CN105016842A - Chlorine-based gamma-biological nitrogenous fertilizer and preparation method for same - Google Patents
Chlorine-based gamma-biological nitrogenous fertilizer and preparation method for same Download PDFInfo
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Abstract
The invention discloses a chlorine-based gamma-biological nitrogenous fertilizer and a preparation method for the same. The chlorine-based gamma-biological nitrogenous fertilizer comprises gamma-poly-glutamic acid, wheat straw extract, a microbial agent and a secondary nitrogen element, wherein the gamma-poly-glutamic acid and the microbial agent are integrally prepared by a fermentation process; the wheat straw extract is obtained by extracting and processing wheat straw tender shoots; the secondary nitrogen element is obtained from related compound powder; the chlorine-based gamma-biological nitrogenous fertilizer comprises 0.1 to 5.0 parts of the gamma-poly-glutamic acid, 2 to 20 parts of the wheat straw extract, 3 to 10 parts of the microbial agent with bacterium content of more than 2 billion per gram, 60 to 80 parts of urea with N content of 46 percent and 10 to 25 parts of ammonium chloride. According to the chlorine-based gamma-biological nitrogenous fertilizer and the preparation method for the same, the utilization rate of nitrogenous nutrition can be increased; under the synergistic effect of the gamma-poly-glutamic acid and the wheat straw extract, a soil micro-environment is improved, the absorption of nutrients is promoted, the yield of crops is improved, and the disease resistance, the stress tolerance, the interference resistance and the successive cropping tolerance of plants are improved.
Description
Technical field
The present invention relates to a kind of microorganism bacterium fertilizer production technology field, particularly a kind of chloro γ-biological nitrogen fertilizer and preparation method thereof.
Background technology
In modern agricultural production, chemical fertilizer plays more and more important effect, wherein maximum with nitrogenous fertilizer consumption, every year about 3,000 ten thousand tons (pure).But, nitrogenous fertilizer is by approach such as volatilization, leaching and runoffs, average loss is up to 45%, cause the nitrogen of annual loss up to more than 1,300 ten thousand tons, be equivalent to tens million of tons of urea, more seriously, chemical fertilizer already and will continue to bring many bad consequences, as problems such as soil compaction, water pollution, pollution of agricultural products, Greenhouse effect to our living environment.
Conventional biological nitrogen fertilizer is divided into sulfenyl nitrogenous fertilizer, urine base nitrogenous fertilizer, chloro nitrogenous fertilizer; The composite fertilizer of content more than 3% of chlorion is called chlorine-based compound fertilizer, because the growth of chlorine-based compound fertilizer material to some fear-chlorion crop exists negative impact, and chlorion concentrating in soil remains in a large number, easily causes soil compaction, salinization, alkalization.Worry that using Chloride fertilizer can produce detrimentally affect to crop yield and quality.Major part bio-fertilizer manufacturing enterprise is in fertilizer process, and potassium sulfate is selected in required potassium source, or sloughs the chlorion in Repone K, makes so-called S-based compound fertilizer.S-based compound fertilizer complicated process of preparation, production cost is high, and price is also higher, adds the production cost of agricultural-food.Chloro fertilizer is relative to sulfenyl fertilizer, and low cost of manufacture, selling price is also starkly lower than sulfenyl fertilizer.Reduce burden for increasing farmers' income will play a positive role, therefore developing high-quality chloro fertilizer is that people expected.
According to scientific experiment, chlorine is one of necessary seven kinds of trace elements of plant-growth, chlorine element can enter vegetable cell by the photosynthesis of involved in plant, play the osmotic pressure of regulating plant cell and the startup of pore and close, be conducive to the absorption of moisture and nutrient, improve its drought-resistant ability, chlorion has effect such as promotion photosynthesis and processbearing astrocyte etc., and it is particularly suitable to use fibre crops such as crudefiber crops; Improve the resistance against diseases of plant, as gaeumannomyces graminis disease, the stripe rust of wheat, the stem wilt etc. of corn.Chlorion has restraining effect to the nitrobacteria in soil, can delay the conversion rate of ammonium nitrogen to nitric nitrogen, thus effectively reduces nitric nitrogen loss and denitrification loss, is that the fertilizer efficiency of nitrogenous fertilizer is lasting.Say again, the stabilizing ion in chlorine crop plants cell, to the balance of intracellular storage ion and have active effect to the absorption of the elements such as nitrogen, phosphine, potassium, calcium, magnesium, silicon, be conducive to promoting growing of plant stem-leaf.
Facts have proved, coconut, onion, spinach, celery, wild cabbage etc. are happiness chlorine crops; Beet, paddy rice, millet, Chinese sorghum, wheat, eggplant, pea, chrysanthemum etc. are the crops that chlorine-resistant is stronger; Cotton, soybean, rape, tomato, oranges and tangerines, grape, tea tree, shallot, radish etc. are the medium crops of chlorine-resistant.It is all useful and harmless for using chloro fertilizer.Especially chlorion has effect such as promotion photosynthesis and processbearing astrocyte etc., and it is particularly suitable to use fibre crops such as crudefiber crops.Because chlorion is to crudefiber crop as necessary, it can promote Fibre Development, the tenacious and persistent will of fortifying fibre.Chloro fertilizer is particularly suitable for using with topdressing as base manure.
