CN105021761B - A kind of method of the content and its true and false of a variety of organic acids in detection grape juice - Google Patents
A kind of method of the content and its true and false of a variety of organic acids in detection grape juice Download PDFInfo
- Publication number
- CN105021761B CN105021761B CN201510387717.0A CN201510387717A CN105021761B CN 105021761 B CN105021761 B CN 105021761B CN 201510387717 A CN201510387717 A CN 201510387717A CN 105021761 B CN105021761 B CN 105021761B
- Authority
- CN
- China
- Prior art keywords
- msub
- acid
- grape juice
- mrow
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of while determining the method for a variety of organic acid contents in grape juice, it is to be detected after testing sample is extracted with high performance liquid chromatograph, the high performance liquid chromatograph contains DAD detectors, the DAD detectors set Detection wavelength as 210nm, the chromatographic column of the high performance liquid chromatograph is reversed-phase column, the ammonium dihydrogen phosphate cushioning liquid that mobile phase is methanol and pH is 2.8, the volume ratio of the methanol and ammonium dihydrogen phosphate cushioning liquid is 1: 99, the column temperature of the reversed-phase column is 30 DEG C, and flow velocity is 0.8mL/min.The invention also discloses a kind of grape juice authenticity identification method.The present invention is using that can measure the content of eight kinds of organic acids in grape juice simultaneously, and accuracy in detection is high, precision is good, error is small, and the true and false of grape juice differentiates accuracy up to more than 90%.
Description
Technical field
The invention belongs to analytical chemistry and food false distinguishing field, and in particular to the assay of a variety of organic acids in grape juice
Method and grape juice authenticity identification method.
Background technology
Grape nutritive value is very high, in addition to containing a variety of organic acids, mineral matter, vitamin and essential amino acid, also
Containing various bioactivators such as resveratrol, OPC, anthocyanidin, catechin and flavone compounds, with remove from
By base and antioxidation activity, cardioprotection, the different physiological roles such as immunity are improved, therefore, grape juice and grape juice in recent years
Product turns into the drink of prevalence in the world.In the case where the huge market demand is stimulated, the grape juice for mixing other fruit juice pretends to be pure
It is found during the phenomenon of grape juice, therefore, the true and false for how quick and precisely differentiating pure grape juice is consumer, the producer and quality inspection
The active demand of management organization.
Organic acid is the product of secondary metabolism during fruit growing, and it is constituted and content is that variety classes pure juice is intrinsic
Metric attribute, using the otherness of the species and content of organic acid in pure juice, pure juice Determination of Organic Acids can be set up
Finger-print, is analyzed and is judged to adulterated sample with reference to chemometrics method.At present to the detection method master of organic acid
Have including reversed-phase liquid chromatography (RP-HPLC) method, ion-exchange chromatography (IEC), Capillary Electrophoresis (HPCE) and gas-chromatography.
Wherein ion-exchange chromatography have the advantages that pre-treatment it is simple, while analyze a variety of organic acids, but occur analysis result sometimes
The problem of reappearance is unstable by method associated with multi-detector, it is necessary to be solved.Capillary Electrophoresis is present because small molecule has
Machine acid UV absorption is extremely low, and the sensitivity of Conventional UV detection (UV) is not high.And pre-treatment during gas Chromatographic Determination organic acid
Journey is more complicated.Reversed-phased high performace liquid chromatographic (RP-HPLC) have the advantages that quick, sensitive, applicability it is wide be widely deployed and
Use.Organic acid is soluble in water, preferably can be analyzed and be identified by RP-HPLC, at present the organic acid in fruit, fruit juice and fruit wine
RP-HPLC is widely used in detection.But the content of a variety of organic acids in grape juice is determined simultaneously, and identifies Portugal based on this
The method of the grape juice true and false is there is not yet document report.
The content of the invention
It is an object of the present invention to provide a kind of while determining the HPLC methods of a variety of organic acid contents in grape juice, originally
Another purpose of invention is to provide a kind of distinguishing method between true and false of grape juice.
