultural fertilizer class foliage fertilizer, particularly relate to organized enzyme biological foliage fertilizer of a kind of multi-functional, broad spectrum activity and preparation method thereof.
Background technology
Along with the development of biotechnology, increasing people starts to pay close attention to biological field, starts to be familiar with biotechnology in the important function of preserving the ecological environment and in agricultural production.Biotechnology be not only related to agricultural sustainable development, be also related to living environment and the grain security of the mankind, the longevity of the mankind and the quality of environment very close.Use in order to agricultural produce the quantity of the chemicals such as fertilizer, agricultural chemicals and herbicide just greatly to increase, and along with industrial expansion, cause toxic pollutant constantly to spread, cause huge harmful effect therefore to the environment such as soil, water body and air.
About the use of agricultural chemicals and the relation of environmental protection; many scientists are arguing the infringement problem about reality and potentiality intensely; but there is a fact to be apparent; even if press recommendation amount to use; some agricultural chemicals particularly pesticide, really can retain considerable time and constitute the pollution to soil and water after a procedure.In some instances, although working concentration is little, it is also a serious threat.Such as, the drinox of 1mg/kg is had also can to cause the death of 100% in the food of the every ration of quail.
According to the United Nations's statistics, the whole world has the discarded object of 20,000,000,000 tons of various pollutions and 1,000,000,000 tons of chemical substances to enter river and ocean every year.A large amount of discharges of industrial waste gas methane, carbon dioxide, sulfur dioxide, make temperature of the earth rise 1.4 DEG C-1.8 DEG C, greenhouse effects make climatic deterioration, and pollute air, water source and soil.The whole world has 1,200,000,000 populations to cause a disease because of sewage according to incompletely statistics, every year dead 7,000,000 people.More than 70% city about 1,500,000,000, whole world population suck pollution air, have every day 800 people to die from atmosphere pollution, noxious material is full of the world.As: polycyclc aromatic compound, lindane, Niran, sarin, explosive (TNT), agricultural chemicals and the toxic chemicals such as CCT, BHC, Polychlorinated biphenyls.What every day was all serious endangers environment, and the feature of its harm is: 1. persistence is very strong.The chemical substance of poisoning can retain even decades more than ten years in environment and soil.2. toxicity complexity is various.People is caused a disease, to disable, carcinogenic.The millions of people of annual death.3. the chronic accumulation of noxious material.Harmful toxins and heavy-metal residual are detained in animal and plant body, also can go down with the transfer of food chain ring by ring.4. long-distance migration is fast with extensive.Harmful substance floats to the whole world, and each is local, comprises the arctic, the South Pole.For everything, if people are not controlled and are administered, the mankind just may by oneself create chemical substance become extinct.
According to incompletely statistics, whole world Prof. Du Yucang material has more than 1,100 ten thousand kinds, constantly increases in recent years, cause the multiple components of poisonous and harmful substances to increase nearly 1,000,000 kinds every year with the quantity increasing 3-4 hundred million tons, multiple kind and Composite, add the difficulty of removing, degraded, decomposition.
The U.S. and many developed countries, be all classified as by bioremediation technology as process organic pollution and list strong standardization program in.Owing to suffering the intrusion of various pollutant in environment, the enzyme that different pollutant also can be produced by different microorganisms is degraded.Therefore, the product of current development is all set about from genetic engineering, proceeds to decomposing the microbial gene of different toxic pollutant in another kind of microorganism, but eventually because of difficulty and to consume human and material resources excessive, therefore, product is not yet seen in generation.Existing product mostly comprises the combination of 3 ~ 4 kinds of bacteriums and 2 ~ 3 kinds of fungies.Now isolated a kind of fungi of about 60 kinds of organic pollutions of degrading, in addition, also have some products, they are not microbial inoculums, but some can promote to be called active factors or activator by materials that soil beneficial microorganism is produced and bred.They are mostly made up through fermentation of natural organic matter.Point out according to research reports, the independent or cooperation microbial inoculum of this series products uses, and effect is excellent.
At present look into new data from domestic technique information to react, existing scavenger or degradation agent, there is a kind of degradation agent and can only to degrade a kind of agricultural chemicals or a kind of harmful substance, some product and technology can only solve surperficial toxin, can't resolve inside plants toxin.
