CN105043984A - Substance detection kit, optical detection system and detection method - Google Patents

Abstract

The invention provides a substance detection kit, an optical detection system and a detection method. The kit has a simple structure, a reagent can be conveniently fixed in the disk-shaped kit, so operating steps required by user detection are simplified, and the kit can adapt to detection of different substances in various situations; the optical detection system adopts a small rotating kit, and control of a rotating angle variable is only needed in the detection process, so the complexity of the system is simplified, and the stability of the system is increased; three light sources with respectively independently-adjustable color channels are used, so the system can detect different substances through setting of different parameters of the three color light sources and through feedback adjustment; and calculation of the detection accuracy in the detection process of the concentration of a same kind of substances in different samples through two different detection methods enhances the credibility of detection results.

Description

A kind of kit, Systems for optical inspection and detection method detected for material

Technical field

The present invention relates to detection field, be specifically related to a kind of kit, Systems for optical inspection and the detection method that detect for material.

Background technology

In recent years, the optical detection for material is widely used in biology, chemical industry, the industries such as quality inspection.Traditional optical detection means generally can be divided into absorption detecting to detect with transmitting.Launch and detect, namely make use of the character such as the fluorescence that a certain class material has, measure its concentration value by the light measuring electromagnetic radiation.Absorption detecting, namely isolates a certain monochromatic light exposure unknown sample, detects sample to the absorptivity of light and learns the concentration of sample.

The limitation of above detection mode and detection system is:

1, general sample has absorption behavior for the light of each wavelength, adopts the optical properties of narrow wavelength light to a certain narrow bandwidth of sample to measure, reduces the sensitivity of detection, therefore needs to adopt very sensitive sensor, improves testing cost.

2, current checkout equipment Attribute Oriented and not for material, the analysis making user still need user to carry out bringing into typical curve upon completion of the assays can determine material concentration.

3, the compact optical checkout equipment automaticity of traditional low-cost is lower, increases the operating difficulties of user.And how bulky the checkout equipment of high cost is, cause the difficulty of user's Site Detection.

4, the form of the bottled reagent of the many employings of conventional reagents box+square check-out console, this kit form is applicable to the detection method adopting the main equipments such as microplate reader, and is not suitable for the pick-up unit of miniaturization.

5, method many employings computer serial port that conventional detection devices exports data to other equipment exports or wired output form such as USB output, and this output interface is adapted at the analysis of fixed-site, but can not realize user oriented Site Detection.

Summary of the invention

In view of this, the invention provides a kind of kit, Systems for optical inspection and the detection method that detect for material, the applying in a flexible way property of Systems for optical inspection can be increased, simplify the operation steps that material detects, and adopt the integration system of optics, robotization and wireless communication module, make user that other mobile terminals can be used to carry out control and personalized adjustment to each attribute detected and parameter, and the detection of many flux can be carried out at non-laboratory situations to sample.

The technical solution adopted for the present invention to solve the technical problems is:

A kind of kit detected for material of the present invention, this kit is four layers of disc-shaped structure, comprises the top layer, channel layer, functional layer and the bottom that connect in turn from top to bottom;

Wherein, around the center of circle of described channel layer, be circumferentially evenly processed with circular hole, as verification site (4-3); The periphery of verification site (4-3) is circumferentially evenly processed with and the fluid passage (4-6) verifying site (4-3) equivalent; The agent structure of described fluid passage (4-6) is circular hole, and the edge of circular hole has 2 along same diametric strip channel groove; And the center of circle in the center of circle of each fluid passage (4-6) circular hole and corresponding verification site (4-3) is on the same radius of channel layer;

Described top layer is processed with paired detection thing entrance (4-7), its logarithm is consistent with the number of fluid passage (4-6), and often pair of two circular hole positions detecting thing entrance (4-7) are corresponding with the two ends of the strip channel groove of fluid passage (4-6) respectively;

Described functional layer is processed with the circular hole corresponding with the agent structure of the fluid passage (4-6) on channel layer, as detection site (4-8); Functional layer is also processed with the verification site (4-3) corresponding with channel layer.

Preferably, the mode of gummed or thermocompression bonding is adopted to connect between four layers of described kit.

Based on a Systems for optical inspection for described kit, comprise Optical system module (1), kit (4), stepper motor (7), bottom circuit board (8) and user interactive module (9);

Described bottom circuit board (8) comprises detecting sensor (10), check sensor (11) and message processing module;

Described stepper motor (7) drives the center of circle of kit (4) to rotate;

Described Optical system module (1) produces two identical light, and a conduct detects light, and another is as verification light; Detecting light is irradiated in detecting sensor (10) by one of them detection site (4-8), and detecting sensor (10) receives and detects light and after obtaining light intensity data I, be transferred to message processing module; Verification light is irradiated on check sensor (11) by the verification site (4-3) of kit, and check sensor (11) receives verification light and after obtaining light intensity data II, is transferred to message processing module;

Message processing module obtains testing result after comprehensively analyzing the light intensity data I of each detection site (4-8) and the light intensity data II of each verification site (4-3), and testing result is sent to user interactive module (9).

Preferably, described Systems for optical inspection also comprises detection platform (5) and disk-shaped support (6);

The radius of a circle that the verification site (4-3) that the radius of described disk-shaped support (6) is less than described kit (4) is formed; The circle centre position of described detection platform (5) is processed with center hole; Detection platform (5) be also processed with the detection through hole corresponding with detecting sensor (10) and check sensor (11) and verify through hole;

Described detection platform (5) is fixedly mounted on above stepper motor; Described disk-shaped support (6) is placed in detection platform (5); Disk-shaped support (6) is connected with disk-shaped support (6) by the center hole of detection platform (5) with the output shaft of stepper motor (7); Described kit (4) is fixed on disk-shaped support (6); The output shaft of stepper motor (7) drives disk-shaped support (6) to rotate, thus drives kit (4) to rotate.

Preferably, the channel layer of described kit (4) is also processed with magnet pilot hole (4-2) around the center of circle; And functional layer is also processed with the magnet pilot hole (4-2) corresponding with channel layer; Magnet pilot hole (4-2) built with magnet, kit (4) by magnet adsorption on disk-shaped support (6).

