CN105039481A - Rice bran polypeptide preparation method for improving polypeptide quality - Google Patents
Rice bran polypeptide preparation method for improving polypeptide quality Download PDFInfo
- Publication number
- CN105039481A CN105039481A CN201510467149.5A CN201510467149A CN105039481A CN 105039481 A CN105039481 A CN 105039481A CN 201510467149 A CN201510467149 A CN 201510467149A CN 105039481 A CN105039481 A CN 105039481A
- Authority
- CN
- China
- Prior art keywords
- rice bran
- polypeptide
- preparation
- enzyme
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a rice bran polypeptide preparation method for improving polypeptide quality. The rice bran polypeptide preparation method for improving the polypeptide quality comprises the following steps that (1) enzyme treatment is performed, namely defatted rice bran is taken, smashed and screened, then 8-12 times by weight of water is added to regulate a pH value to be 3.0-4.0, and alpha-amylase and cellulase are added to perform hydrolysis; (2) alkali diluting treatment is performed, a diluted NaOH solution is added into the material in the step (1) to regulate the pH value to be 8.5-9.5, then stirring is performed at the temperature of 45-60 DEG C for 30-60 minutes; (3) extra-high pressure treatment is performed, the material processed in the step (2) is processed by adopting an extra-high pressure technology under the pressure of 100-200 MPa, and the processing time is 3-15 minutes; (4) trypsin hydrolysis is performed, namely the pH value of the material processed in the step (3) is regulated to be 8.0-8.5, and then the trypsin is added to perform enzymolysis for hydrolysis; (5) enzyme deactivation and drying are performed, namely enzyme deactivation is conducted on the material processed in the step (4), the material is cooled to be room temperature, and then centrifugation, supernate collection, concentration and vacuum freeze drying are performed to obtain safe rice bran polypeptide. The purity and yield of polypeptide products are high, aflatoxin can be further efficiently removed, and the polypeptide quality is greatly improved.
Description
Technical field
The present invention relates to Rice Bran Polypeptides preparation method, be specifically related to a kind of preparation method improving the Rice Bran Polypeptides of polypeptide quality.
Background technology
Biologically active peptides is the general name of the different peptide classes of linear, the ring structure from dipeptides to complexity that in protein, 25 natural amino acids are formed with difference composition and arrangement mode, is the multi-functional compounds coming from protein.Bioactive peptide has multiple body metabolism and physiological regulation function, absorption easy to digest, there is the effect such as Promote immunity, hormone regulation, antibacterial, antiviral, hypotensive, reducing blood-fat, edible safety is high, be the research topic that current international food circle is the most popular and the functional factor having development prospect, have broad application prospects.
Rice bran is the principal by product in Rice producing, is the mixture that brown rice is processed into the cortex under grinding in polished rice process and a small amount of rice embryo and cracks rice, containing abundant rice bran protein.Rice Bran Polypeptides is the polypeptide mixture of the different molecular weight that rice bran obtains through protease hydrolysis, it is nutritious, absorption easy to digest, safety non-toxic, has found that rice bran peptide has the physiologically actives such as anti-oxidant, hypotensive, decreasing cholesterol, hypoglycemic, anticancer, antifatigue at present.Therefore, be that Rice Bran Polypeptides prepared by raw material with rice bran, the added value improving rice bran is had important practical significance.
Rice in field, the link such as storage and processing easily infects flavus or Aspergillus parasiticus, causes the AFB in the principal by product rice bran in Rice producing
1content overproof, finally cause being that the aflatoxin residual quantity of the Rice Bran Polypeptides that raw material obtains is higher with rice bran, do not reach the requirement (≤10 μ g/kg) of national standard far away, therefore, prepare in the process of Rice Bran Polypeptides and be necessary to take measures to remove aflatoxin, produce safe Rice Bran Polypeptides.
Aflatoxin (Aflatoxin, AF) be by the extremely strong secondary metabolite of the toxicity of the mould generations such as flavus (Aspergillusflavus), Aspergillus parasiticus (A.parasiticus), it is the derivative of the dihydrofuran tonka bean camphor of cluster structural similitude, its toxicity is 10 times of potassium cyanide, 68 times of arsenic, strong destruction is had to humans and animals liver organization, liver cancer can be caused time serious even dead, within 1993, being decided to be I class carcinogens by the Agency for Research on Cancer of WHO, is endanger one of maximum mycotoxins to human and animal.Current discovery in food crop with AFB
1, B
2, G
1, G
2the most common, wherein AFB
1(AFB
1) toxicity is the strongest.Aflatoxin contains large ring conjugated system, has good thermostability, decomposes up to just starting when 268 DEG C in envrionment temperature.