Summary of the invention
The object of this invention is to provide a kind of agricultural fertilizer class foliage fertilizer, organized enzyme biological foliage fertilizer of a kind of multi-functional, broad spectrum activity and preparation method thereof is especially provided, solve above-mentioned technical problem, it can degrading plant surface with harmful substance and block noxious material contained in the noxious material that shifts in body and soil effectively, plants and soil can be quickly recovered to nontoxic primordial condition, while degraded toxin, also there is the effect promoting plant yield-increasing.
Technical problem to be solved by this invention is achieved through the following technical solutions:
A kind of organized enzyme biological foliage fertilizer of multi-functional, broad spectrum activity, by any 3 kinds to the 6 kinds biology enzyme microbial inoculums be prepared from comprised in following two bacteriums, two fungies and/or two actinomyces, described mushroom is: bacterium one (ArthrobactersclerromaeHuangetal.2005 branch arthrobacterium ACCC02953), bacterium two (Aurantimonasaltamirensisjuradoetal.2006 Wilber Sánchez Amita rice draws the orange monad ACCC01098 in hole); Fungi one (moniliformeSheldon Fusorium moniliforme Sheldon (rice Fusarium moniliforme Sheld) ACCC30175), fungi two (PenicilliumcitrinumThom Penicillium citrinum ACCC30485); Actinomyces one (StreptomyceslaurentiiTrejoetal1979 Lao Lun streptomycete Agricultural University Of Nanjing agricultural environment Culture Collection: original number: CAH
3.NAECC1210), actinomyces two (" Streptomycesjingyangensis " Taoetal. Streptomyces jingyangensis ACCC40021).
The organized enzyme biological foliage fertilizer of described multi-functional, broad spectrum activity, also comprises Rubisco enzyme.
The organized enzyme biological foliage fertilizer of described multi-functional, broad spectrum activity, controls the C/N ratio in described independence or multi strain co cultivation base within 20: 1 to 30: 1.
The organized enzyme biological foliage fertilizer of described multi-functional, broad spectrum activity, also comprises the cellulose bacteria synthetic media that the described bacterium of preparation produces laccase; Prepare the Clostridium Pasteurianum synthetic media that described fungi produces peroxidase; Prepare the wood chip comprehensive PDA culture medium that described actinomyces produce Tyrosinase.
The organized enzyme biological foliage fertilizer of described multi-functional, broad spectrum activity, described mushroom formulation weight part ratio is: bacterium No. one and bacterium No. two account for 10-30, and actinomyces microbial inoculum accounts for 20-40, and fungal inoculant accounts for 30-50, and Rubisco enzyme accounts for 5-15%.
The organized enzyme biological foliage fertilizer of described multi-functional, broad spectrum activity, described mushroom formula rate is: bacterium No. one and bacterium No. two account for 20, actinomyces microbial inoculum 30, and fungal inoculant accounts for 40, Rubisco enzyme and accounts for 10.Be (volume ratio or weight ratio?)
The organized enzyme biological foliage fertilizer of described multi-functional, broad spectrum activity, described Bacteria liquid containing bacterium number be 200,000,000/gram; Actinomyces containing bacterium number 200,000,000/gram; Fungi containing bacterium number 100,000,000/gram.
The organized enzyme biological foliage fertilizer of described multi-functional, broad spectrum activity, also comprises the total amount of adding aseptic Xi liquid glucose 10 times, carries out mixed culture fermentation; Described aseptic Xi liquid glucose is weight portion sterilized water 85-95 and molasses 5-15 mixture.
The present invention has following technique effect:
1, the present invention's organized enzyme biological foliage fertilizer that is multi-functional, broad spectrum activity is that the organized enzyme utilizing several microorganism to produce decomposes noxious material, in the production process of enzyme, microorganism can utilize again the composition of these pollutants, as the phosphorus source energy produced needed for enzyme and nitrogenous source nutrition, at mutual Co metabolism on, fast by organophosphor, the noxious materials such as organochlorine become carbon dioxide or remove toxin.
2, foliage fertilizer of the present invention is a kind of active enzyme biological resuming agent with broad spectrum activity, it is the mixture of several microbial inoculum enzyme, degraded and detoxicating comprehensively can be given to toxin such as organochlorine, organophosphor, organic nitrogen and organosulfur compounds simultaneously, in releasing toxin, there is very wide broad spectrum activity function.
