CN105040119A - Preparation method for polyacrylonitrile nanometer electrospinning fibrous membrane for slow-release anti-herpes virus drugs - Google Patents
Preparation method for polyacrylonitrile nanometer electrospinning fibrous membrane for slow-release anti-herpes virus drugs Download PDFInfo
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- CN105040119A CN105040119A CN201510310138.6A CN201510310138A CN105040119A CN 105040119 A CN105040119 A CN 105040119A CN 201510310138 A CN201510310138 A CN 201510310138A CN 105040119 A CN105040119 A CN 105040119A
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Abstract
The invention discloses a preparation method for a polyacrylonitrile nanometer electrospinning fibrous membrane for slow-release anti-herpes virus drugs. The preparation method comprises the following steps of dissolving acyclovir in an organic solvent to obtain an acyclovir solution; then, adding dry polyacrylonitrile powder to the acyclovir solution, and dissolving to obtain electrospinning solution; pouring the prepared solution into a solution holder, and putting the holder on a micro-injection pump; connecting a truncated syringe needle as a capillary tube for jetting capillary flow to the anode of a high voltage power supply, and connecting an aluminum foil fiber receiving flat to the cathode, and turning on the power supply; performing electrospinning according to a suitable electrospinning condition to obtain the polyacrylonitrile nanometer electrospinning fibrous membrane for the slow-release anti-herpes virus drugs. The method disclosed by the method is simple, safe, novel and efficient, and has a very good application prospect in the aspect of anti-herpes virus treatment; the polyacrylonitrile nanometer electrospinning fibrous membrane for the slow-release anti-herpes virus drugs is capable of realizing the slow release of anti-herpes drugs, and can be applied to drug release characteristic study of medical drug-loaded fibers and the specific treatment of herpes viruses.
Description
Technical field
The invention belongs to the textile material preparation field of drug containing, particularly a kind of preparation method of polyacrylonitrile nano electrospun fiber membrane of slowly-releasing anti-herpesvirus medicinal.
Background technology
Bleb, broadly refers to herpes virus coe virus associated diseases.Known at present have eight kinds of viruses to cause human diseases in this section, and this viroid is collectively referred to as nerpes vinrus hominis.Wherein common are varicella virus, herpes simplex virus etc.The multiple organ of human body can be invaded.
ACV is a kind of purine nucleoside analogs of synthesis.Be mainly used in the various infection caused by herpes simplex virus, can be used for onset or recurring skin, mucous membrane, the HSV that external genital organs infects and immune deficiency person occurs infects.For the choice drug for the treatment of HSV encephalitis, the minimizing incidence of disease and the reduction death rate are all better than arabinosy ladenosine.Also can be used for herpes zoster, Epstein-Barr virus, and the infection such as the concurrent varicella of immune deficiency person.Local is only for skin.In the cell of HSV, VZV and ebv infection, by virus-specific t K enzyme, ACV phosphoric acid is turned to mono-phosphorylated compound, then turned to triphosphoric acid compound by cell kinase phosphoric acid, be combined with HSV-DNA polymerase, mix HSV-DNA, stop HSV-DNA chain extension, blocking virus copies.
Any through chemistry, biosynthesis or extraction, exquisite medicine, all making for before Clinical practice the form being suitable for medical diagnosis and prophylactic applications, be called formulation.Formulation known at present has the types such as tablet, injection, suppository, ointment.Along with the development of science and technology, interdisciplinary continuous infiltration is that pharmaceutical preparation provides many referential new methods and new technology.Early 1970s, the research of pharmaceutical dosage form and preparation starts progressively to go deep into the delivery system epoch.Along with the development of pharmacy and fiber spinning technology, there is the pharmaceutical dosage form drug-loading fibre one by one using fiber as carrier.According to the difference of fiber producing processes, drug-loading fibre is roughly divided into solution and spins fiber by us, molten spinning fiber, doughnut, ion-exchange fibre, electrostatic spinning fiber five type.
