CN105046108B - Corn hybridization compound formulation and system based on self-mating system SSR and phenotypic information - Google Patents
Corn hybridization compound formulation and system based on self-mating system SSR and phenotypic information Download PDFInfo
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Abstract
The present invention discloses a kind of corn hybridization compound formulation and system based on self-mating system SSR and phenotypic information, and method includes：Default self-mating system field test data are standardized；To the self-mating system field test data after standardization, it is determined that presetting the value of feature in each self-mating system, and according to the value of default feature, the selected phenotype distance for hybridizing system is calculated；According to phenotype distance, the defects of determining default self-mating system field test data degree and excellent degree；According to default self-mating system simple repeated sequence SSR molecular marker data, it is determined that in each self-mating system pre-set level value；According to the value of pre-set level, the self-mating system for meeting preparatory condition is defined as hybridization and assembled；Calculate the genetic distance of selected hybridization system；According to phenotype distance and genetic distance, the heterosis, hybrid vigor rate index for being selected in parents is established；Calculate reciprocal cross value SV；According to close heterosis rate MD, the average value of parents' comprehensive valueAnd reciprocal cross value SV, obtain hybridization and assemble value HV.
Description
Technical field
The present invention relates to planting industry informationization technology field, and in particular to a kind of based on self-mating system SSR and phenotypic information
Corn hybridization compound formulation and system.
Background technology
Breeding output is small probability event, and only by large scale investment, small probability event could be converted into inevitable thing
Part, originally accidental breeding output is set to become to be contemplated that, can design.Due to lacking appropriate Method and kit for support, the country educates
Kind of team can not the extensive breeding material of management and control and data message, it is difficult to the breeding rule such as accumulation choosing system and combo, causes experience
Assemble prevailing so that China's corn breeding output has very big uncertainty.Design and context based on molecule is considered as to improve
The important channel of breeding efficiency, but because data, the missing of Method and kit for, domestic Molecular design breeding are substantially at concept
Stage, without exercisable techniqueflow and breeding practice.
In recent years, influence of the development of molecular biotechnology to Crop Genetic Breeding is increasing, including transgenic technology
Quickly grown with the Crops Molecular Breeding of Molecular Marker Assisted Selection Technology.The type of molecular labeling mainly includes：Limitation
Property fragment length polymorphism (Restriction Fragment Length Polymorphism, RELP) mark, random amplification
Polymorphic dna (Random Amplified Polymorphim DNA, RAPD) mark, AFLP
(Amplified Fragment Length Polymorphism, AFLP) mark, simple repeated sequence (Simple
Sequence Repeat, SSR) mark, internal simple repeated sequence (Inter-Simple Sequence Repeat, ISSR)
Mark, SNP (Single Nucleotide Polymorphism, SNP) mark, EST
(Expressed Sequence Tag, EST) mark etc. is a variety of, and what application was most at present is SSR marker.
Traditional cross breeding method has only used DNA fingerprint data, lacks and the necessary of phenotypic data combines.
The content of the invention
The technical problems to be solved by the invention are that traditional cross breeding method has only used DNA fingerprint data, lack and
Necessary the problem of combining of phenotypic data.
For this purpose, in a first aspect, the present invention proposes that a kind of corn hybridization based on self-mating system SSR and phenotypic information assembles
Method, including：
S1, default self-mating system field test data are standardized；
S2, to the self-mating system field test data after standardization, it is determined that preset the value of feature in each self-mating system, and
According to the value of the default feature, the phenotype distance for being selected in self-mating system is calculated；
S3, according to the phenotype distance, the defects of determining the default self-mating system field test data degree and excellent degree；
S4, according to default self-mating system simple repeated sequence SSR molecular marker data, refer to it is determined that being preset in each self-mating system
Target value；
S5, the value according to the pre-set level, the self-mating system for meeting preparatory condition is defined as hybridization and assembled；
S6, the genetic distance for calculating selected hybridization system；
S7, according to the phenotype distance and genetic distance, establish the heterosis, hybrid vigor rate MD of selected parents；
S8, calculate reciprocal cross value SV；
S9, according to the heterosis, hybrid vigor rate MD of the selected parents, the average value of parents' comprehensive valueAnd reciprocal cross
SV is worth, hybridization is obtained and assembles value HV.
