CN105053408B - A kind of preparation method of the prebiotic tea of high gamma-aminobutyric acid mulberry leaf - Google Patents

A kind of preparation method of the prebiotic tea of high gamma-aminobutyric acid mulberry leaf Download PDF

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CN105053408B
CN105053408B CN201510515033.4A CN201510515033A CN105053408B CN 105053408 B CN105053408 B CN 105053408B CN 201510515033 A CN201510515033 A CN 201510515033A CN 105053408 B CN105053408 B CN 105053408B
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mulberry leaf
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tea
gaba
nutrient solution
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陈瑕
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Abstract

The invention belongs to tea preparation fields, are specially a kind of preparation method of the high prebiotic tea of gamma aminobutyric acid mulberry leaf.This method is using high-quality protein mulberry leaf as raw material, with a kind of based on glutamic acid, the nutrient solution being combined with nitrogen, phosphorus, potassium, pass through the method for root outer spraying, generate a kind of GABA mulberry leaf of pure natural high-content, its content >=350mg -480mg/100g, lysine content are up to 1233mg/100g.With chicory extract-inulin(Liquid)It is mixed with GABA mulberry leaf, pile fermentation generates probiotics.The prebiotic tea of mulberry leaf rich in high GABA is made of distinctive tea-manufacturing technology.It is decaffeinated.Collect the high-quality mulberry leaf of high protein, the different physiological roles such as high GABA and probiotics, melt nutrition, health care, food, dietotherapy, medical treatment, prevention in the tea culture of one.It is of low cost using resources advantage, full of nutrition, fragrant in taste.Extend silkworm and mulberry chain, improve industry comprehensive benefit.

Description

A kind of preparation method of the prebiotic tea of high gamma-aminobutyric acid mulberry leaf
Technical field
The invention belongs to tea preparation fields, are specially a kind of preparation method of the prebiotic tea of high gamma-aminobutyric acid mulberry leaf.
Background technology
Gamma-aminobutyric acid (γ-Aminodutrc20ia) is a kind of four carbon of one kind by L-glutamate decarboxylase generation The natural amino acid (abbreviation GABA) of nonprotein.Molecular formula is H2N- CH2CH2-CH2- COOH, relative molecular weight 103.It is Important inhibition neurotransmitter in a kind of nervous system.It is widely present in prokaryotes and eucaryote, exists only in nerve In tissue, the content of mesencephalic tissue is 0.1-0.6mg/g.With the metabolism and aging of human body cell and the influence of disease, make Continuous reduction reduce, it is necessary to supplemented by food.The highest region of concentration is the black substance in brain, and the GABA of intracerebral is by paddy Propylhomoserin decarboxylase and formed.
GABA participates in a variety of physiological metabolism activities of antibody, has very high physiological activity.It is a kind of neurotrophic factor, Brain vigor can be enhanced, nourishing brain cell has the function of nervous physiology function drug and raw-food material.It is usually used in treating hepatic coma With various diseases caused by cerebrovascular disorder, spiritedness is stabilized, reduces blood pressure, expands blood vessel, promote brain blood flow, adjust physiology Function, improve blood fat, anti-arrhythmia, antifatigue, enhancing is immune, pre- preventing obesity, senile dementia, cardiovascular and cerebrovascular disease and Pa Jin Prosperous third constellations disease.
Health ministry in 2009 is approved as new resource food, and state food safety standard is GB2760, is classified as country and permits Perhaps the food used, for natural perfume material list.Requirement is 30-100mg for each person every day.
Japan extracts GABA from rice germ, and complex process is of high cost, expensive.Contain in Activities of Some Plants GABA, but content is little, and the content of human physiological functions needs is not achieved.Therefore, research is improved with physiological activity content The species and food of pure natural GABA have a very important significance.
In terms of current substantial amounts of research all concentrates on tealeaves, take mostly at enrichment method and the nitrogen pressure oxygen from tree immersion The method of reason also has chemical synthesis.The maximum deficiency of these methods is not natural enough, and complex process is of high cost, and effect is low, And containing caffeine, so all not ideal enough.