Wherein, the advantage of electrostatic spinning is that nanofiber has very large specific area, medicine is wherein with the even molecular dispersed state existence of minimum crystal grain state, unformed state, therefore be easy to the various instant DDS fast of preparation further, be also very suitable for the solid dispersions preparing insoluble medicine.Its technical advantage is embodied in the aspects such as technical process is simple, manipulation is convenient, manipulate fibre diameter by change solution concentration and surface tension, selection material is in extensive range; Electrostatic spinning easily can prepare nanosized polymer fiber simultaneously, is be hopeful one of method realizing continuous superfine fibre suitability for industrialized production most
[1-6].
[1] Wu Xiaohui, Wang Linge, Huang Yong. Electrospinning prepares the research of medicament slow release Ethyl Cellulose Fibers. macromolecule journal, 2006, (2): 264-268.
[2]HuangZM,HeCL,YangAZ,eta1.Encapsulatingdrugsinbiodegradableultrafinefibersthroughco-axialelectrospinning.JBiomedMaterRes,2006,77A(1):169.
[3]ZengJ,XuXY,ChenXS,etal.Biodegradableelectrospunfibersfordrugdelivery.JControlRelease,2003,92(3):227-231.
[4]XuX,YangL,XuXY,etal.UltrafinemedicatedfiberselectrospunfromW/Oemulsions.JControlRelease,2005,108(1-2):33.
[5]TaepaiboonP,RungsardthongU,SupapholP.Effectofcross-linkingonpropertiesandreleasecharacteristicsofsodiumsalicylate-loadedelectrospunpoly(vinylalcohol)fibremats.Nanotechnology2007,InPress.
[6]KenawyER,Abdel-HayFI,El-NewehyMH,etal.Controlledreleaseofketoprofenfromelectrospunpoly(vinylalcohol)nanofibers.MaterSciEngA,2007,459(1-2):390.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of polyacrylonitrile nano electrospinning fibre membrane preparation method of slowly-releasing anti-herpesvirus medicinal.The inventive method is simple, safety, novel, efficiently, in anti-herpesvirus treatment, has good application prospect; The polyacrylonitrile nano electrospun fiber membrane of the slowly-releasing anti-herpesvirus medicinal of gained of the present invention can realize the slowly-releasing of antiherpetic thing, can be applied to the drug release characteristics research of medical drug-loading fibre and the immunotherapy targeted autoantibody of herpes-like virus.
The polyacrylonitrile nano electrospinning fibre membrane preparation method of a kind of slowly-releasing anti-herpesvirus medicinal of the present invention, is characterized in that: described anti-herpesvirus medicinal is ACV.
beneficial effect of the present invention is as follows:
(1) the inventive method is simple, safety, novel, efficiently, in anti-herpesvirus treatment, has good application prospect;
(2) the polyacrylonitrile nano electrospun fiber membrane of the slowly-releasing anti-herpesvirus medicinal of gained of the present invention can realize the slowly-releasing of antiherpetic thing, can be applied to the drug release characteristics research of medical drug-loading fibre and the immunotherapy targeted autoantibody of herpes-like virus.
Accompanying drawing explanation
Fig. 1 is the fiber surface stereoscan photograph of the polyacrylonitrile nano electrospun fiber membrane (polyacrylonitrile concentration is 18%, and the mass ratio of polyacrylonitrile and ACV is 9:1) of the slowly-releasing anti-herpesvirus medicinal obtained in embodiment 1;
Fig. 2 is the fiber surface stereoscan photograph of the polyacrylonitrile nano electrospun fiber membrane (polyacrylonitrile concentration is 20%, and the mass ratio of polyacrylonitrile and ACV is 4:1) of the slowly-releasing anti-herpesvirus medicinal obtained in embodiment 1;
Fig. 3 is polyacrylonitrile hollow fiber (polyacrylonitrile concentration is 22%, and the mass ratio of polyacrylonitrile and ACV is 10:1) the ACV In Vitro Dissolution curve in phosphate buffer (pH7.4) of the discharged anti-herpesvirus medicinal ACV obtained in embodiment 3.