Optionally, the step S1, including：Default self-mating system field test data are converted into relative value.
Optionally, in the step S2, the default feature includes：It is yield, seedling, Ear production and grain, disease-resistant, anti-
Worm, anti-fall, breeding time and strain fringe type.
Optionally, in the step S4, the pre-set level, including：Effective number of sites, miscellaneous band number and heterozygosis rate；
Wherein, the effectively number of sites is point value in n site of the default self-mating system SSR molecular marker data
The number in the site being not zero, n are preset constant；
Wherein, the miscellaneous band number is different loci value in n site of the default self-mating system SSR molecular marker data
Number；
Wherein, the calculation formula of the heterozygosis rate is as follows：
Heterozygosis rate=miscellaneous band number/n.
Optionally, in the step S5, the preparatory condition for self-mating system homozygosity be more than the first predetermined threshold value and
Effective number of sites is more than the second predetermined threshold value.
Second aspect, the present invention also propose that a kind of corn hybridization based on self-mating system SSR and phenotypic information assembles system, wrap
Include：
Processing unit, for being standardized to default self-mating system field test data；
Computing unit, for the self-mating system field test data after standardization, it is determined that being preset in each self-mating system
The value of feature, and according to the value of the default feature, calculate the phenotype distance for being selected in self-mating system；
Determining unit, the defects of for according to the phenotype distance, determining the default self-mating system field test data
Degree and excellent degree；
The determining unit, it is additionally operable to according to default self-mating system simple repeated sequence SSR molecular marker data, it is determined that often
The value of pre-set level in individual self-mating system；
The determining unit, the value according to the pre-set level is additionally operable to, the self-mating system for meeting preparatory condition is defined as
Hybridization assembles；
The computing unit, it is additionally operable to calculate the genetic distance of selected hybridization system；
Unit is established, for according to the phenotype distance and genetic distance, establishing the heterosis, hybrid vigor rate MD for being selected in parents；
The computing unit, it is additionally operable to calculate reciprocal cross value SV；
The determining unit, it is additionally operable to heterosis, hybrid vigor rate MD according to the selected parents, parents' comprehensive value is averaged
ValueAnd reciprocal cross value SV, obtain hybridization and assemble value HV.
Compared to prior art, corn hybridization compound formulation of the invention based on self-mating system SSR and phenotypic information and it is
System, integrated management corn strain molecular labeling and field phenotype test data, overall merit corn inbred line, assisting sifting, point
The hybrid vigour based on self-mating system molecular labeling and phenotypic difference and simulation apolegamy Maize hybridized combination, performance prediction are analysed, is
Improve China's corn breeding scale and information regulatory level provides Method and kit for and supported.
Brief description of the drawings
Fig. 1 shows that a kind of corn hybridization based on self-mating system SSR and phenotypic information assembles disclosed in the embodiment of the present invention
Method flow diagram；
Fig. 2 shows that field test disclosed in the embodiment of the present invention tests definition figure；
Fig. 3 shows DNA fingerprint detection experiment definition figure disclosed in the embodiment of the present invention；
Fig. 4 shows the disclosed self-mating system title figure for repeating detection of the embodiment of the present invention；
Fig. 5, which is shown, checks fingerprint homogeneity system schematic diagram disclosed in the embodiment of the present invention；
Fig. 6 shows parent's preliminary screening condition schematic diagram disclosed in the embodiment of the present invention；
Fig. 7 shows parent's Integrated Selection schematic diagram disclosed in the embodiment of the present invention；
Fig. 8 shows the seed selection schematic diagram of the anti-withered kind of green grass or young crops disclosed in the embodiment of the present invention；
Fig. 9 is shown to be illustrated after self-mating system assembles disclosed in the embodiment of the present invention according to the sequence of cross combination comprehensive value
Figure；
Figure 10 shows the screening schematic diagram of cross combination disclosed in the embodiment of the present invention.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is explicitly described, it is clear that described embodiment be the present invention
Part of the embodiment, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not having
The every other embodiment obtained under the premise of creative work is made, belongs to the scope of protection of the invention.
