CN105012049B - The mixed atomizing printing technology of printing head system and adjustable soft hollow pipe holder - Google Patents
The mixed atomizing printing technology of printing head system and adjustable soft hollow pipe holder Download PDFInfo
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- CN105012049B CN105012049B CN201510300556.7A CN201510300556A CN105012049B CN 105012049 B CN105012049 B CN 105012049B CN 201510300556 A CN201510300556 A CN 201510300556A CN 105012049 B CN105012049 B CN 105012049B
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Abstract
The present invention provides the mixed atomizing printing technology of printing head system and adjustable soft hollow pipe holder.System includes five functional parts:Pneumatic power source, feed device, mixing arrangement, shower nozzle and sprayer unit.Solution A under solution B, solution C, solution D effect, by dispensing, mixing and spraying out technology, forms hollow circular-tube by shower nozzle;Two-layer or sandwich construction can be built, and under nebulization, bonding strength between layers can be strengthened.The present invention can be thoroughly mixed two kinds of solution, continuous hollow or solid circular pipe structure can be printed and added up and form different structure, solve the printing and manufacture of the hollow circular-tubes such as similar blood vessel, and solve to repair the manufacturing issue that bulk tissue builds internal stent micro-channel structure for materials such as transmission nutrition in organizational project, the structure of various biological tissues can be widely used in.
Description
Technical field
The present invention is mechanical automation and biotechnology, organizational project crossing domain, and in particular to printing head system and
The mixed atomizing printing technology of adjustable soft hollow pipe holder.
Background technology
Organizational project builds artificial group by simulating natural tissues structure using the element of cell, support, growth factor etc. three
Knit, for impaired or degenerate tissue reparation.Wherein, support is the key of space transoportation and positioning cell and growth factor, not only
Need with certain mechanical load-bearing capability and the property of water-bearing, and in the application towards thick tissue and organ, it is a large amount of in order to give
Cell transports enough nutrition, growth factor, oxygen, and discharges metabolic waste, cell is grown and is fused together, and is formed
Large-scale tissue and organ, but it is that its manufacture is difficult to manufacture and form the vascularization with similar blood vessel structure and blood vessel network
Point.
The structure of the hydrogels such as sodium alginate is similar to the structure of the extracellular matrix of biological tissue, with good biology
The critical natures such as compatibility, biological degradability, hypotoxicity and immunoprotection, a large amount of water being rich in inside it can be effectively cell
Growth conditions is provided, is widely used in and is prepared support, but the hydrogel inside cylinder structure of bulk is difficult to customize completion.
The content of the invention
It is an object of the invention to overcome above-mentioned technical deficiency, there is provided a kind of 3D printing system, not only can be in stamping ink
Different cells is printed upon specified location according to demand while gel stent, and is formed with hollow circular-tube structure stand
(scaffold) or cell and support complex (construct), the structure and function of bionical rete vasculosum can be effectively completed.
The method can be widely used in the structure of various biological tissues.
The technical proposal of the invention is realized in this way:
A kind of printing head system, the system includes five functional parts:Pneumatic power source, feed device, mixing arrangement,
Shower nozzle and sprayer unit, pneumatic power source are connected with feed device by pneumatic hose, and feed device is connected with mixing arrangement, are mixed
Attach together to put and be connected by pneumatic hose with shower nozzle.
The mixing arrangement uses reversion structure, including joint, the first curved pipe, and first is straight, the first U-tube, and second is bent
Shape pipe, the second U-tube, the second straight tube, the 3rd straight tube;First straight tube is connected with the first curved pipe, the first U-tube and the first straight tube
Connection, the second straight tube is connected with the first U-tube, and the second U-tube is connected with the second straight tube, and the 3rd straight tube is connected with the second U-tube,
Second curved pipe is connected with the 3rd straight tube;Inside includes the first static agitation blade, the second static agitation blade, wherein first is bent
Shape pipe is connected with joint.
The first static agitation blade is provided with first straight tube, the second straight tube, the 3rd straight tube, is provided with the first U-tube
Second static agitation blade.
Described shower nozzle includes shower nozzle one or shower nozzle two.
Mixing arrangement is connected with shower nozzle one or is connected with shower nozzle two.