Preferably, on the channel layer of described kit (4), the periphery in one of them verification site is processed with laser positioning hole at zero point (4-4), and the center of circle of the center of circle in the center of circle in this laser positioning hole at zero point (4-4), corresponding verification site (4-3) and channel layer at same diametrically; The laser positioning hole (4-5) with fluid passage (4-6) equivalent is evenly processed with in fluid passage (4-6) periphery; And the center of circle of each laser positioning hole (4-5), the center of circle of fluid passage (4-6) circular hole, verification site (4-3) the center of circle and kit center pit (4-1) the center of circle kit same diametrically; In addition, on described bottom, the laser positioning zero point hole (4-4) corresponding with channel layer and laser positioning hole (4-5) is processed with;

Described Systems for optical inspection (1) also comprises laser instrument I (2), laser instrument II (3), photoresistance I (12) and photoresistance II (13);

Photoresistance I (12) and photoresistance II (13) are arranged on described bottom circuit board (8); Detection platform (5) is upper also has the zero point through hole (14) corresponding with photoresistance I (12) and photoresistance II (13) and positioning through hole (15);

Laser instrument I (2) is by the laser positioning hole at zero point (4-4) of kit and be irradiated to through hole at zero point (14) in photoresistance I (12), photoresistance I (12) receives laser, and laser intensity data I are sent to message processing module;

Laser instrument II (3) is irradiated in photoresistance II (13) by the laser positioning hole (4-5) of kit and positioning through hole (15), photoresistance II (13) receives laser, and intensity data II is sent to message processing module;

Analysis result is sent to user interactive module (9) after analyzing laser intensity data I and intensity data II by message processing module; According to analysis result, user interactive module (9) judges whether kit position skew occurs, the position of timely calibration reagent box when there is position skew.

Preferably, described Optical system module (1) comprises light source R (6-1), light source G (6-2), light source B (6-3), light splitting piece I (6-4), light splitting piece II (6-5), light splitting piece III (6-6), light splitting piece IV (6-7), light splitting piece V (6-8), collimation lens (6-9), photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12);

Wherein, light splitting piece I (6-4), light splitting piece II (6-5), light splitting piece III (6-6), collimation lens (6-9), light splitting piece IV (6-7) and light splitting piece V (6-8) are coaxially placed successively; Photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12) are arranged on bottom circuit board (8);

The light that light source R (6-1) sends, be irradiated to light splitting piece I (6-4) and produce reflection and transmission two light afterwards, transmitted ray is irradiated to photodiode I (6-10), the intensity of photodiode I probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source R (6-1); Reflection ray is irradiated on collimation lens (6-9) through light splitting piece II (6-5) and light splitting piece III (6-6) successively;

The light that light source G (6-2) sends, be irradiated to light splitting piece II (6-5) and produce reflection and transmission two light, transmitted ray is irradiated to photodiode II (6-11), the intensity of photodiode II probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source G (6-2); Reflection ray is irradiated on collimation lens (6-9) through light splitting piece III (6-6);

The light that light source B (6-3) sends, be irradiated to light splitting piece III (6-6) and produce reflection and transmission two light, transmitted ray is irradiated to photodiode III (6-12), the intensity of photodiode III probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source G (6-3); Reflection ray is irradiated on collimation lens (6-9);

Three reflection rays that described collimation lens (6-9) will be irradiated on collimation lens (6-9) pool a light, this light produces through light splitting piece III (6-7) reflection and detects light, after light splitting piece III (6-7) transmission, produce verification light again through light splitting piece IV (6-8) reflection.

Preferably, carry out data between message processing module and user interactive module (9) when transmitting, adopt the communication based on agreement to carry out.

Based on a detection method for described Systems for optical inspection, this detection method, for the detection of allied substances concentration in different sample, comprises the steps:

Step 101, injection reagent:

Any solution is not injected in the verification site (4-3) of kit (4); Detection site (4-8) with laser positioning hole at zero point (4-4) same diametrically far-end is labeled as CID=0; A detection site (4-8) adjacent with the detection site (4-8) of CID=0 is labeled as CID=1, all the other detection site (4-8) are labeled as CID=2 in order successively, 3 ..., N-1, N are the sum of detection site (4-8); The each verification site (4-3) corresponding with each detection site (4-8) is labeled as YID=0,1 ..., N-1;

Placebo solution is injected in the detection site (4-8) of CID=0; The contrast solution of concentration known is injected in the detection site (4-8) of CID=1; At CID=2,3 ..., in the detection site (4-8) of N-1, inject different sample solutions to be detected; Then kit is placed on the disk-shaped support (6) of detection platform, carries out next step;

Step 102, adjustment RGB primaries source strength:

The material detected as required, user interactive module (9) arranges RGB three primary colors light source by message processing module, i.e. the intensity of light source R (6-1), light source G (6-2) and light source B (6-3); The intensity of photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12) difference probe source R (6-1), light source G (6-2) and light source B (6-3), and the strength information of RGB three primary colors light source is passed to message processing module; The intensity signal that message processing module is arranged according to the strength information of the RGB three primary colors light source received and user interactive module, the intensity of feedback regulation RGB three primary colors light source, makes the wavelength band of detection spectral line be applicable to material to be detected;

Step 103, laser positioning initial position:

User interactive module (9) is rotated by message processing module control step motor driver plate shape support (6), the through hole 14 at zero point of the laser positioning hole at zero point (4-4) of the laser light kit (4) that laser instrument I (2) is sent and detection platform (5), is irradiated in photoresistance I (12); Now photoresistance I (12) detects the laser intensity data I obtained and passes to user interactive module (9) by message processing module; Initial position is confirmed after user interactive module (9) receives laser intensity data I; Then next step is performed;

Step 104, initial position detection:

When initial position, detect after detection site (4-8) that light is irradiated to the CID=0 of kit (4) detects, be irradiated in detecting sensor (10) by the detection through hole of detection platform (5); Detecting sensor (10) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains RGB tri-color channel data of the detection site (4-8) of CID=0 according to spectral information, be designated as R [0] respectively, G [0] and B [0];

After the verification site (4-3) that verification light is irradiated to the YID=0 of kit (4) is detected, be irradiated on check sensor (11) by the verification through hole of detection platform (5); Check sensor (11) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the RGB tri-color channel data sum in the verification site (4-3) of YID=0 according to spectral information, be designated as Ref [0];

Step 105, rotation location:

Message processing module control step motor rotates degree, after having rotated, message processing module judges whether the photoresistance II (13) corresponding with positioning through hole 15 detects laser intensity data II;

If do not detect laser intensity data II, then illustrate that larger position skew occurs kit in rotary course, message processing module gives a warning to user interactive module, and user carries out the position correction of kit;

If detect laser intensity data II, then illustrate that the position of kit does not offset, carry out next step;

Step 106, obtain current detection site and verification site data:

Message processing module, according to the method in step 104, obtains current detection site RGB tri-color channel data, is designated as R [CID] respectively, G [CID], B [CID], and three, current verification site color channel data sum, be designated as Ref [YID];

Step 107, repetition step 105 and step 106, until detected all detection site that sample solution is housed; Obtain Ref [the 0] ~ Ref [N-1] in RGB tri-color channel data R [0] ~ R [N-1], the G [0] of each detection site ~ G [N-1] and B [0] ~ B [N-1] and each verification site;

In step 108, different sample, the concentration of allied substances calculates:

First according to two groups of data of two detection site of CID_=0 and CID=1, i.e. R [0], G [0], B [0] and R [1], G [1], B [1] determines the RGB three primary colors typical curve needing detection material;

