CN105018367B - The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant - Google Patents
The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant Download PDFInfo
- Publication number
- CN105018367B CN105018367B CN201410168402.2A CN201410168402A CN105018367B CN 105018367 B CN105018367 B CN 105018367B CN 201410168402 A CN201410168402 A CN 201410168402A CN 105018367 B CN105018367 B CN 105018367B
- Authority
- CN
- China
- Prior art keywords
- resistant
- bacillus
- plant
- habitat
- ocean
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention discloses one plant of degeneration-resistant ocean habitat bacillus of induction plant disease-resistant, to obtain bacillus marinus CT2628 bacterial strain (Bacillus sp.) from ocean habitat, through induced mutations breeding and training systern, the condition of its suitable artificial cultivation and fermentation are as follows: with its deposit number are as follows: the CT2628 bacterial strain of CGMCC No.8921, the formula of its suitable fermentation culture are as follows: beef extract 1-3g, peptone 2-3g, yeast powder 1-3g, glucose 1-3g, NaCl, 20-25g, KCl0.3-0.5g, MgCl2·7H2O1.9-2.5g, FePO40.01-0.02g, water 1000ml, pH7.0-8.0 above-mentioned are culture solution.Advantage: have induction plant disease-resistant, salt resistance, cold-resistant ocean habitat bacillus and agriculturally applying.
Description
Technical field
The invention belongs to marine microorganism application fields, the bacillus CT2628 specifically obtained by ocean habitat
Bacterial strain (Bacillus sp.) has diseases prevention, induction to cultivated plant by induced mutations breeding and cultivation and fermentation, active bacteria formulation
The effect of cold-resistant salt resistance.The culture presevation in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC),
Address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, preservation date March 17 in 2014
Day, the CT2628 bacterial strain of deposit number CGMCC No.8921.
Background technique
Currently, environmental protection and food-safety problem obtain the common concern of government, both the central and local governments, reduce chemical agriculture
No matter the use of medicine all has important practical significance, biology to protection environment, or the safety of the source agricultural product to food
Pesticide is low with its toxicity, and the good feature of being compatible with the environment property is taken seriously in world wide, and active bacteria formulation is the one of biological pesticide
The effects of big type, these beneficial bacteriums are by colonizing in plant or colonizing in pest body, play antibacterial and desinsection.Gemma bar
Bacterium is that wherein foremost probiotics, the producing strains as various enzymes are widely used in industrial production；As feed addictive and
Medicine can inhibit common enteric pathogenic bacteria, improve animal disease resistant ability, reduce antibiotic dosage；Its nontoxic, at low cost, desinsection,
The strong feature of bacteriostasis, it is also of interest to research and develop biological pesticide both at home and abroad, it is the maximum biological pesticide of world wide production, it is new to provide
The discovery in source, new application is even more the object of domestic and international pesticide field contention.
China is one of serious country of the soil salinization, and the salinization soil gross area is equivalent to cultivated up to 34,600,000 hectares
The 1/3 of ground, the characteristics of in view of having a large population and a few land, these salinization soils still utilize plantation salt tolerant crop by people, meanwhile, early spring
Crops massive losses are also resulted in every year with the cold spell in later spring in late fall.Microbial pesticide is developed both at home and abroad at present since biocontrol microorganisms all come
Derived from land, it is difficult to survive in the higher soil of salt content, colonizes, it is poor in the control efficiency of salt lick soil-borne disease；Land
Due to habitat, lower environment temperature limits it and plays a role ground biocontrol microorganisms.That was paid close attention to both at home and abroad in recent years is new
One marine microorganism of resource makes it be easier to send out in salt-affected soil and low temperature environment due to the low temperature in its original habitat characteristic with high salt
The effect of waving shows its habitat advantage in novel pesticide development field.
Summary of the invention
The object of the present invention is to provide one plant induce plant disease-resistant, salt resistance, cold-resistant bacillus marinus and may be in agriculture
The application of industry production, invention take full advantage of the natural resources of this special habitats of marine microorganism, and wild strain has passed through people
Work mutation breeding, biological characteristics and pesticide activity are stablized, and are easy culture, cultivation cycle is short, and operating method is simple and feasible, to ring
Border is not polluted, and raw materials used cheap, production cost is low, and development prospect is good, industrialization easy to accomplish.