A kind of mixed atomizing printing technology of adjustable soft hollow pipe holder, comprises the following steps:
1), at normal temperatures, the fluid reservoir A in feed device is moved equipped with solution B is equipped with solution A, fluid reservoir B pneumatic
In the presence of the pressed gas that power source provides, solution A is extruded with solution B by biliquid glue dispensing valve;
2) after, solution A and solution B are extruded by biliquid glue dispensing valve, into mixing arrangement, in the pressure that pneumatic power source is provided
In the presence of gas, solution A and solution B haptoreaction, and by the first static agitation blade, the second static agitation blade,
After flowing through the reversion structure of whole mixing arrangement, solution A is sufficiently mixed with solution B;
3) after, solution A and the blended pipe of solution B are sufficiently mixed, into shower nozzle one or shower nozzle two, in shower nozzle one or shower nozzle two
Exit, forms hollow or solid circular pipe structure;
4), in the exit of shower nozzle one, while forming hollow circular-tube structure, solution is clamp-oned in the syringe needle two in shower nozzle one
C, solution C is contacted with the mixture of solution A, solution B in the exit of shower nozzle one, forms the internal structure of hollow circular-tube;
5), the atomized soln D that solution D is extruded into from sprayer unit, with the formation hollow circular-tube extruded from shower nozzle one
The surface haptoreaction of the mixture of internal structure, forms the external structure of hollow pipe;
6), by above-mentioned steps be build one layer of hollow circular-tube structure, build next layer when same above-mentioned steps, solution D from
Sprayer unit is extruded into atomized soln D, with step 4) mixture reacted, i.e., connection that can be between enhancement layer and layer
Intensity.The hollow circular-tube structure of structure is two-layer or multilayer.
It is raw from the solid circular pipe structure and the atomized soln D haptoreactions being extruded into from sprayer unit of the extrusion of shower nozzle two
Into solid circular pipe structure.
The extruded velocity of solution A is 0.1-1.0ml/min, and the extruded velocity of solution B is 0.1-1.0ml/min, solution C
Extruded velocity is 40-70ul/sec, and the extruded velocity of solution D is 300-700ul/sec.
Solution A is the Native Gel material as representative with sodium alginate, shitosan, solution B be with calcium chloride or barium chloride,
NaOH is that representative can trigger sodium alginate, the solution of shitosan plastic, and solution C is with calcium chloride or barium chloride, hydrogen-oxygen
It is that representative can trigger sodium alginate, the solution of shitosan plastic to change sodium, and solution D is with calcium chloride or barium chloride, NaOH
It is the solution that can trigger sodium alginate, shitosan plastic for representing, the concentration of solution B is 0.2%-0.6% (m/v), solution C
Concentration is 2%-6% (m/v), and the concentration of solution D is 2%-6% (m/v).
The present invention provides the mixed atomizing printing technology of printing head system and adjustable soft hollow pipe holder, can make two
Kind of solution is thoroughly mixed, can print continuous hollow or solid circular pipe structure and it is cumulative form different structure, solve similar blood
The printing and manufacture of the hollow circular-tubes such as pipe, and solve to be repaired in organizational project bulk tissue for transmission nutrition etc. material structure
The manufacturing issue of internal stent micro-channel structure is built, the structure of various biological tissues can be widely used in.
Brief description of the drawings
Fig. 1 is printing head system;
1 is pneumatic power source, 2 is feed device, 3 is mixing arrangement, 4 is that shower nozzle one, 5 is that shower nozzle two, 6 is dress of spraying
Put, 7 be air compressor machine, 8 be pressure-reducing valve, 9 be biliquid glue dispensing valve, 10 be controller, 11 be fluid reservoir A, 12 be fluid reservoir B.
Fig. 2 is mixing arrangement figure.
Fig. 2 a) mixing arrangement outside drawing.
13 is joint in figure, and 14 is the first curved pipe, and 15 is the first straight tube, and 16 is the first U-tube, and 17 is second curved
Pipe, 18 is the second U-tube, and 19 is the second straight tube, and 20 is the 3rd straight tube.
Fig. 2 b) mixing arrangement internal mix blade figure.
21 is that the first static agitation blade 1,22 is the second static agitation blade.