For removing of aflatoxin in rice and goods thereof, be mainly divided into Physical, chemical method and biological process at present.Physical comprises sieve method, irradiation method, heating method, absorption method etc., and chemical method comprises alkaline process, oxidation style etc.A kind of degradation method of aflatoxin disclosed in the Chinese patent application of publication number CN101238866A is the method adopting ultraviolet, microwave and light wave comprehensive treating process to remove aflatoxin, first with the aflatoxin on light wave auxiliary UV line degraded material surface, again by the aflatoxin of microwave action degraded internal batch, reach the object of the aflatoxin from outward appearance to inner essence removed in material.The disclosed method removing aflatoxin degradation disclosed in the method for aflatoxin and the Chinese patent application of publication number CN101491310A of Chinese patent application of publication number CN101731494A adopts ultraviolet and gamma-rays to remove aflatoxin respectively.Aforesaid method is not high to the decreasing ratio of aflatoxin, and there is destruction in various degree and impact to the local flavor of end product and quality, and correlation technique is difficult to reach industrialized level.
Traditional polypeptides matter preparation adopts enzymolysis process more, and first employing " alkali extraction and acid precipitation " technique extracts protein from rice bran usually, then to be hydrolyzed to protein with proteolytic enzyme and to prepare polypeptide.The method with aflatoxin contamination rice bran for extract raw material time, degradation rate only reaches 40.38 ~ 48.69%, AFB in finished product
1residual quantity is higher, at 10 more than μ g/kg, does not reach national standard far away.In extraction protein technique, the high pH that " alkali is molten " adopts causes protein denaturation even to produce toxic substance, " acid is heavy " brings too much inorganic salt into and desalination can cause protein losses, make the purity of Rice Bran Polypeptides and yield all not high, purity is 77.34%, yield is 56.16%, and not only product quality is not good enough, and cost is high.
Summary of the invention
The object of the present invention is to provide a kind of preparation method improving the Rice Bran Polypeptides of polypeptide quality, the method is by stepwise discretization and in conjunction with ultra-high voltage and diluted alkaline process, not only the purity of polypeptide products and yield high, can also efficient removal aflatoxin, greatly improve polypeptide quality.
Object of the present invention is achieved through the following technical solutions: a kind of preparation method improving the Rice Bran Polypeptides of polypeptide quality, comprises the following steps:
(1) ferment treatment: extracting degreasing rice bran, adds the water of 8 ~ 12 times of weight after pulverizing and sieving, adjust ph is 3.0 ~ 4.0, adds α-amylase and cellulase is hydrolyzed, with except the impurity such as destarching and fiber;
(2) diluted alkaline process: add dilute NaOH solution in the material of step (1), pH value is adjusted to 8.5 ~ 9.5, then stirs 30 ~ 60min at 45 ~ 60 DEG C;
(3) uhp treatment: superhighpressure technology process is carried out, pressure 100 ~ 200MPa, treatment time 3 ~ 15min to the material after step (2) process;
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed is adjusted to 8.0 ~ 8.5, then adds trypsin digestion and is hydrolyzed;
(5) enzyme is gone out with dry: the material after described step (4) process goes out enzyme, after being cooled to room temperature, centrifugal, collects supernatant liquor, concentrated, obtained safe Rice Bran Polypeptides after vacuum lyophilization.
Stepwise discretization of the present invention in conjunction with ultra-high voltage and diluted alkaline process, weak base improves the solvability of protein and not easily makes it sex change generation objectionable impurities on the one hand, improve the solubility rate of protein, under ultra-high voltage effect, accelerate stripping and the release of protein on the other hand, substantially increase Rice Bran Polypeptides purity and yield.When mouldy rice bran will be processed, because aflatoxin is unstable to alkali, weak base can be hydrolyzed rapidly aflatoxin in rice bran, and reaction generates ortho position tonka bean camphor sodium salt that can be water-soluble, only need wash and just can remove aflatoxin easily, effectively reduce the content of aflatoxin.Superhighpressure technology process then facilitates the dispersion of rice bran aggregation, is conducive to aflatoxin and alkali effect is degraded, and the aflatoxin content greatly in reduction Rice Bran Polypeptides, makes it to meet national quality safety standard requirement.
In described step (1), employing concentration is the salt acid for adjusting pH value of 0.5 ~ 1.2mol/L.
The addition of the α-amylase of described step (1) is add 120 ~ 180U in every grammeter chaff, and the addition of described cellulase is add 380 ~ 520U in every grammeter chaff, ferment treatment temperature 40 ~ 60 DEG C, enzyme processing time 30 ~ 55min.
In described step (2), the concentration of described dilute NaOH solution is 0.5 ~ 1.2mol/L.