3, foliage fertilizer of the present invention maintains enzymatic activity, the size of enzymatic activity and the length of time, is the commercial important technology core of organized enzyme biological foliage fertilizer of the present invention.The microorganism of organized enzyme and symbiosis also claims promotive factor or trophic factors, has good stability and continuous performance effect.Another important factor controls in the scope of 20: 1 to 30: 1 by foliage fertilizer C/N ratio, inorganic nitrogen can be made to be converted into the effect of organic nitrogen in organism comparatively obvious; Redundance nitrogen can be made also to be absorbed into as organic nitrogen and not easily to lose; Also can be and prevent nitrogen and organized enzyme loss from providing and very effectively ensure.Create an adapt circumstance so also to organized enzyme and microorganism thereof, ensure that longer biological effect.
Detailed description of the invention
Embodiment 1
A kind of organized enzyme biological foliage fertilizer of multi-functional, broad spectrum activity, comprise the organized enzyme of three kinds of energy fast decoupled noxious materials, mainly 1. laccase: by actinomyces one (StreptomyceslaurentiiTrejoetal1979 labor human relations streptomycete, Agricultural University Of Nanjing's agricultural environment Culture Collection: original number: CAH
3.NAECC1210; Can be hydrolyzed chitin in 30h, enzymatic activity reaches 126.79U; Be separated place: Zhejiang; Culture medium: 0012; Cultivation temperature: 30 DEG C.), actinomyces two (" Streptomycesjingyangensis " Taoetal. Streptomyces jingyangensis ACCC40021 ← China Agricultural University; 5406; Produce the basic element of cell division and Antibiotics material, produce with antibiotic fertilizer; Separation source: soil; Be separated place: Jingyang, Shanxi; Culture medium: 0012; Cultivation temperature: 28-30 DEG C.) produced, this enzyme exists with glucose proteins form;
itsevery a part can contain 3-4 copper molecule, and laccase also available 2.5 1 dimethylanilines (2.5-xylidine) induction produces a large amount of laccase A and laccase B;
2. peroxidase, by bacterium one ArthrobactersclerromaeHuangetal.2005 branch arthrobacterium (ACCC02953 ← INST OF AGRICULTURAL RESOURCES; Original number: GD-66-1-1,7023; Can be used to the bio-feritlizer of development & production; Separation source: soil; Be separated place: Inner Mongol Yakeshi; Culture medium: 0065; Cultivation temperature: 28 DEG C); Bacterium two Aurantimonasaltamirensisjuradoetal.2006 Wilber Sánchez Amita rice draws hole orange monad (ACCC01098 ← INST OF AGRICULTURAL RESOURCES; Original number: GD-10-2-1; Have and produce bio-feritlizer potentiality; Be separated place: Chaoyang District, Beijing City; Culture medium: 0002; Cultivation temperature: 28 DEG C) produced, can oxidation of phenol, the compounds such as aromatic amine, can also oxidation of alkyl per-compound and oxidized aromatic peracetic acid;
3. Tyrosinase: (moniliformeSheldon Fusorium moniliforme Sheldon (rice Fusarium moniliforme Sheld) ACCC30175 ← clay fertilizer institute of the Chinese Academy of Agricultural Sciences ← Chinese Academy of Agricultural Sciences atom institute ← Institute of Microorganism, Academia Sinica AS3.752 produces gibberellin by fungi one; Culture medium: 0014; Cultivation temperature: 25-28 DEG C.), fungi two (PenicilliumcitrinumThom Penicillium citrinum ACCC30485 ← clay fertilizer institute of the Chinese Academy of Agricultural Sciences ← Guangdong Microbes Inst GIM3.351 ← IFFI ← Shanghai Industrial institute of microbiology of food fermentation institute of Ministry of Light Industry; Original number: M71; For enzymolysis inosinicacid, produce phosphodiesterase and 5-nucleotides; Culture medium: 0014; Cultivation temperature: 25-28 DEG C) produced; The conversion of its energy catalysis Tyrosine and the mono-phenol of 0-.These enzymes, under " Co metabolism effect ", can utilize some noxious material and agricultural chemicals etc. as the phosphorus source energy itself grown and nitrogenous source nutrition, toxic pollutant of effectively degrading, wherein have stronger degradation to 8 class, 77 kinds of toxic pollutants through test.