Fig. 3 is the figure of shower nozzle one,
Fig. 3 a) it is the outline drawing of shower nozzle one,
Fig. 3 b) it is shower nozzle one end partial enlarged drawing,.
23 is syringe needle one, and 24 is syringe needle two, and 25 is syringe needle three.
Fig. 4 is print procedure figure.
26 is print platform, and 27 is the hollow circular-tube for printing;
Fig. 5 is 3D hollow cellular structure drop figures.
28 is 3D hollow cellular structure drops.
Specific embodiment
Shown in reference picture 1, pneumatic power source 1 provides power for system.The sodium alginate of 0.2%-0.6% (m/v) is placed in
In fluid reservoir A 11, the calcium chloride of 2%-6% (m/v) is placed in fluid reservoir B 12, under gas pressure effect, is filled by feeding
Put 2 and flow through mixing arrangement 3, complete the sodium alginate of 0.2%-0.6% (m/v) and the abundant of the calcium chloride of 2%-6% (m/v) is mixed
Close.The sodium alginate of 0.2%-0.6% (m/v) is with the calcium chloride mixture of 2%-6% (m/v) by syringe needle one (23), 2%-
6% (m/v) calcium chloride passes through syringe needle 2 24, in the sodium alginate and 2%-6% of the end 0.2%-0.6% (m/v) of shower nozzle 1
(m/v) the calcium chloride contact of calcium chloride mixture and 2%-6% (m/v) chemically reacts, and forms hollow circular-tube internal junction
Structure.The calcium chloride of 2%-6% (m/v) sprays from sprayer unit (6), sodium alginate and 2%- with 0.2%-0.6% (m/v)
The calcium chloride mixture contact of 6% (m/v) chemically reacts, and forms hollow circular-tube external structure, ultimately forms hollow circular-tube.
Shown in reference picture 2, the sodium alginate of 0.2%-0.6% (m/v) is with the calcium chloride of 2%-6% (m/v) by mixing
Device 3, by the reversion structure of mixing arrangement 3, first static agitation blade 21, the second static agitation in the inside of mixing arrangement 3
Stirred in the presence of blade 22, the sodium alginate for completing 0.2%-0.6% (m/v) is abundant with the calcium chloride of 2%-6% (m/v)
Mixing.
Shown in reference picture 3, the sodium alginate of 0.2%-0.6% (m/v) leads to the calcium chloride mixture of 2%-6% (m/v)
Syringe needle 1 is crossed, the calcium chloride of %-6% (m/v) passes through syringe needle 2 24, the end 0.2%-0.6%'s (m/v) of shower nozzle 1
Sodium alginate is contacted and chemically reacted with the calcium chloride mixture of 2%-6% (m/v) and the calcium chloride of 2%-6% (m/v), shape
Into hollow circular-tube internal structure.
Shown in reference picture 4, in sodium alginate and the 2%-6% (m/v) of the end 0.2%-0.6% (m/v) of shower nozzle 1
The calcium chloride contact of calcium chloride mixture and 2%-6% (m/v) chemically reacts, and forms hollow circular-tube internal structure.2%-
The calcium chloride of 6% (m/v) sprays from sprayer unit 6, with the sodium alginate of 0.2%-0.6% (m/v) with 2%-6%'s (m/v)
Calcium chloride mixture contact chemically reacts, and forms hollow circular-tube external structure, ultimately forms hollow circular-tube.
Shown in reference picture 5, first in the sodium alginate and 2%-6% (m/v) of the end 0.2%-0.6% (m/v) of shower nozzle 1
Calcium chloride mixture and 2%-6% (m/v) calcium chloride contact chemically react, formed hollow circular-tube internal structure.
The calcium chloride of 2%-6% (m/v) sprays from sprayer unit 6, sodium alginate and 2%-6% (m/ with 0.2%-0.6% (m/v)
V) calcium chloride mixture contact chemically reacts, and forms hollow circular-tube external structure, ultimately forms hollow circular-tube.In printing
Plane forms one layer of structure being made up of hollow circular-tube, when the moment that the hollow circular-tube extruded from shower nozzle 1 is contacted with ground floor,
The atomization calcium chloride sprayed from sprayer unit 6 is chemically reacted with it, forms hollow circular-tube external structure, and strengthen last layer
With the bonding strength between next layer, 3D hollow cellular structure drops are finally constituted.