Above-mentioned purpose is achieved through the following technical solutions：
It is a kind of at the same determine grape juice in a variety of organic acid contents method, it is that efficient liquid is used after testing sample is extracted
Chromatography detects the content of tartaric acid, malic acid, ascorbic acid, lactic acid, citric acid, acetic acid, fumaric acid, butanedioic acid simultaneously,
The high performance liquid chromatograph contains DAD detectors, and the DAD detectors set Detection wavelength as 210nm, the efficient liquid
The chromatographic column of chromatography is reversed-phase column, and mobile phase is the ammonium dihydrogen phosphate cushioning liquid that methanol and pH are 2.8, the methanol with
The volume ratio of ammonium dihydrogen phosphate cushioning liquid is 1: 99, and the column temperature of the reversed-phase column is 30 DEG C, and flow velocity is 0.8mL/min.
Preferably, it the described method comprises the following steps：
1) accurate weigh is dried to the tartaric acid of constant weight, malic acid, ascorbic acid, lactic acid, citric acid, acetic acid, fumaric acid
And each 10mg of butanedioic acid reference substance, 10mL is settled to after being dissolved with ultra-pure water, with 0.22 μm of filtering with microporous membrane, reference substance is used as
Solution；
2) testing sample 5mL, plus methanol and pH is taken to be diluted to 25mL, the first for 2.8 ammonium dihydrogen phosphate cushioning liquid
The volume ratio of alcohol and ammonium dihydrogen phosphate cushioning liquid is 1: 99, after ultrasound is mixed, and centrifugation takes supernatant 1mL through 0.22 μm of micropore
Membrane filtration, is used as sample solution；
3) chromatographic condition and system suitability：Chromatographic column is reversed-phase column, and stationary phase is polar embedded C18 bonded silica gels；First
The ammonium dihydrogen phosphate cushioning liquid that alcohol and pH are 2.8 is mobile phase, and the volume ratio of methanol and ammonium dihydrogen phosphate cushioning liquid is 1:
99；DAD detectors, Detection wavelength is 210nm；30 DEG C of column temperature；Flow velocity is 0.8mL/min；
4) accurate reference substance solution of drawing is detected with each 10 μ l of sample solution injection liquid chromatographs respectively.
A kind of grape juice authenticity identification method, it comprises the following steps：
1) content of a variety of organic acids in grape juice is determined according to the method described above；
2) seven kinds of organic acids (tartaric acid, malic acid, ascorbic acid, lactic acid, the lemons determined in true grape juice are selected
Acid, fumaric acid, butanedioic acid) content be modeling index, modeling index is handled using Logistic homing methods, will be true
The π values of grape juice are denoted as 1, and the π values of pseudo- grape juice are denoted as 0, can obtain the parameter fitting of Logistic models：
By step 1) seven kinds of organic acids (tartaric acid, malic acid, ascorbic acid, lactic acid, citric acid, fumaric acid, ambers measuring
Amber acid) content substitute into above formula, calculate π values, it 1 is true grape juice that π values, which are, π values are 0 to be pseudo- grape juice, formula, x1、x2、
x3、x4、x5、x6、x7The content of tartaric acid, malic acid, ascorbic acid, lactic acid, citric acid, fumaric acid, butanedioic acid is represented respectively, it is single
Position is mg/mL.
The beneficial effects of the invention are as follows：
1) present invention can measure eight kinds simultaneously using the content of organic acid in rp-hplc determination grape juice
The content of organic acid, accuracy in detection is high, precision is good, error is small, it is adaptable to the fast qualitative of organic acid and fixed in grape juice
Amount analysis.
2) present invention uses the content of seven kinds of organic acids for modeling index, is obtained using Logistic homing methods
The parameter fitting of Logistic models, by the model and the organic acid content measured, the true of grape juice can be identified exactly
Puppet, the accuracy that the true and false differentiates is up to more than 90%.
Brief description of the drawings
Fig. 1 is detected in collection of illustrative plates, figure for the HPLC of reference substance solution：1. tartaric acid；2. malic acid；3. ascorbic acid；4. breast
Acid；5. acetic acid；6. citric acid；7. fumaric acid；8. butanedioic acid.
Fig. 2 is detected in collection of illustrative plates, figure for the HPLC of sample solution：1. tartaric acid；2. malic acid；3. ascorbic acid；4. lactic acid；
5. citric acid；6. fumaric acid；7. butanedioic acid.
Embodiment
The present invention is described in detail with reference to embodiments.