In described step (4), with the throw out in material for mete-wand, described tryptic total addition level is add 5300 ~ 5900U in every gram of throw out.The described trypsin digestion time: 150 ~ 240min, temperature: 35 ~ 55 DEG C.
In described step (5), enzyme-removal temperature 85 ~ 95 DEG C, go out enzyme time 10 ~ 15min; Centrifugal rotating speed is 4000-5000r/min, time 4-5min.Described supernatant liquor is placed in dialysis tubing distilled water flushing 3-5 time, and then concentrates.
compared with prior art, the present invention has following beneficial effect:
(1) compare with traditional extraction method, the polypeptide rate of recovery of the present invention and purity high, solubleness improves, and indices (except foaming stability and retentiveness decline to some extent) is all significantly improved, and AFB
1,extrusion rate is up to 99.24%, AFB
1,residual quantity is far below the limit standard 10 μ g/kg of national regulation, and the quality of product effectively promotes and improves, for rice bran higher value application provides technical director.
(2), in Rice Bran Polypeptides preparation process, because rice bran protein exists the aggregate of a large amount of disulfide linkage and albumen and lipid, glucide, therefore most of protein solubility is poor, is difficult to extract.To this, the present invention extracts protein with weak base, and be aided with superhighpressure technology process, there occurs crosslinked under autoclaving effect between protein molecular, small molecules is gathered into macromole, and cross-linking part concentrates on hydrophobic grouping, improve rice bran protein matter wetting ability, solvability improves 3.24 ~ 5.62%, improves the solubility rate of rice bran protein matter, and the Rice Bran Polypeptides yield of gained is improved.
(3) compare with traditional enzyme process polypeptide production methods, the present invention adopts enzyme process to carry out substep and is separated, before preparing rice bran end product Rice Bran Polypeptides, adopt α-amylase and cellulase effectively can improve the efficiency of the direct enzymolysis Rice Bran Polypeptides of trypsinase simultaneously, make the purity of Rice Bran Polypeptides and yield improve 9.02 ~ 11.62% and 26.43 ~ 28.73% respectively.
(4) the present invention is at weak basic condition, reacts under being aided with ultra-high voltage environment, is conducive to avoiding strong basicity bringing into denaturation of protein and a large amount of sodium salt ion, make Rice Bran Polypeptides purity and utilization ratio higher.(5) the present invention utilizes weak base alkali refining to remove aflatoxin in conjunction with superhighpressure technology process, and wherein weak base makes aflatoxin be hydrolyzed, and reaction generates water-soluble ortho position tonka bean camphor sodium salt.And superhighpressure technology process facilitates the dispersion of rice bran aggregation, increase the contact area of alkali lye and aflatoxin, be conducive to aflatoxin and alkali effect, promote that aflatoxin is degraded to ortho position tonka bean camphor sodium salt, improve the degradation rate of aflatoxin, reach more than 91.63%, higher than 40.38 ~ 48.69% of traditional technology, make the aflatoxin residual quantity≤10 μ g/kg in Rice Bran Polypeptides, meet national quality safety standards.
Accompanying drawing explanation
Fig. 1 is the process flow sheet of following examples of the present invention.
Embodiment
embodiment 1
(1) ferment treatment: the mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, the water of 8 times of weight is added after pulverizing and sieving, be 3.0 ~ 4.0 with 0.5mol/L salt acid for adjusting pH, add α-amylase and cellulase, wherein, the addition of α-amylase is add 120U in every grammeter chaff, and the addition of cellulase is add 380U in every grammeter chaff, 30min is processed, except the impurity such as destarching and fiber at 40 DEG C.
(2) diluted alkaline process: the material toward step (1) gained adds 0.5mol/L dilute NaOH solution, pH value is adjusted to 8.5 ~ 9.5, then stirs 30min under 45 DEG C of water-baths.
(3) uhp treatment: superhighpressure technology process is carried out to the material processed through step (2), pressure 100MPa, treatment time 3min.
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed with 0.5mol/L hydrochloric acid is adjusted to 8.0, and then add trypsinase, its total addition level is add 5300U in every gram of throw out, enzymolysis 150min under 35 DEG C of water-baths.