The present embodiment also adds and has the photosynthetic enzyme-Rubisco enzyme that increases production and improves productivity (1.5 diphosphoribolose carboxylase oxygenase), it is the main active catalytic enzyme earth utilizing photosynthesis fixed carbon original matter, it effectively can carry out energy conversion and energy flow, utilize photosynthesis and leaf gas to carry out exchange to regulate, utilize photosynthesis that the radiant energy of the sun is converted into chemical energy.It can when do not need to have additional nutrients or energy ezpenditure increase substantially the output of plant and algae, increase yield 20%, for C4-plant, amount of increase in production is larger.
The microorganism of screening has and produces that the speed of enzyme is fast, quantity is many and the active feature such as large, and the ability of removing organic pollution is also along with increasing.
The present embodiment comprises the following steps preparation microbial inoculum of the present invention:
1. filter out three class 6 and can produce laccase, the microorganism fungus kind of junket gastral cavity acid enzyme and peroxidase, they, in the course of reaction producing enzyme, have stronger capacity of decomposition to harmful chemical.Wherein, 2, bacterium, 2, actinomyces, fungi 2.
2. have selected the culture medium prescription that three suitable all kinds of bacterial classifications are produced:
Cellulose bacteria synthetic media:
Note: slant medium removes filter paper bar 2% agar and 2% sodium carboxymethylcellulose (CMCNa)
Clostridium Pasteurianum synthetic media:
Wood chip comprehensive PDA culture medium:
Wood chip 50 grams of glucose 20 grams
Potato 200 grams of potassium dihydrogen phosphates 3 grams
20 grams, magnesium sulfate 1.5 grams of agar
3. after product is successfully prepared substantially, then add enzyme-Rubisco enzyme (1.5 diphosphoribolose carboxylases are enzyme-added), it is photosynthetic catalyzing enzyme, plays production-increasing function to plant.
4. the mixing ratio of microbial inoculum.Because different mushroom speed of production under common environment is different, directly affect the function of microbial inoculum, so the microbial inoculum after all kinds of bacterial classification is independently cultivated, the formula when the present embodiment Mixed culture is: Bacteria liquid accounts for 20%, containing bacterium number be 200,000,000/gram; Actinomyces account for 30%, containing bacterium number 200,000,000/gram; Fungi accounts for 40%, containing bacterium number 100,000,000/gram; Rubisco enzyme accounts for 10%.Again through adding the total amount of aseptic Xi liquid glucose and 10 times, sterilized water 90%+ molasses 10% mixture, carrying out mixed culture fermentation, detecting its mushroom total content at the 5th day, classification toatl proportion number reaches requirement and is as the criterion, and finished product has been put.
The present embodiment provides organized enzyme biological foliage fertilizer to remove the result of the test of toxicity to residues of pesticides.Test and carry out at Xi Liu city four, Tianjin Jinghai County hamlet plantation Kidney bean temperature canopy.Test in 1 day May in 2006, all spray acephatemet, flolimat, parathion three kinds of highly toxic pesticide first to the process of trial zone and contrast, the present embodiment " organized enzyme biological foliage fertilizer " has been sprayed to treatment region again after spraying, May 8, May 14, May 22 sampled three times respectively.Each process and contrast each sampling three kilograms, send quality of vegetable supervision and inspection center of the Ministry of Agriculture to detect.Record each district output simultaneously:
The result of the test table of table 1 organized enzyme biological foliage fertilizer degrading pesticide residues
Table 2 organized enzyme foliage fertilizer is to Kidney bean yield effect result table
Result of the test shows: organized enzyme foliage fertilizer after spraying pesticide 22 days, three kinds of agricultural chemicals efficiency of degrading all reach 100%.And output adds 19.09%.
Embodiment 2
Preparation bacterium liquid
(1) bacterium classification is cultivated: comprise the slant tube Spawn incubation of bacterial classification, 6 bacterium independence shaking table triangular flask Liquid Culture, 10 liters of glass fermentation tank three class bacterium classification Mixed culture.