Embodiment 1
1. the content of a variety of organic acids in grape juice is detected simultaneously
A, it is accurate weigh be dried under reduced pressure the tartaric acid to permanent quality, malic acid, ascorbic acid, lactic acid, acetic acid, citric acid,
Fumaric acid and each 10.0mg of butanedioic acid reference substance, constant volume is filtered in 10mL volumetric flasks with 0.22 μm of micropore after being dissolved with ultra-pure water
Membrane filtration.
B, grape juice sample 5mL plus mobile phase is taken to be diluted to 25mL, after ultrasound is mixed, centrifugation (9000rpm/min,
10min), supernatant 1mL is taken to obtain sample through 0.22 μm of filtering with microporous membrane.
C, sample made from a, b analyzed under the following conditions：Thermo Hypersil Gold aQ C18 posts (250 ×
4.6mm, 5 μm)；Mobile phase：Methanol: (2.8)=1: 99 (V/V) 0.02mol/L, pH are to ammonium dihydrogen phosphate cushioning liquid；Sample introduction
Amount：10μL；Flow velocity：0.8mL/min；Chromatogram column temperature：30℃；Detector：DAD；Detection wavelength：210nm.Washed using constant gradient
It is de-.
Peak area must be corresponded to, organic acid content is calculated by following equation：
Content (cx)=cr × Ax/Ar
In formula：
Cx---- sample concentrations mg/mL；
Cr---- control concentration mg/mL；
Ax---- sample peak areas；
Ar---- compares peak area.
The detection collection of illustrative plates of reference substance solution (containing eight kinds of organic acid standard items) is shown in Fig. 1.
The detection collection of illustrative plates of sample solution is shown in Fig. 2.
2. methodological study
2.1 regression analyses, detection limit.It the results are shown in Table 1.
The regression analysis of 18 kinds of organic acids of table, detection limit
2.2 the rate of recovery
Same two parts of pure grape juice sample is taken, a copy of it is as blank, and another adds a certain amount of standard organic acid
Mixed reference substance solution, it is determined that chromatographic condition under carry out RP-HPLC-DAD analyses, repeat 3 times, calculate mark-on reclaims
Rate and relative standard deviation (RSD), the results are shown in Table 2.
The rate of recovery, the relative standard deviation (n=3) of 28 kinds of organic acids of table
The rate of recovery of 8 kinds of organic acids is 95.60%-104.48%, and the rate of recovery is high, and relative standard deviation is respectively less than 5%,
Meet the requirement that organic acid compound is analyzed in grape juice sample.
2.3 precision
It is determined that liquid phase chromatogram condition under RP-HPLC analyses, parallel company are carried out to 8 kinds of organic acid mixed reference substance solutions
It is continuous to determine 6 times, its retention time and peak area are determined, statistical analysis is carried out, relative standard deviation is calculated, to investigate precision.
It the results are shown in Table 3.
The precision (n=6) of 38 kinds of organic acids of table
The retention time of 8 kinds of organic acid reference substances and the RSD values of integrating peak areas are respectively less than 5%, as a result without significant change,
Show that sample precision is good, it is adaptable to quantitative analysis.
The above results show that set up RP-HPLC-DAD analysis methods are quick, accurate, sensitive, it is adaptable in grape juice
The qualitative and quantitative analysis of organic acid.
Embodiment 2
1. the foundation of grape juice false distinguishing model
The Xn that organic acid content present in pure grape juice is designated as X1, X2, X3 respectively ..., then Logistic regression functions
For：
By the π values of pure grape juice
1 is denoted as, the π values of non-pre-packaged pure grape juice are denoted as 0, obtain the parameter fitting of Logistic models：Wherein, x1、x2、
x3、x4、x5、x6、x7Content (the list of tartaric acid, malic acid, ascorbic acid, lactic acid, citric acid, fumaric acid, butanedioic acid is represented respectively
Position：mg/mL).
The checking of 2.Logistic models
With 10 parts of market stochastic buying blind samples (including pure grape juice and grape juice beverage) and self-control incorporation different volumes
Than the grape juice of (basic, normal, high) cider as sample is verified, related data is obtained after carrying out HPLC detections to wherein organic acid,
To calculate its π value, to be verified to Logistic models.