(5) enzyme is gone out with dry: 90 DEG C of enzyme 10min that go out, are cooled to room temperature, and then the centrifugal 5min of 4000r/min gets supernatant liquor, be placed in dialysis tubing distilled water flushing 3-5 time, then concentrate, vacuum lyophilization, the Rice Bran Polypeptides of obtained safety, wherein rice bran AFB
1degradation rate is 91.63%, and residual quantity is 8.37 μ g/kg, Purity 86.36%, yield 82.59%, the rate of recovery 71.32%, degree of hydrolysis DH is 8.68%, solubleness 1.35%, emulsifying property 0.34, emulsifying stability 39.28min, whipability 36.33%, foaming stability 23.25%, absorptivity 5.97g, oil absorbency 1.82g.
embodiment 2
(1) ferment treatment: the mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, the water of 8.5 times of weight is added after pulverizing and sieving, be 3.0 ~ 4.0 with 0.5mol/L salt acid for adjusting pH, add α-amylase and cellulase, wherein, the addition of α-amylase is add 125U in every grammeter chaff, and the addition of cellulase is add 390U in every grammeter chaff, 35min is processed, except the impurity such as destarching and fiber at 45 DEG C.
(2) diluted alkaline process: the material toward step (1) gained adds 0.8mol/L dilute NaOH solution, pH value is adjusted to 8.5 ~ 9.5, then stirs 40min under 45 DEG C of water-baths.
(3) uhp treatment: superhighpressure technology process is carried out to the material processed through step (2), pressure 120MPa, treatment time 5min.
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed with 0.5mol/L hydrochloric acid is adjusted to 8.2, and then add trypsinase, its total addition level is add 5400U in every gram of throw out, enzymolysis 160min under 40 DEG C of water-baths.
(5) enzyme is gone out with dry: 90 DEG C of enzyme 10min that go out, are cooled to room temperature, and then the centrifugal 5min of 4000r/min gets supernatant liquor, be placed in dialysis tubing distilled water flushing 3-5 time, then concentrate, vacuum lyophilization, the Rice Bran Polypeptides of obtained safety, wherein rice bran AFB
1degradation rate is 93.27%, and residual quantity is 6.73 μ g/kg, Purity 86.89%, yield 83.06%, the rate of recovery 72.17%, degree of hydrolysis DH is, 8.95%, solubleness 2.72%, emulsifying property 0.36, emulsifying stability 41.53min, whipability 45.29%, foaming stability 20.17%, absorptivity 5.12g, oil absorbency 2.25g.
embodiment 3
(1) ferment treatment: the mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, the water of 9 times of weight is added after pulverizing and sieving, be 3.0 ~ 4.0 with 0.5mol/L salt acid for adjusting pH, add α-amylase and cellulase, wherein, the addition of α-amylase is add 135U in every grammeter chaff, and the addition of cellulase is add 400U in every grammeter chaff, 45min is processed, except the impurity such as destarching and fiber at 45 DEG C.
(2) diluted alkaline process: the material toward step (1) gained adds 1.0mol/L dilute NaOH solution, pH value is adjusted to 8.5 ~ 9.5, then stirs 45min under 45 DEG C of water-baths.
(3) uhp treatment: though the material after step (2) process carries out superhighpressure technology process, pressure 140MPa, treatment time 7min.
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed with 0.5mol/L hydrochloric acid is adjusted to 8.2, and then add trypsinase, its total addition level is add 5600U in every gram of throw out, enzymolysis 180min under 50 DEG C of water-baths.
(5) enzyme is gone out with dry: 90 DEG C of enzyme 10min that go out, are cooled to room temperature, and then the centrifugal 5min of 4000r/min gets supernatant liquor, be placed in dialysis tubing distilled water flushing 3-5 time, then concentrate, vacuum lyophilization, the Rice Bran Polypeptides of obtained safety, wherein rice bran AFB
1degradation rate is 95.18%, and residual quantity is 4.82 μ g/kg, Purity 87.06%, yield 83.28%, the rate of recovery 72.50%, degree of hydrolysis DH is 9.32%, solubleness 3.38%, emulsifying property 0.42, emulsifying stability 43.38min, whipability 48.62%, foaming stability 18.49%, absorptivity 4.86g, oil absorbency 2.56g.
embodiment 4
(1) ferment treatment: the mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, the water of 9.5 times of weight is added after pulverizing and sieving, be 3.0 ~ 4.0 with 0.5mol/L salt acid for adjusting pH, add α-amylase and cellulase, wherein, the addition of α-amylase is add 140U in every grammeter chaff, and the addition of cellulase is add 420U in every grammeter chaff, 50min is processed, except the impurity such as destarching and fiber at 50 DEG C.
(2) diluted alkaline process: the material toward step (1) gained adds 1.2mol/L dilute NaOH solution, pH value is adjusted to 8.5 ~ 9.5, then stirs 45min under 50 DEG C of water-baths.
(3) uhp treatment: superhighpressure technology process is carried out, pressure 160MPa, treatment time 7min to the material after step (2) process.
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed with 0.5mol/L hydrochloric acid is adjusted to 8.4, and then add trypsinase, its total addition level is add 5800U in every gram of throw out, enzymolysis 200min under 50 DEG C of water-baths.