The present embodiment discloses a kind of corn hybridization compound formulation based on self-mating system SSR and phenotypic information, including：
S1, default self-mating system field test data are standardized；
S2, to the self-mating system field test data after standardization, it is determined that preset the value of feature in each self-mating system, and
According to the value of the default feature, the phenotype distance for being selected in self-mating system is calculated；
" selected self-mating system "：In terms of two, one, be standardized based on self-mating system phenotype test data after, calculate
The indexs such as parent's value for being respectively, yield resistant properties, excellent degree, degree of imperfection, for Evaluating and screening self-mating system；Based on self-mating system
SSR molecular marker data, it is the indexs such as effective number of sites, heterozygosity to calculate each, for Evaluating and screening self-mating system.(Phenotype Number
According to gene aspect) "
Calculating phenotype distance "：Calculation formula isWherein, PDijIt is ith and jth selfing
The phenotypic genetic distance of system, VikAnd VjkIt is the value after k-th of criterion of ith and jth self-mating system respectively, wkIt is k-th
The weight of index, n are efficiency index numbers.
S3, according to the phenotype distance, the defects of determining the default self-mating system field test data degree and excellent degree；
Degree of imperfection and excellent degree be to all Field observation indexs of self-mating system (or cross combination) performance " fine ",
" good ", " poor ", the statistic of " very poor ".The statistic is the index degree reached to field test data, successively " fine ",
" good ", " poor ", " very poor " are according to 3:2:2:The 3 attached weighted value of ratio is weighted obtained by average computation.And " fine " standard is
If referring to average value of value of the cross combination in certain index more than all combinations in the index adds 2 times of standard deviations, recognize
Shown as " fine " in this index for the combination.If the value in certain index is less than all cross combinations in the index
Average value subtracts 2 times of standard deviations, then it is assumed that the combination is shown as " very poor " in this index.Wherein, " fine ", " good ",
The standard deviation multiple of " poor ", " very poor " can be configured by software.By the production of diversity factor and degree of imperfection combination self-mating system
The features such as amount resistance and comprehensive value are used to screen self-mating system.
S4, according to default self-mating system simple repeated sequence SSR molecular marker data, refer to it is determined that being preset in each self-mating system
Target value；
S5, the value according to the pre-set level, the self-mating system for meeting preparatory condition is defined as hybridization and assembled；
S6, the genetic distance for calculating selected hybridization system；
The calculation formula of " genetic distance " is as follows：
Wherein NijThe band number shared for two kinds (being), NiAnd NjRepresent that ith and jth kind (being) is respective respectively
Band number.
S7, according to the phenotype distance and genetic distance, establish the heterosis, hybrid vigor rate MD of selected parents；
Data and index based on selection self-mating system calculate phenotype distance and genetic distance, with reference to phenotype distance and heredity away from
From the heterosis, hybrid vigor rate index for establishing selected parents so as to selecting parents.
S8, calculate reciprocal cross value SV；
Orthogonal value calculation formula is Vij=HD+r1*(ti-tj)+r2*(hi-hj) wherein, HD is the synthesis valency of self-mating system
Value, VijIt is the orthogonal value of ith and jth parent, tiIt is the standardized value of the spinning phase of i-th parent, tjIt is j-th of parent
Loose powder phase standardized value, hiIt is the plant height of i-th of parent, hjIt is the Ear height of j-th of parent, r1And r2Respectively represent spinning-
(such as male parent plant height is more than maternal fringe position for just to the weights of importance and directivity factor of loose powder interval and plant height-fringe potential difference
To).Take being calculated as follows for reciprocal cross value：
SV=Max (Vij,Vji)。
S9, according to the heterosis, hybrid vigor rate MD of the selected parents, the average value of parents' comprehensive valueAnd reciprocal cross
SV is worth, hybridization is obtained and assembles value HV.