To 13 verification sample random numbers.The pure blind sample of grape juice and grape juice beverage and the adulterated sample numbering of self-control are
59-71.The wherein pure blind sample of grape juice is 59,64,68,70,71, and the pre-packaged blind sample of grape juice beverage is 60,61,63,66,67,
The grape juice sample number into spectrum for mixing different volumes ratio (basic, normal, high) cider is 62,65,69.Sample is drawn by HPLC analyses
Mesotartaric acid, malic acid, ascorbic acid, lactic acid, citric acid, fumaric acid, the content of butanedioic acid, bring above-mentioned equation into, calculate its π
Value, to model preliminary test, the results are shown in Table 4.As a result show, in addition to the result mistake of No. 68 samples, the mirror of remaining 12 sample
Other result is correct, uses this method to differentiate the accuracy of the grape juice true and false for 92.31%.
The content detection data and model inspection table of seven kinds of organic acids in 4 13 samples of table
| Numbering | x1 | x2 | x3 | x4 | x5 | x6 | x7 | π values |
| 59 | 1.01 | 2.57 | 0.05 | 0.76 | 0.41 | 0.03 | 2.66 | 1.00 |
| 60 | 0.98 | 1.87 | 0.38 | 1.17 | 0.22 | 0.28 | 1.17 | 0.00 |
| 61 | 0.61 | 1.97 | 0.24 | 0.12 | 0.51 | 0.71 | 0.05 | 0.00 |
| 62 | 0.97 | 1.69 | 0.28 | 0.29 | 0.20 | 0.29 | 0.04 | 0.00 |
| 63 | 0 | 0.34 | 0.05 | 0 | 1.29 | 0 | 0.27 | 0.00 |
| 64 | 0.99 | 2.41 | 0.09 | 1.05 | 0.66 | 0.18 | 0.73 | 1.00 |
| 65 | 0.82 | 1.76 | 0.29 | 0.18 | 0.29 | 0.41 | 0.12 | 0.00 |
| 66 | 0.64 | 0.65 | 0.54 | 0.02 | 1.20 | 0.04 | 0.12 | 0.00 |
| 67 | 0.49 | 0.69 | 0.65 | 0.08 | 2.60 | 0.05 | 0.02 | 0.00 |
| 68 | 1.36 | 0.35 | 0.14 | 0.01 | 2.50 | 0.01 | 0.10 | 0.00 |
| 69 | 0.59 | 1.85 | 0.30 | 0.23 | 0.32 | 0.24 | 0.06 | 0.00 |
| 70 | 1.61 | 1.47 | 0.14 | 0.12 | 0.61 | 0.21 | 0.05 | 1.00 |
| 71 | 0.77 | 2.52 | 0.05 | 0.77 | 0.36 | 0.02 | 0.15 | 1.00 |
Claims (1)
1. a kind of grape juice authenticity identification method, it is characterised in that comprise the following steps：
1) content of a variety of organic acids in grape juice is determined, with high performance liquid chromatograph while detecting wine after testing sample is extracted
Stone acid, malic acid, ascorbic acid, lactic acid, citric acid, acetic acid, fumaric acid, the content of butanedioic acid, comprise the following steps：
Precision, which is weighed, to be dried to the tartaric acid of constant weight, malic acid, ascorbic acid, lactic acid, citric acid, acetic acid, fumaric acid and amber
Sour each 10mg of reference substance, 10mL is settled to after being dissolved with ultra-pure water, with 0.22 μm of filtering with microporous membrane, is used as reference substance solution；
Testing sample 5mL, plus methanol and pH is taken to be diluted to 25mL, the methanol and phosphorus for 2.8 ammonium dihydrogen phosphate cushioning liquid
The volume ratio of acid dihydride ammonium cushioning liquid is 1: 99, after ultrasound is mixed, and centrifugation takes supernatant 1mL through 0.22 μm of miillpore filter mistake
Filter, is used as sample solution；
Chromatographic condition and system suitability：Chromatographic column is reversed-phase column, and stationary phase is polar embedded C18 bonded silica gels；Methanol and pH
It is mobile phase for 2.8 ammonium dihydrogen phosphate cushioning liquid, the volume ratio of methanol and ammonium dihydrogen phosphate cushioning liquid is 1: 99；DAD
Detector, Detection wavelength is 210nm；30 DEG C of column temperature；Flow velocity is 0.8mL/min；
Accurate reference substance solution of drawing is detected with each 10 μ l of sample solution injection liquid chromatographs respectively；
2) the seven kinds of organic acid contents determined using in true grape juice is modeling indexs, using Logistic homing methods to modeling