(2) bacterium liquid Mixed culture: the Xi liquid molasses after first sterilizing 900 liters adds in the fermentation tank of 1000 liters.Again by bacterium, actinomyces and fungi independently cultivate bacterium liquid by 20 liters, and the quantity of 30 liters and 50 liters joins in fermentation tank molasses liquid.4-5 days is cultivated at 25-26 DEG C of temperature.Detecting total bacteria count and reach 200,000,000/gram time, can stop heating, allow its Temperature fall.Temperature loads hold-up tank when being down to 15 DEG C, makes it deposit 20 days, carries out after fermentation process, then add stabilizing agent, then carries out finished product bottling warehouse-in.
(3) preparation process: comprise function bacteria selection, the cultivation of slant strains, liquid spawn triangular flask shaking table cultivated, the cultivation of 10 liters of glass fermentation tank list class bacterium liquid, 1000 liters of fermentation tank mixed culture fermentation, basin deposit 20 days, add stabilizing agent, bottling, warehouse-in.
Embodiment 3
The preparation of solid active enzyme bio-fertilizer
The industrialization of solid active enzyme bio-fertilizer, large scale production method has two kinds, and one is pocket type fermentation preparation, and two is pillar fermentation preparations.
(1) pocket type fermentation preparation: with high pressure resistant, high temperature resistant, micro-ventilative polyethylene plastic film, the volume made is the fermentation bag of the solid medium of 1-2 kilogram, each fermentation bag have removable, can be independent, a fermentation bag is a unit, there is flexible operation, preventing the advantage of massive pollution, is the good technical method of medium-sized production scale.
Specifically: prepare culture matrix by embodiment 1 formula rate, sealable ventilative (O is loaded
2) property polybag in, carry out stack steam sterilizing, temperature controls at more than 100C.Sterilizing 12 hours.When in bag, the temperature of culture matrix is down to 30 DEG C, by liquid spawn, with in inoculator injection bag type culture matrix, and with autocratic aseptic adhesive tape, injection orifice is closed.Again culture medium bag is put into culturing room to ferment, fermentation overall process is carried out in bag.Fermentation bag will be placed evenly ventilative, avoids stacking fermentation temperature in overstocked bag too high, and living contaminants occurs.In general maintenance bag, fermentation temperature is no more than 28 DEG C and is advisable.Fermentation overall process timing sampling detects Mass Control, when every gram of culture medium surpasses 2,000 ten thousand containing bacterium spore amount, also fermentation can be stopped, the accurate finished product fermented is taken out, be placed on not by natural air drying in the environment of sun direct projection, when moisture drops to 16%-17%, also can be used as finished product pack, namely the product installing bag is solid active enzyme bio-feritlizer.
(2) pillar fermentation preparation: be main body by the cylinder shape fermentation tank fixed, adopts top charging, bottom discharge, high-temp steam sterilizing mode.Air feed, inoculation, a kind of large scale production method of temperature control automated system.
Specifically: first use metal, it is thick that diameter 1-3 rice built by safety glass or plastics, the pillar fermentation tank of high 3-10 rice, and each one of close-type door is installed in top and bottom.By the culture medium that the solid for mulation according to organized enzyme biological foliage fertilizer is worked good, from pillar fermentation tank top, fill it up with fermentation tank, again high steam is pumped in the steam comb in fermentation tank, by comb, HCS is carried out high-pressure and high-temperature steam sterilizing to culture medium uniformly, general culture medium material temperature, more than 100 DEG C, also can reach sterilizing requirement in 8-10 hour.Pass into cold air aseptic after filtering again, when material temperature being dropped to 30 DEG C, inoculate.Access having cultivated liquid spawn in culture medium uniformly ferment by inoculation pipe-line system.Sweat temperature is regulated by the aseptic cold air after filtering.Maintain the temperature at 25 DEG C-28 DEG C.Fermentation medium containing after bacterium spore amount reaches 2,000 ten thousand every gram, pillar fermenter base is opened, the solid fertilizer fermented is taken out, be put into shady and cool dry place and carry out natural air drying, stop when humidity drops to 16%-17%, pack, also can become commodity-type bioactive enzyme solid fertilizer.