Parents' comprehensive value average valueIt is calculated as (male parent comprehensive value+maternal comprehensive value)/2, comprehensive value HD
It is calculated as：
vkIt is the standardized value of k-th of Field observation index, wkIt is the weight of k-th of Field observation index.Two with reference to more than
Person and reciprocal cross value SV, obtain hybridization and assemble value HV.Cross combination value (HV) is for describing cross combination exploitation
The overall target of value, cross combination is costly is assembled for selection.
In a specific example, the step S1, including：Default self-mating system field test data are converted into phase
To value.
It is converted into the meaning of relative value：Absolute value data or relative value number are generally used in the performance of self-mating system under various circumstances
According to describing, absolute value data is typically got by the direct observation to index, its used dimension, be and specific observation
Unrelated independent reference system.If the data of record do not indicate data unit, absolute value data becomes meaningless.Relative value
Data use data to be recorded in the order in totality or the statistic relevant with totality as reference, its informative, are easy to
Understand, be the evaluation method mainly used in inbred line evaluations.
In a specific example, in the step S2, the default feature includes：Yield, seedling, Cheng Suijie
Real, disease-resistant, pest-resistant, anti-fall, breeding time and strain fringe type.
In a specific example, in the step S4, the pre-set level, including：Effective number of sites, miscellaneous band number
With heterozygosis rate；
Wherein, the effectively number of sites is point value in n site of the default self-mating system SSR molecular marker data
The number in the site being not zero；N typically selects 40 according to the requirement of SSR fingerprint detections；
Wherein, the miscellaneous band number is different loci value in n site of the default self-mating system SSR molecular marker data
Number；
Wherein, the calculation formula of the heterozygosis rate is as follows：
Heterozygosis rate=miscellaneous band number/n.
In a specific example, in the step S5, the preparatory condition is more than for the homozygosity of self-mating system
One predetermined threshold value and effective number of sites is more than the second predetermined threshold value.
Wherein, the homozygosity of self-mating system=homozygote site number/effective site number.
The present embodiment discloses a kind of corn hybridization based on self-mating system SSR and phenotypic information and assembles system, including：
Processing unit, for being standardized to default self-mating system field test data；
Computing unit, for the self-mating system field test data after standardization, it is determined that being preset in each self-mating system
The value of feature, and according to the value of the default feature, calculate the phenotype distance for being selected in self-mating system；
Determining unit, the defects of for according to the phenotype distance, determining the default self-mating system field test data
Degree and excellent degree；
The determining unit, it is additionally operable to according to default self-mating system simple repeated sequence SSR molecular marker data, it is determined that often
The value of pre-set level in individual self-mating system；
The determining unit, the value according to the pre-set level is additionally operable to, the self-mating system for meeting preparatory condition is defined as
Hybridization assembles；
The computing unit, it is additionally operable to calculate the genetic distance of selected hybridization system；
Unit is established, for according to the phenotype distance and genetic distance, establishing the heterosis, hybrid vigor rate MD for being selected in parents；
The computing unit, it is additionally operable to calculate reciprocal cross value SV；
The determining unit, it is additionally operable to heterosis, hybrid vigor rate MD according to the selected parents, parents' comprehensive value is averaged
ValueAnd reciprocal cross value SV, obtain hybridization and assemble value HV.
System in the present embodiment can perform the step in above method embodiment, and the present embodiment is no longer described in detail.
As shown in figure 1, the idiographic flow following 4.1 to 4.8 of above-described embodiment：
4.1 are standardized to self-mating system phenotype test data first：At self-mating system field test data normalization
Reason refers to the processing procedure that the field data using pilot average as reference system converts to relative value.Liu Zhe etc. is proposed with class mean
For 3S the and 2S conversion values of reference system, normal bit sub-value, using class mean as 5 kinds of variety test data such as the class NDVI values of reference system
Relative value method for transformation, and the changing effect of 5 kinds of methods is analyzed with " standard deviation ratio ", analysis result shows with group
Average for reference system 3S and 2S conversion values closer to kind hereditary feature.This method used herein identifies number to self-mating system
Value type data in carry out relative value conversion.