Index is handled, and the π values of true grape juice is denoted as into 1, the π values of pseudo- grape juice are denoted as 0, can obtain the ginseng of Logistic models
Number fitting：
<mrow>
<mi>&pi;</mi>
<mo>=</mo>
<mfrac>
<mrow>
<mi>exp</mi>
<mrow>
<mo>(</mo>
<mrow>
<mn>2.612</mn>
<mo>+</mo>
<mn>29.815</mn>
<msub>
<mi>x</mi>
<mn>1</mn>
</msub>
<mo>+</mo>
<mn>3.255</mn>
<msub>
<mi>x</mi>
<mn>2</mn>
</msub>
<mo>-</mo>
<mn>121.056</mn>
<msub>
<mi>x</mi>
<mn>3</mn>
</msub>
<mo>-</mo>
<mn>2.822</mn>
<msub>
<mi>x</mi>
<mn>4</mn>
</msub>
<mo>-</mo>
<mn>14.899</mn>
<msub>
<mi>x</mi>
<mn>5</mn>
</msub>
<mo>-</mo>
<mn>63.457</mn>
<msub>
<mi>x</mi>
<mn>6</mn>
</msub>
<mo>+</mo>
<mn>1.252</mn>
<msub>
<mi>x</mi>
<mn>7</mn>
</msub>
</mrow>
<mo>)</mo>
</mrow>
</mrow>
<mrow>
<mn>1</mn>
<mo>+</mo>
<mi>exp</mi>
<mrow>
<mo>(</mo>
<mrow>
<mn>2.612</mn>
<mo>+</mo>
<mn>29.815</mn>
<msub>
<mi>x</mi>
<mn>1</mn>
</msub>
<mo>+</mo>
<mn>3.255</mn>
<msub>
<mi>x</mi>
<mn>2</mn>
</msub>
<mo>-</mo>
<mn>121.056</mn>
<msub>
<mi>x</mi>
<mn>3</mn>
</msub>
<mo>-</mo>
<mn>2.822</mn>
<msub>
<mi>x</mi>
<mn>4</mn>
</msub>
<mo>-</mo>
<mn>14.899</mn>
<msub>
<mi>x</mi>
<mn>5</mn>
</msub>
<mo>-</mo>
<mn>63.457</mn>
<msub>
<mi>x</mi>
<mn>6</mn>
</msub>
<mo>+</mo>
<mn>1.252</mn>
<msub>
<mi>x</mi>
<mn>7</mn>
</msub>
</mrow>
<mo>)</mo>
</mrow>
</mrow>
</mfrac>
</mrow>
By step 1) content of seven kinds of organic acids that measures substitutes into above formula, calculate π values, and it 1 is true grape juice that π values, which are, and π values are 0
Be pseudo- grape juice, formula, x1、x2、x3、x4、x5、x6、x7Tartaric acid, malic acid, ascorbic acid, lactic acid, lemon are represented respectively
Acid, fumaric acid, the content of butanedioic acid, unit is mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510387717.0A CN105021761B (en) | 2015-07-02 | 2015-07-02 | A kind of method of the content and its true and false of a variety of organic acids in detection grape juice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510387717.0A CN105021761B (en) | 2015-07-02 | 2015-07-02 | A kind of method of the content and its true and false of a variety of organic acids in detection grape juice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105021761A CN105021761A (en) | 2015-11-04 |
| CN105021761B true CN105021761B (en) | 2017-09-12 |
Family
ID=54411886
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510387717.0A Active CN105021761B (en) | 2015-07-02 | 2015-07-02 | A kind of method of the content and its true and false of a variety of organic acids in detection grape juice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105021761B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248847A (en) * | 2016-07-08 | 2016-12-21 | 江苏今世缘酒业股份有限公司 | A kind of method of seven kinds of organic acid contents in wine brewing material of mensuration simultaneously |
| CN107505407A (en) * | 2017-07-19 | 2017-12-22 | 贵州茅台酒股份有限公司 | It is a kind of while determine lactic acid in white wine fermented grain, the method for acetic acid content |
| CN107328937A (en) * | 2017-08-24 | 2017-11-07 | 璞晞（广州）生物免疫技术有限公司 | For the construction method for the practicality model for evaluating hepatitis B hepatopathy NK cell functions |
| CN109765309A (en) * | 2018-12-27 | 2019-05-17 | 辽宁三生科技发展有限公司 | A kind of method of effective component in separation detection citric acid disinfectant |
| CN110672766B (en) * | 2019-10-17 | 2021-08-06 | 中国科学院山西煤炭化学研究所 | C in Fischer-Tropsch synthetic oil1-C4Method for detecting content of organic acid |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3920398A (en) * | 1973-08-06 | 1975-11-18 | Dow Chemical Co | Method for chromatographic analysis of organic acids or their carboxylate salts |
| JP2004125453A (en) * | 2002-09-30 | 2004-04-22 | Shimadzu Corp | Method for analyzing organic acid |