Claims (9)
1. the organized enzyme biological foliage fertilizer of multi-functional a, broad spectrum activity, by any 3 kinds to the 6 kinds biology enzyme microbial inoculums be prepared from comprised in following two bacteriums, two fungies and/or two actinomyces, described mushroom is: bacterium one (ArthrobacterscierromaeHuangetal.2005 branch arthrobacterium ACCC02953), bacterium two (Aurantimonasaltamirensisjuradoetal.2006 Wilber Sánchez Amita rice draws the orange monad ACCC01098 in hole); Fungi one (moniliformeSheldon Fusorium moniliforme Sheldon (rice Fusarium moniliforme Sheld) ACCC30175), fungi two (PenicilliumcitrinumThom Penicillium citrinum ACCC30485); Actinomyces one (StreptomyceslaurentiiTrejoetal1979 Lao Lun streptomycete Agricultural University Of Nanjing agricultural environment Culture Collection: original number: CAH
3.NAECC1210), actinomyces two (" Streptomycesjingyangensis " Taoetal. Streptomyces jingyangensis ACCC40021).
2. the organized enzyme biological foliage fertilizer of multi-functional, broad spectrum activity according to claim 1, also comprises Rubisco enzyme.
3. the organized enzyme biological foliage fertilizer of multi-functional according to claim 1 or 2, broad spectrum activity, controls the C/N ratio in described independence or multi strain co cultivation base within 20: 1 to 30: 1.
4. the organized enzyme biological foliage fertilizer of multi-functional according to claim 1 or 2, broad spectrum activity, also comprises the cellulose bacteria synthetic media that the described bacterium of preparation produces laccase; Prepare the Clostridium Pasteurianum synthetic media that described fungi produces peroxidase; Prepare the wood chip comprehensive PDA culture medium that described actinomyces produce Tyrosinase.
5. the organized enzyme biological foliage fertilizer of multi-functional according to claim 1 or 2, broad spectrum activity, described mushroom formulation weight part ratio is: bacterium No. one and bacterium No. two account for microbial inoculum 10-30, actinomyces microbial inoculum accounts for 20-40, and fungal inoculant accounts for 30-50, and Rubisco enzyme accounts for 5-15%.
6. the organized enzyme biological foliage fertilizer of multi-functional, broad spectrum activity according to claim 3, bacterium No. one and bacterium No. two account for microbial inoculum 10-30, and actinomyces microbial inoculum accounts for 20-40, and fungal inoculant accounts for 30-50, and Rubisco enzyme accounts for 5-15%.
7. the organized enzyme biological foliage fertilizer of multi-functional, broad spectrum activity according to claim 4, described mushroom formula rate is: bacterium No. one and bacterium No. two account for 20, actinomyces microbial inoculum 30, and fungal inoculant accounts for 40, Rubisco enzyme and accounts for 10.Be (volume ratio or weight ratio?).
8. the organized enzyme biological foliage fertilizer of multi-functional, broad spectrum activity according to claim 7, described Bacteria liquid containing bacterium number be 200,000,000/gram; Actinomyces containing bacterium number 200,000,000/gram; Fungi containing bacterium number 100,000,000/gram.
9. the organized enzyme biological foliage fertilizer of multi-functional according to claim 1 or 2, broad spectrum activity, also comprises the total amount of adding aseptic Xi liquid glucose 10 times, carries out mixed culture fermentation; Described aseptic Xi liquid glucose is weight portion sterilized water 85-95 and molasses 5-15 mixture.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105060951A (en) * | 2015-09-14 | 2015-11-18 | 北京晨奥润泽科技股份有限公司 | Active-enzyme biological leaf fertilizer and preparation method thereof |
| CN115385744A (en) * | 2022-09-13 | 2022-11-25 | 新疆益果生物科技有限责任公司 | Apple calcium alginate water-soluble leaf fertilizer for Xinjiang saline-alkali soil and preparation method thereof |
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2014
- 2014-05-19 CN CN201410208779.6A patent/CN105080026A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105060951A (en) * | 2015-09-14 | 2015-11-18 | 北京晨奥润泽科技股份有限公司 | Active-enzyme biological leaf fertilizer and preparation method thereof |
| CN115385744A (en) * | 2022-09-13 | 2022-11-25 | 新疆益果生物科技有限责任公司 | Apple calcium alginate water-soluble leaf fertilizer for Xinjiang saline-alkali soil and preparation method thereof |
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