4.2 based on the self-mating system field test data after standardization, calculate the yield of each self-mating system, seedling, into
Feature and its comprehensive values such as fringe is solid, economic value, disease-resistant, pest-resistant, anti-fall, breeding time and strain fringe type, and for calculate into
Select the phenotype distance of self-mating system.The comprehensive value of self-mating system is the synthesis item of Field observation data.
4.3 be evaluation self-mating system and the comprehensive advantage and inferior position of cross combination, on the basis of the phenotypic character of existing observation,
Devise degree of imperfection and excellent degree two indices.Degree of imperfection and excellent degree are all fields sights to self-mating system (or cross combination)
Survey index performance " fine ", " good ", " poor ", the statistic of " very poor ".The statistic is the index reached to field test data
Degree, " fine ", " good ", " poor ", " very poor " are according to 3 successively:2:2:The 3 attached weighted value of ratio is weighted average computation institute
.
The processing of 4.4 self-mating system SSR data：Based on self-mating system SSR molecular marker data, the effective of each self-mating system is calculated
The indexs such as number of sites, miscellaneous band number and heterozygosis rate.Effective number of sites refers to n site of selected SSR data, in this n site
The site for having value is exactly effective site；The self-mating system of general measure SSR data is all homozygous, i.e., should be on each site
Identical value, but homozygosis is actually not necessarily, miscellaneous band number refers to how many different value on this n site, and miscellaneous band number is just
It is how many；Heterozygosis rate refers to quantity/n of heterozygosis band.These three indexs are used for the homozygosity for judging self-mating system, screen homozygosity
Self-mating system more than high, effective number of sites assembles into hybridization, and calculates the genetic distance of selected self-mating system.
4.5 combine the heterosis, hybrid vigor rate index of phenotype distance and the selected parents of genetic distance foundation：Utilize phenotypic data meter
The phenotypic difference that phenotypic genetic distance represents hybrid parents is calculated, calculating molecular labeling genetic distance with DNA fingerprint data is expressed as
The genotypic difference of parents, both are not exclusively overlapping each other, describe the hereditary difference of parents from different perspectives.The present invention is with one
Close heterosis rate (MD) summarizes the hereditary difference of parents in individual, be designated as phenotypic genetic distance and SSR molecular marker heredity away from
From linear equation.
4.5.1 the calculating of phenotypic genetic distance：For number of computations character and the phenotypic genetic distance of qualitative character, and just
Realized in software programming, with reference to data normalization of the present invention processing, using mode is calculated as below：
4.5.2 molecular labeling genetic distance algorithm：Genetic distance is that some character complex inheritance differences are big between weighing kind
Small index.Character more than one required by breeding objective, in order to more fully reflect that the heredity between parental breed is poor
It is different, it is necessary to consider to multiple characters, so as to amplify out the concept of genetic distance.Kind is calculated by Multielement statistical analysis method
Between the geometric distance of hyperspace that forms of multiple character gene offsets, be just called interracial genetic distance.
The calculating of 4.6 reciprocal crosss value：The main degree of cooperation for considering Parent specialized character of reciprocal cross value, at these
In character, Parent is not closer better, but to have different, could be hybrid seeding or play hybrid vigour
To bigger effect, such specialized character such as Parent plant height-fringe potential difference, spinning-loose powder interval etc..Counted respectively in the present invention
Orthogonal value and reciprocal cross value are calculated, and with the greater between the two, the reciprocal cross as cross combination is worth.
4.7 cross combinations value (HV) is the overall target for describing cross combination Development volue, parent in using herein
Heterosis rate (MD), the average value of parents' comprehensive valueAnd reciprocal cross is worth (SV) to represent cross combination valency
Value.
4.8 by the field data after standardization and DNA data import system databases, then the present invention " be based on from
Friendship is that the corn hybridization of SSR and field test assembles systematical design idea " in complete.
