CN105017382A - Method for preparing soluble silk protein solution through waste silk - Google Patents
Method for preparing soluble silk protein solution through waste silk Download PDFInfo
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- CN105017382A CN105017382A CN201510499103.1A CN201510499103A CN105017382A CN 105017382 A CN105017382 A CN 105017382A CN 201510499103 A CN201510499103 A CN 201510499103A CN 105017382 A CN105017382 A CN 105017382A
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Abstract
The invention discloses a method for preparing a soluble silk protein solution through waste silk. The method includes the steps that the waste silk is placed in distilled water, soaked for 30-50 min and boiled, boiling is kept for 5-10 min, the wet silk is filtered and collected, the wet silk is added into a boiling calcium chloride solution with the mass fraction being 39-48%, boiling is kept for 10-15 min, filtering is conducted after cooling, filtered liquid is collected, and a rough silk protein solution is obtained; then, dialysis is carried out for 16-18 hours in clean water and carried out in distilled water for 1.5-2 hours, and the soluble silk protein solution is prepared. The dried waste silk without sericin is adopted to serve as the raw material, resources are fully used, and cost is lowered. High-concentration calcium chloride safe to use and low in price is selected to serve as the solution dissolving the silk protein, calcium ions can be filtered out through an infiltration method in the reaction process, and remaining calcium ions and amino acid in the silk protein can form a chelate, so that swelling, fiber separation and breaking happen to the silk protein, the molecular structure is loose, and the silk protein has good water solubility.
Description
Technical field
The present invention relates to daily-use chemical industry technical field, be specifically related to a kind of side utilizing waste silk to produce solubility fibroin liquid.
Background technology
Natural silk, primarily of outer silk gum and internal layer fibroin composition, contains fibroin about 75% in silk.The structure of natural silk and human skin fairly similar, therefore have again the laudatory title of human body " Second Skin ".Silk beauty treatment have long history in China, as far back as the Ming Dynasty of China, silk because of its important pharmaceutical use, by imperial palace highest-ranking imperial concubines for daily beauty and health care.Record according to Compendium of Material Medica: silk fibroin powder can eliminate skin blackspot, therapeutic pyodermia.Modern medical experiment proves further, and the protein content of silk is much higher than pearl, and wherein nitrogen content is higher than pearl 37 times, and primary amino acid content is up to more than 10 times.These amino acid can be directly human hair, and skin absorbs and absorption.Namely more easily permeate at the skin of human epidermal, accelerate the metabolism of skin.Silk contracting amino acid can also suppress melanic generation in skin effectively.Nowadays, beauty treatment circle has started one silk beauty treatment agitation especially, take silk as the fibroin makeup that raw material develops, and the skin care becoming the countless women that likes to be beautiful is first-selected.But silk-protein is due to hydrogen bond action widely, makes between Silk Protein Molecules and combine closely in molecule and be insoluble in water.
Summary of the invention
The waste silk that utilizes that the object of this invention is to provide a kind of economic environmental protection produces the method for solubility fibroin liquid.
In order to solve the problem existing for background technology, the present invention is by the following technical solutions: a kind of method utilizing waste silk to produce solubility fibroin liquid, described method is boiled after waste silk is put into distilled water immersion 30 ~ 50min, keep boiling 5 ~ 10min, the silk that collecting by filtration infiltrates, the massfraction silk of described infiltration being added boiling is in the calcium chloride solution of 39 ~ 48%, constantly boiling 10 ~ 15min, cooled and filtered, collect filtrate, obtain the thick solution of silk-protein, described silk protein solution is loaded in the dialysis tubing of relative molecular weight 8000 ~ 14000, dialyse 16 ~ 18h in clear water, dialyse 1.5 ~ 2h again in distilled water, obtained solubility fibroin liquid.
Further, described waste silk will remove silk gum before using and air-dry.
Further, described calcium chloride solution also can be lithium chloride, strontium chloride, lithiumbromide, strontium bromide or Calcium Bromide.
Further, described solubility fibroin liquid is solution that is tasteless, white clear.
The present invention has following beneficial effect: raw material of the present invention adopts removes silk gum and air-dry waste silk, makes full use of resource, reduces costs; The solution dissolving silk-protein selects the high calcium chloride concentration of use safety, price material benefit, in reaction process, calcium ion filters out by osmose process, remaining calcium ion can also form inner complex with amino acid in silk-protein, make silk-protein that swelling, point fine, fracture occur, molecular structure relaxes, and has good water-soluble.
Embodiment
The present invention will be further described in detail below.
Embodiment one: boil after waste silk being put into distilled water immersion 30min, keep boiling 10min, the silk that collecting by filtration infiltrates, the massfraction silk of described infiltration being added boiling is in the calcium chloride solution of 45%, constantly boiling 10min, cooled and filtered, collects filtrate, obtains the thick solution of silk-protein, described silk protein solution is loaded in relative molecular weight 8000 ~ 14000 dialysis tubing, dialyse 16h in clear water, then the 1.5h that dialyses in distilled water, obtained solubility fibroin liquid.