Mulberry leaf are used as medicine with a long history, have and enrich blood, beneficial liver, logical brain, clear away heart-fire, improving eyesight, and hypoglycemic, lipid-loweringing, diuresis, disappear at decompression The drug effects such as inflammation, cough-relieving, long hair.It can improve blood supply in brain amount, enhance brain activity, increase growth hormone secretion, be good for liver kidney-nourishing, Improve climacteric syndrome, medicinal function is peculiar.Health ministry is formally included in the catalogue of " be both food and drug ".
The content of the invention
The purpose of the present invention is being directed to more than technical problem, provide it is a kind of according to biotransformation method, with high-quality protein mulberry leaf For raw material, root is carried out by the nutrient solution based on paddy amino acid, supplemented by nitrogen, phosphorus, potassium and is segmented the method sprayed outside, utilizes plant The photosynthesis and day and night temperature of object realize biological concentration conversion, improve a kind of high gamma-aminobutyric acid mulberry leaf benefit of GABA content The preparation method of raw tea.
To achieve these goals, the specific technical solution of the present invention is:
A kind of preparation method of the prebiotic tea of high gamma-aminobutyric acid mulberry leaf, comprises the following steps:
The first step selects high-quality protein mulberry leaf as raw material, and the protein containing 25%-45% in the dry matter of Ben Sangye is to plant Highest one kind containing protein in object leaf, being made after tealeaves to be digested and absorbs in portion greatly.It contains 18 kinds of amino acid, group The 44.85% of total amino acid content is accounted for into 8 kinds of identical with defatted soy flour, needed by human amino acid, is a kind of good plant egg White matter.The also minerals containing 20 multivitamins and calcium, iron, zinc, selenium, manganese etc..Modern research shows that it contains, there are many have pharmacology The substance of effect, such as steroidal and three patch class compounds, the chemistry such as chromocor compound, cumarin, volatile oil, alkaloid, organic acid Substance.Wherein 1-DNJ (DNJ) content is up to 100mg/100g, is a kind of a-glycosidase inhibitor, there have to be special Inhibit blood glucose and rise function.The content of phytosterol is 3-4 times of green tea.Also contain a kind of famous polyphenoils-quercitrin Glycosides, it can reduce carcinogenic activity, inhibit the growth of canceration enzyme, and energy anti-inflammatory, antibacterial, prevent the oxidation of low-density lipoprotein And destroy blood vessel.
Second step is prepared and using nutrient solution, improves the content in mulberry leaf, raw material is provided to prepare the prebiotic tea of high GABA. Since the preceding biomass of GABA is glutamic acid, this nutrient solution is aided with nitrogen, phosphorus, potassium based on sodium glutamate.It is given birth in mulberry leaf Long early period, i.e. 15 days to No. 20 April are sprayed, and spring slightly blade can be promoted loose;Growth medium is front and rear for " the Beginning of summer ", i.e. May No. 15 to No. 20 are sprayed, and can make summer mulberry riotous growth;Later growth is front and rear for " the beginning of autumn ", i.e. mid-August is sprayed, can Make mulberry leaf continued propagation, extend blade hardening and branch binds, promote autumn hair.The method sprayed with blade face segmentation, is allowed to be enriched with Conversion, excitation mulberry tree, the activity of mulberry leaf itself glutamate decarboxylase, by the photosynthesis of plant, day and night temperature carries out biology Conversion, generation and the content for improving GABA.And nitrogen is the main component of cellular plasm, protein, enzyme and chlorophyll, promotes battalion Health is long, improves Photosynthetic Efficiency;Phosphorus is the main component to form plasm and nucleus, promotes newborn device pipe growth, promotes and inhale Receipts ability promotes photosynthesis, hasting of maturity;Potassium is primary high-quality to maintaining cell, boosts metabolism and is closed with carbohydrate Into making loose into blade, improve the content of GABA.
1st, in nutrient solution each substance mass percentage:Sodium glutamate 1-6%, urea 0.3-0.5%, calcium superphosphate 0.5- 1.0%, potassium sulfate 0.3-0.5%, plant ash 1.0%, surplus are water, and gross mass percentage is 100%, can be configured with water 100kg into Row sprays.
2nd, the quantity sprayed:100-150 kilograms of nutrient solution is sprayed per acre.