In print procedure, smooth muscle cell can be added in the sodium alginate of 0.2%-0.6% (m/v), in 2%-6%
(m/v) calcium chloride connects middle mixing endothelial cell, the hollow circular-tube inner structural wall of final printing is mixed with endothelial cell, circular pipe pipe
Wall is contained within smooth muscle cell, is cultivated in nutrient solution, completes smooth muscle cell and is co-cultured with endothelial cell, solves in tissue work
Bulk soft tissue structure is repaired in journey needs the manufacturing issue of class blood vessel structure.
Claims (9)
1. a kind of nozzle system for printing adjustable soft hollow pipe holder, the system includes five functional parts:Pneumatic power source
(1), feed device (2), mixing arrangement (3), shower nozzle and sprayer unit (6), it is characterised in that pneumatic power source (1) and feeding
Device (2) is connected by pneumatic hose, and feed device (2) is connected with mixing arrangement (3), and mixing arrangement (3) passes through gas with shower nozzle
Dynamic flexible pipe connection, mixing arrangement (3) use reversion structure, including joint (13), the first curved pipe (14), the first straight tube (15),
First U-tube (16), the second curved pipe (17), the second U-tube (18), the second straight tube (19), the 3rd straight tube (20);First straight tube
(15) it is connected with the first curved pipe (14), the first U-tube (16) is connected with the first straight tube (15), the second straight tube (19) and a U
Shape pipe (16) is connected, and the second U-tube (18) is connected with the second straight tube (19), and the 3rd straight tube (20) is connected with the second U-tube (18),
Second curved pipe (17) is connected with the 3rd straight tube (20);Inside includes the first static agitation blade (21), the second static agitation leaf
Piece (22), wherein the first curved pipe (14) is connected with joint (13).
2. the nozzle system of the adjustable soft hollow pipe holder of printing according to claim 1, it is characterised in that the first straight tube
(15) the first static agitation blade (21), is provided with the second straight tube (19), the 3rd straight tube (20), the first U-tube sets in (16)
It is equipped with the second static agitation blade (22).
3. the nozzle system of the adjustable soft hollow pipe holder of printing according to claim 1, it is characterised in that described spray
Head includes shower nozzle one (4) or shower nozzle two (5).
4. the nozzle system of the adjustable soft hollow pipe holder of printing according to claim 1 or 3, it is characterised in that mixing
Device (3) is connected with shower nozzle one (4) or is connected with shower nozzle two (5).
5. a kind of mixed atomizing printing technology for printing adjustable soft hollow pipe holder, it is characterised in that comprise the following steps:
1), at normal temperatures, the fluid reservoir A (11) in feed device (2) equipped with solution A, fluid reservoir B (12) be equipped with solution B,
In the presence of the pressed gas that pneumatic power source (1) is provided, solution A is extruded with solution B by biliquid glue dispensing valve (9);
2) after, solution A and solution B are extruded by biliquid glue dispensing valve (9), into mixing arrangement (3), provided in pneumatic power source (1)
Pressed gas in the presence of, solution A and solution B haptoreaction, and by the first static agitation blade (21), second is static
Stirring vane (22), after flowing through the reversion structure of whole mixing arrangement (3), solution A is sufficiently mixed with solution B;
3) after, solution A and the blended pipe of solution B are sufficiently mixed, into shower nozzle one (4) or shower nozzle two (5), in shower nozzle one (4) or
The exit of shower nozzle two (5), forms hollow or solid circular pipe structure;
4), in the exit of shower nozzle one (4), while forming hollow circular-tube structure, squeezed in the syringe needle two (24) in shower nozzle one (4)
Enter solution C, solution C is contacted with the mixture of solution A, solution B in the exit of shower nozzle one (4), form the internal junction of hollow circular-tube
Structure;
5), solution D is extruded into atomized soln D from sprayer unit (6), with the formation hollow circular-tube extruded from shower nozzle one (4)
The surface haptoreaction of the mixture of internal structure, forms the external structure of hollow pipe;
6) it is, to build one layer of hollow circular-tube structure by above-mentioned steps, same above-mentioned steps when building next layer, solution D is from spraying
The atomized soln D that device (6) is extruded into, with step 4) mixture reacted, i.e., company that can be between enhancement layer and layer
Connect intensity;
Wherein, solution A is sodium alginate, shitosan, and solution B, solution C and solution D are calcium chloride or barium chloride, hydroxide
Sodium.