Embodiment two: boil after waste silk being put into distilled water immersion 40min, keep boiling 7min, the silk that collecting by filtration infiltrates, the massfraction silk of described infiltration being added boiling is in the calcium chloride solution of 39%, constantly boiling 15min, cooled and filtered, collects filtrate, obtains the thick solution of silk-protein, described silk protein solution is loaded in the dialysis tubing of relative molecular weight 11000, dialyse 17h in clear water, then the 2h that dialyses in distilled water, obtained solubility fibroin liquid.
Embodiment three: boil after waste silk being put into distilled water immersion 50min, keep boiling 5min, the silk that collecting by filtration infiltrates, the massfraction silk of described infiltration being added boiling is in the calcium chloride solution of 48%, constantly boiling 10min, cooled and filtered, collects filtrate, obtains the thick solution of silk-protein, described silk protein solution is loaded in the dialysis tubing of relative molecular weight 14000, dialyse 18h in clear water, then the 2h that dialyses in distilled water, obtained solubility fibroin liquid.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, other amendments that those of ordinary skill in the art make technical scheme of the present invention or equivalently to replace, only otherwise depart from the spirit and scope of technical solution of the present invention, all should be encompassed in the middle of right of the present invention.
Claims (4)
1. the method utilizing waste silk to produce solubility fibroin liquid, it is characterized in that, described method is boiled after waste silk is put into distilled water immersion 30 ~ 50min, keep boiling 5 ~ 10min, the silk that collecting by filtration infiltrates, the massfraction silk of described infiltration being added boiling is in the calcium chloride solution of 39 ~ 48%, constantly boiling 10 ~ 15min, cooled and filtered, collect filtrate, obtain the thick solution of silk-protein, described silk protein solution is loaded in the dialysis tubing of relative molecular weight 8000 ~ 14000, dialyse 16 ~ 18h in clear water, dialyse 1.5 ~ 2h again in distilled water, obtained solubility fibroin liquid.
2. a kind of method utilizing waste silk to produce solubility fibroin liquid according to claim 1, is characterized in that, described waste silk will remove silk gum before using and air-dry.
3. a kind of method utilizing waste silk to produce solubility fibroin liquid according to claim 1, is characterized in that, described calcium chloride solution also can be lithium chloride, strontium chloride, lithiumbromide, strontium bromide or Calcium Bromide.
4. a kind of method utilizing waste silk to produce solubility fibroin liquid according to claim 1, is characterized in that, described solubility fibroin liquid is solution that is tasteless, white clear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510499103.1A CN105017382A (en) | 2015-08-14 | 2015-08-14 | Method for preparing soluble silk protein solution through waste silk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510499103.1A CN105017382A (en) | 2015-08-14 | 2015-08-14 | Method for preparing soluble silk protein solution through waste silk |
Publications (1)
| Publication Number | Publication Date |
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| CN105017382A true CN105017382A (en) | 2015-11-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510499103.1A Pending CN105017382A (en) | 2015-08-14 | 2015-08-14 | Method for preparing soluble silk protein solution through waste silk |
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| CN (1) | CN105017382A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106750773A (en) * | 2016-12-06 | 2017-05-31 | 宁波芸生纺织品科技有限公司 | A kind of biological packing material and preparation method thereof |
| CN107216376A (en) * | 2017-07-28 | 2017-09-29 | 浙江理工大学 | A kind of extracting method of tussah silk fibroin |
| CN108867165A (en) * | 2018-07-23 | 2018-11-23 | 王晚秀 | A kind of preparation method of silk gum paper sizing agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101381758A (en) * | 2008-10-23 | 2009-03-11 | 天津大学 | Method for extracting fibroin peptide from mulberry silk |
| CN102532253A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Method for extracting silk protein |
| CN104761616A (en) * | 2015-04-15 | 2015-07-08 | 海安县南莫制丝有限公司 | Method for preparing soluble silk protein liquid employing waste silk |
-
2015
- 2015-08-14 CN CN201510499103.1A patent/CN105017382A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101381758A (en) * | 2008-10-23 | 2009-03-11 | 天津大学 | Method for extracting fibroin peptide from mulberry silk |
| CN102532253A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Method for extracting silk protein |
| CN104761616A (en) * | 2015-04-15 | 2015-07-08 | 海安县南莫制丝有限公司 | Method for preparing soluble silk protein liquid employing waste silk |
Non-Patent Citations (1)
| Title |
|---|
| 胡桂燕等: "蚕丝蛋白的提取及开发应用研究", 《2002年中国化妆品学术研讨会论文集》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106750773A (en) * | 2016-12-06 | 2017-05-31 | 宁波芸生纺织品科技有限公司 | A kind of biological packing material and preparation method thereof |
| CN107216376A (en) * | 2017-07-28 | 2017-09-29 | 浙江理工大学 | A kind of extracting method of tussah silk fibroin |
| CN108867165A (en) * | 2018-07-23 | 2018-11-23 | 王晚秀 | A kind of preparation method of silk gum paper sizing agent |
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Application publication date: 20151104 |