| CN100399022C (en) * | 2004-12-30 | 2008-07-02 | 中国科学院东北地理与农业生态研究所 | Method for detecting low-molecular-weight organic acid in secretion of root system of plant |
| CN101101284A (en) * | 2007-07-26 | 2008-01-09 | 复旦大学 | Method for simultaneously determining multiple organic acid content of fruit |
| CN102818872A (en) * | 2012-08-08 | 2012-12-12 | 福建省农业科学院农业工程技术研究所 | High performance liquid chromatography for simultaneously detecting content of ten organic acids in fruit |
| JP2014202570A (en) * | 2013-04-04 | 2014-10-27 | 大日本住友製薬株式会社 | Separation method of organic acid ions and/or inorganic acid ions |
-
2015
- 2015-07-02 CN CN201510387717.0A patent/CN105021761B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105021761A (en) | 2015-11-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105021761B (en) | A kind of method of the content and its true and false of a variety of organic acids in detection grape juice | |
| CN105628818B (en) | The method that high performance liquid chromatograph detects six kinds of sweeteners in feed addictive simultaneously | |
| CN102103128A (en) | Method for determining contents of formaldehyde, acetaldehyde and acetone in water-borne adhesives for cigarettes | |
| CN104122363B (en) | A kind of assay method of methylcobalamin tablet related substance | |
| CN106053627A (en) | Method for simultaneously detecting 26 organic acids and inorganic negative ions in juice | |
| CN105866302A (en) | Method for utilizing ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit | |
| CN104155377B (en) | A kind of method adopting ammonia content in ion chromatography tobacco juice for electronic smoke | |
| CN102955011A (en) | Gas chromatography detection method of ethylene glycol, diethylene glycol and menthol in toothpaste | |
| CN103197022A (en) | Method for detecting amino acid contained in table vinegar | |
| CN104535668B (en) | The HPLC analytical method of polyphenol substance in a kind of grape wine | |
| CN102305833A (en) | Measurement method of content of nitrites in polypropylene tow for cigarettes | |
| CN113777190A (en) | Method for separating and measuring sanshool content in pepper extract and pepper extract product and application of sanshool content | |
| CN103235051B (en) | Method for determining colorant solvent green 7 in dry food packaging paper | |
| CN105301157A (en) | Quality control method of related substances of methanesulfonic acid kukoamine B | |
| CN109100456B (en) | Method for simultaneously determining content of 3 fat-soluble vitamins in multivitamin injection | |
| CN105572237A (en) | High performance liquid chromatographic method for rapid quantitative assessment of honey quality | |
| CN101658550A (en) | Method for measuring content of selfheal oral liquid | |
| CN111007169A (en) | Construction method and quality evaluation method of fingerprint of strong loquat syrup | |
| CN103424499B (en) | Method for detecting picrasma quassioides alkali content in picrasma quassioides | |
| CN102507757A (en) | Method for measuring ascorbic acid content in porphyra yezoensis by high performance liquid chromatography | |
| CN101581707A (en) | Method for simultaneously detecting acetylmethylcar-binol and ligustrazine in table vinegar | |
| CN108693274B (en) | Method for detecting triazole pesticide residues in white wine by combining solidification-floating dispersion liquid microextraction and HPLC | |
| CN107976494B (en) | Construction of standard characteristic spectrum of Kangfu tincture and quality detection method thereof | |
| CN106872629A (en) | A kind of method of three nitrogen amidine contents in measure dairy products | |
| CN106324169A (en) | Solid phase extraction-gas chromatography-tandem mass spectrum detection method for amide fungicides in wine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |