CN105077214A - Preparation method of selenium-enriched agaricus blazei - Google Patents
Preparation method of selenium-enriched agaricus blazei Download PDFInfo
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- CN105077214A CN105077214A CN201510452765.3A CN201510452765A CN105077214A CN 105077214 A CN105077214 A CN 105077214A CN 201510452765 A CN201510452765 A CN 201510452765A CN 105077214 A CN105077214 A CN 105077214A
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- selenium
- agaricus blazei
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- 239000011669 selenium Substances 0.000 title claims abstract description 88
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 78
- 241001327634 Agaricus blazei Species 0.000 title claims abstract description 59
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000009630 liquid culture Methods 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 229940091258 selenium supplement Drugs 0.000 claims description 69
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 8
- 229960001471 sodium selenite Drugs 0.000 claims description 8
- 239000011781 sodium selenite Substances 0.000 claims description 8
- 235000015921 sodium selenite Nutrition 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 238000001479 atomic absorption spectroscopy Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 230000009514 concussion Effects 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 239000012533 medium component Substances 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 8
- 241000233866 Fungi Species 0.000 abstract description 6
- 229920001282 polysaccharide Polymers 0.000 abstract description 6
- 239000005017 polysaccharide Substances 0.000 abstract description 6
- 239000013589 supplement Substances 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 235000014106 fortified food Nutrition 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241000206575 Chondrus crispus Species 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 150000003346 selenoethers Chemical class 0.000 description 3
- 208000030507 AIDS Diseases 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 208000019926 Keshan disease Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 238000012364 cultivation method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- -1 monohydrate selenium dioxide ester polysaccharide Chemical class 0.000 description 2
- 201000008482 osteoarthritis Diseases 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241001474374 Blennius Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 150000003342 selenium Chemical class 0.000 description 1
- 229940065287 selenium compound Drugs 0.000 description 1
- 150000003343 selenium compounds Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 231100000701 toxic element Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention discloses a preparation method of selenium-enriched agaricus blazei, and belongs to the technical field of selenium-enriched food preparation. The preparation method consists of the following steps: a, agaricus blazei mycelia inoculation: inoculating agaricus blazei mycelia to liquid medium with selenium concentrations of 0, 20 mug/g, 40 mug/g, 80 mug/g and 160 mug/g, respectively; b, mycelium liquid culture: placing the inoculated medium in a constant temperature shaker incubator (28-35 DEG C), and conducting shake culture for 3-10 days; c, mycelium collection: filtering the cultured bacteria liquid through a sieve, and collecting the mycelia; d, pure water rinse: rinsing the mycelia with pure water; and e, drying: placing the rinsed agaricus blazei mycelia in a bake oven for drying, thereby obtaining the selenium-enriched agaricus blazei. The preparation method can effectively improve the selenium enrichment rate in fungi, and the selenium content of the liquid medium cultured agaricus blazei is much higher than that of the normal method cultured agaricus blazei. The obtained selenium-enriched agaricus blazei can supplement selenium and can also effectively supplement polysaccharide substances at the same time, and can improve the immunity of the human body.
Description
Technical field
The invention belongs to selenium-enriched food preparing technical field, especially relevant with a kind of preparation method of rich-selenium Agaricus blazei.
Background technology
Since Sweden chemist Berzelius in 1817 has found elemental selenium, selenium has been considered to toxic element always, until nineteen fifty-seven Schwarz and Foctz has found the trophism of selenium in the research of right/Factor30.Particularly in recent years, along with the development of molecular biology, medical science and nutrition, the trophism of selenium is more and more subject to people's attention, and one of micronutrient element being listed in needed by human.Increasing research shows, selenium is relevant with numerous disease, as Kaschin-Beck disease, Keshan disease, cancer etc.People keeps appropriate selenium to take in throughout the year, have protection biomembrane, eliminate free radical, anti-ageing, promote the effects such as immunologic function, treatment and control action are played to cancer, cardiovascular disease, AIDS etc.
At present, China has the county of 22% (city) to belong to scarce selenium or low selenium area, and the population of 2/3 exists selenium insufficiency of intake in various degree, and animal and human's body lacks selenium situation aggravates.Therefore, China has started one and has mended selenium heat in recent years, and selenium-enriched food has become one of focus of current functional food research and development.