(5) enzyme is gone out with dry: 90 DEG C of enzyme 10min that go out, are cooled to room temperature, and then the centrifugal 5min of 4000r/min gets supernatant liquor, be placed in dialysis tubing distilled water flushing 3-5 time, then concentrate, vacuum lyophilization, the Rice Bran Polypeptides of obtained safety, wherein rice bran AFB
1degradation rate is 97.16%, and residual quantity is 2.84 μ g/kg, Purity 87.26%, yield 84.15%, the rate of recovery 73.43%, degree of hydrolysis DH is 9.78%, solubleness 4.31%, emulsifying property 0.46, emulsifying stability 45.73min, whipability 51.31%, foaming stability 15.13%, absorptivity 4.64g, oil absorbency 2.85g.
embodiment 5
(1) ferment treatment: the mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, the water of 9.5 times of weight is added after pulverizing and sieving, be 3.0 ~ 4.0 with 0.5mol/L salt acid for adjusting pH, add α-amylase and cellulase, wherein, the addition of α-amylase is add 145U in every grammeter chaff, and the addition of cellulase is add 440U in every grammeter chaff, 50min is processed, except the impurity such as destarching and fiber at 45 DEG C.
(2) diluted alkaline process: the material toward step (1) gained adds 1.2mol/L dilute NaOH solution, pH value is adjusted to 8.5 ~ 9.5, then stirs 50min under 50 DEG C of water-baths.
(3) uhp treatment: superhighpressure technology process is carried out, pressure 180MPa, treatment time 7min to the material after step (2) process.
(4) trypsin hydrolyzing: be adjusted to 8.2 by the pH value after step (3) processes by 0.5mol/L hydrochloric acid, then add trypsinase, its total addition level is add 5900U in every gram of throw out, enzymolysis 220min under 55 DEG C of water-baths.
(5) enzyme is gone out with dry: 90 DEG C of enzyme 10min that go out, are cooled to room temperature, and then the centrifugal 5min of 4000r/min gets supernatant liquor, be placed in dialysis tubing distilled water flushing 3-5 time, then concentrate, vacuum lyophilization, the Rice Bran Polypeptides of obtained safety, wherein rice bran AFB
1degradation rate is 98.86%, and residual quantity is 1.14 μ g/kg, Purity 87.66%, yield 84.35%, the rate of recovery 73.94%, degree of hydrolysis DH is 10.56%, solubleness 4.56%, emulsifying property 0.52, emulsifying stability 47.22min, whipability 55.47%, foaming stability 13.94%, absorptivity 4.26g, oil absorbency 3.04g.
embodiment 6
(1) ferment treatment: the mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, the water of 12 times of weight is added after pulverizing and sieving, be 3.0 ~ 4.0 with 0.5mol/L salt acid for adjusting pH, add α-amylase and cellulase, wherein, the addition of α-amylase is add 160U in every grammeter chaff, and the addition of cellulase is add 450U in every grammeter chaff, 60min is processed, except the impurity such as destarching and fiber at 50 DEG C.
(2) diluted alkaline process: the material toward step (1) gained adds 1.2mol/L dilute NaOH solution, pH value is adjusted to 8.5 ~ 9.5, then stirs 50min under 60 DEG C of water-baths.
(3) uhp treatment: superhighpressure technology process is carried out, pressure 180MPa, treatment time 9min to the material after step (2) process.
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed with 0.5mol/L hydrochloric acid is adjusted to 8.2, and then add trypsinase, its total addition level is add 5900U in every gram of throw out, enzymolysis 240min under 55 DEG C of water-baths.
(5) enzyme is gone out with dry: 90 DEG C of enzyme 10min that go out, are cooled to room temperature, and then the centrifugal 5min of 4000r/min gets supernatant liquor, be placed in dialysis tubing distilled water flushing 3-5 time, then concentrate, vacuum lyophilization, the Rice Bran Polypeptides of obtained safety, wherein rice bran AFB
1degradation rate is 98.93%, and residual quantity is 1.07 μ g/kg, Purity 87.71%, yield 84.64%, the rate of recovery 74.23%, degree of hydrolysis DH is 10.74%, solubleness 4.68%, emulsifying property 0.56, emulsifying stability 48.53min, whipability 59.82%, foaming stability 11.26%, absorptivity 4.13g, oil absorbency 3.15g.
embodiment 7
(1) ferment treatment: the mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, the water of 10 times of weight is added after pulverizing and sieving, be 3.0 ~ 4.0 with 0.5mol/L salt acid for adjusting pH, add α-amylase and cellulase, wherein, the addition of α-amylase is add 160U in every grammeter chaff, and the addition of cellulase is add 500U in every grammeter chaff, 60min is processed, except the impurity such as destarching and fiber at 55 DEG C.