Below with an instantiation, illustrate the course of work of the invention, including 5.1 to 5.9：
5.1st, default self-mating system field test data and DNA fingerprint data are：2009 Xunxian, Gongzhuling, Tieling,
Six Dandong, Shunyi, Zhumadian pilots amount to 482 parts of self-mating system field test materials, are examined by SSR molecular marker DNA fingerprint
The self-mating system of survey amounts to 570 parts, 179 parts altogether of both coincidences, corn inbred line field test standard data format such as table one,
Maize dna fingerprint standard data format such as table two：
The corn inbred line field test standard data format of table one
| Pilot title | Cell number | Sowing date | Emerge the date | Emergence sum | Without seedling cave number | Seedling gesture is strong and weak |
| Gongzhuling | CZ10001 | 2010/5/7 | 2010/5/21 | 43 | 3 | 0 |
| Gongzhuling | CZ10002 | 2010/5/7 | 2010/5/21 | 37 | 9 | 0 |
| Gongzhuling | CZ10003 | 2010/5/7 | 2010/5/21 | 45 | 1 | 0 |
The maize dna fingerprint standard data format of table two
| Self-mating system title | Field line number | Application type | nP1 | nP3 | nP4 | nP5 | nP6 |
| CZ09147 | 6265 | It is female | a | a | a | a | a |
| CZ09154 | 6274 | Father | a | a | a | a | a |
| CZ09548 | 6316 | Father | a | a | a | a | b |
| CZ09041 | 6071 | It is female | a | b | b | a | b |
5.2 addition experimental groups
Addition test group will define field test experiment respectively and DNA fingerprint detects the essential information of experiment.Field test
Experiment defines the essential informations such as the time for defining test group in systems, density, cell conditions, and system is that the experiment determines one
Individual unique ID, this ID are associated for lists such as the indexs selected by the storage with experimental group number evidence, test group, therefore try
It is very necessary to test a group definition.Self-mating system field test data in the present invention due to 2009 only have a density, therefore press
Two test groups are divided into according to spring sowing/summer sowing, field test experiment definition is as shown in Fig. 2 DNA fingerprint detection experiment is defined as schemed
Shown in 3.
5.3 field test data loadings
The data that field collection comes, according to the great Qu where experiment, spring sowing/summer sowing, density group division test group, and
Collected in Excel by test group.Respectively in Excel each test group data carry out data check, including self-mating system,
Whether pilot, the title of index the null value inspection of data, repeat row inspection etc. with having the inspection of title duplication of name in database.
5.4 before DNA fingerprint data are submitted to database, it is also necessary to whether have repetition detection selfing to the data of submission
System is checked that the detection function provided by system, it is to repeat to detect that can quickly view which self-mating system.If no
This operation is carried out, system default retains the data that the self-mating system occurs for the first time.Oneself of repetition detection is shown on the right side of Fig. 4
Friendship is title.
5.5 pairs of miscellaneous bands are excessive or effective detection site detection sample very little will carry out secondary detection, because used is drawn
Thing is the same, and the result of repeated detection can be defined in a DNA detection experiment.If a self-mating system has a plurality of detection
Record, can only retain most rational one.For heterozygous sites, be both probably sample it is impure caused by result, it is also possible to quilt
Sample material is also not up to homozygous in itself.
5.6 corn characteristic values calculate：Data normalization process, the calculating collected comprising self-mating system multiple years.Use herein
Family can be customized to the N-S method standard deviations multiple of each index, overflow value bound parameter.
After being standardized to field data, also need the self-mating system DNA fingerprint progress heredity to typing similar
The fingerprint characteristics such as coefficient matrix calculates, heterozygosis rate statistics, " fingerprint homogeneity system " detection calculate, according to result of calculation to breeding parent
Preliminary screening is carried out, excludes the too high self-mating system of heterozygosis rate.As shown in figure 5, " checking fingerprint homogeneity system " column represents to be red
" fingerprint homogeneity system " be present in current detection in the self-mating system.
" fingerprint homogeneity system " detection is by inquiring about self-mating system genetic similarity matrix, is 1 genetic similarity
The select process of self-mating system.
5.7 parent's preliminary screenings
Parental line selection is divided into two links of preliminary screening and Integrated Selection in text, can be entered after fingerprint characteristic calculating
Row preliminary screening：According to heterozygosis rate and " fingerprint homogeneity system " information, to excluding, heterozygosis rate is too high or title is different but substantial
For the data record of same self-mating system.Present example is according to heterozygosis rate>=0.1 or effective number of sites<=18 or miscellaneous band numbers>=2
Condition screened, as shown in Figure 6.