At present, rich selenium hardening agent mainly contains three kinds: sodium selenite, Se-enriched yeast, selenide of carragheen, and sodium selenite is a kind of selenium salt, and price is very cheap, and even if a lot of selenium product of mending selects this inorganic selenium as selenium source, but inexcessive, is also poisonous, needs careful use; Selenide of carragheen: (also known as seaweed selenium poly saccharide, monohydrate selenium dioxide ester polysaccharide) is that the puritan filler polysaccharide ester extracted with marine algae does parent, adopt accurate molecular docking technology, make a kind of novel organic selenium compounds that trace elements of selenium closely engages in molecular state with active polysaccharide, but cost is high, supplier is few, limits the use of selenide of carragheen; Se-enriched yeast is a kind of organic selenium product, and it has biosynthesis, and raw material is sodium selenite, as long as biochemical indicator is qualified when detecting, sodium selenite content, in control range, is exactly safety product substantially.Yeast selenium is the best product of cost performance.Therefore, by conversion technology carry out selenium enrichment and transform be a kind of effectively and the method for safety, plant transformation techniques is mostly still relied on to realize at present, wherein water planting (or soil) executes selenium or crop spraying selenium, the content utilizing the biology of crop to transform to improve Organic Selenium and to enter that food chain eats for humans and animals be a main technique of enriched selenium.
Patent publication No. is the preparation method that CN103535186A discloses a kind of rich-selenium Agaricus blazei for food additives, comprise the following steps: (1) is mixed into the clay of load sodium selenite in common Agricus blazei planting material in 1-10% ratio, (2) cultivation of rich-selenium Agaricus blazei is carried out according to the normal breeding method of Agricus blazei, to obtain the Agricus blazei of low-heavy metal content, high Se content, but the accumulation rate of the content of high Se content for preparing of the method still in not high, fungi is not high yet.For this problem, applicant has been researched and developed a kind of preparation method utilizing fungi Agricus blazei to carry out the rich-selenium Agaricus blazei of selenium-enriched liquid cultivation and has been solved this problem.
Summary of the invention
For the problems referred to above, object of the present invention aims to provide and a kind ofly utilizes fungi Agricus blazei to carry out selenium-enriched liquid to cultivate and mending the preparation method that effectively can supplement the rich-selenium Agaricus blazei of polysaccharose substance while selenium.
For this reason, the present invention is by the following technical solutions: a kind of preparation method of rich-selenium Agaricus blazei, is characterized in that, comprises the following steps:
A, Mycelium in Agaricus blazei Murill are inoculated: Mycelium in Agaricus blazei Murill is inoculated into selenium concentration and is respectively in the fluid nutrient medium of 0,20 μ g/g, 40 μ g/g, 80 μ g/g, 160 μ g/g;
B, mycelium liquid are cultivated: the culture medium inoculated is placed in 28 ~ 35 DEG C of constant-temperature table incubators and carries out concussion cultivation 3 ~ 10 days;
C, mycelium are collected: filtered by cultured bacterium liquid sieve, collect mycelium;
D, clear water rinse: rinsed by mycelium with pure water;
E, oven dry: the Mycelium in Agaricus blazei Murill after flushing is placed in baking oven and dries.
As to technique scheme supplement and perfect, the present invention also comprises following technical characteristic.
The inoculum concentration of Mycelium in Agaricus blazei Murill described in step a is 10 ~ 30%.
The described medium component that in step a, Mycelium in Agaricus blazei Murill Liquid Culture is used and mass fraction are: potato 20%, wheat bran 2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.075% and sodium selenite appropriate.
In step b, Agricus blazei Liquid Culture temperature is 30 DEG C, and incubation time is 5 days.
20 ~ 120 mesh sieves are adopted to filter when Mycelium in Agaricus blazei Murill is collected in step c.
The mycelium purified rinse water gathered in steps d, washing time is 2 ~ 5 times.
The oven for drying temperature being placed in Mycelium in Agaricus blazei Murill in step e is 60 ~ 80 DEG C.