For other detection site, a color channel data maximum in RGB tri-color channel data of each detection site is updated in RGB three primary colors typical curve, obtains the concentration needing detection material in current detection site in sample; Simultaneously according to this concentration and RGB three primary colors typical curve, obtain the theoretical light intensity values of other two Color Channels; Then the difference between the theoretical light intensity values of other two Color Channels and the light intensity value detected is calculated respectively; If the difference of two Color Channels is all within error threshold, then detect successfully, testing result is sent to user interactive module (9) with the form of " sample No. ID+material concentration " by message processing module; If the difference of two Color Channels has exceed error threshold, then prove also to have other unknown materials in the sample in current detection site, message processing module sends to user interactive module (9) with the form of " No. ID, sample+material concentration+warning message ";

Step 109, calculating Detection accuracy:

For testing result Ref [the 0] ~ Ref [N-1] of verification site (4-3), message processing module calculates the mean value of Ref [0] ~ Ref [N-1]; Then after making maximal value wherein and minimum value subtract each other, divided by this mean value, obtain the metrical error η of described Systems for optical inspection, and send to user interactive module user interactive module (9); User interactive module (9) is according to the accuracy of the testing result in error η analytical procedure 108.

A detection method for described Systems for optical inspection, in this detection method sample of the same race, the detection of material concentration not of the same race, comprises the steps:

Step 201, injection reagent:

First, in the verification site (4-3) of kit (4), the volumetric solution of various material to be detected and the light reaction reagent of respective substance is injected; Meanwhile, inject identical light reaction reagent verifying in the corresponding detection site (4-8) in site (4-3) with each, and carry out immobilization process;

Then, sample solution is injected in the detection site (4-8) of kit by detecting thing entrance (4-7); Kit is placed on disk-shaped support (6), performs next step;

Step 202, adjustment RGB primaries source strength:

The material detected as required, user interactive module (9) arranges RGB three primary colors light source by message processing module, i.e. the intensity of light source R (6-1), light source G (6-2) and light source B (6-3); The intensity of photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12) difference probe source R (6-1), light source G (6-2) and light source B (6-3), and the strength information of RGB three primary colors light source is passed to message processing module; The intensity signal that message processing module is arranged according to the strength information of the RGB three primary colors light source received and user interactive module, the intensity of feedback regulation RGB three primary colors light source, makes the wavelength band of detection spectral line be applicable to material to be detected;

Step 203, laser positioning initial position:

User interactive module (9) is rotated by message processing module control step motor driver plate shape support (6), make laser instrument I (2) through the through hole at zero point (14) of kit (4) laser positioning hole at zero point (4-4) and detection platform (5), be irradiated in photoresistance I (12); Now photoresistance I (12) detects the laser intensity data I obtained and passes to user interactive module (9) by message processing module; Initial position is confirmed after user interactive module (9) receives laser intensity data I; Then next step is performed;

The detection site (4-8) of laser positioning hole at zero point (4-4) same diametrically far-end is labeled as CID=0; All the other detection site (4-8) are labeled as CID=1,2,3 in order successively ..., N-1, N are the sum of detection site (4-8); The each verification site (4-3) corresponding with each detection site (4-8) is labeled as YID=0,1 ..., N-1, then performs next step;

Step 204, initial position detection:

When initial position, detect after detection site (4-8) that light is irradiated to the CID=0 of kit (4) detects, be irradiated in detecting sensor (10) by the detection through hole of detection platform; Detecting sensor (10) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the light intensity data of the detection site (4-8) of CID=0 according to spectral information, be designated as Sam [0];

After the verification site (4-3) that verification light is irradiated to the YID=0 of kit (4) is detected, be irradiated on check sensor (11) by the verification through hole (15) of detection platform (5); Check sensor (11) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the light intensity data in the verification site (4-3) of YID=0 according to spectral information, be designated as: Re [0];

Step 205, rotation location:

Message processing module control step motor rotates degree, after having rotated, message processing module judges whether the photoresistance II (13) below laser positioning hole detects laser intensity data II;

If do not detect laser intensity data II, then illustrate that larger position skew occurs kit in rotary course, message processing module gives a warning to user interactive module, and user carries out the position correction of kit;

If detect laser intensity data II, then illustrate that the position of kit does not offset, carry out next step;

Step 206, obtain current detection site and verification site data:

The CID=x in note current detection site, the YID=x in current verification site, obtains the light intensity data Sam [x] in current detection site (4-8) and the light intensity data Re [x] in current verification site (4-3) according to the method identical with step 204;

Step 207, repetition step 205 and step 206, until detected all detection site that sample solution is housed; Obtain the light intensity data Sam [CID] of each detection site (4-8) and the light intensity data Re [YID] of each verification site (4-3);

Step 208, acquisition testing result:

According to the attribute of light reaction reagent, the size of the light intensity data Sam [CID] of more corresponding detection site (4-8) and the light intensity data Re [YID] in verification site, thus judge whether the concentration of material to be detected exceedes threshold value, and testing result is sent in the mode of " material title+whether exceed threshold value " be given to user interactive module.

The present invention has following beneficial effect:

1, kit processing structure of the present invention, can be fixed on reagent easily in disc kit, not only simplify operation steps required when user detects, can also be applicable to the detection of the different material in multiple situation.

2, adopt magnetic to fix and laser detection locates the means combined, simplify user in testing process kit is fixed in operation; Adopt small rotary formula kit, only need control the anglec of rotation variable during detection, simplify the complexity of system, increase the stability of system; Employ the light source that three Color Channels can be independently adjustable respectively, make system by realizing the detection to different material to the different optimum configurations of three colour light sources and feedback regulation.

3, the present invention is directed to Systems for optical inspection and devise two kinds of different detection methods, make a kind of hardware optics system can towards multiple user; In to different sample during the detection of allied substances concentration, by the calculating to Detection accuracy, enhance the confidence level of testing result; Have employed the integration system of optics, robotization and wireless communication module, make user that other mobile terminals can be used to carry out control and personalized adjustment to each attribute detected and parameter, and the detection of many flux can be carried out at non-laboratory situations to sample.

Accompanying drawing explanation

Fig. 1 is the structure of kit channel layer.

Fig. 2 is the structure of kit top layer.

Fig. 3 is the structure of kit functional layer.

Fig. 4 is the structure of kit bottom.

Fig. 5 is the schematic diagram of the Systems for optical inspection of kit.

Fig. 6 is the Cleaning Principle figure of the Systems for optical inspection of kit.

Fig. 7 is the triple channel half-mark directrix curve of uranin concentration.