Detailed description of the invention
The invention provides a kind of polyacrylonitrile nano electrospinning fibre membrane preparation method of slowly-releasing anti-herpesvirus medicinal, concrete steps are:
(1) ACV is dissolved in organic solvent, obtain ACV solution, dry polyacrylonitrile powder is added again in this ACV solution, dissolve, obtained electrostatic spinning liquid, wherein in electrospinning liquid, polyacrylonitrile concentration is 18 ~ 22%, and the mass ratio of polyacrylonitrile and ACV is 4-19:1;
(2) solution configured is poured in lyse reservoir, and holder is placed on micro-injection pump, adopt the injection needle scabbled as the capillary spraying thread, connect the positive pole of high voltage source, aluminium foil fiber accepts dull and stereotyped connection negative pole, opening power;
(3) carry out according to suitable electrospinning condition the polyacrylonitrile nano electrospun fiber membrane that electrostatic spinning obtains slowly-releasing anti-herpesvirus medicinal.
Organic solvent selected by step (1) is dimethyl formamide DMF or methyl-sulfoxide DMSO or dimethylacetylamide DMAc, lyse reservoir in step (2) is 5 milliliters of injector to inject device syringe needles is No. 9 syringe needles, in step (3), technological parameter is: voltage 12-16 kilovolt, syringe fltting speed is 0.8-1.2 ml/hour, receiving range is 120-200 millimeter, and the area of the polyacrylonitrile nano electrospun fiber membrane of slowly-releasing anti-herpesvirus medicinal prepared in step (3) is 10cm*10cm.
As shown in Figure 1, effects on surface carries out metal spraying process under vacuo, by electron scanning electron microscopic observation fiber surface form, drug-loading fibre pattern is comparatively even, average diameter is 120 ± 27nm, is the fiber in nanometer range, has the many advantages that nanofiber possesses.
As shown in Figure 2, the medicament-carrying nano-fiber prepared under different condition has good pattern equally, average diameter is 160 ± 43nm, the raising of visible polymer concentration and the raising of drug concentration make fibre diameter increase, and also can be observed fiber surface has larger ACV medicine crystal particle aggregation simultaneously.
Fig. 3 illustrates that this medicine carrying superfine fibre can control medicine and progressively discharge more than 10 hours.With, the initial stage burst effect of medicine is comparatively obvious, and the initial stage, the prominent amount of releasing was 38.5%; After 36 hours, drug accumulation release is 62.3%.Illustrate that this medicament-carrying nano-fiber has obvious slow release effect.
Below by specific embodiment, technical scheme of the present invention is further described:
Embodiment 1
Dry polyacrylonitrile 0.9 gram is taken stand-by with electronic balance.Take 0.1 gram of ACV again to add in 5 milliliters of DMAc and dissolve completely, obtain the DMAc solution of ACV.Then polyacrylonitrile after drying is added mechanical agitation in the DMAc solution of ACV, until polyacrylonitrile powder dissolves completely, obtained electrospinning liquid.The solution configured is poured in 5 milliliters of syringes, adopt No. 9 injection needles scabbled as the capillary spraying thread, connect the positive pole of high voltage source, aluminium foil fiber accepts dull and stereotyped connection negative pole, opening power, regulation voltage is 12 kilovolts, and syringe fltting speed is 0.8 ml/hour, receiving range is 120 millimeters, and aluminium-foil paper receives and obtains the polyacrylonitrile nano electrospun fiber membrane that size is the slowly-releasing anti-herpesvirus medicinal of 10 centimetres x10 centimetre.
Embodiment 2
Dry polyacrylonitrile 1.0 grams is taken stand-by with electronic balance.Take 0.25 gram of ACV again to add in 5 milliliters of DMF and dissolve completely, obtain the DMF solution of ACV.Then polyacrylonitrile after drying is added mechanical agitation in the DMF solution of ACV, until polyacrylonitrile powder dissolves completely, obtained electrospinning liquid.The solution configured is poured in 5 milliliters of syringes, adopt No. 9 injection needles scabbled as the capillary spraying thread, connect the positive pole of high voltage source, aluminium foil fiber accepts dull and stereotyped connection negative pole, opening power, regulation voltage is 14 kilovolts, and syringe fltting speed is 1.0 mls/hour, receiving range is 140 millimeters, and aluminium-foil paper receives and obtains the polyacrylonitrile nano electrospun fiber membrane that size is the slowly-releasing anti-herpesvirus medicinal of 10 centimetres x10 centimetre.