The rich-selenium Agaricus blazei obtained after oven dry is adopted the content of total selenium in Atomic Absorption Spectrometry Mycelium in Agaricus blazei Murill, thus obtain the rich-selenium Agaricus blazei of standard.
Use the present invention can reach following beneficial effect: the present invention adopts the method for Liquid Culture, effectively improves the accumulation rate of selenium in fungi, cultivate the Agricus blazei Se content that obtains far away higher than the Agricus blazei that common cultivation method obtains by fluid nutrient medium.The rich-selenium Agaricus blazei that the present invention obtains effectively can supplement polysaccharose substance while benefit selenium, can improve the immunity of human body.
Accompanying drawing explanation
Fig. 1 is process flow diagram of the present invention.
Fig. 2 is rich-selenium Agaricus blazei mycelium dry weight experimental data figure.
Detailed description of the invention
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail.
Embodiment: as shown in Figure 1, preparation method of the present invention comprises the following steps:
A, Mycelium in Agaricus blazei Murill are inoculated: Mycelium in Agaricus blazei Murill is inoculated into selenium concentration and is respectively in the fluid nutrient medium of 0,20 μ g/g, 40 μ g/g, 80 μ g/g, 160 μ g/g;
B, mycelium liquid are cultivated: the culture medium inoculated is placed in 28 ~ 35 DEG C of constant-temperature table incubators and carries out concussion cultivation 3 ~ 10 days;
C, mycelium are collected: filtered by cultured bacterium liquid sieve, collect mycelium;
D, clear water rinse: rinsed by mycelium with pure water;
E, oven dry: the Mycelium in Agaricus blazei Murill after flushing is placed in baking oven and dries.
Further, the inoculum concentration of Mycelium in Agaricus blazei Murill described in step a is 10 ~ 30%.The described medium component that in step a, Mycelium in Agaricus blazei Murill Liquid Culture is used and mass fraction are: potato 20%, wheat bran 2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.075% and sodium selenite appropriate.
Further, 20 ~ 120 mesh sieves are adopted to filter when Mycelium in Agaricus blazei Murill is collected in step c.
Further, the mycelium purified rinse water gathered in steps d, washing time is 2 ~ 5 times.
Further, the oven for drying temperature being placed in Mycelium in Agaricus blazei Murill in step e is 60 ~ 80 DEG C.
Further, the rich-selenium Agaricus blazei obtained after oven dry is adopted the content of total selenium in Atomic Absorption Spectrometry Mycelium in Agaricus blazei Murill, thus obtain the rich-selenium Agaricus blazei of standard.Rich-selenium Agaricus blazei laboratory test results is analyzed: weigh after drying in an oven, as shown in Figure 2, Agricus blazei dry yield under 40 gradient condition is the highest for experimental result, is about about 7.8% and illustrates that the growing way of Mycelium in Agaricus blazei Murill is best under this gradient; Under 80,160 gradient condition, dry yield is respectively 5.6%, 5.8%, more stable, and illustrate that the growth of Mycelium in Agaricus blazei Murill is in basicly stable state, growing way is better.
Determination of Selenium adopts the content of total selenium in Atomic Absorption Spectrometry mycelium.The calculating of Se accumulation rate:
Selenium concentration × 100% in rich selenium rate=(Se content in rich selenium mycelia-blank mycelia Se content) × bacterium dry weight/culture medium.