The high-quality nitrogenous fertilizer that urea is nitrogenous height, fertilizer efficiency is fast, consumption is large.But, urea in use, easily run off by approach such as volatilization, leaching, decomposition and runoffs, cause fertilizer efficiency utilization ratio not high, according to statistics, this season utilization ratio only has about 30%, and this not only causes the waste of chemical fertilizer, and for the environment that the mankind such as air, water body soil depend on for existence.
Although chloro bio-fertilizer low cost of manufacture, produce simple advantage, but the urea fertilizer utilization ratio in simple chloro fertilizer is but very low, the chlorine element in bio-feritlizer how is made to play a positive role in promotion crop growth, the nitrogenous fertilizer in urea can be made again fully effectively to be utilized, improve slowly-releasing usefulness and the permanent mechanism of chloro biological nitrogen fertilizer, thus play chlorine without element, the multiple positive effect of nitrogen element and bio-bacterial manure, associating promotion plant growth, improving edatope is the problem that people expect to research and develop, manufacture the expectation that the biological nitrogen fertilizer with same performance and effect is market and peasant.
Gamma-polyglutamic acid-(γ-poly-glutamic acid, be called for short γ-PGA) be the anionic polyamino acid that fermentable produces, environmentally safe, for green bio product, the biodegradability that tool is splendid, film-forming properties, become fibering, plasticity-, cohesiveness, the physics and chemistry of many uniquenesses such as moisture retention and biological characteristics, these characteristics make it have thickening, emulsification, gel, film forming, insulation, slowly-releasing, the beneficial functions such as hydrotropy and bonding, make it in agricultural, medicine, environment protection, food, the numerous areas such as makeup have very wide application prospect.
At China and even world wide, herbaceous plant is extended familys, and aboundresources is of a great variety; Wherein bromegrass, oat, barley are improved seeds wherein, fast growth, the solid granulates material extracted from wheat straw class leaf, there is abundant amino acid, SOD enzyme, trace element, mineral substance and a large amount of microorganism species, it is the synergistic substance that a class is excellent, coordinate with gamma-polyglutamic acid-, the advantage of the two can be given full play of, common utilization ratio and the fertilizer efficiency rate improving bio-feritlizer.
The production of gamma-polyglutamic acid-can have the technology of biological fermentation to produce on a large scale, the nitrogen nutrition utilization ratio of fertilizer can be improved, certain water retention is had again to plant, the water consumption needed for plant-growth can be reduced, but as the functional additive of suitable fertilizer, its effect and efficiency are also far from bringing into play completely; How to screen and can produce synergistic material with gamma-polyglutamic acid-, and a kind of bio-feritlizer of joint development, thus improve the useful effect of gamma-polyglutamic acid-, for country and peasant provide more effectively and the microbial-bacterial fertilizer saved, thus create larger Social benefit and economic benefit.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of chloro γ-biological nitrogen fertilizer and preparation method thereof, for the deficiency of existing fertilizer, adopt gamma-polyglutamic acid-and wheat straw extract as the complements of microbial-bacterial fertilizer synergy, gamma-polyglutamic acid-and wheat straw extract is utilized to carry out chelating to chlorine-based substances and urea and trace element, realize low cost production processing mixture, produce a kind of chloro γ-biological nitrogen fertilizer, meet the need of market.
For achieving the above object, technical scheme of the present invention is as follows: a kind of chloro γ-biological nitrogen fertilizer and preparation method thereof, comprises the preparation method of chloro γ-biological nitrogen fertilizer and chloro γ-biological nitrogen fertilizer, it is characterized in that:
Described chloro γ-biological nitrogen fertilizer comprises: functional ingredient is gamma-polyglutamic acid-, wheat straw extract, microbiobacterial agent; Moderate-element nitrogen element; It is standby that described gamma-polyglutamic acid-and microbiobacterial agent pass through zymotechnique integration system, and described wheat straw extract is extracted by the tender seedling of wheat straw and processes, and described moderate-element nitrogen element is obtained by related compounds powder.
Described gamma-polyglutamic acid-is gamma-polyglutamic acid-sterling or gamma-polyglutamic acid-salt sterling or the fermenting culture comprising gamma-polyglutamic acid-or gamma-polyglutamic acid-solution or the powder containing gamma-polyglutamic acid-; The consumption of gamma-polyglutamic acid-salt sterling, the fermenting culture comprising gamma-polyglutamic acid-, gamma-polyglutamic acid-solution, powder containing gamma-polyglutamic acid-is in wherein contained gamma-polyglutamic acid-; The molecular-weight average of gamma-polyglutamic acid-is 5-3000KDa.Preferred gamma-polyglutamic acid-solution.
Described wheat straw extract is bromegrass, barley, oat, blue or green, the tender seedling extract of buckwheat; Preferred Fructus Hordei Vulgaris extract.
Described microbiobacterial agent contains: subtilis (Bacillus subtilis) NX-2, Bacillus licheniformis (Bacillus licheniformis) and vinelandii; Microbiobacterial agent every gram more than 2,000,000,000.
Described nitrogen can derive from any two in urea (CO (NH2) 2), bicarbonate of ammonia (NH4CO3), ammonium chloride (NH4) Cl, but is not limited to above-mentioned nitrogenous fertilizer, preferred urea and ammonium chloride.
The screening formulation of described chloro γ-biological nitrogen fertilizer is: gamma-polyglutamic acid-, wheat straw extract, microbiobacterial agent, urea and ammonium chloride.