Embodiment 3
Dry polyacrylonitrile 1.1 grams is taken stand-by with electronic balance.Take 0.11 gram of ACV again to add in 5 milliliters of DMSO and dissolve completely, obtain the DMSO solution of ACV.Then polyacrylonitrile after drying is added mechanical agitation in the DMSO solution of ACV, until polyacrylonitrile powder dissolves completely, obtained electrospinning liquid.The solution configured is poured in 5 milliliters of syringes, adopt No. 9 injection needles scabbled as the capillary spraying thread, connect the positive pole of high voltage source, aluminium foil fiber accepts dull and stereotyped connection negative pole, opening power, regulation voltage is 16 kilovolts, and syringe fltting speed is 1.2 mls/hour, receiving range is 160 millimeters, and aluminium-foil paper receives and obtains the polyacrylonitrile nano electrospun fiber membrane that size is the slowly-releasing anti-herpesvirus medicinal of 10 centimetres x10 centimetre.
Finally, it is also to be noted that what enumerate above is only several specific embodiment of the present invention; obviously, the invention is not restricted to above embodiment, many distortion can also be had; as long as all distortion that disclosed content directly derives or associates, protection scope of the present invention all should be thought.
Claims (6)
1. a polyacrylonitrile nano electrospinning fibre membrane preparation method for slowly-releasing anti-herpesvirus medicinal, is characterized in that, described anti-herpesvirus medicinal is ACV, and described concrete preparation process is as follows:
(1) ACV is dissolved in organic solvent, obtain ACV solution, dry polyacrylonitrile powder is added again in this ACV solution, dissolve, obtained electrostatic spinning liquid, wherein in electrospinning liquid, polyacrylonitrile concentration is 18 ~ 22%, and the mass ratio of polyacrylonitrile and ACV is 4-19:1;
(2) solution configured is poured in lyse reservoir, and holder is placed on micro-injection pump, adopt the injection needle scabbled as the capillary spraying thread, connect the positive pole of high voltage source, aluminium foil fiber accepts dull and stereotyped connection negative pole, opening power;
(3) carry out according to suitable electrospinning condition the polyacrylonitrile nano electrospun fiber membrane that electrostatic spinning obtains slowly-releasing anti-herpesvirus medicinal.
2. the polyacrylonitrile nano electrospinning fibre membrane preparation method of slowly-releasing anti-herpesvirus medicinal according to claim 1, it is characterized in that, the organic solvent selected by described step (1) is dimethyl formamide DMF or methyl-sulfoxide DMSO or dimethylacetylamide DMAc.
3. the polyacrylonitrile nano electrospinning fibre membrane preparation method of slowly-releasing anti-herpesvirus medicinal according to claim 1, is characterized in that, the lyse reservoir in described step (2) is 5 milliliters of syringes, and syringe needle is No. 9 syringe needles.
4. the polyacrylonitrile nano electrospinning fibre membrane preparation method of the slowly-releasing anti-herpesvirus medicinal according to claim 1 or 2 or 3, it is characterized in that, in described step (3), technological parameter is: voltage 12-16 kilovolt, syringe fltting speed is 0.8-1.2 ml/hour, and receiving range is 120-200 millimeter.
5. the polyacrylonitrile nano electrospinning fibre membrane preparation method of slowly-releasing anti-herpesvirus medicinal according to claim 4, is characterized in that: the area of the polyacrylonitrile nano electrospun fiber membrane of slowly-releasing anti-herpesvirus medicinal prepared in described step (3) is 10cm*10cm.
6. according to claim 1 or 2 or or the polyacrylonitrile nano electrospinning fibre membrane preparation method of slowly-releasing anti-herpesvirus medicinal described in 3 or 5, it is characterized in that: the area of the polyacrylonitrile nano electrospun fiber membrane of slowly-releasing anti-herpesvirus medicinal prepared in described step (3) is 10cm*10cm.
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| CN105944111A (en) * | 2016-04-13 | 2016-09-21 | 浪莎针织有限公司 | Preparation method of modified drug-loading PAN nanometer fiber mat |
| CN106319668A (en) * | 2016-09-08 | 2017-01-11 | 山东省医学科学院药物研究所 | Preparation method of high-yield core-shell drug-loading PAN nanofiber |
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Cited By (3)
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| CN106319668A (en) * | 2016-09-08 | 2017-01-11 | 山东省医学科学院药物研究所 | Preparation method of high-yield core-shell drug-loading PAN nanofiber |
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