Wherein, 1-Optical system module, 2-laser instrument, 3-laser instrument, 4-kit, 5-detection platform, 6-disk-shaped support, 7-stepper motor, 8-bottom circuit board, 9-user interactive module, 10-detecting sensor, 11-check sensor, 12-photoresistance, 13-photoresistance, 14-through hole at zero point, 15-positioning through hole; 4-2-magnet pilot hole, 4-3-verifies site, 4-4-laser positioning hole at zero point, 4-5-laser positioning hole, 4-6-fluid passage, and 4-7-detects thing entrance.

Embodiment

To develop simultaneously embodiment below in conjunction with accompanying drawing, describe the present invention.

As shown in Figure 1, Figure 2, Fig. 3 and Fig. 4 is respectively the structural drawing of kit channel layer of the present invention, top layer, functional layer and bottom;

This kit is four layers of disc-shaped structure, comprises the top layer, channel layer, functional layer and the bottom that adopt the mode of gummed or thermocompression bonding to connect in turn from top to bottom;

Wherein, magnet pilot hole 4-2 is circumferentially evenly processed with around the center of circle of channel layer; Circumferentially evenly circular hole is processed with, as verification site 4-3 in magnet pilot hole 4-2 periphery; The periphery in a verification site is processed with laser positioning hole at zero point 4-4 wherein, and the center of circle of the center of circle of the center of circle of laser positioning hole at zero point 4-4, this verification site 4-3 and channel layer at same diametrically; Circumferentially evenly be processed with and the fluid passage 4-6 verifying site 4-3 equivalent in the periphery of laser positioning hole at zero point 4-4; The agent structure of described fluid passage 4-6 is circular hole, and the edge of circular hole has 2 along same diametric strip channel groove; And the center of circle of the center of circle of each fluid passage 4-6 circular hole and corresponding verification site 4-3 is on the same radius of channel layer; The laser positioning hole 4-5 with fluid passage 4-6 equivalent is evenly processed with in fluid passage 4-6 periphery; And the center of circle of each laser positioning hole 4-5, the center of circle of fluid passage 4-6 circular hole, verification the center of circle of site 4-3 and the center of circle of kit center pit 4-1 kit same diametrically;

Top layer is processed with paired detection thing entrance 4-7, its logarithm is consistent with the number of fluid passage 4-6, and often pair of two circular hole positions detecting thing entrance 4-7 are corresponding with the two ends of the strip channel groove of fluid passage 4-6 respectively; Top layer is also processed with the laser positioning zero point hole 4-4 corresponding with channel layer;

Functional layer is processed with the circular hole corresponding with the agent structure of the fluid passage 4-6 on channel layer, as detection site 4-8; Functional layer is also processed with the magnet pilot hole 4-2 corresponding with channel layer, verification site 4-3, laser positioning hole at zero point 4-4 and laser positioning hole 4-5;

Bottom is processed with the magnet pilot hole 4-2 corresponding with channel layer and laser positioning hole 4-5.

Be illustrated in figure 5 the structural drawing of the Systems for optical inspection based on mentioned reagent box, comprise Optical system module 1, laser instrument I 2, laser instrument II 3, kit 4, detection platform 5, disk-shaped support 6, stepper motor 7, bottom circuit board 8 and user interactive module 9.

Bottom circuit board 8 comprises detecting sensor 10, check sensor 11, photoresistance I 12 and photoresistance II 13 and message processing module; Wherein, between message processing module and user interactive module 9, carry out data interaction, and adopt the communication based on agreement to carry out data interaction.

The circle centre position of detection platform 5 is processed with center hole; Detection platform 5 be also processed with the detection through hole corresponding with detecting sensor 10 and check sensor 11 and verify through hole; Corresponding with photoresistance I 12 and photoresistance II 13 zero point through hole 14 and positioning through hole 15.

The magnet pilot hole 4-2 of kit 4 built with magnet, the radius of a circle that the verification site 4-3 that the radius of disk-shaped support 6 is less than kit 4 is formed;

Detection platform 5 is fixedly mounted on above stepper motor; Disk-shaped support 6 is placed in detection platform 5; Disk-shaped support 6 is connected with disk-shaped support 6 by the center hole of detection platform 5 with the output shaft of stepper motor 7; Kit 4 by magnet adsorption on disk-shaped support 6; The output shaft of stepper motor 7 drives disk-shaped support 6 to rotate, thus drives kit 4 to rotate.

Laser instrument I 2 by laser positioning hole at zero point 4-4 and zero point through hole 14 be irradiated in photoresistance I 12, photoresistance I 12 receives laser, and laser intensity data I are sent to message processing module;

Laser instrument II 3 is irradiated in photoresistance II 13 by the laser positioning hole 4-5 of kit and positioning through hole 15, and photoresistance II 13 receives laser, and intensity data II is sent to message processing module;

Analysis result is sent to user interactive module 9 after analyzing laser intensity data I and intensity data II by message processing module; According to analysis result, user interactive module 9 judges whether kit position skew occurs, the position of timely calibration reagent box when there is position skew.

As shown in Figure 6, Optical system module 1 comprises light source R6-1, light source G6-2, light source B6-3, light splitting piece I 6-4, light splitting piece II 6-5, light splitting piece III 6-6, light splitting piece IV 6-7, light splitting piece V 6-8, collimation lens 6-9, photodiode I 6-10, photodiode II 6-11 and photodiode III 6-12;

Wherein, light splitting piece I 6-4, light splitting piece II 6-5, light splitting piece III 6-6, collimation lens 6-9, light splitting piece IV 6-7 and light splitting piece V 6-8 coaxially place successively; Photodiode I 6-10, photodiode II 6-11 and photodiode III 6-12 are arranged on bottom circuit board 8;

The light that light source R6-1 sends, produce reflection and transmission two light after being irradiated to light splitting piece I 6-4, transmitted ray is irradiated to photodiode I 6-10, the intensity of photodiode I probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source R6-1; Reflection ray is irradiated on collimation lens 6-9 through light splitting piece II 6-5 and light splitting piece III 6-6 successively;

The light that light source G6-2 sends, be irradiated to light splitting piece II 6-5 and produce reflection and transmission two light, transmitted ray is irradiated to photodiode II 6-11, the intensity of photodiode II probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source G6-2; Reflection ray is irradiated on collimation lens 6-9 through light splitting piece III 6-6;

The light that light source B6-3 sends, be irradiated to light splitting piece III 6-6 and produce reflection and transmission two light, transmitted ray is irradiated to photodiode III 6-12, the intensity of photodiode III probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source G6-3; Reflection ray is irradiated on collimation lens 6-9;

Three reflection rays be irradiated on collimation lens 6-9 are pooled a light by described collimation lens 6-9, this light reflects to produce through light splitting piece III 6-7 and detects light, reflects again produce verification light after light splitting piece III 6-7 transmission through light splitting piece IV 6-8.