3rd, the time sprayed:It is sprayed in the morning of fine day or dusk, the rainy day is unsuitable.
4th, the position sprayed:Mulberry leaf are to absorb nutrition by back side stomata, therefore, must nozzle is upward when spraying It is sprayed, nutrient solution is sprayed on the back side of mulberry leaf.
5th, the number sprayed:It is sprayed 3-4 times in blade each growth period, it can picking leaves throughout the year.
Fosterization is carried out by the bioconversion function of mulberry leaf itself to absorb, and is become inorganic to be organic.Improve mulberry leaf GABA The method of content, it is pure natural better than the method impregnated from tree, it is noresidue, pollution-free, nuisanceless, without any side effects.
The method that 3rd step is traditionally combined with modern technologies prepares the high prebiotic tea of GABA mulberry leaf.
1st, dedusting:In order to ensure the sanitation and hygiene of mulberry leaf, mulberry leaf must be washed with the water of sanitation and hygiene before picking It washs.Method is that the mulberry leaf of picking are carried out with spray clean with sprayer, removes dust, silt on blade, accomplishes sanitation and hygiene.
2nd, pick:Moisture on blade is picked after doing.Pick the mulberry leaf of fresh mature, it is desirable that tender, net, even, fresh Four big features, no dew.Between holding leaf under the arm with forefinger and thumb among children's stalk, the strength referred to by means of two takes leaf.
3rd, break and cut:The mulberry leaf plucked back are subjected to the disconnected small pieces cut, big blade is cut into 2cm × 10cm.
4th, stand is blue or green:Mulberry leaf after disconnected cut are spread and are dried in the air sun-drenched on seat, by sunlight or hot gas moisture are promoted to steam Hair, makes blade soften, and temperature is controlled at 28-32 DEG C, spreads out green grass or young crops 3-5h.
5th, finish:Mulberry leaf are put into water-removing pot, with the activity of enzyme in high temperature mulberry leaf, remove the peculiar smell in mulberry leaf, Reduce the partial moisture in mulberry leaf.Mulberry leaf after drying in the air are entered to finish in pot, the saline solution for being 0.3-0.5% with mass percentage, The quality of mulberry leaf and saline solution is 1:6,30s is impregnated with 100 DEG C of boiling saline solution, is then rinsed 2-3 times with clear water, dehydration is standby With.
6th, ferment:It is prebiotic using the extract-inulin solution and the fermentation generation of mulberry leaf mixing pile of natural plants witloof Bacterium.To improve function of intestinal canal, promote to digest and assimilate, reduce T-CHOL and triglyceride levels, prevention " three high ", constipation and intestines Cancer.
Inulin is the mixture of a kind of natural levulan, is that levulan unit is formed and by the connection of (2-1) chain with grape The carbohydrate that sugar unit terminates, average degree of polymerization 10-30.It is another energy storage shape of the plant in addition to starch Formula is very good functional food ingredient.It is not hydrolyzed into monose on stomach and intestine top, is directly entered colon as beneficial bacterium Group is reduced gut pH, is stimulated the growth of intestinal mucosa, more had in the absorption to mineral element using multiplication.It is a kind of bifid The proliferation factor of bacillus is promoted intestinal bifidobacteria to be incremented by with 40 times, is proliferated 20% after a week.Daily intestines can be made for 10 grams Road profitable strain increases the production that 44.6% toxic fermentate and 40.9% harmful bacteria enzyme can be reduced within 5-10 times, three weeks Raw, serum cholesterol can reduce 20-50% within three to four months, and blood-fat and blood sugar accordingly reduces, and diastole pressing reduces 799.8P2, metabolic function enhancing improve combination of the colon calcium to protein, promote the absorption of minerals, liver protection beauty subtracts Fertilizer, varieties of food items can be used.On March 25th, 2009, health ministry delivered No. five bulletin, and approval inulin is new resources Food.Specific method is that inulin is configured to the solution that mass percentage is 10-20%, by the mulberry leaf after water-removing and solution Mass ratio is 100:1-2 material compares to be mixed with mulberry leaf, and pile fermentation 2-3h, thickness 18-22cm, temperature control is at 25 DEG C-30 ℃。
7th, shaping is kneaded:By in the mulberry leaf input tea roller drying machine after fermentation, carry out kneading shaping.According to " light- The principle of weight-light " rolls shaping.The method for first using low temperature fast-turn construction, distributes moisture, between temperature is 60 DEG C -70 DEG C, operating 5-6min;The method of mid-term warm fire slow-speed, is allowed to be molded, and temperature is controlled at 78 DEG C -82 DEG C, the duration of runs about 5min or so, Moisture is made to be down to less than 10%;Later stage with high temperature flavouring, sizing, protect color, with 80 DEG C -100 DEG C or so of high temperature, operate 1min's Time makes biodiversity percentage composition be down to 5%-6%, quickly removes, and cooling detects packaging and storage.