The each composition proportion of described chloro γ-biological nitrogen fertilizer is: gamma-polyglutamic acid-0.1-5.0 parts; Wheat straw extract 2-20 parts; Microbiobacterial agent 3-10 parts; 60-80 parts, leaded wastewater 46% urea, ammonium chloride 10-25 parts.
The fermentation preparation of described gamma-polyglutamic acid-:
Gamma-polyglutamic acid-is that pure biological fermentation drying forms, fermented bacterium is subtilis and Bacillus licheniformis, two kinds of bacterium are all gamma-polyglutamic acid-superior strains, utilize high yield effect of these two kinds of bacterial strains, by fermentation for gamma-polyglutamic acid-solution, further separating-purifying prepares gamma-polyglutamic acid-powder.
The purifying technique of preparing of gamma-polyglutamic acid-is: contain in the fermentor tank of sterilising medium by bacillus licheniformis and subtilis access respectively, control temperature, PH, mixing speed and ventilation dissolved oxygen, fermentation certain hour obtains the fermented liquid containing polyglutamic acid; Gamma-polyglutamic acid-fermented liquid adds diatomite, and Plate Filtration extracts supernatant liquor after being separated and removing thalline, and supernatant liquor adds ethanol alcohol precipitation and obtains gamma-polyglutamic acid-precipitation, and washing and precipitating, isolates precipitation, with deionized water dissolving, obtains gamma-polyglutamic acid-solution; By two kinds can be obtained containing bacterium gamma-polyglutamic acid-mixing solutions containing fermented liquid mixing.Wherein, the content of gamma-polyglutamic acid-is 8-30wt%, and the molecular-weight average of gamma-polyglutamic acid-is 200-3000Kda.Preferred mass percent scope is 10-20wt%.
Described subtilis and Bacillus licheniformis strain, come from China Committee for Culture Collection of Microorganisms's common micro-organisms center, CGMCC, deposit number is respectively: CGMCC NO.0833, CGMCC NO.3336.
Consisting of of described fermentable bacterium liquid: the fermented liquid of Bacillus licheniformis (Bacilluslicheniformis), subtilis (Bacillus subtilis) NX-2 bis-kinds of bacterial strains mixes with the mass ratio of 1:1.
Described gamma-polyglutamic acid-fermentation purifying process flow process:
(a) slant activation: the Bacillus licheniformis that 4 DEG C of inclined-planes are preserved, bacillus subtilis strain activation, after distinguish streak inoculation on LB solid plate substratum, cultivation 24-48h;
B prepared by () seed liquor: the bacterium colony of picking activation, is inoculated in LB liquid nutrient medium respectively, 30-37 DEG C, 160rpm cultivates 20-40h;
(c) fermentation culture: cultured seed liquor is seeded to fermention medium with the inoculum size of 10% respectively, 30-37 DEG C, rotating speed 280rpm, ventilating ratio 1:1, collect zymocyte liquid after the lichen bacillus ferments 24h-48h, after bacillus subtilis strain activation fermentation 20-40h, collect zymocyte liquid;
The fermention medium of described subtilis consists of: by percentage to the quality, 1% glucose, 0.6% dipotassium hydrogen phosphate, 0.3% potassium primary phosphate, 0.15% urea, 0.5% yeast powder, 0.41 ‰ magnesium sulfate, and all the other are water, pH7.0-7.2; The fermention medium of described Bacillus licheniformis consists of: by percentage to the quality, 2% sucrose, 0.4% extractum carnis, 2% Sodium Glutamate, 1.2%MgSO47H20,0.06%K2HPO43H2O, and all the other are water, pH7.5.
(d) gamma-polyglutamic acid-solution: two kinds of gamma-polyglutamic acid-zymocyte liquids will be obtained, first fermented liquid pH is adjusted to be 2-5 with sulfuric acid, the diatomite adding 1-8% stirs, degerming through Plate Filtration, regulates pH to 6.0-8.0 can obtain gamma-polyglutamic acid-solution with 30 ~ 40%NaOH;
(e) separating-purifying: respectively by two kinds of gamma-polyglutamic acid-solution through alcohol settling, after separation cleaning, add deionized water dissolving, prepare gamma-polyglutamic acid-solution;
F () diatomite is fixed: the diatomite adding 5% in two kinds of zymocyte liquids that step (c) is collected respectively is with fixing thalline and promote thalline dormancy, can obtain the gamma-polyglutamic acid-mixed solution containing microbial inoculum;
(e) mixed bacteria liquid: by described in step (f) two kinds containing microbial inoculum mixed solution with the quality of 1:1 than mixing and stirring, the gamma-polyglutamic acid-mixed solution containing subtilis and Bacillus licheniformis can be obtained.
Utilize subtilis NX-2 and Bacillus licheniformis to prepare gamma-polyglutamic acid-and salt thereof, can generate 40-60g/l gamma-polyglutamic acid-, productivity is up to 1.0-3.5gh
-1l
-1.