Detecting light is irradiated in detecting sensor 10 by the detection site 4-8 of one of them kit 4 and the detection through hole of detection platform, and detecting sensor 10 receives and detects light and after obtaining light intensity data I, be transferred to message processing module; Verification light is irradiated on check sensor 11 by the verification site 4-3 of kit 4 and the verification through hole of detection platform, and check sensor 11 receives verification light and after obtaining light intensity data II, is transferred to message processing module;

Message processing module obtains testing result after comprehensively analyzing the light intensity data I of each detection site 4-8 and each light intensity data II verifying site 4-3, and testing result is sent to user interactive module 9.

One, based on the detection detection method to allied substances concentration in different sample of above-mentioned Systems for optical inspection, comprise the steps:

Step 101, injection reagent:

Any solution is not injected in the verification site 4-3 of kit 4; Detection site 4-8 with laser positioning hole at zero point 4-4 same diametrically far-end is labeled as CID=0; Adjacent with the detection site 4-8 of CID=0 detection site 4-8 is labeled as CID=1, all the other detection site 4-8 is labeled as CID=2 in order successively, 3 ..., N-1, N are the sum of detection site 4-8; The each verification site 4-3 corresponding with each detection site 4-8 is labeled as YID=0,1 ..., N-1;

Placebo solution is injected in the detection site 4-8 of CID=0; The contrast solution of concentration known is injected in the detection site 4-8 of CID=1; At CID=2,3 ..., in the detection site 4-8 of N-1, inject different sample solutions to be detected; Then kit is placed on the disk-shaped support 6 of detection platform, carries out next step;

Step 102, adjustment RGB primaries source strength:

The material detected as required, user interactive module 9 arranges RGB three primary colors light source by message processing module, i.e. the intensity of light source R6-1, light source G6-2 and light source B6-3; Photodiode I 6-10, photodiode II 6-11 and photodiode III 6-12 distinguish the intensity of probe source R6-1, light source G6-2 and light source B6-3, and the strength information of RGB three primary colors light source is passed to message processing module; The intensity signal that message processing module is arranged according to the strength information of the RGB three primary colors light source received and user interactive module, the intensity of feedback regulation RGB three primary colors light source, makes the wavelength band of detection spectral line be applicable to material to be detected;

Step 103, laser positioning initial position:

User interactive module 9 is rotated by message processing module control step motor driver plate shape support 6, and the through hole 14 at zero point of laser positioning hole at the zero point 4-4 of the laser light kit 4 that laser instrument I 2 is sent and detection platform 5, is irradiated in photoresistance I 12; Now photoresistance I 12 detects the laser intensity data I obtained and passes to user interactive module 9 by message processing module; User interactive module 9 confirms initial position after receiving laser intensity data I; Then next step is performed;

Step 104, initial position detection:

When initial position, detect after detection site 4-8 that light is irradiated to the CID=0 of kit 4 detects, be irradiated in detecting sensor 10 by the detection through hole of detection platform 5; Detecting sensor 10 detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains RGB tri-color channel data of the detection site 4-8 of CID=0 according to spectral information, be designated as R [0] respectively, G [0] and B [0];

After the verification site 4-3 that verification light is irradiated to the YID=0 of kit 4 detects, be irradiated on check sensor 11 by the verification through hole of detection platform 5; Check sensor 11 detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the RGB tri-color channel data sum of the verification site 4-3 of YID=0 according to spectral information, be designated as Ref [0];

Step 105, rotation location:

Message processing module control step motor rotates degree, after having rotated, message processing module judges whether the photoresistance II 13 corresponding with positioning through hole 15 detects laser intensity data II;

If do not detect laser intensity data II, then illustrate that larger position skew occurs kit in rotary course, message processing module gives a warning to user interactive module, and user carries out the position correction of kit;

If detect laser intensity data II, then illustrate that the position of kit does not offset, carry out next step;

Step 106, obtain current detection site and verification site data:

Message processing module, according to the method in step 104, obtains current detection site RGB tri-color channel data, is designated as R [CID] respectively, G [CID], B [CID], and three, current verification site color channel data sum, be designated as Ref [YID];

Step 107, repetition step 105 and step 106, until detected all detection site that sample solution is housed; Obtain Ref [the 0] ~ Ref [N-1] in RGB tri-color channel data R [0] ~ R [N-1], the G [0] of each detection site ~ G [N-1] and B [0] ~ B [N-1] and each verification site;

In step 108, different sample, the concentration of allied substances calculates:

First according to two groups of data of two detection site of CID_=0 and CID=1, i.e. R [0], G [0], B [0] and R [1], G [1], B [1] determines the RGB three primary colors typical curve needing detection material;

For other detection site, a color channel data maximum in RGB tri-color channel data of each detection site is updated in RGB three primary colors typical curve, obtains the concentration needing detection material in current detection site in sample; Simultaneously according to this concentration and RGB three primary colors typical curve, obtain the theoretical light intensity values of other two Color Channels; Then the difference between the theoretical light intensity values of other two Color Channels and the light intensity value detected is calculated respectively; If the difference of two Color Channels is all within error threshold, then detect successfully, testing result is sent to user interactive module 9 with the form of " sample No. ID+material concentration " by message processing module; If the difference of two Color Channels has exceed error threshold, then prove also to have other unknown materials in the sample in current detection site, message processing module sends to user interactive module 9 with the form of " No. ID, sample+material concentration+warning message ";

Step 109, calculating Detection accuracy:

$\mathrm{\η}=\frac{Max\{\mathrm{Re}f\[0~11\]\}-Min\{\mathrm{Re}f\[0~11\]\}}{\underset{n=0}{\overset{11}{\Σ}}\mathrm{Re}f\[n\]/12}\×100\%---\left(1\right)$

In formula (1), Ref [0 ~ 11] is ID_Y=0,1 ... three color channel data sums of the verification site 4-3 of 11.

Namely for testing result Ref [the 0] ~ Ref [N-1] of verification site 4-3, message processing module calculates the mean value of Ref [0] ~ Ref [N-1]; Then after making maximal value wherein and minimum value subtract each other, divided by this mean value, obtain the metrical error η of described Systems for optical inspection, and send to user interactive module user interactive module 9; User interactive module 9 is according to the accuracy of the testing result in error η analytical procedure 108.