94 parts of selfing based materials are excluded by this condition, table three show the self-mating system of part exclusion and excludes reason：
The self-mating system and exclude reason that table three parts exclude
In " fingerprint homogeneity system " the partial detection such as table four of the self-mating system to 570 parts of participation DNA fingerprints：
Table four " fingerprint homogeneity system " partial detection
5.8 parent's Integrated Selections：The field index weights set using upper one section, and the evaluation self-mating system of acquiescence are respectively referred to
Mark horizontal " very poor ", " poor ", " preferable ", the standard deviation multiple " -2 " of " good ", " -1 ", " 1 ", " 2 ", self-mating system according to comprehensive valency
Value is as shown in Figure 7.
The seed selection of the anti-withered kind of green grass or young crops needs parents are withered to green grass or young crops all to have certain resistance, while requires that yield level can not be too again
It is low.Therefore following screening conditions are designed, as shown in Figure 8.
5.9 cross combinations are screened
, it is necessary to be configured to each parameter of hybrid vigour, row-column value, combination value before cross combination preparation.
With necessarily empirical, different people has differences to the understanding of the importance of each factor, directly results in miscellaneous for the setting of parameter
Hand over the difference of combination evaluation result.In hybrid vigour parameter setting, due to self-mating system field test used in example containing only
The data of 2009 one years, testing site are time less, and its reliability does not have that DNA fingerprint data are high, thus assign molecular labeling heredity away from
From higher weight.In the parameter setting of reciprocal cross (row-column, column-row) value, it is considered herein that Parent discrepancy in elevation ratio is told
Silk-loose powder interval is more important, therefore assigns 0.05 and 0.1 weight respectively to row-column florescence, the row-column discrepancy in elevation.In combination value
In parameter setting, according to parental genetic difference, parental mean performance, the principle that weakens successively of florescence discrepancy in elevation harmony importance,
This three parts are assigned with 1,0.5,0.2 weight respectively.
Fig. 9 enters to be assembled two-by-two to 78 parts of self-mating systems according to cross combination comprehensive value to 3003 cross combinations
The interface of row sequence.
Screening to cross combination is carried out according to condition as shown in Figure 10.
The cross combination for meeting this condition is excluded, and finally obtains 37 parts of cross combinations (partial results), as shown in Table 5.
Partial hybridization after table five screens combines
Compared to prior art, corn hybridization of the invention assembles system integration management corn strain molecular labeling and field
Phenotype test data, overall merit corn inbred line, assisting sifting, analyze miscellaneous based on self-mating system molecular labeling and phenotypic difference
Kind advantage and simulation apolegamy Maize hybridized combination, performance prediction, for raising China's corn breeding scale and information regulatory level
Method and kit for is provided to support.
It should be noted that herein, first predetermined threshold value and the second predetermined threshold value are used merely to a threshold
Value and another threshold value make a distinction, rather than imply the relation or order between the two threshold values.
In addition, it will be appreciated by those of skill in the art that although some embodiments described herein include other embodiments
In included some features rather than further feature, but the combination of the feature of different embodiments means in of the invention
Within the scope of and form different embodiments.
Although being described in conjunction with the accompanying embodiments of the present invention, those skilled in the art can not depart from this hair
Various modifications and variations are made in the case of bright spirit and scope,
Such modifications and variations are each fallen within be defined by the appended claims within the scope of.