The preparation method of described wheat straw extract:
Described wheat straw extract, be from the tender seedling of fresh wheat straw through immersion, sterilizing, cleaning, chopping, defibrination, squeeze the juice, concentrate, powder prepared by vacuum-drying, granulation process; Containing each seed amino acid, plant high-quality protein, multiple organic state micro element, various active enzyme, SOD enzyme, multiple inner complex in described wheat straw extract; Therefore, can to carry out effectively with gamma-polyglutamic acid-, multimachine, complementary synergy, promote plant absorption; Because wheat straw extract is high enrichment powder, described various component all comes from wheat straw vegetable cell, the various organic trace elements of institute's enrichment by the synergy of gamma-polyglutamic acid-, will to be easier to absorb by plant and utilization; Can be there is enzymeization reaction in contained various active enzyme and the existing gamma-polyglutamic acid-of SOD enzyme, enter and strengthen plant-growth and volume increase; Under microorganism species effect, multiple amino acids also can be polymerized and condensation reaction with gamma-polyglutamic acid-, increases fertilizer efficiency further, delays fertility release, protection fertility runs off; And the soluble fiber in wheat straw extract, solidification can be produced with gamma-polyglutamic acid-and genus bacillus mixture, further the distribution of dispersion effective constituent, thus increase the long-acting of fertilizer.
Containing 70 several mineral materials in wheat straw extract, except the nitrogen in plant, phosphorus, potassium, be also rich in as calcium, magnesium, iron, zinc, chromium, selenium and other trace elements; Containing 20 multiple amino acids and VITAMIN in extract; Containing hundreds of active enzyme and mushroom, wherein important have: superoxide dismutase (SOD enzyme), Terminal oxidase, amylase, decomposes the enzyme of amyloclastic, vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria; These enzymes and mushroom and chloro γ-biological nitrogen fertilizer acting in conjunction in plant, thus improve fertilizer efficiency, and stimulating plant grows.
Described wheat straw extract preparation technology flow process:
(1) sterilizing is soaked: the tender seedling of wheat straw of gathering, is soaked in salt solution, and soak time is 1-4 hours; Concentration of salt solution is 4-12%; Salts solution temperature is 10-25 DEG C;
(2) clean: after being taken out by the tender seedling of wheat straw soaked after sterilizing, repeatedly clean with tap water, control is except moisture content;
(3) shred: tender for the wheat straw after cleaning seedling is sent into knife mill, by tender for wheat straw seedling chopping, chopping segment length is 2-6cm;
(4) defibrination: the tender seedling of wheat straw after chopping is carried out low temperature defibrination by paste roller mill, obtains wheat straw slurry;
(5) squeeze the juice: wheat straw slurry is sent into type juice extractor and squeezes the juice, obtain the fresh juice of wheat straw;
(6) concentrated: fresh for wheat straw juice is sent into vacuum cycle vaporizer and carries out cryoconcentration, concentration ratio is 8:1-12:1; Vacuum cycle vaporization temperature is lower than 45 DEG C;
(7) vacuum-drying: wheat straw juice concentrated solution is sent into vacuum drier, carries out low-temperature vacuum drying, obtained wheat straw solid substance;
(8) granulation: wheat straw solid substance is carried out circulation granulation by rolling pelletizer, obtains wheat straw extract powder.
Chloro γ-biological nitrogen fertilizer preparation method:
A. comminuting matter: urea, ammonium chloride are pulverized respectively, sieves the urea after pulverizing, ammonium chloride, makes the granularity of urea, ammonium chloride for subsequent use in 200-300 order scopes;
B. according to formula rate, by the gamma-polyglutamic acid-powder of preparation and wheat straw extract ground and mixed, short run powder mixes, and uses 3 D stereo mixing and grinding machine, and obtained mixed powder is for subsequent use; Microbiobacterial agent is dissolved in gamma-polyglutamic acid-mixed solution for subsequent use.
C. two mixing: mixed by drum mixer with urea by the mixed powder in step b, gained mixed powder is mixed by drum mixer with ammonium chloride powder again, obtains biological nitrogen fertilizer body;
D. granulation: by bio-feritlizer body obtained in step c, dissolve secondary by high tower and mend nitrogen granulation, screening, obtains biological nitrogen fertilizer particle;
E. pack: by the biological nitrogen fertilizer particle transport that obtains in steps d to automatic-packaging, metering packing becomes refined biometric nitrogenous fertilizer.
By technique scheme, the beneficial effect of technical solution of the present invention is:
1, chloro γ-biological nitrogen fertilizer can make original fertilizer efficiency improve 30-50%, and the regulation and control of the positive energy of involved in plant Growth of Cells, promote the nitrogen fixing capacity of crop self and the ability of enrichment nutrient, thus promote plant disease-resistant, degeneration-resistant, anti-interference, anti-continuous cropping ability.
2, chloro γ-biological nitrogen fertilizer has the function of protection root hair; more effectively can improve the dissolving of fertilizer, storage, conveying and absorption; the bio-synergistic factor contains growth hormone, the phytokinin that Institute of Micro-biology produces; effectively can promote the growth of root system; make it to grow longer root and more hairs; increase the table volume of root, stem, leaf, improve the ability of Crop nutrient, thus greatly improve the output of crop.Effective prevention vitriol, phosphoric acid salt, oxalate and middle micro-metals produce precipitating action, make the more effective absorption Trace Elements in Soil of crop energy.
3, act synergistically described in chloro γ-biological nitrogen fertilizer wheat straw extract and gamma-polyglutamic acid-action compensating, the a large amount of microorganisms in wheat straw extract, amino acid, SOD and various trace element and gamma-polyglutamic acid-is made jointly to form the effect of coating, chelating urea, extend fertilizer efficiency action time, reduce the hydrolysis of urea, reduce the volatilization loss of ammonia, single fertilization can be realized need not topdress, saving of work and time in agriculture production, significantly can also improve crop yield simultaneously.