Two, based on the detection method of Systems for optical inspection to material concentration not of the same race in sample of the same race, comprise the steps:

Step 201, injection reagent:

First, in the verification site 4-3 of kit 4, the volumetric solution of various material to be detected and the light reaction reagent of respective substance is injected; Meanwhile, inject identical light reaction reagent verifying in the corresponding detection site 4-8 of site 4-3 with each, and carry out immobilization process;

Then, sample solution is injected in the detection site 4-8 of kit by detecting thing entrance 4-7; Kit is placed on disk-shaped support 6, performs next step;

Step 202, adjustment RGB primaries source strength:

The material detected as required, user interactive module 9 arranges RGB three primary colors light source by message processing module, i.e. the intensity of light source R6-1, light source G6-2 and light source B6-3; Photodiode I 6-10, photodiode II 6-11 and photodiode III 6-12 distinguish the intensity of probe source R6-1, light source G6-2 and light source B6-3, and the strength information of RGB three primary colors light source is passed to message processing module; The intensity signal that message processing module is arranged according to the strength information of the RGB three primary colors light source received and user interactive module, the intensity of feedback regulation RGB three primary colors light source, makes the wavelength band of detection spectral line be applicable to material to be detected;

Step 203, laser positioning initial position:

User interactive module 9 is rotated by message processing module control step motor driver plate shape support 6, makes laser instrument I 2 through the through hole 14 at zero point of kit 4 laser positioning hole at zero point 4-4 and detection platform 5, is irradiated in photoresistance I 12; Now photoresistance I 12 detects the laser intensity data I obtained and passes to user interactive module 9 by message processing module; User interactive module 9 confirms initial position after receiving laser intensity data I; Then next step is performed;

The detection site 4-8 of laser positioning hole at zero point 4-4 same diametrically far-end is labeled as CID=0; All the other detection site 4-8 are labeled as CID=1 in order successively, 2,3 ..., N-1, N are the sum of detection site 4-8; The each verification site 4-3 corresponding with each detection site 4-8 is labeled as YID=0,1 ..., N-1, then performs next step;

Step 204, initial position detection:

When initial position, detect after detection site 4-8 that light is irradiated to the CID=0 of kit 4 detects, be irradiated in detecting sensor 10 by the detection through hole of detection platform; Detecting sensor 10 detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the light intensity data of the detection site (4-8) of CID=0 according to spectral information, be designated as Sam [0];

After the verification site 4-3 that verification light is irradiated to the YID=0 of kit 4 detects, be irradiated on check sensor 11 by the verification through hole 15 of detection platform 5; Check sensor 11 detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the light intensity data of the verification site 4-3 of YID=0 according to spectral information, be designated as: Re [0];

Step 205, rotation location:

Message processing module control step motor rotates degree, after having rotated, message processing module judges whether the photoresistance II 13 below laser positioning hole detects laser intensity data II;

If do not detect laser intensity data II, then illustrate that larger position skew occurs kit in rotary course, message processing module gives a warning to user interactive module, and user carries out the position correction of kit;

If detect laser intensity data II, then illustrate that the position of kit does not offset, carry out next step;

Step 206, obtain current detection site and verification site data:

The CID=x in note current detection site, the YID=x in current verification site, obtains the light intensity data Sam [x] of current detection site 4-8 and the light intensity data Re [x] of current verification site 4-3 according to the method identical with step 204;

Step 207, repetition step 205 and step 206, until detected all detection site that sample solution is housed; Obtain the light intensity data Sam [CID] of each detection site 4-8 and the light intensity data Re [YID] of each verification site 4-3;

Step 208, acquisition testing result:

According to the attribute of light reaction reagent, the size of the light intensity data Sam [CID] of more corresponding detection site 4-8 and the light intensity data Re [YID] in verification site, thus judge whether the concentration of material to be detected exceedes threshold value, and testing result is sent in the mode of " material title+whether exceed threshold value " be given to user interactive module.

A kind of actual application scheme of the present invention can be: such as the detection of heavy metal ion in current family expenses and environment water, the kit of native system can adopt in A detection scheme equivalent water body sample with for a certain heavy metal ion aptamers mixed solution as detecting sample, in B detection scheme, aptamers for different heavy metal example also can be adopted as detecting reagent.The light source of native system can adopt RGB primitive colours LED.The processor meeting systemic-function can select the microprocessor of 8051, MSP430 and ARM kernel.The CCD of system can select the single pixel CCD of the TCS32X0 of TAOS company series.The wireless chip of data input and output can adopt serial ports-bluetooth, the protocol conversion chips such as SPI-WIFI.

In sum, these are only preferred embodiment of the present invention, be not intended to limit protection scope of the present invention.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (10)

1., for the kit that material detects, it is characterized in that:

This kit is four layers of disc-shaped structure, comprises the top layer, channel layer, functional layer and the bottom that connect in turn from top to bottom;

Wherein, around the center of circle of described channel layer, be circumferentially evenly processed with circular hole, as verification site (4-3); The periphery of verification site (4-3) is circumferentially evenly processed with and the fluid passage (4-6) verifying site (4-3) equivalent; The agent structure of described fluid passage (4-6) is circular hole, and the edge of circular hole has 2 along same diametric strip channel groove; And the center of circle in the center of circle of each fluid passage (4-6) circular hole and corresponding verification site (4-3) is on the same radius of channel layer;

Described top layer is processed with paired detection thing entrance (4-7), its logarithm is consistent with the number of fluid passage (4-6), and often pair of two circular hole positions detecting thing entrance (4-7) are corresponding with the two ends of the strip channel groove of fluid passage (4-6) respectively;

Described functional layer is processed with the circular hole corresponding with the agent structure of the fluid passage (4-6) on channel layer, as detection site (4-8); Functional layer is also processed with the verification site (4-3) corresponding with channel layer.

2. a kind of kit detected for material as claimed in claim 1, is characterized in that, adopt gummed or the mode of thermocompression bonding to connect between four layers of described kit.

3. the Systems for optical inspection based on the kit described in claim 1 or 2, it is characterized in that, comprise Optical system module (1), kit (4), stepper motor (7), bottom circuit board (8) and user interactive module (9);

Described bottom circuit board (8) comprises detecting sensor (10), check sensor (11) and message processing module;

Described stepper motor (7) drives the center of circle of kit (4) to rotate;

Described Optical system module (1) produces two identical light, and a conduct detects light, and another is as verification light; Detecting light is irradiated in detecting sensor (10) by one of them detection site (4-8), and detecting sensor (10) receives and detects light and after obtaining light intensity data I, be transferred to message processing module; Verification light is irradiated on check sensor (11) by the verification site (4-3) of kit, and check sensor (11) receives verification light and after obtaining light intensity data II, is transferred to message processing module;

Message processing module obtains testing result after comprehensively analyzing the light intensity data I of each detection site (4-8) and the light intensity data II of each verification site (4-3), and testing result is sent to user interactive module (9).

4. a kind of Systems for optical inspection based on kit as claimed in claim 3, it is characterized in that, described Systems for optical inspection also comprises detection platform (5) and disk-shaped support (6);

The radius of a circle that the verification site (4-3) that the radius of described disk-shaped support (6) is less than described kit (4) is formed; The circle centre position of described detection platform (5) is processed with center hole; Detection platform (5) be also processed with the detection through hole corresponding with detecting sensor (10) and check sensor (11) and verify through hole;

Described detection platform (5) is fixedly mounted on above stepper motor; Described disk-shaped support (6) is placed in detection platform (5); Disk-shaped support (6) is connected with disk-shaped support (6) by the center hole of detection platform (5) with the output shaft of stepper motor (7); Described kit (4) is fixed on disk-shaped support (6); The output shaft of stepper motor (7) drives disk-shaped support (6) to rotate, thus drives kit (4) to rotate.