Claims (6)
1. the corn hybridization compound formulation based on self-mating system SSR and phenotypic information, it is characterised in that including：
S1, default self-mating system field test data are standardized；
S2, to the self-mating system field test data after standardization, it is determined that preset the value of feature in each self-mating system, and according to
The value of the default feature, calculate the phenotype distance of selected self-mating system；
S3, according to the phenotype distance, the defects of determining the default self-mating system field test data degree and excellent degree；
S4, according to default self-mating system simple repeated sequence SSR molecular marker data, it is determined that pre-set level in each self-mating system
Value；
S5, the value according to the pre-set level, the self-mating system for meeting preparatory condition is defined as hybridization and assembled；
S6, the genetic distance for calculating selected hybridization system；
S7, according to the phenotype distance and genetic distance, establish the heterosis, hybrid vigor rate MD of selected parents；
S8, calculate reciprocal cross value SV；
Orthogonal value calculation formula is Vij=HD+r1*(ti-tj)+r2*(hi-hj)；
Wherein, HD be self-mating system comprehensive value, VijIt is the orthogonal value of ith and jth parent, tiIt is telling for i-th parent
The standardized value of silk phase, tjIt is the loose powder phase standardized value of j-th of parent, hiIt is the plant height of i-th of parent, hjIt is j-th of parent
Ear height, r1And r2The weights of importance and directivity factor of spinning-loose powder interval and plant height-fringe potential difference are represented respectively；
Take being calculated as follows for reciprocal cross value：
SV=Max (Vij,Vji)；
S9, according to the heterosis, hybrid vigor rate MD of the selected parents, the average value of parents' comprehensive valueAnd reciprocal cross value
SV, obtain hybridization and assemble value HV.
2. according to the method for claim 1, it is characterised in that the step S1, including：Default self-mating system field is reflected
Fixed number evidence is converted into relative value.
3. according to the method for claim 1, it is characterised in that in the step S2, the default feature includes：Production
Amount, seedling, Ear production and grain, disease-resistant, pest-resistant, anti-fall, breeding time and strain fringe type.
4. according to the method for claim 1, it is characterised in that in the step S4, the pre-set level, including：Have
Imitate number of sites, miscellaneous band number and heterozygosis rate；
Wherein, it is described effectively number of sites be the default self-mating system SSR molecular marker data n site in point value be not
The number in zero site, n are preset constant；
Wherein, the miscellaneous band number in n site of the default self-mating system SSR molecular marker data different loci value it is individual
Number；
Wherein, the calculation formula of the heterozygosis rate is as follows：
Heterozygosis rate=miscellaneous band number/n.
5. according to the method for claim 4, it is characterised in that in the step S5, the preparatory condition is self-mating system
Homozygosity be more than the first predetermined threshold value and effective number of sites and be more than the second predetermined threshold value.
6. the corn hybridization based on self-mating system SSR and phenotypic information assembles system, it is characterised in that including：
Processing unit, for being standardized to default self-mating system field test data；
Computing unit, for the self-mating system field test data after standardization, it is determined that presetting feature in each self-mating system
Value, and according to the value of the default feature, calculate the phenotype distance of selected self-mating system；
Determining unit, the defects of for according to the phenotype distance, determining the default self-mating system field test data degree and
Excellent degree；
The determining unit, it is additionally operable to according to default self-mating system simple repeated sequence SSR molecular marker data, it is determined that each certainly
Hand over the value of pre-set level in system；
The determining unit, the value according to the pre-set level is additionally operable to, the self-mating system for meeting preparatory condition is defined as hybridizing
Assemble；
The computing unit, it is additionally operable to calculate the genetic distance of selected hybridization system；
Unit is established, for according to the phenotype distance and genetic distance, establishing the heterosis, hybrid vigor rate MD for being selected in parents；
The computing unit, it is additionally operable to calculate reciprocal cross value SV；
Orthogonal value calculation formula is Vij=HD+r1*(ti-tj)+r2*(hi-hj)；
Wherein, HD be self-mating system comprehensive value, VijIt is the orthogonal value of ith and jth parent, tiIt is telling for i-th parent
The standardized value of silk phase, tjIt is the loose powder phase standardized value of j-th of parent, hiIt is the plant height of i-th of parent, hjIt is j-th of parent
Ear height, r1And r2The weights of importance and directivity factor of spinning-loose powder interval and plant height-fringe potential difference are represented respectively；
Take being calculated as follows for reciprocal cross value：
SV=Max (Vij,Vji)；
The determining unit, it is additionally operable to heterosis, hybrid vigor rate MD, the average value of parents' comprehensive value according to the selected parents
And reciprocal cross value SV, obtain hybridization and assemble value HV.
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