4, chloro γ-biological nitrogen fertilizer can improve Soil Micro-environment, activating soil, can increase the beneficial microorganism in soil, thus improves the acid because life-time service chemical fertilizer causes, the soil property that alkalizes and soil compaction; The present invention has superpower chelate heavy metals ability, thus crop can be made to avoid nitrate and harmful heavy metal ions in hyperabsorption soil.
5, chloro γ-biological nitrogen fertilizer can Enriching soil nutrient, remarkable increase yield: be about 100 times of natural soils with the exchange capacity of absorption of nutrient ion, tool stores function to the absorption chelating of nutrient and suction, leaching loss and the volatilization of nutrient can be decreased, while enrichment N P and K, be also enriched micro-nutrient in calcium, magnesium, molybdenum, zinc etc., meet many-sided demand of crop, field crop, under normal weather conditions, under subtracting the condition of amount of nitrogen 50%, on average can increase production about 8-30%.
6, chloro γ-biological nitrogen fertilizer activates water retention property, promote water conservation and the drought-resistant ability of soil, significantly improve utilization rate of fertilizer: this product contains a large amount of hydrophilic radical (1600 times), can effectively pin soil moisture and fertility, promote the water conservation of soil and drought-resistant ability, strengthen crop drought resistance, the anti-adversity ability such as cold-resistant, disease-resistant.Improve active water and fertilizer slow release and utilization rate of fertilizer 30-50%, the environmental pollution that excessive or improper fertilising and loss etc. cause can be reduced, the low-carbon (LC) of promotion China agricultural, environmental protection, energy-conservation and Sustainable development.
7, the gamma-polyglutamic acid-in the present invention and wheat straw extract can produce a kind of synergy, gamma-polyglutamic acid-biomacromolecule can adsorb wheat straw extract and urea fertilizer by chelating, the two is made not easily to be separated, also can form layer protecting film on the two surface simultaneously, improve soil microflora, improve soil quality; Synergy fertilizer synergistic is effective, and the nitrogen nutrition of plant available slowly released by fertilizer, and do not produce and burn seedling phenomenon, can extend about 60 days urea efficiency phase, fertilizer efficiency period can reach more than 100 days.
8, gamma-polyglutamic acid-is that pure biological fermentation forms, and wheat straw extract is pure plant physical method extraction face one-tenth, all can not cause any pollution to environment, wherein containing various element and plant-growth regulator such as nitrogen, phosphorus, potassium, therefore serve the effect of nutrition, physiological regulation simultaneously, strengthen crop anti-adversity ability, be conducive to the growth of crop.
9, chloro γ-biological nitrogen fertilizer effectively improves crop quality and value: containing a large amount of VITAMIN and bioactive enzyme in described wheat straw extract, the plant transcription factor that vitamin B complex needed for growth and development of plants etc. is useful can be strengthened, therefore obviously can increase the chlorophyll of plant, VC, sugared part and protein content, remarkable lifting crop mouthfeel, sense organ, nutritional quality, thus the commodity value improving crop.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The invention provides a kind of chloro γ-biological nitrogen fertilizer and preparation method thereof, comprise the preparation method of chloro γ-biological nitrogen fertilizer and chloro γ-biological nitrogen fertilizer, it is characterized in that:
Described chloro γ-biological nitrogen fertilizer comprises: functional ingredient is gamma-polyglutamic acid-, wheat straw extract, microbiobacterial agent; Moderate-element nitrogen element; It is standby that described gamma-polyglutamic acid-and microbiobacterial agent pass through zymotechnique integration system, and described wheat straw extract is extracted by the tender seedling of wheat straw and processes, and described moderate-element nitrogen element is obtained by related compounds powder.
The screening formulation of described chloro γ-biological nitrogen fertilizer is: gamma-polyglutamic acid-, wheat straw extract, microbiobacterial agent; Urea and ammonium chloride.
The each composition proportion of described chloro γ-biological nitrogen fertilizer is: gamma-polyglutamic acid-0.5-1.0 parts; Wheat straw extract 8-16 parts; Microbiobacterial agent 3-6 parts; 45-55 parts, leaded wastewater 46% urea, ammonium chloride 10-15 parts.
The fermentation preparation of described gamma-polyglutamic acid-:
Gamma-polyglutamic acid-is that pure biological fermentation drying forms, fermented bacterium is subtilis and Bacillus licheniformis, two kinds of bacterium are all gamma-polyglutamic acid-superior strains, utilize high yield effect of these two kinds of bacterial strains, by fermentation for gamma-polyglutamic acid-solution, further separating-purifying prepares gamma-polyglutamic acid-powder.
The purifying technique of preparing of gamma-polyglutamic acid-is: enter in the fermentor tank containing sterilising medium by bacillus licheniformis and subtilis inoculation respectively, control temperature 32-35 DEG C, cultivation 25-35h, pH7.0, mixing speed 160rpm and continuous ventilating dissolved oxygen, ferment certain 30-40 hours, obtain the fermented liquid containing polyglutamic acid; Gamma-polyglutamic acid-fermented liquid adds diatomite, after Plate Filtration is separated and removes thalline, extract supernatant liquor, supernatant liquor adds ethanol alcohol precipitation and obtains gamma-polyglutamic acid-precipitation, and washing and precipitating, isolates precipitation, with deionized water dissolving, obtain gamma-polyglutamic acid-solution, drying of being dusted by gamma-polyglutamic acid-, obtains gamma-polyglutamic acid-powder; By two kinds can be obtained containing bacterium gamma-polyglutamic acid-solution containing fermented liquid mixing.Wherein, in gamma-polyglutamic acid-powder, the content of gamma-polyglutamic acid-is 10-40wt%, and the molecular-weight average of gamma-polyglutamic acid-is 200-3000Kda.Preferred mass percent scope is 12-22wt%.