Selenium is relevant with numerous disease, as Kaschin-Beck disease, Keshan disease, cancer etc.People keeps appropriate selenium to take in throughout the year, have protection biomembrane, eliminate free radical, anti-ageing, promote the effects such as immunologic function, treatment and control action are played to cancer, cardiovascular disease, AIDS etc.The present invention utilizes famous and precious fungi Agricus blazei to carry out selenium-enriched liquid cultivation, obtains the Agricus blazei of high Se content, cultivates the Agricus blazei Se content that obtains far away higher than the Agricus blazei that common cultivation method obtains by fluid nutrient medium.Agricus blazei itself is containing abundant polysaccharide and each seed amino acid, and the rich-selenium Agaricus blazei that the present invention obtains effectively can supplement polysaccharose substance while benefit selenium, improves the immunity of human body
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (8)
1. a preparation method for rich-selenium Agaricus blazei, is characterized in that: comprise the following steps:
A, Mycelium in Agaricus blazei Murill are inoculated: Mycelium in Agaricus blazei Murill is inoculated into selenium concentration and is respectively in the fluid nutrient medium of 0,20 μ g/g, 40 μ g/g, 80 μ g/g, 160 μ g/g;
B, mycelium liquid are cultivated: the culture medium inoculated is placed in constant-temperature table incubator and carries out concussion cultivation;
C, mycelium are collected: filtered by cultured bacterium liquid sieve, collect mycelium;
D, clear water rinse: rinsed by mycelium with pure water;
E, oven dry: the Mycelium in Agaricus blazei Murill after flushing is placed in baking oven and dries.
2. the preparation method of a kind of rich-selenium Agaricus blazei according to claim 1, is characterized in that: the inoculum concentration of Mycelium in Agaricus blazei Murill described in step a is 10 ~ 30%.
3. the preparation method of a kind of rich-selenium Agaricus blazei according to claim 1, it is characterized in that: the described medium component that in step a, Mycelium in Agaricus blazei Murill Liquid Culture is used and mass fraction are: potato 20%, wheat bran 2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.075% and sodium selenite.
4. the preparation method of a kind of rich-selenium Agaricus blazei according to claim 1, is characterized in that: in step b, Agricus blazei Liquid Culture temperature is 28 ~ 35 DEG C, and incubation time is 3 ~ 10 days.
5. the preparation method of a kind of rich-selenium Agaricus blazei according to claim 1, is characterized in that: adopt 20 ~ 120 mesh sieves to filter when Mycelium in Agaricus blazei Murill is collected in step c.
6. the preparation method of a kind of rich-selenium Agaricus blazei according to claim 1, is characterized in that: the mycelium purified rinse water gathered in steps d, and washing time is 2 ~ 5 times.
7. the preparation method of a kind of rich-selenium Agaricus blazei according to claim 1, is characterized in that: the oven for drying temperature being placed in Mycelium in Agaricus blazei Murill in step e is 60 ~ 80 DEG C.
8. the preparation method of a kind of rich-selenium Agaricus blazei according to claim 1, is characterized in that: the content rich-selenium Agaricus blazei obtained after oven dry being adopted total selenium in Atomic Absorption Spectrometry Mycelium in Agaricus blazei Murill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510452765.3A CN105077214A (en) | 2015-10-16 | 2015-10-16 | Preparation method of selenium-enriched agaricus blazei |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510452765.3A CN105077214A (en) | 2015-10-16 | 2015-10-16 | Preparation method of selenium-enriched agaricus blazei |
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| CN105077214A true CN105077214A (en) | 2015-11-25 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684728A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Tricholoma matsutake liquid strain breeding method and field bionic cultivation method |
| CN108265011A (en) * | 2018-04-26 | 2018-07-10 | 杭州雪域生物技术有限公司 | Mycelium liquid fermentation medium composition of a kind of rich-selenium Agaricus blazei and preparation method thereof and fermentation process |
| CN108605654A (en) * | 2017-01-12 | 2018-10-02 | 湖南奇硒健康产业有限公司 | A kind of method of acclimation and screening selenium-rich gill fungus bacterium strain |
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| KR20050060725A (en) * | 2003-12-17 | 2005-06-22 | 주식회사 태평양 | Method for producing mycelium of agaricus blazei murrill containing organic selenium |
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2015
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684728A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Tricholoma matsutake liquid strain breeding method and field bionic cultivation method |
| CN108605654A (en) * | 2017-01-12 | 2018-10-02 | 湖南奇硒健康产业有限公司 | A kind of method of acclimation and screening selenium-rich gill fungus bacterium strain |
| CN108265011A (en) * | 2018-04-26 | 2018-07-10 | 杭州雪域生物技术有限公司 | Mycelium liquid fermentation medium composition of a kind of rich-selenium Agaricus blazei and preparation method thereof and fermentation process |
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