The purpose of the present invention is implemented by the following technical solutions, to obtain bacillus marinus CT2628 from ocean habitat
Bacterial strain (Bacillus sp.), through induced mutations breeding and training systern, the condition of suitable artificial cultivation and fermentation are as follows:
1) with its deposit number are as follows: the CT2628 bacterial strain of CGMCC No.8921, bacterial strain are tested through medium optimization, are fitted
The formula of suitable fermentation culture are as follows: beef extract 1-3g, peptone 2-3g, yeast powder 1-3g, glucose 1-3g, NaCl, 20-
25g, KCl0.3-0.5g, MgCl2·7H2O1.9-2.5g, FePO40.01-0.02g, water 1000ml, pH7.0-8.0, it is above-mentioned to be
Culture solution, adding agar 16-19g is solid medium；
2) bacterial strain sets 23 DEG C of -28 DEG C of incubator cultures 3-5 days in above-mentioned solid medium, by the good bacterium of solid culture basal growth
Body scrapes, and culture solution culture 3 days accessed in step 1 are seed liquor, then seed liquor is accessed the above-mentioned culture of 40-50L by 1:10
In liquid, 23 DEG C -28 DEG C, revolving speed 150rpm, 3-5 days acquisition fermentation materials of shaken cultivation, same methods as needed are expanded to
Target culture amount.
The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant, it is characterised in that: strain characteristic is bacterium
Turbid yellow is fallen, there is pleat on surface mostly, and sometimes smooth, the later period generates dark blue pigment on peptone culture medium；Thallus is rod-shaped, and leather is blue
2.9-5.9 μm of μ m of Albert'stain Albert positive 0.6-1.9, no pod membrane, peritrichous is raw in gemma, is gathered into chain, hydrolyzes starch,
Gelatin, catalase, VP test are the positive, can produce acid using glucose, xylose, arabinose, and tyrosine hydrolysis is negative, regardless of
Lignin is solved, nitrate is restored, utilizes citrate.
The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant, feature exist: by ocean habitat bud
The processing of spore bacillus CT2628 bacterial strain, tomato Electrolyte Leakage Rate and mda content reduce respectively under the conditions of 0 DEG C -2 DEG C of low temperature
98.66% and 63.12%, soluble sugar, superoxide dismutase, peroxidase, proline be respectively increased
62.47%, 62.16%, 205.13%, 342.11%, under salt damage NaCl0.5-0.6% stress, malonaldehyde is reduced
72.38%, soluble sugar, superoxide dismutase, peroxidase, proline have been respectively increased 80.47%, 89.33%,
117.44%, 230.53%, CT2628 bacterial strain inducing plant cold resistance and salt resistance significant effect.
The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant, it is characterised in that: CT2628 bacterial strain is adopted
It is obtained with conventional opposite culture method and bacteriostasis rate statistic law screening, target bacterium is to endanger serious 9 kinds of plants in agricultural production
Disease fungus: Rice Blast Fungus Pyricularia oryzae, eggplant pink mold Alternaria solani, Fusarium
Fusarium oxysporum, the pathogen of Botrytis cinerea Botrytis cinerea, verticillium dahliae Verticillium dahliae,
Bud branch mould Cladosporium fulvum, phytophthora blight of pepper Phytophthora capsici, rhizoctonia Rhizoctonia
Solani gibberella Fusarium graminearum carries out antibacterial test, opposite culture method inhibition zone 26- as target bacterium
35mm, bacteriostasis rate statistic law bacteriostasis rate 78.00-88.20%, belongs to strong bacteriostatic activity.
The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant, it is characterised in that: bacillus marinus
Bacterial strain can normally be determined in the cucumber of the plantation of common plantation soil and beach salinized soil, tomato, capsicum, wild cabbage, muskmelon, eggplant
It grows, the highest field planting number after leaching root plantation 4-6 days in plant roots, stem, leaf is respectively up to 6.00 × 103、8.30×103、4.65×
103, colonizing group's number can maintain 15-20 days.
The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant, it is characterised in that: ocean habitat gemma
Bacillus strain is to 4 kinds of cucumber, tomato, the white powder of capsicum, early epidemic, phytophthora, grey mold diseases, and preventive effect is up to 77.1%-
89.0%, therapeutic effect 59.0%-62.4%, it is anti-to be above traditional chemical pesticide carbendazim with the effect controlled.
Beneficial effects of the present invention: there is induction plant disease-resistant, salt resistance, the ocean habitat bacillus of cold-resistant and in agricultural
The application of production, takes full advantage of the natural resources of this special habitats of marine microorganism, and wild strain has passed through induced mutations
Breeding, biological characteristics and pesticide activity are stablized, and are easy culture, cultivation cycle is short, and operating method is simple and feasible, does not have to environment
Pollution, raw materials used cheap, production cost is low, and development prospect is good, industrialization easy to accomplish.
Specific embodiment
Protection scope of the present invention is not only limited in following embodiment, and steps are as follows for particular technique of the invention:
The artificial culture and fermentation of embodiment 1:CT-2628 bacterial strain
With deposit number are as follows: the CT-2628 bacterial strain of CGMCC No.8921 is tested, beef extract 1-3g through medium optimization,
Peptone 2-3g, yeast powder 1-3g, glucose 1-3g, NaCl, 20-30g, KCl0.3-0.5g, MgCl2·7H2O1.9-2.5g,
FePO40.01-0.02g, water 1000ml, pH7.0-8.0 above-mentioned are culture solution, and adding agar 16-19g is solid medium；
Cultivate on above-mentioned solid medium, and test its physicochemical characteristics with conventional method, strain characteristic is as follows: bacterial strain is special
Property be the turbid yellow of bacterium colony, there is pleat on surface mostly, and sometimes smooth, the later period generates dark blue pigment on peptone culture medium；Thallus bar
Shape, 2.9-5.9 μm of μ m of Gram-positive 0.6-1.9, no pod membrane, peritrichous is raw in gemma, is gathered into chain, water
Starch is solved, gelatin, catalase, VP test are the positive, and acid, tyrosine hydrolysis yin can be produced using glucose, xylose, arabinose
Property, lignin is not decomposed, restores nitrate, utilizes citrate.
Bacterial strain sets 23 DEG C of -28 DEG C of incubator cultures 3-5 days in above-mentioned solid medium, by the good thallus of solid culture basal growth
It scrapes, culture solution culture 3 days accessed in step 1 are seed liquor, then seed liquor is accessed the above-mentioned culture solution of 40-50L by 1:10
In, 23 DEG C -28 DEG C, revolving speed 150rpm, 3-5 days acquisition fermentation materials of shaken cultivation, same methods as needed are expanded to mesh
Mark culture amount；
Antifungal Activity in Vitro of the embodiment 2:CT-2628 bacterial strain to various plants disease fungus:
For fermentation liquid using conventional opposite culture method, bacteriostasis rate statistic law, target bacterium is to endanger serious 9 in agricultural production
Kind plant pathogenic fungi: Rice Blast Fungus Pyricularia oryzae, eggplant pink mold Alternaria solani, Fusarium
Fusarium oxysporum, the pathogen of Botrytis cinerea Botrytis cinerea, verticillium dahliae Verticillium dahliae,
Bud branch mould Cladosporium fulvum, phytophthora blight of pepper Phytophthora capsici, rhizoctonia Rhizoctonia
The antibacterial test result of solani gibberella Fusarium graminearum progress shows as follows:
Table 1CT-2628 is to phytopathogen bacteriostatic activity
Annotation: phytopathogen: Po:Pyricularia oryzae；As:A " ternaria salani；
Fo:Fusarium oxysporum:Bc:Botrytis cinerea；
Vd:Verticillium dahliae；Cf:Cladosporium fulvum；
Pc:Phytophthora capsici；Rs:Rhizoctonia solani；
Fg:Fusarium graminearum
Test 1, abbreviation T1: opposite culture test indicates strain activity with inhibition zone size, and "+" indicates that inhibition zone is 20-
25mm " ++ " expression inhibition zone is that 26-30mm " +++ " expression inhibition zone is 30-35mm；
Test 2, abbreviation T2: to the inhibiting rate of pathogen, inhibiting rate=(control colony radius-processing colony radius)/right
According to colony radius × 100%
By upper table data it is found that CT-2628 bacterial strain endangers serious disease fungus to 9 kinds of agriculturals, have antibacterial well
Effect, in opposite culture method, except rhizoctonia Rhizoctonia solani gibberella Fusarium graminearum is antibacterial
Circle is 26-30mm, and other 7 kinds all reach 30-35mm, in bacteriostasis rate statistic law, bacteriostasis rate 78.00-88.20%.That is inhibition zone
26-35mm, bacteriostasis rate 78.00-88.20% belong to strong bacteriostatic activity, have the potentiality for developing novel germicide.
Embodiment 3: CT-2628 bacterial strain plant living body field planting
Institute's method for testing is plant fixing traditional test methods: above method culture is sent out after CT-2628 does rifampin label
Ferment, tomato seedling, cucumber seedling immerse zymocyte liquid, are planted respectively into common plantation soil and beach salinized soil (NaCl content 0.5%)
Basin alms bowl is sampled to cultivate on the plating medium for adding sharp good fortune and be seen respectively at 1,3,5,7,10,15 day by each tissue grinder of seedling
Examine, and be converted to every gram of sample bacterial clump number (cfu/g), as a result prove CT-2628 bacterial strain no matter common plantation soil also
It is that the cucumber of the plantation of beach salinized soil, tomato, capsicum, wild cabbage, muskmelon, eggplant can be normally colonized, after leaching root is planted 5 days
Root, stem, leaf highest field planting number respectively up to 6.00 × 103、8.30×103、4.65×103, after processing after 15 days, CT-2628
It remains to maintain a preferable level inside tomato.
Table 2 soaks CT-2628 colonisation in plant after root is planted 5 days
The effect of embodiment 4:CT-2628 strains on plant living body controlling disease
Conventional method does prevention and treatment to plant disease and treatment is tested, and prevention and treatment first sprays bacterial strain CT-2628 on potted plant
Fermentation liquid after 24 hours, sprays pathogen spore suspension；Treatment is first inoculated with pathogen on the contrary, after spray bacterial strain fermentation liquor, 1 week
After carry out Disease investigation, to spray triadimefon as positive control, spray clear water and be used as negative control, calculating control efficiency (%).
Upper table number it was demonstrated that potted plant disease prevention and treatment, CT-2628 has anti-well 4 kinds of cultivated plant diseases
Control effect, wherein preventive effect is best, prevention and treatment efficiency up to 77.1%-89.0%, the slightly lower 59.0%-62.4% of therapeutic effect,
Prevention and treatment effect is above traditional chemical pesticide carbendazim.
Embodiment 5:CT-2628 strains on plant induces degeneration-resistant effect
Institute's method for testing is that plant induces degeneration-resistant traditional test methods: (1) cold-resistant is tested: tomato seedling is planted to be ploughed into common
The basin alms bowl for making soil sets low (0 DEG C -2 DEG C) of temperature respectively and cultivates 2 days, spray CT-2628 fermentation liquor treatment seedling and clear water (compareing CK) into
Row compares；(2) salt resistance is tested: tomato seedling plants the basin alms bowl into beach salinized soil (NaCl content 0.5%-0.6%), cultivates 10 days,
Pouring CT-2628 fermentation liquor treatment seedling is compared with clear water (compareing CK) and tests respectively Electrolyte Leakage Rate (REC), solvable
Property sugared (SS), superoxide dismutase (SOD), peroxidase (POD), malonaldehyde (MDA), the content of proline (Pro)
Table 3CT-2628 induces the variation of the indexs such as plant stress-resistance process tomato defensive ferment
Note: 1. Electrolyte Leakage Rate (REC), soluble sugar (SS), superoxide dismutase (SOD), peroxidase
(POD), malonaldehyde (MDA), proline (Pro), 2. "-" expression are not surveyed, and negative number representation reduces
Plant aggrieved, content of lipid peroxidation product (MDA), to membrane damage journey under the conditions of the environment stresses such as salt, cold
Degree has important indicative function.Electrolyte Leakage Rate (REC) be cytoplasma membrane whether the index by cold damage, conductivity gets over
Height illustrates that the injury being subject to is heavier.The soluble sugar content of plant cell and cold-resistant, the salt-resistance of plant are positively correlated, solvable
Property sugar protection plant protoplast and protein.Proline (Pro), superoxide dismutase (SOD), peroxidase (POD)
Accumulation, by maintaining osmotic adjustment, stable protein protects the stabilization of membrane structure, and therefore, these reactions are by as weighing apparatus
Measure the important indicator of plant resistance.
Plant is under adverse environmental factor, and Electrolyte Leakage Rate (REC) and malonaldehyde (MDA) are lower, soluble sugar (SS), super oxygen
Compound mutase (SOD), peroxidase (POD), proline (Pro) is higher, and anti-adversity ability is stronger, and table 3 is shown in 0 DEG C -2 DEG C
Cryogenic conditions under CT2628 bacterial strain inducing plant cold resistance significant effect, tomato Electrolyte Leakage Rate and mda content difference
98.66% and 63.12% are reduced, soluble sugar, superoxide dismutase, peroxidase, proline are respectively increased
62.47%, 62.16%, 205.13%, 342.11%.Under salt damage NaCl0.5-0.6% stress, handled through CT2628 bacterial strain,
Malonaldehyde reduces 72.38%, and soluble sugar, superoxide dismutase, peroxidase, proline are respectively increased, solvable
Property sugar, superoxide dismutase, peroxidase, proline have been respectively increased 80.47%, 89.33%, 117.44%,
230.53%, the variation indirect proof CT2628 bacterial strain inducing plant stress-resistance significant effect of these indexs.
Although specific example of the invention is described above, it is to those skilled in the art a bit
It will be evident that can the present invention be made various changes and be changed under the premise without departing from the spirit and scope of the present invention, all at this
It in the protection scope of invention, modifies for above-described embodiment, adds and replace, all without departing from protection model of the invention
It encloses.
Claims (4)
1. the degeneration-resistant ocean habitat bacillus (Bacillus sp.) of one plant of induction plant disease-resistant, to obtain sea from ocean habitat
Foreign bacillus CT2628 bacterial strain, deposit number are as follows: CGMCC No.8921.
It is described disease-resistant to be directed to 2. ocean habitat according to claim 1 bacillus is in the application of induction plant disease-resistant
Serious plant pathogenic fungi: Rice Blast Fungus Pyricularia oryzae, eggplant pink mold is endangered in agricultural production
It is Alternaria solani, Fusarium Fusarium oxysporum, the pathogen of Botrytis cinerea Botrytis cinerea, big beautiful
Verticillium dahliae Verticillium dahliae, bud branch mould Cladosporium fulvum, phytophthora blight of pepper Phytophthora
Capsici, rhizoctonia Rhizoctonia solani or gibberella Fusarium graminearum；The plant is Huang
Melon, tomato or capsicum.
3. ocean habitat according to claim 1 bacillus is in the application of induction plant stress-resistance, which is characterized in that described
It is degeneration-resistant be salt resistance or cold-resistant.
4. application according to claim 2 or 3, ocean habitat bacillus by added in fermentation culture agar come
Culture, the fermentation culture includes beef extract 1-3g, peptone 2-3g, yeast powder 1-3g, glucose 1-3g, NaCl20-
25g, KCl 0.3-0.5g, MgCl2·7H2O 1.9-2.5g, FePO40.01-0.02g, water 1000ml, pH 7.0-8.0；It is described
The additive amount of agar is 16-19g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168402.2A CN105018367B (en) | 2014-04-18 | 2014-04-18 | The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168402.2A CN105018367B (en) | 2014-04-18 | 2014-04-18 | The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105018367A CN105018367A (en) | 2015-11-04 |
| CN105018367B true CN105018367B (en) | 2019-06-18 |
Family
ID=54408672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410168402.2A Expired - Fee Related CN105018367B (en) | 2014-04-18 | 2014-04-18 | The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105018367B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106591193B (en) * | 2016-12-23 | 2019-07-26 | 河北省农林科学院遗传生理研究所 | One plant of bacillus amyloliquefaciens with the degeneration-resistant effect of wide spectrum growth-promoting |
| CN109097302A (en) * | 2018-08-16 | 2018-12-28 | 浙江农林大学 | A kind of bacillus is promoting the application in plant growth |
| CN108998394B (en) * | 2018-08-16 | 2020-11-20 | 浙江农林大学 | Bacillus and application thereof |
| CN109825456B (en) * | 2019-03-15 | 2023-04-18 | 青海省农林科学院 | Marina marine bacillus E40208a1 and application thereof |
| CN110018201B (en) * | 2019-04-03 | 2020-06-12 | 上海交通大学 | Rapid identification method for resistance of corn variety fusarium ear rot |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101331881A (en) * | 2007-06-27 | 2008-12-31 | 国家海洋局第一海洋研究所 | Sea bacillus wettable powder, and preparation method and use thereof |
| CN102108339A (en) * | 2010-11-26 | 2011-06-29 | 沈阳农业大学 | Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof |
| KR20140020022A (en) * | 2012-08-07 | 2014-02-18 | 한국해양과학기술원 | New compounds having biological activities comprising antimicrobial activity, marine microorganism producing the new compounds |
| KR101371554B1 (en) * | 2011-12-21 | 2014-03-10 | 한국해양과학기술원 | New fatty acids having physiological activity comprising antimicrobial activity, Marine microorganism producing the new fatty acids and producing method of the new fatty acids using the marine microorganism |
-
2014
- 2014-04-18 CN CN201410168402.2A patent/CN105018367B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101331881A (en) * | 2007-06-27 | 2008-12-31 | 国家海洋局第一海洋研究所 | Sea bacillus wettable powder, and preparation method and use thereof |
| CN102108339A (en) * | 2010-11-26 | 2011-06-29 | 沈阳农业大学 | Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof |
| KR101371554B1 (en) * | 2011-12-21 | 2014-03-10 | 한국해양과학기술원 | New fatty acids having physiological activity comprising antimicrobial activity, Marine microorganism producing the new fatty acids and producing method of the new fatty acids using the marine microorganism |
| KR20140020022A (en) * | 2012-08-07 | 2014-02-18 | 한국해양과학기술원 | New compounds having biological activities comprising antimicrobial activity, marine microorganism producing the new compounds |
Non-Patent Citations (2)
| Title |
|---|
| 几株海洋生境芽孢杆菌和木霉菌农药潜力的研究;陶晶晶;《万方.中国学位论文全文数据库》;20120428;摘要、第27页第1-2段、第30页表3-2、第32页第1段 * |
| 海洋生境芽孢杆菌(Bacillus spp.)的培养条件及产生的胞外抗菌蛋白;田黎等;《海洋学报(中文版)》;20010713;第23卷(第04期);87-92 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105018367A (en) | 2015-11-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105886428B (en) | One plant of Streptomycesalbidoflhaving and its application in microbial manure | |
| CN105420156B (en) | One plant of bacillus amyloliquefaciens with phosphorus decomposing disease prevention growth-promoting effect and its application | |
| CN104232509B (en) | A kind of Bacillus amyloliquefaciens strain Bg-1 and application thereof | |
| KR101279044B1 (en) | Pantoea dispersa WCU35 strain, composition for control plant disease and control method of plant disease with same | |
| CN104498386B (en) | The preparation method and application of raw Bacillus amyloliquefaciens strain SZ23 and zymotic fluid in wild jujube | |
| CN103045515B (en) | Bacteria agent of a kind of Methylotrophic genus bacillus and its preparation method and application | |
| CN105018366B (en) | Methylotrophic bacillus and its application | |
| CN111471624A (en) | Bacillus belgii CSQXDZ26 strain and application thereof | |
| CN107151641B (en) | Brevibacillus laterosporus for inhibiting rhizoctonia solani and application thereof | |
| CN105018367B (en) | The degeneration-resistant ocean habitat bacillus of one plant of induction plant disease-resistant | |
| CN103952348B (en) | Brevibacterium frigoritolerans as well as microbial inoculant and application of brevibacterium frigoritolerans and microbial inoculant | |
| CN103103149A (en) | Bacillus subtilis S001, application of bacillus subtilis S001, microbial preparation and preparation method of microbial preparation | |
| CN106244504B (en) | Bacillus subtilis and its microbial inoculum with degrading organic phosphor and bacteriostasis | |
| CN106135285A (en) | The application in prevention and control rice blast of the Rhodopseudomonas palustris biocontrol agent | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| CN111690578B (en) | Salt and alkali resistant Siamese bacillus and production method and application of viable bacteria preparation thereof | |
| CN104480046A (en) | Brevibacillus laterosporus strain and application thereof | |
| CN108998394A (en) | A kind of bacillus and its application | |
| CN109929784A (en) | One bacillus subtilis and its cultural method and application | |
| CN103087960A (en) | Bacillus amyloliquefaciens FQS38 and application thereof | |
| CN106399152A (en) | Streptomyces having inhibitory effect on phytophthorasojae root rot fungi, biocontrol preparation and preparation method of biocontrol preparation | |
| CN107287130A (en) | A kind of Streptomycesalbidoflhaving bacterial strain and its application in agricultural chemicals | |
| CN105794853A (en) | Application of rhodopseudomonas palustris fermentation liquor to rice blast prevention and control | |
| CN106754567B (en) | A kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS of efficiently prevention various crop droop | |
| CN103382449B (en) | Streptomyces sp. T2-10 with broad-spectrum insecticidal and antibacterial activity and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190618 Termination date: 20200418 |