Consisting of of described fermentable bacterium liquid: the fermented liquid of Bacillus licheniformis (Bacilluslicheniformis), subtilis (Bacillus subtilis) NX-2 bis-kinds of bacterial strains mixes with the mass ratio of 1:1.
Described gamma-polyglutamic acid-fermentation purification process step:
(a) slant activation: the Bacillus licheniformis that 4 DEG C of inclined-planes are preserved, bacillus subtilis strain activation, after distinguish streak inoculation on LB solid plate substratum, cultivation 25-35h;
B prepared by () seed liquor: the bacterium colony of picking activation, is inoculated in LB liquid nutrient medium respectively, 32-35 DEG C, 180rpm cultivates 30-40h;
(c) fermentation culture: cultured seed liquor is seeded to fermention medium with the inoculum size of 10% respectively, 32-35 DEG C, rotating speed 280rpm, ventilating ratio 1:1, collect zymocyte liquid after the lichen bacillus ferments 30h-40h, after bacillus subtilis strain activation fermentation 30-40h, collect zymocyte liquid;
The fermention medium of described subtilis consists of: by percentage to the quality, 1% glucose, 0.6% dipotassium hydrogen phosphate, 0.3% potassium primary phosphate, 0.15% urea, 0.5% yeast powder, 0.41 ‰ magnesium sulfate, and all the other are water, pH7.0-7.2; The fermention medium of described Bacillus licheniformis consists of: by percentage to the quality, 2% sucrose, 0.4% extractum carnis, 2% Sodium Glutamate, 1.2%MgSO47H20,0.06%K2HPO43H2O, and all the other are water, pH7.5.
(d) gamma-polyglutamic acid-solution: two kinds of gamma-polyglutamic acid-zymocyte liquids will be obtained, first fermented liquid pH is adjusted to be 4.5 with sulfuric acid, the diatomite adding 3% stirs, degerming through Plate Filtration, regulates pH to 7.0 can obtain gamma-polyglutamic acid-solution with 35%NaOH;
(e) separating-purifying: respectively by two kinds of gamma-polyglutamic acid-solution through alcohol settling, after separation cleaning, add deionized water dissolving, lysate carried out dusting drying, prepare gamma-polyglutamic acid-powder;
F () diatomite is fixed: the diatomite adding 5% in two kinds of zymocyte liquids that step (c) is collected respectively is with fixing thalline and promote thalline dormancy, can obtain the gamma-polyglutamic acid-mixed solution containing microbial inoculum;
(e) mixed bacteria liquid: by described in step (f) two kinds containing microbial inoculum mixed solution with the quality of 1:1 than mixing and stirring, the gamma-polyglutamic acid-mixed solution containing subtilis and Bacillus licheniformis can be obtained.
Described wheat straw extract step of preparation process:
Described wheat straw extract, be from the tender seedling of fresh wheat straw through immersion, sterilizing, cleaning, chopping, defibrination, squeeze the juice, concentrate, powder prepared by vacuum-drying, granulation process;
(1) sterilizing is soaked: the tender seedling of wheat straw of gathering, is soaked in salt solution, and soak time is 2 hours; Concentration of salt solution is 5-8%; Salts solution temperature is 15-20 DEG C;
(2) clean: after being taken out by the tender seedling of wheat straw soaked after sterilizing, repeatedly clean with tap water, control is except moisture content;
(3) shred: tender for the wheat straw after cleaning seedling is sent into knife mill, by tender for wheat straw seedling chopping, chopping segment length is 4-5cm;
(4) defibrination: the tender seedling of wheat straw after chopping is carried out low temperature defibrination by paste roller mill, obtains wheat straw slurry;
(5) squeeze the juice: wheat straw slurry is sent into type juice extractor and squeezes the juice, obtain the fresh juice of wheat straw;
(6) concentrated: fresh for wheat straw juice is sent into vacuum cycle vaporizer and carries out cryoconcentration, concentration ratio is 10:1; Vacuum cycle vaporization temperature is lower than 40 DEG C;
(7) vacuum-drying: wheat straw juice concentrated solution is sent into vacuum drier, carries out low-temperature vacuum drying, obtained wheat straw solid substance;
(8) granulation: wheat straw solid substance is carried out circulation granulation by rolling pelletizer, obtains wheat straw extract powder.
Chloro γ-biological nitrogen fertilizer step of preparation process:
A. comminuting matter: urea, ammonium chloride are pulverized respectively, sieves the urea after pulverizing, ammonium chloride, makes the granularity of urea, ammonium chloride for subsequent use at 250 orders;
B. according to formula rate, by the gamma-polyglutamic acid-powder of preparation and wheat straw extract ground and mixed, short run powder mixes, and uses 3 D stereo mixing and grinding machine, and obtained mixed powder is for subsequent use; Microbiobacterial agent is dissolved in gamma-polyglutamic acid-mixed solution for subsequent use.
C. two mixing: mixed by drum mixer with urea by the mixed powder in step b, the turbid conjunction powder of gained is mixed by drum mixer with ammonium chloride powder again, obtains biological nitrogen fertilizer body;
D. granulation: by bio-feritlizer body obtained in step c, dissolve secondary by high tower and mend nitrogen granulation, screening, obtains biological nitrogen fertilizer particle;
E. pack: by the biological nitrogen fertilizer particle transport that obtains in steps d to automatic-packaging, metering packing becomes refined biometric nitrogenous fertilizer.