(2) diluted alkaline process: the material toward step (1) gained adds 1.2mol/L dilute NaOH solution, pH value is adjusted to 8.5 ~ 9.5, then stirs 50min under 55 DEG C of water-baths.
(3) uhp treatment: superhighpressure technology process is carried out, pressure 200MPa, treatment time 15min to the material after step (2) process.
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed with 0.5mol/L hydrochloric acid is adjusted to 8.5, and then add trypsinase, its total addition level is add 5900U in every gram of throw out, enzymolysis 240min under 55 DEG C of water-baths.
(5) enzyme is gone out with dry: 90 DEG C of enzyme 10min that go out, are cooled to room temperature, and then the centrifugal 5min of 4000r/min gets supernatant liquor, be placed in dialysis tubing distilled water flushing 3-5 time, then concentrate, vacuum lyophilization, the Rice Bran Polypeptides of obtained safety, wherein rice bran AFB
1degradation rate is 99.24%, and residual quantity is 0.76 μ g/kg, Purity 88.96%, yield 84.89%, the rate of recovery 75.52%, degree of hydrolysis DH is 11.33%, solubleness 4.87%, emulsifying property 0.58, emulsifying stability 49.36min, whipability 63.25%, foaming stability 9.33%, absorptivity 4.09g, oil absorbency 3.26g.
comparative example 1:
Concrete operation method is see such as Publication about Document: Wei Ming, Xue Zhenglian, money gloomy and. enzyme process prepares the research of Rice Bran Polypeptides. Chinese oil, 2014,39 (9): 27-30..
The mouldy rice bran of extracting degreasing, AFB
1content 100 μ g/kg, with reference to aforesaid method, under being chosen at optimum process condition, soak by 1:20 solid-liquid ratio, 45 DEG C of ultrasonic agitation lixiviate 3h, enzyme concentration 1.5% hydrolysis temperature 40.5 DEG C of papoid, pH5.6, enzymolysis time 3.4h, go out enzyme, centrifuging and taking supernatant liquor, obtains Rice Bran Polypeptides product, wherein rice bran AFB
1degradation rate is 46.31%, and residual quantity is 53.69 μ g/kg, Purity 81.23%, yield 79.30%, the rate of recovery 66.41%, degree of hydrolysis DH is 8.54%, solubleness 0.75%, emulsifying property 0.36, emulsifying stability 32.12min, whipability 16.68%, foaming stability 24.62%, absorptivity 6.13g, oil absorbency 1.69g.
The present invention can summarize with other the specific form without prejudice to spirit of the present invention or principal character.Above-mentioned embodiment of the present invention all can only be thought explanation of the present invention instead of restriction, therefore every above embodiment is done according to substantial technological of the present invention any trickle amendment, equivalent variations and modification, all belong in the scope of technical solution of the present invention.
Claims (8)
1. improve a preparation method for the Rice Bran Polypeptides of polypeptide quality, it is characterized in that, comprise the following steps:
(1) ferment treatment: extracting degreasing rice bran, adds the water of 8 ~ 12 times of weight after pulverizing and sieving, adjust ph is 3.0 ~ 4.0, adds α-amylase and cellulase is hydrolyzed;
(2) diluted alkaline process: add dilute NaOH solution in the material of step (1), pH value is adjusted to 8.5 ~ 9.5, then stirs 30 ~ 60min at 45 ~ 60 DEG C;
(3) uhp treatment: superhighpressure technology process is carried out, pressure 100 ~ 200MPa, treatment time 3 ~ 15min to the material after step (2) process;
(4) trypsin hydrolyzing: the pH value of the material after step (3) being processed is adjusted to 8.0 ~ 8.5, then adds trypsin digestion and is hydrolyzed;
(5) enzyme is gone out with dry: the material after described step (4) process goes out enzyme, after being cooled to room temperature, centrifugal, collects supernatant liquor, concentrated, obtained safe Rice Bran Polypeptides after vacuum lyophilization.
2. the preparation method of the Rice Bran Polypeptides of raising polypeptide quality according to claim 1, is characterized in that, in described step (1), employing concentration is the salt acid for adjusting pH value of 0.5 ~ 1.2mol/L.
3. the preparation method of the Rice Bran Polypeptides of raising polypeptide quality according to claim 1, it is characterized in that, the addition of the α-amylase of described step (1) is add 120 ~ 180U in every grammeter chaff, the addition of described cellulase is add 380 ~ 520U in every grammeter chaff, ferment treatment temperature is 40 ~ 60 DEG C, and enzyme processing time is 30 ~ 55min.