5. a kind of Systems for optical inspection based on kit as claimed in claim 4, is characterized in that, the channel layer of described kit (4) is also processed with magnet pilot hole (4-2) around the center of circle; And functional layer is also processed with the magnet pilot hole (4-2) corresponding with channel layer; Magnet pilot hole (4-2) built with magnet, kit (4) by magnet adsorption on disk-shaped support (6).

6. a kind of Systems for optical inspection based on kit as claimed in claim 5, is characterized in that:

On the channel layer of described kit (4), the periphery in one of them verification site is processed with laser positioning hole at zero point (4-4), and the center of circle of the center of circle in the center of circle in this laser positioning hole at zero point (4-4), corresponding verification site (4-3) and channel layer at same diametrically; The laser positioning hole (4-5) with fluid passage (4-6) equivalent is evenly processed with in fluid passage (4-6) periphery; And the center of circle of each laser positioning hole (4-5), the center of circle of fluid passage (4-6) circular hole, verification site (4-3) the center of circle and kit center pit (4-1) the center of circle kit same diametrically; In addition, on described bottom, the laser positioning zero point hole (4-4) corresponding with channel layer and laser positioning hole (4-5) is processed with;

Described Systems for optical inspection (1) also comprises laser instrument I (2), laser instrument II (3), photoresistance I (12) and photoresistance II (13);

Photoresistance I (12) and photoresistance II (13) are arranged on described bottom circuit board (8); Detection platform (5) is upper also has the zero point through hole (14) corresponding with photoresistance I (12) and photoresistance II (13) and positioning through hole (15);

Laser instrument I (2) is by the laser positioning hole at zero point (4-4) of kit and be irradiated to through hole at zero point (14) in photoresistance I (12), photoresistance I (12) receives laser, and laser intensity data I are sent to message processing module;

Laser instrument II (3) is irradiated in photoresistance II (13) by the laser positioning hole (4-5) of kit and positioning through hole (15), photoresistance II (13) receives laser, and intensity data II is sent to message processing module;

Analysis result is sent to user interactive module (9) after analyzing laser intensity data I and intensity data II by message processing module; According to analysis result, user interactive module (9) judges whether kit position skew occurs, the position of timely calibration reagent box when there is position skew.

7. a kind of Systems for optical inspection based on kit as claimed in claim 6, it is characterized in that, described Optical system module (1) comprises light source R (6-1), light source G (6-2), light source B (6-3), light splitting piece I (6-4), light splitting piece II (6-5), light splitting piece III (6-6), light splitting piece IV (6-7), light splitting piece V (6-8), collimation lens (6-9), photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12);

Wherein, light splitting piece I (6-4), light splitting piece II (6-5), light splitting piece III (6-6), collimation lens (6-9), light splitting piece IV (6-7) and light splitting piece V (6-8) are coaxially placed successively; Photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12) are arranged on bottom circuit board (8);

The light that light source R (6-1) sends, be irradiated to light splitting piece I (6-4) and produce reflection and transmission two light afterwards, transmitted ray is irradiated to photodiode I (6-10), the intensity of photodiode I probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source R (6-1); Reflection ray is irradiated on collimation lens (6-9) through light splitting piece II (6-5) and light splitting piece III (6-6) successively;

The light that light source G (6-2) sends, be irradiated to light splitting piece II (6-5) and produce reflection and transmission two light, transmitted ray is irradiated to photodiode II (6-11), the intensity of photodiode II probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source G (6-2); Reflection ray is irradiated on collimation lens (6-9) through light splitting piece III (6-6);

The light that light source B (6-3) sends, be irradiated to light splitting piece III (6-6) and produce reflection and transmission two light, transmitted ray is irradiated to photodiode III (6-12), the intensity of photodiode III probing light, and light intensity is sent to data processing module; Data processing module is according to the light intensity of light intensity feedback regulation light source G (6-3); Reflection ray is irradiated on collimation lens (6-9);

Three reflection rays that described collimation lens (6-9) will be irradiated on collimation lens (6-9) pool a light, this light produces through light splitting piece III (6-7) reflection and detects light, after light splitting piece III (6-7) transmission, produce verification light again through light splitting piece IV (6-8) reflection.

8. a kind of Systems for optical inspection based on kit as described in claim 3 or 4 or 5 or 6 or 7, it is characterized in that, carry out data between message processing module and user interactive module (9) when transmitting, adopt the communication based on agreement to carry out.

9., based on a detection method for Systems for optical inspection according to claim 7, this detection method, for the detection of allied substances concentration in different sample, is characterized in that, comprises the steps:

Step 101, injection reagent:

Any solution is not injected in the verification site (4-3) of kit (4); Detection site (4-8) with laser positioning hole at zero point (4-4) same diametrically far-end is labeled as CID=0; A detection site (4-8) adjacent with the detection site (4-8) of CID=0 is labeled as CID=1, all the other detection site (4-8) are labeled as CID=2 in order successively, 3 ..., N-1, N are the sum of detection site (4-8); The each verification site (4-3) corresponding with each detection site (4-8) is labeled as YID=0,1 ..., N-1;

Placebo solution is injected in the detection site (4-8) of CID=0; The contrast solution of concentration known is injected in the detection site (4-8) of CID=1; At CID=2,3 ..., in the detection site (4-8) of N-1, inject different sample solutions to be detected; Then kit is placed on the disk-shaped support (6) of detection platform, carries out next step;

Step 102, adjustment RGB primaries source strength:

The material detected as required, user interactive module (9) arranges RGB three primary colors light source by message processing module, i.e. the intensity of light source R (6-1), light source G (6-2) and light source B (6-3); The intensity of photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12) difference probe source R (6-1), light source G (6-2) and light source B (6-3), and the strength information of RGB three primary colors light source is passed to message processing module; The intensity signal that message processing module is arranged according to the strength information of the RGB three primary colors light source received and user interactive module, the intensity of feedback regulation RGB three primary colors light source, makes the wavelength band of detection spectral line be applicable to material to be detected;

Step 103, laser positioning initial position:

User interactive module (9) is rotated by message processing module control step motor driver plate shape support (6), the through hole 14 at zero point of the laser positioning hole at zero point (4-4) of the laser light kit (4) that laser instrument I (2) is sent and detection platform (5), is irradiated in photoresistance I (12); Now photoresistance I (12) detects the laser intensity data I obtained and passes to user interactive module (9) by message processing module; Initial position is confirmed after user interactive module (9) receives laser intensity data I; Then next step is performed;

Step 104, initial position detection:

When initial position, detect after detection site (4-8) that light is irradiated to the CID=0 of kit (4) detects, be irradiated in detecting sensor (10) by the detection through hole of detection platform (5); Detecting sensor (10) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains RGB tri-color channel data of the detection site (4-8) of CID=0 according to spectral information, be designated as R [0] respectively, G [0] and B [0];