Chloro γ-biological nitrogen fertilizer specific embodiment:
Embodiment 1: gamma-polyglutamic acid-0.5 part; Wheat straw extract 10 parts; Microbiobacterial agent 6 parts; 80 parts, urea, ammonium chloride 10 parts; Increase production the disease-resistant strong stress resistance resistant to lodging of 8-20% after rice application, quality mouthfeel is good, and objectionable impurities reduces.
Embodiment 2: gamma-polyglutamic acid-0.5 part; Wheat straw extract 8 parts; Microbiobacterial agent 3 parts; 65 parts, urea, ammonium chloride 25 parts; The general every per mu yield 1500 jin of blueberry, use rear volume increase 18%, every per mu yield can reach 1770 jin, and market value per jin is 80 yuan, and every mu can increase income 22000 yuan, and quality is better.
Embodiment 3: gamma-polyglutamic acid-0.6 part; Wheat straw extract 12 parts; Microbiobacterial agent 5 parts; 75 parts, urea, ammonium chloride 15 parts; General the yield of watermelon can increase production 23% after using at 7000-8000 jin, and every mu can reach more than 10000 jin, and the thin sand of skin sweet and tasty mouth quality is high.
Embodiment 4: gamma-polyglutamic acid-0.7 part; Wheat straw extract 14 parts; Microbiobacterial agent 4 parts; 70 parts, urea, ammonium chloride 20 parts; The general every per mu yield 12000 of cucumber, use rear increasing 25%, every per mu yield can reach more than 15000, and 2 yuan per jin of common market price, every mu increases income 6000 yuan.
Embodiment 5: gamma-polyglutamic acid-0.5 part; Wheat straw extract 10 parts; Microbiobacterial agent 7 parts; 55 parts, urea, ammonium chloride 25 parts; The general every per mu yield 12000 of tomato, use rear volume increase 28%, every per mu yield can reach more than 15000.3 yuan every mu per jin of market price increases income 9000 yuan.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (8)
1. chloro γ-biological nitrogen fertilizer and preparation method thereof, is characterized in that, comprises the preparation method of chloro γ-biological nitrogen fertilizer and chloro γ-biological nitrogen fertilizer; Described chloro γ-biological nitrogen fertilizer comprises: functional ingredient is gamma-polyglutamic acid-, wheat straw extract, microbiobacterial agent; Moderate-element nitrogen element; It is standby that described gamma-polyglutamic acid-and microbiobacterial agent pass through zymotechnique integration system, and described wheat straw extract is extracted by the tender seedling of wheat straw and processes, and described moderate-element nitrogen element is obtained by related compounds powder; The screening formulation of described chloro γ-biological nitrogen fertilizer is: gamma-polyglutamic acid-, wheat straw extract, microbiobacterial agent; Moderate-element nitrogen element; Be preferably urea and ammonium chloride; Described nitrogen element can derive from urea (CO (NH
2)
2), ammonium chloride (NH
4cl); The each composition proportion of described chloro γ-biological nitrogen fertilizer is: gamma-polyglutamic acid-0.1-5.0 parts; Wheat straw extract 2-20 parts; Microbiobacterial agent 3-10 parts; 60-80 parts, leaded wastewater 46% urea, ammonium chloride 10-25 parts.
2. a kind of chloro γ-biological nitrogen fertilizer according to claim 1 and preparation method thereof, it is characterized in that, described gamma-polyglutamic acid-is gamma-polyglutamic acid-solution or gamma-polyglutamic acid-sterling or its salt sterling or the powder comprising the fermenting culture of gamma-polyglutamic acid-or the gamma-polyglutamic acid-containing fermenting agent; In wherein contained gamma-polyglutamic acid-; The molecular-weight average of gamma-polyglutamic acid-is 5-3000KDa, preferred gamma-polyglutamic acid-solution.
3. a kind of chloro γ-biological nitrogen fertilizer according to claim 1 and preparation method thereof, is characterized in that, described wheat straw extract is bromegrass, barley, oat, blue or green, the tender seedling extract of buckwheat; Preferred Fructus Hordei Vulgaris extract.
4. a kind of chloro γ-biological nitrogen fertilizer according to claim 1 and preparation method thereof, it is characterized in that, the fermentation preparation of described gamma-polyglutamic acid-is contain in the fermentor tank of sterilising medium by bacillus licheniformis and subtilis access respectively, control temperature, PH, mixing speed and ventilation dissolved oxygen, fermentation certain hour obtains the fermented liquid containing polyglutamic acid; Gamma-polyglutamic acid-fermented liquid adds diatomite, and Plate Filtration extracts supernatant liquor after being separated and removing thalline, and supernatant liquor adds ethanol alcohol precipitation and obtains gamma-polyglutamic acid-precipitation, and washing and precipitating, isolates precipitation, with deionized water dissolving, obtains gamma-polyglutamic acid-solution; By two kinds can be obtained containing bacterium gamma-polyglutamic acid-broth solution containing fermented liquid mixing.Wherein, in gamma-polyglutamic acid-solution, the content of gamma-polyglutamic acid-is 18-30wt%, and the molecular-weight average of gamma-polyglutamic acid-is 200-3000Kda, and preferred mass percent scope is 10-20wt%.
5. a kind of chloro γ-biological nitrogen fertilizer according to claim 1 and preparation method thereof, it is characterized in that, consisting of of described fermentable bacterium liquid: Bacillus licheniformis (Bacilluslicheniformis), subtilis (Bacillus subtilis) NX-2, the fermented liquid of two kinds of bacterial strains mixes with the mass ratio of 1:1.
6. a kind of chloro γ-biological nitrogen fertilizer according to claim 1 and preparation method thereof, is characterized in that, described gamma-polyglutamic acid-fermentation purifying process flow process:
(a) slant activation: the Bacillus licheniformis that 4 DEG C of inclined-planes are preserved, bacillus subtilis strain activation, after distinguish streak inoculation on LB solid plate substratum, cultivation 24-48h;
B prepared by () seed liquor: the bacterium colony of picking activation, is inoculated in LB liquid nutrient medium respectively, 30-37 DEG C, 160rpm cultivates 20-40h;
(c) fermentation culture: cultured seed liquor is seeded to fermention medium with the inoculum size of 10% respectively, 30-37 DEG C, rotating speed 280rpm, ventilating ratio 1:1, collect zymocyte liquid after the lichen bacillus ferments 24h-48h, after bacillus subtilis strain activation fermentation 20-40h, collect zymocyte liquid;
(d) gamma-polyglutamic acid-solution: two kinds of gamma-polyglutamic acid-zymocyte liquids will be obtained, first fermented liquid pH is adjusted to be 2-5 with sulfuric acid, the diatomite adding 1-8% stirs, degerming through Plate Filtration, regulates pH to 6.0-8.0 can obtain gamma-polyglutamic acid-solution with 30 ~ 40%NaOH;
(e) separating-purifying: respectively by two kinds of gamma-polyglutamic acid-solution through alcohol settling, after separation cleaning, add deionized water dissolving, prepare gamma-polyglutamic acid-solution;
F () diatomite is fixed: the diatomite adding 5% in two kinds of zymocyte liquids that step (c) is collected respectively is with fixing thalline and promote thalline dormancy, can obtain the gamma-polyglutamic acid-mixed solution containing microbial inoculum;
(e) mixed bacteria liquid: by described in step (f) two kinds containing microbial inoculum mixed solution with the quality of 1:1 than mixing and stirring, the gamma-polyglutamic acid-mixed solution containing subtilis and Bacillus licheniformis can be obtained.
7. a kind of chloro γ-biological nitrogen fertilizer according to claim 1 and preparation method thereof, it is characterized in that, the preparation process of described wheat straw extract is: by tender for fresh wheat straw seedling through immersion, sterilizing, cleaning, chopping, defibrination, squeeze the juice, concentrate, powder prepared by vacuum-drying, granulation process; Described wheat straw extract preparation technology flow process:
(1) sterilizing is soaked: the tender seedling of wheat straw of gathering, is soaked in salt solution, and soak time is 1-4 hours; Concentration of salt solution is 4-12%; Salts solution temperature is 10-25 DEG C;
(2) clean: after being taken out by the tender seedling of wheat straw soaked after sterilizing, repeatedly clean with tap water, control is except moisture content;
(3) shred: tender for the wheat straw after cleaning seedling is sent into knife mill, by tender for wheat straw seedling chopping, chopping segment length is 2-6cm;
(4) defibrination: the tender seedling of wheat straw after chopping is carried out low temperature defibrination by paste roller mill, obtains wheat straw slurry;
(5) squeeze the juice: wheat straw slurry is sent into type juice extractor and squeezes the juice, obtain the fresh juice of wheat straw;
(6) concentrated: fresh for wheat straw juice is sent into vacuum cycle vaporizer and carries out cryoconcentration, concentration ratio is 8:1-12:1; Vacuum cycle vaporization temperature is lower than 45 DEG C;
(7) vacuum-drying: wheat straw juice concentrated solution is sent into vacuum drier, carries out low-temperature vacuum drying, obtained wheat straw solid substance;
(8) granulation: wheat straw solid substance is carried out circulation granulation by rolling pelletizer, obtains wheat straw extract powder.
8. a kind of chloro γ-biological nitrogen fertilizer according to claim 1 and preparation method thereof, is characterized in that, described chloro γ-biological nitrogen fertilizer preparation method:
A. comminuting matter: urea, ammonium chloride are pulverized respectively, sieves the urea after pulverizing, ammonium chloride, makes the granularity of urea, ammonium chloride for subsequent use in 200-300 order scopes; Microbiobacterial agent is dissolved in gamma-polyglutamic acid-mixed solution for subsequent use.
B. according to formula rate, the gamma-polyglutamic acid-mixed solution of preparation is sprayed on wheat straw extract by electrostatic atomizing type; And material is carried out ground and mixed, short run powder mixes, and uses 3 D stereo mixing and grinding machine, and obtained mixed powder is for subsequent use;
C. two mixing: mixed by drum mixer with urea by the mixed powder in step b, gained mixed powder is mixed by drum mixer with ammonium chloride powder again, obtains biological nitrogen fertilizer body;
D. granulation: by bio-feritlizer body obtained in step c, dissolve secondary by high tower and mend nitrogen granulation, screening, obtains biological nitrogen fertilizer particle;
E. pack: by the biological nitrogen fertilizer particle transport that obtains in steps d to automatic-packaging, metering packing becomes refined biometric nitrogenous fertilizer.
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