4. the preparation method of the Rice Bran Polypeptides of raising polypeptide quality according to claim 1, is characterized in that, in described step (2), the concentration of described dilute NaOH solution is 0.5 ~ 1.2mol/L.
5. the preparation method of the Rice Bran Polypeptides of the raising polypeptide quality according to claim 1 or 2 or 3 or 4, it is characterized in that, in described step (4), with the throw out in material for mete-wand, described tryptic total addition level is add 5300 ~ 5900U in every gram of throw out.
6. the preparation method of the Rice Bran Polypeptides of raising polypeptide quality according to claim 5, is characterized in that, described trypsin digestion time: 150 ~ 240min, temperature: 35 ~ 55 DEG C.
7. the preparation method of the Rice Bran Polypeptides of raising polypeptide quality according to claim 6, is characterized in that, in described step (5), and enzyme-removal temperature 85 ~ 95 DEG C, go out enzyme time 10 ~ 15min; Centrifugal rotating speed is 4000-5000r/min, time 4-5min.
8. the preparation method of the Rice Bran Polypeptides of raising polypeptide quality according to claim 7, is characterized in that, in described step (5), supernatant liquor is placed in dialysis tubing distilled water flushing 3-5 time, and then concentrates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510467149.5A CN105039481B (en) | 2015-08-03 | 2015-08-03 | A kind of preparation method for the Rice Bran Polypeptides for improving polypeptide quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510467149.5A CN105039481B (en) | 2015-08-03 | 2015-08-03 | A kind of preparation method for the Rice Bran Polypeptides for improving polypeptide quality |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105039481A true CN105039481A (en) | 2015-11-11 |
| CN105039481B CN105039481B (en) | 2018-05-22 |
Family
ID=54446449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510467149.5A Active CN105039481B (en) | 2015-08-03 | 2015-08-03 | A kind of preparation method for the Rice Bran Polypeptides for improving polypeptide quality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105039481B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106509333A (en) * | 2016-10-11 | 2017-03-22 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of high-solubility aflatoxins-removed rice bran protein |
| CN107468610A (en) * | 2017-08-16 | 2017-12-15 | 澳宝化妆品（惠州）有限公司 | A kind of extraction product of rice bran shell and its application in facial-care |
| CN111011712A (en) * | 2019-12-10 | 2020-04-17 | 中国农业科学院农产品加工研究所 | Fermented rice bran with intestinal probiotic activity and preparation method thereof |
| CN114150033A (en) * | 2021-12-06 | 2022-03-08 | 哈尔滨商业大学 | Preparation method of rice bran antioxidant peptide compound treated by peak alpha amylase |
| CN114395599A (en) * | 2022-01-05 | 2022-04-26 | 湖北省农业科学院农产品加工与核农技术研究所 | Preparation method of composite polypeptide |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434980A (en) * | 2008-12-08 | 2009-05-20 | 广东省农业科学院农业生物技术研究所 | Preparation of rice bran short peptide |
| CN102080118A (en) * | 2010-06-23 | 2011-06-01 | 东北农业大学 | Preparation method of rice bran antioxidant active protein peptide |
| CN102266004A (en) * | 2011-08-10 | 2011-12-07 | 江西金农生物科技有限公司 | Edible defatted rice bran and preparation method thereof |
| CN102524515A (en) * | 2012-02-21 | 2012-07-04 | 安徽农业大学 | Method for preparing high-purity rice protein from rice slag |
| CN103450256A (en) * | 2013-09-11 | 2013-12-18 | 无锡群硕谷唐生物科技有限公司 | Comprehensive utilization method of defatted rice bran |
| CN104404115A (en) * | 2014-11-11 | 2015-03-11 | 河南旭瑞饲料有限公司 | Production method of high purity enzymatically hydrolyzed rice protein powder |
-
2015
- 2015-08-03 CN CN201510467149.5A patent/CN105039481B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434980A (en) * | 2008-12-08 | 2009-05-20 | 广东省农业科学院农业生物技术研究所 | Preparation of rice bran short peptide |
| CN102080118A (en) * | 2010-06-23 | 2011-06-01 | 东北农业大学 | Preparation method of rice bran antioxidant active protein peptide |
| CN102266004A (en) * | 2011-08-10 | 2011-12-07 | 江西金农生物科技有限公司 | Edible defatted rice bran and preparation method thereof |
| CN102524515A (en) * | 2012-02-21 | 2012-07-04 | 安徽农业大学 | Method for preparing high-purity rice protein from rice slag |
| CN103450256A (en) * | 2013-09-11 | 2013-12-18 | 无锡群硕谷唐生物科技有限公司 | Comprehensive utilization method of defatted rice bran |
| CN104404115A (en) * | 2014-11-11 | 2015-03-11 | 河南旭瑞饲料有限公司 | Production method of high purity enzymatically hydrolyzed rice protein powder |