6. the mixed atomizing printing technology of adjustable soft hollow pipe holder according to claim 5, it is characterised in that build
Hollow circular-tube structure be two-layer or multilayer.
7. the mixed atomizing printing technology of adjustable soft hollow pipe holder according to claim 5, it is characterised in that from spray
The solid circular pipe structure of first two (5) extrusions and the atomized soln D haptoreactions being extruded into from sprayer unit (6), generate solid
Circular tube structure.
8. the mixed atomizing printing technology of adjustable soft hollow pipe holder according to claim 5, it is characterised in that solution
The extruded velocity of A is 0.1-1.0ml/min, and the extruded velocity of solution B is 0.1-1.0ml/min, and the extruded velocity of solution C is
40-70ul/sec, the extruded velocity of solution D is 300-700ul/sec.
9. the mixed atomizing printing technology of adjustable soft hollow pipe holder according to claim 5, it is characterised in that solution
A is the Native Gel material as representative with sodium alginate, shitosan, and solution B is as generation with calcium chloride or barium chloride, NaOH
Table can trigger sodium alginate, the solution of shitosan plastic, and solution C is as representative with calcium chloride or barium chloride, NaOH
Sodium alginate, the solution of shitosan plastic can be triggered, solution D is can draw as representative with calcium chloride or barium chloride, NaOH
Hair sodium alginate, the solution of shitosan plastic, the concentration of solution B is 0.2%-0.6% (m/v), and the concentration of solution C is 2%-
6% (m/v), the concentration of solution D is 2%-6% (m/v).
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CN105524831B (en) * | 2015-12-29 | 2017-09-12 | 西安交通大学 | A kind of biological 3D printing culture integral system and method |
CN105796206B (en) * | 2016-05-07 | 2017-08-04 | 浙江大学 | The biological 3D printing equipment of many shower nozzles of a kind of automatic blending and batch mixing and control method |
CN109774118B (en) * | 2019-03-21 | 2020-12-29 | 中国科学院福建物质结构研究所 | Method for enhancing mechanical property of FDM3D printing part |
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US3406052A (en) * | 1963-01-28 | 1968-10-15 | Stapling Machines Co | Method of making plastic-coated corrugated fiberboard |
CN1176797C (en) * | 1999-08-18 | 2004-11-24 | 仲伟虹 | Equipment and process for layer manufacture |
DE19963948A1 (en) * | 1999-12-31 | 2001-07-26 | Zsolt Herbak | Model making process |
US7341630B1 (en) * | 2003-06-26 | 2008-03-11 | Advanced Cardiovascular Systems, Inc. | Stent coating system |
CN103182840B (en) * | 2011-12-29 | 2015-01-28 | 上海富奇凡机电科技有限公司 | Micro injector type multi-spray-head three-dimensional printing machine |
CN203713252U (en) * | 2014-01-27 | 2014-07-16 | 上海泰威技术发展股份有限公司 | Moisturizing device for surface of nozzle |
CN103923522B (en) * | 2014-04-03 | 2016-05-04 | 东华大学 | A kind of for rapid three dimensional printing forming nylon 6 inks and application thereof |
CN103932368B (en) * | 2014-04-09 | 2015-10-28 | 西安交通大学 | A kind of laser 3D food printing equipment and Method of printing |
CN103919629B (en) * | 2014-04-18 | 2016-09-28 | 清华大学 | A kind of toughness organizational structure and 3D printing-forming equipment thereof and method |
CN104552944B (en) * | 2014-12-19 | 2017-03-01 | 机械科学研究总院先进制造技术研究中心 | A kind of 3D printing dispensing extrusion device that can achieve online alloying |
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Effective date of registration: 20210628 Address after: 710049 No. 28 West Xianning Road, Shaanxi, Xi'an Patentee after: XI'AN JIAOTONG University Address before: 710049 No. 28 West Xianning Road, Shaanxi, Xi'an Patentee before: XI'AN JIAOTONG University Patentee before: SHAANXI HENGTONG INTELLIGENT MACHINE Co.,Ltd. |