After the verification site (4-3) that verification light is irradiated to the YID=0 of kit (4) is detected, be irradiated on check sensor (11) by the verification through hole of detection platform (5); Check sensor (11) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the RGB tri-color channel data sum in the verification site (4-3) of YID=0 according to spectral information, be designated as Ref [0];

Step 105, rotation location:

Message processing module control step motor rotates degree, after having rotated, message processing module judges whether the photoresistance II (13) corresponding with positioning through hole 15 detects laser intensity data II;

If do not detect laser intensity data II, then illustrate that larger position skew occurs kit in rotary course, message processing module gives a warning to user interactive module, and user carries out the position correction of kit;

If detect laser intensity data II, then illustrate that the position of kit does not offset, carry out next step;

Step 106, obtain current detection site and verification site data:

Message processing module, according to the method in step 104, obtains current detection site RGB tri-color channel data, is designated as R [CID] respectively, G [CID], B [CID], and three, current verification site color channel data sum, be designated as Ref [YID];

Step 107, repetition step 105 and step 106, until detected all detection site that sample solution is housed; Obtain Ref [the 0] ~ Ref [N-1] in RGB tri-color channel data R [0] ~ R [N-1], the G [0] of each detection site ~ G [N-1] and B [0] ~ B [N-1] and each verification site;

In step 108, different sample, the concentration of allied substances calculates:

First according to two groups of data of two detection site of CID_=0 and CID=1, i.e. R [0], G [0], B [0] and R [1], G [1], B [1] determines the RGB three primary colors typical curve needing detection material;

For other detection site, a color channel data maximum in RGB tri-color channel data of each detection site is updated in RGB three primary colors typical curve, obtains the concentration needing detection material in current detection site in sample; Simultaneously according to this concentration and RGB three primary colors typical curve, obtain the theoretical light intensity values of other two Color Channels; Then the difference between the theoretical light intensity values of other two Color Channels and the light intensity value detected is calculated respectively; If the difference of two Color Channels is all within error threshold, then detect successfully, testing result is sent to user interactive module (9) with the form of " sample No. ID+material concentration " by message processing module; If the difference of two Color Channels has exceed error threshold, then prove also to have other unknown materials in the sample in current detection site, message processing module sends to user interactive module (9) with the form of " No. ID, sample+material concentration+warning message ";

Step 109, calculating Detection accuracy:

For testing result Ref [the 0] ~ Ref [N-1] of verification site (4-3), message processing module calculates the mean value of Ref [0] ~ Ref [N-1]; Then after making maximal value wherein and minimum value subtract each other, divided by this mean value, obtain the metrical error η of described Systems for optical inspection, and send to user interactive module user interactive module (9); User interactive module (9) is according to the accuracy of the testing result in error η analytical procedure 108.

10., based on a detection method for Systems for optical inspection according to claim 7, in this detection method sample of the same race, the detection of material concentration not of the same race, is characterized in that, comprise the steps:

Step 201, injection reagent:

First, in the verification site (4-3) of kit (4), the volumetric solution of various material to be detected and the light reaction reagent of respective substance is injected; Meanwhile, inject identical light reaction reagent verifying in the corresponding detection site (4-8) in site (4-3) with each, and carry out immobilization process;

Then, sample solution is injected in the detection site (4-8) of kit by detecting thing entrance (4-7); Kit is placed on disk-shaped support (6), performs next step;

Step 202, adjustment RGB primaries source strength:

The material detected as required, user interactive module (9) arranges RGB three primary colors light source by message processing module, i.e. the intensity of light source R (6-1), light source G (6-2) and light source B (6-3); The intensity of photodiode I (6-10), photodiode II (6-11) and photodiode III (6-12) difference probe source R (6-1), light source G (6-2) and light source B (6-3), and the strength information of RGB three primary colors light source is passed to message processing module; The intensity signal that message processing module is arranged according to the strength information of the RGB three primary colors light source received and user interactive module, the intensity of feedback regulation RGB three primary colors light source, makes the wavelength band of detection spectral line be applicable to material to be detected;

Step 203, laser positioning initial position:

User interactive module (9) is rotated by message processing module control step motor driver plate shape support (6), make laser instrument I (2) through the through hole at zero point (14) of kit (4) laser positioning hole at zero point (4-4) and detection platform (5), be irradiated in photoresistance I (12); Now photoresistance I (12) detects the laser intensity data I obtained and passes to user interactive module (9) by message processing module; Initial position is confirmed after user interactive module (9) receives laser intensity data I; Then next step is performed;

The detection site (4-8) of laser positioning hole at zero point (4-4) same diametrically far-end is labeled as CID=0; All the other detection site (4-8) are labeled as CID=1,2,3 in order successively ..., N-1, N are the sum of detection site (4-8); The each verification site (4-3) corresponding with each detection site (4-8) is labeled as YID=0,1 ..., N-1, then performs next step;

Step 204, initial position detection:

When initial position, detect after detection site (4-8) that light is irradiated to the CID=0 of kit (4) detects, be irradiated in detecting sensor (10) by the detection through hole of detection platform; Detecting sensor (10) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the light intensity data of the detection site (4-8) of CID=0 according to spectral information, be designated as Sam [0];

After the verification site (4-3) that verification light is irradiated to the YID=0 of kit (4) is detected, be irradiated on check sensor (11) by the verification through hole (15) of detection platform (5); Check sensor (11) detection obtains spectral information, and spectral information is passed to message processing module; Message processing module obtains the light intensity data in the verification site (4-3) of YID=0 according to spectral information, be designated as: Re [0];

Step 205, rotation location:

Message processing module control step motor rotates degree, after having rotated, message processing module judges whether the photoresistance II (13) below laser positioning hole detects laser intensity data II;

If do not detect laser intensity data II, then illustrate that larger position skew occurs kit in rotary course, message processing module gives a warning to user interactive module, and user carries out the position correction of kit;

If detect laser intensity data II, then illustrate that the position of kit does not offset, carry out next step;

Step 206, obtain current detection site and verification site data:

The CID=x in note current detection site, the YID=x in current verification site, obtains the light intensity data Sam [x] in current detection site (4-8) and the light intensity data Re [x] in current verification site (4-3) according to the method identical with step 204;

Step 207, repetition step 205 and step 206, until detected all detection site that sample solution is housed; Obtain the light intensity data Sam [CID] of each detection site (4-8) and the light intensity data Re [YID] of each verification site (4-3);

Step 208, acquisition testing result:

According to the attribute of light reaction reagent, the size of the light intensity data Sam [CID] of more corresponding detection site (4-8) and the light intensity data Re [YID] in verification site, thus judge whether the concentration of material to be detected exceedes threshold value, and testing result is sent in the mode of " material title+whether exceed threshold value " be given to user interactive module.