Non-Patent Citations (1)
| Title |
|---|
| 何东平: "脱脂米糠饼制备米糠多肽的研究", 《粮食与饲料工业》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106509333A (en) * | 2016-10-11 | 2017-03-22 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of high-solubility aflatoxins-removed rice bran protein |
| CN106509333B (en) * | 2016-10-11 | 2019-07-09 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of preparation method of the de- aflatoxin rice bran protein of highly dissoluble |
| CN107468610A (en) * | 2017-08-16 | 2017-12-15 | 澳宝化妆品（惠州）有限公司 | A kind of extraction product of rice bran shell and its application in facial-care |
| CN111011712A (en) * | 2019-12-10 | 2020-04-17 | 中国农业科学院农产品加工研究所 | Fermented rice bran with intestinal probiotic activity and preparation method thereof |
| CN111011712B (en) * | 2019-12-10 | 2022-05-27 | 中国农业科学院农产品加工研究所 | Fermented rice bran with intestinal probiotic activity and preparation method thereof |
| CN114150033A (en) * | 2021-12-06 | 2022-03-08 | 哈尔滨商业大学 | Preparation method of rice bran antioxidant peptide compound treated by peak alpha amylase |
| CN114150033B (en) * | 2021-12-06 | 2023-05-19 | 哈尔滨商业大学 | Preparation method of rice bran antioxidant peptide compound treated by peak alpha amylase |
| CN114395599A (en) * | 2022-01-05 | 2022-04-26 | 湖北省农业科学院农产品加工与核农技术研究所 | Preparation method of composite polypeptide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105039481B (en) | 2018-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105039481A (en) | Rice bran polypeptide preparation method for improving polypeptide quality | |
| CN102273543B (en) | Method for preparing peanut antioxidant peptide through ultrasonic-assisted enzymolysis | |
| CN1281628C (en) | Method for extracting edible tree fungi polysaccharide | |
| CN104004813B (en) | A kind of preparation of mushroom biologically active peptide | |
| CN100336832C (en) | Process for extracting comb shell polysaccharide | |
| CN103315129B (en) | Purely physical process method for extracting rice protein and starch | |
| CN103571609A (en) | Method for synchronously preparing high-stability rice bran oil and rice bran protein | |
| CN103976413B (en) | A kind of method extracting protein and dietary fiber from walnut dregs continuously | |
| CN111944866A (en) | Method for preparing yak hide small molecule collagen peptide by continuous rotary evaporation, desolventizing and double enzymolysis | |
| CN104687010B (en) | A kind of preparation method of less salt shrimp paste | |
| CN104207150A (en) | Oral liquid containing soybean peptides and fungi polysaccharides and preparation method thereof | |
| CN105779545A (en) | Method for preparing soft-shelled turtle protein source antioxidant peptide with microwave-assisted enzyme method | |
| CN101434980B (en) | Preparation of rice bran short peptide | |
| CN107307432A (en) | A kind of method that utilization needle mushroom pin produces food fibre powder | |
| CN112940145A (en) | Method for extracting and preparing animal ganoderma lucidum polysaccharide | |
| CN104531814B (en) | A kind of preparation method of Extracted From Oil-tea-cake enzyme modification albumen | |
| CN108517344A (en) | A kind of preparation method of ginseng peptide | |
| CN116035218A (en) | Pea oligopeptide health care product and preparation method thereof | |
| CN105567772B (en) | A kind of high antioxidant protein peptides and the preparation method and application thereof | |
| CN108967900A (en) | A kind of selenium-rich salubrity powder and preparation method thereof | |
| CN109645502A (en) | High-quality dietary fiber, the preparation method of honey raisin tree diet fiber composition and product | |
| WO2007041950A1 (en) | Extraction method of patinopecten yessoensis polysaccharide | |
| CN107893095A (en) | A kind of method that marine source albumen prepares high F value oligopeptide | |
| CN110144017B (en) | Continuous preparation process of active ingredients in bamboo fungus spores | |
| CN113100441A (en) | Method for preparing nanoparticles by combining soybean peptide aggregate and EGCG (epigallocatechin gallate) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20151111 Assignee: Guangzhou Baosangyuan Ecological Technology Co.,Ltd. Assignor: SERICULTURE & AGRI FOOD Research Institute GAAS Contract record no.: X2023440020007 Denomination of invention: A preparation method of rice bran polypeptide to improve the quality of polypeptide Granted publication date: 20180522 License type: Common License Record date: 20230221 |