The mulberry leaf plucked back chicory extract-inulin zymotic fluid is mixed with mulberry leaf, pile fermentation generation probiotics.Again A kind of pure natural prebiotic tea of GABA mulberry leaf is made with distinctive tea-manufacturing technology.The prebiotic tea of the mulberry leaf is decaffeinated, rich in GABA, For content up to 350mg -480mg/100g, lysine is up to 1233mg/100g, 18 kinds of amino acid and needed by human eight kinds of ammonia Base acid adjustment.Improve function of intestinal canal, Constipation and intestinal cancer promote brain blood flow, and prevention senile dementia and fearness gold are gloomy Family name's disease, enhancing is immune, improves sleep.Prevent " three high " " rich people's disease ".
The positive effect of the present invention is:
Be raw material with high-quality protein mulberry leaf with a kind of biotransformation method, by based on paddy amino acid, using nitrogen, phosphorus, potassium as Auxiliary nutrient solution carries out root and is segmented the method sprayed outside, realizes that biological concentration turns using the photosynthesis and day and night temperature of plant Change, improve the content of GABA.The mulberry leaf plucked back chicory extract-inulin zymotic fluid is mixed with mulberry leaf, pile fermentation generation Probiotics.A kind of pure natural prebiotic tea of GABA mulberry leaf is made with distinctive tea-manufacturing technology again.It is decaffeinated, rich in GABA, make GABA reaches 350mg -480mg/100g, and lysine is up to 1233mg/100g, 18 kinds of amino acid and needed by human eight kinds of amino Sour adjustment.Improve function of intestinal canal, Constipation and intestinal cancer promote brain blood flow, prevention senile dementia and fearness gold Sen Shi Disease, enhancing is immune, improves sleep.Prevent " three high " " rich people's disease ".
Specific embodiment
Below in conjunction with specific example, explanation is explained in detail to the present invention.
Embodiment 1:
The high-quality protein mulberry leaf of plantation, comprise the following steps:
Under conditions of albumen mulberry leaf normally plantation, increase the early period in the growth of albumen mulberry leaf, mid-term and later stage respectively into Row blade face is segmented the step of spray nutritious liquor;The mass percentage of each substance in nutrient solution:Sodium glutamate 3%, urea 0.3%, Calcium superphosphate 0.5%, potassium sulfate 0.3%, plant ash 1.0%, surplus are water, and gross mass percentage is 100%, is claimed according to more than content Raw material is taken, is then prepared as the nutrient solution of 100kg.It is sprayed in the morning of fine day or dusk, sprays 100-150 kilograms per acre Nutrient solution upwards sprays the nozzle of sprayer when spraying, nutrient solution is sprayed on the back side of mulberry leaf;In the leaf growth phase It sprays 3-4 times, is sprayed once every a season.Then the high-quality protein mulberry leaf for being in the albumen mulberry leaf normally picked.
The high-quality protein mulberry leaf 100g rich in GABA is taken, handle is removed, is cut into the small pieces of 2cm × 10cm, teds in dry and ventilated Interior, make moisture evaporation, blade allowed to soften, temperature control is at 30 DEG C or so, airing 3-5h.Then mulberry leaf are inserted 100 DEG C, Mass percentage is used to finish for 0.5% brine in pot, impregnates 30s, with green removing in high temperature, the activity of enzyme in mulberry leaf is destroyed, goes It eliminates the unusual smell, blade is made to soften.It is rinsed again with clean water secondary.By the inulin that the mulberry leaf mass percentage after water-removing is 10% Solution is by material mass ratio 1:100 carry out spraying pile fermentation 2-3h, and temperature is maintained between 25 DEG C -30 DEG C.Then bunching machine is entered Drying and moulding makes moisture be down to less than 7%, detection storage.
Embodiment two:
The plantation of high-quality protein mulberry leaf is planted according to the method in embodiment one, then obtains the high-quality egg of high GABA Bai Sangye.
The high-quality protein mulberry leaf 100g of high GABA is taken, handle is removed, is cut into the fritter of 2cm × 10cm, airing is filled in outdoor solar light The place of foot, thickness 1cm or so, temperature is between 25 DEG C -30 DEG C, airing 4h, is pinched with hand agglomerating, looses one's grip and dissipates for degree, moisture About 60% or so.In 100 DEG C of boiling water of the mulberry leaf input containing 0.5% sodium chloride, 30s is impregnated, it is secondary to take out rinsing.Quality is used again Percentage composition is 0.6% inulin solution, is 1 according to mulberry leaf and inulin solution quality ratio:100 ratio carries out mixing pile hair Ferment 3h.Then carry out kneading shaping with 80 DEG C of temperature, with 100 DEG C of high temperature flavouring 1min, drop the mass percentage of water To less than 8%, cooling, detection, metering, packaging, storage are quickly removed.

Claims (1)

1. a kind of preparation method of the prebiotic tea of high gamma-aminobutyric acid mulberry leaf, it is characterised in that the high gamma-aminobutyric acid mulberry leaf are prebiotic Tea specifically includes following steps using high-quality protein mulberry leaf as raw material:
(1) dedusting:Clean water is carried out spray clean to blade face with sprayer before high-quality mulberry leaf are picked, dust is removed, ensures Sanitation and hygiene;
(2) pick:It is picked immediately after the moisture of mulberry leaf is done;
(3) break and cut:The mulberry leaf of picking are cut into the small pieces of 2cm × 10cm;
(4) airing:Mulberry leaf airing after cutting is in sunny and clean place, and temperature control is at 28 DEG C -32 DEG C, airing 3- 5h;
(5) finish:Mulberry leaf after drying in the air are entered to finish in pot, the saline solution for being 0.3-0.5% with mass percentage, mulberry leaf are with eating The quality of brine is 1:6,30s is impregnated with 100 DEG C of boiling saline solution, is then rinsed 2 times with clear water, dehydration is spare;
(6) pile ferments:Inulin is configured to the solution that mass percentage is 1-2%, the mass ratio by mulberry leaf and solution is 100:1-2 material compares to be mixed with mulberry leaf, and pile fermentation 2-3h, thickness 18-22cm, temperature control is at 25 DEG C-30 DEG C;
(7) shaping is kneaded:It is kneaded with roller machine, temperature is 78 DEG C -82 DEG C, 5min is operated, finally with 100 DEG C of high temperature flavouring 1min makes the mass content of water be down to less than 7%, and quickly removes cooling, is finished product;
The method for planting of the high-quality protein mulberry leaf comprises the following steps:
(1) blade face segmentation spray nutritious liquor is carried out respectively in the early period of albumen mulberry leaf growth, mid-term and later stage;
(2) dosage sprayed:100-150 kilograms of nutrient solution is sprayed per acre;
(3) time sprayed:It is sprayed in the morning of fine day or dusk, the rainy day is unsuitable;
(4) position sprayed:The nozzle of sprayer is sprayed upwards when spraying, nutrient solution is sprayed on the back side of mulberry leaf;
(5) number sprayed:It is sprayed 2-4 times in the leaf growth phase of albumen mulberry leaf;The nutrient solution be based on glutamic acid, Nutrient solution supplemented by nitrogen, phosphorus, potassium, the mass percentage in nutrient solution shared by each substance:Sodium glutamate 2-6%, urea 0.3- 0.5%, calcium superphosphate 0.5-1%, potassium sulfate 0.3-0.5%, plant ash 1.0%, surplus are water, and gross mass percentage composition is 100%.
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